other:
Article Title: Estrogen-dependent activation of TRX2 reverses oxidative stress and metabolic dysfunction associated with steatotic disease.
Article Snippet: Briefly, AML12 and HepG2 cells are exposed for 24 h to 65 μM sodium oleate (Sigma-Aldrich #O7501) and 45 μM palmitic acid (Sigma-Aldrich #P0500), 100 nM insulin (MedChemExpress #HY-P1156), 4.5 mg/mL glucose (Sigma-Aldrich #G8644) and inflammatory cytokines: 50 ng/mL tumor necrosis factor α (TNFα, Peprotech #HZ-1014), 25 ng/mL interleukin 1β (IL1β, Peprotech #HZ1164) and 8 ng/mL transforming growth factor β (TGFβ, Peprotech #HZ1011). siRNA transfection AML12 and HepG2 cells were seeded into 6-well plates (25 × 104 per well) to achieve 70% confluence the following day, when cells were transfected with either 50 nmol/L siRNA targeting TRX2 (human and mouse siTRX2 SMARTpool, GE Healthcare Dharmacon) or a negative control (nontargeting small interfering RNA, siCTR, GE Healthcare Dharmacon) using Lipofectamine RNAiMAX Reagent (ThermoFisher Scientific #13778-150) and Opti-MEM (GIBCO #31985062), accordingly to manufacturer’s instructions.
Article Title: Exosomes of human adipose stem cells mitigate irradiation injury to salivary glands by inhibiting epithelial-mesenchymal transition through miR-199a-3p targeting Twist1 and regulating TGFβ1/Smad3 pathway
Article Snippet: Cells were cultured in DMEM/F12 medium (C11330500CP, Thermo Fisher Scientific, USA) supplemented with 2.5% FBS, 1% Penicillin-Streptomycin, 200 nM L-glutamine (G0200, Solarbio, China), 5 μg/mL insulin (HY-P1156, MCE, China), 80 ng/mL epidermal growth factor (EGF; PeproTech, USA), 1.1 μM hydrocortisone (G8450, Solarbio, China), 0.03 μg/mL retinoic acid (A9120, Solarbio, China), 2 nM triiodothyronine (HY-A0070, MCE, China), and 5 mg/mL transferrin (T8010, Solarbio, China) in a humidified atmosphere with 5% CO 2 at 37 °C.
Modification:Article Title: Development of Thymic Organoids Heterotopically to Educate and Induce T Lymphocytes
Article Snippet: A total of 100 mesh cell sieve (352360, Corning, USA) was added to PBS after blowing, and centrifuged at 1200 rpm for 5 min. After the supernatant was removed, TECs were added to the complete culture medium for 1.5 h, and centrifuged at 1200 rpm for 5 min. Next, TECs were completely suspended in the culture medium, transferred to a 25 T culture bottle, and cultured in an incubator (Forma Steri‐Cult, ThermoFisher, USA) at 37°C for 48 h with the culture medium refreshed. .. A total of 50 mL TEC complete culture medium consisted of 48.3325 mL dulbecco′s modified eagle medium (DMEM/F12) cell medium (11320033, ThermoFisher, USA), 0.5 mL fetal bovine serum (FBS) (A5670701, Gibco, USA), 0.5 mL P/S solution ( PB180120 , Pricella, China), 0.5 mL l ‐glutamine (ST083, Beyotime, China), 50 μL cholera toxin (HY‐P1446, MCE, USA), 15 μL bovine insulin (HY‐P1156, MCE, USA), 100 μL epidermal growth factor (EGF) (HY‐P7067, MCE, USA), and 2.5 μL hydrocortisone (HY‐N0583, MCE, USA). ..
Magnetic Beads:Article Title: Dual roles of TRPV2 in the innate immune response to cytosolic DNA: Arresting dormant and boosting activated STING.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Insulin from bovine pancreas Sigma-Aldrich Cat#: I1882, CAS: 11070-73-8 Cholera Toxin from Vibrio cholerae Sigma-Aldrich Cat#: C8052, CAS: 9012-63-9 Hydrocortisone Sigma-Aldrich Cat#: H0888, CAS: 50-23-7 Anti-FLAG® M2 Magnetic Beads Sigma-Aldrich Cat#: M8823 CaptivA HF Resins RepliGen Cat#: CA-HF-0025 Tranilast MedChemExpress Cat#: HY-B0195, CAS: 53902-12-8 BAPTA-AM Calbiochem Cat#: 196419, CAS: 126150-97-8 Protease/phosphatase inhibitor Cell Signaling Technology Cat#: 5872 2’,3 ′ -cGAMP Cell Signaling Technology Cat#: 35573, CAS: 1441190-66-4 Animal-Free Recombinant Human EGF PeproTech Cat#: AF-100-15, CAS: 62253-63-8 Hoechst 33342 ThermoFisher Cat#: H3570, CAS: 875756-97-1 2-Mercaptoethanol ThermoFisher Cat#: 21985023, CAS: 60-24-2 Lipofectamine TM RNAiMAX Transfection Reagent ThermoFisher Scientific Cat#: 13778100 Lipofectamine 3000 Transfection Reagent ThermoFisher Scientific Cat#: L3000015 TransIT®-LT1 Transfection Reagent Mirus Cat#: MIR2300 Human IFN-β Immunoassay R&D Systems Cat#: DIFNB Human IL-6 Immunoassay R&D Systems