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reacp53 treatment  (MedChemExpress)


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    MedChemExpress reacp53 treatment
    Fig. 4. Silence of linc00023 inhibited pyroptosis and promoted cell proliferation via regulating p53 expression in ACHN cells. (A) qRT-PCR was applied to monitor p53 and caspase-1 levels in three groups, including si-NC, si-linc00023, and si-linc00023 + <t>ReACp53.</t> N = 3. (B) Western blot was applied to evaluate the expression of caspase-1, GSDMD, NLRP3 and p53 in three groups, including si-NC, si-linc00023, and si-linc00023 + ReACp53. N = 3. (C&D) ELISA was utilized to test IL-1β (C) and IL-18 (D) concentrations in supernatant from three groups, including si-NC, si-linc00023, and si-linc00023 + ReACp53. N = 3. (E) Western blot was used to detect the expression of IL-1β and IL-18 in the groups of si-NC, si-linc00023, and si-linc00023 + ReACp53. N = 3. (F) MTS was applied to measure the following three groups’ cell proliferation abilities at 24, 48, and 72 h. N = 3. **P < 0.01, ****P < 0.0001.
    Reacp53 Treatment, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hy-p0121/ReACp53/pm37429368-55-1-4
    Average 91 stars, based on 2 article reviews
    reacp53 treatment - by Bioz Stars, 2026-10
    91/100 stars

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    1) Product Images from "Silence of linc00023 inhibits pyroptosis and promotes cell proliferation via regulating p53."

    Article Title: Silence of linc00023 inhibits pyroptosis and promotes cell proliferation via regulating p53.

    Journal: Gene

    doi: 10.1016/j.gene.2023.147628

    Fig. 4. Silence of linc00023 inhibited pyroptosis and promoted cell proliferation via regulating p53 expression in ACHN cells. (A) qRT-PCR was applied to monitor p53 and caspase-1 levels in three groups, including si-NC, si-linc00023, and si-linc00023 + ReACp53. N = 3. (B) Western blot was applied to evaluate the expression of caspase-1, GSDMD, NLRP3 and p53 in three groups, including si-NC, si-linc00023, and si-linc00023 + ReACp53. N = 3. (C&D) ELISA was utilized to test IL-1β (C) and IL-18 (D) concentrations in supernatant from three groups, including si-NC, si-linc00023, and si-linc00023 + ReACp53. N = 3. (E) Western blot was used to detect the expression of IL-1β and IL-18 in the groups of si-NC, si-linc00023, and si-linc00023 + ReACp53. N = 3. (F) MTS was applied to measure the following three groups’ cell proliferation abilities at 24, 48, and 72 h. N = 3. **P < 0.01, ****P < 0.0001.
    Figure Legend Snippet: Fig. 4. Silence of linc00023 inhibited pyroptosis and promoted cell proliferation via regulating p53 expression in ACHN cells. (A) qRT-PCR was applied to monitor p53 and caspase-1 levels in three groups, including si-NC, si-linc00023, and si-linc00023 + ReACp53. N = 3. (B) Western blot was applied to evaluate the expression of caspase-1, GSDMD, NLRP3 and p53 in three groups, including si-NC, si-linc00023, and si-linc00023 + ReACp53. N = 3. (C&D) ELISA was utilized to test IL-1β (C) and IL-18 (D) concentrations in supernatant from three groups, including si-NC, si-linc00023, and si-linc00023 + ReACp53. N = 3. (E) Western blot was used to detect the expression of IL-1β and IL-18 in the groups of si-NC, si-linc00023, and si-linc00023 + ReACp53. N = 3. (F) MTS was applied to measure the following three groups’ cell proliferation abilities at 24, 48, and 72 h. N = 3. **P < 0.01, ****P < 0.0001.

    Techniques Used: Expressing, Quantitative RT-PCR, Western Blot, Enzyme-linked Immunosorbent Assay

    Related Articles

    Lysis:


    Article Title: Acetylation halts missense mutant p53 aggregation and rescues tumor suppression in non-small cell lung cancers
    Article Snippet: .. For protein solution assay, cells were treated with ReAcp53 (MedChemExpress, New Jersey, NJ) at concentrations of 5 μM for 16–20 h. Cell pellets were washed twice with PBS and lysed in RIPA lysis buffer supplemented with phosphatase inhibitors for 30 min on ice. ..

    Concentration Assay:

    Article Title: Silence of linc00023 inhibits pyroptosis and promotes cell proliferation via regulating p53.
    Article Snippet: .. For ReAcp53 treatment, ReAcp53 (Medchemexpress, Monmouth Junction, NJ, USA) with a concentration of 5 μM was added into ACHN cells and cultivated for 24 h following a previous study (Soragni et al., 2016). ..

