Virus:Article Title: The BZR1-interacting WD repeat protein participates in brassinosteroid-dependent growth and stress response in tomato.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-Actin ImmunoWay Cat. #YM3034 anti-Flag Invitrogen Cat. # FG4R; RRID: AB_1957945 anti-GFP Invitrogen Cat. # MA5-15256; RRID: AB_10979281 anti-GST ABclonal Cat. # AE001; RRID: AB_2770403 anti-HA Invitrogen Cat. # 26183; RRID: AB_10978021 anti-His ABclonal Cat. # AE003; RRID: AB_2728734 anti-mouse ABclonal Cat. # AS003; RRID: AB_2769851 anti-Myc ABclonal Cat. # AE010; RRID: AB_2770408 anti-rabbit ABclonal Cat. # AS014; RRID: AB_2769854 anti-Ser/Thr ECM Cat. # PM3801; RRID: AB_1944434 Bacterial and virus strains Agrobacterium tumefaciens strain GV3101 Weidi-Bio Cat. # AE1003 Escherichia coli BL21 (DE3) TransGen Biotech Cat. # CD801-02 A. Rhizobiaceae strain C58C1 Huayueyang Cat. # hyyd-10*100μl Escherichia coli Trans 5α TransGen Biotech Cat. # CD201-01 Biological samples Solanum lycopersicum: Leaf, root, stem, apical bud, lateral bud, fruit This study N/A Nicotiana benthamiana: Leaf This study N/A Chemicals, peptides, and recombinant proteins GFP-Trap magnetic beads Chromotek Cat. # AB_2631358 HA magnetic beads MCE Cat. # HY-K0201 Flag magnetic beads Merck Cat. #M8823-1ML Ni-NTA agarose Qiagen Cat. # 30210 Glutathione agarose Thermo Fisher Cat. # 16100 HBR MCE Cat. # HY-N9435 BRZ Sigma Cat. # SML1406 Bikinin MCE Cat. # HY12524 SD-Trp-Leu medium Coolaber Cat. # PM2222 SD-Ade-His-Leu-Trp medium Coolaber Cat. # PM2112 MG132 Abcam Cat. # ab141003 CHX MCE Cat. # HY-12320 BSA FD-Bio Cat. # FD0030 Critical commercial assays RNA prep pure Plant Kit Tiangen Cat. # DP419 ChamQ Universal SYBR qPCR Master Mix vazyme Cat. #Q711-02/03 HiScript II QRT SuperMix for qPCR vazyme Cat. #R223-01 ClonExpress® II One Step Cloning Kit vazyme Cat. #C112-01 FDbio-Femto Ecl Kit FD-Bio Cat. # FD8030 YeastmakerTM Yeast Transformation System 2 Clontech Cat. # 630439 Deposited data RNA-seq data This paper GSA accession: CRA033954 Experimental models: Organisms/strains S. lycopersicum: biw This paper N/A (Continued on next page) Cell Reports 45, 116876, February 24, 2026 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER S. lycopersicum: OE-BIW-HA This paper N/A S. lycopersicum: OE-BIWA-HA This paper N/A S. lycopersicum: biw/bzr1 This paper N/A S. lycopersicum: bzr1 Yin et al.77 N/A S. lycopersicum: OE-BZR1-HA Yin et al.77 N/A S. lycopersicum: OE-BIN2-HA An et al.28 N/A S. lycopersicum: dwf Li et al.53 N/A Oligonucleotides Primers used in this study This paper N/A Recombinant DNA AD-BIW This paper N/A BD-BIW This paper N/A BIW-HA This paper N/A BIWA-HA This paper N/A BIW-GFP This paper N/A BIWA-GFP This paper N/A BIWD-GFP This paper N/A BIW-Myc This paper N/A BIW-nYFP This paper N/A BIW-cYFP This paper N/A His-BIW This paper N/A AD-BZR1 This paper N/A AD-BZR1-N This paper N/A AD-BZR1-C This paper N/A BZR1-nYFP This paper N/A BZR1-cYFP This paper N/A BZR1-HA This paper N/A GST-BZR1-C This paper N/A BD-BIN2 This paper N/A BIN2-nYFP This paper N/A BIN2-cYFP This paper N/A GST-BIN2 This paper N/A BIN2-GFP This paper N/A UBQ-Flag This paper N/A DDB1-HA This paper N/A DDB1-GFP This paper N/A DDB1-nYFP This paper N/A CUL4-HA This paper N/A CUL4-GFP This paper N/A CUL4-nYFP This paper N/A BES1-nYFP This paper N/A BES1-cYFP This paper N/A D14-nYFP This paper N/A D14-cYFP This paper N/A Software and algorithms GraphPad 9.0 GraphPad Software https://www.graphpad.com ImageJ NIH https://imagej.net/ij/ Virus-induced gene silencing (VIGS) tool VIGS https://vigs.solgenomics.net/ 18 Cell Reports 45, 116876, February 24, 2026