Cat#: D6050 Human IL-1β/IL-1F2 Immunoassay R&D Systems Cat#: DLB50 Human CCL2/MCP-1 Immunoassay R&D Systems Cat#: DCP00 Cell lines HeLa ATCC Cat#: CCL-2 HaCaT ATCC Cat#: PCS-200-011 HEK293T ATCC Cat#: CRL-11268 MCF10A ATCC Cat#: CRL-10317 NK92MI cells ATCC Cat#: CRL-2408 THP-1 ATCC Cat#: TIB-202 Primary Mammary Epithelial Cells (HMEC) ATCC Cat#: PCS-600-010 MOLM-13 Leibniz Institute DSMZ Cat#: ACC 554 Oligonucleotides sgRNA sequence for AMPKα1 This paper GAAGATCGGCCACTACATTC sgRNA sequence for AMPKα2 This paper GAAGATCGGACACTACGTGC sgRNA sequence for CaMKK2 #1 This paper GAGACAGCTTGCGACCGGAG sgRNA sequence for CaMKK2 #2 This paper ACGTGGTGAGACTCCACTGT Stealth RNAi Luciferase Reporter Control (Control) Thermo Fisher siRNA ID: 12935146 siRNA for TRPV2 #1 Thermo Fisher GAACCTGCTTTACTATACA siRNA for TRPV2 #2 Thermo Fisher CCCTAGTGATGATCTCGGA siRNA for cGAS #1 Thermo Fisher GGAAGAAATTAACGACATT siRNA for cGAS #2 Thermo Fisher CCAACACTCGTGCATATTA siRNA for TRAF6 #1 Thermo Fisher GAAGGTTGTTTGCACAAGA siRNA for TRAF6 #2 Thermo Fisher GCCTAATCATTATGATCTA siRNA for TREX1 #1 Thermo Fisher GCATCTACACTCGCCTGTA siRNA for TREX1 #2 Thermo Fisher ACCCTCATCTTTTTCGACA siRNA for FANCD2 #1 Thermo Fisher GCACCGTATTCAAGTACAA siRNA for FANCD2 #2 Thermo Fisher CAGCCTACCTGAGATCCTA siRNA for STIM1 #1 Thermo Fisher GCCTATATCCAGAACCGTT siRNA for STIM1 #2 Thermo Fisher GCAGCAGAGTTTTGCCGAA siRNA for STING #1 Thermo Fisher CCTCATCAGTGGAATGGAA (Continued on next page) 18 Cell Reports 45, 116745, January 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER siRNA for STING #2 Thermo Fisher GGTCATATTACATCGGATA siRNA for TBK1 #1 Thermo Fisher CAACAAGACATAAAGTACT siRNA for TBK1 #2 Thermo Fisher GCCTCGTAGGAATAAAGAA siRNA for AMPKa1 Thermo Fisher GAACCCTGTGACAAGCACA siRNA for AMPKa2 Thermo Fisher GATTTCGGATTATCTAATA shRNA for Luciferase This paper GAATCGTCGTATGCAGTGAAA shRNA for TRPV2 #1 This paper AGCCGGATCCAAACCGATTTG shRNA for TRPV2 #2 This paper CCTAGTGATGATCTCGGACAA shRNA for TRPV2 #UTR This paper GAACCTGCTTGCTGGAGCTTA shRNA for STING #1 This paper GTCCAGG ACTTGACATCTTAA shRNA for STING #2 This paper GCCCGGATTCGAACTTACAAT qPCR primers for TRPV2 This paper Forward: GGAGGTGAACTGGGCTTCATG Reverse: GCACCATCCTCATCCTCCTTG qPCR primers for ACTB This paper Forward: CACCATTGGCAATGAGCGGTTC Reverse: AGGTCTTTGCGGATGTCCACGT qPCR primers for human GAPDH This paper Forward: AACAGCGACACCCACTCCTC Reverse: GGAGGGGAGATTCAGTGTGGT qPCR primers for IFNB This paper Forward: CTTGGATTCCTACAAAGAAGCAGC Reverse: TCCTCCTTCTGGAACTGCTGCA qPCR primers for IL-6 This paper Forward: AGTGAGGAACAAGCCAGAGC Reverse: CATTTGTGGTTGGGTCAGG qPCR primers for IL-1β This paper Forward: CGATCACTGAACTGCACGCTC Reverse: ACAAAGGACATGGAGAACACCACTT qPCR primers for CCL2 This paper Forward: CAGCCAGATGCAATCAATGCC Reverse: TGGAATCCTGAACCCACTTCT Plasmids psPAX2 Addgene Cat#: 12260; RRID: Addgene_12260 pMD2.G Addgene Cat#: 12259; RRID: Addgene_12259 pCDH-CMV-MCS-EF1-Puro (pCDH) NovoPro Cat#: V006738; RRID: 64874 pCDH-Flag This paper N/A pBOB-GCaMP6s This paper N/A pCDH-TRPV2-mCherry (wild-type, truncation or point mutants) This paper N/A pCDH-HA-TRPV2 (wild-type, truncations or mutants) This paper N/A pCDH- STING-Flag (wild-type or mutants) This paper N/A pCDH- STING-HA (wild-type or mutants) This paper N/A pCDH-STING-mNeonGreen This paper N/A pCW-STING-HA (wild-type or mutants) This paper N/A pLKO.1 This paper N/A Software and algorithms GraphPad Prism GraphPad https://www.graphpad.com/ Fiji-ImageJ ImageJ https://imagej.nih.gov/ij/ Image Studio TM Lite LI-COR Biosciences https://www.licor.com/bio/products/ software/image_studio_lite/ BioTek Gen5 Cytation5 https://www.agilent.com Creative Cloud All Apps Adobe https://www.adobe.com/ UCSF Chimera Chimera 1.15 https://www.cgl.ucsf.edu/chimera/ SMART SMART http://smart.embl-heidelberg.de/ (Continued on next page) Cell Reports 45, 116745, January 27, 2026 19
