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ac devd amc  (MedChemExpress)


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    MedChemExpress ac devd amc
    Ac Devd Amc, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hy-p1003/Ac-DEVD-AMC/pm31392992-93-20-21
    Average 94 stars, based on 9 article reviews
    ac devd amc - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Activity Assay:

    Article Title: TNF-α synergises with IFN-γ to induce caspase-8-JAK1/2-STAT1-dependent death of intestinal epithelial cells
    Article Snippet: For detection of SYTOX Green cell death signal, cells were continuously incubated with a cell-impermeable SYTOX Green dye (2.5 μM, S7020, Thermo Fischer), whose fluorescent intensity was measured at 485/530 nm as previously described [ ]. .. For detection of caspase-3 activity, cells were continuously incubated with Ac-DEVD-AMC (10 μM, HY-P1003, Medchem Express), whose fluorescent intensity was measured at 330/460 nm as previously described [ ]. .. Both SYTOX Green cell death signal and caspase-3 activity were normalised to the total DNA fluorescence [ ] measured at 330/460 nm by incubating cells for 30 min with Hoechst 33342 (10 μg/ml in PBS, H3570, Thermo Fischer).

    Article Title: TNF-α synergises with IFN-γ to induce caspase-8-JAK1/2-STAT1-dependent death of intestinal epithelial cells.
    Article Snippet: For detection of SYTOX Green cell death signal, cells were continuously incubated with a cellimpermeable SYTOX Green dye (2.5 μM, S7020, Thermo Fischer), whose fluorescent intensity was measured at 485/530 nm as previously described [86]. .. For detection of caspase-3 activity, cells were continuously incubated with Ac-DEVD-AMC (10 μM, HY-P1003, Medchem Express), whose fluorescent intensity was measured at 330/460 nm as previously described [86]. .. Both SYTOX Green cell death signal and caspase-3 activity were normalised to the total DNA fluorescence [87] measured at 330/460 nm by incubating cells for 30min with Hoechst 33342 (10 μg/ml in PBS, H3570, Thermo Fischer).

    Incubation:

    Article Title: TNF-α synergises with IFN-γ to induce caspase-8-JAK1/2-STAT1-dependent death of intestinal epithelial cells
    Article Snippet: For detection of SYTOX Green cell death signal, cells were continuously incubated with a cell-impermeable SYTOX Green dye (2.5 μM, S7020, Thermo Fischer), whose fluorescent intensity was measured at 485/530 nm as previously described [ ]. .. For detection of caspase-3 activity, cells were continuously incubated with Ac-DEVD-AMC (10 μM, HY-P1003, Medchem Express), whose fluorescent intensity was measured at 330/460 nm as previously described [ ]. .. Both SYTOX Green cell death signal and caspase-3 activity were normalised to the total DNA fluorescence [ ] measured at 330/460 nm by incubating cells for 30 min with Hoechst 33342 (10 μg/ml in PBS, H3570, Thermo Fischer).

    Article Title: TNF-α synergises with IFN-γ to induce caspase-8-JAK1/2-STAT1-dependent death of intestinal epithelial cells.
    Article Snippet: For detection of SYTOX Green cell death signal, cells were continuously incubated with a cellimpermeable SYTOX Green dye (2.5 μM, S7020, Thermo Fischer), whose fluorescent intensity was measured at 485/530 nm as previously described [86]. .. For detection of caspase-3 activity, cells were continuously incubated with Ac-DEVD-AMC (10 μM, HY-P1003, Medchem Express), whose fluorescent intensity was measured at 330/460 nm as previously described [86]. .. Both SYTOX Green cell death signal and caspase-3 activity were normalised to the total DNA fluorescence [87] measured at 330/460 nm by incubating cells for 30min with Hoechst 33342 (10 μg/ml in PBS, H3570, Thermo Fischer).

