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cdk2 inhibitor (MedChemExpress)

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MedChemExpress cdk2 inhibitor

Fluorescence images of human IVM-MⅡ oocytes by laser confocal microscopy and statistical results of repetitive laser confocal assay experiments on the effect of 10 –6 M NADH. Scale bar, 20 μm. (A, B) Effect of NADH on <t>CDK2</t> gene in IVM-MⅡ oocytes. Sample size: Control group: n = 17; NADH group: n = 18. (C, D) Effect of NADH on GAS6 gene in IVM-MⅡ oocytes. Sample size: Control group: n = 17; NADH group: n = 15. (E, F) Effect of NADH on C14orf36 gene in IVM-MⅡ oocytes. Sample size: Control group: n = 14; NADH group: n = 16. (G, H) Effect of NADH on DCM1 gene in IVM-MⅡ oocytes. Sample size: Control group: n = 15; NADH group: n = 18. (I, J) Effect of NADH on FIGN gene in IVM-MⅡ oocytes. Sample size: Control group: n = 15; NADH group: n = 24. (K, L) Effect of NADH on FAM9B gene in IVM-MⅡ oocytes. Sample size: Control group: n = 17; NADH group: n = 17. **P < 0.01, ***P < 0.001.

Cdk2 Inhibitor, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hy-p0235/CDK2/pmc12440754-92-16-18
Average 94 stars, based on 22 article reviews

cdk2 inhibitor - by Bioz Stars, 2026-10

94/100 stars

Images

1) Product Images from "NADH supplementation improves human oocyte maturation and developmental competence of resulting embryos in controlled ovarian hyperstimulation cycles: a pilot study implicating the CDK2/GAS6 signaling pathway"

Journal: Frontiers in Endocrinology

doi: 10.3389/fendo.2025.1627679

Figure Legend Snippet: Fluorescence images of human IVM-MⅡ oocytes by laser confocal microscopy and statistical results of repetitive laser confocal assay experiments on the effect of 10 –6 M NADH. Scale bar, 20 μm. (A, B) Effect of NADH on CDK2 gene in IVM-MⅡ oocytes. Sample size: Control group: n = 17; NADH group: n = 18. (C, D) Effect of NADH on GAS6 gene in IVM-MⅡ oocytes. Sample size: Control group: n = 17; NADH group: n = 15. (E, F) Effect of NADH on C14orf36 gene in IVM-MⅡ oocytes. Sample size: Control group: n = 14; NADH group: n = 16. (G, H) Effect of NADH on DCM1 gene in IVM-MⅡ oocytes. Sample size: Control group: n = 15; NADH group: n = 18. (I, J) Effect of NADH on FIGN gene in IVM-MⅡ oocytes. Sample size: Control group: n = 15; NADH group: n = 24. (K, L) Effect of NADH on FAM9B gene in IVM-MⅡ oocytes. Sample size: Control group: n = 17; NADH group: n = 17. **P < 0.01, ***P < 0.001.

Techniques Used: Fluorescence, Confocal Microscopy, Confocal Assay, Control

Working model for the impact of NADH on the quality of COH immature oocytes. Presented new findings on IVM culture of human immature oocytes during the COH cycle: The addition of 10 –6 M NADH in IVM medium can effectively improve the developmental potential of IVM-MⅡ oocytes. The mechanism is that the application of NADH significantly increases the levels of ATP, MMP and GSH in oocytes, and realizes the improvement of mitochondrial function and antioxidant capacity of IVM- MⅡ oocytes. NADH up-regulates CDK2 and GAS6 genes, protected the cell cycle progression and promoted cytoplasmic maturation and facilitates embryonic development respectively.

Figure Legend Snippet: Working model for the impact of NADH on the quality of COH immature oocytes. Presented new findings on IVM culture of human immature oocytes during the COH cycle: The addition of 10 –6 M NADH in IVM medium can effectively improve the developmental potential of IVM-MⅡ oocytes. The mechanism is that the application of NADH significantly increases the levels of ATP, MMP and GSH in oocytes, and realizes the improvement of mitochondrial function and antioxidant capacity of IVM- MⅡ oocytes. NADH up-regulates CDK2 and GAS6 genes, protected the cell cycle progression and promoted cytoplasmic maturation and facilitates embryonic development respectively.