Recombinant:Article Title: Dual roles of TRPV2 in the innate immune response to cytosolic DNA: Arresting dormant and boosting activated STING.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Insulin from bovine pancreas Sigma-Aldrich Cat#: I1882, CAS: 11070-73-8 Cholera Toxin from Vibrio cholerae Sigma-Aldrich Cat#: C8052, CAS: 9012-63-9 Hydrocortisone Sigma-Aldrich Cat#: H0888, CAS: 50-23-7 Anti-FLAG® M2 Magnetic Beads Sigma-Aldrich Cat#: M8823 CaptivA HF Resins RepliGen Cat#: CA-HF-0025 Tranilast MedChemExpress Cat#: HY-B0195, CAS: 53902-12-8 BAPTA-AM Calbiochem Cat#: 196419, CAS: 126150-97-8 Protease/phosphatase inhibitor Cell Signaling Technology Cat#: 5872 2’,3 ′ -cGAMP Cell Signaling Technology Cat#: 35573, CAS: 1441190-66-4 Animal-Free Recombinant Human EGF PeproTech Cat#: AF-100-15, CAS: 62253-63-8 Hoechst 33342 ThermoFisher Cat#: H3570, CAS: 875756-97-1 2-Mercaptoethanol ThermoFisher Cat#: 21985023, CAS: 60-24-2 Lipofectamine TM RNAiMAX Transfection Reagent ThermoFisher Scientific Cat#: 13778100 Lipofectamine 3000 Transfection Reagent ThermoFisher Scientific Cat#: L3000015 TransIT®-LT1 Transfection Reagent Mirus Cat#: MIR2300 Human IFN-β Immunoassay R&D Systems Cat#: DIFNB Human IL-6 Immunoassay R&D Systems Cat#: D6050 Human IL-1β/IL-1F2 Immunoassay R&D Systems Cat#: DLB50 Human CCL2/MCP-1 Immunoassay R&D Systems Cat#: DCP00 Cell lines HeLa ATCC Cat#: CCL-2 HaCaT ATCC Cat#: PCS-200-011 HEK293T ATCC Cat#: CRL-11268 MCF10A ATCC Cat#: CRL-10317 NK92MI cells ATCC Cat#: CRL-2408 THP-1 ATCC Cat#: TIB-202 Primary Mammary Epithelial Cells (HMEC) ATCC Cat#: PCS-600-010 MOLM-13 Leibniz Institute DSMZ Cat#: ACC 554 Oligonucleotides sgRNA sequence for AMPKα1 This paper GAAGATCGGCCACTACATTC sgRNA sequence for AMPKα2 This paper GAAGATCGGACACTACGTGC sgRNA sequence for CaMKK2 #1 This paper GAGACAGCTTGCGACCGGAG sgRNA sequence for CaMKK2 #2 This paper ACGTGGTGAGACTCCACTGT Stealth RNAi Luciferase Reporter Control (Control) Thermo Fisher siRNA ID: 12935146 siRNA for TRPV2 #1 Thermo Fisher GAACCTGCTTTACTATACA siRNA for TRPV2 #2 Thermo Fisher CCCTAGTGATGATCTCGGA siRNA for cGAS #1 Thermo Fisher GGAAGAAATTAACGACATT siRNA for cGAS #2 Thermo Fisher CCAACACTCGTGCATATTA siRNA for TRAF6 #1 Thermo Fisher GAAGGTTGTTTGCACAAGA siRNA for TRAF6 #2 Thermo Fisher GCCTAATCATTATGATCTA siRNA for TREX1 #1 Thermo Fisher GCATCTACACTCGCCTGTA siRNA for TREX1 #2 Thermo Fisher ACCCTCATCTTTTTCGACA siRNA for FANCD2 #1 Thermo Fisher GCACCGTATTCAAGTACAA siRNA for FANCD2 #2 Thermo Fisher CAGCCTACCTGAGATCCTA siRNA for STIM1 #1 Thermo Fisher GCCTATATCCAGAACCGTT siRNA for STIM1 #2 Thermo Fisher GCAGCAGAGTTTTGCCGAA siRNA for STING #1 Thermo Fisher CCTCATCAGTGGAATGGAA (Continued on next page) 18 Cell Reports 45, 116745, January 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER siRNA for STING #2 Thermo Fisher GGTCATATTACATCGGATA siRNA for TBK1 #1 Thermo Fisher CAACAAGACATAAAGTACT siRNA for TBK1 #2 Thermo Fisher GCCTCGTAGGAATAAAGAA siRNA for AMPKa1 Thermo Fisher GAACCCTGTGACAAGCACA siRNA for AMPKa2 Thermo Fisher GATTTCGGATTATCTAATA shRNA for Luciferase This paper GAATCGTCGTATGCAGTGAAA shRNA for TRPV2 #1 This paper AGCCGGATCCAAACCGATTTG shRNA for TRPV2 #2 This paper CCTAGTGATGATCTCGGACAA shRNA for TRPV2 #UTR This paper GAACCTGCTTGCTGGAGCTTA shRNA for STING #1 This paper GTCCAGG ACTTGACATCTTAA shRNA for STING #2 This paper GCCCGGATTCGAACTTACAAT qPCR primers for TRPV2 This paper Forward: GGAGGTGAACTGGGCTTCATG Reverse: GCACCATCCTCATCCTCCTTG qPCR primers for ACTB This paper Forward: CACCATTGGCAATGAGCGGTTC Reverse: AGGTCTTTGCGGATGTCCACGT qPCR primers for human GAPDH This paper Forward: AACAGCGACACCCACTCCTC Reverse: GGAGGGGAGATTCAGTGTGGT qPCR primers for IFNB This paper Forward: CTTGGATTCCTACAAAGAAGCAGC Reverse: TCCTCCTTCTGGAACTGCTGCA qPCR primers for IL-6 This paper Forward: AGTGAGGAACAAGCCAGAGC Reverse: CATTTGTGGTTGGGTCAGG qPCR primers for IL-1β This paper Forward: CGATCACTGAACTGCACGCTC Reverse: ACAAAGGACATGGAGAACACCACTT qPCR primers for CCL2 This paper Forward: CAGCCAGATGCAATCAATGCC Reverse: TGGAATCCTGAACCCACTTCT Plasmids psPAX2 Addgene Cat#: 12260; RRID: Addgene_12260 pMD2.G Addgene Cat#: 12259; RRID: Addgene_12259 pCDH-CMV-MCS-EF1-Puro (pCDH) NovoPro Cat#: V006738; RRID: 64874 pCDH-Flag This paper N/A pBOB-GCaMP6s This paper N/A pCDH-TRPV2-mCherry (wild-type, truncation or point mutants) This paper N/A pCDH-HA-TRPV2 (wild-type, truncations or mutants) This paper N/A pCDH- STING-Flag (wild-type or mutants) This paper N/A pCDH- STING-HA (wild-type or mutants) This paper N/A pCDH-STING-mNeonGreen This paper N/A pCW-STING-HA (wild-type or mutants) This paper N/A pLKO.1 This paper N/A Software and algorithms GraphPad Prism GraphPad https://www.graphpad.com/ Fiji-ImageJ ImageJ https://imagej.nih.gov/ij/ Image Studio TM Lite LI-COR Biosciences https://www.licor.com/bio/products/ software/image_studio_lite/ BioTek Gen5 Cytation5 https://www.agilent.com Creative Cloud All Apps Adobe https://www.adobe.com/ UCSF Chimera Chimera 1.15 https://www.cgl.ucsf.edu/chimera/ SMART SMART http://smart.embl-heidelberg.de/ (Continued on next page) Cell Reports 45, 116745, January 27, 2026 19
