Protease Inhibitor:Article Title: Bile acids regulate lipid metabolism through selective actions on fatty acid absorption.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-CYP7A1 Novus Cat#NBP3-04836 lot 5500012145; RRID: AB_2255011 Rabbit polyclonal anti-CYP8B1 Abcam Cat#ab191910; RRID: AB_2828000 Rabbit polyclonal anti- rat CYP2C22 (mouse CYP2C70) Gift from Edward T. Morgan (Emory University) N/A Rabbit monoclonal anti-PDI Cell Signaling Technology Cat#3501S; RRID: AB_2156433 Rabbit monoclonal anti-ACC Cell Signaling Technology Cat#3676S; RRID: AV_2219397 Rabbit monoclonal anti-FASN Cell Signaling Technology Cat#3180S; RRID: AB_2100796 Rabbit monoclonal anti-SCD1 Cell Signaling Technology Cat#2794S; RRID: AB_2183099 Donkey polyclonal anti-Rabbit IgG Cytiva Cat# NA934-1ML; RRID: AB_772206 Mouse monoclonal anti-EpCAM (G8.8) DSHB Cat#G8.8; RRID: AB_2098655 Bacterial and virus strains pAAV-U6-SA-BbsI-MluI-gRNA-HLPSACas9-HA-OLLAS-spA This Paper vETV_0015 pAAV-U6-SA-mCyp7a1-gRNA6-HLPSACas9-HA-OLLAS-spA This Paper vETV_0028 pAAV-U6-SA-mCyp8b1-gRNA1-HLPSACas9-HA-OLLAS-spA This Paper vETV_0032 pAAV-U6-SA-mCyp2c70-gRNA4-HLPSACas9-HA-OLLAS-spA This Paper vETV_0033 pAAV-U6-SA-mCyp2a12-gRNA6-HLPSACas9-HA-OLLAS-spA This Paper vETV_0057 Biological samples Human Bile Human De-identified Chemicals, peptides, and recombinant proteins Sodium cholate Sigma-Aldrich Cat#C9282; Cas: 206986-87-0 Sodium taurocholate Sigma-Aldrich Cat#T4009; Cas: 345909-26-4 Chenodeoxycholic acid Sigma-Aldrich Cat#C9377; Cas: 474-25-9 Taurochenodeoxycholic acid (sodium salt) Cayman Chemical Cat#20275; Cas: 6009-98-9 Tauro-β-muricholic acid (sodium salt) Cayman Chemical Cat#20289; Cas: 145022-92-0 Sodium deoxycholate Sigma-Aldrich Cat#D6750; Cas: 302-95-4 Taurodeoxycholic acid, sodium salt Millipore Cat#580221; Cas: 1180-95-6 Ursodeoxycholic acid Sigma-Aldrich Cat#U5127; Cas: 128-13-2 Tauroursodeoxycholic acid, sodium salt Millipore Cat#580549; Cas: 35807-85-3 Sodium taurolithocholate Sigma-Aldrich Cat#T7515; Cas: 6042-32-6 Sodium glycocholate Chem-Impex Cat#27530; Cas: 863-57-0 Sodium glycochenodeoxycholate Chem-Impex Cat#21506; Cas: 16564-43-5 Glycodeoxycholic acid, sodium salt Chem-Impex Cat#21779; Cas: 16409-34-0 Glyco-chenodeoxycholic acid Sigma-Aldrich Cat#G0759; Cas: 16564-43-5 Aprotinin Sigma-Aldrich Cat# A6279; Cas: 9087-70-1 Dipeptidyl peptidase 4 (DPP-4) Sigma-Aldrich Cat# D38225MG LipidTOX Red ThermoFisher Cat#H34476 5α cholestane Sigma Aldrich Cat# C8003; Cas: 481-21-0 trinonadecanoin (C17:1) Nu-chek Prep Cat# T-165; Cas: 26536-13-0 cholesterol Sigma Aldrich Cat# C3045; Cas: 57-88-5 (Continued on