Activity Assay:
FLAG-tag:Article Title: Pyroptotic gasdermin exists in Mollusca and is vital to eliminating bacterial infection.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal to 63 His tag Abcam Cat#ab49936; RRID: AB_867459 Mouse monoclonal to mCherry Abcam Cat#ab125096; RRID: AB_11133266 Mouse monoclonal to beta actin Abcam Cat#ab8226; RRID: AB_306371 Mouse monoclonal to FLAG tag Abcam Cat#ab125243; RRID: AB_11001232 Goat anti-mouse IgG H&L (HRP) Abcam Cat#ab205719; RRID: AB_2755049 Mouse anti-HdGSDME polyclonal antibodies This paper N/A Bacterial and virus strains E. coli Trelief 5a Tsingke Biological Technology Cat#TSC01 E. coli Transetta (DE3) TransGen Cat#CD801 Vibrio harveyi Zhang et al.35 N/A Chemicals, peptides, and recombinant proteins PolyJet transfection reagent SignaGen Laboratories Cat#L100688 Lipofectamine 3000 Invitrogen Cat#L3000075 CytoTox 96 Non-Radioactive Cytotoxicity Assay Promega Cat#G1780 Isopropyl-b-D-thiogalactopyranoside Solarbio Life Sciences Cat#367-93-1 Ni-NTA agarose Qiagen Cat#30230 complete Freund’s adjuvant Sigma-Aldrich Cat#F5881 incomplete Freund’s adjuvant Sigma-Aldrich Cat#F5506 Protein G Sepharose GE Healthcare Cat#17-0618-01 HsCASP1 Enzo Life Sciences Cat#ALX-201-056 HsCASP2 Enzo Life Sciences Cat#ALX-201-057 HsCASP3 Enzo Life Sciences Cat#ALX-201-059 HsCASP6 Enzo Life Sciences Cat#ALX-201-060 HsCASP7 Enzo Life Sciences Cat#ALX-201-061 HsCASP8 Enzo Life Sciences Cat#ALX-201-062 HsCASP9 Enzo Life Sciences Cat#ALX-201-047 Sytox green Invitrogen Cat#S34860 Propidium iodide Invitrogen Cat#P1304MP; CAS:25535-16-4 Ac-YVAD-rNA Enzo Life Sciences Cat#ALX-260-026; CAS:149231-66-3 Ac-VDQQD-rNA Sigma-Aldrich Cat#SCP0105 Ac-DEVD-rNA Enzo Life Sciences Cat#ALX-260-033; CAS:189950-66-1 Ac-VEID-rNA Enzo Life Sciences Cat#ALX-260-063 Ac-IETD-rNA Enzo Life Sciences Cat#ALX-260-045 Ac-LEHD-rNA Enzo Life Sciences Cat#ALX-260-081 Ac-DEVD-AFC MedChem Express Cat#HY-P1005; CAS:201608-14-2 Z-DEVD-FMK MedChem Express Cat#HY-12466; CAS:210344-95-9 HdCASP3 This paper N/A HdGSDME This paper N/A 16:0-18:1 PE Avanti Polar Lipids Cat#850757 16:0-18:1 PC Avanti Polar Lipids Cat#850457 (Continued on next page) Cell Reports 42, 112414, May 30, 2023 13 .. REAGENT or RESOURCE SOURCE IDENTIFIER Cardiolipin (bovine heart) Sigma-Aldrich Cat#C1649 Dipicolinic acid Sigma-Aldrich Cat#P63808; CAS: 499-83-2 Terbium (III) chloride Sigma-Aldrich Cat#451304; CAS:10042-88-3 Critical commercial assays Histochemistry Wuhan Servicebio Technology N/A Cell-free E. coli Protein Synthesis System New England Biolabs Cat#E5360 Experimental models: Cell lines HEK293T ATCC Cat#CRL-3216 Experimental models: Organisms/strains Haliotis discus A local farm in Weihai, Shandong Province N/A Oligonucleotides Primers for this paper, see Table S1 This paper N/A Recombinant DNA pmCherry-N1-mollusk GSDMs This paper N/A pmCherry-N1-HdGSDME variants (full length, truncates, and mutants) This paper N/A pEGFP-N1-HdCASP3 This paper N/A pET-32a (+)-HdGSDME variants (full length and mutants) This paper N/A pCAGGS-FLAG-HdGSDME wild type and mutants This paper N/A pET-30a (+)-mollusk GSDMs This paper N/A pET-30a (+)-HdCASP3 This paper N/A Software and algorithms MEGA 7.0 Kumar et al.36 http://megasoftware.net/ Graphpad Prism GraphPad Software http://www.graphpad.com/ SWISS-MODEL Waterhouse et al.37 https://swissmodel.expasy.org/ CLUSTALW Thompson et al.38 https://www.genome.jp/tools-bin/clustalw ESPript 3.0 Robert and Gouet.39 http://espript.ibcp.fr/ESPript/cgi-bin/ESPript.cgi
