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angiotensin ii  (MedChemExpress)


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    MedChemExpress angiotensin ii
    Hopeaphenol alleviates Ang II-induced cardiomyocyte hypertrophy Via AMPK/SIRT1 activation. ( A , B ) CCK-8 assay for non-toxic ranges of hopeaphenol (0–50 μM) and the AMPK inhibitor CC (0–50 μM) (24 h). ( C ) Cells modeling flowchart. ( D – G ) Western blot analysis of p-AMPK/AMPK ( E ), SIRT1 ( F ) and BNP ( G ) in HL-1 cells with densitometric analysis. ( H – K ) Immunofluorescence of p-AMPK ( H ) and SIRT1 ( J ) with DAPI (blue) (scale bar, 25 μm); quantification of fluorescence intensity ( I , K ). ( L – M ) Phalloidin staining (green) of F-actin with DAPI (blue) ( L ) and quantification of cell cross-sectional area ( M ) (scale bar, 25 μm). Cells were pretreated with HP (1, 5, 10 μM) for 2 h or CC (10 μM) for 1 h, followed by stimulation with <t>Ang</t> <t>II</t> (1 μM) for 24 h. Exact p -values for all post hoc comparisons are indicated on the graphs. Data are presented as mean ± SEM ( n = 6). ns, not significant; HP, Hopeaphenol.
    Angiotensin Ii, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hy-p0108/Angiotensin+II+5-valine/pmc12472553-38-4-18
    Average 93 stars, based on 2 article reviews
    angiotensin ii - by Bioz Stars, 2026-10
    93/100 stars

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    1) Product Images from "Unlocking Hopeaphenol: A Potent Ally Against Cardiac Hypertrophy via AMPK Activation"

    Article Title: Unlocking Hopeaphenol: A Potent Ally Against Cardiac Hypertrophy via AMPK Activation

    Journal: Nutrients

    doi: 10.3390/nu17183025

    Hopeaphenol alleviates Ang II-induced cardiomyocyte hypertrophy Via AMPK/SIRT1 activation. ( A , B ) CCK-8 assay for non-toxic ranges of hopeaphenol (0–50 μM) and the AMPK inhibitor CC (0–50 μM) (24 h). ( C ) Cells modeling flowchart. ( D – G ) Western blot analysis of p-AMPK/AMPK ( E ), SIRT1 ( F ) and BNP ( G ) in HL-1 cells with densitometric analysis. ( H – K ) Immunofluorescence of p-AMPK ( H ) and SIRT1 ( J ) with DAPI (blue) (scale bar, 25 μm); quantification of fluorescence intensity ( I , K ). ( L – M ) Phalloidin staining (green) of F-actin with DAPI (blue) ( L ) and quantification of cell cross-sectional area ( M ) (scale bar, 25 μm). Cells were pretreated with HP (1, 5, 10 μM) for 2 h or CC (10 μM) for 1 h, followed by stimulation with Ang II (1 μM) for 24 h. Exact p -values for all post hoc comparisons are indicated on the graphs. Data are presented as mean ± SEM ( n = 6). ns, not significant; HP, Hopeaphenol.
    Figure Legend Snippet: Hopeaphenol alleviates Ang II-induced cardiomyocyte hypertrophy Via AMPK/SIRT1 activation. ( A , B ) CCK-8 assay for non-toxic ranges of hopeaphenol (0–50 μM) and the AMPK inhibitor CC (0–50 μM) (24 h). ( C ) Cells modeling flowchart. ( D – G ) Western blot analysis of p-AMPK/AMPK ( E ), SIRT1 ( F ) and BNP ( G ) in HL-1 cells with densitometric analysis. ( H – K ) Immunofluorescence of p-AMPK ( H ) and SIRT1 ( J ) with DAPI (blue) (scale bar, 25 μm); quantification of fluorescence intensity ( I , K ). ( L – M ) Phalloidin staining (green) of F-actin with DAPI (blue) ( L ) and quantification of cell cross-sectional area ( M ) (scale bar, 25 μm). Cells were pretreated with HP (1, 5, 10 μM) for 2 h or CC (10 μM) for 1 h, followed by stimulation with Ang II (1 μM) for 24 h. Exact p -values for all post hoc comparisons are indicated on the graphs. Data are presented as mean ± SEM ( n = 6). ns, not significant; HP, Hopeaphenol.