next page) Cell Metabolism 38, 1–18.e1–e10, January 6, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER GLC96 Lipid Standards (mixed lipid standards) Nu-chek Prep Cat# GLC96 GLC96C Lipid Standards (mixed lipid standards) Nu-chek Prep Cat# GLC96C Deuterium oxide Sigma Aldrich Cat# 151882; Cas: 7789-20-0 Sucrose Polybehenate CarboMer Cas: 56449-50-4 coumarin 6 Sigma Aldrich Cat#442631; Cas: 38215-36-0 1,2-dioleoyl-sn-glycero-3-phosphocholine Avanti Polar Lipids Cat#850375; Cas: 4235-95-4 C18:0-d2 Cayman Cat#28150; Cas: 19905-58-9 C18:1-d17 Cayman Cat#9000432; Cas: 223487-44-3 C18:2-d11 Cayman Cat#9002193 GlutaMAX ThermoFisher Scientific Cat#35050061 N2 supplement Fisher Scientific Cat#17502-048 B27 supplement Fisher Scientific Cat#17504404 EGF Peprotech Cat#315-09; Cas: 62253-63-8 A83-01 Med Chem Express Cat#HY-10432; Cas: 909910-43-6 SB202190 Med Chem Express Cat#HY10295; Cas: 152121-03-7 Y-27632 Med Chem Express Cat#HY-10071; Cas: 146986-50-7 CHIR99021 Stem Cell Technologies Cat#72054; Cat: 252917-06-9 IGF-1 Med Chem Express Cat#HY-P70788 FGF2 Med Chem Express Cat#HY-P7004 Gastrin Med Chem Express Cat#HY-P2671 BMP-2 Med Chem Express Cat#HY-P7006 BMP-4 Med Chem Express Cat#HY-P7007 Orlistat TCI America Cat# O0381; Cas: 96829-58-2 Protease inhibitor cocktail tablets Med Chem Express Cat# HY-K0011 Phenylmethanesulfonyl fluoride Sigma Aldrich Cat#93482-50mL-F; Cas: 329-98-6 Calpain Inhibitor Sigma Aldrich Cat# A6185-25 mg; Cas: 110044-82-1 Critical commercial assays U-PLEX Custom Metabolic Group 1 Meso Scale Discovery Cat#K152ACM-1 Teco Diagnostics ALT Liquid Reagent Kinetic Method Kit Teco Diagnostics Cat# A524-150 DNeasy PowerSoil Pro Kit Qiagen Cat#47126 Qubit 1X dsDNA HS Assay Kit Invitrogen Cat#Q33231 Illumina DNA Prep workflow Invitrogen Cat#20060059 KAPA Pure Beads Roche Cat#07983271001 RNeasy mini kit Qiagen Cat#74104 KAPA RNA Hyperprep Kit KAPA Biosystems Cat# KK8541 KAPA Unique Duel-Indexed Adaptor Kit KAPA Biosystems Cat# KK8727 Bicinchoninic acid protein assay Fisher Cat#PI23227 Deposited data Microbiome Sequencing Data NCBI BioProject PRJNA1302405 Bulk Liver RNA Sequencing Data NCBI GEO GSE304800 Source Data This Paper Data S1 Experimental models: Cell lines HEK293T ATCC Cat#CRL-3216 De-identified human jejunal enteroids Dr. Martin Martin, UCLA N/A Experimental models: Organisms/strains Mouse: C57BL/6J The Jackson Laboratory Jax: 000664 (Continued on next page) e2 Cell Metabolism 38, 1–18.e1–e10, January 6, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Mouse: Srebp1c − /− (B6;129S6Srebf1 tm1Mbr /J The Jackson Laboratory Jax: 004365 Mouse: Gpr120 -/- Dr. Daisy Sahoo via Dr. Vincent Poitout 79 N/A Oligonucleotides Primers: See Table S4 N/A N/A Recombinant DNA 1313_pAAV-U6-BbsI-MluI-gRNA-SA-HLPSACas9-HA-OLLAS-spA Li et al. 80 Addgene Plasmid #109314 pETV_0065-U6-SA-mCyp7a1-gRNA6HLP-SACas9-HA-OLLAS-spA This Paper N/A pETV_0067-U6-SA-mCyp8b1-gRNA1HLP-SACas9-HA-OLLAS-spA This Paper N/A pETV_0073-U6-SA-mCyp2c70-gRNA4HLP-SACas9-HA-OLLAS-spA This Paper N/A pETV_0149-U6-SA-mCyp2a12-gRNA6HLP-SACas9-HA-OLLAS-spA This Paper N/A pAdDeltaF6 Puresyn Inc. Cat#PL-F-PVADF6 p0047 pAAV2/8 Puresyn Inc. Cat#PL-T-PV0007 p2123 Software and algorithms Graphpad Prism V10 Graphpad N/A Excel Microsoft N/A The R Project for Statistical Computing (v3.6.3 and v4.2.1) www.r-project.org N/A Bowtie2 https://bowtie-bio.sourceforge.net/ bowtie2/index.shtml 81,82 N/A BBDuk https://docs.seqera.io/multiqc/modules/ bbduk 83 N/A MetaPhlAn (v4.1.0) https://huttenhower.sph.harvard.edu/ metaphlan/ 84 N/A Vegan (v2.6.4) package https://cran.r-project.org/web/packages/ vegan/index.html N/A tidyverse (v2.0.0) https://tidyverse.org N/A ggplot2 (v3.5.0) https://ggplot2.tidyverse.org N/A cowplot (v1.1.3) https://cran.r-project.org/web/packages/ cowplot/vignettes/introduction.html N/A ggpubr (v0.6.0) https://rpkgs.datanovia.com/ggpubr/ N/A MaAsLin2 (v1.12.0) https://huttenhower.sph.harvard.edu/ maaslin/ 28 N/A Python (v3.6) Python N/A Custon Python TKinter for GC-MS analysis G. Calmettes: https://github.com/ gcalmettes/labUtils?tab=readme-ovfile#msanalyzer N/A Trim Galore https://github.com/FelixKrueger/ TrimGalore N/A STAR https://github.com/alexdobin/STAR 85 N/A Featurecounts Subread v2.0.6 https://subread.sourceforge.net 86 N/A DESeq2 (v1.48.1) https://bioconductor.org/packages/ release/bioc/html/DESeq2.html 36 N/A EnhancedVolcano (v1.16.0) https://bioconductor.org/packages/ release/bioc/html/EnhancedVolcano.html N/A RColorBrewer (v1.1.3) https://cran.r-project.org/web/packages/ RColorBrewer/index.html N/A (Continued on next page) Cell Metabolism 38, 1–18.e1–e10, January 6, 2026 e3
Bicinchoninic Acid Protein Assay:Article Title: Bile acids regulate lipid metabolism through selective actions on fatty acid absorption.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-CYP7A1 Novus Cat#NBP3-04836 lot 5500012145; RRID: AB_2255011 Rabbit polyclonal anti-CYP8B1 Abcam Cat#ab191910; RRID: AB_2828000 Rabbit polyclonal anti- rat CYP2C22 (mouse CYP2C70) Gift from Edward T. Morgan (Emory University) N/A Rabbit monoclonal anti-PDI Cell Signaling Technology Cat#3501S; RRID: AB_2156433 Rabbit monoclonal anti-ACC Cell Signaling Technology Cat#3676S; RRID: AV_2219397 Rabbit monoclonal