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npy  (MedChemExpress)


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    Structured Review

    MedChemExpress npy
    A In situ hybridization RNAscope images of MOR mRNA ( Oprm1 , white) and <t>NPY</t> mRNA ( Npy , red) in mouse spinal dorsal horn (SDH). Yellow arrows indicate Oprm1 double-labeled with Npy , cells containing ≥ 5 RNAscope puncta were considered positive expression. Scale bar = 20 μm. B The percentage of co-expression between MOR and NPY neurons in the SDH, 12 spinal cord sections from 6 mice were analyzed. C Time course (left) and total scratch bouts (right) following intrathecal injection 10 <t>nmol</t> <t>CNO</t> (30 min before) and 0.3 nmol morphine in Npy Cre ;hM3Dq or WT mice, student’s unpaired two-tailed t -test. D Time course (left) and total scratch bouts (right) after intrathecal injection of 0.1, 0.3, 1 nmol morphine in Npy Cre ;Oprm1 fl/fl mice, two-way ANOVA, Bonferroni’s multiple comparisons. E Time course (left) and total scratch bouts (right) after intrathecal injection of various doses of EM-1 (0.3, 1, 3, 10 nmol) in WT mice. One-way ANOVA, Dunnett’s multiple comparisons. F Intrathecal EM-1 (10 nmol)-induced scratching was significantly inhibited in Npy Cre ;Oprm1 fl/fl mice, student’s unpaired two-tailed t test. Data are shown as means ± SEM. P values are indicated in the figures. Sample sizes are presented in parentheses. Source data are provided as a Source Data file. Created in BioRender. Wang, Z. (2025) https://BioRender.com/u0x7q3a .
    Npy, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hy-p0198a/Neuropeptide+Y/pmc12317128-335-16-17
    Average 93 stars, based on 2 article reviews
    npy - by Bioz Stars, 2026-10
    93/100 stars

    Images

    1) Product Images from "Neuropeptide Y neurons mediate opioid-induced itch by disinhibiting GRP-GRPR microcircuits in the spinal cord"

    Article Title: Neuropeptide Y neurons mediate opioid-induced itch by disinhibiting GRP-GRPR microcircuits in the spinal cord

    Journal: Nature Communications

    doi: 10.1038/s41467-025-62382-w

    A In situ hybridization RNAscope images of MOR mRNA ( Oprm1 , white) and NPY mRNA ( Npy , red) in mouse spinal dorsal horn (SDH). Yellow arrows indicate Oprm1 double-labeled with Npy , cells containing ≥ 5 RNAscope puncta were considered positive expression. Scale bar = 20 μm. B The percentage of co-expression between MOR and NPY neurons in the SDH, 12 spinal cord sections from 6 mice were analyzed. C Time course (left) and total scratch bouts (right) following intrathecal injection 10 nmol CNO (30 min before) and 0.3 nmol morphine in Npy Cre ;hM3Dq or WT mice, student’s unpaired two-tailed t -test. D Time course (left) and total scratch bouts (right) after intrathecal injection of 0.1, 0.3, 1 nmol morphine in Npy Cre ;Oprm1 fl/fl mice, two-way ANOVA, Bonferroni’s multiple comparisons. E Time course (left) and total scratch bouts (right) after intrathecal injection of various doses of EM-1 (0.3, 1, 3, 10 nmol) in WT mice. One-way ANOVA, Dunnett’s multiple comparisons. F Intrathecal EM-1 (10 nmol)-induced scratching was significantly inhibited in Npy Cre ;Oprm1 fl/fl mice, student’s unpaired two-tailed t test. Data are shown as means ± SEM. P values are indicated in the figures. Sample sizes are presented in parentheses. Source data are provided as a Source Data file. Created in BioRender. Wang, Z. (2025) https://BioRender.com/u0x7q3a .
    Figure Legend Snippet: A In situ hybridization RNAscope images of MOR mRNA ( Oprm1 , white) and NPY mRNA ( Npy , red) in mouse spinal dorsal horn (SDH). Yellow arrows indicate Oprm1 double-labeled with Npy , cells containing ≥ 5 RNAscope puncta were considered positive expression. Scale bar = 20 μm. B The percentage of co-expression between MOR and NPY neurons in the SDH, 12 spinal cord sections from 6 mice were analyzed. C Time course (left) and total scratch bouts (right) following intrathecal injection 10 nmol CNO (30 min before) and 0.3 nmol morphine in Npy Cre ;hM3Dq or WT mice, student’s unpaired two-tailed t -test. D Time course (left) and total scratch bouts (right) after intrathecal injection of 0.1, 0.3, 1 nmol morphine in Npy Cre ;Oprm1 fl/fl mice, two-way ANOVA, Bonferroni’s multiple comparisons. E Time course (left) and total scratch bouts (right) after intrathecal injection of various doses of EM-1 (0.3, 1, 3, 10 nmol) in WT mice. One-way ANOVA, Dunnett’s multiple comparisons. F Intrathecal EM-1 (10 nmol)-induced scratching was significantly inhibited in Npy Cre ;Oprm1 fl/fl mice, student’s unpaired two-tailed t test. Data are shown as means ± SEM. P values are indicated in the figures. Sample sizes are presented in parentheses. Source data are provided as a Source Data file. Created in BioRender. Wang, Z. (2025) https://BioRender.com/u0x7q3a .