    Techniques Used: Activation Assay, CCK-8 Assay, Western Blot, Immunofluorescence, Fluorescence, Staining

    Hopeaphenol reduces mitochondrial ROS and enhances membrane potential in Ang II-treated HL-1 cardiomyocytes. ( A , B ) Mitochondrial ROS detection (DCFH-DA, green) ( A ) and quantification of fluorescence intensity ( B ) (scale bar = 25 μm). ( C , D ) JC-1 staining (red: J-aggregates, green: J-monomers) ( C ) and red/green fluorescence ratio (ΔΨm) ( D ) (scale bar = 25 μm). Cells were pretreated as in . Exact p -values for all post hoc comparisons are indicated on the graphs. Data are presented as mean ± SEM ( n = 6). ns, not significant; HP, Hopeaphenol.
    Figure Legend Snippet: Hopeaphenol reduces mitochondrial ROS and enhances membrane potential in Ang II-treated HL-1 cardiomyocytes. ( A , B ) Mitochondrial ROS detection (DCFH-DA, green) ( A ) and quantification of fluorescence intensity ( B ) (scale bar = 25 μm). ( C , D ) JC-1 staining (red: J-aggregates, green: J-monomers) ( C ) and red/green fluorescence ratio (ΔΨm) ( D ) (scale bar = 25 μm). Cells were pretreated as in . Exact p -values for all post hoc comparisons are indicated on the graphs. Data are presented as mean ± SEM ( n = 6). ns, not significant; HP, Hopeaphenol.

    Techniques Used: Membrane, Fluorescence, Staining

    AMPK inhibition by Compound C abolishes Hopeaphenol-mediated protection against Ang II-induced hypertrophy and apoptosis in HL-1 cardiomyocytes. ( A – E ) Western blot analysis of PGC-1α, SIRT1, β-MHC, and BNP ( A ) and their quantification (( B – E ), normalized to β-actin). ( F – H ) qPCR analysis of SIRT1, BNP, and β-MHC mRNA. ( I – K ) Western blot analysis of Bax, Bcl-2, and cleaved caspase-3 ( I ), and quantification of the Bax/Bcl-2 ratio ( J ) and cleaved caspase-3 ( K ). Cells were pretreated as in . Exact p values for all post hoc comparisons are indicated on the graphs. Data are presented as mean ± SEM ( n = 3 for panels ( I – K ); n = 6 for others). ns, not significant; c-Casp3, cleaved caspase-3; HP, Hopeaphenol.
    Figure Legend Snippet: AMPK inhibition by Compound C abolishes Hopeaphenol-mediated protection against Ang II-induced hypertrophy and apoptosis in HL-1 cardiomyocytes. ( A – E ) Western blot analysis of PGC-1α, SIRT1, β-MHC, and BNP ( A ) and their quantification (( B – E ), normalized to β-actin). ( F – H ) qPCR analysis of SIRT1, BNP, and β-MHC mRNA. ( I – K ) Western blot analysis of Bax, Bcl-2, and cleaved caspase-3 ( I ), and quantification of the Bax/Bcl-2 ratio ( J ) and cleaved caspase-3 ( K ). Cells were pretreated as in . Exact p values for all post hoc comparisons are indicated on the graphs. Data are presented as mean ± SEM ( n = 3 for panels ( I – K ); n = 6 for others). ns, not significant; c-Casp3, cleaved caspase-3; HP, Hopeaphenol.

    Techniques Used: Inhibition, Western Blot

    Schematic of hopeaphenol alleviating cardiac hypertrophy. The left panel shows the Ang II/TAC-induced pathological process (HL-1 cells, C57 mice), characterized by mitochondrial dysfunction, cardiomyocyte hypertrophy, and fibrosis. The right panel depicts that Hopeaphenol primarily activates the AMPK/SIRT1 signaling pathway to improve cardiac and mitochondrial function, and to inhibit hypertrophy, fibrosis, and apoptosis; notably, due to the potential off-target effects of CC (Compound C), the involvement of other pathways in these processes cannot be completely ruled out. Meanwhile, CC (Compound C) blocks the activity of AMPK, which in turn abrogates the aforementioned beneficial effects of Hopeaphenol—this observation strongly supports the role of AMPK in mediating Hopeaphenol’s cardioprotection (by Figdraw). CC, Compound C.
    Figure Legend Snippet: Schematic of hopeaphenol alleviating cardiac hypertrophy. The left panel shows the Ang II/TAC-induced pathological process (HL-1 cells, C57 mice), characterized by mitochondrial dysfunction, cardiomyocyte hypertrophy, and fibrosis. The right panel depicts that Hopeaphenol primarily activates the AMPK/SIRT1 signaling pathway to improve cardiac and mitochondrial function, and to inhibit hypertrophy, fibrosis, and apoptosis; notably, due to the potential off-target effects of CC (Compound C), the involvement of other pathways in these processes cannot be completely ruled out. Meanwhile, CC (Compound C) blocks the activity of AMPK, which in turn abrogates the aforementioned beneficial effects of Hopeaphenol—this observation strongly supports the role of AMPK in mediating Hopeaphenol’s cardioprotection (by Figdraw). CC, Compound C.