anti-FASN Cell Signaling Technology Cat#3180S; RRID: AB_2100796 Rabbit monoclonal anti-SCD1 Cell Signaling Technology Cat#2794S; RRID: AB_2183099 Donkey polyclonal anti-Rabbit IgG Cytiva Cat# NA934-1ML; RRID: AB_772206 Mouse monoclonal anti-EpCAM (G8.8) DSHB Cat#G8.8; RRID: AB_2098655 Bacterial and virus strains pAAV-U6-SA-BbsI-MluI-gRNA-HLPSACas9-HA-OLLAS-spA This Paper vETV_0015 pAAV-U6-SA-mCyp7a1-gRNA6-HLPSACas9-HA-OLLAS-spA This Paper vETV_0028 pAAV-U6-SA-mCyp8b1-gRNA1-HLPSACas9-HA-OLLAS-spA This Paper vETV_0032 pAAV-U6-SA-mCyp2c70-gRNA4-HLPSACas9-HA-OLLAS-spA This Paper vETV_0033 pAAV-U6-SA-mCyp2a12-gRNA6-HLPSACas9-HA-OLLAS-spA This Paper vETV_0057 Biological samples Human Bile Human De-identified Chemicals, peptides, and recombinant proteins Sodium cholate Sigma-Aldrich Cat#C9282; Cas: 206986-87-0 Sodium taurocholate Sigma-Aldrich Cat#T4009; Cas: 345909-26-4 Chenodeoxycholic acid Sigma-Aldrich Cat#C9377; Cas: 474-25-9 Taurochenodeoxycholic acid (sodium salt) Cayman Chemical Cat#20275; Cas: 6009-98-9 Tauro-β-muricholic acid (sodium salt) Cayman Chemical Cat#20289; Cas: 145022-92-0 Sodium deoxycholate Sigma-Aldrich Cat#D6750; Cas: 302-95-4 Taurodeoxycholic acid, sodium salt Millipore Cat#580221; Cas: 1180-95-6 Ursodeoxycholic acid Sigma-Aldrich Cat#U5127; Cas: 128-13-2 Tauroursodeoxycholic acid, sodium salt Millipore Cat#580549; Cas: 35807-85-3 Sodium taurolithocholate Sigma-Aldrich Cat#T7515; Cas: 6042-32-6 Sodium glycocholate Chem-Impex Cat#27530; Cas: 863-57-0 Sodium glycochenodeoxycholate Chem-Impex Cat#21506; Cas: 16564-43-5 Glycodeoxycholic acid, sodium salt Chem-Impex Cat#21779; Cas: 16409-34-0 Glyco-chenodeoxycholic acid Sigma-Aldrich Cat#G0759; Cas: 16564-43-5 Aprotinin Sigma-Aldrich Cat# A6279; Cas: 9087-70-1 Dipeptidyl peptidase 4 (DPP-4) Sigma-Aldrich Cat# D38225MG LipidTOX Red ThermoFisher Cat#H34476 5α cholestane Sigma Aldrich Cat# C8003; Cas: 481-21-0 trinonadecanoin (C17:1) Nu-chek Prep Cat# T-165; Cas: 26536-13-0 cholesterol Sigma Aldrich Cat# C3045; Cas: 57-88-5 (Continued on next page) Cell Metabolism 38, 1–18.e1–e10, January 6, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER GLC96 Lipid Standards (mixed lipid standards) Nu-chek Prep Cat# GLC96 GLC96C Lipid Standards (mixed lipid standards) Nu-chek Prep Cat# GLC96C Deuterium oxide Sigma Aldrich Cat# 151882; Cas: 7789-20-0 Sucrose Polybehenate CarboMer Cas: 56449-50-4 coumarin 6 Sigma Aldrich Cat#442631; Cas: 38215-36-0 1,2-dioleoyl-sn-glycero-3-phosphocholine Avanti Polar Lipids Cat#850375; Cas: 4235-95-4 C18:0-d2 Cayman Cat#28150; Cas: 19905-58-9 C18:1-d17 Cayman Cat#9000432; Cas: 