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human aβ  (MedChemExpress)


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    Structured Review

    MedChemExpress human aβ
    Effects of REST on the expression of synaptic genes. ( <t>A</t> ) REST was successfully knocked down <t>in</t> <t>SH-SY5Y</t> cells. n = 3. ( B ) REST knockdown increased GRIA1 and GRIN2A expression, while declined GRIN1 expression in SH-SY5Y cells. n = 3. ( C ) 10 μM Aβ increased the mRNA expression of GRIA1 and GRIN2A, and decreased GRIN1 mRNA expression. n = 3. ( D ) REST over-expression significantly increased the mRNA expression of GRIN1, which was decreased by 10 μM Aβ in SH-SY5Y cells. n = 3. Independent experiments were performed three times. Data are expressed as mean ± SEM. * p < 0.05, ** p < 0.01 and *** p < 0.001 vs the corresponding control group.
    Human Aβ, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hy-p1363a/%CE%B2-Amyloid+(1-42)%2C+human/pmc08221361-26-10-13
    Average 94 stars, based on 13 article reviews
    human aβ - by Bioz Stars, 2026-10
    94/100 stars

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    1) Product Images from "Repressor element-1 silencing transcription factor regulates glutamate receptors and immediate early genes to affect synaptic plasticity"

    Article Title: Repressor element-1 silencing transcription factor regulates glutamate receptors and immediate early genes to affect synaptic plasticity

    Journal: Aging (Albany NY)

    doi: 10.18632/aging.203118

    Effects of REST on the expression of synaptic genes. ( A ) REST was successfully knocked down in SH-SY5Y cells. n = 3. ( B ) REST knockdown increased GRIA1 and GRIN2A expression, while declined GRIN1 expression in SH-SY5Y cells. n = 3. ( C ) 10 μM Aβ increased the mRNA expression of GRIA1 and GRIN2A, and decreased GRIN1 mRNA expression. n = 3. ( D ) REST over-expression significantly increased the mRNA expression of GRIN1, which was decreased by 10 μM Aβ in SH-SY5Y cells. n = 3. Independent experiments were performed three times. Data are expressed as mean ± SEM. * p < 0.05, ** p < 0.01 and *** p < 0.001 vs the corresponding control group.
    Figure Legend Snippet: Effects of REST on the expression of synaptic genes. ( A ) REST was successfully knocked down in SH-SY5Y cells. n = 3. ( B ) REST knockdown increased GRIA1 and GRIN2A expression, while declined GRIN1 expression in SH-SY5Y cells. n = 3. ( C ) 10 μM Aβ increased the mRNA expression of GRIA1 and GRIN2A, and decreased GRIN1 mRNA expression. n = 3. ( D ) REST over-expression significantly increased the mRNA expression of GRIN1, which was decreased by 10 μM Aβ in SH-SY5Y cells. n = 3. Independent experiments were performed three times. Data are expressed as mean ± SEM. * p < 0.05, ** p < 0.01 and *** p < 0.001 vs the corresponding control group.

    Techniques Used: Expressing, Knockdown, Over Expression, Control

    Effects of REST on IEGs. ( A ) REST knockdown declined ARC expression, while increased NPAS4 and BDNF expression in SH-SY5Y cells. n = 3. ( B ) 10 μM Aβ decreased ARC mRNA expression. n = 3. ( C ) Over-expression of REST significantly increased ARC mRNA expression, which was decreased by 10 μM Aβ. n = 3. ( D ) The ARC protein expression showed a similar trend to its mRNA expression. n = 3. Independent experiments were performed three times. Data are expressed as mean ± SEM. * p < 0.05, ** p < 0.01 and *** p < 0.001 vs the corresponding control group.
    Figure Legend Snippet: Effects of REST on IEGs. ( A ) REST knockdown declined ARC expression, while increased NPAS4 and BDNF expression in SH-SY5Y cells. n = 3. ( B ) 10 μM Aβ decreased ARC mRNA expression. n = 3. ( C ) Over-expression of REST significantly increased ARC mRNA expression, which was decreased by 10 μM Aβ. n = 3. ( D ) The ARC protein expression showed a similar trend to its mRNA expression. n = 3. Independent experiments were performed three times. Data are expressed as mean ± SEM. * p < 0.05, ** p < 0.01 and *** p < 0.001 vs the corresponding control group.

    Techniques Used: Knockdown, Expressing, Over Expression, Control

    Related Articles

    other:

    Article Title: Paraquat exposure triggers amyloid-β and α-synuclein aggregation in the prefrontal cortex of mice: Suppression of microglial phagocytosis via IL-17A.
    Article Snippet: Paraquat (PQ), an environmental neurotoxin, has been demonstrated to induce pathological protein aggregation and thus neurotoxicity.. Nevertheless, the exact mechanisms remain elusive.. In this investigation, we explored the involvement of interleukin-17A (IL-17 A) in the aggregation of amyloid-β (Aβ) and α-synuclein (α-syn) induced by PQ.

    Incubation:

    Article Title: Repressor element-1 silencing transcription factor regulates glutamate receptors and immediate early genes to affect synaptic plasticity
    Article Snippet: Then, 6 × 10 5 cells were plated in 6-well plates coated by 0.05 mg/ml poly-L-lysine (PLL, BBI Life Science Corporation, China) and incubated with B-27 Serum-Free Supplement (Gibco, USA), GlutaMAX Supplement (Gibco, USA) and Neurobasal Medium (Gibco, USA) at 37°C with a mixed gas of 21% O 2 , 5% CO 2 and N 2 . .. For the Aβ treatment, cells were incubated with 10 μM human Aβ 1-42 (MCE, China) for 48 h. For transfection, SH-SY5Y cells or primary mouse hippocampal neurons were plated in 6-well plates at the density of 3 × 10 5 cells/well. .. REST siRNA (Zorin Bio, Shanghai, China) was transfected into cells with Lipofectamine 3000 kit according to the manufacturer’s instructions (Thermo Fisher, USA).