    Techniques Used: Activity Assay

    Related Articles

    Activation Assay:

    Article Title: Unlocking Hopeaphenol: A Potent Ally Against Cardiac Hypertrophy via AMPK Activation
    Article Snippet: Hopeaphenol (≥98%, #388582-37-4), was purchased from Sigma-Aldrich (St. Louis, MO, USA).Hopeaphenol (≥98%, #388582-37-4), was purchased from Sigma-Aldrich (St. Louis, MO, USA).. Compound C (#S7840) and Angiotensin II (Ang II, #HY-P0108) were obtained from Selleck Chemicals (Houston, TX, USA) and MedChemExpress (MCE, Monmouth Jct., NJ, USA), respectively.. The related antibodies used in this study included anti-phospho-AMPK (pAMPK, #83924-1-RR, Proteintech Group, Rosemont, IL, USA), anti-AMPK (#AB32047, Abcam, Cambridge, UK), and anti-SIRT1 (#DF6033, Affinity Biosciences, Cincinnati, OH, USA).The related antibodies used in this study included anti-phospho-AMPK (pAMPK, #83924-1-RR, Proteintech Group, Rosemont, IL, USA), anti-AMPK (#AB32047, Abcam, Cambridge, UK), and anti-SIRT1 (#DF6033, Affinity Biosciences, Cincinnati, OH, USA).

    Article Title: Unlocking Hopeaphenol: A Potent Ally Against Cardiac Hypertrophy via AMPK Activation
    Article Snippet: 582-37-4), was purchased from Sigma-Aldrich (St. Louis, MO, USA). Compound C (#S7840) and Angiotensin II (Ang II, #HY-P0108) were obtained from Selleck Chemicals (Houston, TX, USA) and MedChemExpress (MCE, Monmouth Jct., NJ, USA), respectively. The related antibodies used in this study included anti-phospho-AMPK (pAMPK, #83924-1-RR, Proteintech Group, Rosemont, IL, USA), anti-AMPK (#AB32047, Abcam,

    CCK-8 Assay:

    Article Title: Unlocking Hopeaphenol: A Potent Ally Against Cardiac Hypertrophy via AMPK Activation
    Article Snippet: Hopeaphenol (≥98%, #388582-37-4), was purchased from Sigma-Aldrich (St. Louis, MO, USA).Hopeaphenol (≥98%, #388582-37-4), was purchased from Sigma-Aldrich (St. Louis, MO, USA).. Compound C (#S7840) and Angiotensin II (Ang II, #HY-P0108) were obtained from Selleck Chemicals (Houston, TX, USA) and MedChemExpress (MCE, Monmouth Jct., NJ, USA), respectively.. The related antibodies used in this study included anti-phospho-AMPK (pAMPK, #83924-1-RR, Proteintech Group, Rosemont, IL, USA), anti-AMPK (#AB32047, Abcam, Cambridge, UK), and anti-SIRT1 (#DF6033, Affinity Biosciences, Cincinnati, OH, USA).The related antibodies used in this study included anti-phospho-AMPK (pAMPK, #83924-1-RR, Proteintech Group, Rosemont, IL, USA), anti-AMPK (#AB32047, Abcam, Cambridge, UK), and anti-SIRT1 (#DF6033, Affinity Biosciences, Cincinnati, OH, USA).

    Article Title: Unlocking Hopeaphenol: A Potent Ally Against Cardiac Hypertrophy via AMPK Activation
    Article Snippet: 582-37-4), was purchased from Sigma-Aldrich (St. Louis, MO, USA). Compound C (#S7840) and Angiotensin II (Ang II, #HY-P0108) were obtained from Selleck Chemicals (Houston, TX, USA) and MedChemExpress (MCE, Monmouth Jct., NJ, USA), respectively. The related antibodies used in this study included anti-phospho-AMPK (pAMPK, #83924-1-RR, Proteintech Group, Rosemont, IL, USA), anti-AMPK (#AB32047, Abcam,

    Western Blot:

    Article Title: Unlocking Hopeaphenol: A Potent Ally Against Cardiac Hypertrophy via AMPK Activation
    Article Snippet: Hopeaphenol (≥98%, #388582-37-4), was purchased from Sigma-Aldrich (St. Louis, MO, USA).Hopeaphenol (≥98%, #388582-37-4), was purchased from Sigma-Aldrich (St. Louis, MO, USA).. Compound C (#S7840) and Angiotensin II (Ang II, #HY-P0108) were obtained from Selleck Chemicals (Houston, TX, USA) and MedChemExpress (MCE, Monmouth Jct., NJ, USA), respectively.. The related antibodies used in this study included anti-phospho-AMPK (pAMPK, #83924-1-RR, Proteintech Group, Rosemont, IL, USA), anti-AMPK (#AB32047, Abcam, Cambridge, UK), and anti-SIRT1 (#DF6033, Affinity Biosciences, Cincinnati, OH, USA).The related antibodies used in this study included anti-phospho-AMPK (pAMPK, #83924-1-RR, Proteintech Group, Rosemont, IL, USA), anti-AMPK (#AB32047, Abcam, Cambridge, UK), and anti-SIRT1 (#DF6033, Affinity Biosciences, Cincinnati, OH, USA).

