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rat cgrp  (MedChemExpress)


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    MedChemExpress rat cgrp
    Rat Cgrp, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hy-p0209/Rat+CGRP/pmc13168446-336-42-48
    Average 94 stars, based on 15 article reviews
    rat cgrp - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Bicinchoninic Acid Protein Assay:

    Article Title: CGRP alleviates lipopolysaccharide-induced ARDS inflammation via the HIF-1α signaling pathway
    Article Snippet: The present study aimed to investigate the effect of CGRP on the LPS-induced inflammatory response in ARDS and the role of HIF-1α in this process. .. LPS (Solarbio Biotechnology, Beijing, China); CGRP (MedChemexpress, New Jersey, U.S.A.); CGRP8-37 (MedChemexpress, New Jersey, U.S.A.); dimethyloxallyl glycine (DMOG, MedChemexpress, New Jersey, U.S.A.); BCA protein assay kit (Beyotime, Shanghai, China); superoxide dismutase (SOD) reagent detection kit (Nanjing Jiancheng Bioengineering Institute, China); malondialdehyde (MDA) reagent detection kit (Nanjing Jiancheng Bioengineering Institute, China); reactive oxygen species (ROS) reagent detection kit (Beyotime, Shanghai, China); IL-1β, TNF-α, IL-6, and CGRP ELISA test kit (Youkwei Biotechnology Co., LTD, Shanghai, China); dihydroethidium (Beyotime, Shanghai, China); bovine serum albumin (Sangon Biotech, Shanghai, China); anti-CD86 antibodies (ABclonal Technology, Wuhan, China); anti-CD206 antibodies (Affinity Biosciences, Changzhou, China) antibodies; secondary antibodies (Cy3: goat-anti-rabbit, Invitrogen, Carlsbad, CA, U.S.A.); FITC (goat-anti-mouse, Abcam, Cambridge, U.K); 4′, 6-diamidino-2-phenylindole (DAPI, Aladdin, Shanghai, China); Annexin V-FITC/PI apoptosis detection kit (Beyotime, Shanghai, China); FITC anti-mouse CD86 Antibody (Biolegend, California, U.S.A.); APC anti-mouse CD206 Antibody (Biolegend, California, U.S.A.), anti-CGRP (Santa Cruz Biotechnology, Texas, U.S.A., mouse mAb); anti-myeloperoxidase (MPO) (Proteintech, Wuhan, China, rabbit mAb); anti-receptor activity modifying protein 1 (RAMP1) (Proteintech, Wuhan, China, rabbit mAb), anti-HIF-1α (Cell Signaling Technology, Boston, U.S.A., rabbit mAb), and anti-GAPDH (Proteintech, Wuhan, China, rabbit mAb); WB secondary antibodies (Proteintech, Wuhan, China); Cell Counting Kit-8 (Beyotime, Shanghai, China). ..

    Multiple Displacement Amplification:

    Article Title: CGRP alleviates lipopolysaccharide-induced ARDS inflammation via the HIF-1α signaling pathway
    Article Snippet: The present study aimed to investigate the effect of CGRP on the LPS-induced inflammatory response in ARDS and the role of HIF-1α in this process. .. LPS (Solarbio Biotechnology, Beijing, China); CGRP (MedChemexpress, New Jersey, U.S.A.); CGRP8-37 (MedChemexpress, New Jersey, U.S.A.); dimethyloxallyl glycine (DMOG, MedChemexpress, New Jersey, U.S.A.); BCA protein assay kit (Beyotime, Shanghai, China); superoxide dismutase (SOD) reagent detection kit (Nanjing Jiancheng Bioengineering Institute, China); malondialdehyde (MDA) reagent detection kit (Nanjing Jiancheng Bioengineering Institute, China); reactive oxygen species (ROS) reagent detection kit (Beyotime, Shanghai, China); IL-1β, TNF-α, IL-6, and CGRP ELISA test kit (Youkwei Biotechnology Co., LTD, Shanghai, China); dihydroethidium (Beyotime, Shanghai, China); bovine serum albumin (Sangon Biotech, Shanghai, China); anti-CD86 antibodies (ABclonal Technology, Wuhan, China); anti-CD206 antibodies (Affinity Biosciences, Changzhou, China) antibodies; secondary antibodies (Cy3: goat-anti-rabbit, Invitrogen, Carlsbad, CA, U.S.A.); FITC (goat-anti-mouse, Abcam, Cambridge, U.K); 4′, 6-diamidino-2-phenylindole (DAPI, Aladdin, Shanghai, China); Annexin V-FITC/PI apoptosis detection kit (Beyotime, Shanghai, China); FITC anti-mouse CD86 Antibody (Biolegend, California, U.S.A.); APC anti-mouse CD206 Antibody (Biolegend, California, U.S.A.), anti-CGRP (Santa Cruz Biotechnology, Texas, U.S.A., mouse mAb); anti-myeloperoxidase (MPO) (Proteintech, Wuhan, China, rabbit mAb); anti-receptor activity modifying protein 1 (RAMP1) (Proteintech, Wuhan, China, rabbit mAb), anti-HIF-1α (Cell Signaling Technology, Boston, U.S.A., rabbit mAb), and anti-GAPDH (Proteintech, Wuhan, China, rabbit mAb); WB secondary antibodies (Proteintech, Wuhan, China); Cell Counting Kit-8 (Beyotime, Shanghai, China). ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: CGRP alleviates lipopolysaccharide-induced ARDS inflammation via the HIF-1α signaling pathway
    Article Snippet: The present study aimed to investigate the effect of CGRP on the LPS-induced inflammatory response in ARDS and the role of HIF-1α in this process. .. LPS (Solarbio Biotechnology, Beijing, China); CGRP (MedChemexpress, New Jersey, U.S.A.); CGRP8-37 (MedChemexpress, New Jersey, U.S.A.); dimethyloxallyl glycine (DMOG, MedChemexpress, New Jersey, U.S.A.); BCA protein assay kit (Beyotime, Shanghai, China); superoxide dismutase (SOD) reagent detection kit (Nanjing Jiancheng Bioengineering Institute, China); malondialdehyde (MDA) reagent detection kit (Nanjing Jiancheng Bioengineering Institute, China); reactive oxygen species (ROS) reagent detection kit (Beyotime, Shanghai, China); IL-1β, TNF-α, IL-6, and CGRP ELISA test kit (Youkwei Biotechnology Co., LTD, Shanghai, China); dihydroethidium (Beyotime, Shanghai, China); bovine serum albumin (Sangon Biotech, Shanghai, China); anti-CD86 antibodies (ABclonal Technology, Wuhan, China); anti-CD206 antibodies (Affinity Biosciences, Changzhou, China) antibodies; secondary antibodies (Cy3: goat-anti-rabbit, Invitrogen, Carlsbad, CA, U.S.A.); FITC (goat-anti-mouse, Abcam, Cambridge, U.K); 4′, 6-diamidino-2-phenylindole (DAPI, Aladdin, Shanghai, China); Annexin V-FITC/PI apoptosis detection kit (Beyotime, Shanghai, China); FITC anti-mouse CD86 Antibody (Biolegend, California, U.S.A.); APC anti-mouse CD206 Antibody (Biolegend, California, U.S.A.), anti-CGRP (Santa Cruz Biotechnology, Texas, U.S.A., mouse mAb); anti-myeloperoxidase (MPO) (Proteintech, Wuhan, China, rabbit mAb); anti-receptor activity modifying protein 1 (RAMP1) (Proteintech, Wuhan, China, rabbit mAb), anti-HIF-1α (Cell Signaling Technology, Boston, U.S.A., rabbit mAb), and anti-GAPDH (Proteintech, Wuhan, China, rabbit mAb); WB secondary antibodies (Proteintech, Wuhan, China); Cell Counting Kit-8 (Beyotime, Shanghai, China). ..

