Extraction:Article Title: Green Approach for Rosa damascena Mill. Petal Extract: Insights into Phytochemical Composition, Anti-Aging Potential, and Stability
Article Snippet: .. Compounds RT (min) Chemical Content (mg Per g R. damascena Petal Extract) INF UAE MCE-T20 MCE-T80 MAE PEF L-ascorbic acid 2.60 3.59 ± 0.00 e 3.97 ± 0.00 d 5.50 ± 0.01 c 5.62 ± 0.01 b 9.97 ± 0.01 a 3.98 ± 0.00 d Gallic acid 3.62 7.05 ± 0.00 d 8.20 ± 0.01 c 8.77 ± 0.01 b 8.75 ± 0.01 b 72.56 ± 0.03 a 6.41 ± 0.01 e Cyanidin-3,5-O-diglucoside 5.24 14.11 ± 0.01 c 14.29 ± 0.01 c 0.00 ± 0.00 d 0.00 ± 0.00 d 82.92 ± 0.02 a 15.30 ± 0.01 b Corilagin 8.46 34.30 ± 0.01 c 34.90 ± 0.02 b 23.29 ± 0.01 e 14.68 ± 0.01 f 213.59 ± 0.01 a 25.16 ± 0.01 d Rutin 13.21 4.30 ± 0.01 d 3.81 ± 0.01 e 4.74 ± 0.01 c 5.21 ± 0.01 b 6.70 ± 0.01 a 3.62 ± 0.00 f Ellagic acid 14.74 22.97 ± 0.01 d 22.37 ± 0.01 e 23.74 ± 0.01 c 24.67 ± 0.01 b 48.49 ± 0.01 a 17.45 ± 0.01 f NOTE: R. damascena petals were extracted using various green extraction methods, including infusion (INF), ultrasonic-assisted extraction (UAE), micellar extraction using Tween® 20 (MCE-T20), micellar extraction using Tween® 80 (MCE-T80), microwave-assisted extraction (MAE), and pulsed electric field extraction (PEF). ..
Article Title: Green Approach for Rosa damascena Mill. Petal Extract: Insights into Phytochemical Composition, Anti-Aging Potential, and Stability
Article Snippet: .. Abbreviations The following abbreviations are used in this manuscript: AAAVPN N-Succinyl-Ala-Ala-Ala-p-nitroanilide ABTS 2,2-Azino-bis3-ethylbenzothiazoline-6-sulfonic acid BSA Bovine serum albumin DI Deionized DPPH 2,2-Diphenyl-1picrylhydrazyl EC1 Equivalent capacity ECM Extracellular matrix EGCG Epigallocatechin gallate FALGPA 2-Furanacryloyl-Leu-Gly-Pro-Ala HPLC High performance liquid chromatography IC50 Half-maximal inhibitory concentration INF Infusion MAE Microwave-assisted extraction MCE Micellar extraction MCE-T20 Micellar extraction using Tween® 20 MCE-T80 Micellar extraction using Tween® 80 MMP-1 Metalloproteinase-1 NLCs Nanostructured lipid carriers Antioxidants 2025, 14, 541 25 of 28 PEF Pulsed electric fields extraction SLNs Solid lipid nanoparticles TEAC Trolox equivalent antioxidant capacity TPTZ 2,4,6 Tripyridyl-s-triazine UAE Ultrasound-assisted extraction UV Ultraviolet ..
High Performance Liquid Chromatography:Article Title: Green Approach for Rosa damascena Mill. Petal Extract: Insights into Phytochemical Composition, Anti-Aging Potential, and Stability
Article Snippet: .. Abbreviations The following abbreviations are used in this manuscript: AAAVPN N-Succinyl-Ala-Ala-Ala-p-nitroanilide ABTS 2,2-Azino-bis3-ethylbenzothiazoline-6-sulfonic acid BSA Bovine serum albumin DI Deionized DPPH 2,2-Diphenyl-1picrylhydrazyl EC1 Equivalent capacity ECM Extracellular matrix EGCG Epigallocatechin gallate FALGPA 2-Furanacryloyl-Leu-Gly-Pro-Ala HPLC High performance liquid chromatography IC50 Half-maximal inhibitory concentration INF Infusion MAE Microwave-assisted extraction MCE Micellar extraction MCE-T20 Micellar extraction using Tween® 20 MCE-T80 Micellar extraction using Tween® 80 MMP-1 Metalloproteinase-1 NLCs Nanostructured lipid carriers Antioxidants 2025, 14, 541 25 of 28 PEF Pulsed electric fields extraction SLNs Solid lipid nanoparticles TEAC Trolox equivalent antioxidant capacity TPTZ 2,4,6 Tripyridyl-s-triazine UAE Ultrasound-assisted extraction UV Ultraviolet ..
Concentration Assay:Article Title: Green Approach for Rosa damascena Mill. Petal Extract: Insights into Phytochemical Composition, Anti-Aging Potential, and Stability
Article Snippet: .. Abbreviations The following abbreviations are used in this manuscript: AAAVPN N-Succinyl-Ala-Ala-Ala-p-nitroanilide ABTS 2,2-Azino-bis3-ethylbenzothiazoline-6-sulfonic acid BSA Bovine serum albumin DI Deionized DPPH 2,2-Diphenyl-1picrylhydrazyl EC1 Equivalent capacity ECM Extracellular matrix EGCG Epigallocatechin gallate FALGPA 2-Furanacryloyl-Leu-Gly-Pro-Ala HPLC High performance liquid chromatography IC50 Half-maximal inhibitory concentration INF Infusion MAE Microwave-assisted extraction MCE Micellar extraction MCE-T20 Micellar extraction using Tween® 20 MCE-T80 Micellar extraction using Tween® 80 MMP-1 Metalloproteinase-1 NLCs Nanostructured lipid carriers Antioxidants 2025, 14, 541 25 of 28 PEF Pulsed electric fields extraction SLNs Solid lipid nanoparticles TEAC Trolox equivalent antioxidant capacity TPTZ 2,4,6 Tripyridyl-s-triazine UAE Ultrasound-assisted extraction UV Ultraviolet ..
Purification:Article Title: BACH2 promotes seeding and establishment of long-lived HIV-1 reservoir in memory CD4 + T cells.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Zombie Aqua TM Fixable Viability Kit BioLegend 423102 (+)-JQ1 Selleckchem S7110 AZD5153 6-hydroxy-2-naphthoic acid Selleckchem S8344 Ultra-LEAF TM Purified anti-human CD3 Biolegend 300333 Ultra-LEAF TM Purified anti-human CD28 Biolegend 302943 Recombinant Human IL-2 BioLegend 589106 Recombinant Human IL-7 BioLegend 581904 Recombinant Human IL-12(p70) BioLegend 573004 Recombinant Human IL-15 BioLegend 570306 Recombinant Human IL-18 BioLegend 592106 Recombinant Human TNF-α BioLegend 570104 Recombinant Human SCF STEMCELL Technologies 78062 Recombinant Human FLT3 STEMCELL Technologies 78009 Recombinant Human TPO STEMCELL Technologies 78210 Phytohemagglutinin-L (PHA-L) Sigma 11249738001 Enfuvirtide (T-20) MedChemExpress HY-P0052 Raltegravir (RAL) MedChemExpress HY-10353 Recombinant Cas9 protein IDT 1081059 Random Primers Invitrogen 48190–011 ProtoScript II Reverse Transcriptase NEB M0368 RNase Inhibitor, Murine NEB M0314L Oligo(dT)12–18 Primer Invitrogen 18418012 TaqMan TM Fast Advanced Master Mix for qPCR Applied Biosystems 4444556 Lenti-X Concentrator TaKaRa 631232 Ficoll-Paque PLUS GE Healthcare 17144003 Percoll® Sigma GE17-0891-01 HEPARIN Sodium Sagent Pharmaceuticals 25021 Citrate-dextrose solution (ACD) Sigma-Aldrich C3821 RBC Lysis Buffer (10×) Biolegend 420302 Paraformaldehyde Solution, 4% in PBS Thermo Fisher Scientific J19943K2 DNAse I Sigma 11284932001 Collagenase D Sigma 11088858001 EDTA (0.5 M), pH 8.0, RNase-free Invitrogen AM9260G Puromycin (hydrochloride) Cayman Chemical 13884 Critical commercial assays MojoSort TM Human CD4 T cell Isolation Kit BioLegend 480010 Human cord blood CD34 isolation kit STEMCELL Technologies 17896 MojoSort TM Human CD8 Nanobeads BioLegend 480108 Human IFN-alpha 2 Quantikine HS ELISA Kit R&D Systems HSIFNA2 Human Total IL-18 DuoSet ELISA R&D Systems DY318-05 P3 Primary Cell 4D-Nucleofector X Kit Lonza V4XP-3032 Quick-RNA Viral Kit Zymo Research R1035 Quick-RNA Microprep Kit Zymo Research R1051 Direct-zol RNA Miniprep Kit Zymo Research R2051 CFSE Cell Division Tracker Kit BioLegend 423801 MitoProbe TM JC-1 Assay Kit Invitrogen M34152 MitoTracker TM Dyes for Mitochondria Labeling Invitrogen M22426 QIAamp DNA Mini Kit Qiagen 51304 (Continued on next page) Please cite this article in press as: Cell Reports Medicine 6, 102311, September 16, 2025 e3 Article ll OPEN ACCESS ..