Virus:Article Title: Pyroptotic gasdermin exists in Mollusca and is vital to eliminating bacterial infection.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal to 63 His tag Abcam Cat#ab49936; RRID: AB_867459 Mouse monoclonal to mCherry Abcam Cat#ab125096; RRID: AB_11133266 Mouse monoclonal to beta actin Abcam Cat#ab8226; RRID: AB_306371 Mouse monoclonal to FLAG tag Abcam Cat#ab125243; RRID: AB_11001232 Goat anti-mouse IgG H&L (HRP) Abcam Cat#ab205719; RRID: AB_2755049 Mouse anti-HdGSDME polyclonal antibodies This paper N/A Bacterial and virus strains E. coli Trelief 5a Tsingke Biological Technology Cat#TSC01 E. coli Transetta (DE3) TransGen Cat#CD801 Vibrio harveyi Zhang et al.35 N/A Chemicals, peptides, and recombinant proteins PolyJet transfection reagent SignaGen Laboratories Cat#L100688 Lipofectamine 3000 Invitrogen Cat#L3000075 CytoTox 96 Non-Radioactive Cytotoxicity Assay Promega Cat#G1780 Isopropyl-b-D-thiogalactopyranoside Solarbio Life Sciences Cat#367-93-1 Ni-NTA agarose Qiagen Cat#30230 complete Freund’s adjuvant Sigma-Aldrich Cat#F5881 incomplete Freund’s adjuvant Sigma-Aldrich Cat#F5506 Protein G Sepharose GE Healthcare Cat#17-0618-01 HsCASP1 Enzo Life Sciences Cat#ALX-201-056 HsCASP2 Enzo Life Sciences Cat#ALX-201-057 HsCASP3 Enzo Life Sciences Cat#ALX-201-059 HsCASP6 Enzo Life Sciences Cat#ALX-201-060 HsCASP7 Enzo Life Sciences Cat#ALX-201-061 HsCASP8 Enzo Life Sciences Cat#ALX-201-062 HsCASP9 Enzo Life Sciences Cat#ALX-201-047 Sytox green Invitrogen Cat#S34860 Propidium iodide Invitrogen Cat#P1304MP; CAS:25535-16-4 Ac-YVAD-rNA Enzo Life Sciences Cat#ALX-260-026; CAS:149231-66-3 Ac-VDQQD-rNA Sigma-Aldrich Cat#SCP0105 Ac-DEVD-rNA Enzo Life Sciences Cat#ALX-260-033; CAS:189950-66-1 Ac-VEID-rNA Enzo Life Sciences Cat#ALX-260-063 Ac-IETD-rNA Enzo Life Sciences Cat#ALX-260-045 Ac-LEHD-rNA Enzo Life Sciences Cat#ALX-260-081 Ac-DEVD-AFC MedChem Express Cat#HY-P1005; CAS:201608-14-2 Z-DEVD-FMK MedChem Express Cat#HY-12466; CAS:210344-95-9 HdCASP3 This paper N/A HdGSDME This paper N/A 16:0-18:1 PE Avanti Polar Lipids Cat#850757 16:0-18:1 PC Avanti Polar Lipids Cat#850457 (Continued on next page) Cell Reports 42, 112414, May 30, 2023 13 .. REAGENT or RESOURCE SOURCE IDENTIFIER Cardiolipin (bovine heart) Sigma-Aldrich Cat#C1649 Dipicolinic acid Sigma-Aldrich Cat#P63808; CAS: 499-83-2 Terbium (III) chloride Sigma-Aldrich Cat#451304; CAS:10042-88-3 Critical commercial assays Histochemistry Wuhan Servicebio Technology N/A Cell-free E. coli Protein Synthesis System New England Biolabs Cat#E5360 Experimental models: Cell lines HEK293T ATCC Cat#CRL-3216 Experimental models: Organisms/strains Haliotis discus A local farm in