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immunofluorescence if staining  (Human Protein Atlas)

 
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    Human Protein Atlas immunofluorescence if staining
    Immunofluorescence If Staining, supplied by Human Protein Atlas, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/immunofluorescence+staining/ihc+staining/pm42242683-97-55-64
    Average 86 stars, based on 1 article reviews
    immunofluorescence if staining - by Bioz Stars, 2026-09
    86/100 stars

    Images

    Related Articles

    Biomarker Discovery:

    Article Title: A 5mC-related gene signature predicts prognosis and therapy response in hepatocellular carcinoma.
    Article Snippet: .. Experimental validation of key gene expression and biological functions (A-E) Expression analysis of DNMT1, SPATS2, and MCM6 mRNA between HCC tissues and adjacent non-tumor tissues based on data from TCGA, ICGC, and GEO databases. (F) Representative IHC staining of DNMT1, SPATS2, and MCM6 in normal and HCC tissues from the Human Protein Atlas (HPA, https://www.proteinatlas.org/). ..

    Gene Expression:

    Article Title: A 5mC-related gene signature predicts prognosis and therapy response in hepatocellular carcinoma.
    Article Snippet: .. Experimental validation of key gene expression and biological functions (A-E) Expression analysis of DNMT1, SPATS2, and MCM6 mRNA between HCC tissues and adjacent non-tumor tissues based on data from TCGA, ICGC, and GEO databases. (F) Representative IHC staining of DNMT1, SPATS2, and MCM6 in normal and HCC tissues from the Human Protein Atlas (HPA, https://www.proteinatlas.org/). ..

    Expressing:

    Article Title: A 5mC-related gene signature predicts prognosis and therapy response in hepatocellular carcinoma.
    Article Snippet: .. Experimental validation of key gene expression and biological functions (A-E) Expression analysis of DNMT1, SPATS2, and MCM6 mRNA between HCC tissues and adjacent non-tumor tissues based on data from TCGA, ICGC, and GEO databases. (F) Representative IHC staining of DNMT1, SPATS2, and MCM6 in normal and HCC tissues from the Human Protein Atlas (HPA, https://www.proteinatlas.org/). ..

    Article Title: Low KIF26B Expression Reduces Paclitaxel Resistance and Predicts Good Prognosis in Ovarian Cancer.
    Article Snippet: .. KIF26B protein expression levels were assessed using IHC staining data retrieved from the Human Protein Atlas database. ..

    Article Title: C1orf226 Promotes Glioma Stemness and Oncogenesis in Glioma via PLK1-Mediated Wnt/β-Catenin Signaling.
    Article Snippet: .. Images of immunohistochemical (IHC) staining to validate C1orf226 protein expression were obtained from the Human Protein Atlas (HPA) [21]. ..

    Immunohistochemistry:

    Article Title: A 5mC-related gene signature predicts prognosis and therapy response in hepatocellular carcinoma.
    Article Snippet: .. Experimental validation of key gene expression and biological functions (A-E) Expression analysis of DNMT1, SPATS2, and MCM6 mRNA between HCC tissues and adjacent non-tumor tissues based on data from TCGA, ICGC, and GEO databases. (F) Representative IHC staining of DNMT1, SPATS2, and MCM6 in normal and HCC tissues from the Human Protein Atlas (HPA, https://www.proteinatlas.org/). ..

    Article Title: Low KIF26B Expression Reduces Paclitaxel Resistance and Predicts Good Prognosis in Ovarian Cancer.
    Article Snippet: .. KIF26B protein expression levels were assessed using IHC staining data retrieved from the Human Protein Atlas database. ..

    Article Title: CCL chemokines as prognostic and immunotherapeutic markers in prostate cancer: a comprehensive analysis.
    Article Snippet: .. IHC staining results from the Human Protein Atlas (HPA) database were retrieved to confirm the differentially expressed of CCL protein. ..

    Article Title: C1orf226 Promotes Glioma Stemness and Oncogenesis in Glioma via PLK1-Mediated Wnt/β-Catenin Signaling.
    Article Snippet: .. Images of immunohistochemical (IHC) staining to validate C1orf226 protein expression were obtained from the Human Protein Atlas (HPA) [21]. ..

    Staining:

    Article Title: A 5mC-related gene signature predicts prognosis and therapy response in hepatocellular carcinoma.
    Article Snippet: .. Experimental validation of key gene expression and biological functions (A-E) Expression analysis of DNMT1, SPATS2, and MCM6 mRNA between HCC tissues and adjacent non-tumor tissues based on data from TCGA, ICGC, and GEO databases. (F) Representative IHC staining of DNMT1, SPATS2, and MCM6 in normal and HCC tissues from the Human Protein Atlas (HPA, https://www.proteinatlas.org/). ..