    Techniques Used: In Situ Hybridization, RNAscope, Labeling, Expressing, Injection, Two Tailed Test

    A Intrathecal morphine-induced scratching was dose-dependently inhibited by co-injection with NPY (1, 3, 10 μg), one-way ANOVA, Dunnett’s multiple comparisons. B Intrathecal morphine-induced scratching was significantly attenuated by co-injection with NPY1R selective agonist [Leu 31 , Pro 34 ] NPY (LP-NPY) (0.3, 1, 3 μg), one-way ANOVA, Dunnett’s multiple comparisons. C Intrathecal injection of NPY1R selective antagonist BIBO3304 (BIBO, 10 μg, 20 min before) blocked the effects of LP-NPY (3 μg) on morphine-induced itch, one-way ANOVA, Dunnett’s multiple comparisons. D Intrathecal co-injected morphine (0.3 nmol) and NPY (10 μg) in WT or Npy1r −/− mice, one-way ANOVA, Dunnett’s multiple comparisons. E Intrathecal EM-1 (10 nmol)-induced scratching was inhibited by 10 μg NPY, student’s unpaired two-tailed t -test. F Intrathecal EM-1 (10 nmol)-induced scratching was inhibited by 3 μg LP-NPY, student’s unpaired two-tailed t -test. Data are shown as means ± SEM. P values are indicated in the figures. Sample sizes are presented in parentheses. Source data are provided as a Source Data file.
    Figure Legend Snippet: A Intrathecal morphine-induced scratching was dose-dependently inhibited by co-injection with NPY (1, 3, 10 μg), one-way ANOVA, Dunnett’s multiple comparisons. B Intrathecal morphine-induced scratching was significantly attenuated by co-injection with NPY1R selective agonist [Leu 31 , Pro 34 ] NPY (LP-NPY) (0.3, 1, 3 μg), one-way ANOVA, Dunnett’s multiple comparisons. C Intrathecal injection of NPY1R selective antagonist BIBO3304 (BIBO, 10 μg, 20 min before) blocked the effects of LP-NPY (3 μg) on morphine-induced itch, one-way ANOVA, Dunnett’s multiple comparisons. D Intrathecal co-injected morphine (0.3 nmol) and NPY (10 μg) in WT or Npy1r −/− mice, one-way ANOVA, Dunnett’s multiple comparisons. E Intrathecal EM-1 (10 nmol)-induced scratching was inhibited by 10 μg NPY, student’s unpaired two-tailed t -test. F Intrathecal EM-1 (10 nmol)-induced scratching was inhibited by 3 μg LP-NPY, student’s unpaired two-tailed t -test. Data are shown as means ± SEM. P values are indicated in the figures. Sample sizes are presented in parentheses. Source data are provided as a Source Data file.

