notch1 signaling inhibitor dapt (MedChemExpress)
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notch1 signaling inhibitor dapt
Notch1 Signaling Inhibitor Dapt, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hy-p1985a/Notch+1/pmc12105136-85-26-39
Average 94 stars, based on 24 article reviews
Notch1 Signaling Inhibitor Dapt, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hy-p1985a/Notch+1/pmc12105136-85-26-39
Average 94 stars, based on 24 article reviews
notch1 signaling inhibitor dapt - by Bioz Stars,
2026-10
94/100 stars
Images
1) Product Images from "NOTCH1 inhibition enhances immunogenicity and sensitizes triple-negative breast cancer to immune checkpoint inhibitors"
Article Title: NOTCH1 inhibition enhances immunogenicity and sensitizes triple-negative breast cancer to immune checkpoint inhibitors
Journal: Breast Cancer Research : BCR
doi: 10.1186/s13058-025-02045-2
Figure Legend Snippet: NOTCH1 is overexpressed in TNBC and negatively correlates with survival. ( A ) The volcano plot from GEO database of NOTCH1 expression among TNBC tissues and non-TNBC tissues. ( B ) The Kaplan–Meier curves were used to exhibit cumulative survival among NOTCH1-high and NOTCH1-low patients with breast invasive carcinoma. ( C ) The expression of NOTCH1-FL and NICD were detected by western blot in several human BC cell lines. ( D ) The surface expression of NECD was determined by FACS. ( E ) Representative images of NOTCH1 expression in TNBC tumor tissues and adjacent tissues by immunohistochemistry assay. Scale bars = 100 μm. ** p < 0.01 vs MCF-10 A group
Techniques Used: Expressing, Western Blot, Immunohistochemistry
Figure Legend Snippet: NOTCH1 inhibition upregulates MHC-I molecule and antigen presenting-related genes expression. ( A - B ) The expression of NICD and c-Myc were detected after transfecting with shCtrl and shNOTCH1 in MDA-MB-231 and BT549 cells by western blot. ( C - D ) MDA-MB-231 and BT549 cells were treated with increasing concentration DAPT for 48 h respectively, and cell growth inhibition was determined by the MTT assay. The half-maximal inhibitory concentration (IC 50 ) values of MDA-MB-231 and BT549 cells were calculated. Cells were respectively treated with different concentrations of DAPT for 48 h based on their IC 50 , the expression of NICD and c-Myc were detected by western blot. ( E – F ) After NOTCH1 knock-down, the expression of antigen presenting-related genes was measured by qRT-PCR, ( G - H ) and the surface expression of MHC-I molecule was determined by FACS in MDA-MB-231 and BT549 cells. * p < 0.05, ** p < 0.01 vs shCtrl or Control group
Techniques Used: Inhibition, Expressing, Western Blot, Concentration Assay, MTT Assay, Knockdown, Quantitative RT-PCR, Control
Figure Legend Snippet: NOTCH1 inhibition promotes the expression of ICD markers. ( A - B ) After NOTCH1 knockdown or DAPT (48 μM, 72 μM) treatment of MDA-MB-231 and BT549 cells, the surface expression of CALR was determined by FACS. ( C ) Immunofluorescence staining to evaluate CALR translocation in MDA-MB-231 cells. Scale bar = 10 μm. ( D ) Assessment of ATP released into cells culture supernatants by chemiluminescence method. ( E – F ) The ER stress-related proteins expression was detected by western blot. * p < 0.05, ** p < 0.01 vs shCtrl or Control group
Techniques Used: Inhibition, Expressing, Knockdown, Immunofluorescence, Staining, Translocation Assay, Western Blot, Control
Figure Legend Snippet: NOTCH1 inhibition induces cell senescence and SASP generation. ( A - B ) After NOTCH1 knockdown or DAPT (48 μM, 72 μM) treatment of MDA-MB-231 and BT549 cells, EdU staining was performed to label cells, and then flow cytometry assays were used to detect fluorescence for evaluating cell proliferation. ( C ) Cell senescence was detected by SA-β-Gal staining. Scale bar = 50 μm. ( D - E ) Senescence-related proteins expression was detected by western blot. ( F - G ) The mRNA levels were detected by qRT-PCR. ( H - I ) Intracellular production of IFN-λ2 and IFN-β was determined by FACS. ( J ) The protein levels of JAK1, STAT1, and p-STAT1 were tested by western blot in BT549 cells. * p < 0.05, ** p < 0.01 vs shCtrl or Control group
Techniques Used: Inhibition, Knockdown, Staining, Flow Cytometry, Fluorescence, Expressing, Western Blot, Quantitative RT-PCR, Control