Transfection:Article Title: Dual roles of TRPV2 in the innate immune response to cytosolic DNA: Arresting dormant and boosting activated STING.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Insulin from bovine pancreas Sigma-Aldrich Cat#: I1882, CAS: 11070-73-8 Cholera Toxin from Vibrio cholerae Sigma-Aldrich Cat#: C8052, CAS: 9012-63-9 Hydrocortisone Sigma-Aldrich Cat#: H0888, CAS: 50-23-7 Anti-FLAG® M2 Magnetic Beads Sigma-Aldrich Cat#: M8823 CaptivA HF Resins RepliGen Cat#: CA-HF-0025 Tranilast MedChemExpress Cat#: HY-B0195, CAS: 53902-12-8 BAPTA-AM Calbiochem Cat#: 196419, CAS: 126150-97-8 Protease/phosphatase inhibitor Cell Signaling Technology Cat#: 5872 2’,3 ′ -cGAMP Cell Signaling Technology Cat#: 35573, CAS: 1441190-66-4 Animal-Free Recombinant Human EGF PeproTech Cat#: AF-100-15, CAS: 62253-63-8 Hoechst 33342 ThermoFisher Cat#: H3570, CAS: 875756-97-1 2-Mercaptoethanol ThermoFisher Cat#: 21985023, CAS: 60-24-2 Lipofectamine TM RNAiMAX Transfection Reagent ThermoFisher Scientific Cat#: 13778100 Lipofectamine 3000 Transfection Reagent ThermoFisher Scientific Cat#: L3000015 TransIT®-LT1 Transfection Reagent Mirus Cat#: MIR2300 Human IFN-β Immunoassay R&D Systems Cat#: DIFNB Human IL-6 Immunoassay R&D Systems Cat#: D6050 Human IL-1β/IL-1F2 Immunoassay R&D Systems Cat#: DLB50 Human CCL2/MCP-1 Immunoassay R&D Systems Cat#: DCP00 Cell lines HeLa ATCC Cat#: CCL-2 HaCaT ATCC Cat#: PCS-200-011 HEK293T ATCC Cat#: CRL-11268 MCF10A ATCC Cat#: CRL-10317 NK92MI cells ATCC Cat#: CRL-2408 THP-1 ATCC Cat#: TIB-202 Primary Mammary Epithelial Cells (HMEC) ATCC Cat#: PCS-600-010 MOLM-13 Leibniz Institute DSMZ Cat#: ACC 554 Oligonucleotides sgRNA sequence for AMPKα1 This paper GAAGATCGGCCACTACATTC sgRNA sequence for AMPKα2 This paper GAAGATCGGACACTACGTGC sgRNA sequence for CaMKK2 #1 This paper GAGACAGCTTGCGACCGGAG sgRNA sequence for CaMKK2 #2 This paper ACGTGGTGAGACTCCACTGT Stealth RNAi Luciferase Reporter Control (Control) Thermo Fisher siRNA ID: 12935146 siRNA for TRPV2 #1 Thermo Fisher GAACCTGCTTTACTATACA siRNA for TRPV2 #2 Thermo Fisher CCCTAGTGATGATCTCGGA siRNA for cGAS #1 Thermo Fisher GGAAGAAATTAACGACATT siRNA for cGAS #2 Thermo Fisher CCAACACTCGTGCATATTA siRNA for TRAF6 #1 Thermo Fisher GAAGGTTGTTTGCACAAGA siRNA for TRAF6 #2 Thermo Fisher GCCTAATCATTATGATCTA siRNA for TREX1 #1 Thermo Fisher GCATCTACACTCGCCTGTA siRNA for TREX1 #2 Thermo Fisher ACCCTCATCTTTTTCGACA siRNA for FANCD2 #1 Thermo Fisher GCACCGTATTCAAGTACAA siRNA for FANCD2 #2 Thermo Fisher CAGCCTACCTGAGATCCTA siRNA for STIM1 #1 Thermo Fisher GCCTATATCCAGAACCGTT siRNA for STIM1 #2 Thermo Fisher GCAGCAGAGTTTTGCCGAA siRNA for STING #1 Thermo Fisher CCTCATCAGTGGAATGGAA (Continued on next page) 18 Cell Reports 45, 116745, January 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER siRNA for STING #2 Thermo Fisher GGTCATATTACATCGGATA siRNA for TBK1 #1 Thermo Fisher CAACAAGACATAAAGTACT siRNA for TBK1 #2 Thermo Fisher GCCTCGTAGGAATAAAGAA siRNA for AMPKa1 Thermo Fisher GAACCCTGTGACAAGCACA siRNA for AMPKa2 Thermo Fisher GATTTCGGATTATCTAATA shRNA for Luciferase This paper GAATCGTCGTATGCAGTGAAA shRNA for TRPV2 #1 This paper AGCCGGATCCAAACCGATTTG shRNA for TRPV2 #2 This paper CCTAGTGATGATCTCGGACAA shRNA for TRPV2 #UTR This paper GAACCTGCTTGCTGGAGCTTA shRNA for STING #1 This paper GTCCAGG ACTTGACATCTTAA shRNA for STING #2 This paper GCCCGGATTCGAACTTACAAT qPCR primers for TRPV2 This paper Forward: GGAGGTGAACTGGGCTTCATG Reverse: GCACCATCCTCATCCTCCTTG qPCR primers for ACTB This paper Forward: CACCATTGGCAATGAGCGGTTC Reverse: AGGTCTTTGCGGATGTCCACGT qPCR primers for human GAPDH This paper Forward: AACAGCGACACCCACTCCTC Reverse: GGAGGGGAGATTCAGTGTGGT qPCR primers