Techniques Used:

Bioprocessing:

Article Title: MS 275 Inhibits Neuroblastoma Cell Growth by Mediating H 3 K 27ac/ PROX 1 Axis In Silico and In Vitro
Article Snippet: Briefly, total protein was extracted from the cells using RIPA lysis buffer (Beyotime), and the cell lysates were subjected to western blot with specific antibodies. .. The monoclonal antibodies (mAbs) against HDAC1, HDAC3, Phospho‐CDK2 (Tyr15), CDK2, Phospho‐RB (Ser807), and CDK4 were from MedChemExpress. .. The monoclonal antibodies (mAbs) against α‐tubulin and β‐actin, and the polyclonal antibodies for Cyclin D1, Cyclin E1, BAX, LC3B, P62, and PROX1 were purchased from Proteintech.

other:

Article Title: NADH supplementation improves human oocyte maturation and developmental competence of resulting embryos in controlled ovarian hyperstimulation cycles: a pilot study implicating the CDK2/GAS6 signaling pathway
Article Snippet: Immature oocytes obtained through COH cycle were randomly allocated into IVM culture medium with or without CDK2 inhibitor (MedChemExpress, NJ, USA), NADH, and NADH + CDK2 inhibitor.

Biomarker Discovery:

Article Title: Differential splice isoforms of mouse CDK2 play functionally redundant roles during mitotic and meiotic division.
Article Snippet: .. For the validation of the antibodies against CDK2L, we have immunoprecipitated CDK2 (both CDK2S and CDK2L) using 4 μL of mouse monoclonal Cdk2 antibody (Santa Cruz, sc-6248) and 10 μL Protein A agarose slurry (MedChemExpress, HY-K0213) from mouse testis extract, followed by western blot with rabbit anti CDK2 antibodies [homemade; (Berthet et al., 2003)] at 1:5000 dilution (Figure 2, lanes 1-3) or rabbit antibodies against CDK2L (homemade) at 1:1000 dilution, followed by a secondary anti-rabbit antibody at 1:5000 dilution. ..

Article Title: Differential splice isoforms of mouse CDK2 play functionally redundant roles during mitotic and meiotic division
Article Snippet: .. For the validation of the antibodies against CDK2L, we immunoprecipitated CDK2 (both CDK2S and CDK2L) using 4 μl of mouse monoclonal anti-Cdk2 antibody (Santa Cruz Biotechnology, sc-6248) and 10 μl Protein A agarose slurry (MedChemExpress, HY-K0213) from mouse testis extract, followed by western blotting with rabbit anti-CDK2 antibodies ( ) at 1:5000 ( , lanes 1-3) or rabbit antibodies against CDK2L ( ) at 1:1000, followed by a secondary anti-rabbit IgG antibody (goat anti-rabbit IgG conjugated to HRP, Invitrogen, 31462) at 1:5000. ..

Immunoprecipitation:

Western Blot:

Control:

Article Title: Butein suppresses pancreatic cancer progression by downregulating CDK2 and disrupting CDK2/CyclinA2 interaction.
Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

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Design of the CDK2S and CDK2L mice. Top: Deletion of exon 6 leads to mice that can only express CDK2S (Exon 6 knock-out; Cdk2 SHORT/SHORT ). Bottom: Fusion of exons 5-7 leads to the continuous expression of CDK2L and the absence of CDK2S (Exon 6 knock-in; Cdk2 LONG/LONG ).

Journal: Journal of Cell Science

Article Title: Differential splice isoforms of mouse CDK2 play functionally redundant roles during mitotic and meiotic division

doi: 10.1242/jcs.264291

Figure Lengend Snippet: Design of the CDK2S and CDK2L mice. Top: Deletion of exon 6 leads to mice that can only express CDK2S (Exon 6 knock-out; Cdk2 SHORT/SHORT ). Bottom: Fusion of exons 5-7 leads to the continuous expression of CDK2L and the absence of CDK2S (Exon 6 knock-in; Cdk2 LONG/LONG ).

