tc14012 (MedChemExpress)

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MedChemExpress tc14012
Tc14012, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hy-p1102/TC14012/pm37141639-124-0-7
Average 93 stars, based on 6 article reviews

tc14012 - by Bioz Stars, 2026-10

93/100 stars

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Synthesized:

Article Title: Atypical activation and molecular glue-like dimerization mechanism of an intrinsically-biased chemokine receptor
Article Snippet: .. VUF11207 was synthesized as described previously while VUF10661 was purchased from Sigma-Aldrich (Cat. No: SML0803) and TC14012 was purchased from MedChemExpress (Cat. no: HY-P1102). .. The opioid peptides used in βarr recruitment assays and structural studies were obtained from Genscript.

Activation Assay:

Article Title: CXCR7 agonist TC14012 improves angiogenic function of endothelial progenitor cells via activating Akt/eNOS pathway and promotes ischemic angiogenesis in diabetic limb ischemia
Article Snippet: For signal test, EPCs were harvested at 10 min after mannitol or HG with or without TC14012 (5 μmol/L, Cayman Chemical Company, Ann Arbor, MI) treatment. .. To confirm the critical role of Akt pathway in mediating the protective effect of TC14012, 200 nmol/L wortmannin (MedChemExpress, Shanghai, China) was used to inhibit the activation of Akt signal. .. To knockdown CXCR7 expression in EPCs, siRNAs against human CXCR7 or a Silencer Select Negative Control (GenePharma, Shanghai, China) were transfected into EPCs using GP-transfect-Mate (GenePharma, Shanghai, China).

Article Title: CXCR7 agonist TC14012 improves angiogenic function of endothelial progenitor cells via activating Akt/eNOS pathway and promotes ischemic angiogenesis in diabetic limb ischemia
Article Snippet: For signal test, EPCs were harvested at 10 min after mannitol or HG with or without TC14012 (5 μmol/L, Cayman Chemical Company, Ann Arbor, MI) treatment. .. To confirm the critical role of Akt pathway in mediating the protective effect of TC14012, 200 nmol/L wortmannin (MedChemExpress, Shanghai, China) was used to inhibit the activation of Akt signal. siRNA transfection To knockdown CXCR7 expression in EPCs, siRNAs against human CXCR7 or a Silencer Select Negative Control (GenePharma, Shanghai, China) were transfected into EPCs using GP-transfect-Mate (GenePharma, Shanghai, China). ..

other:

Article Title: Regulation of the Hippo/YAP axis by CXCR7 in the tumorigenesis of gastric cancer.
Article Snippet: The reagents used were XMU-MP-1 (MCE, Cat. No. HY-100526), doxycycline (Dox) (Sigma, Cat. No. 33429), ACT-1004–1239 (MCE, Cat. No. HY-142617), TC14012 (MCE, Cat. No. HY-P1102), verteporfin (MCE, Cat. No. HY-B0146), GSK429286A (MCE, Cat. No. HY-11000), Y27632 (MCE, Cat. No. HY-10071), and C3 (Cytoskeleton, Inc., Cat. No. CT03).

Ethidium Homodimer Assay:

Article Title: Targeting the CXCR7 pathway with TC14012 to inhibit endothelial necroptosis and lung cancer metastasis.
Article Snippet: Endothelial necroptosis plays a crucial role in regulating cancer metastasis.. Our previous research demonstrated that TC14012, which is an agonist of CXCR7, exhibits protective effects against endothelial injury.. This study was designed to elucidate the effects of TC14012 on endothelial necroptosis and cancer lung metastasis, along with deciphering the underlying molecular mechanisms.

Recombinant:

Article Title: Crosstalk between purinergic receptor P2Y 11 and chemokine receptor CXCR7 is regulated by CXCR4 in human macrophages
Article Snippet: The ATP analog ATPγS (Sigma Aldrich, St. Louis, MO, USA) and the suramin analog NF340 (Santa Cruz, Dallas, TX, USA) were utilized as P2Y 11 receptor agonist (20 μM) and antagonist (20 μM), respectively. .. Additional reagents employed in this study: the PDE4-selective inhibitor rolipram (10 μM) (Sigma-Aldrich), recombinant IL-1β (0.2 and 2 ng·ml − 1 ) (R&D Systems, Minneapolis, MN, USA), the selective EGFR tyrosine kinase inhibitors AG1478 (10 μM) (Sigma) and erlotinib (20 μM) (Cayman Chemical, Ann Arbor, MI, USA), the CXCR4 chemokine receptor antagonists mavorixafor (AMD 070; 2–10 μM), plerixafor (AMD 3100; 2–10 μM), which is also an allosteric agonist of CXCR7, and TC14012 (2–10 μM), which is also a potent CXCR7 agonist (all from MedChemExpress, Monmouth Junction, NJ, USA), the MEK/ERK inhibitor U0126 (10 μM) (Sigma), the ACKR3 (CXCR7) chemokine receptor agonist VUF11207 (100 nM) (MedChemExpress), the TACE/ADAM17 inhibitor TAPI-1 (20 μM) and the ADAM10 inhibitor GI 254,023 × (10 μM) (both from Tocris, Bristol, UK), a polyclonal goat anti-human HB-EGF antibody (10 μg·ml − 1 ) (AF-259-SP; R&D Systems, Minneapolis, MN, USA), a polyclonal goat anti-human TGFα antibody (10 μg·ml − 1 ) (AB-239-NA; R&D Systems), a polyclonal goat anti-human CXCL12 antibody (10 μg·ml − 1 ) (PA1-20154; Invitrogen / Thermo Fisher Scientific, Waltham, MA, USA), and a monoclonal mouse anti-human CXCL11/I-TAC antibody (10 μg·ml − 1 ) (MAB672; R&D Systems). ..

Transfection:

Article Title: CXCR7 agonist TC14012 improves angiogenic function of endothelial progenitor cells via activating Akt/eNOS pathway and promotes ischemic angiogenesis in diabetic limb ischemia
Article Snippet: For signal test, EPCs were harvested at 10 min after mannitol or HG with or without TC14012 (5 μmol/L, Cayman Chemical Company, Ann Arbor, MI) treatment. .. To confirm the critical role of Akt pathway in mediating the protective effect of TC14012, 200 nmol/L wortmannin (MedChemExpress, Shanghai, China) was used to inhibit the activation of Akt signal. siRNA transfection To knockdown CXCR7 expression in EPCs, siRNAs against human CXCR7 or a Silencer Select Negative Control (GenePharma, Shanghai, China) were transfected into EPCs using GP-transfect-Mate (GenePharma, Shanghai, China). ..

Knockdown:

Article Title: CXCR7 agonist TC14012 improves angiogenic function of endothelial progenitor cells via activating Akt/eNOS pathway and promotes ischemic angiogenesis in diabetic limb ischemia
Article Snippet: For signal test, EPCs were harvested at 10 min after mannitol or HG with or without TC14012 (5 μmol/L, Cayman Chemical Company, Ann Arbor, MI) treatment. .. To confirm the critical role of Akt pathway in mediating the protective effect of TC14012, 200 nmol/L wortmannin (MedChemExpress, Shanghai, China) was used to inhibit the activation of Akt signal. siRNA transfection To knockdown CXCR7 expression in EPCs, siRNAs against human CXCR7 or a Silencer Select Negative Control (GenePharma, Shanghai, China) were transfected into EPCs using GP-transfect-Mate (GenePharma, Shanghai, China). ..

Expressing:

Article Title: CXCR7 agonist TC14012 improves angiogenic function of endothelial progenitor cells via activating Akt/eNOS pathway and promotes ischemic angiogenesis in diabetic limb ischemia
Article Snippet: For signal test, EPCs were harvested at 10 min after mannitol or HG with or without TC14012 (5 μmol/L, Cayman Chemical Company, Ann Arbor, MI) treatment. .. To confirm the critical role of Akt pathway in mediating the protective effect of TC14012, 200 nmol/L wortmannin (MedChemExpress, Shanghai, China) was used to inhibit the activation of Akt signal. siRNA transfection To knockdown CXCR7 expression in EPCs, siRNAs against human CXCR7 or a Silencer Select Negative Control (GenePharma, Shanghai, China) were transfected into EPCs using GP-transfect-Mate (GenePharma, Shanghai, China). ..

Negative Control:

Article Title: CXCR7 agonist TC14012 improves angiogenic function of endothelial progenitor cells via activating Akt/eNOS pathway and promotes ischemic angiogenesis in diabetic limb ischemia
Article Snippet: For signal test, EPCs were harvested at 10 min after mannitol or HG with or without TC14012 (5 μmol/L, Cayman Chemical Company, Ann Arbor, MI) treatment. .. To confirm the critical role of Akt pathway in mediating the protective effect of TC14012, 200 nmol/L wortmannin (MedChemExpress, Shanghai, China) was used to inhibit the activation of Akt signal. siRNA transfection To knockdown CXCR7 expression in EPCs, siRNAs against human CXCR7 or a Silencer Select Negative Control (GenePharma, Shanghai, China) were transfected into EPCs using GP-transfect-Mate (GenePharma, Shanghai, China). ..

Review