Weihai, Shandong Province N/A Oligonucleotides Primers for this paper, see Table S1 This paper N/A Recombinant DNA pmCherry-N1-mollusk GSDMs This paper N/A pmCherry-N1-HdGSDME variants (full length, truncates, and mutants) This paper N/A pEGFP-N1-HdCASP3 This paper N/A pET-32a (+)-HdGSDME variants (full length and mutants) This paper N/A pCAGGS-FLAG-HdGSDME wild type and mutants This paper N/A pET-30a (+)-mollusk GSDMs This paper N/A pET-30a (+)-HdCASP3 This paper N/A Software and algorithms MEGA 7.0 Kumar et al.36 http://megasoftware.net/ Graphpad Prism GraphPad Software http://www.graphpad.com/ SWISS-MODEL Waterhouse et al.37 https://swissmodel.expasy.org/ CLUSTALW Thompson et al.38 https://www.genome.jp/tools-bin/clustalw ESPript 3.0 Robert and Gouet.39 http://espript.ibcp.fr/ESPript/cgi-bin/ESPript.cgi
Recombinant:Article Title: Pyroptotic gasdermin exists in Mollusca and is vital to eliminating bacterial infection.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal to 63 His tag Abcam Cat#ab49936; RRID: AB_867459 Mouse monoclonal to mCherry Abcam Cat#ab125096; RRID: AB_11133266 Mouse monoclonal to beta actin Abcam Cat#ab8226; RRID: AB_306371 Mouse monoclonal to FLAG tag Abcam Cat#ab125243; RRID: AB_11001232 Goat anti-mouse IgG H&L (HRP) Abcam Cat#ab205719; RRID: AB_2755049 Mouse anti-HdGSDME polyclonal antibodies This paper N/A Bacterial and virus strains E. coli Trelief 5a Tsingke Biological Technology Cat#TSC01 E. coli Transetta (DE3) TransGen Cat#CD801 Vibrio harveyi Zhang et al.35 N/A Chemicals, peptides, and recombinant proteins PolyJet transfection reagent SignaGen Laboratories Cat#L100688 Lipofectamine 3000 Invitrogen Cat#L3000075 CytoTox 96 Non-Radioactive Cytotoxicity Assay Promega Cat#G1780 Isopropyl-b-D-thiogalactopyranoside Solarbio Life Sciences Cat#367-93-1 Ni-NTA agarose Qiagen Cat#30230 complete Freund’s adjuvant Sigma-Aldrich Cat#F5881 incomplete Freund’s adjuvant Sigma-Aldrich Cat#F5506 Protein G Sepharose GE Healthcare Cat#17-0618-01 HsCASP1 Enzo Life Sciences Cat#ALX-201-056 HsCASP2 Enzo Life Sciences Cat#ALX-201-057 HsCASP3 Enzo Life Sciences Cat#ALX-201-059 HsCASP6 Enzo Life Sciences Cat#ALX-201-060 HsCASP7 Enzo Life Sciences Cat#ALX-201-061 HsCASP8 Enzo Life Sciences Cat#ALX-201-062 HsCASP9 Enzo Life Sciences Cat#ALX-201-047 Sytox green Invitrogen Cat#S34860 Propidium iodide Invitrogen Cat#P1304MP; CAS:25535-16-4 Ac-YVAD-rNA Enzo Life Sciences Cat#ALX-260-026; CAS:149231-66-3 Ac-VDQQD-rNA Sigma-Aldrich Cat#SCP0105 Ac-DEVD-rNA Enzo Life Sciences Cat#ALX-260-033; CAS:189950-66-1 Ac-VEID-rNA Enzo Life Sciences Cat#ALX-260-063 Ac-IETD-rNA Enzo Life Sciences Cat#ALX-260-045 Ac-LEHD-rNA Enzo Life Sciences Cat#ALX-260-081 Ac-DEVD-AFC MedChem Express Cat#HY-P1005; CAS:201608-14-2 Z-DEVD-FMK MedChem Express Cat#HY-12466; CAS:210344-95-9 HdCASP3 This paper N/A HdGSDME This paper N/A 16:0-18:1 PE Avanti Polar Lipids Cat#850757 16:0-18:1 PC Avanti Polar Lipids Cat#850457 (Continued on next page) Cell Reports 42, 112414, May 30, 2023 13 .. REAGENT or RESOURCE SOURCE IDENTIFIER Cardiolipin (bovine heart) Sigma-Aldrich Cat#C1649 