    Virus:


    Recombinant:


    Chromatin Immunoprecipitation:


    cDNA Synthesis:




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    Fig. 4. Silence of linc00023 inhibited pyroptosis and promoted cell proliferation via regulating p53 expression in ACHN cells. (A) qRT-PCR was applied to monitor p53 and caspase-1 levels in three groups, including si-NC, si-linc00023, and si-linc00023 + <t>ReACp53.</t> N = 3. (B) Western blot was applied to evaluate the expression of caspase-1, GSDMD, NLRP3 and p53 in three groups, including si-NC, si-linc00023, and si-linc00023 + ReACp53. N = 3. (C&D) ELISA was utilized to test IL-1β (C) and IL-18 (D) concentrations in supernatant from three groups, including si-NC, si-linc00023, and si-linc00023 + ReACp53. N = 3. (E) Western blot was used to detect the expression of IL-1β and IL-18 in the groups of si-NC, si-linc00023, and si-linc00023 + ReACp53. N = 3. (F) MTS was applied to measure the following three groups’ cell proliferation abilities at 24, 48, and 72 h. N = 3. **P < 0.01, ****P < 0.0001.
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    Fig. 4. Silence of linc00023 inhibited pyroptosis and promoted cell proliferation via regulating p53 expression in ACHN cells. (A) qRT-PCR was applied to monitor p53 and caspase-1 levels in three groups, including si-NC, si-linc00023, and si-linc00023 + <t>ReACp53.</t> N = 3. (B) Western blot was applied to evaluate the expression of caspase-1, GSDMD, NLRP3 and p53 in three groups, including si-NC, si-linc00023, and si-linc00023 + ReACp53. N = 3. (C&D) ELISA was utilized to test IL-1β (C) and IL-18 (D) concentrations in supernatant from three groups, including si-NC, si-linc00023, and si-linc00023 + ReACp53. N = 3. (E) Western blot was used to detect the expression of IL-1β and IL-18 in the groups of si-NC, si-linc00023, and si-linc00023 + ReACp53. N = 3. (F) MTS was applied to measure the following three groups’ cell proliferation abilities at 24, 48, and 72 h. N = 3. **P < 0.01, ****P < 0.0001.
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    Fig. 4. Silence of linc00023 inhibited pyroptosis and promoted cell proliferation via regulating p53 expression in ACHN cells. (A) qRT-PCR was applied to monitor p53 and caspase-1 levels in three groups, including si-NC, si-linc00023, and si-linc00023 + <t>ReACp53.</t> N = 3. (B) Western blot was applied to evaluate the expression of caspase-1, GSDMD, NLRP3 and p53 in three groups, including si-NC, si-linc00023, and si-linc00023 + ReACp53. N = 3. (C&D) ELISA was utilized to test IL-1β (C) and IL-18 (D) concentrations in supernatant from three groups, including si-NC, si-linc00023, and si-linc00023 + ReACp53. N = 3. (E) Western blot was used to detect the expression of IL-1β and IL-18 in the groups of si-NC, si-linc00023, and si-linc00023 + ReACp53. N = 3. (F) MTS was applied to measure the following three groups’ cell proliferation abilities at 24, 48, and 72 h. N = 3. **P < 0.01, ****P < 0.0001.
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    Fig. 4. Silence of linc00023 inhibited pyroptosis and promoted cell proliferation via regulating p53 expression in ACHN cells. (A) qRT-PCR was applied to monitor p53 and caspase-1 levels in three groups, including si-NC, si-linc00023, and si-linc00023 + ReACp53. N = 3. (B) Western blot was applied to evaluate the expression of caspase-1, GSDMD, NLRP3 and p53 in three groups, including si-NC, si-linc00023, and si-linc00023 + ReACp53. N = 3. (C&D) ELISA was utilized to test IL-1β (C) and IL-18 (D) concentrations in supernatant from three groups, including si-NC, si-linc00023, and si-linc00023 + ReACp53. N = 3. (E) Western blot was used to detect the expression of IL-1β and IL-18 in the groups of si-NC, si-linc00023, and si-linc00023 + ReACp53. N = 3. (F) MTS was applied to measure the following three groups’ cell proliferation abilities at 24, 48, and 72 h. N = 3. **P < 0.01, ****P < 0.0001.

    Journal: Gene

    Article Title: Silence of linc00023 inhibits pyroptosis and promotes cell proliferation via regulating p53.

    doi: 10.1016/j.gene.2023.147628

    Figure Lengend Snippet: Fig. 4. Silence of linc00023 inhibited pyroptosis and promoted cell proliferation via regulating p53 expression in ACHN cells. (A) qRT-PCR was applied to monitor p53 and caspase-1 levels in three groups, including si-NC, si-linc00023, and si-linc00023 + ReACp53. N = 3. (B) Western blot was applied to evaluate the expression of caspase-1, GSDMD, NLRP3 and p53 in three groups, including si-NC, si-linc00023, and si-linc00023 + ReACp53. N = 3. (C&D) ELISA was utilized to test IL-1β (C) and IL-18 (D) concentrations in supernatant from three groups, including si-NC, si-linc00023, and si-linc00023 + ReACp53. N = 3. (E) Western blot was used to detect the expression of IL-1β and IL-18 in the groups of si-NC, si-linc00023, and si-linc00023 + ReACp53. N = 3. (F) MTS was applied to measure the following three groups’ cell proliferation abilities at 24, 48, and 72 h. N = 3. **P < 0.01, ****P < 0.0001.

    Article Snippet: For ReAcp53 treatment, ReAcp53 (Medchemexpress, Monmouth Junction, NJ, USA) with a concentration of 5 μM was added into ACHN cells and cultivated for 24 h following a previous study (Soragni et al., 2016).

    Techniques: Expressing, Quantitative RT-PCR, Western Blot, Enzyme-linked Immunosorbent Assay

    Journal: iScience

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    doi: 10.1016/j.isci.2023.107003

    Figure Lengend Snippet:

    Article Snippet: ReAcp53 , MedChemExpress , Cat# HY-P0121.

    Techniques: Virus, Recombinant, Chromatin Immunoprecipitation, Sequencing, Software