Recombinant:Article Title: The BZR1-interacting WD repeat protein participates in brassinosteroid-dependent growth and stress response in tomato.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-Actin ImmunoWay Cat. #YM3034 anti-Flag Invitrogen Cat. # FG4R; RRID: AB_1957945 anti-GFP Invitrogen Cat. # MA5-15256; RRID: AB_10979281 anti-GST ABclonal Cat. # AE001; RRID: AB_2770403 anti-HA Invitrogen Cat. # 26183; RRID: AB_10978021 anti-His ABclonal Cat. # AE003; RRID: AB_2728734 anti-mouse ABclonal Cat. # AS003; RRID: AB_2769851 anti-Myc ABclonal Cat. # AE010; RRID: AB_2770408 anti-rabbit ABclonal Cat. # AS014; RRID: AB_2769854 anti-Ser/Thr ECM Cat. # PM3801; RRID: AB_1944434 Bacterial and virus strains Agrobacterium tumefaciens strain GV3101 Weidi-Bio Cat. # AE1003 Escherichia coli BL21 (DE3) TransGen Biotech Cat. # CD801-02 A. Rhizobiaceae strain C58C1 Huayueyang Cat. # hyyd-10*100μl Escherichia coli Trans 5α TransGen Biotech Cat. # CD201-01 Biological samples Solanum lycopersicum: Leaf, root, stem, apical bud, lateral bud, fruit This study N/A Nicotiana benthamiana: Leaf This study N/A Chemicals, peptides, and recombinant proteins GFP-Trap magnetic beads Chromotek Cat. # AB_2631358 HA magnetic beads MCE Cat. # HY-K0201 Flag magnetic beads Merck Cat. #M8823-1ML Ni-NTA agarose Qiagen Cat. # 30210 Glutathione agarose Thermo Fisher Cat. # 16100 HBR MCE Cat. # HY-N9435 BRZ Sigma Cat. # SML1406 Bikinin MCE Cat. # HY12524 SD-Trp-Leu medium Coolaber Cat. # PM2222 SD-Ade-His-Leu-Trp medium Coolaber Cat. # PM2112 MG132 Abcam Cat. # ab141003 CHX MCE Cat. # HY-12320 BSA FD-Bio Cat. # FD0030 Critical commercial assays RNA prep pure Plant Kit Tiangen Cat. # DP419 ChamQ Universal SYBR qPCR Master Mix vazyme Cat. #Q711-02/03 HiScript II QRT SuperMix for qPCR vazyme Cat. #R223-01 ClonExpress® II One Step Cloning Kit vazyme Cat. #C112-01 FDbio-Femto Ecl Kit FD-Bio Cat. # FD8030 YeastmakerTM Yeast Transformation System 2 Clontech Cat. # 630439 Deposited data RNA-seq data This paper GSA accession: CRA033954 Experimental models: Organisms/strains S. lycopersicum: biw This paper N/A (Continued on next page) Cell Reports 45, 116876, February 24, 2026 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER S. lycopersicum: OE-BIW-HA This paper N/A S. lycopersicum: OE-BIWA-HA This paper N/A S. lycopersicum: biw/bzr1 This paper N/A S. lycopersicum: bzr1 Yin et al.77 N/A S. lycopersicum: OE-BZR1-HA Yin et al.77 N/A S. lycopersicum: OE-BIN2-HA An et al.28 N/A S. lycopersicum: dwf Li et al.53 N/A Oligonucleotides Primers used in this study This paper N/A Recombinant DNA AD-BIW This paper N/A BD-BIW This paper N/A BIW-HA This paper N/A BIWA-HA This paper N/A BIW-GFP This paper N/A BIWA-GFP This paper N/A BIWD-GFP This paper N/A BIW-Myc This paper N/A BIW-nYFP This paper N/A BIW-cYFP This paper N/A His-BIW This paper N/A AD-BZR1 This paper N/A AD-BZR1-N This paper N/A AD-BZR1-C This paper N/A BZR1-nYFP This paper N/A BZR1-cYFP This paper N/A BZR1-HA This paper N/A GST-BZR1-C This paper N/A BD-BIN2 This paper N/A BIN2-nYFP This paper N/A