Review

sc 221947 melittin (MedChemExpress)

MedChemExpress is a verified supplier

MedChemExpress manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

MedChemExpress sc 221947 melittin
Sc 221947 Melittin, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hy-p0233a/Melittin/pmc12078643__41467_2025_59249_MOESM1_ESM-10-123-127
Average 94 stars, based on 12 article reviews

sc 221947 melittin - by Bioz Stars, 2026-10

94/100 stars

Images

Saline:

Article Title: Melittin inhibits tumor cell migration and enhances cisplatin sensitivity by suppressing IL-17 signaling pathway gene LCN2 in castration-resistant prostate cancer.
Article Snippet: Funding information National Natural Science Foundation of China, Grant/Award Number: 82072846 Abstract Background: Melittin is a small molecule polypeptide extracted from the abdominal cavity of bees, which is used to treat inflammatory diseases and relieve pain.. However, the antitumor effect of melittin and its mechanisms remain unclear, especially in castration‐resistant prostate cancer (CRPC).. Methods: Through CCK‐8 assay, colony formation assay, wound healing assay and Transwell migration assay, we explored the effect of melittin on CRPC cell lines.

Reverse Transcription:

Article Title: Intermittent fasting reduces alpha-synuclein pathology and functional decline in a mouse model of Parkinson’s disease
Article Snippet: Anti-Iba1, rabbit 1:1000 Fujifilm Wako Chemicals Cat# 019-19741 Anti-GFAP, chicken 1:2000 Abcam Cat# ab4674 Anti-Tubulin βIII, rabbit 1:5000 Covance Cat# PRB-435P Anti-phospho-aSyn, rabbit 1:1000 Abcam Cat# ab51253 Anti-aSyn, human specific, rat 1:800 Enzo Cat# ALX-804-258 Ant-aSyn, rodent specific, rabbit 1:1000 Cell Signaling Cat# 4179 Anti-TH, sheep 1:2000 Pel Freez Cat# P40101 Anti-PSD95, rabbit 1.1000 Synaptic Systems Cat# 160002 Anti-LC3, rabbit 1:1000 Cell Signaling Cat# 2775 Anti LAMP1, rabbit 1:1000 Cell Signaling Cat# 9091 Anti-MAP2, chicken 1:4000 Abcam Cat# ab5392 Anti-Synapsin, chicken 1:1000 Synaptic System Cat# 106006 Anti-MAP2, A488 conjugated, rabbit 1:500 Abcam Cat#: ab225316 Anti-53BP1, rabbit 1:1000 Thermo Fisher Cat#: PA5-54565 Anti-SQSTM1/p62, rabbit 1:10000 Abcam Cat#: ab109012 Anti-NLRP3, mouse 1:1000 AdipoGen Cat#: AG-20B-0014 Anti-Arginase-1, mouse 1:2000 BD Biosciences Cat#: 610708 Anti-CD206/MRC1, rabbit 1:300 Thermo Fisher Cat#: PA5-114370 Anti-TNF, rabbit 1:1000 Thermo Fisher Cat#: PA1-40281 HRP conjugated donkey anti-mouse IgG 1:5000 Jackson ImmunoResearch Cat# 715-035-150 HRP conjugated donkey anti-rabbit IgG 1:5000 Jackson ImmunoResearch Cat# 711-035-152 HRP conjugated goat anti-mouse IgG 1:5000 Bio-Rad Cat#: 1706516 Alexa 555 conjugated donkey anti-rabbit 1:2000 Invitrogen Cat# A31572 Alexa 647 conjugated donkey anti-chicken 1:2000 Jackson ImmunoResearch Cat# 703-605-155 Alexa 488 conjugated donkey anti-sheep 1:2000 Invitrogen Cat# A11015 Alexa 488 conjugated donkey anti-rabbit 1:2000 Invitrogen Cat# A21206 .. PhosSTOP phosphatase inhibitor Sigma-Aldrich Cat# 4906845001 Bromphenol blue Honeywell Fluka Cat# 32712 Mini-PROTEAN TGX Precast Gels 4-20% BioRad Cat# 4561094 Precision Plus Protein WesternC Protein Standards BioRad Cat# 1610376 WesternBright Chemiluminescence Substrate Sirius Biozym Scientific GmbH Cat# 541019 Fluoromount-G Southern Biotech Cat# 0100-01 LDH Cytotoxicity Detection kit Merck Cat# 4744926001 BHB Assay kit Sigma Cat# MAK041 RNeasy Mini Kit Qiagen Cat# 74104 QuantiTect Reverse Transcription kit Qiagen Cat# 205313 GoTaq®aPCR Master Mix Promega Cat# A6102 RNeasy Lipid Tissue Mini Kit Qiagen Cat#: 74804 NEBNext Ultra II Directional RNA Library Prep Kit New England Biolabs Cat#: E7760 CCL17 40 nM Medchem Express Cat# HY-P71891A IL-36RN 100 ng/ml MedChem Express Cat# HY-P77007 CDK2-IN-3 100 nM MedChem Express Cat# HY-112460 MJ33 40 μM Santa Cruz Cat# sc-221947 Melittin 2 μM MedChem Express Cat# HY-P0233 ..

