recombinant proteins recombinant mouse pd 1 (MedChemExpress)

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MedChemExpress recombinant proteins recombinant mouse pd 1
Recombinant Proteins Recombinant Mouse Pd 1, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hy-p1032/Angiotensin+I/pmc11228665__mmc2-210-93-100
Average 93 stars, based on 2 article reviews

recombinant proteins recombinant mouse pd 1 - by Bioz Stars, 2026-10

93/100 stars

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Recombinant:

Article Title: ANLN promotes head and neck squamous cell carcinoma progression by upregulating PD-L1 via the ERK-MAPK pathway
Article Snippet: Anti-Anillin antibody Abcam Cat#ab211872; RRID: AB_2756815 MEK1/2 antibody Proteintech Cat#11049-1-AP; RRID: AB_2140649 Phospho-MEK1 (Thr292) Antibody Proteintech Cat#28930-1-AP; RRID: AB_2918216 ERK1/2 antibody Proteintech Cat#11257-1-AP; RRID: AB_2139822 Phospho-ERK1/2 (Thr202/Tyr204) antibody Proteintech Cat#28733-1-AP; RRID: AB_2881202 GAPDH antibody Proteintech Cat#60004-1-Ig; RRID: AB_2107436 GZMB antibody Proteintech Cat#13588-1-AP; RRID: AB_2882205 CD8 antibody Proteintech Cat#66868-1-Ig; RRID: AB_2114429 PD-L1 (E1L3N) XP Rabbit Antibody Cell Signaling Technology Cat#13684; RRID: AB_2687655 Anti-rabbit IgG, HRP-linked Antibody Cell Signaling Technology Cat#7074S; RRID: AB_2099233 Anti-mouse IgG, HRP-linked Antibody Cell Signaling Technology Cat#7076S; RRID: AB_330924 .. Mouse tumor tissue This study N/A Human laryngeal carcinoma tissue This study N/A Chemicals, peptides, and recombinant proteins RPMI 1640 Gibco Cat# 12633020 IMDM Gibco Cat# 31980030 DMEM Gibco Cat# 11965092 DMEM/F12 Gibco Cat# A4192001 Opti-MEM Gibco Cat# 31985070 Fetal Bovine Serum Gibco Cat# A5256701 Hydrocortisone MedChemExpress Cat# HY-N0583 Transferrin protein, mouse MedChemExpress Cat# HY-P73453 Insulin-1 protein MedChemExpress Cat# HY-P71805 Triiodothyronine MedChemExpress Cat# HY-A0070A EGF protein, mouse MedChemExpress Cat# HY-P70590 Penicillin/Streptomycin NCM Biotech Cat# C100C5 Penicillin/Streptomycin Biosharp Cat# BL505A TRIzol reagent Invitrogen Cat# 15596018 J urn al Pr e-p roo f EDTA Invitrogen Cat# AM9260G Anti–mouse PD-1 Bio X Cell Cat# BE0146 Mouse IgG2a isotype control Bio X Cell Cat# BE0085 Deoxyribonuclease I Sigma Cat# DN25-1G Opal 7-Color Automation IHC Kit Akoya Biosciences Cat # NEL821001KT DNase/RNase-Free Water Solarbio Cat# R1600 Phosphate Buffered Saline Solarbio Cat# P1020 LipofectamineTM 3000 Transfection Reagent Thermo Fisher Cat# L3000015 Trypsin Thermo Fisher Cat# 25200056 First Strand cDNA Synthesis Kit TOYOBO Cat# FSK-101 SYBR Green Realtime PCR Master Mix-Plus- TOYOBO Cat# QPK-212 Deposited data Raw and analyzed data , see Table S2 This paper N/A Experimental models: Cell lines AMC-HN-8 ATCC RRID: CVCL_5966 FaDu ATCC RRID: CVCL_1218 NOK-SI ATCC RRID: CVCL_BW57 SCC-4 ATCC RRID: CVCL_1684 mEER ATCC RRID: CVCL_B6J2 Tu212 ATCC RRID: CVCL_4915 DOK ATCC RRID: CVCL_1180 Experimental models: Organisms/strains C57BL/6 mice Vital River Laboratory Animal Technology (Beijing, China) N/A .. Primers for quantitative PCR, see Table S3 This paper N/A siRNA/shRNA targeting sequence, see Table S4 This paper N/A Software and algorithms ImageJ software National Institutes of Health N/A FlowJo v10.8.1 software FlowJo LLC N/A

Article Title: PPY-Induced iCAFs Cultivate an Immunosuppressive Microenvironment in Pancreatic Cancer.
Article Snippet: .. RNA Extraction and qRT-PCR: The human or mouse CAFs were collected following treatment with the corresponding recombinant proteins (Human PPY protein (HY-P71062, MCE), Human IL-1α protein (HYP7027, MCE), Mouse PPY protein (RPB265Mu01, Cloud-Clone Corp.), Mouse IL-1α protein (HY-P7072, MCE)) or supernatants for the desig- by co-injecting cancer cells with up-regulated PPY expression and KPC CAFs with down-regulated EGFR expression. ..