    Techniques Used: Injection, Two Tailed Test

    A Representative traces of action potentials before and after NPY (10 μM) treatment in GRP + interneurons in the spinal slices from Grp Cre ;Ai9 mice. B Quantifications of the effects of NPY on action potentials, two-way ANOVA, Bonferroni’s multiple comparisons. 8 neurons from 3 mice were analyzed. C Quantifications of the rheobase, paired two-tailed t -test. 8 neurons from 3 mice were analyzed. D Representative traces of action potentials before and after vehicle treatment in Grp + interneurons in the spinal slices from Grp Cre ;Ai9 mice. E Quantifications of the effects of the vehicle on action potentials, two-way ANOVA, Bonferroni’s multiple comparisons.10 neurons from 3 mice were analyzed. F Quantifications of the rheobase, paired two-tailed t -test. Data are shown as means with dots. 10 neurons from 3 mice were analyzed. G NPY (10 μM) induced outward currents were recorded and inhibited by BIBO3304(2 μM) from GRP + interneurons in the SDH from Grp Cre ;Ai9 mice. 19 neurons from 3 mice and 12 neurons from 3 mice were analyzed, respectively. Student’s unpaired two-tailed t -test. H Time course (left) and total scratch bouts (right) following intrathecal CNO (10 nmol) and NPY (10 μg) injection in Grp Cre ;hM3Dq , student’s unpaired two-tailed t -test. Data are shown as means ± SEM. P values are indicated in the figures. Sample sizes are presented in parentheses. Source data are provided as a Source Data file. Created in BioRender. Wang, Z. (2025) https://BioRender.com/ox4ft8z .
    Figure Legend Snippet: A Representative traces of action potentials before and after NPY (10 μM) treatment in GRP + interneurons in the spinal slices from Grp Cre ;Ai9 mice. B Quantifications of the effects of NPY on action potentials, two-way ANOVA, Bonferroni’s multiple comparisons. 8 neurons from 3 mice were analyzed. C Quantifications of the rheobase, paired two-tailed t -test. 8 neurons from 3 mice were analyzed. D Representative traces of action potentials before and after vehicle treatment in Grp + interneurons in the spinal slices from Grp Cre ;Ai9 mice. E Quantifications of the effects of the vehicle on action potentials, two-way ANOVA, Bonferroni’s multiple comparisons.10 neurons from 3 mice were analyzed. F Quantifications of the rheobase, paired two-tailed t -test. Data are shown as means with dots. 10 neurons from 3 mice were analyzed. G NPY (10 μM) induced outward currents were recorded and inhibited by BIBO3304(2 μM) from GRP + interneurons in the SDH from Grp Cre ;Ai9 mice. 19 neurons from 3 mice and 12 neurons from 3 mice were analyzed, respectively. Student’s unpaired two-tailed t -test. H Time course (left) and total scratch bouts (right) following intrathecal CNO (10 nmol) and NPY (10 μg) injection in Grp Cre ;hM3Dq , student’s unpaired two-tailed t -test. Data are shown as means ± SEM. P values are indicated in the figures. Sample sizes are presented in parentheses. Source data are provided as a Source Data file. Created in BioRender. Wang, Z. (2025) https://BioRender.com/ox4ft8z .

    Techniques Used: Two Tailed Test, Injection

    Related Articles

    Control:

    Article Title: Intermittent Fasting Targets Osteocyte Neuropeptide Y to Relieve Osteoarthritis.
    Article Snippet: .. For MM-CM preparation, the culture of RAW264.7 cells was added with OCYCM from different groups, NPY (0.1 nm; Cat. No. HY-P0198A; MedChemExpress, Monmouth Junction, USA) + un-cultured control medium of osteocytes (NPY group for short), or vehicle of NPY + un-cultured control medium of osteocytes (Control group for short). ..

    other:

    Article Title: Neuropeptide Y neurons mediate opioid-induced itch by disinhibiting GRP-GRPR microcircuits in the spinal cord.
    Article Snippet: For intrathecal injections, lumbar puncture was made by a Hamilton microsyringe (Hamilton) fitted with a 30-G needle between the L5 and L6 spinal levels to deliver reagents (10μl) into CSF in non-anesthetized mice.



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    doi: 10.1002/advs.202400196

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    Techniques: Immunohistochemical staining, Staining, Concentration Assay, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Western Blot, Two Tailed Test, Control