Review



nattokinase  (MedChemExpress)


Bioz Verified Symbol MedChemExpress is a verified supplier
Bioz Manufacturer Symbol MedChemExpress manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    MedChemExpress nattokinase
    Nattokinase, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hy-p2373/Nattokinase/10__1016_slash_j__jds__2025__10__022-34-5-27
    Average 94 stars, based on 6 article reviews
    nattokinase - by Bioz Stars, 2026-10
    94/100 stars

    Images

    Related Articles

    Single Cell:

    Article Title: Replicating Dynamic Immune Responses at Single-Cell Resolution within a Microfluidic Human Skin Equivalent.
    Article Snippet: .. For single-cell RNA sequencing (scRNA-seq), the samples were removed from the frames and digested with 300 μL of 50 FU mL−1 nattokinase (MedChem Express) dissolved in sterile DPBS for 1 h at 37 °C 5% CO2. ..

    Article Title: Replicating dynamic immune responses at single-cell resolution within a microfluidic human skin equivalent
    Article Snippet: .. For single-cell RNA sequencing (scRNA-seq), the samples were removed from the frames and digested with 300 μl of 50 FU/ml nattokinase (MedChem Express) dissolved in sterile DPBS for 1 h at 37 °C 5 % CO 2 . ..

    Article Title: Replicating Dynamic Immune Responses at Single‐Cell Resolution within a Microfluidic Human Skin Equivalent
    Article Snippet: .. For single‐cell RNA sequencing (scRNA‐seq), the samples were removed from the frames and digested with 300 μL of 50 FU mL −1 nattokinase (MedChem Express) dissolved in sterile DPBS for 1 h at 37 °C 5% CO 2 . ..

    RNA Sequencing:

    Article Title: Replicating Dynamic Immune Responses at Single-Cell Resolution within a Microfluidic Human Skin Equivalent.
    Article Snippet: .. For single-cell RNA sequencing (scRNA-seq), the samples were removed from the frames and digested with 300 μL of 50 FU mL−1 nattokinase (MedChem Express) dissolved in sterile DPBS for 1 h at 37 °C 5% CO2. ..

    Article Title: Replicating dynamic immune responses at single-cell resolution within a microfluidic human skin equivalent
    Article Snippet: .. For single-cell RNA sequencing (scRNA-seq), the samples were removed from the frames and digested with 300 μl of 50 FU/ml nattokinase (MedChem Express) dissolved in sterile DPBS for 1 h at 37 °C 5 % CO 2 . ..

    Article Title: Replicating Dynamic Immune Responses at Single‐Cell Resolution within a Microfluidic Human Skin Equivalent
    Article Snippet: .. For single‐cell RNA sequencing (scRNA‐seq), the samples were removed from the frames and digested with 300 μL of 50 FU mL −1 nattokinase (MedChem Express) dissolved in sterile DPBS for 1 h at 37 °C 5% CO 2 . ..

    Sterility:

    Article Title: Replicating Dynamic Immune Responses at Single-Cell Resolution within a Microfluidic Human Skin Equivalent.
    Article Snippet: .. For single-cell RNA sequencing (scRNA-seq), the samples were removed from the frames and digested with 300 μL of 50 FU mL−1 nattokinase (MedChem Express) dissolved in sterile DPBS for 1 h at 37 °C 5% CO2. ..

    Article Title: Replicating dynamic immune responses at single-cell resolution within a microfluidic human skin equivalent
    Article Snippet: .. For single-cell RNA sequencing (scRNA-seq), the samples were removed from the frames and digested with 300 μl of 50 FU/ml nattokinase (MedChem Express) dissolved in sterile DPBS for 1 h at 37 °C 5 % CO 2 . ..

    Article Title: Replicating Dynamic Immune Responses at Single‐Cell Resolution within a Microfluidic Human Skin Equivalent
    Article Snippet: .. For single‐cell RNA sequencing (scRNA‐seq), the samples were removed from the frames and digested with 300 μL of 50 FU mL −1 nattokinase (MedChem Express) dissolved in sterile DPBS for 1 h at 37 °C 5% CO 2 . ..