    Article Title: Unlocking Hopeaphenol: A Potent Ally Against Cardiac Hypertrophy via AMPK Activation
    Article Snippet: 582-37-4), was purchased from Sigma-Aldrich (St. Louis, MO, USA). Compound C (#S7840) and Angiotensin II (Ang II, #HY-P0108) were obtained from Selleck Chemicals (Houston, TX, USA) and MedChemExpress (MCE, Monmouth Jct., NJ, USA), respectively. The related antibodies used in this study included anti-phospho-AMPK (pAMPK, #83924-1-RR, Proteintech Group, Rosemont, IL, USA), anti-AMPK (#AB32047, Abcam,

    Immunofluorescence:

    Article Title: Unlocking Hopeaphenol: A Potent Ally Against Cardiac Hypertrophy via AMPK Activation
    Article Snippet: Hopeaphenol (≥98%, #388582-37-4), was purchased from Sigma-Aldrich (St. Louis, MO, USA).Hopeaphenol (≥98%, #388582-37-4), was purchased from Sigma-Aldrich (St. Louis, MO, USA).. Compound C (#S7840) and Angiotensin II (Ang II, #HY-P0108) were obtained from Selleck Chemicals (Houston, TX, USA) and MedChemExpress (MCE, Monmouth Jct., NJ, USA), respectively.. The related antibodies used in this study included anti-phospho-AMPK (pAMPK, #83924-1-RR, Proteintech Group, Rosemont, IL, USA), anti-AMPK (#AB32047, Abcam, Cambridge, UK), and anti-SIRT1 (#DF6033, Affinity Biosciences, Cincinnati, OH, USA).The related antibodies used in this study included anti-phospho-AMPK (pAMPK, #83924-1-RR, Proteintech Group, Rosemont, IL, USA), anti-AMPK (#AB32047, Abcam, Cambridge, UK), and anti-SIRT1 (#DF6033, Affinity Biosciences, Cincinnati, OH, USA).

    Article Title: Unlocking Hopeaphenol: A Potent Ally Against Cardiac Hypertrophy via AMPK Activation
    Article Snippet: 582-37-4), was purchased from Sigma-Aldrich (St. Louis, MO, USA). Compound C (#S7840) and Angiotensin II (Ang II, #HY-P0108) were obtained from Selleck Chemicals (Houston, TX, USA) and MedChemExpress (MCE, Monmouth Jct., NJ, USA), respectively. The related antibodies used in this study included anti-phospho-AMPK (pAMPK, #83924-1-RR, Proteintech Group, Rosemont, IL, USA), anti-AMPK (#AB32047, Abcam,

    Fluorescence:

    Article Title: Unlocking Hopeaphenol: A Potent Ally Against Cardiac Hypertrophy via AMPK Activation
    Article Snippet: Hopeaphenol (≥98%, #388582-37-4), was purchased from Sigma-Aldrich (St. Louis, MO, USA).Hopeaphenol (≥98%, #388582-37-4), was purchased from Sigma-Aldrich (St. Louis, MO, USA).. Compound C (#S7840) and Angiotensin II (Ang II, #HY-P0108) were obtained from Selleck Chemicals (Houston, TX, USA) and MedChemExpress (MCE, Monmouth Jct., NJ, USA), respectively.. The related antibodies used in this study included anti-phospho-AMPK (pAMPK, #83924-1-RR, Proteintech Group, Rosemont, IL, USA), anti-AMPK (#AB32047, Abcam, Cambridge, UK), and anti-SIRT1 (#DF6033, Affinity Biosciences, Cincinnati, OH, USA).The related antibodies used in this study included anti-phospho-AMPK (pAMPK, #83924-1-RR, Proteintech Group, Rosemont, IL, USA), anti-AMPK (#AB32047, Abcam, Cambridge, UK), and anti-SIRT1 (#DF6033, Affinity Biosciences, Cincinnati, OH, USA).