Article Snippet: For the validation of the antibodies against CDK2L, we immunoprecipitated CDK2 (both CDK2S and CDK2L) using 4 μl of mouse monoclonal anti-Cdk2 antibody (Santa Cruz Biotechnology, sc-6248) and 10 μl Protein A agarose slurry (MedChemExpress, HY-K0213) from mouse testis extract, followed by western blotting with rabbit anti-CDK2 antibodies ( ) at 1:5000 ( , lanes 1-3) or rabbit antibodies against CDK2L ( ) at 1:1000, followed by a secondary anti-rabbit IgG antibody (goat anti-rabbit IgG conjugated to HRP, Invitrogen, 31462) at 1:5000.

Techniques: Knock-Out, Expressing, Knock-In

Protein expression of CDK2S and CDK2L in tissue extracts. (A) Protein extracts from testis, spleen, thymus and brain were analysed by western blotting using pan-CDK2 antibodies. Note that p39 corresponds to CDK2L and p33 to CDK2S, respectively. (B) Testis protein extracts from wild-type, Cdk2 SHORT/SHORT (CDK2S), and Cdk2 LONG/LONG (CDK2L) mice were analysed for CDK2 expression. Note that HSP90 serves as a loading control in A and B. (C) To determine the specificity of the antibodies against CDK2L, we immunoprecipitated CDK2 followed by western blotting with either pan-CDK2 antibodies (lanes 1-3) or antibodies against CDK2L (lanes 4-6). The antibodies against CDK2L only recognized the 39 kDa isoform of CDK2 (CDK2L) and not the 33 kDa isoform (CDK2S).

Journal: Journal of Cell Science

Article Title: Differential splice isoforms of mouse CDK2 play functionally redundant roles during mitotic and meiotic division

doi: 10.1242/jcs.264291

Figure Lengend Snippet: Protein expression of CDK2S and CDK2L in tissue extracts. (A) Protein extracts from testis, spleen, thymus and brain were analysed by western blotting using pan-CDK2 antibodies. Note that p39 corresponds to CDK2L and p33 to CDK2S, respectively. (B) Testis protein extracts from wild-type, Cdk2 SHORT/SHORT (CDK2S), and Cdk2 LONG/LONG (CDK2L) mice were analysed for CDK2 expression. Note that HSP90 serves as a loading control in A and B. (C) To determine the specificity of the antibodies against CDK2L, we immunoprecipitated CDK2 followed by western blotting with either pan-CDK2 antibodies (lanes 1-3) or antibodies against CDK2L (lanes 4-6). The antibodies against CDK2L only recognized the 39 kDa isoform of CDK2 (CDK2L) and not the 33 kDa isoform (CDK2S).

Techniques: Expressing, Western Blot, Control, Immunoprecipitation

CDK2S and CDK2L mice are fertile. (A) P30 body weight, testis weight, and testis weight as a percentage of bodyweight for wild type (black circles, n =15), Cdk2 SHORT/SHORT (CDK2S; orange squares, n =5) and Cdk2 LONG/LONG (CDK2L, blue triangles, n =3). * P <0.05 and ** P <0.01 (one-way ANOVA with Tukey's multiple comparison post-hoc test). ns. not significant. Bars and error bars represent mean and s.d., respectively. (B) Cumulative pups born over time for crosses between Cdk2 +/SHORT (red; N =9 breeding pairs), Cdk2 SHORT/SHORT (orange; N =4 breeding pairs), Cdk2 +/LONG (green; N =8 breeding pairs) or Cdk2 LONG/LONG (blue; N =8 breeding pairs). Data are presented as the mean number of cumulative pups born to breeding pairs of each condition (±s.d.). One-way ANOVA analysis indicated that there is no statistical significance between heterozygous groups. Day 0 indicates the start of breeding, which was initiated when both male and female mice of a single breeding pair were 8-10 weeks of age. (C-F) Gender balance of the pups from the breedings shown in B. (G) Percentage of wild-type ( Cdk2 +/+ ), heterozygote ( Cdk2 +/SHORT ) and homozygote ( Cdk2 SHORT/SHORT ) pups from heterozygote intercrosses. (H) Percentage of wild-type ( Cdk2 +/+ ), heterozygote ( Cdk2 +/LONG ) and homozygote ( Cdk2 LONG/LONG ) pups from heterozygote intercrosses. Note that the expected 25%, 50%, 25% Mendelian ratios for genotype, and 50% for male and female ratios were observed, tested by χ 2 goodness of fit test. Percentages in graphs in C-H were calculated based on the number of pups born for given traits (e.g. gender or genotype) in a breeding pair. Boxes represent the interquartile range of the percentage of pups per breeding pair ( n =8 for heterozygous and n =4 for homozygous). The median is indicated. The whiskers show values extending to 1.5× interquartle range from the quartile 1 and quartile 3 boundaries.