    Transfection:

    Article Title: Repressor element-1 silencing transcription factor regulates glutamate receptors and immediate early genes to affect synaptic plasticity
    Article Snippet: Then, 6 × 10 5 cells were plated in 6-well plates coated by 0.05 mg/ml poly-L-lysine (PLL, BBI Life Science Corporation, China) and incubated with B-27 Serum-Free Supplement (Gibco, USA), GlutaMAX Supplement (Gibco, USA) and Neurobasal Medium (Gibco, USA) at 37°C with a mixed gas of 21% O 2 , 5% CO 2 and N 2 . .. For the Aβ treatment, cells were incubated with 10 μM human Aβ 1-42 (MCE, China) for 48 h. For transfection, SH-SY5Y cells or primary mouse hippocampal neurons were plated in 6-well plates at the density of 3 × 10 5 cells/well. .. REST siRNA (Zorin Bio, Shanghai, China) was transfected into cells with Lipofectamine 3000 kit according to the manufacturer’s instructions (Thermo Fisher, USA).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Efficient amyloid-β degradation in Alzheimer's disease using SPYTACs.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER CA-5f MCE HY-112698 ATG7-IN-2 MCE HY-146130 DC-LC3in-D5 MCE HY-141882 Bafilomycin A1 MCE HY-100558 Chloroquine MCE HY-17589A Pitstop 2 MCE HY-115604 Methyl-β-cyclodextrin MCE HY-101461 β-amyloid (1-42), Human MCE HY-P1363A LRP-1 cluster II protein, Human (HEK293, His) MCE HY-P702520 LRP-1 cluster II protein, Human (HEK293, Fc) MCE HY-P704765 LRP-1 cluster III protein, Human (HEK293, Fc) MCE HY-P704766 LRP-1 cluster IV protein, Human (HEK293, Fc) MCE HY-P704767 ASGR1/ASGPR1 Protein, Human (HEK293, His) MCE HY-P7610 ASGR2 Protein, Human (HEK293, His) MCE HY-P76736 LRPAP1 protein, Human MCE HY-P76478A LRPAP1 protein, Mouse MCE HY-P76479A Critical commercial assays Thio-S Sigma 1326-12-1 Huma Aβ 1-42 Quantikine ELISA Kit R&D Systems DAB142 Huma Aβ 1-40 Quantikine ELISA Kit R&D Systems DAB140B Iron Stain Kit (Prussian Blue Stain) Abcam ab150674 Mouse CD11b microbeads MiltenyiBiotec 130-093-634 Myelin Removal Beads II Miltenyi Biotec 130-096-433 Hito Golgi-Cox OptimStainTM Kit HiTO Biotec HTKNS1125 HieffTM qPCR SYBR Green Master Mix YEASEN 11201ES08 PierceTM Streptavidin Magnetic Beads Thermo Fisher 88816 PierceTM Protein A/G Magnetic Beads Thermo Fisher 88802 pHrodoTM Antibody Labeling Kit Thermo Fisher P36014 PierceTM Biotinylated Protein Interaction Pull-Down Kit Thermo Fisher 21115 Mouse TNF alpha ELISA Kit Invitrogen BMS607-3 Mouse IL-6 ELISA Kit Invitrogen KMC0061 Dynabeads Protein G Invitrogen 10003D Lipofectamine LTX and PLUS reagent Invitrogen 15338100 Mouse ALT/GPT (Alanine Aminotransferase) ELISA Kit Jiangmei Biotechnology M-02543M2 Mouse AST (Aspartate Aminotransferase) ELISA Kit Jiangmei Biotechnology JM-03113M2 Mouse GGT (Gamma-Glutamyl Transferase) ELISA Kit Jiangmei Biotechnology JM-11634M2 Mouse total protein TP ELISA Kit Saipei Biotechnology SP14882 Deposited data Single-cell RNA sequencing data This paper GSA: CRA018421 Experimental models: Cell lines HEK293T ATCC CRL-1573 HeLa ATCC CRM-CCL-2 HepG2 ATCC HB-8065 Huh-7 Procell CL-0120 AML12 Procell CL-0602 U251 Procell CL-0237 SH-SY5Y Procell CL-0208 (Continued on next page) ll OPEN ACCESS Cell 189, 1–19.e1–e10, April 2, 2026 e2 Please cite this article in press as: Teng et al., Efficient amyloid-β degradation in Alzheimer’s disease using SPYTACs, Cell (2026), https:// doi.org/10.1016/j.cell.2026.01.034 Article .. 5×FAD transgenic mice (B6SJL-Tg (APPSwFlLon, PSEN1*M146L*L286V) 6799Vas/Mmjax, 34840-JAX) and APP/PS1 transgenic mice (B6;C3-Tg(APPswe,PSEN1dE9)85Dbo/Mmjax) were purchased from the Jackson Laboratory.