    Article Title: Low-intensity focused ultrasound-activated piezoelectric gel bandage for diabetic wound repair and neuropathic pain relief
    Article Snippet: Calcium ion indicator (Fluo-4 AM), Griess reagent kit and DCFH-DA were obtained from Beyotime. .. Streptozotocin (STZ, ≥98%) was purchased from Maokang Biotech Co., Ltd. Rat VEGF and EGF ELISA kits were acquired from Enzyme-linked Biotech Co., Ltd. Rat TNF-α, IL-10, IL-6 and CGRP ELISA kits were acquired from Multi Sciences Biotech Co., Ltd. α-CGRP (99.8%) and rat CGRP-(8-37) (99%) were purchased from MedChemExpress. ..

    Activity Assay:

    Article Title: CGRP alleviates lipopolysaccharide-induced ARDS inflammation via the HIF-1α signaling pathway
    Article Snippet: The present study aimed to investigate the effect of CGRP on the LPS-induced inflammatory response in ARDS and the role of HIF-1α in this process. .. LPS (Solarbio Biotechnology, Beijing, China); CGRP (MedChemexpress, New Jersey, U.S.A.); CGRP8-37 (MedChemexpress, New Jersey, U.S.A.); dimethyloxallyl glycine (DMOG, MedChemexpress, New Jersey, U.S.A.); BCA protein assay kit (Beyotime, Shanghai, China); superoxide dismutase (SOD) reagent detection kit (Nanjing Jiancheng Bioengineering Institute, China); malondialdehyde (MDA) reagent detection kit (Nanjing Jiancheng Bioengineering Institute, China); reactive oxygen species (ROS) reagent detection kit (Beyotime, Shanghai, China); IL-1β, TNF-α, IL-6, and CGRP ELISA test kit (Youkwei Biotechnology Co., LTD, Shanghai, China); dihydroethidium (Beyotime, Shanghai, China); bovine serum albumin (Sangon Biotech, Shanghai, China); anti-CD86 antibodies (ABclonal Technology, Wuhan, China); anti-CD206 antibodies (Affinity Biosciences, Changzhou, China) antibodies; secondary antibodies (Cy3: goat-anti-rabbit, Invitrogen, Carlsbad, CA, U.S.A.); FITC (goat-anti-mouse, Abcam, Cambridge, U.K); 4′, 6-diamidino-2-phenylindole (DAPI, Aladdin, Shanghai, China); Annexin V-FITC/PI apoptosis detection kit (Beyotime, Shanghai, China); FITC anti-mouse CD86 Antibody (Biolegend, California, U.S.A.); APC anti-mouse CD206 Antibody (Biolegend, California, U.S.A.), anti-CGRP (Santa Cruz Biotechnology, Texas, U.S.A., mouse mAb); anti-myeloperoxidase (MPO) (Proteintech, Wuhan, China, rabbit mAb); anti-receptor activity modifying protein 1 (RAMP1) (Proteintech, Wuhan, China, rabbit mAb), anti-HIF-1α (Cell Signaling Technology, Boston, U.S.A., rabbit mAb), and anti-GAPDH (Proteintech, Wuhan, China, rabbit mAb); WB secondary antibodies (Proteintech, Wuhan, China); Cell Counting Kit-8 (Beyotime, Shanghai, China). ..

    Western Blot:

    Article Title: CGRP alleviates lipopolysaccharide-induced ARDS inflammation via the HIF-1α signaling pathway
    Article Snippet: The present study aimed to investigate the effect of CGRP on the LPS-induced inflammatory response in ARDS and the role of HIF-1α in this process. .. LPS (Solarbio Biotechnology, Beijing, China); CGRP (MedChemexpress, New Jersey, U.S.A.); CGRP8-37 (MedChemexpress, New Jersey, U.S.A.); dimethyloxallyl glycine (DMOG, MedChemexpress, New Jersey, U.S.A.); BCA protein assay kit (Beyotime, Shanghai, China); superoxide dismutase (SOD) reagent detection kit (Nanjing Jiancheng Bioengineering Institute, China); malondialdehyde (MDA) reagent detection kit (Nanjing Jiancheng Bioengineering Institute, China); reactive oxygen species (ROS) reagent detection kit (Beyotime, Shanghai, China); IL-1β, TNF-α, IL-6, and CGRP ELISA test kit (Youkwei Biotechnology Co., LTD, Shanghai, China); dihydroethidium (Beyotime, Shanghai, China); bovine serum albumin (Sangon Biotech, Shanghai, China); anti-CD86 antibodies (ABclonal Technology, Wuhan, China); anti-CD206 antibodies (Affinity Biosciences, Changzhou, China) antibodies; secondary antibodies (Cy3: goat-anti-rabbit, Invitrogen, Carlsbad, CA, U.S.A.); FITC (goat-anti-mouse, Abcam, Cambridge, U.K); 4′, 6-diamidino-2-phenylindole (DAPI, Aladdin, Shanghai, China); Annexin V-FITC/PI apoptosis detection kit (Beyotime, Shanghai, China); FITC anti-mouse CD86 Antibody (Biolegend, California, U.S.A.); APC anti-mouse CD206 Antibody (Biolegend, California, U.S.A.), anti-CGRP (Santa Cruz Biotechnology, Texas, U.S.A., mouse mAb); anti-myeloperoxidase (MPO) (Proteintech, Wuhan, China, rabbit mAb); anti-receptor activity modifying protein 1 (RAMP1) (Proteintech, Wuhan, China, rabbit mAb), anti-HIF-1α (Cell Signaling Technology, Boston, U.S.A., rabbit mAb), and anti-GAPDH (Proteintech, Wuhan, China, rabbit mAb); WB secondary antibodies (Proteintech, Wuhan, China); Cell Counting Kit-8 (Beyotime, Shanghai, China). ..

    CCK-8 Assay:

    Article Title: CGRP alleviates lipopolysaccharide-induced ARDS inflammation via the HIF-1α signaling pathway
    Article Snippet: The present study aimed to investigate the effect of CGRP on the LPS-induced inflammatory response in ARDS and the role of HIF-1α in this process. .. LPS (Solarbio Biotechnology, Beijing, China); CGRP (MedChemexpress, New Jersey, U.S.A.); CGRP8-37 (MedChemexpress, New Jersey, U.S.A.); dimethyloxallyl glycine (DMOG, MedChemexpress, New Jersey, U.S.A.); BCA protein assay kit (Beyotime, Shanghai, China); superoxide dismutase (SOD) reagent detection kit (Nanjing Jiancheng Bioengineering Institute, China); malondialdehyde (MDA) reagent detection kit (Nanjing Jiancheng Bioengineering Institute, China); reactive oxygen species (ROS) reagent detection kit (Beyotime, Shanghai, China); IL-1β, TNF-α, IL-6, and CGRP ELISA test kit (Youkwei Biotechnology Co., LTD, Shanghai, China); dihydroethidium (Beyotime, Shanghai, China); bovine serum albumin (Sangon Biotech, Shanghai, China); anti-CD86 antibodies (ABclonal Technology, Wuhan, China); anti-CD206 antibodies (Affinity Biosciences, Changzhou, China) antibodies; secondary antibodies (Cy3: goat-anti-rabbit, Invitrogen, Carlsbad, CA, U.S.A.); FITC (goat-anti-mouse, Abcam, Cambridge, U.K); 4′, 6-diamidino-2-phenylindole (DAPI, Aladdin, Shanghai, China); Annexin V-FITC/PI apoptosis detection kit (Beyotime, Shanghai, China); FITC anti-mouse CD86 Antibody (Biolegend, California, U.S.A.); APC anti-mouse CD206 Antibody (Biolegend, California, U.S.A.), anti-CGRP (Santa Cruz Biotechnology, Texas, U.S.A., mouse mAb); anti-myeloperoxidase (MPO) (Proteintech, Wuhan, China, rabbit mAb); anti-receptor activity modifying protein 1 (RAMP1) (Proteintech, Wuhan, China, rabbit mAb), anti-HIF-1α (Cell Signaling Technology, Boston, U.S.A., rabbit mAb), and anti-GAPDH (Proteintech, Wuhan, China, rabbit mAb); WB secondary antibodies (Proteintech, Wuhan, China); Cell Counting Kit-8 (Beyotime, Shanghai, China). ..