Journal: Journal of Cell Science

Article Title: Differential splice isoforms of mouse CDK2 play functionally redundant roles during mitotic and meiotic division

doi: 10.1242/jcs.264291

Figure Lengend Snippet: CDK2S and CDK2L mice are fertile. (A) P30 body weight, testis weight, and testis weight as a percentage of bodyweight for wild type (black circles, n =15), Cdk2 SHORT/SHORT (CDK2S; orange squares, n =5) and Cdk2 LONG/LONG (CDK2L, blue triangles, n =3). * P <0.05 and ** P <0.01 (one-way ANOVA with Tukey's multiple comparison post-hoc test). ns. not significant. Bars and error bars represent mean and s.d., respectively. (B) Cumulative pups born over time for crosses between Cdk2 +/SHORT (red; N =9 breeding pairs), Cdk2 SHORT/SHORT (orange; N =4 breeding pairs), Cdk2 +/LONG (green; N =8 breeding pairs) or Cdk2 LONG/LONG (blue; N =8 breeding pairs). Data are presented as the mean number of cumulative pups born to breeding pairs of each condition (±s.d.). One-way ANOVA analysis indicated that there is no statistical significance between heterozygous groups. Day 0 indicates the start of breeding, which was initiated when both male and female mice of a single breeding pair were 8-10 weeks of age. (C-F) Gender balance of the pups from the breedings shown in B. (G) Percentage of wild-type ( Cdk2 +/+ ), heterozygote ( Cdk2 +/SHORT ) and homozygote ( Cdk2 SHORT/SHORT ) pups from heterozygote intercrosses. (H) Percentage of wild-type ( Cdk2 +/+ ), heterozygote ( Cdk2 +/LONG ) and homozygote ( Cdk2 LONG/LONG ) pups from heterozygote intercrosses. Note that the expected 25%, 50%, 25% Mendelian ratios for genotype, and 50% for male and female ratios were observed, tested by χ 2 goodness of fit test. Percentages in graphs in C-H were calculated based on the number of pups born for given traits (e.g. gender or genotype) in a breeding pair. Boxes represent the interquartile range of the percentage of pups per breeding pair ( n =8 for heterozygous and n =4 for homozygous). The median is indicated. The whiskers show values extending to 1.5× interquartle range from the quartile 1 and quartile 3 boundaries.

Techniques: Comparison

Morphology of testis and ovary. (A) H&E-stained P30 (30 days postpartum) testis sections from wild type, Cdk2 SHORT/SHORT (CDK2S) and Cdk2 LONG/LONG (CDK2L). Boxed areas are shown at higher magnification beneath. Arrows indicate pre-leptotene (PL; orange), pachytene (P; green), spermatocytes, and round spermatids (RS; yellow). Scale bars: 60 µm (top row); 15 µm (bottom row). (B) H&E-stained P56 ovary sections from wild type, Cdk2 SHORT/SHORT (CDK2S) and Cdk2 LONG/LONG (CDK2L). Boxed areas are shown at higher magnification beneath to illustrate follicles. Scale bars: 60 µm (top row); 15 µm (bottom row).

Journal: Journal of Cell Science

Article Title: Differential splice isoforms of mouse CDK2 play functionally redundant roles during mitotic and meiotic division

doi: 10.1242/jcs.264291

Figure Lengend Snippet: Morphology of testis and ovary. (A) H&E-stained P30 (30 days postpartum) testis sections from wild type, Cdk2 SHORT/SHORT (CDK2S) and Cdk2 LONG/LONG (CDK2L). Boxed areas are shown at higher magnification beneath. Arrows indicate pre-leptotene (PL; orange), pachytene (P; green), spermatocytes, and round spermatids (RS; yellow). Scale bars: 60 µm (top row); 15 µm (bottom row). (B) H&E-stained P56 ovary sections from wild type, Cdk2 SHORT/SHORT (CDK2S) and Cdk2 LONG/LONG (CDK2L). Boxed areas are shown at higher magnification beneath to illustrate follicles. Scale bars: 60 µm (top row); 15 µm (bottom row).