    Staining:

    Article Title: Efficient amyloid-β degradation in Alzheimer's disease using SPYTACs.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER CA-5f MCE HY-112698 ATG7-IN-2 MCE HY-146130 DC-LC3in-D5 MCE HY-141882 Bafilomycin A1 MCE HY-100558 Chloroquine MCE HY-17589A Pitstop 2 MCE HY-115604 Methyl-β-cyclodextrin MCE HY-101461 β-amyloid (1-42), Human MCE HY-P1363A LRP-1 cluster II protein, Human (HEK293, His) MCE HY-P702520 LRP-1 cluster II protein, Human (HEK293, Fc) MCE HY-P704765 LRP-1 cluster III protein, Human (HEK293, Fc) MCE HY-P704766 LRP-1 cluster IV protein, Human (HEK293, Fc) MCE HY-P704767 ASGR1/ASGPR1 Protein, Human (HEK293, His) MCE HY-P7610 ASGR2 Protein, Human (HEK293, His) MCE HY-P76736 LRPAP1 protein, Human MCE HY-P76478A LRPAP1 protein, Mouse MCE HY-P76479A Critical commercial assays Thio-S Sigma 1326-12-1 Huma Aβ 1-42 Quantikine ELISA Kit R&D Systems DAB142 Huma Aβ 1-40 Quantikine ELISA Kit R&D Systems DAB140B Iron Stain Kit (Prussian Blue Stain) Abcam ab150674 Mouse CD11b microbeads MiltenyiBiotec 130-093-634 Myelin Removal Beads II Miltenyi Biotec 130-096-433 Hito Golgi-Cox OptimStainTM Kit HiTO Biotec HTKNS1125 HieffTM qPCR SYBR Green Master Mix YEASEN 11201ES08 PierceTM Streptavidin Magnetic Beads Thermo Fisher 88816 PierceTM Protein A/G Magnetic Beads Thermo Fisher 88802 pHrodoTM Antibody Labeling Kit Thermo Fisher P36014 PierceTM Biotinylated Protein Interaction Pull-Down Kit Thermo Fisher 21115 Mouse TNF alpha ELISA Kit Invitrogen BMS607-3 Mouse IL-6 ELISA Kit Invitrogen KMC0061 Dynabeads Protein G Invitrogen 10003D Lipofectamine LTX and PLUS reagent Invitrogen 15338100 Mouse ALT/GPT (Alanine Aminotransferase) ELISA Kit Jiangmei Biotechnology M-02543M2 Mouse AST (Aspartate Aminotransferase) ELISA Kit Jiangmei Biotechnology JM-03113M2 Mouse GGT (Gamma-Glutamyl Transferase) ELISA Kit Jiangmei Biotechnology JM-11634M2 Mouse total protein TP ELISA Kit Saipei Biotechnology SP14882 Deposited data Single-cell RNA sequencing data This paper GSA: CRA018421 Experimental models: Cell lines HEK293T ATCC CRL-1573 HeLa ATCC CRM-CCL-2 HepG2 ATCC HB-8065 Huh-7 Procell CL-0120 AML12 Procell CL-0602 U251 Procell CL-0237 SH-SY5Y Procell CL-0208 (Continued on next page) ll OPEN ACCESS Cell 189, 1–19.e1–e10, April 2, 2026 e2 Please cite this article in press as: Teng et al., Efficient amyloid-β degradation in Alzheimer’s disease using SPYTACs, Cell (2026), https:// doi.org/10.1016/j.cell.2026.01.034 Article .. 5×FAD transgenic mice (B6SJL-Tg (APPSwFlLon, PSEN1*M146L*L286V) 6799Vas/Mmjax, 34840-JAX) and APP/PS1 transgenic mice (B6;C3-Tg(APPswe,PSEN1dE9)85Dbo/Mmjax) were purchased from the Jackson Laboratory.

    Real-time Polymerase Chain Reaction:

    Article Title: Efficient amyloid-β degradation in Alzheimer's disease using SPYTACs.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER CA-5f MCE HY-112698 ATG7-IN-2 MCE HY-146130 DC-LC3in-D5 MCE HY-141882 Bafilomycin A1 MCE HY-100558 Chloroquine MCE HY-17589A Pitstop 2 MCE HY-115604 Methyl-β-cyclodextrin MCE HY-101461 β-amyloid (1-42), Human MCE HY-P1363A LRP-1 cluster II protein, Human (HEK293, His) MCE HY-P702520 LRP-1 cluster II protein, Human (HEK293, Fc) MCE HY-P704765 LRP-1 cluster III protein, Human (HEK293, Fc) MCE HY-P704766 LRP-1 cluster IV protein, Human (HEK293, Fc) MCE HY-P704767 ASGR1/ASGPR1 Protein, Human (HEK293, His) MCE HY-P7610 ASGR2 Protein, Human (HEK293, His) MCE HY-P76736 LRPAP1 protein, Human MCE HY-P76478A LRPAP1 protein, Mouse MCE HY-P76479A Critical commercial assays Thio-S Sigma 1326-12-1 Huma Aβ 1-42 Quantikine ELISA Kit R&D Systems DAB142 Huma Aβ 1-40 Quantikine ELISA Kit R&D Systems DAB140B Iron Stain Kit (Prussian Blue Stain) Abcam ab150674 Mouse CD11b microbeads MiltenyiBiotec 130-093-634 Myelin Removal Beads II Miltenyi Biotec 130-096-433 Hito Golgi-Cox OptimStainTM Kit HiTO Biotec HTKNS1125 HieffTM qPCR SYBR Green Master Mix YEASEN 11201ES08 PierceTM Streptavidin Magnetic Beads Thermo Fisher 88816 PierceTM Protein A/G Magnetic Beads Thermo Fisher 88802 pHrodoTM Antibody Labeling Kit Thermo Fisher P36014 PierceTM Biotinylated Protein Interaction Pull-Down Kit Thermo Fisher 21115 Mouse TNF alpha ELISA Kit Invitrogen BMS607-3 Mouse IL-6 ELISA Kit Invitrogen KMC0061 Dynabeads Protein G Invitrogen 10003D Lipofectamine LTX and PLUS reagent Invitrogen 15338100 Mouse ALT/GPT (Alanine Aminotransferase) ELISA Kit Jiangmei Biotechnology M-02543M2 Mouse AST (Aspartate Aminotransferase) ELISA Kit Jiangmei Biotechnology JM-03113M2 Mouse GGT (Gamma-Glutamyl Transferase) ELISA Kit Jiangmei Biotechnology JM-11634M2 Mouse total protein TP ELISA Kit Saipei Biotechnology SP14882 Deposited data Single-cell RNA sequencing data This paper GSA: CRA018421 Experimental models: Cell lines HEK293T ATCC CRL-1573 HeLa ATCC CRM-CCL-2 HepG2 ATCC HB-8065 Huh-7 Procell CL-0120 AML12 Procell CL-0602 U251 Procell CL-0237 SH-SY5Y Procell CL-0208 (Continued on next page) ll OPEN ACCESS Cell 189, 1–19.e1–e10, April 2, 2026 e2 Please cite this article in press as: Teng et al., Efficient amyloid-β degradation in Alzheimer’s disease using SPYTACs, Cell (2026), https:// doi.org/10.1016/j.cell.2026.01.034 Article .. 5×FAD transgenic mice (B6SJL-Tg (APPSwFlLon, PSEN1*M146L*L286V) 6799Vas/Mmjax, 34840-JAX) and APP/PS1 transgenic mice (B6;C3-Tg(APPswe,PSEN1dE9)85Dbo/Mmjax) were purchased from the Jackson Laboratory.