for IFNB This paper Forward: CTTGGATTCCTACAAAGAAGCAGC Reverse: TCCTCCTTCTGGAACTGCTGCA qPCR primers for IL-6 This paper Forward: AGTGAGGAACAAGCCAGAGC Reverse: CATTTGTGGTTGGGTCAGG qPCR primers for IL-1β This paper Forward: CGATCACTGAACTGCACGCTC Reverse: ACAAAGGACATGGAGAACACCACTT qPCR primers for CCL2 This paper Forward: CAGCCAGATGCAATCAATGCC Reverse: TGGAATCCTGAACCCACTTCT Plasmids psPAX2 Addgene Cat#: 12260; RRID: Addgene_12260 pMD2.G Addgene Cat#: 12259; RRID: Addgene_12259 pCDH-CMV-MCS-EF1-Puro (pCDH) NovoPro Cat#: V006738; RRID: 64874 pCDH-Flag This paper N/A pBOB-GCaMP6s This paper N/A pCDH-TRPV2-mCherry (wild-type, truncation or point mutants) This paper N/A pCDH-HA-TRPV2 (wild-type, truncations or mutants) This paper N/A pCDH- STING-Flag (wild-type or mutants) This paper N/A pCDH- STING-HA (wild-type or mutants) This paper N/A pCDH-STING-mNeonGreen This paper N/A pCW-STING-HA (wild-type or mutants) This paper N/A pLKO.1 This paper N/A Software and algorithms GraphPad Prism GraphPad https://www.graphpad.com/ Fiji-ImageJ ImageJ https://imagej.nih.gov/ij/ Image Studio TM Lite LI-COR Biosciences https://www.licor.com/bio/products/ software/image_studio_lite/ BioTek Gen5 Cytation5 https://www.agilent.com Creative Cloud All Apps Adobe https://www.adobe.com/ UCSF Chimera Chimera 1.15 https://www.cgl.ucsf.edu/chimera/ SMART SMART http://smart.embl-heidelberg.de/ (Continued on next page) Cell Reports 45, 116745, January 27, 2026 19
Sequencing:Article Title: Dual roles of TRPV2 in the innate immune response to cytosolic DNA: Arresting dormant and boosting activated STING.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Insulin from bovine pancreas Sigma-Aldrich Cat#: I1882, CAS: 11070-73-8 Cholera Toxin from Vibrio cholerae Sigma-Aldrich Cat#: C8052, CAS: 9012-63-9 Hydrocortisone Sigma-Aldrich Cat#: H0888, CAS: 50-23-7 Anti-FLAG® M2 Magnetic Beads Sigma-Aldrich Cat#: M8823 CaptivA HF Resins RepliGen Cat#: CA-HF-0025 Tranilast MedChemExpress Cat#: HY-B0195, CAS: 53902-12-8 BAPTA-AM Calbiochem Cat#: 196419, CAS: 126150-97-8 Protease/phosphatase inhibitor Cell Signaling Technology Cat#: 5872 2’,3 ′ -cGAMP Cell Signaling Technology Cat#: 35573, CAS: 1441190-66-4 Animal-Free Recombinant Human EGF PeproTech Cat#: AF-100-15, CAS: 62253-63-8 Hoechst 33342 ThermoFisher Cat#: H3570, CAS: 875756-97-1 2-Mercaptoethanol ThermoFisher Cat#: 21985023, CAS: 60-24-2 Lipofectamine TM RNAiMAX Transfection Reagent ThermoFisher Scientific Cat#: 13778100 Lipofectamine 3000 Transfection Reagent ThermoFisher Scientific Cat#: L3000015 TransIT®-LT1 Transfection Reagent Mirus Cat#: MIR2300 Human IFN-β Immunoassay R&D Systems Cat#: DIFNB Human IL-6 Immunoassay R&D Systems Cat#: D6050 Human IL-1β/IL-1F2 Immunoassay R&D Systems Cat#: DLB50 Human CCL2/MCP-1 Immunoassay R&D Systems Cat#: DCP00 Cell lines HeLa ATCC Cat#: CCL-2 HaCaT ATCC Cat#: PCS-200-011 HEK293T ATCC Cat#: CRL-11268 MCF10A ATCC Cat#: CRL-10317 NK92MI cells ATCC Cat#: CRL-2408 THP-1 ATCC Cat#: TIB-202 Primary Mammary Epithelial Cells (HMEC) ATCC Cat#: PCS-600-010 MOLM-13 Leibniz Institute DSMZ Cat#: ACC 554 Oligonucleotides sgRNA sequence for AMPKα1 This paper GAAGATCGGCCACTACATTC sgRNA sequence for AMPKα2 This paper GAAGATCGGACACTACGTGC sgRNA sequence for CaMKK2 #1 This paper GAGACAGCTTGCGACCGGAG sgRNA sequence for CaMKK2 #2 This paper ACGTGGTGAGACTCCACTGT Stealth RNAi Luciferase Reporter Control (Control) Thermo Fisher siRNA ID: 12935146 siRNA for TRPV2 #1 Thermo Fisher GAACCTGCTTTACTATACA siRNA for TRPV2 #2 Thermo Fisher CCCTAGTGATGATCTCGGA siRNA for cGAS #1 Thermo Fisher GGAAGAAATTAACGACATT siRNA for cGAS #2 Thermo Fisher CCAACACTCGTGCATATTA siRNA for TRAF6 #1 Thermo Fisher GAAGGTTGTTTGCACAAGA siRNA for TRAF6 #2 Thermo Fisher GCCTAATCATTATGATCTA siRNA for TREX1 #1 Thermo Fisher GCATCTACACTCGCCTGTA siRNA for TREX1 #2 Thermo Fisher ACCCTCATCTTTTTCGACA siRNA for FANCD2 #1 Thermo Fisher GCACCGTATTCAAGTACAA siRNA for FANCD2 #2 Thermo Fisher CAGCCTACCTGAGATCCTA siRNA for STIM1 #1 Thermo Fisher GCCTATATCCAGAACCGTT siRNA for STIM1 #2 Thermo Fisher GCAGCAGAGTTTTGCCGAA siRNA for STING #1 Thermo Fisher CCTCATCAGTGGAATGGAA (Continued on next page) 18 Cell Reports 45, 116745, January 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER siRNA for STING #2 Thermo Fisher GGTCATATTACATCGGATA siRNA for TBK1 #1 Thermo Fisher CAACAAGACATAAAGTACT siRNA for TBK1 #2 Thermo Fisher GCCTCGTAGGAATAAAGAA siRNA for AMPKa1 Thermo Fisher GAACCCTGTGACAAGCACA siRNA for AMPKa2 Thermo Fisher GATTTCGGATTATCTAATA shRNA for Luciferase This paper GAATCGTCGTATGCAGTGAAA shRNA for TRPV2 #1 This paper AGCCGGATCCAAACCGATTTG shRNA for TRPV2 #2 This paper CCTAGTGATGATCTCGGACAA shRNA for TRPV2 #UTR This paper GAACCTGCTTGCTGGAGCTTA shRNA for STING #1 This paper GTCCAGG ACTTGACATCTTAA shRNA for STING #2 This paper GCCCGGATTCGAACTTACAAT qPCR primers for TRPV2 This paper Forward: GGAGGTGAACTGGGCTTCATG Reverse: GCACCATCCTCATCCTCCTTG qPCR primers for ACTB This paper Forward: CACCATTGGCAATGAGCGGTTC Reverse: AGGTCTTTGCGGATGTCCACGT qPCR primers for human GAPDH This paper Forward: AACAGCGACACCCACTCCTC Reverse: GGAGGGGAGATTCAGTGTGGT qPCR primers for IFNB This paper Forward: CTTGGATTCCTACAAAGAAGCAGC Reverse: TCCTCCTTCTGGAACTGCTGCA qPCR primers for IL-6 This paper Forward: AGTGAGGAACAAGCCAGAGC Reverse: CATTTGTGGTTGGGTCAGG qPCR primers for IL-1β This paper Forward: CGATCACTGAACTGCACGCTC Reverse: ACAAAGGACATGGAGAACACCACTT qPCR primers for CCL2 This paper Forward: CAGCCAGATGCAATCAATGCC Reverse: TGGAATCCTGAACCCACTTCT Plasmids psPAX2 Addgene Cat#: 12260; RRID: Addgene_12260 pMD2.G Addgene Cat#: 12259; RRID: Addgene_12259 pCDH-CMV-MCS-EF1-Puro (pCDH) NovoPro Cat#: V006738; RRID: 64874 pCDH-Flag This paper N/A pBOB-GCaMP6s This paper N/A pCDH-TRPV2-mCherry (wild-type, truncation or point mutants) This paper N/A pCDH-HA-TRPV2 (wild-type, truncations or mutants) This paper N/A pCDH- STING-Flag (wild-type or mutants) This paper N/A pCDH- STING-HA (wild-type or mutants) This paper N/A pCDH-STING-mNeonGreen This paper N/A pCW-STING-HA (wild-type or mutants) This paper N/A pLKO.1 This paper N/A Software and algorithms GraphPad Prism GraphPad https://www.graphpad.com/ Fiji-ImageJ ImageJ https://imagej.nih.gov/ij/ Image Studio TM Lite LI-COR Biosciences https://www.licor.com/bio/products/ software/image_studio_lite/ BioTek Gen5 Cytation5 https://www.agilent.com Creative Cloud All Apps Adobe https://www.adobe.com/ UCSF Chimera Chimera 1.15 https://www.cgl.ucsf.edu/chimera/ SMART SMART http://smart.embl-heidelberg.de/ (Continued on next page) Cell Reports 45, 116745, January 27, 2026 19
Luciferase:Article Title: Dual roles of TRPV2 in the innate immune response to cytosolic DNA: Arresting dormant and boosting activated STING.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Insulin from bovine pancreas Sigma-Aldrich Cat#: I1882, CAS: 11070-73-8 Cholera Toxin from Vibrio cholerae Sigma-Aldrich Cat#: C8052, CAS: 9012-63-9 Hydrocortisone Sigma-Aldrich Cat#: H0888, CAS: 50-23-7 Anti-FLAG® M2 Magnetic Beads Sigma-Aldrich Cat#: M8823 CaptivA HF Resins RepliGen Cat#: CA-HF-0025 Tranilast MedChemExpress Cat#: HY-B0195, CAS: 53902-12-8 BAPTA-AM Calbiochem Cat#: 196419, CAS: 126150-97-8 Protease/phosphatase inhibitor Cell Signaling Technology Cat#: 5872 2’,3 ′ -cGAMP Cell Signaling Technology Cat#: 35573, CAS: 1441190-66-4 Animal-Free Recombinant Human EGF PeproTech Cat#: AF-100-15, CAS: 62253-63-8 Hoechst 33342 ThermoFisher Cat#: H3570, CAS: 875756-97-1 2-Mercaptoethanol ThermoFisher Cat#: 21985023, CAS: 60-24-2 Lipofectamine TM RNAiMAX Transfection Reagent ThermoFisher Scientific Cat#: 13778100 Lipofectamine 3000 Transfection Reagent ThermoFisher Scientific Cat#: L3000015 TransIT®-LT1 Transfection Reagent Mirus Cat#: MIR2300 Human IFN-β Immunoassay R&D Systems Cat#: DIFNB Human IL-6 Immunoassay R&D Systems Cat#: D6050 Human IL-1β/IL-1F2 Immunoassay R&D Systems Cat#: DLB50 Human CCL2/MCP-1 Immunoassay R&D Systems Cat#: DCP00 Cell lines HeLa ATCC Cat#: CCL-2 HaCaT ATCC Cat#: PCS-200-011 HEK293T ATCC Cat#: CRL-11268 MCF10A ATCC Cat#: CRL-10317 NK92MI cells ATCC Cat#: CRL-2408 THP-1 ATCC Cat#: TIB-202 Primary Mammary Epithelial Cells (HMEC) ATCC Cat#: PCS-600-010 MOLM-13 Leibniz Institute DSMZ Cat#: ACC 554 Oligonucleotides sgRNA sequence for AMPKα1 This paper GAAGATCGGCCACTACATTC sgRNA sequence for AMPKα2 This paper GAAGATCGGACACTACGTGC sgRNA sequence for CaMKK2 #1 This paper GAGACAGCTTGCGACCGGAG sgRNA sequence for CaMKK2 #2 This paper