Article Title: BACH2 promotes seeding and establishment of long-lived HIV-1 reservoir in memory CD4 + T cells
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Zombie AquaTM Fixable Viability Kit BioLegend 423102 (+)-JQ1 Selleckchem S7110 AZD5153 6-hydroxy-2-naphthoic acid Selleckchem S8344 Ultra-LEAFTM Purified anti-human CD3 Biolegend 300333 Ultra-LEAFTM Purified anti-human CD28 Biolegend 302943 Recombinant Human IL-2 BioLegend 589106 Recombinant Human IL-7 BioLegend 581904 Recombinant Human IL-12(p70) BioLegend 573004 Recombinant Human IL-15 BioLegend 570306 Recombinant Human IL-18 BioLegend 592106 Recombinant Human TNF-α BioLegend 570104 Recombinant Human SCF STEMCELL Technologies 78062 Recombinant Human FLT3 STEMCELL Technologies 78009 Recombinant Human TPO STEMCELL Technologies 78210 Phytohemagglutinin-L (PHA-L) Sigma 11249738001 Enfuvirtide (T-20) MedChemExpress HY-P0052 Raltegravir (RAL) MedChemExpress HY-10353 Recombinant Cas9 protein IDT 1081059 Random Primers Invitrogen 48190–011 ProtoScript II Reverse Transcriptase NEB M0368 RNase Inhibitor, Murine NEB M0314L Oligo(dT)12–18 Primer Invitrogen 18418012 TaqManTM Fast Advanced Master Mix for qPCR Applied Biosystems 4444556 Lenti-X Concentrator TaKaRa 631232 Ficoll-Paque PLUS GE Healthcare 17144003 Percoll® Sigma GE17-0891-01 HEPARIN Sodium Sagent Pharmaceuticals 25021 Citrate-dextrose solution (ACD) Sigma-Aldrich C3821 RBC Lysis Buffer (10×) Biolegend 420302 Paraformaldehyde Solution, 4% in PBS Thermo Fisher Scientific J19943K2 DNAse I Sigma 11284932001 Collagenase D Sigma 11088858001 EDTA (0.5 M), pH 8.0, RNase-free Invitrogen AM9260G Puromycin (hydrochloride) Cayman Chemical 13884 Critical commercial assays MojoSortTM Human CD4 T cell Isolation Kit BioLegend 480010 Human cord blood CD34 isolation kit STEMCELL Technologies 17896 MojoSortTM Human CD8 Nanobeads BioLegend 480108 Human IFN-alpha 2 Quantikine HS ELISA Kit R&D Systems HSIFNA2 Human Total IL-18 DuoSet ELISA R&D Systems DY318-05 P3 Primary Cell 4D-Nucleofector X Kit Lonza V4XP-3032 Quick-RNA Viral Kit Zymo Research R1035 Quick-RNA Microprep Kit Zymo Research R1051 Direct-zol RNA Miniprep Kit Zymo Research R2051 CFSE Cell Division Tracker Kit BioLegend 423801 MitoProbeTM JC-1 Assay Kit Invitrogen M34152 MitoTrackerTM Dyes for Mitochondria Labeling Invitrogen M22426 QIAamp DNA Mini Kit Qiagen 51304 (Continued on next page) Cell Reports Medicine 6, 102311, September 16, 2025 e3 Article ll OPEN ACCESS ..
Recombinant:Article Title: BACH2 promotes seeding and establishment of long-lived HIV-1 reservoir in memory CD4 + T cells.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Zombie Aqua TM Fixable Viability Kit BioLegend 423102 (+)-JQ1 Selleckchem S7110 AZD5153 6-hydroxy-2-naphthoic acid Selleckchem S8344 Ultra-LEAF TM Purified anti-human CD3 Biolegend 300333 Ultra-LEAF TM Purified anti-human CD28 Biolegend 302943 Recombinant Human IL-2 BioLegend 589106 Recombinant Human IL-7 BioLegend 581904 Recombinant Human IL-12(p70) BioLegend 573004 Recombinant Human IL-15 BioLegend 570306 Recombinant Human IL-18 BioLegend 592106 Recombinant Human TNF-α BioLegend 570104 Recombinant Human SCF STEMCELL Technologies 78062 Recombinant Human FLT3 STEMCELL Technologies 78009 Recombinant Human TPO STEMCELL Technologies 78210 Phytohemagglutinin-L (PHA-L) Sigma 11249738001 Enfuvirtide (T-20) MedChemExpress HY-P0052 Raltegravir (RAL) MedChemExpress HY-10353 Recombinant Cas9 protein IDT 1081059 Random Primers Invitrogen 48190–011 ProtoScript II Reverse Transcriptase NEB M0368 RNase Inhibitor, Murine NEB M0314L Oligo(dT)12–18 Primer Invitrogen 18418012 TaqMan TM Fast Advanced Master Mix for qPCR Applied Biosystems 4444556 Lenti-X Concentrator TaKaRa 631232 Ficoll-Paque PLUS GE Healthcare 17144003 Percoll® Sigma GE17-0891-01 HEPARIN Sodium Sagent Pharmaceuticals 25021 Citrate-dextrose solution (ACD) Sigma-Aldrich C3821 RBC Lysis Buffer (10×) Biolegend 420302 Paraformaldehyde Solution, 4% in PBS Thermo Fisher Scientific J19943K2 DNAse I Sigma 11284932001 Collagenase D Sigma 11088858001 EDTA (0.5 M), pH 8.0, RNase-free Invitrogen AM9260G Puromycin (hydrochloride) Cayman Chemical 13884 Critical commercial assays MojoSort TM Human CD4 T cell Isolation Kit BioLegend 480010 Human cord blood CD34 isolation kit STEMCELL Technologies 17896 MojoSort TM Human CD8 Nanobeads BioLegend 480108 Human IFN-alpha 2 Quantikine HS ELISA Kit R&D Systems HSIFNA2 Human Total IL-18 DuoSet ELISA R&D Systems DY318-05 P3 Primary Cell 4D-Nucleofector X Kit Lonza V4XP-3032 Quick-RNA Viral Kit Zymo Research R1035 Quick-RNA Microprep Kit Zymo Research R1051 Direct-zol RNA Miniprep Kit Zymo Research R2051 CFSE Cell Division Tracker Kit BioLegend 423801 MitoProbe TM JC-1 Assay Kit Invitrogen M34152 MitoTracker TM Dyes for Mitochondria Labeling Invitrogen M22426 QIAamp DNA Mini Kit Qiagen 51304 (Continued on next page) Please cite this article in press as: Cell Reports Medicine 6, 102311, September 16, 2025 e3 Article ll OPEN ACCESS ..