    Article Title: Unlocking Hopeaphenol: A Potent Ally Against Cardiac Hypertrophy via AMPK Activation
    Article Snippet: 582-37-4), was purchased from Sigma-Aldrich (St. Louis, MO, USA). Compound C (#S7840) and Angiotensin II (Ang II, #HY-P0108) were obtained from Selleck Chemicals (Houston, TX, USA) and MedChemExpress (MCE, Monmouth Jct., NJ, USA), respectively. The related antibodies used in this study included anti-phospho-AMPK (pAMPK, #83924-1-RR, Proteintech Group, Rosemont, IL, USA), anti-AMPK (#AB32047, Abcam,

    Staining:

    Article Title: Unlocking Hopeaphenol: A Potent Ally Against Cardiac Hypertrophy via AMPK Activation
    Article Snippet: Hopeaphenol (≥98%, #388582-37-4), was purchased from Sigma-Aldrich (St. Louis, MO, USA).Hopeaphenol (≥98%, #388582-37-4), was purchased from Sigma-Aldrich (St. Louis, MO, USA).. Compound C (#S7840) and Angiotensin II (Ang II, #HY-P0108) were obtained from Selleck Chemicals (Houston, TX, USA) and MedChemExpress (MCE, Monmouth Jct., NJ, USA), respectively.. The related antibodies used in this study included anti-phospho-AMPK (pAMPK, #83924-1-RR, Proteintech Group, Rosemont, IL, USA), anti-AMPK (#AB32047, Abcam, Cambridge, UK), and anti-SIRT1 (#DF6033, Affinity Biosciences, Cincinnati, OH, USA).The related antibodies used in this study included anti-phospho-AMPK (pAMPK, #83924-1-RR, Proteintech Group, Rosemont, IL, USA), anti-AMPK (#AB32047, Abcam, Cambridge, UK), and anti-SIRT1 (#DF6033, Affinity Biosciences, Cincinnati, OH, USA).

    Article Title: Unlocking Hopeaphenol: A Potent Ally Against Cardiac Hypertrophy via AMPK Activation
    Article Snippet: 582-37-4), was purchased from Sigma-Aldrich (St. Louis, MO, USA). Compound C (#S7840) and Angiotensin II (Ang II, #HY-P0108) were obtained from Selleck Chemicals (Houston, TX, USA) and MedChemExpress (MCE, Monmouth Jct., NJ, USA), respectively. The related antibodies used in this study included anti-phospho-AMPK (pAMPK, #83924-1-RR, Proteintech Group, Rosemont, IL, USA), anti-AMPK (#AB32047, Abcam,

    Membrane:

    Article Title: Unlocking Hopeaphenol: A Potent Ally Against Cardiac Hypertrophy via AMPK Activation
    Article Snippet: Hopeaphenol (≥98%, #388582-37-4), was purchased from Sigma-Aldrich (St. Louis, MO, USA).Hopeaphenol (≥98%, #388582-37-4), was purchased from Sigma-Aldrich (St. Louis, MO, USA).. Compound C (#S7840) and Angiotensin II (Ang II, #HY-P0108) were obtained from Selleck Chemicals (Houston, TX, USA) and MedChemExpress (MCE, Monmouth Jct., NJ, USA), respectively.. The related antibodies used in this study included anti-phospho-AMPK (pAMPK, #83924-1-RR, Proteintech Group, Rosemont, IL, USA), anti-AMPK (#AB32047, Abcam, Cambridge, UK), and anti-SIRT1 (#DF6033, Affinity Biosciences, Cincinnati, OH, USA).The related antibodies used in this study included anti-phospho-AMPK (pAMPK, #83924-1-RR, Proteintech Group, Rosemont, IL, USA), anti-AMPK (#AB32047, Abcam, Cambridge, UK), and anti-SIRT1 (#DF6033, Affinity Biosciences, Cincinnati, OH, USA).

    Article Title: Unlocking Hopeaphenol: A Potent Ally Against Cardiac Hypertrophy via AMPK Activation
    Article Snippet: 582-37-4), was purchased from Sigma-Aldrich (St. Louis, MO, USA). Compound C (#S7840) and Angiotensin II (Ang II, #HY-P0108) were obtained from Selleck Chemicals (Houston, TX, USA) and MedChemExpress (MCE, Monmouth Jct., NJ, USA), respectively. The related antibodies used in this study included anti-phospho-AMPK (pAMPK, #83924-1-RR, Proteintech Group, Rosemont, IL, USA), anti-AMPK (#AB32047, Abcam,

    Inhibition:

    Article Title: Unlocking Hopeaphenol: A Potent Ally Against Cardiac Hypertrophy via AMPK Activation
    Article Snippet: Hopeaphenol (≥98%, #388582-37-4), was purchased from Sigma-Aldrich (St. Louis, MO, USA).Hopeaphenol (≥98%, #388582-37-4), was purchased from Sigma-Aldrich (St. Louis, MO, USA).. Compound C (#S7840) and Angiotensin II (Ang II, #HY-P0108) were obtained from Selleck Chemicals (Houston, TX, USA) and MedChemExpress (MCE, Monmouth Jct., NJ, USA), respectively.. The related antibodies used in this study included anti-phospho-AMPK (pAMPK, #83924-1-RR, Proteintech Group, Rosemont, IL, USA), anti-AMPK (#AB32047, Abcam, Cambridge, UK), and anti-SIRT1 (#DF6033, Affinity Biosciences, Cincinnati, OH, USA).The related antibodies used in this study included anti-phospho-AMPK (pAMPK, #83924-1-RR, Proteintech Group, Rosemont, IL, USA), anti-AMPK (#AB32047, Abcam, Cambridge, UK), and anti-SIRT1 (#DF6033, Affinity Biosciences, Cincinnati, OH, USA).

    Article Title: Unlocking Hopeaphenol: A Potent Ally Against Cardiac Hypertrophy via AMPK Activation
    Article Snippet: 582-37-4), was purchased from Sigma-Aldrich (St. Louis, MO, USA). Compound C (#S7840) and Angiotensin II (Ang II, #HY-P0108) were obtained from Selleck Chemicals (Houston, TX, USA) and MedChemExpress (MCE, Monmouth Jct., NJ, USA), respectively. The related antibodies used in this study included anti-phospho-AMPK (pAMPK, #83924-1-RR, Proteintech Group, Rosemont, IL, USA), anti-AMPK (#AB32047, Abcam,

    Activity Assay:

    Article Title: Unlocking Hopeaphenol: A Potent Ally Against Cardiac Hypertrophy via AMPK Activation
    Article Snippet: Hopeaphenol (≥98%, #388582-37-4), was purchased from Sigma-Aldrich (St. Louis, MO, USA).Hopeaphenol (≥98%, #388582-37-4), was purchased from Sigma-Aldrich (St. Louis, MO, USA).. Compound C (#S7840) and Angiotensin II (Ang II, #HY-P0108) were obtained from Selleck Chemicals (Houston, TX, USA) and MedChemExpress (MCE, Monmouth Jct., NJ, USA), respectively.. The related antibodies used in this study included anti-phospho-AMPK (pAMPK, #83924-1-RR, Proteintech Group, Rosemont, IL, USA), anti-AMPK (#AB32047, Abcam, Cambridge, UK), and anti-SIRT1 (#DF6033, Affinity Biosciences, Cincinnati, OH, USA).The related antibodies used in this study included anti-phospho-AMPK (pAMPK, #83924-1-RR, Proteintech Group, Rosemont, IL, USA), anti-AMPK (#AB32047, Abcam, Cambridge, UK), and anti-SIRT1 (#DF6033, Affinity Biosciences, Cincinnati, OH, USA).

    Article Title: Unlocking Hopeaphenol: A Potent Ally Against Cardiac Hypertrophy via AMPK Activation
    Article Snippet: 582-37-4), was purchased from Sigma-Aldrich (St. Louis, MO, USA). Compound C (#S7840) and Angiotensin II (Ang II, #HY-P0108) were obtained from Selleck Chemicals (Houston, TX, USA) and MedChemExpress (MCE, Monmouth Jct., NJ, USA), respectively. The related antibodies used in this study included anti-phospho-AMPK (pAMPK, #83924-1-RR, Proteintech Group, Rosemont, IL, USA), anti-AMPK (#AB32047, Abcam,



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    Hopeaphenol alleviates Ang II-induced cardiomyocyte hypertrophy Via AMPK/SIRT1 activation. ( A , B ) CCK-8 assay for non-toxic ranges of hopeaphenol (0–50 μM) and the AMPK inhibitor CC (0–50 μM) (24 h). ( C ) Cells modeling flowchart. ( D – G ) Western blot analysis of p-AMPK/AMPK ( E ), SIRT1 ( F ) and BNP ( G ) in HL-1 cells with densitometric analysis. ( H – K ) Immunofluorescence of p-AMPK ( H ) and SIRT1 ( J ) with DAPI (blue) (scale bar, 25 μm); quantification of fluorescence intensity ( I , K ). ( L – M ) Phalloidin staining (green) of F-actin with DAPI (blue) ( L ) and quantification of cell cross-sectional area ( M ) (scale bar, 25 μm). Cells were pretreated with HP (1, 5, 10 μM) for 2 h or CC (10 μM) for 1 h, followed by stimulation with <t>Ang</t> <t>II</t> (1 μM) for 24 h. Exact p -values for all post hoc comparisons are indicated on the graphs. Data are presented as mean ± SEM ( n = 6). ns, not significant; HP, Hopeaphenol.
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    Hopeaphenol alleviates Ang II-induced cardiomyocyte hypertrophy Via AMPK/SIRT1 activation. ( A , B ) CCK-8 assay for non-toxic ranges of hopeaphenol (0–50 μM) and the AMPK inhibitor CC (0–50 μM) (24 h). ( C ) Cells modeling flowchart. ( D – G ) Western blot analysis of p-AMPK/AMPK ( E ), SIRT1 ( F ) and BNP ( G ) in HL-1 cells with densitometric analysis. ( H – K ) Immunofluorescence of p-AMPK ( H ) and SIRT1 ( J ) with DAPI (blue) (scale bar, 25 μm); quantification of fluorescence intensity ( I , K ). ( L – M ) Phalloidin staining (green) of F-actin with DAPI (blue) ( L ) and quantification of cell cross-sectional area ( M ) (scale bar, 25 μm). Cells were pretreated with HP (1, 5, 10 μM) for 2 h or CC (10 μM) for 1 h, followed by stimulation with Ang II (1 μM) for 24 h. Exact p -values for all post hoc comparisons are indicated on the graphs. Data are presented as mean ± SEM ( n = 6). ns, not significant; HP, Hopeaphenol.

