bam8 22 (MedChemExpress)
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bam8 22
Bam8 22, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hy-p1241/BAM(8-22)/pmc11994654-55-26-29
Average 93 stars, based on 2 article reviews
Bam8 22, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hy-p1241/BAM(8-22)/pmc11994654-55-26-29
Average 93 stars, based on 2 article reviews
bam8 22 - by Bioz Stars,
2026-10
93/100 stars
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1) Product Images from "BAM8-22 targets spinal MrgC receptors to modulate UPR mt activity in the mechanism of bone cancer pain"
Article Title: BAM8-22 targets spinal MrgC receptors to modulate UPR mt activity in the mechanism of bone cancer pain
Journal: Frontiers in Pharmacology
doi: 10.3389/fphar.2025.1575733
Figure Legend Snippet: Effect of BAM8-22 on mitochondrial function. (A) Transmission electron microscopy observations reveal ultrastructural changes in spinal cord mitochondria, with scale bars measuring 2.0 µm (left) and 1.0 µm (right). (B) Mitochondrial ATP levels in the spinal cord are presented. (C, D) Flow histograms and quantitative plots illustrate the mean fluorescence intensity of mitochondrial reactive oxygen species (mtROS) in spinal cord tissue. (E, F) Flow scatter plots and quantitative maps depict mitochondrial membrane potential (MMP) in the spinal cord. (G, H) The PWMT in the right hind limb of mice and the NSF were evaluated 6 hours following the intrathecal administration of BAM8-22 and 21 days post-surgery. An independent t-test was utilized for statistical analysis in comparison to the solvent group (significance levels were indicated as *p < 0.05, **p < 0.01, and ***p < 0.001; n = 6 per group).
Techniques Used: Transmission Assay, Electron Microscopy, Fluorescence, Membrane, Comparison, Solvent
Figure Legend Snippet: Intrathecal injection of BAM8-22 improved mitochondrial function in the dorsal horn of the spinal cord of BCP mice. (A) Representative images depicting MitoSOX in the spinal cord dorsal horn (SCDH) of mice across various groups. Scale bars: 100µm and 50 µm. (B) Mice from the BCP + Vehicle group showed significantly more MitoSOX immunoreactivity in their SCDH compared to the Sham + Vehicle group, according to quantitative analysis. When BAM8-22 was administered to BCP mice, MitoSOX immunoreactivity was decreased. (C) The picture shows that NeuN (shown by the arrow) co-localized with MitoSOX-positive cells. (*p < 0.05, **p < 0.01, ***p < 0.001 vs. respective groups; n = 4 per group).
Techniques Used: Injection
Figure Legend Snippet: BAM8-22 mediates UPR mt activity. (A) The mRNA expression profiles of UPR mt -associated markers (ATF5, HSP60, CLPP, and LONP1) in the ipsilateral spinal cord were quantitatively assessed using RT-PCR, comparing the treatment group with solvent controls. (B) Protein expression levels of ATF5, HSP60, CLPP, and LONP1 in the spinal cord were assessed by Western blotting in both experimental groups. (C) ATF5, HSP60, CLPP, and LONP1 quantitative analysis. (Independent t-test; *p < 0.05, **p < 0.01, ***p < 0.001 in comparison to the solvent group; n = 6 for each group). The mRNA expression profiles of UPR mt -associated markers (ATF5, HSP60, CLPP, and LONP1) in the ipsilateral spinal cord were quantitatively assessed using RT-PCR, comparing the treatment group with solvent controls.
Techniques Used: Activity Assay, Expressing, Reverse Transcription Polymerase Chain Reaction, Solvent, Western Blot, Comparison
Figure Legend Snippet: MrgC regulates cellular UPR mt protein levels. (A, B) The expression levels of ATF5, HSP60, CLPP, and LONP1 as well as the relative expression histograms after BAM8-22 stimulation in N2a cells were evaluated by Western blotting analysis. (C, D) Western blotting was used to assess the expression levels and relative expression histograms of ATF5, HSP60, CLPP, LONP1, and MrgC after Myc-MrgC was overexpressed in N2a cells. (E, F) In 293T cells, Western blotting was similarly conducted after Myc-MrgC overexpression to analyze the expression levels and relative expression histograms of ATF5, HSP60, CLPP, LONP1, and MrgC. (G, H) Western blotting analysis was also performed to examine the protein changes of ATF5, HSP60, CLPP, LONP1, and MrgC, alongside relative expression histograms, following the silencing of MrgC in N2a cells. (Independent t-test, *p < 0.05, **p < 0.01, ***p < 0.001 compared to control; n = 3 per group).
Techniques Used: Expressing, Western Blot, Over Expression, Control
Figure Legend Snippet: By modulating UPR mt activity, BAM8-22 may reduce bone cancer discomfort in mice. ATF5, activating transcription factor 5; HSP60, heat shock protein 60; LONP1, Lon protease 1; CLPP, casein hydrolase; UPR mt , mitochondrial unfolded protein response. Figure Created with BioRender.com .
Techniques Used: Activity Assay
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Control:Article Title: BAM8-22 targets spinal MrgC receptors to modulate UPR mt activity in the mechanism of bone cancer pain Article Snippet: The cells were kept at 37°C in an environment with 5% CO2 (Thermo Forma, USA). .. The experiments investigating cellular drug delivery were classified into two distinct groups: a control group cultured with DMEM, and a BAMB-22 group, which was treated with Cell Culture:Article Title: BAM8-22 targets spinal MrgC receptors to modulate UPR mt activity in the mechanism of bone cancer pain Article Snippet: The cells were kept at 37°C in an environment with 5% CO2 (Thermo Forma, USA). .. The experiments investigating cellular drug delivery were classified into two distinct groups: a control group cultured with DMEM, and a BAMB-22 group, which was treated with |