Article Title: BACH2 promotes seeding and establishment of long-lived HIV-1 reservoir in memory CD4 + T cells
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Zombie AquaTM Fixable Viability Kit BioLegend 423102 (+)-JQ1 Selleckchem S7110 AZD5153 6-hydroxy-2-naphthoic acid Selleckchem S8344 Ultra-LEAFTM Purified anti-human CD3 Biolegend 300333 Ultra-LEAFTM Purified anti-human CD28 Biolegend 302943 Recombinant Human IL-2 BioLegend 589106 Recombinant Human IL-7 BioLegend 581904 Recombinant Human IL-12(p70) BioLegend 573004 Recombinant Human IL-15 BioLegend 570306 Recombinant Human IL-18 BioLegend 592106 Recombinant Human TNF-α BioLegend 570104 Recombinant Human SCF STEMCELL Technologies 78062 Recombinant Human FLT3 STEMCELL Technologies 78009 Recombinant Human TPO STEMCELL Technologies 78210 Phytohemagglutinin-L (PHA-L) Sigma 11249738001 Enfuvirtide (T-20) MedChemExpress HY-P0052 Raltegravir (RAL) MedChemExpress HY-10353 Recombinant Cas9 protein IDT 1081059 Random Primers Invitrogen 48190–011 ProtoScript II Reverse Transcriptase NEB M0368 RNase Inhibitor, Murine NEB M0314L Oligo(dT)12–18 Primer Invitrogen 18418012 TaqManTM Fast Advanced Master Mix for qPCR Applied Biosystems 4444556 Lenti-X Concentrator TaKaRa 631232 Ficoll-Paque PLUS GE Healthcare 17144003 Percoll® Sigma GE17-0891-01 HEPARIN Sodium Sagent Pharmaceuticals 25021 Citrate-dextrose solution (ACD) Sigma-Aldrich C3821 RBC Lysis Buffer (10×) Biolegend 420302 Paraformaldehyde Solution, 4% in PBS Thermo Fisher Scientific J19943K2 DNAse I Sigma 11284932001 Collagenase D Sigma 11088858001 EDTA (0.5 M), pH 8.0, RNase-free Invitrogen AM9260G Puromycin (hydrochloride) Cayman Chemical 13884 Critical commercial assays MojoSortTM Human CD4 T cell Isolation Kit BioLegend 480010 Human cord blood CD34 isolation kit STEMCELL Technologies 17896 MojoSortTM Human CD8 Nanobeads BioLegend 480108 Human IFN-alpha 2 Quantikine HS ELISA Kit R&D Systems HSIFNA2 Human Total IL-18 DuoSet ELISA R&D Systems DY318-05 P3 Primary Cell 4D-Nucleofector X Kit Lonza V4XP-3032 Quick-RNA Viral Kit Zymo Research R1035 Quick-RNA Microprep Kit Zymo Research R1051 Direct-zol RNA Miniprep Kit Zymo Research R2051 CFSE Cell Division Tracker Kit BioLegend 423801 MitoProbeTM JC-1 Assay Kit Invitrogen M34152 MitoTrackerTM Dyes for Mitochondria Labeling Invitrogen M22426 QIAamp DNA Mini Kit Qiagen 51304 (Continued on next page) Cell Reports Medicine 6, 102311, September 16, 2025 e3 Article ll OPEN ACCESS ..
Reverse Transcription:Article Title: BACH2 promotes seeding and establishment of long-lived HIV-1 reservoir in memory CD4 + T cells.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Zombie Aqua TM Fixable Viability Kit BioLegend 423102 (+)-JQ1 Selleckchem S7110 AZD5153 6-hydroxy-2-naphthoic acid Selleckchem S8344 Ultra-LEAF TM Purified anti-human CD3 Biolegend 300333 Ultra-LEAF TM Purified anti-human CD28 Biolegend 302943 Recombinant Human IL-2 BioLegend 589106 Recombinant Human IL-7 BioLegend 581904 Recombinant Human IL-12(p70) BioLegend 573004 Recombinant Human IL-15 BioLegend 570306 Recombinant Human IL-18 BioLegend 592106 Recombinant Human TNF-α BioLegend 570104 Recombinant Human SCF STEMCELL Technologies 78062 Recombinant Human FLT3 STEMCELL Technologies 78009 Recombinant Human TPO STEMCELL Technologies 78210 Phytohemagglutinin-L (PHA-L) Sigma 11249738001 Enfuvirtide (T-20) MedChemExpress HY-P0052 Raltegravir (RAL) MedChemExpress HY-10353 Recombinant Cas9 protein IDT 1081059 Random Primers Invitrogen 48190–011 ProtoScript II Reverse Transcriptase NEB M0368 RNase Inhibitor, Murine NEB M0314L Oligo(dT)12–18 Primer Invitrogen 18418012 TaqMan TM Fast Advanced Master Mix for qPCR Applied Biosystems 4444556 Lenti-X Concentrator TaKaRa 631232 Ficoll-Paque PLUS GE Healthcare 17144003 Percoll® Sigma GE17-0891-01 HEPARIN Sodium Sagent Pharmaceuticals 25021 Citrate-dextrose solution (ACD) Sigma-Aldrich C3821 RBC Lysis Buffer (10×) Biolegend 420302 Paraformaldehyde Solution, 4% in PBS Thermo Fisher Scientific J19943K2 DNAse I Sigma 11284932001 Collagenase D Sigma 11088858001 EDTA (0.5 M), pH 8.0, RNase-free Invitrogen AM9260G Puromycin (hydrochloride) Cayman Chemical 13884 Critical commercial assays MojoSort TM Human CD4 T cell Isolation Kit BioLegend 480010 Human cord blood CD34 isolation kit STEMCELL Technologies 17896 MojoSort TM Human CD8 Nanobeads BioLegend 480108 Human IFN-alpha 2 Quantikine HS ELISA Kit R&D Systems HSIFNA2 Human Total IL-18 DuoSet ELISA R&D Systems DY318-05 P3 Primary Cell 4D-Nucleofector X Kit Lonza V4XP-3032 Quick-RNA Viral Kit Zymo Research R1035 Quick-RNA Microprep Kit Zymo Research R1051 Direct-zol RNA Miniprep Kit Zymo Research R2051 CFSE Cell Division Tracker Kit BioLegend 423801 MitoProbe TM JC-1 Assay Kit Invitrogen M34152 MitoTracker TM Dyes for Mitochondria Labeling Invitrogen M22426 QIAamp DNA Mini Kit Qiagen 51304 (Continued on next page) Please cite this article in press as: Cell Reports Medicine 6, 102311, September 16, 2025 e3 Article ll OPEN ACCESS ..
Article Title: BACH2 promotes seeding and establishment of long-lived HIV-1 reservoir in memory CD4 + T cells
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Zombie AquaTM Fixable Viability Kit BioLegend 423102 (+)-JQ1 Selleckchem S7110 AZD5153 6-hydroxy-2-naphthoic acid Selleckchem S8344 Ultra-LEAFTM Purified anti-human CD3 Biolegend 300333 Ultra-LEAFTM Purified anti-human CD28 Biolegend 302943 Recombinant Human IL-2 BioLegend 589106 Recombinant Human IL-7 BioLegend 581904 Recombinant Human IL-12(p70) BioLegend 573004 Recombinant Human IL-15 BioLegend 570306 Recombinant Human IL-18 BioLegend 592106 Recombinant Human TNF-α BioLegend 570104 Recombinant Human SCF STEMCELL Technologies 78062 Recombinant Human FLT3 STEMCELL Technologies 78009 Recombinant Human TPO STEMCELL Technologies 78210 Phytohemagglutinin-L (PHA-L) Sigma 11249738001 Enfuvirtide (T-20) MedChemExpress HY-P0052 Raltegravir (RAL) MedChemExpress HY-10353 Recombinant Cas9 protein IDT 1081059 Random Primers Invitrogen 48190–011 ProtoScript II Reverse Transcriptase NEB M0368 RNase Inhibitor, Murine NEB M0314L Oligo(dT)12–18 Primer Invitrogen 18418012 TaqManTM Fast Advanced Master Mix for qPCR Applied Biosystems 4444556 Lenti-X Concentrator TaKaRa 631232 Ficoll-Paque PLUS GE Healthcare 17144003 Percoll® Sigma GE17-0891-01 HEPARIN Sodium Sagent Pharmaceuticals 25021 Citrate-dextrose solution (ACD) Sigma-Aldrich C3821 RBC Lysis Buffer (10×) Biolegend 420302 Paraformaldehyde Solution, 4% in PBS Thermo Fisher Scientific J19943K2 DNAse I Sigma 11284932001 Collagenase D Sigma 11088858001 EDTA (0.5 M), pH 8.0, RNase-free Invitrogen AM9260G Puromycin (hydrochloride) Cayman Chemical 13884 Critical commercial assays MojoSortTM Human CD4 T cell Isolation Kit BioLegend 480010 Human cord blood CD34 isolation kit STEMCELL Technologies 17896 MojoSortTM Human CD8 Nanobeads BioLegend 480108 Human IFN-alpha 2 Quantikine HS ELISA Kit R&D Systems HSIFNA2 Human Total IL-18 DuoSet ELISA R&D Systems DY318-05 P3 Primary Cell 4D-Nucleofector X Kit Lonza V4XP-3032 Quick-RNA Viral Kit Zymo Research R1035 Quick-RNA Microprep Kit Zymo Research R1051 Direct-zol RNA Miniprep Kit Zymo Research R2051 CFSE Cell Division Tracker Kit BioLegend 423801 MitoProbeTM JC-1 Assay Kit Invitrogen M34152 MitoTrackerTM Dyes for Mitochondria Labeling Invitrogen M22426 QIAamp DNA Mini Kit Qiagen 51304 (Continued on next page) Cell Reports Medicine 6, 102311, September 16, 2025 e3 Article ll OPEN ACCESS ..