    Journal: Nutrients

    Article Title: Unlocking Hopeaphenol: A Potent Ally Against Cardiac Hypertrophy via AMPK Activation

    doi: 10.3390/nu17183025

    Figure Lengend Snippet: Hopeaphenol alleviates Ang II-induced cardiomyocyte hypertrophy Via AMPK/SIRT1 activation. ( A , B ) CCK-8 assay for non-toxic ranges of hopeaphenol (0–50 μM) and the AMPK inhibitor CC (0–50 μM) (24 h). ( C ) Cells modeling flowchart. ( D – G ) Western blot analysis of p-AMPK/AMPK ( E ), SIRT1 ( F ) and BNP ( G ) in HL-1 cells with densitometric analysis. ( H – K ) Immunofluorescence of p-AMPK ( H ) and SIRT1 ( J ) with DAPI (blue) (scale bar, 25 μm); quantification of fluorescence intensity ( I , K ). ( L – M ) Phalloidin staining (green) of F-actin with DAPI (blue) ( L ) and quantification of cell cross-sectional area ( M ) (scale bar, 25 μm). Cells were pretreated with HP (1, 5, 10 μM) for 2 h or CC (10 μM) for 1 h, followed by stimulation with Ang II (1 μM) for 24 h. Exact p -values for all post hoc comparisons are indicated on the graphs. Data are presented as mean ± SEM ( n = 6). ns, not significant; HP, Hopeaphenol.

    Article Snippet: Compound C (#S7840) and Angiotensin II (Ang II, #HY-P0108) were obtained from Selleck Chemicals (Houston, TX, USA) and MedChemExpress (MCE, Monmouth Jct., NJ, USA), respectively.

    Techniques: Activation Assay, CCK-8 Assay, Western Blot, Immunofluorescence, Fluorescence, Staining

    Hopeaphenol reduces mitochondrial ROS and enhances membrane potential in Ang II-treated HL-1 cardiomyocytes. ( A , B ) Mitochondrial ROS detection (DCFH-DA, green) ( A ) and quantification of fluorescence intensity ( B ) (scale bar = 25 μm). ( C , D ) JC-1 staining (red: J-aggregates, green: J-monomers) ( C ) and red/green fluorescence ratio (ΔΨm) ( D ) (scale bar = 25 μm). Cells were pretreated as in . Exact p -values for all post hoc comparisons are indicated on the graphs. Data are presented as mean ± SEM ( n = 6). ns, not significant; HP, Hopeaphenol.

    Journal: Nutrients

    Article Title: Unlocking Hopeaphenol: A Potent Ally Against Cardiac Hypertrophy via AMPK Activation

    doi: 10.3390/nu17183025

    Figure Lengend Snippet: Hopeaphenol reduces mitochondrial ROS and enhances membrane potential in Ang II-treated HL-1 cardiomyocytes. ( A , B ) Mitochondrial ROS detection (DCFH-DA, green) ( A ) and quantification of fluorescence intensity ( B ) (scale bar = 25 μm). ( C , D ) JC-1 staining (red: J-aggregates, green: J-monomers) ( C ) and red/green fluorescence ratio (ΔΨm) ( D ) (scale bar = 25 μm). Cells were pretreated as in . Exact p -values for all post hoc comparisons are indicated on the graphs. Data are presented as mean ± SEM ( n = 6). ns, not significant; HP, Hopeaphenol.

    Article Snippet: Compound C (#S7840) and Angiotensin II (Ang II, #HY-P0108) were obtained from Selleck Chemicals (Houston, TX, USA) and MedChemExpress (MCE, Monmouth Jct., NJ, USA), respectively.