    Concentration Assay:

    Article Title: Biomechanical stimulation promotes blood vessel growth despite VEGFR-2 inhibition
    Article Snippet: .. Briefly, nattokinase (MedChem Express, HY-P2373) was solubilized 1 mM EDTA in 1 × PBS, resulting in a final concentration of 100 fibrin-degrading units (FU)/mL. ..

    other:

    Article Title: Nuclear factor erythroid 2-related factor 2/heme oxygenase-1 activation by nattokinase reduces pro-inflammatory and matrix-degrading mediators in human gingival fibroblasts
    Article Snippet: Heme Oxygenase-1-IN1 hydrochloride (Cat. #HY-111798A), nattokinase (Cat. #HYP2373), PD98059 (Cat. #HY-12028), NS-398 (Cat. #HY13913), LY294002 (Cat. #HY-10108), SH-5 (Cat. #HY-14766), and SB-242235 (Cat. #HY-16518) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).

    Article Title: Nuclear factor erythroid 2-related factor 2/heme oxygenase-1 activation by nattokinase reduces pro-inflammatory and matrix-degrading mediators in human gingival fibroblasts
    Article Snippet: Heme Oxygenase-1-IN-1 hydrochloride (Cat. #HY-111798A), nattokinase (Cat. #HY-P2373), PD98059 (Cat. #HY-12028), NS-398 (Cat. #HY-13913), LY294002 (Cat. #HY-10108), SH-5 (Cat. #HY-14766), and SB-242235 (Cat. #HY-16518) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).



    Similar Products

    94
    MedChemExpress nattokinase, natto fermentation
    Nattokinase, Natto Fermentation, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hy-p2373/Nattokinase%2C+Natto+fermentation/custom%40hy-p2373%4010%2E64898%2F2026%2E07%2E08%2E735777
    Average 94 stars, based on 1 article reviews
    nattokinase, natto fermentation - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    94
    MedChemExpress nattokinase
    <t>Nattokinase</t> inhibits PM-induced COX-2 and MMP-1 expression. (A) Cells were exposed to different concentrations of nattokinase for various durations, after which cell viability was measured. Cells were pretreated with nattokinase for 1 h, followed by PM exposure, and then (B) COX-2 protein expression, (C) COX-2 mRNA levels, (D) PGE 2 release, and (E) MMP-1 mRNA expression and MMP-1 release were measured. Data are presented as mean ± SD from at least three independent experiments. # P < 0.01 compared with cells exposed to PM alone. PM: particulate matter; COX-2: cyclooxygenase-2; PGE 2 : prostaglandin E 2 ; MMP-1: matrix metalloproteinase-1.
    Nattokinase, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hy-p2373/Nattokinase/pmc12826023-25-5-27
    Average 94 stars, based on 1 article reviews
    nattokinase - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    94
    MedChemExpress frames
    <t>Nattokinase</t> inhibits PM-induced COX-2 and MMP-1 expression. (A) Cells were exposed to different concentrations of nattokinase for various durations, after which cell viability was measured. Cells were pretreated with nattokinase for 1 h, followed by PM exposure, and then (B) COX-2 protein expression, (C) COX-2 mRNA levels, (D) PGE 2 release, and (E) MMP-1 mRNA expression and MMP-1 release were measured. Data are presented as mean ± SD from at least three independent experiments. # P < 0.01 compared with cells exposed to PM alone. PM: particulate matter; COX-2: cyclooxygenase-2; PGE 2 : prostaglandin E 2 ; MMP-1: matrix metalloproteinase-1.
    Frames, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hy-p2373/Nattokinase/pm39836544-291-11-22
    Average 94 stars, based on 1 article reviews
    frames - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    94
    MedChemExpress fermentation medium
    Figure 3. Enzyme production in shake flask culture. (A) Change of enzymatic activity during <t>fermentation.</t> Thrombolytic activity was highest at 96 h, and milk-clotting activity was highest at 60 h (n = 3; error bars represent SD). (B) SDS-PAGE patterns of purified nattokinase (NK) and milk-clotting enzyme (MCE).
    Fermentation Medium, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hy-p2373/Nattokinase/pm34218912-37-44-53
    Average 94 stars, based on 1 article reviews
    fermentation medium - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    Image Search Results


    Nattokinase inhibits PM-induced COX-2 and MMP-1 expression. (A) Cells were exposed to different concentrations of nattokinase for various durations, after which cell viability was measured. Cells were pretreated with nattokinase for 1 h, followed by PM exposure, and then (B) COX-2 protein expression, (C) COX-2 mRNA levels, (D) PGE 2 release, and (E) MMP-1 mRNA expression and MMP-1 release were measured. Data are presented as mean ± SD from at least three independent experiments. # P < 0.01 compared with cells exposed to PM alone. PM: particulate matter; COX-2: cyclooxygenase-2; PGE 2 : prostaglandin E 2 ; MMP-1: matrix metalloproteinase-1.