BIN2-cYFP This paper N/A GST-BIN2 This paper N/A BIN2-GFP This paper N/A UBQ-Flag This paper N/A DDB1-HA This paper N/A DDB1-GFP This paper N/A DDB1-nYFP This paper N/A CUL4-HA This paper N/A CUL4-GFP This paper N/A CUL4-nYFP This paper N/A BES1-nYFP This paper N/A BES1-cYFP This paper N/A D14-nYFP This paper N/A D14-cYFP This paper N/A Software and algorithms GraphPad 9.0 GraphPad Software https://www.graphpad.com ImageJ NIH https://imagej.net/ij/ Virus-induced gene silencing (VIGS) tool VIGS https://vigs.solgenomics.net/ 18 Cell Reports 45, 116876, February 24, 2026
Magnetic Beads:Article Title: The BZR1-interacting WD repeat protein participates in brassinosteroid-dependent growth and stress response in tomato.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-Actin ImmunoWay Cat. #YM3034 anti-Flag Invitrogen Cat. # FG4R; RRID: AB_1957945 anti-GFP Invitrogen Cat. # MA5-15256; RRID: AB_10979281 anti-GST ABclonal Cat. # AE001; RRID: AB_2770403 anti-HA Invitrogen Cat. # 26183; RRID: AB_10978021 anti-His ABclonal Cat. # AE003; RRID: AB_2728734 anti-mouse ABclonal Cat. # AS003; RRID: AB_2769851 anti-Myc ABclonal Cat. # AE010; RRID: AB_2770408 anti-rabbit ABclonal Cat. # AS014; RRID: AB_2769854 anti-Ser/Thr ECM Cat. # PM3801; RRID: AB_1944434 Bacterial and virus strains Agrobacterium tumefaciens strain GV3101 Weidi-Bio Cat. # AE1003 Escherichia coli BL21 (DE3) TransGen Biotech Cat. # CD801-02 A. Rhizobiaceae strain C58C1 Huayueyang Cat. # hyyd-10*100μl Escherichia coli Trans 5α TransGen Biotech Cat. # CD201-01 Biological samples Solanum lycopersicum: Leaf, root, stem, apical bud, lateral bud, fruit This study N/A Nicotiana benthamiana: Leaf This study N/A Chemicals, peptides, and recombinant proteins GFP-Trap magnetic beads Chromotek Cat. # AB_2631358 HA magnetic beads MCE Cat. # HY-K0201 Flag magnetic beads Merck Cat. #M8823-1ML Ni-NTA agarose Qiagen Cat. # 30210 Glutathione agarose Thermo Fisher Cat. # 16100 HBR MCE Cat. # HY-N9435 BRZ Sigma Cat. # SML1406 Bikinin MCE Cat. # HY12524 SD-Trp-Leu medium Coolaber Cat. # PM2222 SD-Ade-His-Leu-Trp medium Coolaber Cat. # PM2112 MG132 Abcam Cat. # ab141003 CHX MCE Cat. # HY-12320 BSA FD-Bio Cat. # FD0030 Critical commercial assays RNA prep pure Plant Kit Tiangen Cat. # DP419 ChamQ Universal SYBR qPCR Master Mix vazyme Cat. #Q711-02/03 HiScript II QRT SuperMix for qPCR vazyme Cat. #R223-01 ClonExpress® II One Step Cloning Kit vazyme Cat. #C112-01 FDbio-Femto Ecl Kit FD-Bio Cat. # FD8030 YeastmakerTM Yeast Transformation System 2 Clontech Cat. # 630439 Deposited data RNA-seq data This paper GSA accession: CRA033954 Experimental models: Organisms/strains S. lycopersicum: biw This paper N/A (Continued on next page) Cell Reports 45, 116876, February 24, 2026 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER S. lycopersicum: OE-BIW-HA This paper N/A S. lycopersicum: OE-BIWA-HA This paper N/A S. lycopersicum: biw/bzr1 This paper N/A S. lycopersicum: bzr1 Yin et al.77 N/A S. lycopersicum: OE-BZR1-HA Yin et al.77 N/A S. lycopersicum: OE-BIN2-HA An et al.28 N/A S. lycopersicum: dwf Li et al.53 N/A Oligonucleotides Primers used in this study This paper N/A Recombinant DNA AD-BIW This paper N/A BD-BIW This paper N/A BIW-HA This paper N/A BIWA-HA This paper N/A BIW-GFP This paper N/A BIWA-GFP This paper N/A BIWD-GFP This paper N/A BIW-Myc This paper