ACGTGGTGAGACTCCACTGT Stealth RNAi Luciferase Reporter Control (Control) Thermo Fisher siRNA ID: 12935146 siRNA for TRPV2 #1 Thermo Fisher GAACCTGCTTTACTATACA siRNA for TRPV2 #2 Thermo Fisher CCCTAGTGATGATCTCGGA siRNA for cGAS #1 Thermo Fisher GGAAGAAATTAACGACATT siRNA for cGAS #2 Thermo Fisher CCAACACTCGTGCATATTA siRNA for TRAF6 #1 Thermo Fisher GAAGGTTGTTTGCACAAGA siRNA for TRAF6 #2 Thermo Fisher GCCTAATCATTATGATCTA siRNA for TREX1 #1 Thermo Fisher GCATCTACACTCGCCTGTA siRNA for TREX1 #2 Thermo Fisher ACCCTCATCTTTTTCGACA siRNA for FANCD2 #1 Thermo Fisher GCACCGTATTCAAGTACAA siRNA for FANCD2 #2 Thermo Fisher CAGCCTACCTGAGATCCTA siRNA for STIM1 #1 Thermo Fisher GCCTATATCCAGAACCGTT siRNA for STIM1 #2 Thermo Fisher GCAGCAGAGTTTTGCCGAA siRNA for STING #1 Thermo Fisher CCTCATCAGTGGAATGGAA (Continued on next page) 18 Cell Reports 45, 116745, January 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER siRNA for STING #2 Thermo Fisher GGTCATATTACATCGGATA siRNA for TBK1 #1 Thermo Fisher CAACAAGACATAAAGTACT siRNA for TBK1 #2 Thermo Fisher GCCTCGTAGGAATAAAGAA siRNA for AMPKa1 Thermo Fisher GAACCCTGTGACAAGCACA siRNA for AMPKa2 Thermo Fisher GATTTCGGATTATCTAATA shRNA for Luciferase This paper GAATCGTCGTATGCAGTGAAA shRNA for TRPV2 #1 This paper AGCCGGATCCAAACCGATTTG shRNA for TRPV2 #2 This paper CCTAGTGATGATCTCGGACAA shRNA for TRPV2 #UTR This paper GAACCTGCTTGCTGGAGCTTA shRNA for STING #1 This paper GTCCAGG ACTTGACATCTTAA shRNA for STING #2 This paper GCCCGGATTCGAACTTACAAT qPCR primers for TRPV2 This paper Forward: GGAGGTGAACTGGGCTTCATG Reverse: GCACCATCCTCATCCTCCTTG qPCR primers for ACTB This paper Forward: CACCATTGGCAATGAGCGGTTC Reverse: AGGTCTTTGCGGATGTCCACGT qPCR primers for human GAPDH This paper Forward: AACAGCGACACCCACTCCTC Reverse: GGAGGGGAGATTCAGTGTGGT qPCR primers for IFNB This paper Forward: CTTGGATTCCTACAAAGAAGCAGC Reverse: TCCTCCTTCTGGAACTGCTGCA qPCR primers for IL-6 This paper Forward: AGTGAGGAACAAGCCAGAGC Reverse: CATTTGTGGTTGGGTCAGG qPCR primers for IL-1β This paper Forward: CGATCACTGAACTGCACGCTC Reverse: ACAAAGGACATGGAGAACACCACTT qPCR primers for CCL2 This paper Forward: CAGCCAGATGCAATCAATGCC Reverse: TGGAATCCTGAACCCACTTCT Plasmids psPAX2 Addgene Cat#: 12260; RRID: Addgene_12260 pMD2.G Addgene Cat#: 12259; RRID: Addgene_12259 pCDH-CMV-MCS-EF1-Puro (pCDH) NovoPro Cat#: V006738; RRID: 64874 pCDH-Flag This paper N/A pBOB-GCaMP6s This paper N/A pCDH-TRPV2-mCherry (wild-type, truncation or point mutants) This paper N/A pCDH-HA-TRPV2 (wild-type, truncations or mutants) This paper N/A pCDH- STING-Flag (wild-type or mutants) This paper N/A pCDH- STING-HA (wild-type or mutants) This paper N/A pCDH-STING-mNeonGreen This paper N/A pCW-STING-HA (wild-type or mutants) This paper N/A pLKO.1 This paper N/A Software and algorithms GraphPad Prism GraphPad https://www.graphpad.com/ Fiji-ImageJ ImageJ https://imagej.nih.gov/ij/ Image Studio TM Lite LI-COR Biosciences https://www.licor.com/bio/products/ software/image_studio_lite/ BioTek Gen5 Cytation5 https://www.agilent.com Creative Cloud All Apps Adobe https://www.adobe.com/ UCSF Chimera Chimera 1.15 https://www.cgl.ucsf.edu/chimera/ SMART SMART http://smart.embl-heidelberg.de/ (Continued on next page) Cell Reports 45, 116745, January 27, 2026 19
Control:Article Title: Dual roles of TRPV2 in the innate immune response to cytosolic DNA: Arresting dormant and boosting activated STING.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Insulin from bovine pancreas Sigma-Aldrich Cat#: I1882, CAS: 11070-73-8 Cholera Toxin from Vibrio cholerae Sigma-Aldrich Cat#: C8052, CAS: 9012-63-9 Hydrocortisone Sigma-Aldrich Cat#: H0888, CAS: 50-23-7 Anti-FLAG® M2 Magnetic Beads Sigma-Aldrich Cat#: M8823 CaptivA HF Resins RepliGen Cat#: CA-HF-0025 Tranilast MedChemExpress Cat#: HY-B0195, CAS: 53902-12-8 BAPTA-AM Calbiochem Cat#: 196419, CAS: 126150-97-8 Protease/phosphatase inhibitor Cell Signaling Technology Cat#: 5872 2’,3 ′ -cGAMP Cell Signaling Technology Cat#: 35573, CAS: 1441190-66-4 Animal-Free Recombinant Human EGF PeproTech Cat#: AF-100-15, CAS: 62253-63-8 Hoechst 33342 ThermoFisher Cat#: H3570, CAS: 875756-97-1 2-Mercaptoethanol ThermoFisher Cat#: 21985023, CAS: 60-24-2 Lipofectamine TM RNAiMAX Transfection Reagent