    Journal: Journal of Dental Sciences

    Article Title: Nuclear factor erythroid 2-related factor 2/heme oxygenase-1 activation by nattokinase reduces pro-inflammatory and matrix-degrading mediators in human gingival fibroblasts

    doi: 10.1016/j.jds.2025.10.022

    Figure Lengend Snippet: Nattokinase inhibits PM-induced COX-2 and MMP-1 expression. (A) Cells were exposed to different concentrations of nattokinase for various durations, after which cell viability was measured. Cells were pretreated with nattokinase for 1 h, followed by PM exposure, and then (B) COX-2 protein expression, (C) COX-2 mRNA levels, (D) PGE 2 release, and (E) MMP-1 mRNA expression and MMP-1 release were measured. Data are presented as mean ± SD from at least three independent experiments. # P < 0.01 compared with cells exposed to PM alone. PM: particulate matter; COX-2: cyclooxygenase-2; PGE 2 : prostaglandin E 2 ; MMP-1: matrix metalloproteinase-1.

    Article Snippet: Heme Oxygenase-1-IN-1 hydrochloride (Cat. #HY-111798A), nattokinase (Cat. #HY-P2373), PD98059 (Cat. #HY-12028), NS-398 (Cat. #HY-13913), LY294002 (Cat. #HY-10108), SH-5 (Cat. #HY-14766), and SB-242235 (Cat. #HY-16518) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).

    Techniques: Expressing

    Nattokinase inhibits NADPH oxidase/ROS-mediated COX-2 expression. Cells were pretreated with NAC, APO, or DPI for 1 h, followed by PM exposure, and then (A) COX-2 protein expression, (B) COX-2 mRNA levels, (C) PGE 2 release, (D) MMP-1 mRNA expression, and (E) MMP-1 release were examined. After cells were treated with PM for different time points, (F) intracellular ROS generation and (G) NADPH oxidase activity were examined. (H) Cells were pretreated with nattokinase or DPI for 1 h, followed by PM exposure, and intracellular ROS generation and NADPH oxidase activity were examined. Data are presented as mean ± SD from at least three independent experiments. # P < 0.01 compared with cells exposed to PM alone (A-E and H). ∗ P < 0.05, # P < 0.01 compared with the control group (F and G). NADPH: nicotinamide adenine dinucleotide phosphate; ROS: reactive oxygen species; COX-2: cyclooxygenase-2; NAC: N-acetyl- l -cysteine; DPI: diphenyleneiodonium chloride; APO: apocynin; PM: particulate matter; PGE 2 : prostaglandin E 2 ; MMP-1: matrix metalloproteinase-1.

    Journal: Journal of Dental Sciences

    Article Title: Nuclear factor erythroid 2-related factor 2/heme oxygenase-1 activation by nattokinase reduces pro-inflammatory and matrix-degrading mediators in human gingival fibroblasts

    doi: 10.1016/j.jds.2025.10.022

    Figure Lengend Snippet: Nattokinase inhibits NADPH oxidase/ROS-mediated COX-2 expression. Cells were pretreated with NAC, APO, or DPI for 1 h, followed by PM exposure, and then (A) COX-2 protein expression, (B) COX-2 mRNA levels, (C) PGE 2 release, (D) MMP-1 mRNA expression, and (E) MMP-1 release were examined. After cells were treated with PM for different time points, (F) intracellular ROS generation and (G) NADPH oxidase activity were examined. (H) Cells were pretreated with nattokinase or DPI for 1 h, followed by PM exposure, and intracellular ROS generation and NADPH oxidase activity were examined. Data are presented as mean ± SD from at least three independent experiments. # P < 0.01 compared with cells exposed to PM alone (A-E and H). ∗ P < 0.05, # P < 0.01 compared with the control group (F and G). NADPH: nicotinamide adenine dinucleotide phosphate; ROS: reactive oxygen species; COX-2: cyclooxygenase-2; NAC: N-acetyl- l -cysteine; DPI: diphenyleneiodonium chloride; APO: apocynin; PM: particulate matter; PGE 2 : prostaglandin E 2 ; MMP-1: matrix metalloproteinase-1.