other:

Article Title: Mutations in the essential outer membrane protein BamA contribute to Escherichia coli resistance to the antimicrobial peptide TAT-RasGAP 317-326
Article Snippet: MRL-494 was provided by Medchemexpress and melittin by Enzo Life Sciences.

Article Title: Cloxiquine exerts bactericidal activity against Enterococcus faecalis through disruption of proton motive force and induction of reactive oxygen species
Article Snippet: Antimicrobial agents, including Cloxiquine, vancomycin, and melittin, were purchased from MedChem-Express (Monmouth Junction, NJ, USA) and dissolved in dimethyl sulfoxide (DMSO) or double-distilled water for use.

Article Title: Imeglimin suppresses glucagon secretion and induces a loss of α cell identity
Article Snippet: Melittin , MedChemExpress , CatHY-P0233.

Image Search Results

BamA point mutations increasing resistance to TAT-RasGAP 317-326 do not cause broad-range resistance to AMPs and classical antibiotics but can influence the efficiency of BamA inhibitors. MICs of the AMP polymyxin B ( A ), the antibiotic gentamicin ( B ), the BAM complex inhibitors MRL-494 ( C ), and darobactin ( D ) on the indicated strains were measured in triplicate. Dashed lines correspond to the average MIC of the wild-type. Continuous lines represent the average MIC for the three replicates.

Journal: The Journal of Biological Chemistry

Article Title: Mutations in the essential outer membrane protein BamA contribute to Escherichia coli resistance to the antimicrobial peptide TAT-RasGAP 317-326

doi: 10.1016/j.jbc.2024.108018

Figure Lengend Snippet: BamA point mutations increasing resistance to TAT-RasGAP 317-326 do not cause broad-range resistance to AMPs and classical antibiotics but can influence the efficiency of BamA inhibitors. MICs of the AMP polymyxin B ( A ), the antibiotic gentamicin ( B ), the BAM complex inhibitors MRL-494 ( C ), and darobactin ( D ) on the indicated strains were measured in triplicate. Dashed lines correspond to the average MIC of the wild-type. Continuous lines represent the average MIC for the three replicates.

Article Snippet: MRL-494 was provided by Medchemexpress and melittin by Enzo Life Sciences.

Techniques:

Deletion of non-essential components of the BAM complex does not result in resistance to TAT-RasGAP 317-326 but can increase sensitivity to diverse antimicrobial agents. MICs of TAT-RasGAP 317-326 ( A ), polymyxin B ( B ), melittin ( C ), gentamicin ( D ), vancomycin ( E ), MRL-494 ( F ), darobactin ( G ), meropenem ( H ), tetracycline ( I ), ciprofloxacin ( J ), and SDS ( K ) on the indicated deletion mutants from the Keio collection. MICs were measured in duplicate. Dashed lines correspond to the average MIC of the wild-type. Continuous lines represent the average MIC for the three replicates.

