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ha peptide  (MedChemExpress)


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    Structured Review

    MedChemExpress ha peptide
    Ha Peptide, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hy-p0239a/HA+Peptide/pmc10628743-247-32-34
    Average 94 stars, based on 10 article reviews
    ha peptide - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    other:

    Article Title: Cysteine allostery and autoinhibition govern human STING oligomer functionality
    Article Snippet: IRdye 800CW Azide Infrared Dye , , LI-Cor Biosciences , P/N: 929-60000.

    Immunoprecipitation:

    Article Title: Essential role of NONO-HOXA1-Wnt axis in cardiomyocyte differentiation.
    Article Snippet: .. Chromatin was first immunoprecipitated with 12 μg of anti-HA antibody and competitively eluted under native conditions using HA peptide (1 mg/mL) (MCE, HY-P0239). ..

    Article Title: The FBXW7‐RPAP2 Axis Controls the Growth of Hepatocellular Carcinoma Cells and Determines the Fate of Liver Cell Differentiation
    Article Snippet: .. Immunoprecipitated HA‐p38α and HA‐GSK3β were eluted from beads by incubating with 100 μL (1 mg mL −1 ) HA peptide (HY‐P0239, MCE). ..

    Incubation:

    Article Title: Exploring the role of SWI/SNF complex subunit BAF60c in lipid metabolism and inflammation in fish
    Article Snippet: The co-IP experiment was carried out following the protocol as previously described., Briefly, HEK293T cells transfected with BAF60c-HA and GRP78-FLAG plasmids were harvested at 24 h. Then, cells were lysed with Cell lysis buffer for Western and IP (Beyotime, China) at 4°C for 20 min, and the lysate was collected by centrifugation to get the supernatant. .. The supernatant was incubated with Pierce ANTI-HA agarose (Thermo Fisher Scientific, USA), ANTI-FLAG M2 Affinity Gel (Sigma, USA), and IgG-beads (Sigma, USA) at 4°C for 4 h. After washing, we used a HA peptide (MedChem Express, USA) or Flag peptide (MedChem Express) to detach the proteins from agarose beads for further analysis. ..

    Article Title: Tip60-mediated Rheb acetylation links palmitic acid with mTORC1 activation and insulin resistance.
    Article Snippet: .. For Co-IP, HEK293T cells were lysed with Cell Lysis Buffer for western Blotting and IP (Beyotime Biotechnology) for 20min after transfection with the indicated plasmids for 48 h. Then, the lysates were incubated with ANTI-Flag M2 Affinity Gel (SigmaAldrich) or Pierce anti-HA agarose (Thermo Fisher Scientific) at 4°C for 8–12 h. After washing with the lysis buffer and TBST five times, the binding components were eluted using the Flag peptide (MedChem Express) or HA peptide (MedChem Express) and analyzed by immunoblotting. ..

    Lysis:

    Article Title: Tip60-mediated Rheb acetylation links palmitic acid with mTORC1 activation and insulin resistance.
    Article Snippet: .. For Co-IP, HEK293T cells were lysed with Cell Lysis Buffer for western Blotting and IP (Beyotime Biotechnology) for 20min after transfection with the indicated plasmids for 48 h. Then, the lysates were incubated with ANTI-Flag M2 Affinity Gel (SigmaAldrich) or Pierce anti-HA agarose (Thermo Fisher Scientific) at 4°C for 8–12 h. After washing with the lysis buffer and TBST five times, the binding components were eluted using the Flag peptide (MedChem Express) or HA peptide (MedChem Express) and analyzed by immunoblotting. ..

    Western Blot:

    Article Title: Tip60-mediated Rheb acetylation links palmitic acid with mTORC1 activation and insulin resistance.
    Article Snippet: .. For Co-IP, HEK293T cells were lysed with Cell Lysis Buffer for western Blotting and IP (Beyotime Biotechnology) for 20min after transfection with the indicated plasmids for 48 h. Then, the lysates were incubated with ANTI-Flag M2 Affinity Gel (SigmaAldrich) or Pierce anti-HA agarose (Thermo Fisher Scientific) at 4°C for 8–12 h. After washing with the lysis buffer and TBST five times, the binding components were eluted using the Flag peptide (MedChem Express) or HA peptide (MedChem Express) and analyzed by immunoblotting. ..

    Transfection:

    Article Title: Tip60-mediated Rheb acetylation links palmitic acid with mTORC1 activation and insulin resistance.
    Article Snippet: .. For Co-IP, HEK293T cells were lysed with Cell Lysis Buffer for western Blotting and IP (Beyotime Biotechnology) for 20min after transfection with the indicated plasmids for 48 h. Then, the lysates were incubated with ANTI-Flag M2 Affinity Gel (SigmaAldrich) or Pierce anti-HA agarose (Thermo Fisher Scientific) at 4°C for 8–12 h. After washing with the lysis buffer and TBST five times, the binding components were eluted using the Flag peptide (MedChem Express) or HA peptide (MedChem Express) and analyzed by immunoblotting. ..

    Binding Assay:

    Article Title: Tip60-mediated Rheb acetylation links palmitic acid with mTORC1 activation and insulin resistance.
    Article Snippet: .. For Co-IP, HEK293T cells were lysed with Cell Lysis Buffer for western Blotting and IP (Beyotime Biotechnology) for 20min after transfection with the indicated plasmids for 48 h. Then, the lysates were incubated with ANTI-Flag M2 Affinity Gel (SigmaAldrich) or Pierce anti-HA agarose (Thermo Fisher Scientific) at 4°C for 8–12 h. After washing with the lysis buffer and TBST five times, the binding components were eluted using the Flag peptide (MedChem Express) or HA peptide (MedChem Express) and analyzed by immunoblotting. ..



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    MedChemExpress ha peptide
    Ha Peptide, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hy-p0239a/HA+Peptide/pm41568710-118-21-23
    Average 94 stars, based on 1 article reviews
    ha peptide - by Bioz Stars, 2026-10
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