    Article Title: Therapeutic small molecules for treatment of pulmonary hypertension
    Article Snippet: .. Additionally, based on lung staining data from the Human Protein Atlas (https://www.proteinatlas.org), most vascular galectin-8 is localized in endothelial cells, supporting the notion of a connection between I-BET and this C15 gene in this cell type. ..

    Article Title: Low KIF26B Expression Reduces Paclitaxel Resistance and Predicts Good Prognosis in Ovarian Cancer.
    Article Snippet: .. KIF26B protein expression levels were assessed using IHC staining data retrieved from the Human Protein Atlas database. ..

    Article Title: CCL chemokines as prognostic and immunotherapeutic markers in prostate cancer: a comprehensive analysis.
    Article Snippet: .. IHC staining results from the Human Protein Atlas (HPA) database were retrieved to confirm the differentially expressed of CCL protein. ..

    Article Title: C1orf226 Promotes Glioma Stemness and Oncogenesis in Glioma via PLK1-Mediated Wnt/β-Catenin Signaling.
    Article Snippet: .. Images of immunohistochemical (IHC) staining to validate C1orf226 protein expression were obtained from the Human Protein Atlas (HPA) [21]. ..

    other:

    Article Title: Predicting glioma survival and extracellular matrix remodeling through MRI radiogenomics
    Article Snippet: The protein expression patterns of radiogenomics-based hub genes (A) The protein expression patterns of 7 hub genes between normal brain tissues and glioma were determined by immunohistochemistry (IHC) staining from The Human Protein Atlas (THPA) database.

    Immunohistochemical staining:

    Article Title: C1orf226 Promotes Glioma Stemness and Oncogenesis in Glioma via PLK1-Mediated Wnt/β-Catenin Signaling.
    Article Snippet: .. Images of immunohistochemical (IHC) staining to validate C1orf226 protein expression were obtained from the Human Protein Atlas (HPA) [21]. ..



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    Bilayer hydrogel orchestrates inflammatory cell dynamics during the early inflammation phase of diabetic wound healing. a Experimental timeline for assay of early neutrophil recruitment. b Immunohistochemical staining for Ly-6G in wounds at 8 h, 1 d and 3 d after injury. Diabetic wounds were treated with SP/IL-10@Bilayer, SP@Bilayer, IL-10@Bilayer, and saline solution (Model), respectively. Healthy mice treated with saline solution were set as Normal. c Quantitative analysis of Ly-6G + cells in each group. d Relative expression of CXCL-1 on day 1. e Relative expression of MCP-1 on day 1. f Experimental timeline for assay of M1 macrophage infiltration. g <t>Immunofluorescence</t> staining for iNOS in wounds on days 1, 3 and 6 after injury. h Quantitative analysis of iNOS + cells in each group. i-k Relative expressions of macrophage-associated pro-inflammatory cytokines including TNF-α, IL-1β and IL-6 on day 3. l Schematic illustrating the dynamic modulation of inflammatory cells during the early inflammation phase of diabetic wounds by SP/IL-10@Bilayer. All data were generated from at least three independent experiments and presented as the means ± standard deviation. Statistical analysis was performed by one-way ANOVA. # means significant difference compared to the normal group. #p < 0.05, ##p < 0.01 and ###p < 0.001; ∗ means significant difference compared to the model group. ∗p < 0.05; & means significant difference compared to SP/IL-10@Bilayer. & p < 0.05 and && p < 0.01.
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    Image Search Results


    Bilayer hydrogel orchestrates inflammatory cell dynamics during the early inflammation phase of diabetic wound healing. a Experimental timeline for assay of early neutrophil recruitment. b Immunohistochemical staining for Ly-6G in wounds at 8 h, 1 d and 3 d after injury. Diabetic wounds were treated with SP/IL-10@Bilayer, SP@Bilayer, IL-10@Bilayer, and saline solution (Model), respectively. Healthy mice treated with saline solution were set as Normal. c Quantitative analysis of Ly-6G + cells in each group. d Relative expression of CXCL-1 on day 1. e Relative expression of MCP-1 on day 1. f Experimental timeline for assay of M1 macrophage infiltration. g Immunofluorescence staining for iNOS in wounds on days 1, 3 and 6 after injury. h Quantitative analysis of iNOS + cells in each group. i-k Relative expressions of macrophage-associated pro-inflammatory cytokines including TNF-α, IL-1β and IL-6 on day 3. l Schematic illustrating the dynamic modulation of inflammatory cells during the early inflammation phase of diabetic wounds by SP/IL-10@Bilayer. All data were generated from at least three independent experiments and presented as the means ± standard deviation. Statistical analysis was performed by one-way ANOVA. # means significant difference compared to the normal group. #p < 0.05, ##p < 0.01 and ###p < 0.001; ∗ means significant difference compared to the model group. ∗p < 0.05; & means significant difference compared to SP/IL-10@Bilayer. & p < 0.05 and && p < 0.01.

