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lskl (MedChemExpress)

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MedChemExpress lskl
Lskl, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hy-p0299a/LSKL%2C+Inhibitor+of+Thrombospondin/pm41412276-61-0-6
Average 94 stars, based on 4 article reviews

lskl - by Bioz Stars, 2026-10

94/100 stars

Images

Cell Culture:

Article Title: Macrophage Mtdh deficiency discordantly regulated tumor growth and metastasis through increased thrombospondin-1 production
Article Snippet: Peritoneal macrophages (PMs) were isolated from thioglycollate (211716, BD Biosciences)-elicited mice and purified by adherence to plastic culture dishes, as described previously. .. The adherent macrophages were cultured with or without LSKL peptide (an inhibitor of TSP-1-mediated activation of TGF-β1, HY-P0299A, MedChemExpress) or pifithrin-β (PFT-β, HY-16702A, MedChemExpress) for 48 hours. ..

Activation Assay:

Image Search Results

Blockade of TSP-1 with a TSP-1/TGF-β1 inhibitor suppresses fibrosis , subcutaneous tumor growth , and FMT induced by macrophages with Mtdh loss. ( A ) Tumor growth curves of subcutaneous MC-38 tumors from Mac- Mtdh KO or Mtdh fl/fl mice treated with or without LSKL (30 mg/kg, intraperitoneally once daily). Statistical significance was calculated by the two-way ANOVA. Phosphate buffer saline (PBS) was used as the solvent to dissolve LSKL. ( B ) Representative tumor images from the four experimental groups (n=5 mice per group). ( C ) Quantification of tumor weight in subcutaneous MC-38 tumors from the four groups. Statistical significance was calculated by one-way ANOVA. ( D ) Representative confocal images showing α-SMA expression in subcutaneous MC-38 tumors from the four groups. Scale bar: 40 µm. ( E ) Quantification of α-SMA-positive cells per field in subcutaneous MC-38 tumors. Statistical significance was calculated by one-way ANOVA. ( F ) Representative images of Transwell migration assays for NIH3T3 fibroblasts treated with LSKL (2.25 µg/mL, 48 hours) and supernatants from Mtdh fl/fl or Mac- Mtdh KO cocultures. Scale bar: 50 µm. ( G ) Quantification of migrated NIH3T3 fibroblasts shown in panel ( F ). Statistical significance was calculated by one-way ANOVA. ( H ) Representative western blot images and ( I ) quantitative analysis of α-SMA and fibronectin protein levels in NIH3T3 fibroblasts treated with LSKL (2.25 μg/mL, 48 hours) and supernatants from Mtdh fl/fl or Mac- Mtdh KO cocultures. Protein levels were normalized to GAPDH. Statistical significance was calculated by one-way ANOVA. Data are presented as the mean±SD from at least three independent experiments. *p<0.05, **p<0.01. TGF-β1, transforming growth factor β1. ANOVA, analysis of variance; FMT, fibroblast-to-myofibroblast transition; TGF-β1, transforming growth factor-β1; TSP-1, thrombospondin-1.

Journal: Journal for Immunotherapy of Cancer

Article Title: Macrophage Mtdh deficiency discordantly regulated tumor growth and metastasis through increased thrombospondin-1 production

doi: 10.1136/jitc-2025-013655

Figure Lengend Snippet: Blockade of TSP-1 with a TSP-1/TGF-β1 inhibitor suppresses fibrosis , subcutaneous tumor growth , and FMT induced by macrophages with Mtdh loss. ( A ) Tumor growth curves of subcutaneous MC-38 tumors from Mac- Mtdh KO or Mtdh fl/fl mice treated with or without LSKL (30 mg/kg, intraperitoneally once daily). Statistical significance was calculated by the two-way ANOVA. Phosphate buffer saline (PBS) was used as the solvent to dissolve LSKL. ( B ) Representative tumor images from the four experimental groups (n=5 mice per group). ( C ) Quantification of tumor weight in subcutaneous MC-38 tumors from the four groups. Statistical significance was calculated by one-way ANOVA. ( D ) Representative confocal images showing α-SMA expression in subcutaneous MC-38 tumors from the four groups. Scale bar: 40 µm. ( E ) Quantification of α-SMA-positive cells per field in subcutaneous MC-38 tumors. Statistical significance was calculated by one-way ANOVA. ( F ) Representative images of Transwell migration assays for NIH3T3 fibroblasts treated with LSKL (2.25 µg/mL, 48 hours) and supernatants from Mtdh fl/fl or Mac- Mtdh KO cocultures. Scale bar: 50 µm. ( G ) Quantification of migrated NIH3T3 fibroblasts shown in panel ( F ). Statistical significance was calculated by one-way ANOVA. ( H ) Representative western blot images and ( I ) quantitative analysis of α-SMA and fibronectin protein levels in NIH3T3 fibroblasts treated with LSKL (2.25 μg/mL, 48 hours) and supernatants from Mtdh fl/fl or Mac- Mtdh KO cocultures. Protein levels were normalized to GAPDH. Statistical significance was calculated by one-way ANOVA. Data are presented as the mean±SD from at least three independent experiments. *p<0.05, **p<0.01. TGF-β1, transforming growth factor β1. ANOVA, analysis of variance; FMT, fibroblast-to-myofibroblast transition; TGF-β1, transforming growth factor-β1; TSP-1, thrombospondin-1.

Article Snippet: The adherent macrophages were cultured with or without LSKL peptide (an inhibitor of TSP-1-mediated activation of TGF-β1, HY-P0299A, MedChemExpress) or pifithrin-β (PFT-β, HY-16702A, MedChemExpress) for 48 hours.

Techniques: Saline, Solvent, Expressing, Migration, Western Blot