N/A BIW-nYFP This paper N/A BIW-cYFP This paper N/A His-BIW This paper N/A AD-BZR1 This paper N/A AD-BZR1-N This paper N/A AD-BZR1-C This paper N/A BZR1-nYFP This paper N/A BZR1-cYFP This paper N/A BZR1-HA This paper N/A GST-BZR1-C This paper N/A BD-BIN2 This paper N/A BIN2-nYFP This paper N/A BIN2-cYFP This paper N/A GST-BIN2 This paper N/A BIN2-GFP This paper N/A UBQ-Flag This paper N/A DDB1-HA This paper N/A DDB1-GFP This paper N/A DDB1-nYFP This paper N/A CUL4-HA This paper N/A CUL4-GFP This paper N/A CUL4-nYFP This paper N/A BES1-nYFP This paper N/A BES1-cYFP This paper N/A D14-nYFP This paper N/A D14-cYFP This paper N/A Software and algorithms GraphPad 9.0 GraphPad Software https://www.graphpad.com ImageJ NIH https://imagej.net/ij/ Virus-induced gene silencing (VIGS) tool VIGS https://vigs.solgenomics.net/ 18 Cell Reports 45, 116876, February 24, 2026
Real-time Polymerase Chain Reaction:Article Title: The BZR1-interacting WD repeat protein participates in brassinosteroid-dependent growth and stress response in tomato.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-Actin ImmunoWay Cat. #YM3034 anti-Flag Invitrogen Cat. # FG4R; RRID: AB_1957945 anti-GFP Invitrogen Cat. # MA5-15256; RRID: AB_10979281 anti-GST ABclonal Cat. # AE001; RRID: AB_2770403 anti-HA Invitrogen Cat. # 26183; RRID: AB_10978021 anti-His ABclonal Cat. # AE003; RRID: AB_2728734 anti-mouse ABclonal Cat. # AS003; RRID: AB_2769851 anti-Myc ABclonal Cat. # AE010; RRID: AB_2770408 anti-rabbit ABclonal Cat. # AS014; RRID: AB_2769854 anti-Ser/Thr ECM Cat. # PM3801; RRID: AB_1944434 Bacterial and virus strains Agrobacterium tumefaciens strain GV3101 Weidi-Bio Cat. # AE1003 Escherichia coli BL21 (DE3) TransGen Biotech Cat. # CD801-02 A. Rhizobiaceae strain C58C1 Huayueyang Cat. # hyyd-10*100μl Escherichia coli Trans 5α TransGen Biotech Cat. # CD201-01 Biological samples Solanum lycopersicum: Leaf, root, stem, apical bud, lateral bud, fruit This study N/A Nicotiana benthamiana: Leaf This study N/A Chemicals, peptides, and recombinant proteins GFP-Trap magnetic beads Chromotek Cat. # AB_2631358 HA magnetic beads MCE Cat. # HY-K0201 Flag magnetic beads Merck Cat. #M8823-1ML Ni-NTA agarose Qiagen Cat. # 30210 Glutathione agarose Thermo Fisher Cat. # 16100 HBR MCE Cat. # HY-N9435 BRZ Sigma Cat. # SML1406 Bikinin MCE Cat. # HY12524 SD-Trp-Leu medium Coolaber Cat. # PM2222 SD-Ade-His-Leu-Trp medium Coolaber Cat. # PM2112 MG132 Abcam Cat. # ab141003 CHX MCE Cat. # HY-12320 BSA FD-Bio Cat. # FD0030 Critical commercial assays RNA prep pure Plant Kit Tiangen Cat. # DP419 ChamQ Universal SYBR qPCR Master Mix vazyme Cat. #Q711-02/03 HiScript II QRT SuperMix for qPCR vazyme Cat. #R223-01 ClonExpress® II One Step Cloning Kit vazyme Cat. #C112-01 FDbio-Femto Ecl Kit FD-Bio Cat. # FD8030 YeastmakerTM Yeast Transformation System 2 Clontech Cat. # 630439 Deposited data RNA-seq data This paper GSA accession: CRA033954 Experimental models: Organisms/strains S. lycopersicum: biw This paper N/A (Continued on next page) Cell Reports 45, 116876, February 24, 2026 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER S. lycopersicum: OE-BIW-HA This paper N/A S. lycopersicum: OE-BIWA-HA This paper N/A S. lycopersicum: biw/bzr1 This paper N/A S. lycopersicum: bzr1 Yin et al.77 N/A S. lycopersicum: OE-BZR1-HA Yin et al.77 N/A S. lycopersicum: OE-BIN2-HA An et al.28 N/A S. lycopersicum: dwf Li et al.53 N/A Oligonucleotides Primers used in this study This paper N/A Recombinant DNA AD-BIW This paper N/A BD-BIW This paper N/A BIW-HA This paper N/A BIWA-HA This paper N/A BIW-GFP This paper N/A BIWA-GFP This paper N/A BIWD-GFP This paper N/A BIW-Myc This paper N/A BIW-nYFP This paper N/A BIW-cYFP This paper N/A His-BIW This paper N/A AD-BZR1 This paper N/A AD-BZR1-N This paper N/A AD-BZR1-C This paper N/A BZR1-nYFP This paper N/A BZR1-cYFP This paper N/A BZR1-HA This paper N/A GST-BZR1-C This paper N/A BD-BIN2 This paper N/A BIN2-nYFP This paper N/A BIN2-cYFP This paper N/A GST-BIN2 This paper N/A BIN2-GFP This paper N/A UBQ-Flag This paper N/A DDB1-HA This paper N/A DDB1-GFP This paper N/A DDB1-nYFP This paper N/A CUL4-HA This paper N/A CUL4-GFP This paper N/A CUL4-nYFP This paper N/A BES1-nYFP This paper N/A BES1-cYFP This paper N/A D14-nYFP This paper N/A D14-cYFP This paper N/A Software and algorithms GraphPad 9.0 GraphPad Software https://www.graphpad.com ImageJ NIH https://imagej.net/ij/ Virus-induced gene silencing (VIGS) tool VIGS https://vigs.solgenomics.net/ 18 Cell Reports 45, 116876, February 24, 2026
Cloning:Article Title: The BZR1-interacting WD repeat protein participates in brassinosteroid-dependent growth and stress response in tomato.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-Actin ImmunoWay Cat. #YM3034 anti-Flag Invitrogen Cat. # FG4R; RRID: AB_1957945 anti-GFP Invitrogen Cat. # MA5-15256; RRID: AB_10979281 anti-GST ABclonal Cat. # AE001; RRID: AB_2770403 anti-HA Invitrogen Cat. # 26183; RRID: AB_10978021 anti-His ABclonal Cat. # AE003; RRID: AB_2728734 anti-mouse ABclonal Cat. # AS003; RRID: AB_2769851 anti-Myc ABclonal Cat. # AE010; RRID: AB_2770408 anti-rabbit ABclonal Cat. # AS014; RRID: AB_2769854 anti-Ser/Thr ECM Cat. # PM3801; RRID: AB_1944434 Bacterial and virus strains Agrobacterium tumefaciens strain GV3101 Weidi-Bio Cat. # AE1003 Escherichia coli BL21 (DE3) TransGen Biotech Cat. # CD801-02 A. Rhizobiaceae strain C58C1 Huayueyang Cat. # hyyd-10*100μl Escherichia coli Trans 5α TransGen Biotech Cat. # CD201-01 Biological samples Solanum lycopersicum: Leaf, root, stem, apical bud, lateral bud, fruit This study N/A Nicotiana benthamiana: Leaf This study N/A Chemicals, peptides, and recombinant proteins GFP-Trap magnetic beads Chromotek Cat. # AB_2631358 HA magnetic beads MCE Cat. # HY-K0201 Flag magnetic beads Merck Cat. #M8823-1ML Ni-NTA agarose Qiagen Cat. # 30210 Glutathione agarose Thermo Fisher Cat. # 16100 HBR MCE Cat. # HY-N9435 BRZ Sigma Cat. # SML1406 Bikinin MCE Cat. # HY12524 SD-Trp-Leu medium Coolaber Cat. # PM2222 SD-Ade-His-Leu-Trp medium Coolaber Cat. # PM2112 MG132 Abcam Cat. # ab141003 CHX MCE Cat. # HY-12320 BSA FD-Bio Cat. # FD0030 Critical commercial assays RNA prep pure Plant Kit Tiangen Cat. # DP419 ChamQ Universal SYBR qPCR Master Mix vazyme Cat. #Q711-02/03 HiScript II QRT SuperMix for qPCR vazyme Cat. #R223-01 ClonExpress® II One Step Cloning Kit vazyme Cat. #C112-01 FDbio-Femto Ecl Kit FD-Bio Cat. # FD8030 YeastmakerTM Yeast Transformation System 2 Clontech Cat. # 630439 Deposited data RNA-seq data This paper GSA accession: CRA033954 Experimental models: Organisms/strains S. lycopersicum: biw This paper N/A (Continued on next page) Cell Reports 45, 116876, February 24, 2026 