    SYBR Green Assay:

    Article Title: Efficient amyloid-β degradation in Alzheimer's disease using SPYTACs.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER CA-5f MCE HY-112698 ATG7-IN-2 MCE HY-146130 DC-LC3in-D5 MCE HY-141882 Bafilomycin A1 MCE HY-100558 Chloroquine MCE HY-17589A Pitstop 2 MCE HY-115604 Methyl-β-cyclodextrin MCE HY-101461 β-amyloid (1-42), Human MCE HY-P1363A LRP-1 cluster II protein, Human (HEK293, His) MCE HY-P702520 LRP-1 cluster II protein, Human (HEK293, Fc) MCE HY-P704765 LRP-1 cluster III protein, Human (HEK293, Fc) MCE HY-P704766 LRP-1 cluster IV protein, Human (HEK293, Fc) MCE HY-P704767 ASGR1/ASGPR1 Protein, Human (HEK293, His) MCE HY-P7610 ASGR2 Protein, Human (HEK293, His) MCE HY-P76736 LRPAP1 protein, Human MCE HY-P76478A LRPAP1 protein, Mouse MCE HY-P76479A Critical commercial assays Thio-S Sigma 1326-12-1 Huma Aβ 1-42 Quantikine ELISA Kit R&D Systems DAB142 Huma Aβ 1-40 Quantikine ELISA Kit R&D Systems DAB140B Iron Stain Kit (Prussian Blue Stain) Abcam ab150674 Mouse CD11b microbeads MiltenyiBiotec 130-093-634 Myelin Removal Beads II Miltenyi Biotec 130-096-433 Hito Golgi-Cox OptimStainTM Kit HiTO Biotec HTKNS1125 HieffTM qPCR SYBR Green Master Mix YEASEN 11201ES08 PierceTM Streptavidin Magnetic Beads Thermo Fisher 88816 PierceTM Protein A/G Magnetic Beads Thermo Fisher 88802 pHrodoTM Antibody Labeling Kit Thermo Fisher P36014 PierceTM Biotinylated Protein Interaction Pull-Down Kit Thermo Fisher 21115 Mouse TNF alpha ELISA Kit Invitrogen BMS607-3 Mouse IL-6 ELISA Kit Invitrogen KMC0061 Dynabeads Protein G Invitrogen 10003D Lipofectamine LTX and PLUS reagent Invitrogen 15338100 Mouse ALT/GPT (Alanine Aminotransferase) ELISA Kit Jiangmei Biotechnology M-02543M2 Mouse AST (Aspartate Aminotransferase) ELISA Kit Jiangmei Biotechnology JM-03113M2 Mouse GGT (Gamma-Glutamyl Transferase) ELISA Kit Jiangmei Biotechnology JM-11634M2 Mouse total protein TP ELISA Kit Saipei Biotechnology SP14882 Deposited data Single-cell RNA sequencing data This paper GSA: CRA018421 Experimental models: Cell lines HEK293T ATCC CRL-1573 HeLa ATCC CRM-CCL-2 HepG2 ATCC HB-8065 Huh-7 Procell CL-0120 AML12 Procell CL-0602 U251 Procell CL-0237 SH-SY5Y Procell CL-0208 (Continued on next page) ll OPEN ACCESS Cell 189, 1–19.e1–e10, April 2, 2026 e2 Please cite this article in press as: Teng et al., Efficient amyloid-β degradation in Alzheimer’s disease using SPYTACs, Cell (2026), https:// doi.org/10.1016/j.cell.2026.01.034 Article .. 5×FAD transgenic mice (B6SJL-Tg (APPSwFlLon, PSEN1*M146L*L286V) 6799Vas/Mmjax, 34840-JAX) and APP/PS1 transgenic mice (B6;C3-Tg(APPswe,PSEN1dE9)85Dbo/Mmjax) were purchased from the Jackson Laboratory.

