hcmec d3 cells  (MedChemExpress)

Journal: Journal of Nanobiotechnology

Article Title: Application of an intercellular adhesion molecule-1-targeted nanoparticles loaded with irisin in postoperative neurocognitive disorder through metabolic axis activation and barrier restoration

doi: 10.1186/s12951-026-04271-y

Figure Lengend Snippet: Establishment and characterization of an inflammatory tricellular BBB in vitro model. ( A ) Changes in TEER of hCMEC/D3 cells before and after LPS treatment, with LPS stimulation significantly reducing TEER levels. ( B ) Permeability assay using FITC-dextran, showing an approximately 2.6-fold increase in permeability following LPS stimulation, indicating barrier function impairment. ( C ) Immunofluorescence reveals MAP2⁺ neuronal processes extending along GFAP⁺ astrocytic end-feet, forming synapse-endfoot contacts. ( D ) Top-down view of cellular co-localization in the tricellular co-culture system, displaying neurons, astrocytes, and endothelial cells co-existing within the same field of view. ( E ) Schematic representation of the spatial distribution within the tricellular in vitro BBB model, illustrating the arrangement of different cells in the Transwell system. ( F ) VE-cadherin immunostaining shows changes in endothelial cell junction integrity before and after LPS treatment, with blurred junctional boundaries and reduced expression post-stimulation. Data are presented as mean ± SEM. Statistical significance in panels A and B was determined using a two-tailed unpaired Student’s t-test, **p < 0.01, ***p < 0.001

Article Snippet: In the inflammatory hCMEC/D3 model, cells were pretreated with the AMPK inhibitor Compound C (10 μM, MCE) for 1 h prior to nanoparticle exposure.

Techniques: In Vitro, Permeability, Immunofluorescence, Co-Culture Assay, Immunostaining, Expressing, Two Tailed Test

Journal: Journal of Nanobiotechnology

Article Title: Application of an intercellular adhesion molecule-1-targeted nanoparticles loaded with irisin in postoperative neurocognitive disorder through metabolic axis activation and barrier restoration

doi: 10.1186/s12951-026-04271-y

Figure Lengend Snippet: Enhanced targeted uptake of ICAM-1-NP@Irisin in inflammatory brain endothelial cells. ( A ) Confocal laser scanning micrographs: Representative fluorescence images of ICAM-1-NP@Irisin and NP@Irisin uptake in inflammatory hCMEC/D3 cells at 1 h, 3 h, and 6 h (red indicates NP fluorescence, blue indicates DAPI nuclear staining, scale bar 25 μm); the ICAM-1-NP@Irisin group exhibits stronger fluorescence signals with higher distribution density. ( B ) Quantitative analysis of fluorescence intensity: At all time points, intracellular fluorescence signals in the ICAM-1-NP@Irisin group are significantly higher than those in the NP@Irisin group (1 h: 1.4 ± 0.1 fold; 3 h: 1.8 ± 0.2 fold; 6 h: 2.1 ± 0.2 fold; data are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA followed by Tukey’s multiple comparisons test, ****p < 0.0001).

Article Snippet: In the inflammatory hCMEC/D3 model, cells were pretreated with the AMPK inhibitor Compound C (10 μM, MCE) for 1 h prior to nanoparticle exposure.

Techniques: Fluorescence, Staining

Journal: Molecular Therapy Oncology

Article Title: A monoclonal antibody W1 blocks mesothelin-mediated tumor progression

doi: 10.1016/j.omton.2025.201094

Figure Lengend Snippet: Functional validation of MSLN domains (A) Schematic representation of MSLN domain truncations. Domain 1 (D1, aa 296–390), domain 2 (D2, aa 391–486), and domain 3 (D3, aa 487–581) were individually fused to the human IgG1 Fc tag for recombinant expression. Binding activity of MSLN-Fc, D1-Fc, D2-Fc, D3-Fc, and Fc control to human ovarian cancer cell lines OVCAR3 ( B) and SKOV3 (C), as well as the CA125-negative pancreatic cancer cell line PANC-1 (D), was assessed by flow cytometry. Comparative analysis of migration (E) and invasion (F) capabilities between OVCAR3 and OVCAR3 MSLN KO cells. Data were normalized to the OVCAR3 group and are presented as mean ± SD from three biologically independent experiments. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001; data were analyzed by ANOVA followed by Tukey’s multiple comparison test.

Article Snippet: Therefore, a medium containing 2% FBS with MSLN-Fc, D1-Fc, D2-Fc, D3-Fc, Fc (100 nM), W1 mIgG1 or A12H cIgG1 (300 nM), MMPi (1,000 nM, MCE, cat#HY-12169), or PBS was added.

Techniques: Functional Assay, Biomarker Discovery, Recombinant, Expressing, Binding Assay, Activity Assay, Control, Flow Cytometry, Migration, Comparison

Journal: Molecular Therapy Oncology

Article Title: A monoclonal antibody W1 blocks mesothelin-mediated tumor progression

doi: 10.1016/j.omton.2025.201094

Figure Lengend Snippet: MSLN and D1 domain promote ovarian cancer cell migration and invasion through the AKT-MMP7 signaling pathway Wound-healing assays showing migration of OVCAR3 MSLN KO (A) and SKOV3 (B) cells after 48 h treatment with MSLN-Fc, D1-Fc, D2-Fc, D3-Fc, or Fc. Data were normalized to the PBS group. Invasion assays of OVCAR3 MSLN KO (C) and SKOV3 (D) cells under the same treatment conditions for 48 h. RT-qPCR analysis of Mmp7 and Mmp9 mRNA expression in response to MSLN-Fc stimulation over time (E) and at increasing concentrations (F). Western blot showing MMP7 protein expression induced by escalating doses of MSLN-Fc, with Fc as control (G). Western blot analysis of MMP7, E-cadherin, and AKT phosphorylation in OVCAR3 MSLN KO (H) and SKOV3 (I) cells treated for 24 h with 100 nM MSLN-Fc, D1-Fc, D2-Fc, D3-Fc, or Fc. All quantitative data are presented as mean ± SD from three biologically independent experiments. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001; data were analyzed by ANOVA followed by Tukey’s multiple comparison test.

Article Snippet: Therefore, a medium containing 2% FBS with MSLN-Fc, D1-Fc, D2-Fc, D3-Fc, Fc (100 nM), W1 mIgG1 or A12H cIgG1 (300 nM), MMPi (1,000 nM, MCE, cat#HY-12169), or PBS was added.

Techniques: Migration, Quantitative RT-PCR, Expressing, Western Blot, Control, Phospho-proteomics, Comparison