Real-time Polymerase Chain Reaction:Article Title: BACH2 promotes seeding and establishment of long-lived HIV-1 reservoir in memory CD4 + T cells.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Zombie Aqua TM Fixable Viability Kit BioLegend 423102 (+)-JQ1 Selleckchem S7110 AZD5153 6-hydroxy-2-naphthoic acid Selleckchem S8344 Ultra-LEAF TM Purified anti-human CD3 Biolegend 300333 Ultra-LEAF TM Purified anti-human CD28 Biolegend 302943 Recombinant Human IL-2 BioLegend 589106 Recombinant Human IL-7 BioLegend 581904 Recombinant Human IL-12(p70) BioLegend 573004 Recombinant Human IL-15 BioLegend 570306 Recombinant Human IL-18 BioLegend 592106 Recombinant Human TNF-α BioLegend 570104 Recombinant Human SCF STEMCELL Technologies 78062 Recombinant Human FLT3 STEMCELL Technologies 78009 Recombinant Human TPO STEMCELL Technologies 78210 Phytohemagglutinin-L (PHA-L) Sigma 11249738001 Enfuvirtide (T-20) MedChemExpress HY-P0052 Raltegravir (RAL) MedChemExpress HY-10353 Recombinant Cas9 protein IDT 1081059 Random Primers Invitrogen 48190–011 ProtoScript II Reverse Transcriptase NEB M0368 RNase Inhibitor, Murine NEB M0314L Oligo(dT)12–18 Primer Invitrogen 18418012 TaqMan TM Fast Advanced Master Mix for qPCR Applied Biosystems 4444556 Lenti-X Concentrator TaKaRa 631232 Ficoll-Paque PLUS GE Healthcare 17144003 Percoll® Sigma GE17-0891-01 HEPARIN Sodium Sagent Pharmaceuticals 25021 Citrate-dextrose solution (ACD) Sigma-Aldrich C3821 RBC Lysis Buffer (10×) Biolegend 420302 Paraformaldehyde Solution, 4% in PBS Thermo Fisher Scientific J19943K2 DNAse I Sigma 11284932001 Collagenase D Sigma 11088858001 EDTA (0.5 M), pH 8.0, RNase-free Invitrogen AM9260G Puromycin (hydrochloride) Cayman Chemical 13884 Critical commercial assays MojoSort TM Human CD4 T cell Isolation Kit BioLegend 480010 Human cord blood CD34 isolation kit STEMCELL Technologies 17896 MojoSort TM Human CD8 Nanobeads BioLegend 480108 Human IFN-alpha 2 Quantikine HS ELISA Kit R&D Systems HSIFNA2 Human Total IL-18 DuoSet ELISA R&D Systems DY318-05 P3 Primary Cell 4D-Nucleofector X Kit Lonza V4XP-3032 Quick-RNA Viral Kit Zymo Research R1035 Quick-RNA Microprep Kit Zymo Research R1051 Direct-zol RNA Miniprep Kit Zymo Research R2051 CFSE Cell Division Tracker Kit BioLegend 423801 MitoProbe TM JC-1 Assay Kit Invitrogen M34152 MitoTracker TM Dyes for Mitochondria Labeling Invitrogen M22426 QIAamp DNA Mini Kit Qiagen 51304 (Continued on next page) Please cite this article in press as: Cell Reports Medicine 6, 102311, September 16, 2025 e3 Article ll OPEN ACCESS ..
Article Title: BACH2 promotes seeding and establishment of long-lived HIV-1 reservoir in memory CD4 + T cells
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Zombie AquaTM Fixable Viability Kit BioLegend 423102 (+)-JQ1 Selleckchem S7110 AZD5153 6-hydroxy-2-naphthoic acid Selleckchem S8344 Ultra-LEAFTM Purified anti-human CD3 Biolegend 300333 Ultra-LEAFTM Purified anti-human CD28 Biolegend 302943 Recombinant Human IL-2 BioLegend 589106 Recombinant Human IL-7 BioLegend 581904 Recombinant Human IL-12(p70) BioLegend 573004 Recombinant Human IL-15 BioLegend 570306 Recombinant Human IL-18 BioLegend 592106 Recombinant Human TNF-α BioLegend 570104 Recombinant Human SCF STEMCELL Technologies 78062 Recombinant Human FLT3 STEMCELL Technologies 78009 Recombinant Human TPO STEMCELL Technologies 78210 Phytohemagglutinin-L (PHA-L) Sigma 11249738001 Enfuvirtide (T-20) MedChemExpress HY-P0052 Raltegravir (RAL) MedChemExpress HY-10353 Recombinant Cas9 protein IDT 1081059 Random Primers Invitrogen 48190–011 ProtoScript II Reverse Transcriptase NEB M0368 RNase Inhibitor, Murine NEB M0314L Oligo(dT)12–18 Primer Invitrogen 18418012 TaqManTM Fast Advanced Master Mix for qPCR Applied Biosystems 4444556 Lenti-X Concentrator TaKaRa 631232 Ficoll-Paque PLUS GE Healthcare 17144003 Percoll® Sigma GE17-0891-01 HEPARIN Sodium Sagent Pharmaceuticals 25021 Citrate-dextrose solution (ACD) Sigma-Aldrich C3821 RBC Lysis Buffer (10×) Biolegend 420302 Paraformaldehyde Solution, 4% in PBS Thermo Fisher Scientific J19943K2 DNAse I Sigma 11284932001 Collagenase D Sigma 11088858001 EDTA (0.5 M), pH 8.0, RNase-free Invitrogen AM9260G Puromycin (hydrochloride) Cayman Chemical 13884 Critical commercial assays MojoSortTM Human CD4 T cell Isolation Kit BioLegend 480010 Human cord blood CD34 isolation kit STEMCELL Technologies 17896 MojoSortTM Human CD8 Nanobeads BioLegend 480108 Human IFN-alpha 2 Quantikine HS ELISA Kit R&D Systems HSIFNA2 Human Total IL-18 DuoSet ELISA R&D Systems DY318-05 P3 Primary Cell 4D-Nucleofector X Kit Lonza V4XP-3032 Quick-RNA Viral Kit Zymo Research R1035 Quick-RNA Microprep Kit Zymo Research R1051 Direct-zol RNA Miniprep Kit Zymo Research R2051 CFSE Cell Division Tracker Kit BioLegend 423801 MitoProbeTM JC-1 Assay Kit Invitrogen M34152 MitoTrackerTM Dyes for Mitochondria Labeling Invitrogen M22426 QIAamp DNA Mini Kit Qiagen 51304 (Continued on next page) Cell Reports Medicine 6, 102311, September 16, 2025 e3 Article ll OPEN ACCESS ..