Techniques: Staining

The synaptonemal complex forms successfully in CDK2S and CDK2L testis. (A-R) Chromosome spreads were prepared from adult testis and stained with antibodies against SYCP1 (green) and SYCP3 (magenta). Various stages of meiotic prophase I are depicted for wild type (A-F), Cdk2 SHORT/SHORT (G-L; CDK2S) and Cdk2 LONG/LONG (M-R; CDK2L). Spermatocytes from all three genotypes progress comparably into mid-pachytene (D,J,P), late-pachytene (E,K,Q) and diplotene (F,L,R). The sex chromosomes are labelled by a blue ‘XY’. At least three biological replicates were analysed for each condition. Scale bars: 5 µm.

Journal: Journal of Cell Science

Article Title: Differential splice isoforms of mouse CDK2 play functionally redundant roles during mitotic and meiotic division

doi: 10.1242/jcs.264291

Figure Lengend Snippet: The synaptonemal complex forms successfully in CDK2S and CDK2L testis. (A-R) Chromosome spreads were prepared from adult testis and stained with antibodies against SYCP1 (green) and SYCP3 (magenta). Various stages of meiotic prophase I are depicted for wild type (A-F), Cdk2 SHORT/SHORT (G-L; CDK2S) and Cdk2 LONG/LONG (M-R; CDK2L). Spermatocytes from all three genotypes progress comparably into mid-pachytene (D,J,P), late-pachytene (E,K,Q) and diplotene (F,L,R). The sex chromosomes are labelled by a blue ‘XY’. At least three biological replicates were analysed for each condition. Scale bars: 5 µm.

Techniques: Staining

The repair of recombination intermediates. Chromosome spreads were prepared from adult testis and stained with antibodies against γH2AX (green, DNA damage) and SYCP3 (red). Various stages of meiotic prophase I are depicted for wild type (A-F), Cdk2 SHORT/SHORT (G-L; CDK2S) and Cdk2 LONG/LONG (M-R; CDK2L). DNA intermediates are repaired at the correct time with a cloud-like staining in leptotene (A,G,M) and zygotene (B,H,N). Thereafter, γH2AX staining is mostly found along the axis (C,I,O) and retreats over time starting at mid-pachytene (D,J,P,E,K,Q,F,L,R). The sex chromosomes are labelled by a blue ‘XY’. At least three biological replicates were analysed for each condition. Scale bars: 5 µm. (S-Z) Statistics for overall H2AX fluorescence intensity for each stage (S-X) and overlap of H2AX and SYCP3 fluorescence intensity for early-pachytene (Y) and mid-pachytene (Z). * P <0.05, ** P <0.01. Error bars show mean and s.e.m. Details of the statistical analysis are described in the Materials and Methods. ns, not significant. MFI, mean fluorescence intensity

Journal: Journal of Cell Science

Article Title: Differential splice isoforms of mouse CDK2 play functionally redundant roles during mitotic and meiotic division

doi: 10.1242/jcs.264291

Figure Lengend Snippet: The repair of recombination intermediates. Chromosome spreads were prepared from adult testis and stained with antibodies against γH2AX (green, DNA damage) and SYCP3 (red). Various stages of meiotic prophase I are depicted for wild type (A-F), Cdk2 SHORT/SHORT (G-L; CDK2S) and Cdk2 LONG/LONG (M-R; CDK2L). DNA intermediates are repaired at the correct time with a cloud-like staining in leptotene (A,G,M) and zygotene (B,H,N). Thereafter, γH2AX staining is mostly found along the axis (C,I,O) and retreats over time starting at mid-pachytene (D,J,P,E,K,Q,F,L,R). The sex chromosomes are labelled by a blue ‘XY’. At least three biological replicates were analysed for each condition. Scale bars: 5 µm. (S-Z) Statistics for overall H2AX fluorescence intensity for each stage (S-X) and overlap of H2AX and SYCP3 fluorescence intensity for early-pachytene (Y) and mid-pachytene (Z). * P <0.05, ** P <0.01. Error bars show mean and s.e.m. Details of the statistical analysis are described in the Materials and Methods. ns, not significant. MFI, mean fluorescence intensity