    Saline:

    Article Title: CGRP alleviates lipopolysaccharide-induced ARDS inflammation via the HIF-1α signaling pathway
    Article Snippet: .. In this study, CGRP (MCE, U.S.A.), CGRP8-37 (MCE, U.S.A.), and DMOG (MCE, U.S.A.) were dissolved in saline. ..

    other:

    Article Title: Low-intensity focused ultrasound-activated piezoelectric gel bandage for diabetic wound repair and neuropathic pain relief.
    Article Snippet: Barium titanate (BT, 99.9%), NONOate (98%), polyethyleneimine (PEI, 99%) and polyvinyl alcohol (PVA, 99%) were obtained from Rhawn.



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    CGRP protects BMSCs from serum deprivation-induced apoptosis: (A) representative flow cytometric dot plots showing Annexin V-FITC/PI double staining after 24 and 48 h of serum-free culture. Four distinct cell populations are displayed: viable cells (Annexin V − /PI − , lower left quadrant), early apoptotic cells (Annexin V + /PI − , lower right quadrant), late apoptotic cells (Annexin V + /PI + , upper right quadrant), and necrotic cells (Annexin V − /PI + , upper left quadrant). Representative plots show control, CGRP (10 −8 M), CGRP <t>+</t> <t>CGRP8-37</t> (10 −8 M CGRP + 10 −6 M CGRP8-37), and CGRP + H89 (10 −8 M CGRP + 0.2 μg/mL H89) treatment groups. (B) Quantitative analysis of the total apoptotic rate calculated as the sum of early apoptotic (Annexin V + /PI − ) and late apoptotic (Annexin V + /PI + ) cell percentages. CGRP treatment significantly reduced total apoptosis compared to control. CGRP8-37 and H89 co-treatments abolished CGRP’s protective effects, with apoptosis rates returning to control levels. Data represent mean ± SD from independent experiments (n = 6). *P < 0.05 compared to the control group; *P < 0.05 compared to the CGRP group using one-way ANOVA followed by Tukey’s post hoc test.
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    Image Search Results


    CGRP protects BMSCs from serum deprivation-induced apoptosis: (A) representative flow cytometric dot plots showing Annexin V-FITC/PI double staining after 24 and 48 h of serum-free culture. Four distinct cell populations are displayed: viable cells (Annexin V − /PI − , lower left quadrant), early apoptotic cells (Annexin V + /PI − , lower right quadrant), late apoptotic cells (Annexin V + /PI + , upper right quadrant), and necrotic cells (Annexin V − /PI + , upper left quadrant). Representative plots show control, CGRP (10 −8 M), CGRP + CGRP8-37 (10 −8 M CGRP + 10 −6 M CGRP8-37), and CGRP + H89 (10 −8 M CGRP + 0.2 μg/mL H89) treatment groups. (B) Quantitative analysis of the total apoptotic rate calculated as the sum of early apoptotic (Annexin V + /PI − ) and late apoptotic (Annexin V + /PI + ) cell percentages. CGRP treatment significantly reduced total apoptosis compared to control. CGRP8-37 and H89 co-treatments abolished CGRP’s protective effects, with apoptosis rates returning to control levels. Data represent mean ± SD from independent experiments (n = 6). *P < 0.05 compared to the control group; *P < 0.05 compared to the CGRP group using one-way ANOVA followed by Tukey’s post hoc test.

