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ice-cold co-ip lysis buffer  (Beyotime)


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    Structured Review

    Beyotime ice-cold co-ip lysis buffer
    Ice Cold Co Ip Lysis Buffer, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ice+cold+co+ip+lysis+buffer/lysis+buffer/pmc07293984-73-6-10
    Average 90 stars, based on 1 article reviews
    ice-cold co-ip lysis buffer - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    other:

    Article Title: miR-30a enhanced RIG-I-mediated type I interferon antiviral response by targeting USP14.
    Article Snippet: Cells were lysed with RIPA or NP-40 lysis buffer (Beyotime) supplemented with a protease inhibitor cocktail (VICMED, China).

    Article Title: Phosphoglycerate dehydrogenase stabilizes protein kinase C delta type mRNA to promote hepatocellular carcinoma progression
    Article Snippet: The collected cells were washed with cold PBS and then lysed at 4 °C for 30 min with cold NP-40 lysis buffer (Beyotime Biotechnology, China) supplemented with 1×phosphatase inhibitor cocktail and 1× protease inhibitor.

    Article Title: Crizotinib: A Novel Strategy to Reverse Immunosuppression in Melanoma by Targeting Lactate Transport
    Article Snippet: Subsequently, cell lysis was performed at 4°C using 0.5% NP‐40 lysis buffer (Beyotime, China).

    Article Title: Salvianolic Acid B Attenuates Ferroptosis in Acute Kidney Injury by Targeting PRDX5
    Article Snippet: In brief, approximately 1 × 10 7 HK‐2 cells were lysed on ice for 30 min using NP‐40 extraction reagent (Beyotime Biotechnology, Jiangsu, China).

    Lysis:

    Article Title: SLC35F2 promotes the progression of NSCLC via regulating CREB1 expression
    Article Snippet: .. Brifly, collect the lyse of cells in different groups using a lysis buffer (Beyotime Technology, Shanghai, China) containing protease inhibitors to prevent protein degradation. ..

    Article Title: Topical administration of GLP-1 eyedrops improves retinal ganglion cell function by facilitating presynaptic GABA release in early experimental diabetes
    Article Snippet: Retinas, vitreous humor, ciliary bodies, and sclerae were harvested 1 or 2 hours after GLP-1 eyedrop administration for enzyme-linked immunosorbent assays (ELISAs) to measure GLP-1 penetration in ocular tissues. .. The isolated tissues were promptly frozen in liquid nitrogen and sonicated in lysis buffer (1:10 dilution; Beyotime, Shanghai, China) supplemented with a 1:200 dilution of protease inhibitor cocktail (P8340; Sigma-Aldrich). .. GLP-1 concentrations were quantified using the GLP-1 Multispecies ELISA Kit (BMS2194; Invitrogen, Carlsbad, CA, USA) following the manufacturer’s instructions.

    Article Title: MIR4726 EccDNA drives bortezomib resistance in multiple myeloma by enhancing MIR4726-5p/NXF1/NKIRAS2 axis dependent autophagy
    Article Snippet: .. The cells were harvested and lysed in cell lysis buffer (Beyotime, Shanghai, China) containing 10 nmol/L protease inhibitor and 10 nmol/L phosphatase inhibitor for western blotting. .. An Omni-EasyTM Ready-to-use BCA Protein Assay Kit (Epizyme, Shanghai, China) was used for protein quantification.

    Isolation:

    Article Title: Topical administration of GLP-1 eyedrops improves retinal ganglion cell function by facilitating presynaptic GABA release in early experimental diabetes
    Article Snippet: Retinas, vitreous humor, ciliary bodies, and sclerae were harvested 1 or 2 hours after GLP-1 eyedrop administration for enzyme-linked immunosorbent assays (ELISAs) to measure GLP-1 penetration in ocular tissues. .. The isolated tissues were promptly frozen in liquid nitrogen and sonicated in lysis buffer (1:10 dilution; Beyotime, Shanghai, China) supplemented with a 1:200 dilution of protease inhibitor cocktail (P8340; Sigma-Aldrich). .. GLP-1 concentrations were quantified using the GLP-1 Multispecies ELISA Kit (BMS2194; Invitrogen, Carlsbad, CA, USA) following the manufacturer’s instructions.