Techniques: Staining, Fluorescence

Speedy A can interact with both CDK2S and CDK2L. (A-R) Chromosome spreads from adult testis stained with antibodies against Speedy A (red), pan-CDK2 (green) and SYCP3 (blue). Various stages of meiotic prophase I are depicted for wild type (A-F), Cdk2 SHORT/SHORT (G-L; CDK2S) and Cdk2 LONG/LONG (M-R; CDK2L). Speedy A localizes to the telomeres in all genotypes, i.e. wild type (both CDK2S and CDK2L), Cdk2 SHORT/SHORT or Cdk2 LONG/LONG . The sex chromosomes are labelled by a grey ‘XY’. Boxed areas are shown at higher magnification in insets I-XII. Yellow arrows indicate telomeres, and magenta arrows interstitial sites. At least three biological replicates were analysed for each condition. Scale bars: 5 µm (main panels); 1.25 µm (insets, I-XII).

Journal: Journal of Cell Science

Article Title: Differential splice isoforms of mouse CDK2 play functionally redundant roles during mitotic and meiotic division

doi: 10.1242/jcs.264291

Figure Lengend Snippet: Speedy A can interact with both CDK2S and CDK2L. (A-R) Chromosome spreads from adult testis stained with antibodies against Speedy A (red), pan-CDK2 (green) and SYCP3 (blue). Various stages of meiotic prophase I are depicted for wild type (A-F), Cdk2 SHORT/SHORT (G-L; CDK2S) and Cdk2 LONG/LONG (M-R; CDK2L). Speedy A localizes to the telomeres in all genotypes, i.e. wild type (both CDK2S and CDK2L), Cdk2 SHORT/SHORT or Cdk2 LONG/LONG . The sex chromosomes are labelled by a grey ‘XY’. Boxed areas are shown at higher magnification in insets I-XII. Yellow arrows indicate telomeres, and magenta arrows interstitial sites. At least three biological replicates were analysed for each condition. Scale bars: 5 µm (main panels); 1.25 µm (insets, I-XII).

Techniques: Staining

CDK2L can localize to interstitial sites in the absence of CDK2S. (A-R) Chromosome spreads were prepared from adult testis for wild type (A-F), Cdk2 SHORT/SHORT (G-L; CDK2S) and Cdk2 LONG/LONG (M-R; CDK2L) and stained with antibodies against CDK2L (red), pan-CDK2 (green; detecting both CDK2S and CDK2L) and SYCP3 (blue). CDK2S can bind to both telomeres and interstitial sites (I-XII; green). In wild type, CDK2L binds to telomeres but not interstitial sites (I-IV; red); in Cdk2 SHORT/SHORT CDK2L cannot be detected (V-VIII); and in Cdk2 LONG/LONG CDK2L binds prominently to telomeres and weakly to interstitial sites (IX-XII; red). The sex chromosomes are labelled by a grey ‘XY’. Boxed areas are shown at higher magnification in insets I-XII. Yellow arrows indicate telomeres, and magenta arrows interstitial sites. At least three biological replicates were analysed for each condition. Scale bars: 5 µm (main panels); 1.25 µm (insets, I-XII).

Journal: Journal of Cell Science

Article Title: Differential splice isoforms of mouse CDK2 play functionally redundant roles during mitotic and meiotic division

doi: 10.1242/jcs.264291

Figure Lengend Snippet: CDK2L can localize to interstitial sites in the absence of CDK2S. (A-R) Chromosome spreads were prepared from adult testis for wild type (A-F), Cdk2 SHORT/SHORT (G-L; CDK2S) and Cdk2 LONG/LONG (M-R; CDK2L) and stained with antibodies against CDK2L (red), pan-CDK2 (green; detecting both CDK2S and CDK2L) and SYCP3 (blue). CDK2S can bind to both telomeres and interstitial sites (I-XII; green). In wild type, CDK2L binds to telomeres but not interstitial sites (I-IV; red); in Cdk2 SHORT/SHORT CDK2L cannot be detected (V-VIII); and in Cdk2 LONG/LONG CDK2L binds prominently to telomeres and weakly to interstitial sites (IX-XII; red). The sex chromosomes are labelled by a grey ‘XY’. Boxed areas are shown at higher magnification in insets I-XII. Yellow arrows indicate telomeres, and magenta arrows interstitial sites. At least three biological replicates were analysed for each condition. Scale bars: 5 µm (main panels); 1.25 µm (insets, I-XII).