Article Title: Glycoengineering-based anti-PD-1-iRGD peptide conjugate boosts antitumor efficacy through T cell engagement
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies aPD-1-(iRGD)2 (CS1003) This paper N/A aPD-1-(iRGD)2 (G4C2) This paper N/A aPD-1-(iRGD)2-Cy5 (CS1003) This paper N/A aPD-1-Cy5 (CS1003) This paper N/A aPD-1 (CS1003) (Li et al., 2021)15 N/A aPD-1 (G4C2) TopAlliance N/A Anti-human IgG-PE Abcam Cat# ab7005, RRID:AB_955489 Anti-human IgG-FITC Abcam Cat# ab6854, RRID:AB_955300 Anti-human CD3-APC Biolegend Cat# 300412, RRID:AB_314065 Anti-human CD3-PerCP Biolegend Cat# 981016, RRID:AB_2876777 Anti-human CD8-FITC Biolegend Cat# 980908, RRID:AB_2888883 Anti-human CD19-PE Biolegend Cat# 982402, RRID:AB_2616905 Anti-human HLA-A2-PE Biolegend Cat# 343305, RRID:AB_1877227 Anti-human PD-1-PE Biolegend Cat# 379209, RRID:AB_2922607 Anti-human CD69-PC7 Biolegend Cat# 310912, RRID:AB_314847 Anti-human Integrin avb5-APC Biolegend Cat# 920011, RRID:AB_2894599 Anti-human NRP-1-PE Biolegend Cat# 354503, RRID:AB_11219194 Anti-human NRP-1 Biolegend BioLegend Cat# 354502, RRID:AB_2564475 Anti-mouse GZMB-AF647 Biolegend BioLegend Cat# 515406, RRID:AB_2294995 Anti-mouse IFNg-APC Biolegend Cat# 505810, RRID:AB_315404 Anti-mouse IFNg-PerCP5.5 Biolegend Cat# 505822, RRID:AB_961361 Anti-mouse CD25-PE eBioscience Cat# 12-0251-83, RRID:AB_465608 Anti-mouse CD69-FITC Biolegend Cat# 104506, RRID:AB_313108 Anti-mouse CD3-FITC Biolegend Cat# 100204, RRID:AB_312660 Anti-mouse CD3e-PC7 Biolegend Cat# 100320, RRID:AB_312685 Anti-mouse CD4-FITC Biolegend Cat# 100406, RRID:AB_312690 Anti-mouse CD4-PerCP5.5 Biolegend Cat# 100434, RRID:AB_893330 Anti-mouse CD4-APC Biolegend Cat# 100412, RRID:AB_312696 Anti-mouse CD4-PE Biolegend Cat# 100408, RRID:AB_312692 Anti-mouse CD8-PE Abcam Cat# ab25498, RRID:AB_470588 Anti-mouse CD8-APC Biolegend Cat# 100712, RRID:AB_312751 Anti-mouse CD8-PerCP Biolegend Cat# 100734, RRID:AB_2075238 Anti-mouse CD8-FITC Biolegend Cat# 100706, RRID:AB_312745 Anti-mouse CD8 Bioxcell Cat# BE0117, RRID:AB_10950145 Anti-mouse PD-1-PE Biolegend Cat# 135206, RRID:AB_1877232 Anti-mouse PD-1-FITC Biolegend Cat# 135213, RRID:AB_10689633 Anti-mouse CD137-PE Biolegend Cat# 106106, RRID:AB_2287565 Anti-mouse Ki67-PC7 Biolegend Cat# 151217, RRID:AB_2910305 Anti-mouse CD39-PerCP Biolegend Cat# 143806, RRID:AB_2563393 Anti-mouse CD45-FITC Biolegend Cat# 103108, RRID:AB_312972 Anti-mouse Foxp3-AF647 Biolegend Cat# 320014, RRID:AB_439750 