    Article Snippet: Heme Oxygenase-1-IN-1 hydrochloride (Cat. #HY-111798A), nattokinase (Cat. #HY-P2373), PD98059 (Cat. #HY-12028), NS-398 (Cat. #HY-13913), LY294002 (Cat. #HY-10108), SH-5 (Cat. #HY-14766), and SB-242235 (Cat. #HY-16518) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).

    Techniques: Expressing, Activity Assay, Control

    Bidirectional regulation of PI3K/Akt and ROS in PM-induced COX-2 and MMP-1 expression. Cells were pretreated with LY294002 (LY) or SH-5 for 1 h, followed by PM exposure, and (A) COX-2 protein expression, (B) COX-2 mRNA levels, (C) PGE 2 release, (D) MMP-1 mRNA expression, and (E) MMP-1 release were examined. (F) Cells were treated with PM for different time points, or (G) pretreated with NAC, APO, DPI, or nattokinase for 1 h followed by PM exposure, and Akt phosphorylation was examined. (H) Cells were pretreated with LY294002 or SH-5 for 1 h, followed by PM exposure, and intracellular ROS generation and NADPH oxidase activity were examined. Data are presented as mean ± SD from at least three independent experiments. # P < 0.01 compared with cells exposed to PM alone (A-E, G, and H). # P < 0.01 compared with the control group (F). PI3K: phosphoinositide 3-kinase; Akt: protein kinase B; ROS: reactive oxygen species; PM: particulate matter; COX-2: cyclooxygenase-2; MMP-1: matrix metalloproteinase-1; PGE 2 : prostaglandin E 2 ; NAC: N-acetyl- l -cysteine; APO: apocynin; DPI: diphenyleneiodonium chloride; NADPH: nicotinamide adenine dinucleotide phosphate.

    Journal: Journal of Dental Sciences

    Article Title: Nuclear factor erythroid 2-related factor 2/heme oxygenase-1 activation by nattokinase reduces pro-inflammatory and matrix-degrading mediators in human gingival fibroblasts

    doi: 10.1016/j.jds.2025.10.022

    Figure Lengend Snippet: Bidirectional regulation of PI3K/Akt and ROS in PM-induced COX-2 and MMP-1 expression. Cells were pretreated with LY294002 (LY) or SH-5 for 1 h, followed by PM exposure, and (A) COX-2 protein expression, (B) COX-2 mRNA levels, (C) PGE 2 release, (D) MMP-1 mRNA expression, and (E) MMP-1 release were examined. (F) Cells were treated with PM for different time points, or (G) pretreated with NAC, APO, DPI, or nattokinase for 1 h followed by PM exposure, and Akt phosphorylation was examined. (H) Cells were pretreated with LY294002 or SH-5 for 1 h, followed by PM exposure, and intracellular ROS generation and NADPH oxidase activity were examined. Data are presented as mean ± SD from at least three independent experiments. # P < 0.01 compared with cells exposed to PM alone (A-E, G, and H). # P < 0.01 compared with the control group (F). PI3K: phosphoinositide 3-kinase; Akt: protein kinase B; ROS: reactive oxygen species; PM: particulate matter; COX-2: cyclooxygenase-2; MMP-1: matrix metalloproteinase-1; PGE 2 : prostaglandin E 2 ; NAC: N-acetyl- l -cysteine; APO: apocynin; DPI: diphenyleneiodonium chloride; NADPH: nicotinamide adenine dinucleotide phosphate.

    Article Snippet: Heme Oxygenase-1-IN-1 hydrochloride (Cat. #HY-111798A), nattokinase (Cat. #HY-P2373), PD98059 (Cat. #HY-12028), NS-398 (Cat. #HY-13913), LY294002 (Cat. #HY-10108), SH-5 (Cat. #HY-14766), and SB-242235 (Cat. #HY-16518) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).