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: CGRP-dependent molecular signaling drives bone marrow stem cell osteogenesis in distraction osteogenesis

    doi: 10.3389/fbioe.2025.1641476

    Figure Lengend Snippet: CGRP protects BMSCs from serum deprivation-induced apoptosis: (A) representative flow cytometric dot plots showing Annexin V-FITC/PI double staining after 24 and 48 h of serum-free culture. Four distinct cell populations are displayed: viable cells (Annexin V − /PI − , lower left quadrant), early apoptotic cells (Annexin V + /PI − , lower right quadrant), late apoptotic cells (Annexin V + /PI + , upper right quadrant), and necrotic cells (Annexin V − /PI + , upper left quadrant). Representative plots show control, CGRP (10 −8 M), CGRP + CGRP8-37 (10 −8 M CGRP + 10 −6 M CGRP8-37), and CGRP + H89 (10 −8 M CGRP + 0.2 μg/mL H89) treatment groups. (B) Quantitative analysis of the total apoptotic rate calculated as the sum of early apoptotic (Annexin V + /PI − ) and late apoptotic (Annexin V + /PI + ) cell percentages. CGRP treatment significantly reduced total apoptosis compared to control. CGRP8-37 and H89 co-treatments abolished CGRP’s protective effects, with apoptosis rates returning to control levels. Data represent mean ± SD from independent experiments (n = 6). *P < 0.05 compared to the control group; *P < 0.05 compared to the CGRP group using one-way ANOVA followed by Tukey’s post hoc test.

    Article Snippet: Rat α-calcitonin gene-related peptide (rat α-CGRP, HY-P0203), CGRP receptor antagonist rat CGRP8-37 (HY-P0209), and PKA inhibitor H89 (HY-15979) were purchased from MCE (China).

    Techniques: Double Staining, Control

    CGRP enhances BMSC osteogenic differentiation: (A) Alizarin Red S staining showing calcium nodule formation at days 14 and 21 of osteogenic induction. CGRP treatment resulted in more numerous and deeper-stained orange-red calcified nodules compared to controls. CGRP8-37 and H89 treatments significantly reduced CGRP-induced mineralization enhancement (scale bars: 50 μm). (B) Quantitative analysis of Alizarin Red staining using the cetylpyridinium chloride (CPC) extraction method. CGRP treatment showed 3.7-fold and 3.5-fold increases in mineralization at 14 and 21 days, respectively, compared to controls. Both CGRP8-37 and H89 treatments effectively blocked CGRP-induced mineralization enhancement. (C) Alkaline phosphatase (ALP) staining demonstrating enhanced early osteogenic marker expression at days 4 and 7. Purple precipitates indicate ALP activity (scale bars: 50 μm). CGRP8-37 and H89 treatments inhibited CGRP-induced osteogenic enhancement. (D) Quantitative ALP activity measurements normalized to total protein content. CGRP treatment demonstrated 3.7-fold and 3.9-fold increases in ALP activity at 4 and 7 days, respectively, compared to controls. CGRP + CGRP8-37 and CGRP + H89 groups showed significantly reduced ALP activity compared to CGRP alone, confirming that both receptor blockade and PKA pathway inhibition prevent CGRP’s osteogenic enhancing effects.

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: CGRP-dependent molecular signaling drives bone marrow stem cell osteogenesis in distraction osteogenesis

