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cck  (MedChemExpress)


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    Structured Review

    MedChemExpress cck
    Therapeutic efficacy of in Vitro treatment . a) Detection of live/dead cells via the double-staining assay with acridine orange (AO) and ethylpyridine bromide (EB) in the PBS, Exo-CTX, Exo@siRNA-CTX, Exo@3BP-CTX or ERBC nanovector groups; scale bar:100 μm. b) Analysis of the cytotoxicity of SW480 cells measured with <t>the</t> <t>CCK-8</t> kit after treatment with PBS, Exo-CTX, Exo@siRNA-CTX, Exo@3BP-CTX, or ERBC, with or without free CTX (250 μg ml −1 ). The date are expressed as the mean ± SD (n = 5). c) Western blot analysis of Caspase 3 expression after SW480 cells treated with different treatments for 48 h. d) Statistical results of c). Data are presented as mean ± SD. n = 3. e) The FCM analysis of apoptotic of SW480 cells subjected to different treatments for 48 h via Annexin V-FITC and PI assays. The sample size was calculated as 10,000 cells using a flow cytometer (events) in each sample. n = 3. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Cck, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hy-p0093/Sincalide/pmc12022660-62-5-9
    Average 94 stars, based on 30 article reviews
    cck - by Bioz Stars, 2026-10
    94/100 stars

    Images

    1) Product Images from "An exosome-based nanoplatform for siRNA delivery combined with starvation therapy promotes tumor cell death through autophagy, overcoming refractory KRAS-mutated tumors and restoring cetuximab chemosensitivity"

    Article Title: An exosome-based nanoplatform for siRNA delivery combined with starvation therapy promotes tumor cell death through autophagy, overcoming refractory KRAS-mutated tumors and restoring cetuximab chemosensitivity

    Journal: Materials Today Bio

    doi: 10.1016/j.mtbio.2025.101732

    Therapeutic efficacy of in Vitro treatment . a) Detection of live/dead cells via the double-staining assay with acridine orange (AO) and ethylpyridine bromide (EB) in the PBS, Exo-CTX, Exo@siRNA-CTX, Exo@3BP-CTX or ERBC nanovector groups; scale bar:100 μm. b) Analysis of the cytotoxicity of SW480 cells measured with the CCK-8 kit after treatment with PBS, Exo-CTX, Exo@siRNA-CTX, Exo@3BP-CTX, or ERBC, with or without free CTX (250 μg ml −1 ). The date are expressed as the mean ± SD (n = 5). c) Western blot analysis of Caspase 3 expression after SW480 cells treated with different treatments for 48 h. d) Statistical results of c). Data are presented as mean ± SD. n = 3. e) The FCM analysis of apoptotic of SW480 cells subjected to different treatments for 48 h via Annexin V-FITC and PI assays. The sample size was calculated as 10,000 cells using a flow cytometer (events) in each sample. n = 3. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
    Figure Legend Snippet: Therapeutic efficacy of in Vitro treatment . a) Detection of live/dead cells via the double-staining assay with acridine orange (AO) and ethylpyridine bromide (EB) in the PBS, Exo-CTX, Exo@siRNA-CTX, Exo@3BP-CTX or ERBC nanovector groups; scale bar:100 μm. b) Analysis of the cytotoxicity of SW480 cells measured with the CCK-8 kit after treatment with PBS, Exo-CTX, Exo@siRNA-CTX, Exo@3BP-CTX, or ERBC, with or without free CTX (250 μg ml −1 ). The date are expressed as the mean ± SD (n = 5). c) Western blot analysis of Caspase 3 expression after SW480 cells treated with different treatments for 48 h. d) Statistical results of c). Data are presented as mean ± SD. n = 3. e) The FCM analysis of apoptotic of SW480 cells subjected to different treatments for 48 h via Annexin V-FITC and PI assays. The sample size was calculated as 10,000 cells using a flow cytometer (events) in each sample. n = 3. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Techniques Used: Drug discovery, In Vitro, Double Staining, CCK-8 Assay, Western Blot, Expressing, Flow Cytometry

    Related Articles

    other:

    Article Title: Sarcopenia promotes tumorigenesis by disrupting NOTCH-SDC2-regulated biogenesis of muscle-derived extracellular vesicles.
    Article Snippet: Following the treatment, cell viability was assessed using the Cell Counting Kit-8 (CCK-8, MedChemExpress, #HY-K0301) according to the manufacturer's instructions.

    Article Title: Targeting UBE2T by β-elemene inhibits prostate cancer stem cells and bone metastasis by blocking the TRIM28/pGSK3β/β-catenin signaling.
    Article Snippet: The effect of varying concentrations of β-elemene (HY-107,324, MCE) on the viability of PCa cells was assessed using a CCK-8 assay.

    Article Title: A spinal USP7-Bach1 positive feedback loop drives NOX4-mediated ferroptosis in neuropathic pain
    Article Snippet: Cell viability was assessed using the Cell Counting Kit-8 (CCK-8, HY-K0301, MCE, China) according to the manufacturer's instructions.

    Article Title: Insulin-like growth factor-binding protein 5 promotes prostate cancer metastasis and osteoblastic activity by inducing chemokines and activating NF-κB signaling.
    Article Snippet: AR TIC LE IN PR ES S ARTICLE IN PRESS 2.6 CCK8 assay Cell viability was evaluated using the Cell Counting Kit-8 (CCK-8, HY-K0301, MCE) according to the manufacturer’s instructions.

    Article Title: Extracellular vesicles derived from ovarian cancer cells promote tumor progression through m2 macrophage polarization and enhanced angiogenesis.
    Article Snippet: The proliferation of HUVECs co-cultured with EV-treated macrophages was assessed using the Cell Counting Kit-8 (CCK-8, MCE, USA) according to the manufacturer's instructions.

    CCK-8 Assay:

    Article Title: Role of PTK6 in the diagnosis, prognosis, and immune infiltration of pancreatic cancer based on evidence from bioinformatics analysis and experimental validation.
    Article Snippet: .. Cell proliferation was measured using the Cell Counting Kit-8 (CCK-8; MCE, USA), and statistical analysis was performed. ..

    Article Title: Mannosylated graphene oxide nanotherapeutics co-delivering docetaxel and a STING agonist reprogram myeloid cells and potentiate antitumor immunity
    Article Snippet: .. Docetaxel, sulfo-Cy5.5, the Cell Counting Kit-8 (CCK-8) and rhodamine B were purchased from MedChemExpress (Monmouth Junction, NJ, USA). .. Graphene oxide was supplied by Nanjing XFNANO Materials Tech Co., Ltd. (Nanjing, China).

    Article Title: Reprogramming tumor microenvironment of pancreatic cancer by CAF-targeted sonodynamic therapy combined with chemotherapy
    Article Snippet: .. A Cell Counting Kit-8 assay (CCK-8) (MCE, USA) was utilized to examine cellular cytotoxicity. ..



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