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opn integrin ανβ 3  (MedChemExpress)


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    Structured Review

    MedChemExpress opn integrin ανβ 3
    Opn Integrin ανβ 3, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hy-p2532/Integrin+Binding+Peptide/pmc07593734-73-43-60
    Average 90 stars, based on 1 article reviews
    opn integrin ανβ 3 - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Expressing:

    Article Title: Mechanistic Insight on the Interaction between OPN and Integrin ανβ 3 in Osteoarthritis
    Article Snippet: The cells were incubated in a 5% CO 2 incubator at 37°C for 2 days and then take them out for experimentation; OA-NC group—OA chondrocytes were transfected with plasmid without silencing sequence as control, and the specific method is the same as the si-OPN group; rhOPN group—1 μ g/ml recombinant human osteopontin (rhOPN) (Genechem, Shanghai, China) was added to the OA chondrocyte culture medium to upregulate the expression of OPN in the culture system. .. After intervention for 24 hours, the expression of related proteins and RNA was detected; ανβ 3 Ab+rhOPN group—pretreated with anti-integrin ανβ 3 blocking mAb (100 μ g/ml) (593274-97-6, Baileibo, Beijing, China) to interfere with chondrocytes for 1 h to block the binding of OPN-integrin ανβ 3 and then add 1 μ g/ml rhOPN intervention for 23 h; GRGDSP+rhOPN group—GRGDSP (91037-75-1, MCE, China) peptide (100 μ g/ml) was used to block the binding of OPN to integrin subtype receptors for 1 h, and then 1 μ g/ml rhOPN was added for 23 h. .. As previously described [ , ], total RNA from chondrocytes and cartilage tissues was extracted using TRIzol (CW Biotech, Beijing, China).

    Blocking Assay:

    Article Title: Mechanistic Insight on the Interaction between OPN and Integrin ανβ 3 in Osteoarthritis
    Article Snippet: The cells were incubated in a 5% CO 2 incubator at 37°C for 2 days and then take them out for experimentation; OA-NC group—OA chondrocytes were transfected with plasmid without silencing sequence as control, and the specific method is the same as the si-OPN group; rhOPN group—1 μ g/ml recombinant human osteopontin (rhOPN) (Genechem, Shanghai, China) was added to the OA chondrocyte culture medium to upregulate the expression of OPN in the culture system. .. After intervention for 24 hours, the expression of related proteins and RNA was detected; ανβ 3 Ab+rhOPN group—pretreated with anti-integrin ανβ 3 blocking mAb (100 μ g/ml) (593274-97-6, Baileibo, Beijing, China) to interfere with chondrocytes for 1 h to block the binding of OPN-integrin ανβ 3 and then add 1 μ g/ml rhOPN intervention for 23 h; GRGDSP+rhOPN group—GRGDSP (91037-75-1, MCE, China) peptide (100 μ g/ml) was used to block the binding of OPN to integrin subtype receptors for 1 h, and then 1 μ g/ml rhOPN was added for 23 h. .. As previously described [ , ], total RNA from chondrocytes and cartilage tissues was extracted using TRIzol (CW Biotech, Beijing, China).

    Binding Assay:

    Article Title: Mechanistic Insight on the Interaction between OPN and Integrin ανβ 3 in Osteoarthritis
    Article Snippet: The cells were incubated in a 5% CO 2 incubator at 37°C for 2 days and then take them out for experimentation; OA-NC group—OA chondrocytes were transfected with plasmid without silencing sequence as control, and the specific method is the same as the si-OPN group; rhOPN group—1 μ g/ml recombinant human osteopontin (rhOPN) (Genechem, Shanghai, China) was added to the OA chondrocyte culture medium to upregulate the expression of OPN in the culture system. .. After intervention for 24 hours, the expression of related proteins and RNA was detected; ανβ 3 Ab+rhOPN group—pretreated with anti-integrin ανβ 3 blocking mAb (100 μ g/ml) (593274-97-6, Baileibo, Beijing, China) to interfere with chondrocytes for 1 h to block the binding of OPN-integrin ανβ 3 and then add 1 μ g/ml rhOPN intervention for 23 h; GRGDSP+rhOPN group—GRGDSP (91037-75-1, MCE, China) peptide (100 μ g/ml) was used to block the binding of OPN to integrin subtype receptors for 1 h, and then 1 μ g/ml rhOPN was added for 23 h. .. As previously described [ , ], total RNA from chondrocytes and cartilage tissues was extracted using TRIzol (CW Biotech, Beijing, China).



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    90
    MedChemExpress opn integrin ανβ 3
    Opn Integrin ανβ 3, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hy-p2532/Integrin+Binding+Peptide/pmc07593734-73-43-60
    Average 90 stars, based on 1 article reviews
    opn integrin ανβ 3 - by Bioz Stars, 2026-10
    90/100 stars
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