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER S. lycopersicum: OE-BIW-HA This paper N/A S. lycopersicum: OE-BIWA-HA This paper N/A S. lycopersicum: biw/bzr1 This paper N/A S. lycopersicum: bzr1 Yin et al.77 N/A S. lycopersicum: OE-BZR1-HA Yin et al.77 N/A S. lycopersicum: OE-BIN2-HA An et al.28 N/A S. lycopersicum: dwf Li et al.53 N/A Oligonucleotides Primers used in this study This paper N/A Recombinant DNA AD-BIW This paper N/A BD-BIW This paper N/A BIW-HA This paper N/A BIWA-HA This paper N/A BIW-GFP This paper N/A BIWA-GFP This paper N/A BIWD-GFP This paper N/A BIW-Myc This paper N/A BIW-nYFP This paper N/A BIW-cYFP This paper N/A His-BIW This paper N/A AD-BZR1 This paper N/A AD-BZR1-N This paper N/A AD-BZR1-C This paper N/A BZR1-nYFP This paper N/A BZR1-cYFP This paper N/A BZR1-HA This paper N/A GST-BZR1-C This paper N/A BD-BIN2 This paper N/A BIN2-nYFP This paper N/A BIN2-cYFP This paper N/A GST-BIN2 This paper N/A BIN2-GFP This paper N/A UBQ-Flag This paper N/A DDB1-HA This paper N/A DDB1-GFP This paper N/A DDB1-nYFP This paper N/A CUL4-HA This paper N/A CUL4-GFP This paper N/A CUL4-nYFP This paper N/A BES1-nYFP This paper N/A BES1-cYFP This paper N/A D14-nYFP This paper N/A D14-cYFP This paper N/A Software and algorithms GraphPad 9.0 GraphPad Software https://www.graphpad.com ImageJ NIH https://imagej.net/ij/ Virus-induced gene silencing (VIGS) tool VIGS https://vigs.solgenomics.net/ 18 Cell Reports 45, 116876, February 24, 2026
Transformation Assay:Article Title: The BZR1-interacting WD repeat protein participates in brassinosteroid-dependent growth and stress response in tomato.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-Actin ImmunoWay Cat. #YM3034 anti-Flag Invitrogen Cat. # FG4R; RRID: AB_1957945 anti-GFP Invitrogen Cat. # MA5-15256; RRID: AB_10979281 anti-GST ABclonal Cat. # AE001; RRID: AB_2770403 anti-HA Invitrogen Cat. # 26183; RRID: AB_10978021 anti-His ABclonal Cat. # AE003; RRID: AB_2728734 anti-mouse ABclonal Cat. # AS003; RRID: AB_2769851 anti-Myc ABclonal Cat. # AE010; RRID: AB_2770408 anti-rabbit ABclonal Cat. # AS014; RRID: AB_2769854 anti-Ser/Thr ECM Cat. # PM3801; RRID: AB_1944434 Bacterial and virus strains Agrobacterium tumefaciens strain GV3101 Weidi-Bio Cat. # AE1003 Escherichia coli BL21 (DE3) TransGen Biotech Cat. # CD801-02 A. Rhizobiaceae strain C58C1 Huayueyang Cat. # hyyd-10*100μl Escherichia coli Trans 5α TransGen Biotech Cat. # CD201-01 Biological samples Solanum lycopersicum: Leaf, root, stem, apical bud, lateral bud, fruit This study N/A Nicotiana benthamiana: Leaf This study N/A Chemicals, peptides, and recombinant proteins GFP-Trap magnetic beads Chromotek Cat. # AB_2631358 HA magnetic beads MCE Cat. # HY-K0201 Flag magnetic beads Merck Cat. #M8823-1ML Ni-NTA agarose Qiagen Cat. # 30210 Glutathione agarose Thermo Fisher Cat. # 16100 HBR MCE Cat. # HY-N9435 BRZ Sigma Cat. # SML1406 Bikinin MCE Cat. # HY12524 SD-Trp-Leu medium Coolaber Cat. # PM2222 SD-Ade-His-Leu-Trp medium Coolaber Cat. # PM2112 MG132 Abcam Cat. # ab141003 CHX MCE Cat. # HY-12320 BSA FD-Bio Cat. # FD0030 Critical commercial assays RNA prep pure Plant Kit Tiangen Cat. # DP419 ChamQ Universal SYBR qPCR Master Mix vazyme Cat. #Q711-02/03 HiScript II QRT SuperMix for qPCR vazyme Cat. #R223-01 ClonExpress® II One Step Cloning Kit vazyme Cat. #C112-01 FDbio-Femto Ecl