Red Blood Cell Lysis:Article Title: BACH2 promotes seeding and establishment of long-lived HIV-1 reservoir in memory CD4 + T cells.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Zombie Aqua TM Fixable Viability Kit BioLegend 423102 (+)-JQ1 Selleckchem S7110 AZD5153 6-hydroxy-2-naphthoic acid Selleckchem S8344 Ultra-LEAF TM Purified anti-human CD3 Biolegend 300333 Ultra-LEAF TM Purified anti-human CD28 Biolegend 302943 Recombinant Human IL-2 BioLegend 589106 Recombinant Human IL-7 BioLegend 581904 Recombinant Human IL-12(p70) BioLegend 573004 Recombinant Human IL-15 BioLegend 570306 Recombinant Human IL-18 BioLegend 592106 Recombinant Human TNF-α BioLegend 570104 Recombinant Human SCF STEMCELL Technologies 78062 Recombinant Human FLT3 STEMCELL Technologies 78009 Recombinant Human TPO STEMCELL Technologies 78210 Phytohemagglutinin-L (PHA-L) Sigma 11249738001 Enfuvirtide (T-20) MedChemExpress HY-P0052 Raltegravir (RAL) MedChemExpress HY-10353 Recombinant Cas9 protein IDT 1081059 Random Primers Invitrogen 48190–011 ProtoScript II Reverse Transcriptase NEB M0368 RNase Inhibitor, Murine NEB M0314L Oligo(dT)12–18 Primer Invitrogen 18418012 TaqMan TM Fast Advanced Master Mix for qPCR Applied Biosystems 4444556 Lenti-X Concentrator TaKaRa 631232 Ficoll-Paque PLUS GE Healthcare 17144003 Percoll® Sigma GE17-0891-01 HEPARIN Sodium Sagent Pharmaceuticals 25021 Citrate-dextrose solution (ACD) Sigma-Aldrich C3821 RBC Lysis Buffer (10×) Biolegend 420302 Paraformaldehyde Solution, 4% in PBS Thermo Fisher Scientific J19943K2 DNAse I Sigma 11284932001 Collagenase D Sigma 11088858001 EDTA (0.5 M), pH 8.0, RNase-free Invitrogen AM9260G Puromycin (hydrochloride) Cayman Chemical 13884 Critical commercial assays MojoSort TM Human CD4 T cell Isolation Kit BioLegend 480010 Human cord blood CD34 isolation kit STEMCELL Technologies 17896 MojoSort TM Human CD8 Nanobeads BioLegend 480108 Human IFN-alpha 2 Quantikine HS ELISA Kit R&D Systems HSIFNA2 Human Total IL-18 DuoSet ELISA R&D Systems DY318-05 P3 Primary Cell 4D-Nucleofector X Kit Lonza V4XP-3032 Quick-RNA Viral Kit Zymo Research R1035 Quick-RNA Microprep Kit Zymo Research R1051 Direct-zol RNA Miniprep Kit Zymo Research R2051 CFSE Cell Division Tracker Kit BioLegend 423801 MitoProbe TM JC-1 Assay Kit Invitrogen M34152 MitoTracker TM Dyes for Mitochondria Labeling Invitrogen M22426 QIAamp DNA Mini Kit Qiagen 51304 (Continued on next page) Please cite this article in press as: Cell Reports Medicine 6, 102311, September 16, 2025 e3 Article ll OPEN ACCESS ..
Article Title: BACH2 promotes seeding and establishment of long-lived HIV-1 reservoir in memory CD4 + T cells
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Zombie AquaTM Fixable Viability Kit BioLegend 423102 (+)-JQ1 Selleckchem S7110 AZD5153 6-hydroxy-2-naphthoic acid Selleckchem S8344 Ultra-LEAFTM Purified anti-human CD3 Biolegend 300333 Ultra-LEAFTM Purified anti-human CD28 Biolegend 302943 Recombinant Human IL-2 BioLegend 589106 Recombinant Human IL-7 BioLegend 581904 Recombinant Human IL-12(p70) BioLegend 573004 Recombinant Human IL-15 BioLegend 570306 Recombinant Human IL-18 BioLegend 592106 Recombinant Human TNF-α BioLegend 570104 Recombinant Human SCF STEMCELL Technologies 78062 Recombinant Human FLT3 STEMCELL Technologies 78009 Recombinant Human TPO STEMCELL Technologies 78210 Phytohemagglutinin-L (PHA-L) Sigma 11249738001 Enfuvirtide (T-20) MedChemExpress HY-P0052 Raltegravir (RAL) MedChemExpress HY-10353 Recombinant Cas9 protein IDT 1081059 Random Primers Invitrogen 48190–011 ProtoScript II Reverse Transcriptase NEB M0368 RNase Inhibitor, Murine NEB M0314L Oligo(dT)12–18 Primer Invitrogen 18418012 TaqManTM Fast Advanced Master Mix for qPCR Applied Biosystems 4444556 Lenti-X Concentrator TaKaRa 631232 Ficoll-Paque PLUS GE Healthcare 17144003 Percoll® Sigma GE17-0891-01 HEPARIN Sodium Sagent Pharmaceuticals 25021 Citrate-dextrose solution (ACD) Sigma-Aldrich C3821 RBC Lysis Buffer (10×) Biolegend 420302 Paraformaldehyde Solution, 4% in PBS Thermo Fisher Scientific J19943K2 DNAse I Sigma 11284932001 Collagenase D Sigma 11088858001 EDTA (0.5 M), pH 8.0, RNase-free Invitrogen AM9260G Puromycin (hydrochloride) Cayman Chemical 13884 Critical commercial assays MojoSortTM Human CD4 T cell Isolation Kit BioLegend 480010 Human cord blood CD34 isolation kit STEMCELL Technologies 17896 MojoSortTM Human CD8 Nanobeads BioLegend 480108 Human IFN-alpha 2 Quantikine HS ELISA Kit R&D Systems HSIFNA2 Human Total IL-18 DuoSet ELISA R&D Systems DY318-05 P3 Primary Cell 4D-Nucleofector X Kit Lonza V4XP-3032 Quick-RNA Viral Kit Zymo Research R1035 Quick-RNA Microprep Kit Zymo Research R1051 Direct-zol RNA Miniprep Kit Zymo Research R2051 CFSE Cell Division Tracker Kit BioLegend 423801 MitoProbeTM JC-1 Assay Kit Invitrogen M34152 MitoTrackerTM Dyes for Mitochondria Labeling Invitrogen M22426 QIAamp DNA Mini Kit Qiagen 51304 (Continued on next page) Cell Reports Medicine 6, 102311, September 16, 2025 e3 Article ll OPEN ACCESS ..
Cell Isolation:Article Title: BACH2 promotes seeding and establishment of long-lived HIV-1 reservoir in memory CD4 + T cells.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Zombie Aqua TM Fixable Viability Kit BioLegend 423102 (+)-JQ1 Selleckchem S7110 AZD5153 6-hydroxy-2-naphthoic acid Selleckchem S8344 Ultra-LEAF TM Purified anti-human CD3 Biolegend 300333 Ultra-LEAF TM Purified anti-human CD28 Biolegend 302943 Recombinant Human IL-2 BioLegend 589106 Recombinant Human IL-7 BioLegend 581904 Recombinant Human IL-12(p70) BioLegend 573004 Recombinant Human IL-15 BioLegend 570306 Recombinant Human IL-18 BioLegend 592106 Recombinant Human TNF-α BioLegend 570104 Recombinant Human SCF STEMCELL Technologies 78062 Recombinant Human FLT3 STEMCELL Technologies 78009 Recombinant Human TPO STEMCELL Technologies 78210 Phytohemagglutinin-L (PHA-L) Sigma 11249738001 Enfuvirtide (T-20) MedChemExpress HY-P0052 Raltegravir (RAL) MedChemExpress HY-10353 Recombinant Cas9 protein IDT 1081059 Random Primers Invitrogen 48190–011 ProtoScript II Reverse Transcriptase NEB M0368 RNase Inhibitor, Murine NEB M0314L Oligo(dT)12–18 Primer Invitrogen 18418012 TaqMan TM Fast Advanced Master Mix for qPCR Applied Biosystems 4444556 Lenti-X Concentrator TaKaRa 631232 Ficoll-Paque PLUS GE Healthcare 17144003 Percoll® Sigma GE17-0891-01 HEPARIN Sodium Sagent Pharmaceuticals 25021 Citrate-dextrose solution (ACD) Sigma-Aldrich C3821 RBC Lysis Buffer (10×) Biolegend 420302 Paraformaldehyde Solution, 4% in PBS Thermo Fisher Scientific J19943K2 DNAse I Sigma 11284932001 Collagenase D Sigma 11088858001 EDTA (0.5 M), pH 8.0, RNase-free Invitrogen AM9260G Puromycin (hydrochloride) Cayman Chemical 13884 Critical commercial assays MojoSort TM Human CD4 T cell Isolation Kit BioLegend 480010 Human cord blood CD34 isolation kit STEMCELL Technologies 17896 MojoSort TM Human CD8 Nanobeads BioLegend 480108 Human IFN-alpha 2 Quantikine HS ELISA Kit R&D Systems HSIFNA2 Human Total IL-18 DuoSet ELISA R&D Systems DY318-05 P3 Primary Cell 4D-Nucleofector X Kit Lonza V4XP-3032 Quick-RNA Viral Kit Zymo Research R1035 Quick-RNA Microprep Kit Zymo Research R1051 Direct-zol RNA Miniprep Kit Zymo Research R2051 CFSE Cell Division Tracker Kit BioLegend 423801 MitoProbe TM JC-1 Assay Kit Invitrogen M34152 MitoTracker TM Dyes for Mitochondria Labeling Invitrogen M22426 QIAamp DNA Mini Kit Qiagen 51304 (Continued on next page) Please cite this article in press as: Cell Reports Medicine 6, 102311, September 16, 2025 e3 Article ll OPEN ACCESS ..