Techniques: Staining

(A and B) MDA-MB-231, MDA-MB-361, and HCC1954 cells were seeded into 96-well plates (1 ×10 4 cells/well). After 24 h, the cells were incubated with DCA (A) or dinaciclib (B) at the indicated concentrations for 48 h, and cell proliferation was assessed using a CCK-8 assay. (C) Synergistic anti-tumor effects of DCA plus dinaciclib in BC cells. (D) MCF-7, MDA-MB-231, MDA-MB-361, and HCC1954 cells were treated with alpelisib, and then cell proliferation was assessed using a CCK-8 assay. (E) IC 50 values of alpelisib in the 4 cell lines. (F) MCF-7 and HCC1954 cells were treated with alpelisib and FOXK2 shRNAs, and cell proliferation was assessed using a CCK-8 assay. (G) Synergistic anti-tumor effects of alpelisib plus dinaciclib or DCA.

Journal: Cancer letters

Article Title: FOXK2 amplification promotes breast cancer development and chemoresistance

doi: 10.1016/j.canlet.2024.217074

Figure Lengend Snippet: (A and B) MDA-MB-231, MDA-MB-361, and HCC1954 cells were seeded into 96-well plates (1 ×10 4 cells/well). After 24 h, the cells were incubated with DCA (A) or dinaciclib (B) at the indicated concentrations for 48 h, and cell proliferation was assessed using a CCK-8 assay. (C) Synergistic anti-tumor effects of DCA plus dinaciclib in BC cells. (D) MCF-7, MDA-MB-231, MDA-MB-361, and HCC1954 cells were treated with alpelisib, and then cell proliferation was assessed using a CCK-8 assay. (E) IC 50 values of alpelisib in the 4 cell lines. (F) MCF-7 and HCC1954 cells were treated with alpelisib and FOXK2 shRNAs, and cell proliferation was assessed using a CCK-8 assay. (G) Synergistic anti-tumor effects of alpelisib plus dinaciclib or DCA.

Article Snippet: The CDK2 inhibitors dinaciclib (HY-10492) and alpelisib (BYL-719, HY-15244) were from MedChemExpress.

Techniques: Incubation, CCK-8 Assay

(A) Treatment strategy of the BC mouse model. (B) Bioluminescent imaging photos of MCF7-FLuc cells on subcutaneous tumor growth in NSG mice from all treatment groups (alpelisib alone, dinaciclib alone, and the combination) and the control group. (C) The values of bioluminescent imaging of BC tumor-bearing mice were monitored during tumor growth. Total flux of tumor in control and treated mice adjusted to baseline pretreatment values, log10 transformed, and plotted as mean ± S.D.. (D) Kaplan-Meier survival plot for the groups of mice shown in B and C.

Journal: Cancer letters

Article Title: FOXK2 amplification promotes breast cancer development and chemoresistance

doi: 10.1016/j.canlet.2024.217074

Figure Lengend Snippet: (A) Treatment strategy of the BC mouse model. (B) Bioluminescent imaging photos of MCF7-FLuc cells on subcutaneous tumor growth in NSG mice from all treatment groups (alpelisib alone, dinaciclib alone, and the combination) and the control group. (C) The values of bioluminescent imaging of BC tumor-bearing mice were monitored during tumor growth. Total flux of tumor in control and treated mice adjusted to baseline pretreatment values, log10 transformed, and plotted as mean ± S.D.. (D) Kaplan-Meier survival plot for the groups of mice shown in B and C.

Article Snippet: The CDK2 inhibitors dinaciclib (HY-10492) and alpelisib (BYL-719, HY-15244) were from MedChemExpress.

Techniques: Imaging, Control, Transformation Assay

Review

cdk2 inhibitor (MedChemExpress)

Structured Review

Images

1) Product Images from "NADH supplementation improves human oocyte maturation and developmental competence of resulting embryos in controlled ovarian hyperstimulation cycles: a pilot study implicating the CDK2/GAS6 signaling pathway"

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Bioprocessing:

other:

Biomarker Discovery:

Immunoprecipitation:

Western Blot:

Control:

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