    Techniques: Membrane, Fluorescence, Staining

    AMPK inhibition by Compound C abolishes Hopeaphenol-mediated protection against Ang II-induced hypertrophy and apoptosis in HL-1 cardiomyocytes. ( A – E ) Western blot analysis of PGC-1α, SIRT1, β-MHC, and BNP ( A ) and their quantification (( B – E ), normalized to β-actin). ( F – H ) qPCR analysis of SIRT1, BNP, and β-MHC mRNA. ( I – K ) Western blot analysis of Bax, Bcl-2, and cleaved caspase-3 ( I ), and quantification of the Bax/Bcl-2 ratio ( J ) and cleaved caspase-3 ( K ). Cells were pretreated as in . Exact p values for all post hoc comparisons are indicated on the graphs. Data are presented as mean ± SEM ( n = 3 for panels ( I – K ); n = 6 for others). ns, not significant; c-Casp3, cleaved caspase-3; HP, Hopeaphenol.

    Journal: Nutrients

    Article Title: Unlocking Hopeaphenol: A Potent Ally Against Cardiac Hypertrophy via AMPK Activation

    doi: 10.3390/nu17183025

    Figure Lengend Snippet: AMPK inhibition by Compound C abolishes Hopeaphenol-mediated protection against Ang II-induced hypertrophy and apoptosis in HL-1 cardiomyocytes. ( A – E ) Western blot analysis of PGC-1α, SIRT1, β-MHC, and BNP ( A ) and their quantification (( B – E ), normalized to β-actin). ( F – H ) qPCR analysis of SIRT1, BNP, and β-MHC mRNA. ( I – K ) Western blot analysis of Bax, Bcl-2, and cleaved caspase-3 ( I ), and quantification of the Bax/Bcl-2 ratio ( J ) and cleaved caspase-3 ( K ). Cells were pretreated as in . Exact p values for all post hoc comparisons are indicated on the graphs. Data are presented as mean ± SEM ( n = 3 for panels ( I – K ); n = 6 for others). ns, not significant; c-Casp3, cleaved caspase-3; HP, Hopeaphenol.

    Article Snippet: Compound C (#S7840) and Angiotensin II (Ang II, #HY-P0108) were obtained from Selleck Chemicals (Houston, TX, USA) and MedChemExpress (MCE, Monmouth Jct., NJ, USA), respectively.

    Techniques: Inhibition, Western Blot

    Schematic of hopeaphenol alleviating cardiac hypertrophy. The left panel shows the Ang II/TAC-induced pathological process (HL-1 cells, C57 mice), characterized by mitochondrial dysfunction, cardiomyocyte hypertrophy, and fibrosis. The right panel depicts that Hopeaphenol primarily activates the AMPK/SIRT1 signaling pathway to improve cardiac and mitochondrial function, and to inhibit hypertrophy, fibrosis, and apoptosis; notably, due to the potential off-target effects of CC (Compound C), the involvement of other pathways in these processes cannot be completely ruled out. Meanwhile, CC (Compound C) blocks the activity of AMPK, which in turn abrogates the aforementioned beneficial effects of Hopeaphenol—this observation strongly supports the role of AMPK in mediating Hopeaphenol’s cardioprotection (by Figdraw). CC, Compound C.

    Journal: Nutrients

    Article Title: Unlocking Hopeaphenol: A Potent Ally Against Cardiac Hypertrophy via AMPK Activation

    doi: 10.3390/nu17183025

    Figure Lengend Snippet: Schematic of hopeaphenol alleviating cardiac hypertrophy. The left panel shows the Ang II/TAC-induced pathological process (HL-1 cells, C57 mice), characterized by mitochondrial dysfunction, cardiomyocyte hypertrophy, and fibrosis. The right panel depicts that Hopeaphenol primarily activates the AMPK/SIRT1 signaling pathway to improve cardiac and mitochondrial function, and to inhibit hypertrophy, fibrosis, and apoptosis; notably, due to the potential off-target effects of CC (Compound C), the involvement of other pathways in these processes cannot be completely ruled out. Meanwhile, CC (Compound C) blocks the activity of AMPK, which in turn abrogates the aforementioned beneficial effects of Hopeaphenol—this observation strongly supports the role of AMPK in mediating Hopeaphenol’s cardioprotection (by Figdraw). CC, Compound C.

    Article Snippet: Compound C (#S7840) and Angiotensin II (Ang II, #HY-P0108) were obtained from Selleck Chemicals (Houston, TX, USA) and MedChemExpress (MCE, Monmouth Jct., NJ, USA), respectively.

    Techniques: Activity Assay