    Magnetic Beads:

    Article Title: Efficient amyloid-β degradation in Alzheimer's disease using SPYTACs.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER CA-5f MCE HY-112698 ATG7-IN-2 MCE HY-146130 DC-LC3in-D5 MCE HY-141882 Bafilomycin A1 MCE HY-100558 Chloroquine MCE HY-17589A Pitstop 2 MCE HY-115604 Methyl-β-cyclodextrin MCE HY-101461 β-amyloid (1-42), Human MCE HY-P1363A LRP-1 cluster II protein, Human (HEK293, His) MCE HY-P702520 LRP-1 cluster II protein, Human (HEK293, Fc) MCE HY-P704765 LRP-1 cluster III protein, Human (HEK293, Fc) MCE HY-P704766 LRP-1 cluster IV protein, Human (HEK293, Fc) MCE HY-P704767 ASGR1/ASGPR1 Protein, Human (HEK293, His) MCE HY-P7610 ASGR2 Protein, Human (HEK293, His) MCE HY-P76736 LRPAP1 protein, Human MCE HY-P76478A LRPAP1 protein, Mouse MCE HY-P76479A Critical commercial assays Thio-S Sigma 1326-12-1 Huma Aβ 1-42 Quantikine ELISA Kit R&D Systems DAB142 Huma Aβ 1-40 Quantikine ELISA Kit R&D Systems DAB140B Iron Stain Kit (Prussian Blue Stain) Abcam ab150674 Mouse CD11b microbeads MiltenyiBiotec 130-093-634 Myelin Removal Beads II Miltenyi Biotec 130-096-433 Hito Golgi-Cox OptimStainTM Kit HiTO Biotec HTKNS1125 HieffTM qPCR SYBR Green Master Mix YEASEN 11201ES08 PierceTM Streptavidin Magnetic Beads Thermo Fisher 88816 PierceTM Protein A/G Magnetic Beads Thermo Fisher 88802 pHrodoTM Antibody Labeling Kit Thermo Fisher P36014 PierceTM Biotinylated Protein Interaction Pull-Down Kit Thermo Fisher 21115 Mouse TNF alpha ELISA Kit Invitrogen BMS607-3 Mouse IL-6 ELISA Kit Invitrogen KMC0061 Dynabeads Protein G Invitrogen 10003D Lipofectamine LTX and PLUS reagent Invitrogen 15338100 Mouse ALT/GPT (Alanine Aminotransferase) ELISA Kit Jiangmei Biotechnology M-02543M2 Mouse AST (Aspartate Aminotransferase) ELISA Kit Jiangmei Biotechnology JM-03113M2 Mouse GGT (Gamma-Glutamyl Transferase) ELISA Kit Jiangmei Biotechnology JM-11634M2 Mouse total protein TP ELISA Kit Saipei Biotechnology SP14882 Deposited data Single-cell RNA sequencing data This paper GSA: CRA018421 Experimental models: Cell lines HEK293T ATCC CRL-1573 HeLa ATCC CRM-CCL-2 HepG2 ATCC HB-8065 Huh-7 Procell CL-0120 AML12 Procell CL-0602 U251 Procell CL-0237 SH-SY5Y Procell CL-0208 (Continued on next page) ll OPEN ACCESS Cell 189, 1–19.e1–e10, April 2, 2026 e2 Please cite this article in press as: Teng et al., Efficient amyloid-β degradation in Alzheimer’s disease using SPYTACs, Cell (2026), https:// doi.org/10.1016/j.cell.2026.01.034 Article .. 5×FAD transgenic mice (B6SJL-Tg (APPSwFlLon, PSEN1*M146L*L286V) 6799Vas/Mmjax, 34840-JAX) and APP/PS1 transgenic mice (B6;C3-Tg(APPswe,PSEN1dE9)85Dbo/Mmjax) were purchased from the Jackson Laboratory.

    Antibody Labeling:

    Article Title: Efficient amyloid-β degradation in Alzheimer's disease using SPYTACs.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER CA-5f MCE HY-112698 ATG7-IN-2 MCE HY-146130 DC-LC3in-D5 MCE HY-141882 Bafilomycin A1 MCE HY-100558 Chloroquine MCE HY-17589A Pitstop 2 MCE HY-115604 Methyl-β-cyclodextrin MCE HY-101461 β-amyloid (1-42), Human MCE HY-P1363A LRP-1 cluster II protein, Human (HEK293, His) MCE HY-P702520 LRP-1 cluster II protein, Human (HEK293, Fc) MCE HY-P704765 LRP-1 cluster III protein, Human (HEK293, Fc) MCE HY-P704766 LRP-1 cluster IV protein, Human (HEK293, Fc) MCE HY-P704767 ASGR1/ASGPR1 Protein, Human (HEK293, His) MCE HY-P7610 ASGR2 Protein, Human (HEK293, His) MCE HY-P76736 LRPAP1 protein, Human MCE HY-P76478A LRPAP1 protein, Mouse MCE HY-P76479A Critical commercial assays Thio-S Sigma 1326-12-1 Huma Aβ 1-42 Quantikine ELISA Kit R&D Systems DAB142 Huma Aβ 1-40 Quantikine ELISA Kit R&D Systems DAB140B Iron Stain Kit (Prussian Blue Stain) Abcam ab150674 Mouse CD11b microbeads MiltenyiBiotec 130-093-634 Myelin Removal Beads II Miltenyi Biotec 130-096-433 Hito Golgi-Cox OptimStainTM Kit HiTO Biotec HTKNS1125 HieffTM qPCR SYBR Green Master Mix YEASEN 11201ES08 PierceTM Streptavidin Magnetic Beads Thermo Fisher 88816 PierceTM Protein A/G Magnetic Beads Thermo Fisher 88802 pHrodoTM Antibody Labeling Kit Thermo Fisher P36014 PierceTM Biotinylated Protein Interaction Pull-Down Kit Thermo Fisher 21115 Mouse TNF alpha ELISA Kit Invitrogen BMS607-3 Mouse IL-6 ELISA Kit Invitrogen KMC0061 Dynabeads Protein G Invitrogen 10003D Lipofectamine LTX and PLUS reagent Invitrogen 15338100 Mouse ALT/GPT (Alanine Aminotransferase) ELISA Kit Jiangmei Biotechnology M-02543M2 Mouse AST (Aspartate Aminotransferase) ELISA Kit Jiangmei Biotechnology JM-03113M2 Mouse GGT (Gamma-Glutamyl Transferase) ELISA Kit Jiangmei Biotechnology JM-11634M2 Mouse total protein TP ELISA Kit Saipei Biotechnology SP14882 Deposited data Single-cell RNA sequencing data This paper GSA: CRA018421 Experimental models: Cell lines HEK293T ATCC CRL-1573 HeLa ATCC CRM-CCL-2 HepG2 ATCC HB-8065 Huh-7 Procell CL-0120 AML12 Procell CL-0602 U251 Procell CL-0237 SH-SY5Y Procell CL-0208 (Continued on next page) ll OPEN ACCESS Cell 189, 1–19.e1–e10, April 2, 2026 e2 Please cite this article in press as: Teng et al., Efficient amyloid-β degradation in Alzheimer’s disease using SPYTACs, Cell (2026), https:// doi.org/10.1016/j.cell.2026.01.034 Article .. 5×FAD transgenic mice (B6SJL-Tg (APPSwFlLon, PSEN1*M146L*L286V) 6799Vas/Mmjax, 34840-JAX) and APP/PS1 transgenic mice (B6;C3-Tg(APPswe,PSEN1dE9)85Dbo/Mmjax) were purchased from the Jackson Laboratory.