Dipicolinic acid Sigma-Aldrich Cat#P63808; CAS: 499-83-2 Terbium (III) chloride Sigma-Aldrich Cat#451304; CAS:10042-88-3 Critical commercial assays Histochemistry Wuhan Servicebio Technology N/A Cell-free E. coli Protein Synthesis System New England Biolabs Cat#E5360 Experimental models: Cell lines HEK293T ATCC Cat#CRL-3216 Experimental models: Organisms/strains Haliotis discus A local farm in Weihai, Shandong Province N/A Oligonucleotides Primers for this paper, see Table S1 This paper N/A Recombinant DNA pmCherry-N1-mollusk GSDMs This paper N/A pmCherry-N1-HdGSDME variants (full length, truncates, and mutants) This paper N/A pEGFP-N1-HdCASP3 This paper N/A pET-32a (+)-HdGSDME variants (full length and mutants) This paper N/A pCAGGS-FLAG-HdGSDME wild type and mutants This paper N/A pET-30a (+)-mollusk GSDMs This paper N/A pET-30a (+)-HdCASP3 This paper N/A Software and algorithms MEGA 7.0 Kumar et al.36 http://megasoftware.net/ Graphpad Prism GraphPad Software http://www.graphpad.com/ SWISS-MODEL Waterhouse et al.37 https://swissmodel.expasy.org/ CLUSTALW Thompson et al.38 https://www.genome.jp/tools-bin/clustalw ESPript 3.0 Robert and Gouet.39 http://espript.ibcp.fr/ESPript/cgi-bin/ESPript.cgi
Transfection:Article Title: Pyroptotic gasdermin exists in Mollusca and is vital to eliminating bacterial infection.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal to 63 His tag Abcam Cat#ab49936; RRID: AB_867459 Mouse monoclonal to mCherry Abcam Cat#ab125096; RRID: AB_11133266 Mouse monoclonal to beta actin Abcam Cat#ab8226; RRID: AB_306371 Mouse monoclonal to FLAG tag Abcam Cat#ab125243; RRID: AB_11001232 Goat anti-mouse IgG H&L (HRP) Abcam Cat#ab205719; RRID: AB_2755049 Mouse anti-HdGSDME polyclonal antibodies This paper N/A Bacterial and virus strains E. coli Trelief 5a Tsingke Biological Technology Cat#TSC01 E. coli Transetta (DE3) TransGen Cat#CD801 Vibrio harveyi Zhang et al.35 N/A Chemicals, peptides, and recombinant proteins PolyJet transfection reagent SignaGen Laboratories Cat#L100688 Lipofectamine 3000 Invitrogen Cat#L3000075 CytoTox 96 Non-Radioactive Cytotoxicity Assay Promega Cat#G1780 Isopropyl-b-D-thiogalactopyranoside Solarbio Life Sciences Cat#367-93-1 Ni-NTA agarose Qiagen Cat#30230 complete Freund’s adjuvant Sigma-Aldrich Cat#F5881 incomplete Freund’s adjuvant Sigma-Aldrich Cat#F5506 Protein G Sepharose GE Healthcare Cat#17-0618-01 HsCASP1 Enzo Life Sciences Cat#ALX-201-056 HsCASP2 Enzo Life Sciences Cat#ALX-201-057 HsCASP3 Enzo Life Sciences Cat#ALX-201-059 HsCASP6 Enzo Life Sciences Cat#ALX-201-060 HsCASP7 Enzo Life Sciences Cat#ALX-201-061 HsCASP8 Enzo Life Sciences Cat#ALX-201-062 HsCASP9 Enzo Life Sciences Cat#ALX-201-047 Sytox green Invitrogen Cat#S34860 Propidium iodide Invitrogen Cat#P1304MP; CAS:25535-16-4 Ac-YVAD-rNA Enzo Life Sciences Cat#ALX-260-026; CAS:149231-66-3 Ac-VDQQD-rNA Sigma-Aldrich Cat#SCP0105 Ac-DEVD-rNA Enzo Life Sciences Cat#ALX-260-033; CAS:189950-66-1 Ac-VEID-rNA Enzo Life Sciences Cat#ALX-260-063 Ac-IETD-rNA Enzo Life Sciences Cat#ALX-260-045 Ac-LEHD-rNA Enzo Life Sciences Cat#ALX-260-081 