alkaline phosphatase  (MedChemExpress)

Journal: Genes

Article Title: Regulation of Smad2/3 Nuclear Exclusion by Follicle-Stimulating Hormone (FSH) in Chicken Follicular Granulosa Cells and Its Effect on FOXO3/4

doi: 10.3390/genes16030283

Figure Lengend Snippet: The nuclear exclusion of Smad2/3 in the cultured granulosa cells (GCs) of the ovarian follicles. ( A ) Immunofluorescence detection of Smad2 subcellular localization in the GCs. Smad2 is shown in green, and nucleus is stained with 4′,6-diamidino-2-phenylindole (DAPI) (blue). ( B ) Immunofluorescence detection of Smad3 subcellular localization in the GCs. Smad3 is shown in green, and nucleus is stained with DAPI (blue). a. control, without any treatment; b. treatment of the cells with 10 ng/mL follicle-stimulating hormone (FSH) for 12 h; c. co-treatment of the cells with 10 ng/mL FSH and phosphatidylinositol 3-kinase (PI3K) inhibitor for 12 h. d. control, without any treatment; e. treatment of the cells with 50 μg/mL PI3K activator for 12 h; f. co-treatment of the cells with 50 μg/mL PI3K activator and 50 μg/mL alkaline phosphatase for 12 h. n = 3 independent experiments. Leica DMI8, 400×; scale bar = 5 μm.

Article Snippet: The cells were cultured with 10 ng/mL FSH and 50 μg/mL PI3K activator (Selleck Chemicals, Houston, TX, USA) for 12 h in the presence or absence of 16 nM/mL PI3K inhibitor (Med Chem Express, Monmouth Junction, NJ, USA) and 50 μg/mL alkaline phosphatase (Yuanye, Beijing, China) [ , ].

Techniques: Cell Culture, Immunofluorescence, Staining, Control

Journal: Genes

Article Title: Regulation of Smad2/3 Nuclear Exclusion by Follicle-Stimulating Hormone (FSH) in Chicken Follicular Granulosa Cells and Its Effect on FOXO3/4

doi: 10.3390/genes16030283

Figure Lengend Snippet: Smad2/3 phosphorylation in the cultured GCs of the ovarian follicles. ( A ) The Smad2 and p-Smad2 levels were determined by Western blotting analysis in the GCs without any treatment, treated with 10 ng/mL FSH for 12 h, and co-treated with 10 ng/mL FSH and 16 nM/mL PI3K inhibitor for 12 h. ( B ) The Smad3 and p-Smad3 levels were tested in the GCs without any treatment, treated with 10 ng/mL FSH for 12 h, and co-treated with 10 ng/mL FSH and 16 nM/mL PI3K inhibitor for 12 h. ( C ) The Smad2 and p-Smad2 levels were determined by Western blotting analysis in the GCs without any treatment, treated with 50 μg/mL PI3K activator for 12 h, and co-treated with 50 μg/mL PI3K activator and 50 μg/mL alkaline phosphatase for 12 h. ( D ) The Smad3 and p-Smad3 levels were tested in the GCs under the following conditions: without any treatment, treated with 50 μg/mL PI3K activator for 12 h, and co-treated with 50 μg/mL PI3K activator and 50 μg/mL alkaline phosphatase for 12 h. The data represent the mean ± SEM ( n = 3). Student’s t -test, ** p < 0.01 and * p < 0.05.

Article Snippet: The cells were cultured with 10 ng/mL FSH and 50 μg/mL PI3K activator (Selleck Chemicals, Houston, TX, USA) for 12 h in the presence or absence of 16 nM/mL PI3K inhibitor (Med Chem Express, Monmouth Junction, NJ, USA) and 50 μg/mL alkaline phosphatase (Yuanye, Beijing, China) [ , ].

Techniques: Cell Culture, Western Blot