Journal: The Journal of Biological Chemistry

Article Title: Mutations in the essential outer membrane protein BamA contribute to Escherichia coli resistance to the antimicrobial peptide TAT-RasGAP 317-326

doi: 10.1016/j.jbc.2024.108018

Figure Lengend Snippet: Deletion of non-essential components of the BAM complex does not result in resistance to TAT-RasGAP 317-326 but can increase sensitivity to diverse antimicrobial agents. MICs of TAT-RasGAP 317-326 ( A ), polymyxin B ( B ), melittin ( C ), gentamicin ( D ), vancomycin ( E ), MRL-494 ( F ), darobactin ( G ), meropenem ( H ), tetracycline ( I ), ciprofloxacin ( J ), and SDS ( K ) on the indicated deletion mutants from the Keio collection. MICs were measured in duplicate. Dashed lines correspond to the average MIC of the wild-type. Continuous lines represent the average MIC for the three replicates.

Article Snippet: MRL-494 was provided by Medchemexpress and melittin by Enzo Life Sciences.

Techniques:

Journal: The Journal of Biological Chemistry

Article Title: Mutations in the essential outer membrane protein BamA contribute to Escherichia coli resistance to the antimicrobial peptide TAT-RasGAP 317-326

doi: 10.1016/j.jbc.2024.108018

Figure Lengend Snippet: BamA activity is only partially reduced in presence of TAT-RasGAP 317-326 . A , the known BamA inhibitor MRL-494 strongly reduces BamA and OmpC protein levels. Exponential phase wild-type E. coli were treated or not with 100 μM MRL-494 for 1 hour. OD 600 was then measured, and equivalent quantities of bacteria were harvested and resuspended in Laemmli buffer. A Western-blot was then performed with antibodies targeting BamA or OmpC, respectively. Analysis was performed using ImageJ and intensity of the bands was compared to the untreated control. Experiments were performed in duplicate; one representative result is shown and average of the two quantifications are provided. B , TAT-RasGAP 317-326 only partially reduces BamA and OmpC protein levels. Bacteria were treated or not with 32 μg/ml TAT-RasGAP 317-326 . Experiment was performed and analysed as for (A). C , protein profile is affected by TAT-RasGAP 317-326 treatment. Exponential phase bacteria were treated with the indicated concentrations of TAT-RasGAP 317-326 for the indicated periods of time. Equivalent quantities of bacteria (as determined by OD 600 measurement) were resuspended in Laemmli buffer and analysed by SDS-PAGE and Coomassie staining. Arrows indicate bands, which levels visibly decrease upon peptide treatment. Black line indicates separation between two different gels. D , TAT-RasGAP 317-326 treatment has a profound effect on protein composition of E. coli . Bacteria treated for 1 hour with or without 32 μg/ml TAT-RasGAP 317-326 were submitted to mass spectrometry analysis. Experiment was performed in duplicates and Fold-change of protein levels were determined. Proteins with Log 2 of fold change higher than two or lower than −2 are highlighted in red and green , respectively.

Article Snippet: MRL-494 was provided by Medchemexpress and melittin by Enzo Life Sciences.

Techniques: Activity Assay, Bacteria, Western Blot, Control, SDS Page, Staining, Mass Spectrometry

The combination between TAT-RasGAP 317-326 and the BAM complex inhibitor MRL-494 is synergistic. Checkerboard assay was performed using E. coli wild-type strain with the indicated concentrations of antimicrobial agents. Growth was monitored through OD 600 measurement and represented as a percentage of the untreated control. Synergism was determined with a FICI of 0.5, as detailed in the methods section. The experiment was performed in triplicate; a representative experiment is shown.

Journal: The Journal of Biological Chemistry

Article Title: Mutations in the essential outer membrane protein BamA contribute to Escherichia coli resistance to the antimicrobial peptide TAT-RasGAP 317-326

doi: 10.1016/j.jbc.2024.108018

Figure Lengend Snippet: The combination between TAT-RasGAP 317-326 and the BAM complex inhibitor MRL-494 is synergistic. Checkerboard assay was performed using E. coli wild-type strain with the indicated concentrations of antimicrobial agents. Growth was monitored through OD 600 measurement and represented as a percentage of the untreated control. Synergism was determined with a FICI of 0.5, as detailed in the methods section. The experiment was performed in triplicate; a representative experiment is shown.

Article Snippet: MRL-494 was provided by Medchemexpress and melittin by Enzo Life Sciences.

Techniques: Control

Review

sc 221947 melittin (MedChemExpress)

Structured Review

Images

Related Articles

Saline:

Reverse Transcription:

other:

Similar Products

Image Search Results