Article Title: BACH2 promotes seeding and establishment of long-lived HIV-1 reservoir in memory CD4 + T cells
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Zombie AquaTM Fixable Viability Kit BioLegend 423102 (+)-JQ1 Selleckchem S7110 AZD5153 6-hydroxy-2-naphthoic acid Selleckchem S8344 Ultra-LEAFTM Purified anti-human CD3 Biolegend 300333 Ultra-LEAFTM Purified anti-human CD28 Biolegend 302943 Recombinant Human IL-2 BioLegend 589106 Recombinant Human IL-7 BioLegend 581904 Recombinant Human IL-12(p70) BioLegend 573004 Recombinant Human IL-15 BioLegend 570306 Recombinant Human IL-18 BioLegend 592106 Recombinant Human TNF-α BioLegend 570104 Recombinant Human SCF STEMCELL Technologies 78062 Recombinant Human FLT3 STEMCELL Technologies 78009 Recombinant Human TPO STEMCELL Technologies 78210 Phytohemagglutinin-L (PHA-L) Sigma 11249738001 Enfuvirtide (T-20) MedChemExpress HY-P0052 Raltegravir (RAL) MedChemExpress HY-10353 Recombinant Cas9 protein IDT 1081059 Random Primers Invitrogen 48190–011 ProtoScript II Reverse Transcriptase NEB M0368 RNase Inhibitor, Murine NEB M0314L Oligo(dT)12–18 Primer Invitrogen 18418012 TaqManTM Fast Advanced Master Mix for qPCR Applied Biosystems 4444556 Lenti-X Concentrator TaKaRa 631232 Ficoll-Paque PLUS GE Healthcare 17144003 Percoll® Sigma GE17-0891-01 HEPARIN Sodium Sagent Pharmaceuticals 25021 Citrate-dextrose solution (ACD) Sigma-Aldrich C3821 RBC Lysis Buffer (10×) Biolegend 420302 Paraformaldehyde Solution, 4% in PBS Thermo Fisher Scientific J19943K2 DNAse I Sigma 11284932001 Collagenase D Sigma 11088858001 EDTA (0.5 M), pH 8.0, RNase-free Invitrogen AM9260G Puromycin (hydrochloride) Cayman Chemical 13884 Critical commercial assays MojoSortTM Human CD4 T cell Isolation Kit BioLegend 480010 Human cord blood CD34 isolation kit STEMCELL Technologies 17896 MojoSortTM Human CD8 Nanobeads BioLegend 480108 Human IFN-alpha 2 Quantikine HS ELISA Kit R&D Systems HSIFNA2 Human Total IL-18 DuoSet ELISA R&D Systems DY318-05 P3 Primary Cell 4D-Nucleofector X Kit Lonza V4XP-3032 Quick-RNA Viral Kit Zymo Research R1035 Quick-RNA Microprep Kit Zymo Research R1051 Direct-zol RNA Miniprep Kit Zymo Research R2051 CFSE Cell Division Tracker Kit BioLegend 423801 MitoProbeTM JC-1 Assay Kit Invitrogen M34152 MitoTrackerTM Dyes for Mitochondria Labeling Invitrogen M22426 QIAamp DNA Mini Kit Qiagen 51304 (Continued on next page) Cell Reports Medicine 6, 102311, September 16, 2025 e3 Article ll OPEN ACCESS ..
Isolation:Article Title: BACH2 promotes seeding and establishment of long-lived HIV-1 reservoir in memory CD4 + T cells.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Zombie Aqua TM Fixable Viability Kit BioLegend 423102 (+)-JQ1 Selleckchem S7110 AZD5153 6-hydroxy-2-naphthoic acid Selleckchem S8344 Ultra-LEAF TM Purified anti-human CD3 Biolegend 300333 Ultra-LEAF TM Purified anti-human CD28 Biolegend 302943 Recombinant Human IL-2 BioLegend 589106 Recombinant Human IL-7 BioLegend 581904 Recombinant Human IL-12(p70) BioLegend 573004 Recombinant Human IL-15 BioLegend 570306 Recombinant Human IL-18 BioLegend 592106 Recombinant Human TNF-α BioLegend 570104 Recombinant Human SCF STEMCELL Technologies 78062 Recombinant Human FLT3 STEMCELL Technologies 78009 Recombinant Human TPO STEMCELL Technologies 78210 Phytohemagglutinin-L (PHA-L) Sigma 11249738001 Enfuvirtide (T-20) MedChemExpress HY-P0052 Raltegravir (RAL) MedChemExpress HY-10353 Recombinant Cas9 protein IDT 1081059 Random Primers Invitrogen 48190–011 ProtoScript II Reverse Transcriptase NEB M0368 RNase Inhibitor, Murine NEB M0314L Oligo(dT)12–18 Primer Invitrogen 18418012 TaqMan TM Fast Advanced Master Mix for qPCR Applied Biosystems 4444556 Lenti-X Concentrator TaKaRa 631232 Ficoll-Paque PLUS GE Healthcare 17144003 Percoll® Sigma GE17-0891-01 HEPARIN Sodium Sagent Pharmaceuticals 25021 Citrate-dextrose solution (ACD) Sigma-Aldrich C3821 RBC Lysis Buffer (10×) Biolegend 420302 Paraformaldehyde Solution, 4% in PBS Thermo Fisher Scientific J19943K2 DNAse I Sigma 11284932001 Collagenase D Sigma 11088858001 EDTA (0.5 M), pH 8.0, RNase-free Invitrogen AM9260G Puromycin (hydrochloride) Cayman Chemical 13884 Critical commercial assays MojoSort TM Human CD4 T cell Isolation Kit BioLegend 480010 Human cord blood CD34 isolation kit STEMCELL Technologies 17896 MojoSort TM Human CD8 Nanobeads BioLegend 480108 Human IFN-alpha 2 Quantikine HS ELISA Kit R&D Systems HSIFNA2 Human Total IL-18 DuoSet ELISA R&D Systems DY318-05 P3 Primary Cell 4D-Nucleofector X Kit Lonza V4XP-3032 Quick-RNA Viral Kit Zymo Research R1035 Quick-RNA Microprep Kit Zymo Research R1051 Direct-zol RNA Miniprep Kit Zymo Research R2051 CFSE Cell Division Tracker Kit BioLegend 423801 MitoProbe TM JC-1 Assay Kit Invitrogen M34152 MitoTracker TM Dyes for Mitochondria Labeling Invitrogen M22426 QIAamp DNA Mini Kit Qiagen 51304 (Continued on next page) Please cite this article in press as: Cell Reports Medicine 6, 102311, September 16, 2025 e3 Article ll OPEN ACCESS ..