    Techniques: Expressing, Phospho-proteomics, Activity Assay, Control

    Nattokinase inhibits p42/p44 MAPK-mediated COX-2 expression. Cells were pretreated with PD98059 for 1 h, followed by PM exposure, and (A) COX-2 protein expression, (B) COX-2 mRNA levels, (C) PGE 2 release, (D) MMP-1 mRNA expression, and (E) MMP-1 release were examined. (F) Cells were treated with PM for different time points, or (G) pretreated with LY294002, NAC, DPI, or nattokinase for 1 h followed by PM exposure, and p42/p44 MAPK phosphorylation was examined. Data are presented as mean ± SD from at least three independent experiments. # P < 0.01 compared with cells exposed to PM alone (A-E and G). # P < 0.01 compared with the control group (F). MAPK: mitogen-activated protein kinase; COX-2: cyclooxygenase-2; PM: particulate matter; PGE 2 : prostaglandin E 2 ; MMP-1: matrix metalloproteinase-1; NAC: N-acetyl- l -cysteine; DPI: diphenyleneiodonium chloride.

    Journal: Journal of Dental Sciences

    Article Title: Nuclear factor erythroid 2-related factor 2/heme oxygenase-1 activation by nattokinase reduces pro-inflammatory and matrix-degrading mediators in human gingival fibroblasts

    doi: 10.1016/j.jds.2025.10.022

    Figure Lengend Snippet: Nattokinase inhibits p42/p44 MAPK-mediated COX-2 expression. Cells were pretreated with PD98059 for 1 h, followed by PM exposure, and (A) COX-2 protein expression, (B) COX-2 mRNA levels, (C) PGE 2 release, (D) MMP-1 mRNA expression, and (E) MMP-1 release were examined. (F) Cells were treated with PM for different time points, or (G) pretreated with LY294002, NAC, DPI, or nattokinase for 1 h followed by PM exposure, and p42/p44 MAPK phosphorylation was examined. Data are presented as mean ± SD from at least three independent experiments. # P < 0.01 compared with cells exposed to PM alone (A-E and G). # P < 0.01 compared with the control group (F). MAPK: mitogen-activated protein kinase; COX-2: cyclooxygenase-2; PM: particulate matter; PGE 2 : prostaglandin E 2 ; MMP-1: matrix metalloproteinase-1; NAC: N-acetyl- l -cysteine; DPI: diphenyleneiodonium chloride.

    Article Snippet: Heme Oxygenase-1-IN-1 hydrochloride (Cat. #HY-111798A), nattokinase (Cat. #HY-P2373), PD98059 (Cat. #HY-12028), NS-398 (Cat. #HY-13913), LY294002 (Cat. #HY-10108), SH-5 (Cat. #HY-14766), and SB-242235 (Cat. #HY-16518) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).

    Techniques: Expressing, Phospho-proteomics, Control

    Nattokinase inhibits p38 MAPK-mediated COX-2 expression. Cells were pretreated with SB242235 for 1 h, followed by PM exposure, and (A) COX-2 protein expression, (B) COX-2 mRNA levels, (C) PGE 2 release, (D) MMP-1 mRNA expression, and (E) MMP-1 release were examined. (F) Cells were treated with PM for different time points, or (G) pretreated with LY294002, NAC, DPI, or nattokinase for 1 h followed by PM exposure, and p38 MAPK phosphorylation was examined. Data are presented as mean ± SD from at least three independent experiments. # P < 0.01 compared with cells exposed to PM alone (A-E and G). ∗ P < 0.05, # P < 0.01 compared with the control group (F). MAPK: mitogen-activated protein kinase; COX-2: cyclooxygenase-2; PM: particulate matter; PGE 2 : prostaglandin E 2 ; MMP-1: matrix metalloproteinase-1; NAC: N-acetyl- l -cysteine; DPI: diphenyleneiodonium chloride.

    Journal: Journal of Dental Sciences

    Article Title: Nuclear factor erythroid 2-related factor 2/heme oxygenase-1 activation by nattokinase reduces pro-inflammatory and matrix-degrading mediators in human gingival fibroblasts