Figure Legend Snippet: NOTCH1 inhibition induced senescence and immunogenicity enhancement depends on ATM activation. ( A ) The expression of NOTCH1 and p-ATM in TNBC samples was measured by immunohistochemistry assay. Scale bars = 100 μm. ( B ) After NOTCH1 knock-down in MDA-MB-231 and BT549 cells, the expression levels of p-ATM and ATM were detected by western blot. ( C ) Senescence was detected by SA-β-Gal staining after MDA-MB-231 cells treated with shNOTCH1 plasmid or KU-60019 (3 μM). Scale bar = 50 μm. ( D ) The expression of senescence-related protein in MDA-MB-231 shCtrl and shNOTCH1 cells, or shCtrl and shNOTCH1 cells treated with KU-60019 (3 μM) were detected by western blot. ( E – F ) The protein expression levels of ATM, p-ATM and p-Rb was shown by western blot, and the surface expression of CALR was determined by FACS in shCtrl and shNOTCH1 MDA-MB-231 cells treated with Floxuridine (10 μM) or KU-60019 (3 μM). ( G ) The surface expression of CALR and MHC-I were measured by FACS in shCtrl and shNOTCH1 BT549 cells treated with Floxuridine (10 μM) or KU-60019 (3 μM). ( H ) Immunofluorescence assays demonstrated the intracellular distribution of ATM and NOTCH1 in shCtrl and shNOTCH1 BT549 cells. ( I ) Nonspecific IgG, NICD-Ab or ATM-Ab were used for immunoprecipitation and probed with NICD-Ab or ATM-Ab. Input represents the expression of NICD and ATM in total cell lysates. * p < 0.05, ** p < 0.01 vs shCtrl group
Techniques Used: Inhibition, Immunopeptidomics, Activation Assay, Expressing, Immunohistochemistry, Knockdown, Western Blot, Staining, Plasmid Preparation, Immunofluorescence, Immunoprecipitation
Figure Legend Snippet: Inhibition of NOTCH1 signaling enhances tumor immunogenicity in 4 T1-NICD cells. ( A ) The expression levels of p-ATM and NICD were detected by western blot in Vector and 4T1-NICD cells. ( B ) 4T1-NICD cells were treated with different concentration DAPT for 48 h, and the IC 50 values were calculated. ( C ) FACS analysis showed the surface expression of MHC-I and CALR in Vector, 4T1-NICD and DAPT (120 μM) treated 4T1-NICD cells. ( D ) The ER stress-related proteins expression was detected by western blot. ( E ) The mRNA levels of IFN-β were monitored by qRT-PCR. * p < 0.05, ** p < 0.01 vs Vector group, # p < 0.05 and ## p < 0.01 vs 4T1-NICD group
Techniques Used: Inhibition, Immunopeptidomics, Expressing, Western Blot, Plasmid Preparation, Concentration Assay, Quantitative RT-PCR
Figure Legend Snippet: NOTCH1 inhibition therapy combined with ICIs exert impressive anti-tumor effects and promote immune responds. ( A ) Schematic representation of the in vivo vaccination assay drawn by Figdraw. ( B ) Tumor-free survival curves of BALB/c mice vaccinated with freeze-thawed or DAPT (180 μM) or Floxuridine (30 μM) treated 4T1-NICD cells ( n = 10). ( C - D ) Schematic of DAPT, BMS-1, ATM inhibitor and anti-IFNAR treatment in BALB/c mice models. Representative tumor images of mice, n = 6 for each group. ( E ) Average tumor volume in the mice over time was shown as growth curves. ( F ) Average tumor weight at the end of indicated treatment. ( G ) The change of mouse body weight in the process of treatment. ( H - I ) The ratio of tumor-infiltrating CD3 + CD8 + T cells and CD3 + CD4 + T cells. ( J ) The expression of NOTCH1, p-ATM, Ki67 and CD11b in mouse tumor tissues was detected by immunohistochemistry assays. Scale bars = 50 μm. * p < 0.05, ** p < 0.01 vs Control or DAPT + BMS-1 group
Techniques Used: Inhibition, In Vivo, Expressing, Immunohistochemistry, Control
Figure Legend Snippet: Schematic summary of the effects of NOTCH1 inhibition in regulating tumor immunogenicity and ICIs therapy responds. Targeting NOTCH1 signaling leads to cellular senescence and IFNs release thereby enhancing the immunogenicity of tumor cells encompassing CALR exposure, ATP secretion and ER stress activation. Mechanically, NOTCH1 inhibition attenuates the direct binds with ATM, thus promotes phosphorylation of ATM. Immunogenicity enhancement and interferon release contribute to increase antigen presentation characterized by MHC-I upregulation. These events ultimately lead to improvements of immune microenvironment and increase the responds of the ICIs. This figure was drawn using Biorender.com
Techniques Used: Inhibition, Immunopeptidomics, Activation Assay, Phospho-proteomics
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