ThermoFisher Scientific Cat#: 13778100 Lipofectamine 3000 Transfection Reagent ThermoFisher Scientific Cat#: L3000015 TransIT®-LT1 Transfection Reagent Mirus Cat#: MIR2300 Human IFN-β Immunoassay R&D Systems Cat#: DIFNB Human IL-6 Immunoassay R&D Systems Cat#: D6050 Human IL-1β/IL-1F2 Immunoassay R&D Systems Cat#: DLB50 Human CCL2/MCP-1 Immunoassay R&D Systems Cat#: DCP00 Cell lines HeLa ATCC Cat#: CCL-2 HaCaT ATCC Cat#: PCS-200-011 HEK293T ATCC Cat#: CRL-11268 MCF10A ATCC Cat#: CRL-10317 NK92MI cells ATCC Cat#: CRL-2408 THP-1 ATCC Cat#: TIB-202 Primary Mammary Epithelial Cells (HMEC) ATCC Cat#: PCS-600-010 MOLM-13 Leibniz Institute DSMZ Cat#: ACC 554 Oligonucleotides sgRNA sequence for AMPKα1 This paper GAAGATCGGCCACTACATTC sgRNA sequence for AMPKα2 This paper GAAGATCGGACACTACGTGC sgRNA sequence for CaMKK2 #1 This paper GAGACAGCTTGCGACCGGAG sgRNA sequence for CaMKK2 #2 This paper ACGTGGTGAGACTCCACTGT Stealth RNAi Luciferase Reporter Control (Control) Thermo Fisher siRNA ID: 12935146 siRNA for TRPV2 #1 Thermo Fisher GAACCTGCTTTACTATACA siRNA for TRPV2 #2 Thermo Fisher CCCTAGTGATGATCTCGGA siRNA for cGAS #1 Thermo Fisher GGAAGAAATTAACGACATT siRNA for cGAS #2 Thermo Fisher CCAACACTCGTGCATATTA siRNA for TRAF6 #1 Thermo Fisher GAAGGTTGTTTGCACAAGA siRNA for TRAF6 #2 Thermo Fisher GCCTAATCATTATGATCTA siRNA for TREX1 #1 Thermo Fisher GCATCTACACTCGCCTGTA siRNA for TREX1 #2 Thermo Fisher ACCCTCATCTTTTTCGACA siRNA for FANCD2 #1 Thermo Fisher GCACCGTATTCAAGTACAA siRNA for FANCD2 #2 Thermo Fisher CAGCCTACCTGAGATCCTA siRNA for STIM1 #1 Thermo Fisher GCCTATATCCAGAACCGTT siRNA for STIM1 #2 Thermo Fisher GCAGCAGAGTTTTGCCGAA siRNA for STING #1 Thermo Fisher CCTCATCAGTGGAATGGAA (Continued on next page) 18 Cell Reports 45, 116745, January 27, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER siRNA for STING #2 Thermo Fisher GGTCATATTACATCGGATA siRNA for TBK1 #1 Thermo Fisher CAACAAGACATAAAGTACT siRNA for TBK1 #2 Thermo Fisher GCCTCGTAGGAATAAAGAA siRNA for AMPKa1 Thermo Fisher GAACCCTGTGACAAGCACA siRNA for AMPKa2 Thermo Fisher GATTTCGGATTATCTAATA shRNA for Luciferase This paper GAATCGTCGTATGCAGTGAAA shRNA for TRPV2 #1 This paper AGCCGGATCCAAACCGATTTG shRNA for TRPV2 #2 This paper CCTAGTGATGATCTCGGACAA shRNA for TRPV2 #UTR This paper GAACCTGCTTGCTGGAGCTTA shRNA for STING #1 This paper GTCCAGG ACTTGACATCTTAA shRNA for STING #2 This paper GCCCGGATTCGAACTTACAAT qPCR primers for TRPV2 This paper Forward: GGAGGTGAACTGGGCTTCATG Reverse: GCACCATCCTCATCCTCCTTG qPCR primers for ACTB This paper Forward: CACCATTGGCAATGAGCGGTTC Reverse: AGGTCTTTGCGGATGTCCACGT qPCR primers for human GAPDH This paper Forward: AACAGCGACACCCACTCCTC Reverse: GGAGGGGAGATTCAGTGTGGT qPCR primers for IFNB This paper Forward: CTTGGATTCCTACAAAGAAGCAGC Reverse: TCCTCCTTCTGGAACTGCTGCA qPCR primers for IL-6 This paper Forward: AGTGAGGAACAAGCCAGAGC Reverse: CATTTGTGGTTGGGTCAGG qPCR primers for IL-1β This paper Forward: CGATCACTGAACTGCACGCTC Reverse: ACAAAGGACATGGAGAACACCACTT qPCR primers for CCL2 This paper Forward: CAGCCAGATGCAATCAATGCC Reverse: TGGAATCCTGAACCCACTTCT Plasmids psPAX2 Addgene Cat#: 12260; RRID: Addgene_12260 pMD2.G Addgene Cat#: 12259; RRID: Addgene_12259 pCDH-CMV-MCS-EF1-Puro (pCDH) NovoPro Cat#: V006738; RRID: 64874 pCDH-Flag This paper N/A pBOB-GCaMP6s This paper N/A pCDH-TRPV2-mCherry (wild-type, truncation or point mutants) This paper N/A pCDH-HA-TRPV2 (wild-type, truncations or mutants) This paper N/A pCDH- STING-Flag (wild-type or mutants) This paper N/A pCDH- STING-HA (wild-type or mutants) This paper N/A pCDH-STING-mNeonGreen This paper N/A pCW-STING-HA (wild-type or mutants) This paper N/A pLKO.1 This paper N/A Software and algorithms GraphPad Prism GraphPad https://www.graphpad.com/ Fiji-ImageJ ImageJ https://imagej.nih.gov/ij/ Image Studio TM Lite LI-COR Biosciences https://www.licor.com/bio/products/ software/image_studio_lite/ BioTek Gen5 Cytation5 https://www.agilent.com Creative Cloud All Apps Adobe https://www.adobe.com/ UCSF Chimera Chimera 1.15 https://www.cgl.ucsf.edu/chimera/ SMART SMART http://smart.embl-heidelberg.de/ (Continued on next page) Cell Reports 45, 116745, January 27, 2026 19
|
MedChemExpress is a verified supplier 
MedChemExpress manufactures this product 