Anti-mouse Integrin avb5 Biolegend Cat# 153202, RRID:AB_2687270 Anti-mouse Integrin avb5-PE Biolegend Cat# 104106, RRID:AB_2129493 (Continued on next page) e1 Cell Reports Medicine 5, 101590, June 18, 2024 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER Anti-mouse NRP-1-PerCP5.5 Biolegend Cat# 145208, RRID:AB_2562034 Anti-mouse PD-L1-PerCP5.5 Biolegend Cat# 124334, RRID:AB_2629831 Anti-mouse CD11b-FITC Biolegend Cat# 101206, RRID:AB_312788 Anti-mouse CD11b-APC Biolegend Cat# 101212, RRID:AB_312795 Anti-mouse CD11c-FITC Biolegend Cat# 117306, RRID:AB_313775 Anti-mouse F4/80-FITC Biolegend Cat# 123108, RRID:AB_893502 Anti-mouse MHC II-APC Biolegend Cat# 107614, RRID:AB_313329 Anti-mouse CD83-PE Biolegend Cat# 121508, RRID:AB_572015 Anti-mouse CD103-PerCP Biolegend Cat# 121406, RRID:AB_1133989 Anti-mouse CD86-PerCP Biolegend Cat# 105028, RRID:AB_893420 Anti-mouse CD163-PE Biolegend Cat# 156704, RRID:AB_2860724 Anti-mouse Ly6G-PE Biolegend Cat# 127608, RRID:AB_1186104 Anti-mouse Ly6G-PC7 Biolegend Cat# 127618, RRID:AB_1877262 Anti-mouse NRP-1 Biolegend Cat# 145201, RRID:AB_2561840 Chemicals, peptides, and recombinant proteins Recombinant Mouse PD-1 (HEK293, His) MCE HY-P73724 Recombinant human PD-1 (HEK293, His) MCE HY-P7396 Blinatumomab MCE HY-P9963 FTY720 MCE HY-12005 Recombinant mouse IL7 Peprotech #210-07 Recombinant mouse IL15 Peprotech #210-15 Dynabeads for T cell activation/expansion TM Mouse T activator CD3/CD28 Gibco #11453D iRGD, c(CRGDKGPDC) Genescript Biotech Corporation N/A BCN-Cy5 Xi’an Ruixi Biological Technology Co., Ltd N/A N3-iRGD Nanjing Yuan Peptide Biotech Ltd. N/A OVA257-264 peptide Genscript Biotech Corporation N/A GP33 peptide Genscript Biotech Corporation N/A NY-ESO-1157-165 Genscript Biotech Corporation N/A collagenase IV Sigma-Aldrich C4-BIOC DNase I Sigma-Aldrich 04536282001 CFSE eBioscience #65-0850-84 Cell Proliferation Dye eFluorTM 670 eBioscience #65-0840-85 Cell Proliferation Dye eFluorTM 450 eBioscience #65-0842-90 CellTrackerTM Blue Invitrogen #C12881 Deep Red cell tracer Invitrogen #C37608 Zombie AquaTM Fixable Viability Kit Biolegend #423101 Critical commercial assays Cytofix/CytopermTM Fixation/Permeabilization Kit BD Biosciences #554714 Foxp3/transcription factor flow cytometry fixed membrane breaking buffer EBioscience #00-5523-00 GEXSCOPE Single Cell RNA-seq Kit Singleron Biotechnologies N/A Deposited data Mouse CD45+ cells single cell RNA-seq data This paper CNGB database: CNP0005642. ..