Article Title: BACH2 promotes seeding and establishment of long-lived HIV-1 reservoir in memory CD4 + T cells
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Zombie AquaTM Fixable Viability Kit BioLegend 423102 (+)-JQ1 Selleckchem S7110 AZD5153 6-hydroxy-2-naphthoic acid Selleckchem S8344 Ultra-LEAFTM Purified anti-human CD3 Biolegend 300333 Ultra-LEAFTM Purified anti-human CD28 Biolegend 302943 Recombinant Human IL-2 BioLegend 589106 Recombinant Human IL-7 BioLegend 581904 Recombinant Human IL-12(p70) BioLegend 573004 Recombinant Human IL-15 BioLegend 570306 Recombinant Human IL-18 BioLegend 592106 Recombinant Human TNF-α BioLegend 570104 Recombinant Human SCF STEMCELL Technologies 78062 Recombinant Human FLT3 STEMCELL Technologies 78009 Recombinant Human TPO STEMCELL Technologies 78210 Phytohemagglutinin-L (PHA-L) Sigma 11249738001 Enfuvirtide (T-20) MedChemExpress HY-P0052 Raltegravir (RAL) MedChemExpress HY-10353 Recombinant Cas9 protein IDT 1081059 Random Primers Invitrogen 48190–011 ProtoScript II Reverse Transcriptase NEB M0368 RNase Inhibitor, Murine NEB M0314L Oligo(dT)12–18 Primer Invitrogen 18418012 TaqManTM Fast Advanced Master Mix for qPCR Applied Biosystems 4444556 Lenti-X Concentrator TaKaRa 631232 Ficoll-Paque PLUS GE Healthcare 17144003 Percoll® Sigma GE17-0891-01 HEPARIN Sodium Sagent Pharmaceuticals 25021 Citrate-dextrose solution (ACD) Sigma-Aldrich C3821 RBC Lysis Buffer (10×) Biolegend 420302 Paraformaldehyde Solution, 4% in PBS Thermo Fisher Scientific J19943K2 DNAse I Sigma 11284932001 Collagenase D Sigma 11088858001 EDTA (0.5 M), pH 8.0, RNase-free Invitrogen AM9260G Puromycin (hydrochloride) Cayman Chemical 13884 Critical commercial assays MojoSortTM Human CD4 T cell Isolation Kit BioLegend 480010 Human cord blood CD34 isolation kit STEMCELL Technologies 17896 MojoSortTM Human CD8 Nanobeads BioLegend 480108 Human IFN-alpha 2 Quantikine HS ELISA Kit R&D Systems HSIFNA2 Human Total IL-18 DuoSet ELISA R&D Systems DY318-05 P3 Primary Cell 4D-Nucleofector X Kit Lonza V4XP-3032 Quick-RNA Viral Kit Zymo Research R1035 Quick-RNA Microprep Kit Zymo Research R1051 Direct-zol RNA Miniprep Kit Zymo Research R2051 CFSE Cell Division Tracker Kit BioLegend 423801 MitoProbeTM JC-1 Assay Kit Invitrogen M34152 MitoTrackerTM Dyes for Mitochondria Labeling Invitrogen M22426 QIAamp DNA Mini Kit Qiagen 51304 (Continued on next page) Cell Reports Medicine 6, 102311, September 16, 2025 e3 Article ll OPEN ACCESS ..
Enzyme-linked Immunosorbent Assay:Article Title: BACH2 promotes seeding and establishment of long-lived HIV-1 reservoir in memory CD4 + T cells.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Zombie Aqua TM Fixable Viability Kit BioLegend 423102 (+)-JQ1 Selleckchem S7110 AZD5153 6-hydroxy-2-naphthoic acid Selleckchem S8344 Ultra-LEAF TM Purified anti-human CD3 Biolegend 300333 Ultra-LEAF TM Purified anti-human CD28 Biolegend 302943 Recombinant Human IL-2 BioLegend 589106 Recombinant Human IL-7 BioLegend 581904 Recombinant Human IL-12(p70) BioLegend 573004 Recombinant Human IL-15 BioLegend 570306 Recombinant Human IL-18 BioLegend 592106 Recombinant Human TNF-α BioLegend 570104 Recombinant Human SCF STEMCELL Technologies 78062 Recombinant Human FLT3 STEMCELL Technologies 78009 Recombinant Human TPO STEMCELL Technologies 78210 Phytohemagglutinin-L (PHA-L) Sigma 11249738001 Enfuvirtide (T-20) MedChemExpress HY-P0052 Raltegravir (RAL) MedChemExpress HY-10353 Recombinant Cas9 protein IDT 1081059 Random Primers Invitrogen 48190–011 ProtoScript II Reverse Transcriptase NEB M0368 RNase Inhibitor, Murine NEB M0314L Oligo(dT)12–18 Primer Invitrogen 18418012 TaqMan TM Fast Advanced Master Mix for qPCR Applied Biosystems 4444556 Lenti-X Concentrator TaKaRa 631232 Ficoll-Paque PLUS GE Healthcare 17144003 Percoll® Sigma GE17-0891-01 HEPARIN Sodium Sagent Pharmaceuticals 25021 Citrate-dextrose solution (ACD) Sigma-Aldrich C3821 RBC Lysis Buffer (10×) Biolegend 420302 Paraformaldehyde Solution, 4% in PBS Thermo Fisher Scientific J19943K2 DNAse I Sigma 11284932001 Collagenase D Sigma 11088858001 EDTA (0.5 M), pH 8.0, RNase-free Invitrogen AM9260G Puromycin (hydrochloride) Cayman Chemical 13884 Critical commercial assays MojoSort TM Human CD4 T cell Isolation Kit BioLegend 480010 Human cord blood CD34 isolation kit STEMCELL Technologies 17896 MojoSort TM Human CD8 Nanobeads BioLegend 480108 Human IFN-alpha 2 Quantikine HS ELISA Kit R&D Systems HSIFNA2 Human Total IL-18 DuoSet ELISA R&D Systems DY318-05 P3 Primary Cell 4D-Nucleofector X Kit Lonza V4XP-3032 Quick-RNA Viral Kit Zymo Research R1035 Quick-RNA Microprep Kit Zymo Research R1051 Direct-zol RNA Miniprep Kit Zymo Research R2051 CFSE Cell Division Tracker Kit BioLegend 423801 MitoProbe TM JC-1 Assay Kit Invitrogen M34152 MitoTracker TM Dyes for Mitochondria Labeling Invitrogen M22426 QIAamp DNA Mini Kit Qiagen 51304 (Continued on next page) Please cite this article in press as: Cell Reports Medicine 6, 102311, September 16, 2025 e3 Article ll OPEN ACCESS ..
Article Title: BACH2 promotes seeding and establishment of long-lived HIV-1 reservoir in memory CD4 + T cells
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Zombie AquaTM Fixable Viability Kit BioLegend 423102 (+)-JQ1 Selleckchem S7110 AZD5153 6-hydroxy-2-naphthoic acid Selleckchem S8344 Ultra-LEAFTM Purified anti-human CD3 Biolegend 300333 Ultra-LEAFTM Purified anti-human CD28 Biolegend 302943 Recombinant Human IL-2 BioLegend 589106 Recombinant Human IL-7 BioLegend 581904 Recombinant Human IL-12(p70) BioLegend 573004 Recombinant Human IL-15 BioLegend 570306 Recombinant Human IL-18 BioLegend 592106 Recombinant Human TNF-α BioLegend 570104 Recombinant Human SCF STEMCELL Technologies 78062 Recombinant Human FLT3 STEMCELL Technologies 78009 Recombinant Human TPO STEMCELL Technologies 78210 Phytohemagglutinin-L (PHA-L) Sigma 11249738001 Enfuvirtide (T-20) MedChemExpress HY-P0052 Raltegravir (RAL) MedChemExpress HY-10353 Recombinant Cas9 protein IDT 1081059 Random Primers Invitrogen 48190–011 ProtoScript II Reverse Transcriptase NEB M0368 RNase Inhibitor, Murine NEB M0314L Oligo(dT)12–18 Primer Invitrogen 18418012 TaqManTM Fast Advanced Master Mix for qPCR Applied Biosystems 4444556 Lenti-X Concentrator TaKaRa 631232 Ficoll-Paque PLUS GE Healthcare 17144003 Percoll® Sigma GE17-0891-01 HEPARIN Sodium Sagent Pharmaceuticals 25021 Citrate-dextrose solution (ACD) Sigma-Aldrich C3821 RBC Lysis Buffer (10×) Biolegend 420302 Paraformaldehyde Solution, 4% in PBS Thermo Fisher Scientific J19943K2 DNAse I Sigma 11284932001 Collagenase D Sigma 11088858001 EDTA (0.5 M), pH 8.0, RNase-free Invitrogen AM9260G Puromycin (hydrochloride) Cayman Chemical 13884 Critical commercial assays MojoSortTM Human CD4 T cell Isolation Kit BioLegend 480010 Human cord blood CD34 isolation kit STEMCELL Technologies 17896 MojoSortTM Human CD8 Nanobeads BioLegend 480108 Human IFN-alpha 2 Quantikine HS ELISA Kit R&D Systems HSIFNA2 Human Total IL-18 DuoSet ELISA R&D Systems DY318-05 P3 Primary Cell 4D-Nucleofector X Kit Lonza V4XP-3032 Quick-RNA Viral Kit Zymo Research R1035 Quick-RNA Microprep Kit Zymo Research R1051 Direct-zol RNA Miniprep Kit Zymo Research R2051 CFSE Cell Division Tracker Kit BioLegend 423801 MitoProbeTM JC-1 Assay Kit Invitrogen M34152 MitoTrackerTM Dyes for Mitochondria Labeling Invitrogen M22426 QIAamp DNA Mini Kit Qiagen 51304 (Continued on next page) Cell Reports Medicine 6, 102311, September 16, 2025 e3 Article ll OPEN ACCESS ..