    AST Assay:

    Article Title: Efficient amyloid-β degradation in Alzheimer's disease using SPYTACs.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER CA-5f MCE HY-112698 ATG7-IN-2 MCE HY-146130 DC-LC3in-D5 MCE HY-141882 Bafilomycin A1 MCE HY-100558 Chloroquine MCE HY-17589A Pitstop 2 MCE HY-115604 Methyl-β-cyclodextrin MCE HY-101461 β-amyloid (1-42), Human MCE HY-P1363A LRP-1 cluster II protein, Human (HEK293, His) MCE HY-P702520 LRP-1 cluster II protein, Human (HEK293, Fc) MCE HY-P704765 LRP-1 cluster III protein, Human (HEK293, Fc) MCE HY-P704766 LRP-1 cluster IV protein, Human (HEK293, Fc) MCE HY-P704767 ASGR1/ASGPR1 Protein, Human (HEK293, His) MCE HY-P7610 ASGR2 Protein, Human (HEK293, His) MCE HY-P76736 LRPAP1 protein, Human MCE HY-P76478A LRPAP1 protein, Mouse MCE HY-P76479A Critical commercial assays Thio-S Sigma 1326-12-1 Huma Aβ 1-42 Quantikine ELISA Kit R&D Systems DAB142 Huma Aβ 1-40 Quantikine ELISA Kit R&D Systems DAB140B Iron Stain Kit (Prussian Blue Stain) Abcam ab150674 Mouse CD11b microbeads MiltenyiBiotec 130-093-634 Myelin Removal Beads II Miltenyi Biotec 130-096-433 Hito Golgi-Cox OptimStainTM Kit HiTO Biotec HTKNS1125 HieffTM qPCR SYBR Green Master Mix YEASEN 11201ES08 PierceTM Streptavidin Magnetic Beads Thermo Fisher 88816 PierceTM Protein A/G Magnetic Beads Thermo Fisher 88802 pHrodoTM Antibody Labeling Kit Thermo Fisher P36014 PierceTM Biotinylated Protein Interaction Pull-Down Kit Thermo Fisher 21115 Mouse TNF alpha ELISA Kit Invitrogen BMS607-3 Mouse IL-6 ELISA Kit Invitrogen KMC0061 Dynabeads Protein G Invitrogen 10003D Lipofectamine LTX and PLUS reagent Invitrogen 15338100 Mouse ALT/GPT (Alanine Aminotransferase) ELISA Kit Jiangmei Biotechnology M-02543M2 Mouse AST (Aspartate Aminotransferase) ELISA Kit Jiangmei Biotechnology JM-03113M2 Mouse GGT (Gamma-Glutamyl Transferase) ELISA Kit Jiangmei Biotechnology JM-11634M2 Mouse total protein TP ELISA Kit Saipei Biotechnology SP14882 Deposited data Single-cell RNA sequencing data This paper GSA: CRA018421 Experimental models: Cell lines HEK293T ATCC CRL-1573 HeLa ATCC CRM-CCL-2 HepG2 ATCC HB-8065 Huh-7 Procell CL-0120 AML12 Procell CL-0602 U251 Procell CL-0237 SH-SY5Y Procell CL-0208 (Continued on next page) ll OPEN ACCESS Cell 189, 1–19.e1–e10, April 2, 2026 e2 Please cite this article in press as: Teng et al., Efficient amyloid-β degradation in Alzheimer’s disease using SPYTACs, Cell (2026), https:// doi.org/10.1016/j.cell.2026.01.034 Article .. 5×FAD transgenic mice (B6SJL-Tg (APPSwFlLon, PSEN1*M146L*L286V) 6799Vas/Mmjax, 34840-JAX) and APP/PS1 transgenic mice (B6;C3-Tg(APPswe,PSEN1dE9)85Dbo/Mmjax) were purchased from the Jackson Laboratory.