    doi: 10.1016/j.jds.2025.10.022

    Figure Lengend Snippet: Nattokinase inhibits p38 MAPK-mediated COX-2 expression. Cells were pretreated with SB242235 for 1 h, followed by PM exposure, and (A) COX-2 protein expression, (B) COX-2 mRNA levels, (C) PGE 2 release, (D) MMP-1 mRNA expression, and (E) MMP-1 release were examined. (F) Cells were treated with PM for different time points, or (G) pretreated with LY294002, NAC, DPI, or nattokinase for 1 h followed by PM exposure, and p38 MAPK phosphorylation was examined. Data are presented as mean ± SD from at least three independent experiments. # P < 0.01 compared with cells exposed to PM alone (A-E and G). ∗ P < 0.05, # P < 0.01 compared with the control group (F). MAPK: mitogen-activated protein kinase; COX-2: cyclooxygenase-2; PM: particulate matter; PGE 2 : prostaglandin E 2 ; MMP-1: matrix metalloproteinase-1; NAC: N-acetyl- l -cysteine; DPI: diphenyleneiodonium chloride.

    Article Snippet: Heme Oxygenase-1-IN-1 hydrochloride (Cat. #HY-111798A), nattokinase (Cat. #HY-P2373), PD98059 (Cat. #HY-12028), NS-398 (Cat. #HY-13913), LY294002 (Cat. #HY-10108), SH-5 (Cat. #HY-14766), and SB-242235 (Cat. #HY-16518) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).

    Techniques: Expressing, Phospho-proteomics, Control

    Nattokinase suppresses COX-2 and MMP-1 via Nrf2/HO-1 activation. Cells were pretreated with ML385 or Heme Oxygenase-1-IN-1 hydrochloride (HO-1i) for 1 h, with or without nattokinase, followed by PM exposure. (A) COX-2 protein expression, (B) PGE 2 release, and (C) MMP-1 release were examined. Cells were treated for different time points, and (D) HO-1 mRNA levels and (E) ARE-luciferase activity were examined. Data are presented as mean ± SD from at least three independent experiments. # P < 0.01 compared with cells treated with PM plus nattokinase (A–C). ∗ P < 0.05, # P < 0.01 compared with the control group (D and E). COX-2: cyclooxygenase-2; MMP-1: matrix metalloproteinase-1; Nrf2: nuclear factor erythroid 2-related factor 2; HO-1: heme oxygenase-1; PM: particulate matter; PGE 2 : prostaglandin E 2 ; HO-1i: heme oxygenase-1 inhibitor; ARE: antioxidant response element.

    Journal: Journal of Dental Sciences

    Article Title: Nuclear factor erythroid 2-related factor 2/heme oxygenase-1 activation by nattokinase reduces pro-inflammatory and matrix-degrading mediators in human gingival fibroblasts

    doi: 10.1016/j.jds.2025.10.022

    Figure Lengend Snippet: Nattokinase suppresses COX-2 and MMP-1 via Nrf2/HO-1 activation. Cells were pretreated with ML385 or Heme Oxygenase-1-IN-1 hydrochloride (HO-1i) for 1 h, with or without nattokinase, followed by PM exposure. (A) COX-2 protein expression, (B) PGE 2 release, and (C) MMP-1 release were examined. Cells were treated for different time points, and (D) HO-1 mRNA levels and (E) ARE-luciferase activity were examined. Data are presented as mean ± SD from at least three independent experiments. # P < 0.01 compared with cells treated with PM plus nattokinase (A–C). ∗ P < 0.05, # P < 0.01 compared with the control group (D and E). COX-2: cyclooxygenase-2; MMP-1: matrix metalloproteinase-1; Nrf2: nuclear factor erythroid 2-related factor 2; HO-1: heme oxygenase-1; PM: particulate matter; PGE 2 : prostaglandin E 2 ; HO-1i: heme oxygenase-1 inhibitor; ARE: antioxidant response element.

    Article Snippet: Heme Oxygenase-1-IN-1 hydrochloride (Cat. #HY-111798A), nattokinase (Cat. #HY-P2373), PD98059 (Cat. #HY-12028), NS-398 (Cat. #HY-13913), LY294002 (Cat. #HY-10108), SH-5 (Cat. #HY-14766), and SB-242235 (Cat. #HY-16518) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).

    Techniques: Activation Assay, Expressing, Luciferase, Activity Assay, Control

    Schematic illustration of the proposed mechanism. PM stimulation activates multiple signaling pathways, including ROS generation, NADPH oxidase activation, and PI3K/Akt as well as MAPK cascades, leading to the upregulation of COX-2 expression and PGE 2 release, which in turn promote MMP-1 mRNA expression and protein release. Pretreatment with nattokinase attenuates these PM-induced responses through the induction of the Nrf2/HO-1 signaling pathway, thereby suppressing downstream inflammatory and matrix-degrading processes.