    Journal: Bioactive Materials

    Article Title: Skin-mimetic bilayer hydrogel normalizes diabetic wound healing by orchestrating inflammatory cell dynamics: An early intervention strategy

    doi: 10.1016/j.bioactmat.2026.02.025

    Figure Lengend Snippet: Bilayer hydrogel orchestrates inflammatory cell dynamics during the early inflammation phase of diabetic wound healing. a Experimental timeline for assay of early neutrophil recruitment. b Immunohistochemical staining for Ly-6G in wounds at 8 h, 1 d and 3 d after injury. Diabetic wounds were treated with SP/IL-10@Bilayer, SP@Bilayer, IL-10@Bilayer, and saline solution (Model), respectively. Healthy mice treated with saline solution were set as Normal. c Quantitative analysis of Ly-6G + cells in each group. d Relative expression of CXCL-1 on day 1. e Relative expression of MCP-1 on day 1. f Experimental timeline for assay of M1 macrophage infiltration. g Immunofluorescence staining for iNOS in wounds on days 1, 3 and 6 after injury. h Quantitative analysis of iNOS + cells in each group. i-k Relative expressions of macrophage-associated pro-inflammatory cytokines including TNF-α, IL-1β and IL-6 on day 3. l Schematic illustrating the dynamic modulation of inflammatory cells during the early inflammation phase of diabetic wounds by SP/IL-10@Bilayer. All data were generated from at least three independent experiments and presented as the means ± standard deviation. Statistical analysis was performed by one-way ANOVA. # means significant difference compared to the normal group. #p < 0.05, ##p < 0.01 and ###p < 0.001; ∗ means significant difference compared to the model group. ∗p < 0.05; & means significant difference compared to SP/IL-10@Bilayer. & p < 0.05 and && p < 0.01.

    Article Snippet: The infiltration of pro-inflammatory (M1) macrophages and polarization of M2c macrophages were analyzed by immunofluorescence staining using antibodies against iNOS (Servicebio, GB11119) and CD163 (Servicebio, GB14027), respectively.

    Techniques: Immunohistochemical staining, Staining, Saline, Expressing, Immunofluorescence, Generated, Standard Deviation

    Bilayer hydrogel modulates M2c macrophage polarization in the later healing phase of diabetic wound. a Experimental timeline for assay of M2c macrophage polarization. b Immunofluorescence staining for CD163 in wounds on days 3, 6, 9 and 12 after injury. c Quantitative analysis of CD163 + cells in each group. d-f Relative expression of MerTK, IL-10, and TGF-β1 on day 6. g Schematic illustration of M2c macrophage polarization regulated by SP/IL-10@Bilayer and its contribution to inflammation resolution. All data were generated from three independent experiments and presented as the means ± standard deviation. Statistical analysis was performed by one-way ANOVA. # means significant difference compared to the normal group. #p < 0.05 and ##p < 0.01; ∗ means significant difference compared to model group. ∗p < 0.05, ∗∗p < 0.01 and ∗∗∗p < 0.001; & means significant difference compared to SP/IL-10@Bilayer group. & p < 0.05.

    Journal: Bioactive Materials

    Article Title: Skin-mimetic bilayer hydrogel normalizes diabetic wound healing by orchestrating inflammatory cell dynamics: An early intervention strategy

    doi: 10.1016/j.bioactmat.2026.02.025

    Figure Lengend Snippet: Bilayer hydrogel modulates M2c macrophage polarization in the later healing phase of diabetic wound. a Experimental timeline for assay of M2c macrophage polarization. b Immunofluorescence staining for CD163 in wounds on days 3, 6, 9 and 12 after injury. c Quantitative analysis of CD163 + cells in each group. d-f Relative expression of MerTK, IL-10, and TGF-β1 on day 6. g Schematic illustration of M2c macrophage polarization regulated by SP/IL-10@Bilayer and its contribution to inflammation resolution. All data were generated from three independent experiments and presented as the means ± standard deviation. Statistical analysis was performed by one-way ANOVA. # means significant difference compared to the normal group. #p < 0.05 and ##p < 0.01; ∗ means significant difference compared to model group. ∗p < 0.05, ∗∗p < 0.01 and ∗∗∗p < 0.001; & means significant difference compared to SP/IL-10@Bilayer group. & p < 0.05.

    Article Snippet: The infiltration of pro-inflammatory (M1) macrophages and polarization of M2c macrophages were analyzed by immunofluorescence staining using antibodies against iNOS (Servicebio, GB11119) and CD163 (Servicebio, GB14027), respectively.

    Techniques: Immunofluorescence, Staining, Expressing, Generated, Standard Deviation