Kit FD-Bio Cat. # FD8030 YeastmakerTM Yeast Transformation System 2 Clontech Cat. # 630439 Deposited data RNA-seq data This paper GSA accession: CRA033954 Experimental models: Organisms/strains S. lycopersicum: biw This paper N/A (Continued on next page) Cell Reports 45, 116876, February 24, 2026 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER S. lycopersicum: OE-BIW-HA This paper N/A S. lycopersicum: OE-BIWA-HA This paper N/A S. lycopersicum: biw/bzr1 This paper N/A S. lycopersicum: bzr1 Yin et al.77 N/A S. lycopersicum: OE-BZR1-HA Yin et al.77 N/A S. lycopersicum: OE-BIN2-HA An et al.28 N/A S. lycopersicum: dwf Li et al.53 N/A Oligonucleotides Primers used in this study This paper N/A Recombinant DNA AD-BIW This paper N/A BD-BIW This paper N/A BIW-HA This paper N/A BIWA-HA This paper N/A BIW-GFP This paper N/A BIWA-GFP This paper N/A BIWD-GFP This paper N/A BIW-Myc This paper N/A BIW-nYFP This paper N/A BIW-cYFP This paper N/A His-BIW This paper N/A AD-BZR1 This paper N/A AD-BZR1-N This paper N/A AD-BZR1-C This paper N/A BZR1-nYFP This paper N/A BZR1-cYFP This paper N/A BZR1-HA This paper N/A GST-BZR1-C This paper N/A BD-BIN2 This paper N/A BIN2-nYFP This paper N/A BIN2-cYFP This paper N/A GST-BIN2 This paper N/A BIN2-GFP This paper N/A UBQ-Flag This paper N/A DDB1-HA This paper N/A DDB1-GFP This paper N/A DDB1-nYFP This paper N/A CUL4-HA This paper N/A CUL4-GFP This paper N/A CUL4-nYFP This paper N/A BES1-nYFP This paper N/A BES1-cYFP This paper N/A D14-nYFP This paper N/A D14-cYFP This paper N/A Software and algorithms GraphPad 9.0 GraphPad Software https://www.graphpad.com ImageJ NIH https://imagej.net/ij/ Virus-induced gene silencing (VIGS) tool VIGS https://vigs.solgenomics.net/ 18 Cell Reports 45, 116876, February 24, 2026
RNA Sequencing:Article Title: The BZR1-interacting WD repeat protein participates in brassinosteroid-dependent growth and stress response in tomato.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-Actin ImmunoWay Cat. #YM3034 anti-Flag Invitrogen Cat. # FG4R; RRID: AB_1957945 anti-GFP Invitrogen Cat. # MA5-15256; RRID: AB_10979281 anti-GST ABclonal Cat. # AE001; RRID: AB_2770403 anti-HA Invitrogen Cat. # 26183; RRID: AB_10978021 anti-His ABclonal Cat. # AE003; RRID: AB_2728734 anti-mouse ABclonal Cat. # AS003; RRID: AB_2769851 anti-Myc ABclonal Cat. # AE010; RRID: AB_2770408 anti-rabbit ABclonal Cat. # AS014; RRID: AB_2769854 anti-Ser/Thr ECM Cat. # PM3801; RRID: AB_1944434 Bacterial and virus strains Agrobacterium tumefaciens strain GV3101 Weidi-Bio Cat. # AE1003 Escherichia coli BL21 (DE3) TransGen Biotech Cat. # CD801-02 A. Rhizobiaceae strain C58C1 Huayueyang Cat. # hyyd-10*100μl Escherichia coli Trans 5α TransGen Biotech Cat. # CD201-01 Biological samples Solanum lycopersicum: Leaf, root, stem, apical bud, lateral bud, fruit This study N/A Nicotiana benthamiana: Leaf This study N/A Chemicals, peptides, and recombinant proteins GFP-Trap magnetic beads Chromotek Cat. # AB_2631358 HA magnetic beads MCE Cat. # HY-K0201 Flag magnetic beads Merck Cat. #M8823-1ML Ni-NTA agarose Qiagen Cat. # 30210 Glutathione agarose Thermo Fisher Cat. # 16100 HBR MCE Cat. # HY-N9435 BRZ Sigma Cat. # SML1406 Bikinin MCE Cat. # HY12524 SD-Trp-Leu medium Coolaber Cat. # PM2222 SD-Ade-His-Leu-Trp medium Coolaber Cat. # PM2112 MG132 Abcam Cat. # ab141003 CHX MCE Cat. # HY-12320 