    Sonication:

    Article Title: Topical administration of GLP-1 eyedrops improves retinal ganglion cell function by facilitating presynaptic GABA release in early experimental diabetes
    Article Snippet: Retinas, vitreous humor, ciliary bodies, and sclerae were harvested 1 or 2 hours after GLP-1 eyedrop administration for enzyme-linked immunosorbent assays (ELISAs) to measure GLP-1 penetration in ocular tissues. .. The isolated tissues were promptly frozen in liquid nitrogen and sonicated in lysis buffer (1:10 dilution; Beyotime, Shanghai, China) supplemented with a 1:200 dilution of protease inhibitor cocktail (P8340; Sigma-Aldrich). .. GLP-1 concentrations were quantified using the GLP-1 Multispecies ELISA Kit (BMS2194; Invitrogen, Carlsbad, CA, USA) following the manufacturer’s instructions.

    Protease Inhibitor:

    Article Title: Topical administration of GLP-1 eyedrops improves retinal ganglion cell function by facilitating presynaptic GABA release in early experimental diabetes
    Article Snippet: Retinas, vitreous humor, ciliary bodies, and sclerae were harvested 1 or 2 hours after GLP-1 eyedrop administration for enzyme-linked immunosorbent assays (ELISAs) to measure GLP-1 penetration in ocular tissues. .. The isolated tissues were promptly frozen in liquid nitrogen and sonicated in lysis buffer (1:10 dilution; Beyotime, Shanghai, China) supplemented with a 1:200 dilution of protease inhibitor cocktail (P8340; Sigma-Aldrich). .. GLP-1 concentrations were quantified using the GLP-1 Multispecies ELISA Kit (BMS2194; Invitrogen, Carlsbad, CA, USA) following the manufacturer’s instructions.

    Article Title: MIR4726 EccDNA drives bortezomib resistance in multiple myeloma by enhancing MIR4726-5p/NXF1/NKIRAS2 axis dependent autophagy
    Article Snippet: .. The cells were harvested and lysed in cell lysis buffer (Beyotime, Shanghai, China) containing 10 nmol/L protease inhibitor and 10 nmol/L phosphatase inhibitor for western blotting. .. An Omni-EasyTM Ready-to-use BCA Protein Assay Kit (Epizyme, Shanghai, China) was used for protein quantification.

    Article Title: NSD1-916aa encoded by CircNSD1 contributes to AKI-to-CKD transition through inducing ferroptosis in tubular epithelial cells.
    Article Snippet: Luciferase activity was measured using the Dual-Glo Luciferase Assay Kit (Promega,405 Madison, WI, USA) to quantify firefly and Renilla luciferase activities, following the manufacturer’s406 protocol (42, 43).407 Immunoprecipitation408 HK-2 Cells were harvested and washed thrice with ice-cold PBS as previously described (44). .. The409 cells were lysed in IP buffer (Beyotime Biotechnology, Shanghai, China) supplemented with a410 complete protease inhibitor cocktail (Yeasen, Shanghai, China) on ice for 30 min. Lysates were411 collected, and immunoprecipitation was performed using anti-Flag Nanoab Magarose beads412 (NuoyiBio, Tianjin) by incubating with protein samples at 4°C for 12 hours. ..

    Western Blot:

    Article Title: MIR4726 EccDNA drives bortezomib resistance in multiple myeloma by enhancing MIR4726-5p/NXF1/NKIRAS2 axis dependent autophagy
    Article Snippet: .. The cells were harvested and lysed in cell lysis buffer (Beyotime, Shanghai, China) containing 10 nmol/L protease inhibitor and 10 nmol/L phosphatase inhibitor for western blotting. .. An Omni-EasyTM Ready-to-use BCA Protein Assay Kit (Epizyme, Shanghai, China) was used for protein quantification.

    Immunoprecipitation:

    Article Title: NSD1-916aa encoded by CircNSD1 contributes to AKI-to-CKD transition through inducing ferroptosis in tubular epithelial cells.
    Article Snippet: Luciferase activity was measured using the Dual-Glo Luciferase Assay Kit (Promega,405 Madison, WI, USA) to quantify firefly and Renilla luciferase activities, following the manufacturer’s406 protocol (42, 43).407 Immunoprecipitation408 HK-2 Cells were harvested and washed thrice with ice-cold PBS as previously described (44). .. The409 cells were lysed in IP buffer (Beyotime Biotechnology, Shanghai, China) supplemented with a410 complete protease inhibitor cocktail (Yeasen, Shanghai, China) on ice for 30 min. Lysates were411 collected, and immunoprecipitation was performed using anti-Flag Nanoab Magarose beads412 (NuoyiBio, Tianjin) by incubating with protein samples at 4°C for 12 hours. ..



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