Labeling:Article Title: BACH2 promotes seeding and establishment of long-lived HIV-1 reservoir in memory CD4 + T cells.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Zombie Aqua TM Fixable Viability Kit BioLegend 423102 (+)-JQ1 Selleckchem S7110 AZD5153 6-hydroxy-2-naphthoic acid Selleckchem S8344 Ultra-LEAF TM Purified anti-human CD3 Biolegend 300333 Ultra-LEAF TM Purified anti-human CD28 Biolegend 302943 Recombinant Human IL-2 BioLegend 589106 Recombinant Human IL-7 BioLegend 581904 Recombinant Human IL-12(p70) BioLegend 573004 Recombinant Human IL-15 BioLegend 570306 Recombinant Human IL-18 BioLegend 592106 Recombinant Human TNF-α BioLegend 570104 Recombinant Human SCF STEMCELL Technologies 78062 Recombinant Human FLT3 STEMCELL Technologies 78009 Recombinant Human TPO STEMCELL Technologies 78210 Phytohemagglutinin-L (PHA-L) Sigma 11249738001 Enfuvirtide (T-20) MedChemExpress HY-P0052 Raltegravir (RAL) MedChemExpress HY-10353 Recombinant Cas9 protein IDT 1081059 Random Primers Invitrogen 48190–011 ProtoScript II Reverse Transcriptase NEB M0368 RNase Inhibitor, Murine NEB M0314L Oligo(dT)12–18 Primer Invitrogen 18418012 TaqMan TM Fast Advanced Master Mix for qPCR Applied Biosystems 4444556 Lenti-X Concentrator TaKaRa 631232 Ficoll-Paque PLUS GE Healthcare 17144003 Percoll® Sigma GE17-0891-01 HEPARIN Sodium Sagent Pharmaceuticals 25021 Citrate-dextrose solution (ACD) Sigma-Aldrich C3821 RBC Lysis Buffer (10×) Biolegend 420302 Paraformaldehyde Solution, 4% in PBS Thermo Fisher Scientific J19943K2 DNAse I Sigma 11284932001 Collagenase D Sigma 11088858001 EDTA (0.5 M), pH 8.0, RNase-free Invitrogen AM9260G Puromycin (hydrochloride) Cayman Chemical 13884 Critical commercial assays MojoSort TM Human CD4 T cell Isolation Kit BioLegend 480010 Human cord blood CD34 isolation kit STEMCELL Technologies 17896 MojoSort TM Human CD8 Nanobeads BioLegend 480108 Human IFN-alpha 2 Quantikine HS ELISA Kit R&D Systems HSIFNA2 Human Total IL-18 DuoSet ELISA R&D Systems DY318-05 P3 Primary Cell 4D-Nucleofector X Kit Lonza V4XP-3032 Quick-RNA Viral Kit Zymo Research R1035 Quick-RNA Microprep Kit Zymo Research R1051 Direct-zol RNA Miniprep Kit Zymo Research R2051 CFSE Cell Division Tracker Kit BioLegend 423801 MitoProbe TM JC-1 Assay Kit Invitrogen M34152 MitoTracker TM Dyes for Mitochondria Labeling Invitrogen M22426 QIAamp DNA Mini Kit Qiagen 51304 (Continued on next page) Please cite this article in press as: Cell Reports Medicine 6, 102311, September 16, 2025 e3 Article ll OPEN ACCESS ..
Article Title: BACH2 promotes seeding and establishment of long-lived HIV-1 reservoir in memory CD4 + T cells
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Zombie AquaTM Fixable Viability Kit BioLegend 423102 (+)-JQ1 Selleckchem S7110 AZD5153 6-hydroxy-2-naphthoic acid Selleckchem S8344 Ultra-LEAFTM Purified anti-human CD3 Biolegend 300333 Ultra-LEAFTM Purified anti-human CD28 Biolegend 302943 Recombinant Human IL-2 BioLegend 589106 Recombinant Human IL-7 BioLegend 581904 Recombinant Human IL-12(p70) BioLegend 573004 Recombinant Human IL-15 BioLegend 570306 Recombinant Human IL-18 BioLegend 592106 Recombinant Human TNF-α BioLegend 570104 Recombinant Human SCF STEMCELL Technologies 78062 Recombinant Human FLT3 STEMCELL Technologies 78009 Recombinant Human TPO STEMCELL Technologies 78210 Phytohemagglutinin-L (PHA-L) Sigma 11249738001 Enfuvirtide (T-20) MedChemExpress HY-P0052 Raltegravir (RAL) MedChemExpress HY-10353 Recombinant Cas9 protein IDT 1081059 Random Primers Invitrogen 48190–011 ProtoScript II Reverse Transcriptase NEB M0368 RNase Inhibitor, Murine NEB M0314L Oligo(dT)12–18 Primer Invitrogen 18418012 TaqManTM Fast Advanced Master Mix for qPCR Applied Biosystems 4444556 Lenti-X Concentrator TaKaRa 631232 Ficoll-Paque PLUS GE Healthcare 17144003 Percoll® Sigma GE17-0891-01 HEPARIN Sodium Sagent Pharmaceuticals 25021 Citrate-dextrose solution (ACD) Sigma-Aldrich C3821 RBC Lysis Buffer (10×) Biolegend 420302 Paraformaldehyde Solution, 4% in PBS Thermo Fisher Scientific J19943K2 DNAse I Sigma 11284932001 Collagenase D Sigma 11088858001 EDTA (0.5 M), pH 8.0, RNase-free Invitrogen AM9260G Puromycin (hydrochloride) Cayman Chemical 13884 Critical commercial assays MojoSortTM Human CD4 T cell Isolation Kit BioLegend 480010 Human cord blood CD34 isolation kit STEMCELL Technologies 17896 MojoSortTM Human CD8 Nanobeads BioLegend 480108 Human IFN-alpha 2 Quantikine HS ELISA Kit R&D Systems HSIFNA2 Human Total IL-18 DuoSet ELISA R&D Systems DY318-05 P3 Primary Cell 4D-Nucleofector X Kit Lonza V4XP-3032 Quick-RNA Viral Kit Zymo Research R1035 Quick-RNA Microprep Kit Zymo Research R1051 Direct-zol RNA Miniprep Kit Zymo Research R2051 CFSE Cell Division Tracker Kit BioLegend 423801 MitoProbeTM JC-1 Assay Kit Invitrogen M34152 MitoTrackerTM Dyes for Mitochondria Labeling Invitrogen M22426 QIAamp DNA Mini Kit Qiagen 51304 (Continued on next page) Cell Reports Medicine 6, 102311, September 16, 2025 e3 Article ll OPEN ACCESS ..
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