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    Combining sorafenib with spermine or sphingosine produces synergistic apoptotic effects in hepatocellular carcinoma through cell-cycle arrest. (A) A flow cytometry analysis was performed on SK-Hep-1 cells treated with sorafenib (Sora), spermine (Sper), and/or sphingosine (Sphi). (B) The percentage of cells in each cell-cycle phase was measured by flow cytometry, and the population of cells was plotted. SubG1, red; G1, blue; S, yellow; G2/M, green. (C) The percentage of cells in subG1 fraction was plotted. (D) Immunoblot analyses were performed on cells treated with sorafenib (Sora), spermine (Sper), and/or sphingosine (Sphi), either individually or in combination, using specific antibodies against cyclin A, cyclin E1, cyclin D1, p21 WAF1 , and β-actin. The relative band intensities were plotted. * p < 0.05; ** p < 0.01; *** p < 0.001 compared with control. (E) Immunofluorescence staining was performed in cells after single or combination treatment with sorafenib (Sora), spermine (Sper), and/or sphingosine (Sphi). Cleaved caspase-3 (green), α-tubulin (red), and DNA (DAPI, blue) are displayed. n>3000. The cell images were collected and evaluated with a confocal microscope FW3000 (Olympus; Tokyo, Japan). Scale bar, 20 μm. ( F ) The population of cleaved caspase-3 (green fluorescence)-positive cells was quantified. n>3000. * p < 0.05; ** p < 0.01; *** p < 0.001. Data are presented as the mean ± SD. ( G-H ) SK-Hep-1 cells were treated with sorafenib with and without spermine ( G ), or sorafenib with and without sphingosine ( H ) for 48 hours. Then the relative caspase-3 activity was <t>measured</t> <t>with</t> <t>Ac-DEVD-AMC</t> substrate and plotted. * p < 0.05; ** p < 0.01; *** p < 0.001 compared with control. Data are presented as the mean ± SD.
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    Combining sorafenib with spermine or sphingosine produces synergistic apoptotic effects in hepatocellular carcinoma through cell-cycle arrest. (A) A flow cytometry analysis was performed on SK-Hep-1 cells treated with sorafenib (Sora), spermine (Sper), and/or sphingosine (Sphi). (B) The percentage of cells in each cell-cycle phase was measured by flow cytometry, and the population of cells was plotted. SubG1, red; G1, blue; S, yellow; G2/M, green. (C) The percentage of cells in subG1 fraction was plotted. (D) Immunoblot analyses were performed on cells treated with sorafenib (Sora), spermine (Sper), and/or sphingosine (Sphi), either individually or in combination, using specific antibodies against cyclin A, cyclin E1, cyclin D1, p21 WAF1 , and β-actin. The relative band intensities were plotted. * p < 0.05; ** p < 0.01; *** p < 0.001 compared with control. (E) Immunofluorescence staining was performed in cells after single or combination treatment with sorafenib (Sora), spermine (Sper), and/or sphingosine (Sphi). Cleaved caspase-3 (green), α-tubulin (red), and DNA (DAPI, blue) are displayed. n>3000. The cell images were collected and evaluated with a confocal microscope FW3000 (Olympus; Tokyo, Japan). Scale bar, 20 μm. ( F ) The population of cleaved caspase-3 (green fluorescence)-positive cells was quantified. n>3000. * p < 0.05; ** p < 0.01; *** p < 0.001. Data are presented as the mean ± SD. ( G-H ) SK-Hep-1 cells were treated with sorafenib with and without spermine ( G ), or sorafenib with and without sphingosine ( H ) for 48 hours. Then the relative caspase-3 activity was measured with Ac-DEVD-AMC substrate and plotted. * p < 0.05; ** p < 0.01; *** p < 0.001 compared with control. Data are presented as the mean ± SD.

    Journal: International Journal of Biological Sciences

    Article Title: Combining sorafenib with spermine and sphingosine synergistically enhances anticancer efficacy by modulating metabolic pathways and gut microbiome in hepatocellular carcinoma

    doi: 10.7150/ijbs.118753

    Figure Lengend Snippet: Combining sorafenib with spermine or sphingosine produces synergistic apoptotic effects in hepatocellular carcinoma through cell-cycle arrest. (A) A flow cytometry analysis was performed on SK-Hep-1 cells treated with sorafenib (Sora), spermine (Sper), and/or sphingosine (Sphi). (B) The percentage of cells in each cell-cycle phase was measured by flow cytometry, and the population of cells was plotted. SubG1, red; G1, blue; S, yellow; G2/M, green. (C) The percentage of cells in subG1 fraction was plotted. (D) Immunoblot analyses were performed on cells treated with sorafenib (Sora), spermine (Sper), and/or sphingosine (Sphi), either individually or in combination, using specific antibodies against cyclin A, cyclin E1, cyclin D1, p21 WAF1 , and β-actin. The relative band intensities were plotted. * p < 0.05; ** p < 0.01; *** p < 0.001 compared with control. (E) Immunofluorescence staining was performed in cells after single or combination treatment with sorafenib (Sora), spermine (Sper), and/or sphingosine (Sphi). Cleaved caspase-3 (green), α-tubulin (red), and DNA (DAPI, blue) are displayed. n>3000. The cell images were collected and evaluated with a confocal microscope FW3000 (Olympus; Tokyo, Japan). Scale bar, 20 μm. ( F ) The population of cleaved caspase-3 (green fluorescence)-positive cells was quantified. n>3000. * p < 0.05; ** p < 0.01; *** p < 0.001. Data are presented as the mean ± SD. ( G-H ) SK-Hep-1 cells were treated with sorafenib with and without spermine ( G ), or sorafenib with and without sphingosine ( H ) for 48 hours. Then the relative caspase-3 activity was measured with Ac-DEVD-AMC substrate and plotted. * p < 0.05; ** p < 0.01; *** p < 0.001 compared with control. Data are presented as the mean ± SD.

    Article Snippet: Cell lysates (30 μg) were treated with 200 nM Ac-DEVD-AMC (Med Chem Express; Princeton, NJ, USA) in reaction buffer (2 mM DTT, 20 mM HEPES [pH 7.5], and 10% glycerol) at 37 °C, in accordance with a previous study .

    Techniques: Flow Cytometry, Western Blot, Control, Immunofluorescence, Staining, Microscopy, Fluorescence, Activity Assay