Ac-DEVD-AFC MedChem Express Cat#HY-P1005; CAS:201608-14-2 Z-DEVD-FMK MedChem Express Cat#HY-12466; CAS:210344-95-9 HdCASP3 This paper N/A HdGSDME This paper N/A 16:0-18:1 PE Avanti Polar Lipids Cat#850757 16:0-18:1 PC Avanti Polar Lipids Cat#850457 (Continued on next page) Cell Reports 42, 112414, May 30, 2023 13 .. REAGENT or RESOURCE SOURCE IDENTIFIER Cardiolipin (bovine heart) Sigma-Aldrich Cat#C1649 Dipicolinic acid Sigma-Aldrich Cat#P63808; CAS: 499-83-2 Terbium (III) chloride Sigma-Aldrich Cat#451304; CAS:10042-88-3 Critical commercial assays Histochemistry Wuhan Servicebio Technology N/A Cell-free E. coli Protein Synthesis System New England Biolabs Cat#E5360 Experimental models: Cell lines HEK293T ATCC Cat#CRL-3216 Experimental models: Organisms/strains Haliotis discus A local farm in Weihai, Shandong Province N/A Oligonucleotides Primers for this paper, see Table S1 This paper N/A Recombinant DNA pmCherry-N1-mollusk GSDMs This paper N/A pmCherry-N1-HdGSDME variants (full length, truncates, and mutants) This paper N/A pEGFP-N1-HdCASP3 This paper N/A pET-32a (+)-HdGSDME variants (full length and mutants) This paper N/A pCAGGS-FLAG-HdGSDME wild type and mutants This paper N/A pET-30a (+)-mollusk GSDMs This paper N/A pET-30a (+)-HdCASP3 This paper N/A Software and algorithms MEGA 7.0 Kumar et al.36 http://megasoftware.net/ Graphpad Prism GraphPad Software http://www.graphpad.com/ SWISS-MODEL Waterhouse et al.37 https://swissmodel.expasy.org/ CLUSTALW Thompson et al.38 https://www.genome.jp/tools-bin/clustalw ESPript 3.0 Robert and Gouet.39 http://espript.ibcp.fr/ESPript/cgi-bin/ESPript.cgi
Cytotoxicity Assay:Article Title: Pyroptotic gasdermin exists in Mollusca and is vital to eliminating bacterial infection.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal to 63 His tag Abcam Cat#ab49936; RRID: AB_867459 Mouse monoclonal to mCherry Abcam Cat#ab125096; RRID: AB_11133266 Mouse monoclonal to beta actin Abcam Cat#ab8226; RRID: AB_306371 Mouse monoclonal to FLAG tag Abcam Cat#ab125243; RRID: AB_11001232 Goat anti-mouse IgG H&L (HRP) Abcam Cat#ab205719; RRID: AB_2755049 Mouse anti-HdGSDME polyclonal antibodies This paper N/A Bacterial and virus strains E. coli Trelief 5a Tsingke Biological Technology Cat#TSC01 E. coli Transetta (DE3) TransGen Cat#CD801 Vibrio harveyi Zhang et al.35 N/A Chemicals, peptides, and recombinant proteins PolyJet transfection reagent SignaGen Laboratories Cat#L100688 Lipofectamine 3000 Invitrogen Cat#L3000075 CytoTox 96 Non-Radioactive Cytotoxicity Assay Promega Cat#G1780 Isopropyl-b-D-thiogalactopyranoside Solarbio Life Sciences Cat#367-93-1 Ni-NTA agarose Qiagen Cat#30230 complete Freund’s adjuvant Sigma-Aldrich Cat#F5881 incomplete Freund’s adjuvant Sigma-Aldrich Cat#F5506 Protein G Sepharose GE Healthcare Cat#17-0618-01 HsCASP1 Enzo Life Sciences Cat#ALX-201-056 HsCASP2 Enzo Life Sciences Cat#ALX-201-057 HsCASP3 Enzo Life Sciences Cat#ALX-201-059 HsCASP6 Enzo Life Sciences Cat#ALX-201-060 HsCASP7 Enzo Life Sciences Cat#ALX-201-061 HsCASP8 Enzo Life Sciences Cat#ALX-201-062 HsCASP9 Enzo Life Sciences