    doi: 10.3389/fbioe.2025.1641476

    Figure Lengend Snippet: CGRP enhances BMSC osteogenic differentiation: (A) Alizarin Red S staining showing calcium nodule formation at days 14 and 21 of osteogenic induction. CGRP treatment resulted in more numerous and deeper-stained orange-red calcified nodules compared to controls. CGRP8-37 and H89 treatments significantly reduced CGRP-induced mineralization enhancement (scale bars: 50 μm). (B) Quantitative analysis of Alizarin Red staining using the cetylpyridinium chloride (CPC) extraction method. CGRP treatment showed 3.7-fold and 3.5-fold increases in mineralization at 14 and 21 days, respectively, compared to controls. Both CGRP8-37 and H89 treatments effectively blocked CGRP-induced mineralization enhancement. (C) Alkaline phosphatase (ALP) staining demonstrating enhanced early osteogenic marker expression at days 4 and 7. Purple precipitates indicate ALP activity (scale bars: 50 μm). CGRP8-37 and H89 treatments inhibited CGRP-induced osteogenic enhancement. (D) Quantitative ALP activity measurements normalized to total protein content. CGRP treatment demonstrated 3.7-fold and 3.9-fold increases in ALP activity at 4 and 7 days, respectively, compared to controls. CGRP + CGRP8-37 and CGRP + H89 groups showed significantly reduced ALP activity compared to CGRP alone, confirming that both receptor blockade and PKA pathway inhibition prevent CGRP’s osteogenic enhancing effects.

    Article Snippet: Rat α-calcitonin gene-related peptide (rat α-CGRP, HY-P0203), CGRP receptor antagonist rat CGRP8-37 (HY-P0209), and PKA inhibitor H89 (HY-15979) were purchased from MCE (China).

    Techniques: Staining, Extraction, Marker, Expressing, Activity Assay, Inhibition

    CGRP activates the cAMP/PKA signaling pathway in BMSCs: (A) ELISA quantification of intracellular cAMP levels 30 min post-treatment. CGRP rapidly increased cAMP concentration, blocked by CGRP8-37 and H89. Data represent mean ± SD (n = 6). (B-D) Western blot analysis of PKA and CREB phosphorylation at 30 min. Representative blots and quantitative analysis of p-PKA/PKA and p-CREB/CREB ratios. Data represent mean ± SD. *P < 0.05.

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: CGRP-dependent molecular signaling drives bone marrow stem cell osteogenesis in distraction osteogenesis

    doi: 10.3389/fbioe.2025.1641476

    Figure Lengend Snippet: CGRP activates the cAMP/PKA signaling pathway in BMSCs: (A) ELISA quantification of intracellular cAMP levels 30 min post-treatment. CGRP rapidly increased cAMP concentration, blocked by CGRP8-37 and H89. Data represent mean ± SD (n = 6). (B-D) Western blot analysis of PKA and CREB phosphorylation at 30 min. Representative blots and quantitative analysis of p-PKA/PKA and p-CREB/CREB ratios. Data represent mean ± SD. *P < 0.05.

    Article Snippet: Rat α-calcitonin gene-related peptide (rat α-CGRP, HY-P0203), CGRP receptor antagonist rat CGRP8-37 (HY-P0209), and PKA inhibitor H89 (HY-15979) were purchased from MCE (China).

    Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay, Western Blot, Phospho-proteomics

    CGRP enhances biomechanical properties of regenerated bone: three-point bending test results at 6 weeks of consolidation showing (A) ultimate load, (B) energy to failure, and (C) stiffness measurements. CGRP treatment significantly improved all mechanical parameters compared to controls. CGRP8-37 and H89 abolished these beneficial effects. Data represent mean ± SD. *P < 0.05.

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: CGRP-dependent molecular signaling drives bone marrow stem cell osteogenesis in distraction osteogenesis

    doi: 10.3389/fbioe.2025.1641476

    Figure Lengend Snippet: CGRP enhances biomechanical properties of regenerated bone: three-point bending test results at 6 weeks of consolidation showing (A) ultimate load, (B) energy to failure, and (C) stiffness measurements. CGRP treatment significantly improved all mechanical parameters compared to controls. CGRP8-37 and H89 abolished these beneficial effects. Data represent mean ± SD. *P < 0.05.

    Article Snippet: Rat α-calcitonin gene-related peptide (rat α-CGRP, HY-P0203), CGRP receptor antagonist rat CGRP8-37 (HY-P0209), and PKA inhibitor H89 (HY-15979) were purchased from MCE (China).

    Techniques: