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horse serum  (MedChemExpress)


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    Structured Review

    MedChemExpress horse serum
    Horse Serum, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hy-p2798/Cholinesterase%2C+Horse+serum/pm40593645-326-27-45
    Average 94 stars, based on 7 article reviews
    horse serum - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Engineering a controllable and reversible switch for CAR-based cellular immunotherapies via a genetic code expansion system
    Article Snippet: Jurkat T cells and Nalm6-luciferase (Nalm6-Luc) cells were cultured in RPMI-1640 (BI, Israel) supplemented with 10% FBS (BI, Israel). .. NK92 cells were cultured in MEMα supplemented with 0.2 mM inositol, 0.1 mM β-mercaptoethanol, 0.02 mM folic acid, 12.5% horse serum, and 12.5% FBS (Pricella Life Science & Technology, China) with 200 U of IL-2 (MCE, USA). .. All cell lines were maintained at 37 °C in a cell culture incubator (Thermo Fisher, USA) with a humidified 5% CO 2 atmosphere.

    Article Title: Engineering a controllable and reversible switch for CAR-based cellular immunotherapies via a genetic code expansion system.
    Article Snippet: Jurkat T cells and Nalm6-luciferase (Nalm6-Luc) cells were cultured in RPMI-1640 (BI, Israel) supplemented with 10% FBS (BI, Israel). .. NK92 cells were cultured in MEMα supplemented with 0.2 mM inositol, 0.1 mM β-mercaptoethanol, 0.02 mM folic acid, 12.5% horse serum, and 12.5% FBS (Pricella Life Science & Technology, China) with 200 U of IL-2 (MCE, USA). .. All cell lines were maintained at 37 °C in a cell culture incubator (Thermo Fisher, USA) with a humidified 5% CO2 atmosphere.

    Formulation:

    Article Title: Perturbation of mammary epithelial cell apicobasal polarity by RHBDF1-facilitated nuclear translocation of PKCζ
    Article Snippet: MCF10A cells were purchased from American Type Culture Collection (ATCC, Rockefeller, USA), and cultured in the culture media with 5% CO 2 at 37 °C. .. The culture media formulation is as follows: 500 mL DMEM/F12 (Invitrogen, Carlsbad, USA), containing 5% (v/v) Horse serum (Solarbio, Beijing, China), 20 ng/mL of Recombinant Human EGF (PeproTech, Rocky Hill, USA), 0.5 μg/mL, 100 ng/mL and 10 μg/mL of Hydrocortisone, Cholera toxin and Insulin, respectively (MedChemExpress, New Jersey, USA), and 1% penicillin (50 U/mL)-streptomycin (50 μg/mL) (Invitrogen, Carlsbad, USA). .. The lentivirus: pLV[Exp]-EGFP: T2A: Puro-Null, pLV[Exp]-EGFP: T2A: Puro-EF1A > HA/hRHBDF1 and pLV[TetOn]-EGFP: T2A: Puro-TRE > HA/hRHBDF1 used to infect MCF10A cells were purchased from VectorBuilder (Guangzhou, China).

    Article Title: Perturbation of mammary epithelial cell apicobasal polarity by RHBDF1-facilitated nuclear translocation of PKCζ.
    Article Snippet: Cell culture and establishment of stable overexpressed RHBDF1 MCF-10 A cell lines MCF10A cells were purchased from American Type Culture Collection (ATCC, Rockefeller, USA), and cultured in the culture media with 5% CO2 at 37 °C. .. The culture media formulation is as follows: 500 mL DMEM/F12 (Invitrogen, Carlsbad, USA), containing 5% (v/v) Horse serum (Solarbio, Beijing, China), 20 ng/mL of Recombinant Human EGF (PeproTech, Rocky Hill, USA), 0.5 μg/ mL, 100 ng/mL and 10 μg/mL of Hydrocortisone, Cholera toxin and Insulin, respectively (MedChemExpress, New Jersey, USA), and 1% penicillin (50 U/mL)-streptomycin (50 μg/mL) (Invitrogen, Carlsbad, USA). .. The lentivirus: pLV[Exp]-EGFP: T2A: Puro-Null, pLV[Exp]-EGFP: T2A: Puro-EF1A > HA/hRHBDF1 and pLV[TetOn]-EGFP: T2A: Puro-TRE > HA/hRHBDF1 used to infect MCF10A cells were purchased from VectorBuilder (Guangzhou, China).

    Recombinant:

    Article Title: Perturbation of mammary epithelial cell apicobasal polarity by RHBDF1-facilitated nuclear translocation of PKCζ
    Article Snippet: MCF10A cells were purchased from American Type Culture Collection (ATCC, Rockefeller, USA), and cultured in the culture media with 5% CO 2 at 37 °C. .. The culture media formulation is as follows: 500 mL DMEM/F12 (Invitrogen, Carlsbad, USA), containing 5% (v/v) Horse serum (Solarbio, Beijing, China), 20 ng/mL of Recombinant Human EGF (PeproTech, Rocky Hill, USA), 0.5 μg/mL, 100 ng/mL and 10 μg/mL of Hydrocortisone, Cholera toxin and Insulin, respectively (MedChemExpress, New Jersey, USA), and 1% penicillin (50 U/mL)-streptomycin (50 μg/mL) (Invitrogen, Carlsbad, USA). .. The lentivirus: pLV[Exp]-EGFP: T2A: Puro-Null, pLV[Exp]-EGFP: T2A: Puro-EF1A > HA/hRHBDF1 and pLV[TetOn]-EGFP: T2A: Puro-TRE > HA/hRHBDF1 used to infect MCF10A cells were purchased from VectorBuilder (Guangzhou, China).

    Article Title: Perturbation of mammary epithelial cell apicobasal polarity by RHBDF1-facilitated nuclear translocation of PKCζ.
    Article Snippet: Cell culture and establishment of stable overexpressed RHBDF1 MCF-10 A cell lines MCF10A cells were purchased from American Type Culture Collection (ATCC, Rockefeller, USA), and cultured in the culture media with 5% CO2 at 37 °C. .. The culture media formulation is as follows: 500 mL DMEM/F12 (Invitrogen, Carlsbad, USA), containing 5% (v/v) Horse serum (Solarbio, Beijing, China), 20 ng/mL of Recombinant Human EGF (PeproTech, Rocky Hill, USA), 0.5 μg/ mL, 100 ng/mL and 10 μg/mL of Hydrocortisone, Cholera toxin and Insulin, respectively (MedChemExpress, New Jersey, USA), and 1% penicillin (50 U/mL)-streptomycin (50 μg/mL) (Invitrogen, Carlsbad, USA). .. The lentivirus: pLV[Exp]-EGFP: T2A: Puro-Null, pLV[Exp]-EGFP: T2A: Puro-EF1A > HA/hRHBDF1 and pLV[TetOn]-EGFP: T2A: Puro-TRE > HA/hRHBDF1 used to infect MCF10A cells were purchased from VectorBuilder (Guangzhou, China).

    Article Title: Molecular mechanism of Activin receptor inhibition by DLK1
    Article Snippet: The protocol for PLA was then followed according to the manufacturer’s specifications (Invitrogen) using primary antibodies Notch1 A-8 (Santa Cruz Biotechnology, Cat# sc-376403) and SMAD2/3 (Cell Signaling, Cat# 8685) at 1:100 concentration. .. For C2C12 differentiation assays, cells were plated in 24 well plates (Cellvis) and at ~70% confluency the experiment was started (Day 0) by replacing growth media with DMEM + 2% horse serum (differentiation media) and blocked for 30 min with recombinant soluble DLK1, DLK1 R193D -mutant or ACVR2B blocking antibody (Bimagrumab, MedChemExpress, Cat# HY-P99355) (100 nM) before addition of 4 μg/ml recombinant active myostatin (R&D systems). ..

    Article Title: Molecular mechanism of Activin receptor inhibition by DLK1.
    Article Snippet: The protocol for PLA was then followed according to the manufacturer’s specifications (Invitrogen) using primary antibodies Notch1 A-8 (Santa Cruz Biotechnology, Cat# sc-376403) and SMAD2/3 (Cell Signaling, Cat# 8685) at 1:100 concentration. .. For C2C12 differentiation assays, cells were plated in 24well plates (Cellvis) and at ~70%confluency the experiment was started (Day 0) by replacing growth media with DMEM+ 2% horse serum (differentiation media) and blocked for 30min with recombinant soluble DLK1, DLK1R193D-mutant or ACVR2B blocking antibody (Bimagrumab, MedChemExpress, Cat# HY-P99355) (100 nM) before addition of 4μg/ml recombinant active myostatin (R&D systems). ..

    Article Title: Optic atrophy 1 mediates muscle differentiation by promoting a metabolic switch via the supercomplex assembly factor SCAF1.
    Article Snippet: Horseradish peroxidase-conjugated Donkey anti-rabbit (H+L) Jackson ImmunoResearch Cat# A16023; RRID:AB_2534697 .. Chemicals, peptides, and recombinant proteins Dulbecco’s Modified Eagle’s Medium Wisent Cat# 319-005-CL HAMS F-10 Medium Wisent Cat# 318-050-CL Phosphate buffered saline Wisent Cat# 311-010-CL TrypLE Express Gibco Cat# 12605010 Penicillin-Streptomycin Wisent Cat# Wisent, 450-201-EL Fetal Bovine Serum Wisent Cat# 080-150 Horse Serum Wisent Cat# 065-150 bFGF Peprotech Cat# 100-18B MYLS22 Med Chem Express Ca# HY-136446 Bovine serum albumin Sigma Cat# A4503 Triton x100 Sigma Cat# 1002560820 ..