    Single Cell:

    Article Title: Efficient amyloid-β degradation in Alzheimer's disease using SPYTACs.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER CA-5f MCE HY-112698 ATG7-IN-2 MCE HY-146130 DC-LC3in-D5 MCE HY-141882 Bafilomycin A1 MCE HY-100558 Chloroquine MCE HY-17589A Pitstop 2 MCE HY-115604 Methyl-β-cyclodextrin MCE HY-101461 β-amyloid (1-42), Human MCE HY-P1363A LRP-1 cluster II protein, Human (HEK293, His) MCE HY-P702520 LRP-1 cluster II protein, Human (HEK293, Fc) MCE HY-P704765 LRP-1 cluster III protein, Human (HEK293, Fc) MCE HY-P704766 LRP-1 cluster IV protein, Human (HEK293, Fc) MCE HY-P704767 ASGR1/ASGPR1 Protein, Human (HEK293, His) MCE HY-P7610 ASGR2 Protein, Human (HEK293, His) MCE HY-P76736 LRPAP1 protein, Human MCE HY-P76478A LRPAP1 protein, Mouse MCE HY-P76479A Critical commercial assays Thio-S Sigma 1326-12-1 Huma Aβ 1-42 Quantikine ELISA Kit R&D Systems DAB142 Huma Aβ 1-40 Quantikine ELISA Kit R&D Systems DAB140B Iron Stain Kit (Prussian Blue Stain) Abcam ab150674 Mouse CD11b microbeads MiltenyiBiotec 130-093-634 Myelin Removal Beads II Miltenyi Biotec 130-096-433 Hito Golgi-Cox OptimStainTM Kit HiTO Biotec HTKNS1125 HieffTM qPCR SYBR Green Master Mix YEASEN 11201ES08 PierceTM Streptavidin Magnetic Beads Thermo Fisher 88816 PierceTM Protein A/G Magnetic Beads Thermo Fisher 88802 pHrodoTM Antibody Labeling Kit Thermo Fisher P36014 PierceTM Biotinylated Protein Interaction Pull-Down Kit Thermo Fisher 21115 Mouse TNF alpha ELISA Kit Invitrogen BMS607-3 Mouse IL-6 ELISA Kit Invitrogen KMC0061 Dynabeads Protein G Invitrogen 10003D Lipofectamine LTX and PLUS reagent Invitrogen 15338100 Mouse ALT/GPT (Alanine Aminotransferase) ELISA Kit Jiangmei Biotechnology M-02543M2 Mouse AST (Aspartate Aminotransferase) ELISA Kit Jiangmei Biotechnology JM-03113M2 Mouse GGT (Gamma-Glutamyl Transferase) ELISA Kit Jiangmei Biotechnology JM-11634M2 Mouse total protein TP ELISA Kit Saipei Biotechnology SP14882 Deposited data Single-cell RNA sequencing data This paper GSA: CRA018421 Experimental models: Cell lines HEK293T ATCC CRL-1573 HeLa ATCC CRM-CCL-2 HepG2 ATCC HB-8065 Huh-7 Procell CL-0120 AML12 Procell CL-0602 U251 Procell CL-0237 SH-SY5Y Procell CL-0208 (Continued on next page) ll OPEN ACCESS Cell 189, 1–19.e1–e10, April 2, 2026 e2 Please cite this article in press as: Teng et al., Efficient amyloid-β degradation in Alzheimer’s disease using SPYTACs, Cell (2026), https:// doi.org/10.1016/j.cell.2026.01.034 Article .. 5×FAD transgenic mice (B6SJL-Tg (APPSwFlLon, PSEN1*M146L*L286V) 6799Vas/Mmjax, 34840-JAX) and APP/PS1 transgenic mice (B6;C3-Tg(APPswe,PSEN1dE9)85Dbo/Mmjax) were purchased from the Jackson Laboratory.

    RNA Sequencing:

    Article Title: Efficient amyloid-β degradation in Alzheimer's disease using SPYTACs.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER CA-5f MCE HY-112698 ATG7-IN-2 MCE HY-146130 DC-LC3in-D5 MCE HY-141882 Bafilomycin A1 MCE HY-100558 Chloroquine MCE HY-17589A Pitstop 2 MCE HY-115604 Methyl-β-cyclodextrin MCE HY-101461 β-amyloid (1-42), Human MCE HY-P1363A LRP-1 cluster II protein, Human (HEK293, His) MCE HY-P702520 LRP-1 cluster II protein, Human (HEK293, Fc) MCE HY-P704765 LRP-1 cluster III protein, Human (HEK293, Fc) MCE HY-P704766 LRP-1 cluster IV protein, Human (HEK293, Fc) MCE HY-P704767 ASGR1/ASGPR1 Protein, Human (HEK293, His) MCE HY-P7610 ASGR2 Protein, Human (HEK293, His) MCE HY-P76736 LRPAP1 protein, Human MCE HY-P76478A LRPAP1 protein, Mouse MCE HY-P76479A Critical commercial assays Thio-S Sigma 1326-12-1 Huma Aβ 1-42 Quantikine ELISA Kit R&D Systems DAB142 Huma Aβ 1-40 Quantikine ELISA Kit R&D Systems DAB140B Iron Stain Kit (Prussian Blue Stain) Abcam ab150674 Mouse CD11b microbeads MiltenyiBiotec 130-093-634 Myelin Removal Beads II Miltenyi Biotec 130-096-433 Hito Golgi-Cox OptimStainTM Kit HiTO Biotec HTKNS1125 HieffTM qPCR SYBR Green Master Mix YEASEN 11201ES08 PierceTM Streptavidin Magnetic Beads Thermo Fisher 88816 PierceTM Protein A/G Magnetic Beads Thermo Fisher 88802 pHrodoTM Antibody Labeling Kit Thermo Fisher P36014 PierceTM Biotinylated Protein Interaction Pull-Down Kit Thermo Fisher 21115 Mouse TNF alpha ELISA Kit Invitrogen BMS607-3 Mouse IL-6 ELISA Kit Invitrogen KMC0061 Dynabeads Protein G Invitrogen 10003D Lipofectamine LTX and PLUS reagent Invitrogen 15338100 Mouse ALT/GPT (Alanine Aminotransferase) ELISA Kit Jiangmei Biotechnology M-02543M2 Mouse AST (Aspartate Aminotransferase) ELISA Kit Jiangmei Biotechnology JM-03113M2 Mouse GGT (Gamma-Glutamyl Transferase) ELISA Kit Jiangmei Biotechnology JM-11634M2 Mouse total protein TP ELISA Kit Saipei Biotechnology SP14882 Deposited data Single-cell RNA sequencing data This paper GSA: CRA018421 Experimental models: Cell lines HEK293T ATCC CRL-1573 HeLa ATCC CRM-CCL-2 HepG2 ATCC HB-8065 Huh-7 Procell CL-0120 AML12 Procell CL-0602 U251 Procell CL-0237 SH-SY5Y Procell CL-0208 (Continued on next page) ll OPEN ACCESS Cell 189, 1–19.e1–e10, April 2, 2026 e2 Please cite this article in press as: Teng et al., Efficient amyloid-β degradation in Alzheimer’s disease using SPYTACs, Cell (2026), https:// doi.org/10.1016/j.cell.2026.01.034 Article .. 5×FAD transgenic mice (B6SJL-Tg (APPSwFlLon, PSEN1*M146L*L286V) 6799Vas/Mmjax, 34840-JAX) and APP/PS1 transgenic mice (B6;C3-Tg(APPswe,PSEN1dE9)85Dbo/Mmjax) were purchased from the Jackson Laboratory.