    Journal: Journal of Dental Sciences

    Article Title: Nuclear factor erythroid 2-related factor 2/heme oxygenase-1 activation by nattokinase reduces pro-inflammatory and matrix-degrading mediators in human gingival fibroblasts

    doi: 10.1016/j.jds.2025.10.022

    Figure Lengend Snippet: Schematic illustration of the proposed mechanism. PM stimulation activates multiple signaling pathways, including ROS generation, NADPH oxidase activation, and PI3K/Akt as well as MAPK cascades, leading to the upregulation of COX-2 expression and PGE 2 release, which in turn promote MMP-1 mRNA expression and protein release. Pretreatment with nattokinase attenuates these PM-induced responses through the induction of the Nrf2/HO-1 signaling pathway, thereby suppressing downstream inflammatory and matrix-degrading processes.

    Article Snippet: Heme Oxygenase-1-IN-1 hydrochloride (Cat. #HY-111798A), nattokinase (Cat. #HY-P2373), PD98059 (Cat. #HY-12028), NS-398 (Cat. #HY-13913), LY294002 (Cat. #HY-10108), SH-5 (Cat. #HY-14766), and SB-242235 (Cat. #HY-16518) were purchased from MedChemExpress (Monmouth Junction, NJ, USA).

    Techniques: Protein-Protein interactions, Activation Assay, Expressing

    Figure 3. Enzyme production in shake flask culture. (A) Change of enzymatic activity during fermentation. Thrombolytic activity was highest at 96 h, and milk-clotting activity was highest at 60 h (n = 3; error bars represent SD). (B) SDS-PAGE patterns of purified nattokinase (NK) and milk-clotting enzyme (MCE).

    Journal: Journal of dairy science

    Article Title: Screening of a Bacillus subtilis strain producing both nattokinase and milk-clotting enzyme and its application in fermented milk with thrombolytic activity.

    doi: 10.3168/jds.2020-19756

    Figure Lengend Snippet: Figure 3. Enzyme production in shake flask culture. (A) Change of enzymatic activity during fermentation. Thrombolytic activity was highest at 96 h, and milk-clotting activity was highest at 60 h (n = 3; error bars represent SD). (B) SDS-PAGE patterns of purified nattokinase (NK) and milk-clotting enzyme (MCE).

    Article Snippet: Activation medium (for activation of the preserved strain) consisted of 0.5% peptone, 1% glucose, 0.5% NaCl, and water; amplification medium (for shaking flask amplification of the strains) consisted of 1.0% peptone, 0.5% yeast powder, 2% glucose, 0.2% NaH2PO4, 0.4% K2HPO4, 0.5% NaCl, and water; fermentation medium (for shaking flask production of nattokinase and MCE with strains) consisted of 3% kidney bean flour, 2% sucrose, 0.2% NaH2PO4, 0.4% K2HPO4, 0.4% NaCl, 0.02% CaCl2, and water.

    Techniques: Activity Assay, Coagulation, SDS Page, Purification

    Figure 5. The change in bacterial count (cfu), pH, and thrombolytic activity during milk fermentation (n = 3; error bars represent SD).

    Journal: Journal of dairy science

    Article Title: Screening of a Bacillus subtilis strain producing both nattokinase and milk-clotting enzyme and its application in fermented milk with thrombolytic activity.

    doi: 10.3168/jds.2020-19756

    Figure Lengend Snippet: Figure 5. The change in bacterial count (cfu), pH, and thrombolytic activity during milk fermentation (n = 3; error bars represent SD).

    Article Snippet: Activation medium (for activation of the preserved strain) consisted of 0.5% peptone, 1% glucose, 0.5% NaCl, and water; amplification medium (for shaking flask amplification of the strains) consisted of 1.0% peptone, 0.5% yeast powder, 2% glucose, 0.2% NaH2PO4, 0.4% K2HPO4, 0.5% NaCl, and water; fermentation medium (for shaking flask production of nattokinase and MCE with strains) consisted of 3% kidney bean flour, 2% sucrose, 0.2% NaH2PO4, 0.4% K2HPO4, 0.4% NaCl, 0.02% CaCl2, and water.

    Techniques: Activity Assay