Cat#ALX-201-047 Sytox green Invitrogen Cat#S34860 Propidium iodide Invitrogen Cat#P1304MP; CAS:25535-16-4 Ac-YVAD-rNA Enzo Life Sciences Cat#ALX-260-026; CAS:149231-66-3 Ac-VDQQD-rNA Sigma-Aldrich Cat#SCP0105 Ac-DEVD-rNA Enzo Life Sciences Cat#ALX-260-033; CAS:189950-66-1 Ac-VEID-rNA Enzo Life Sciences Cat#ALX-260-063 Ac-IETD-rNA Enzo Life Sciences Cat#ALX-260-045 Ac-LEHD-rNA Enzo Life Sciences Cat#ALX-260-081 Ac-DEVD-AFC MedChem Express Cat#HY-P1005; CAS:201608-14-2 Z-DEVD-FMK MedChem Express Cat#HY-12466; CAS:210344-95-9 HdCASP3 This paper N/A HdGSDME This paper N/A 16:0-18:1 PE Avanti Polar Lipids Cat#850757 16:0-18:1 PC Avanti Polar Lipids Cat#850457 (Continued on next page) Cell Reports 42, 112414, May 30, 2023 13 .. REAGENT or RESOURCE SOURCE IDENTIFIER Cardiolipin (bovine heart) Sigma-Aldrich Cat#C1649 Dipicolinic acid Sigma-Aldrich Cat#P63808; CAS: 499-83-2 Terbium (III) chloride Sigma-Aldrich Cat#451304; CAS:10042-88-3 Critical commercial assays Histochemistry Wuhan Servicebio Technology N/A Cell-free E. coli Protein Synthesis System New England Biolabs Cat#E5360 Experimental models: Cell lines HEK293T ATCC Cat#CRL-3216 Experimental models: Organisms/strains Haliotis discus A local farm in Weihai, Shandong Province N/A Oligonucleotides Primers for this paper, see Table S1 This paper N/A Recombinant DNA pmCherry-N1-mollusk GSDMs This paper N/A pmCherry-N1-HdGSDME variants (full length, truncates, and mutants) This paper N/A pEGFP-N1-HdCASP3 This paper N/A pET-32a (+)-HdGSDME variants (full length and mutants) This paper N/A pCAGGS-FLAG-HdGSDME wild type and mutants This paper N/A pET-30a (+)-mollusk GSDMs This paper N/A pET-30a (+)-HdCASP3 This paper N/A Software and algorithms MEGA 7.0 Kumar et al.36 http://megasoftware.net/ Graphpad Prism GraphPad Software http://www.graphpad.com/ SWISS-MODEL Waterhouse et al.37 https://swissmodel.expasy.org/ CLUSTALW Thompson et al.38 https://www.genome.jp/tools-bin/clustalw ESPript 3.0 Robert and Gouet.39 http://espript.ibcp.fr/ESPript/cgi-bin/ESPript.cgi
Adjuvant:Article Title: Pyroptotic gasdermin exists in Mollusca and is vital to eliminating bacterial infection.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal to 63 His tag Abcam Cat#ab49936; RRID: AB_867459 Mouse monoclonal to mCherry Abcam Cat#ab125096; RRID: AB_11133266 Mouse monoclonal to beta actin Abcam Cat#ab8226; RRID: AB_306371 Mouse monoclonal to FLAG tag Abcam Cat#ab125243; RRID: AB_11001232 Goat anti-mouse IgG H&L (HRP) Abcam Cat#ab205719; RRID: AB_2755049 Mouse anti-HdGSDME polyclonal antibodies This paper N/A Bacterial and virus strains E. coli Trelief 5a Tsingke Biological Technology Cat#TSC01 E. coli Transetta (DE3) TransGen Cat#CD801 Vibrio harveyi Zhang et al.35 N/A Chemicals, peptides, and recombinant proteins PolyJet transfection reagent SignaGen Laboratories Cat#L100688 Lipofectamine 3000 Invitrogen Cat#L3000075 CytoTox 96 Non-Radioactive Cytotoxicity Assay Promega Cat#G1780 Isopropyl-b-D-thiogalactopyranoside Solarbio Life Sciences Cat#367-93-1 Ni-NTA agarose Qiagen Cat#30230 complete Freund’s adjuvant Sigma-Aldrich