Control:

Immunohistochemistry:

Saline:

Transfection:

cDNA Synthesis:

SYBR Green Assay:

Polymerase Chain Reaction:

RNA Extraction:

Expressing:

other:

Article Title: Bioactive equivalent combinatorial components of Xiao-Xu-Ming decoction inhibit the calmodulin-mediated MLCK/MLC axis to attenuate coronary artery spasm.
Article Snippet: Background: Coronary artery spasm (CAS) is a severe pathological disorder with limited treatment options.. Inhibiting vascular smooth muscle cell (VSMC) proliferation has emerged as a promising therapeutic strategy for

Immunopeptidomics:

Activation Assay:

Flow Cytometry:

Membrane:

Single Cell RNA-Seq:

Sequencing:

Article Title: SEMA6B induces macrophage-mediated inflammation and hepatocyte apoptosis in hepatitis B virus-related acute-on-chronic liver failure
Article Snippet: .. Antibodies RRID Company/ Cat Numb Dilution Primary antibodies SEMA6B AB_2783522 Santa Cruz Biotechnology/ Cat# sc390928 1:50/1:500 CD86 AB_869050 Abcam/ Cat# ab53004 1:1000 ALB AB_2673704 Sigma-Aldrich/ Cat# HPA031025 1:2500 Secondary antibodies Goat anti-rabbit IgG (HRP) AB_955447 Abcam/ Cat# ab6721 1:1000 Goat anti-mouse IgG (HRP) AB_955439 Abcam/ Cat# ab6789 1:1000 Goat anti-rabbit IgG (H+L), CFTM 647 AB_2714032 Abcam/ Cat# ab150083 1:200 Goat anti-mouse IgG (H+L), AFTM 488 Invitrogen/ A1101 1:200 Table S4: Primers used in this study Name Sequence Gene ID mGAPDH Forward 5’-CATCACTGCCACCCAGAAGACTG-3’ 14433 Reverse 5’-ATGCCAGTGAGCTTCCCGTTCAG-3’ mSEMA6B Forward 5’-CGCTGTCTTCTCAACTCCTAGC-3’ 20359 Reverse 5’-TCTTCAGGCACTGGTGTCCAGA-3’ mIL-6 Forward 5’-TACCACTTCACAAGTCGGAGGC-3’ 16193 Reverse 5’-CTGCAAGTGCATCATCGTTGTTC-3’ mIL-10 Forward 5’-CGGGAAGACAATAACTGCACCC-3’ 16153 Reverse 5’-CGGTTAGCAGTATGTTGTCCAGC-3’ mIL-1β Forward 5’-TGGACCTTCCAGGATGAGGACA-3’ 16176 Reverse 5’-GTTCATCTCGGAGCCTGTAGTG-3’ mIL-1a Forward 5’-ACGGCTGAGTTTCAGTGAGACC-3’ 16175 Reverse 5’-CACTCTGGTAGGTGTAAGGTGC-3’ mTNF-a Forward 5’-GGTGCCTATGTCTCAGCCTCTT-3’ 21926 Reverse 5’-GCCATAGAACTGATGAGAGGGAG-3’ hSEMA6B Forward 5’- GTCGGAGACAACATCAGCGGTA-3’ 10501 Reverse 5’-GCATCAATGGCTAGGAAGTCGG-3’ hGAPDH Forward 5’- CTCTCTGCTCCTCCTGTTCG-3’ 2597 Reverse 5’- ACGACCAAATCCGTTGACTC-3’ sgRNAmSEMA6B 5’-CACCGCACACTGTAATTGGCGCAGA-3’ 5’-AAAC TCTGCGCCAATTACAGTGTGC-3’ hIL-1β Forward 5’-ATGATGGCTTATTACAGTGGCAA-3’ 3553 Reverse 5’-GTCGGAGATTCGTAGCTGGA-3’ hIL-6 Forward 5’-ACTCACCTCTTCAGAACGAATTG-3’ 3569 Reverse 5’-CCATCTTTGGAAGGTTCAGGTTG-3’ hIL-10 Forward 5’-GACTTTAAGGGTTACCTGGGTTG-3’ 3586 Reverse 5’-TCACATGCGCCTTGATGTCTG-3’ hIL-1α Forward 5’-AGATGCCTGAGATACCCAAAACC -3’ 3552 Reverse 5’-CCAAGCACACCCAGTAGTCT-3’ hTNF-α Forward 5’-GAGGCCAAGCCCTGGTATG -3’ 7124 Reverse 5’-CGGGCCGATTGATCTCAGC-3’ hIL-2 Forward 5’-TACAAGAACCCGAAACTGACTCG-3’ 3558 Reverse 5’-ACATGAAGGTAGTCTCACTGCC-3’ mCCL2 Forward 5’-TTAAAAACCTGGATCGGAACCAA-3’ 20296 Reverse 5’-GCATTAGCTTCAGATTTACGGGT-3’ hCCL2 Forward 5’-CAGCCAGATGCAATCAATGCC-3’ 6347 Reverse 5’-TGGAATCCTGAACCCACTTCT-3’ hICAM1 Forward 5’-ATGCCCAGACATCTGTGTCC-3’ 3383 Reverse 5’-GGGGTCTCTATGCCCAACAA-3’ mICAM1 Forward 5’-GTGATGCTCAGGTATCCATCCA-3’ 15894 Reverse 5’-CACAGTTCTCAAAGCACAGCG-3’ mIL-12 Forward 5’-TGGTTTGCCATCGTTTTGCTG-3’ 16160 Reverse 5’-ACAGGTGAGGTTCACTGTTTCT-3’ hIL-12 Forward 5’-ACCCTGACCATCCAAGTCAAA-3’ 3593 Reverse 5’-TTGGCCTCGCATCTTAGAAAG-3’ msiRNASEMA6B 5’-CACACUUCUACUUCAACGUTT-3’ 5’-ACGUUGAAGUAGAAGUGUGTT-3’ hsiRNASEMA6B SEMA6B Human Pre-designed siRNA Set A (siRNA-1) (MCE, HY-RS12666) NOTE. h, human; m, mouse. ..