    Mutagenesis:

    Article Title: Molecular mechanism of Activin receptor inhibition by DLK1
    Article Snippet: The protocol for PLA was then followed according to the manufacturer’s specifications (Invitrogen) using primary antibodies Notch1 A-8 (Santa Cruz Biotechnology, Cat# sc-376403) and SMAD2/3 (Cell Signaling, Cat# 8685) at 1:100 concentration. .. For C2C12 differentiation assays, cells were plated in 24 well plates (Cellvis) and at ~70% confluency the experiment was started (Day 0) by replacing growth media with DMEM + 2% horse serum (differentiation media) and blocked for 30 min with recombinant soluble DLK1, DLK1 R193D -mutant or ACVR2B blocking antibody (Bimagrumab, MedChemExpress, Cat# HY-P99355) (100 nM) before addition of 4 μg/ml recombinant active myostatin (R&D systems). ..

    Blocking Assay:

    Article Title: Molecular mechanism of Activin receptor inhibition by DLK1
    Article Snippet: The protocol for PLA was then followed according to the manufacturer’s specifications (Invitrogen) using primary antibodies Notch1 A-8 (Santa Cruz Biotechnology, Cat# sc-376403) and SMAD2/3 (Cell Signaling, Cat# 8685) at 1:100 concentration. .. For C2C12 differentiation assays, cells were plated in 24 well plates (Cellvis) and at ~70% confluency the experiment was started (Day 0) by replacing growth media with DMEM + 2% horse serum (differentiation media) and blocked for 30 min with recombinant soluble DLK1, DLK1 R193D -mutant or ACVR2B blocking antibody (Bimagrumab, MedChemExpress, Cat# HY-P99355) (100 nM) before addition of 4 μg/ml recombinant active myostatin (R&D systems). ..

    Article Title: Molecular mechanism of Activin receptor inhibition by DLK1.
    Article Snippet: The protocol for PLA was then followed according to the manufacturer’s specifications (Invitrogen) using primary antibodies Notch1 A-8 (Santa Cruz Biotechnology, Cat# sc-376403) and SMAD2/3 (Cell Signaling, Cat# 8685) at 1:100 concentration. .. For C2C12 differentiation assays, cells were plated in 24well plates (Cellvis) and at ~70%confluency the experiment was started (Day 0) by replacing growth media with DMEM+ 2% horse serum (differentiation media) and blocked for 30min with recombinant soluble DLK1, DLK1R193D-mutant or ACVR2B blocking antibody (Bimagrumab, MedChemExpress, Cat# HY-P99355) (100 nM) before addition of 4μg/ml recombinant active myostatin (R&D systems). ..

    Modification:

    Article Title: Optic atrophy 1 mediates muscle differentiation by promoting a metabolic switch via the supercomplex assembly factor SCAF1.
    Article Snippet: Horseradish peroxidase-conjugated Donkey anti-rabbit (H+L) Jackson ImmunoResearch Cat# A16023; RRID:AB_2534697 .. Chemicals, peptides, and recombinant proteins Dulbecco’s Modified Eagle’s Medium Wisent Cat# 319-005-CL HAMS F-10 Medium Wisent Cat# 318-050-CL Phosphate buffered saline Wisent Cat# 311-010-CL TrypLE Express Gibco Cat# 12605010 Penicillin-Streptomycin Wisent Cat# Wisent, 450-201-EL Fetal Bovine Serum Wisent Cat# 080-150 Horse Serum Wisent Cat# 065-150 bFGF Peprotech Cat# 100-18B MYLS22 Med Chem Express Ca# HY-136446 Bovine serum albumin Sigma Cat# A4503 Triton x100 Sigma Cat# 1002560820 ..

    Saline:

    Article Title: Optic atrophy 1 mediates muscle differentiation by promoting a metabolic switch via the supercomplex assembly factor SCAF1.
    Article Snippet: Horseradish peroxidase-conjugated Donkey anti-rabbit (H+L) Jackson ImmunoResearch Cat# A16023; RRID:AB_2534697 .. Chemicals, peptides, and recombinant proteins Dulbecco’s Modified Eagle’s Medium Wisent Cat# 319-005-CL HAMS F-10 Medium Wisent Cat# 318-050-CL Phosphate buffered saline Wisent Cat# 311-010-CL TrypLE Express Gibco Cat# 12605010 Penicillin-Streptomycin Wisent Cat# Wisent, 450-201-EL Fetal Bovine Serum Wisent Cat# 080-150 Horse Serum Wisent Cat# 065-150 bFGF Peprotech Cat# 100-18B MYLS22 Med Chem Express Ca# HY-136446 Bovine serum albumin Sigma Cat# A4503 Triton x100 Sigma Cat# 1002560820 ..



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