Cat#F5881 incomplete Freund’s adjuvant Sigma-Aldrich Cat#F5506 Protein G Sepharose GE Healthcare Cat#17-0618-01 HsCASP1 Enzo Life Sciences Cat#ALX-201-056 HsCASP2 Enzo Life Sciences Cat#ALX-201-057 HsCASP3 Enzo Life Sciences Cat#ALX-201-059 HsCASP6 Enzo Life Sciences Cat#ALX-201-060 HsCASP7 Enzo Life Sciences Cat#ALX-201-061 HsCASP8 Enzo Life Sciences Cat#ALX-201-062 HsCASP9 Enzo Life Sciences Cat#ALX-201-047 Sytox green Invitrogen Cat#S34860 Propidium iodide Invitrogen Cat#P1304MP; CAS:25535-16-4 Ac-YVAD-rNA Enzo Life Sciences Cat#ALX-260-026; CAS:149231-66-3 Ac-VDQQD-rNA Sigma-Aldrich Cat#SCP0105 Ac-DEVD-rNA Enzo Life Sciences Cat#ALX-260-033; CAS:189950-66-1 Ac-VEID-rNA Enzo Life Sciences Cat#ALX-260-063 Ac-IETD-rNA Enzo Life Sciences Cat#ALX-260-045 Ac-LEHD-rNA Enzo Life Sciences Cat#ALX-260-081 Ac-DEVD-AFC MedChem Express Cat#HY-P1005; CAS:201608-14-2 Z-DEVD-FMK MedChem Express Cat#HY-12466; CAS:210344-95-9 HdCASP3 This paper N/A HdGSDME This paper N/A 16:0-18:1 PE Avanti Polar Lipids Cat#850757 16:0-18:1 PC Avanti Polar Lipids Cat#850457 (Continued on next page) Cell Reports 42, 112414, May 30, 2023 13 .. REAGENT or RESOURCE SOURCE IDENTIFIER Cardiolipin (bovine heart) Sigma-Aldrich Cat#C1649 Dipicolinic acid Sigma-Aldrich Cat#P63808; CAS: 499-83-2 Terbium (III) chloride Sigma-Aldrich Cat#451304; CAS:10042-88-3 Critical commercial assays Histochemistry Wuhan Servicebio Technology N/A Cell-free E. coli Protein Synthesis System New England Biolabs Cat#E5360 Experimental models: Cell lines HEK293T ATCC Cat#CRL-3216 Experimental models: Organisms/strains Haliotis discus A local farm in Weihai, Shandong Province N/A Oligonucleotides Primers for this paper, see Table S1 This paper N/A Recombinant DNA pmCherry-N1-mollusk GSDMs This paper N/A pmCherry-N1-HdGSDME variants (full length, truncates, and mutants) This paper N/A pEGFP-N1-HdCASP3 This paper N/A pET-32a (+)-HdGSDME variants (full length and mutants) This paper N/A pCAGGS-FLAG-HdGSDME wild type and mutants This paper N/A pET-30a (+)-mollusk GSDMs This paper N/A pET-30a (+)-HdCASP3 This paper N/A Software and algorithms MEGA 7.0 Kumar et al.36 http://megasoftware.net/ Graphpad Prism GraphPad Software http://www.graphpad.com/ SWISS-MODEL Waterhouse et al.37 https://swissmodel.expasy.org/ CLUSTALW Thompson et al.38 https://www.genome.jp/tools-bin/clustalw ESPript 3.0 Robert and Gouet.39 http://espript.ibcp.fr/ESPript/cgi-bin/ESPript.cgi
other:Article Title: Peptidomimetic Analogues Act as Effective Inhibitors against SARS-CoV-2 by Blocking the Function of Cathepsin L.
Article Snippet: Cathepsin L (CatL) is a promising antiviral drug target of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) as an important protease for cleaving the SARS-CoV-2 spike protein and enhancing viral entry to cells.. We identified a tripeptide aldehyde candidate, D1−1, which exhibited inhibitory effects against SARS-CoV-2 in Vero E6 cells.. The protease screening analysis and protein pull-down assays demonstrated the direct binding of D1−1 to CatL.
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