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    Effects of REST on the expression of synaptic genes. ( A ) REST was successfully knocked down in SH-SY5Y cells. n = 3. ( B ) REST knockdown increased GRIA1 and GRIN2A expression, while declined GRIN1 expression in SH-SY5Y cells. n = 3. ( C ) 10 μM Aβ increased the mRNA expression of GRIA1 and GRIN2A, and decreased GRIN1 mRNA expression. n = 3. ( D ) REST over-expression significantly increased the mRNA expression of GRIN1, which was decreased by 10 μM Aβ in SH-SY5Y cells. n = 3. Independent experiments were performed three times. Data are expressed as mean ± SEM. * p < 0.05, ** p < 0.01 and *** p < 0.001 vs the corresponding control group.

    Journal: Aging (Albany NY)

    Article Title: Repressor element-1 silencing transcription factor regulates glutamate receptors and immediate early genes to affect synaptic plasticity

    doi: 10.18632/aging.203118

    Figure Lengend Snippet: Effects of REST on the expression of synaptic genes. ( A ) REST was successfully knocked down in SH-SY5Y cells. n = 3. ( B ) REST knockdown increased GRIA1 and GRIN2A expression, while declined GRIN1 expression in SH-SY5Y cells. n = 3. ( C ) 10 μM Aβ increased the mRNA expression of GRIA1 and GRIN2A, and decreased GRIN1 mRNA expression. n = 3. ( D ) REST over-expression significantly increased the mRNA expression of GRIN1, which was decreased by 10 μM Aβ in SH-SY5Y cells. n = 3. Independent experiments were performed three times. Data are expressed as mean ± SEM. * p < 0.05, ** p < 0.01 and *** p < 0.001 vs the corresponding control group.

    Article Snippet: For the Aβ treatment, cells were incubated with 10 μM human Aβ 1-42 (MCE, China) for 48 h. For transfection, SH-SY5Y cells or primary mouse hippocampal neurons were plated in 6-well plates at the density of 3 × 10 5 cells/well.

    Techniques: Expressing, Knockdown, Over Expression, Control

    Effects of REST on IEGs. ( A ) REST knockdown declined ARC expression, while increased NPAS4 and BDNF expression in SH-SY5Y cells. n = 3. ( B ) 10 μM Aβ decreased ARC mRNA expression. n = 3. ( C ) Over-expression of REST significantly increased ARC mRNA expression, which was decreased by 10 μM Aβ. n = 3. ( D ) The ARC protein expression showed a similar trend to its mRNA expression. n = 3. Independent experiments were performed three times. Data are expressed as mean ± SEM. * p < 0.05, ** p < 0.01 and *** p < 0.001 vs the corresponding control group.

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    Article Title: Repressor element-1 silencing transcription factor regulates glutamate receptors and immediate early genes to affect synaptic plasticity

    doi: 10.18632/aging.203118

    Figure Lengend Snippet: Effects of REST on IEGs. ( A ) REST knockdown declined ARC expression, while increased NPAS4 and BDNF expression in SH-SY5Y cells. n = 3. ( B ) 10 μM Aβ decreased ARC mRNA expression. n = 3. ( C ) Over-expression of REST significantly increased ARC mRNA expression, which was decreased by 10 μM Aβ. n = 3. ( D ) The ARC protein expression showed a similar trend to its mRNA expression. n = 3. Independent experiments were performed three times. Data are expressed as mean ± SEM. * p < 0.05, ** p < 0.01 and *** p < 0.001 vs the corresponding control group.

    Article Snippet: For the Aβ treatment, cells were incubated with 10 μM human Aβ 1-42 (MCE, China) for 48 h. For transfection, SH-SY5Y cells or primary mouse hippocampal neurons were plated in 6-well plates at the density of 3 × 10 5 cells/well.

    Techniques: Knockdown, Expressing, Over Expression, Control