BSA FD-Bio Cat. # FD0030 Critical commercial assays RNA prep pure Plant Kit Tiangen Cat. # DP419 ChamQ Universal SYBR qPCR Master Mix vazyme Cat. #Q711-02/03 HiScript II QRT SuperMix for qPCR vazyme Cat. #R223-01 ClonExpress® II One Step Cloning Kit vazyme Cat. #C112-01 FDbio-Femto Ecl Kit FD-Bio Cat. # FD8030 YeastmakerTM Yeast Transformation System 2 Clontech Cat. # 630439 Deposited data RNA-seq data This paper GSA accession: CRA033954 Experimental models: Organisms/strains S. lycopersicum: biw This paper N/A (Continued on next page) Cell Reports 45, 116876, February 24, 2026 17 .. REAGENT or RESOURCE SOURCE IDENTIFIER S. lycopersicum: OE-BIW-HA This paper N/A S. lycopersicum: OE-BIWA-HA This paper N/A S. lycopersicum: biw/bzr1 This paper N/A S. lycopersicum: bzr1 Yin et al.77 N/A S. lycopersicum: OE-BZR1-HA Yin et al.77 N/A S. lycopersicum: OE-BIN2-HA An et al.28 N/A S. lycopersicum: dwf Li et al.53 N/A Oligonucleotides Primers used in this study This paper N/A Recombinant DNA AD-BIW This paper N/A BD-BIW This paper N/A BIW-HA This paper N/A BIWA-HA This paper N/A BIW-GFP This paper N/A BIWA-GFP This paper N/A BIWD-GFP This paper N/A BIW-Myc This paper N/A BIW-nYFP This paper N/A BIW-cYFP This paper N/A His-BIW This paper N/A AD-BZR1 This paper N/A AD-BZR1-N This paper N/A AD-BZR1-C This paper N/A BZR1-nYFP This paper N/A BZR1-cYFP This paper N/A BZR1-HA This paper N/A GST-BZR1-C This paper N/A BD-BIN2 This paper N/A BIN2-nYFP This paper N/A BIN2-cYFP This paper N/A GST-BIN2 This paper N/A BIN2-GFP This paper N/A UBQ-Flag This paper N/A DDB1-HA This paper N/A DDB1-GFP This paper N/A DDB1-nYFP This paper N/A CUL4-HA This paper N/A CUL4-GFP This paper N/A CUL4-nYFP This paper N/A BES1-nYFP This paper N/A BES1-cYFP This paper N/A D14-nYFP This paper N/A D14-cYFP This paper N/A Software and algorithms GraphPad 9.0 GraphPad Software https://www.graphpad.com ImageJ NIH https://imagej.net/ij/ Virus-induced gene silencing (VIGS) tool VIGS https://vigs.solgenomics.net/ 18 Cell Reports 45, 116876, February 24, 2026
other:Article Title: Vitazyme biostimulant increases specialty crop production and contains active brassinosteroids; a spectrofluorometric, chemical (LCMS), and bioassay determination.
Article Snippet: Reagents used were DAPBA, 95% purity (B148825, BenchChem), BL, > 99% purity (HY-N0273, MedChemExpress), and HBL, > 96% purity (HY-N9435, MedChemExpress).
Article Title: Vitazyme biostimulant increases specialty crop production and contains active brassinosteroids; a spectrofluorometric, chemical (LCMS), and bioassay determination
Article Snippet: Reagents used were DAPBA, 95% purity (B148825, BenchChem), BL, > 99% purity (HY-N0273, MedChemExpress), and HBL, > 96% purity (HY-N9435, MedChemExpress).
Article Title: Brassinosteroid signaling regulates PIN5/10-mediated intracellular auxin homeostasis to promote lateral root development in tomato.
Article Snippet: 578 579 Hormone and inhibitor treatments 580 The bioactive BR, 28-homobrassinolide (HBR, MCE, HY-N9435), was 581 dissolved in ethanol; indole-3-acetic acid (IAA, Sigma, 12886) in water; 582 brassinazole (BRZ, Sigma-Aldrich, SML1406), was dissolved in DMSO; and N-583 1-naphthylphthalamic acid (NPA, MCE, HY-18569) and 1-naphthaleneacetic 584 acid (NAA, MCE, HY-18570) in DMSO.
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