223487-44-3 C18:2-d11 Cayman Cat#9002193 GlutaMAX ThermoFisher Scientific Cat#35050061 N2 supplement Fisher Scientific Cat#17502-048 B27 supplement Fisher Scientific Cat#17504404 EGF Peprotech Cat#315-09; Cas: 62253-63-8 A83-01 Med Chem Express Cat#HY-10432; Cas: 909910-43-6 SB202190 Med Chem Express Cat#HY10295; Cas: 152121-03-7 Y-27632 Med Chem Express Cat#HY-10071; Cas: 146986-50-7 CHIR99021 Stem Cell Technologies Cat#72054; Cat: 252917-06-9 IGF-1 Med Chem Express Cat#HY-P70788 FGF2 Med Chem Express Cat#HY-P7004 Gastrin Med Chem Express Cat#HY-P2671 BMP-2 Med Chem Express Cat#HY-P7006 BMP-4 Med Chem Express Cat#HY-P7007 Orlistat TCI America Cat# O0381; Cas: 96829-58-2 Protease inhibitor cocktail tablets Med Chem Express Cat# HY-K0011 Phenylmethanesulfonyl fluoride Sigma Aldrich Cat#93482-50mL-F; Cas: 329-98-6 Calpain Inhibitor Sigma Aldrich Cat# A6185-25 mg; Cas: 110044-82-1 Critical commercial assays U-PLEX Custom Metabolic Group 1 Meso Scale Discovery Cat#K152ACM-1 Teco Diagnostics ALT Liquid Reagent Kinetic Method Kit Teco Diagnostics Cat# A524-150 DNeasy PowerSoil Pro Kit Qiagen Cat#47126 Qubit 1X dsDNA HS Assay Kit Invitrogen Cat#Q33231 Illumina DNA Prep workflow Invitrogen Cat#20060059 KAPA Pure Beads Roche Cat#07983271001 RNeasy mini kit Qiagen Cat#74104 KAPA RNA Hyperprep Kit KAPA Biosystems Cat# KK8541 KAPA Unique Duel-Indexed Adaptor Kit KAPA Biosystems Cat# KK8727 Bicinchoninic acid protein assay Fisher Cat#PI23227 Deposited data Microbiome Sequencing Data NCBI BioProject PRJNA1302405 Bulk Liver RNA Sequencing Data NCBI GEO GSE304800 Source Data This Paper Data S1 Experimental models: Cell lines HEK293T ATCC Cat#CRL-3216 De-identified human jejunal enteroids Dr. Martin Martin, UCLA N/A Experimental models: Organisms/strains Mouse: C57BL/6J The Jackson Laboratory Jax: 000664 (Continued on next page) e2 Cell Metabolism 38, 1–18.e1–e10, January 6, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Mouse: Srebp1c − /− (B6;129S6Srebf1 tm1Mbr /J The Jackson Laboratory Jax: 004365 Mouse: Gpr120 -/- Dr. Daisy Sahoo via Dr. Vincent Poitout 79 N/A Oligonucleotides Primers: See Table S4 N/A N/A Recombinant DNA 1313_pAAV-U6-BbsI-MluI-gRNA-SA-HLPSACas9-HA-OLLAS-spA Li et al. 80 Addgene Plasmid #109314 pETV_0065-U6-SA-mCyp7a1-gRNA6HLP-SACas9-HA-OLLAS-spA This Paper N/A pETV_0067-U6-SA-mCyp8b1-gRNA1HLP-SACas9-HA-OLLAS-spA This Paper N/A pETV_0073-U6-SA-mCyp2c70-gRNA4HLP-SACas9-HA-OLLAS-spA This Paper N/A pETV_0149-U6-SA-mCyp2a12-gRNA6HLP-SACas9-HA-OLLAS-spA This Paper N/A pAdDeltaF6 Puresyn Inc. Cat#PL-F-PVADF6 p0047 pAAV2/8 Puresyn Inc. Cat#PL-T-PV0007 p2123 Software and algorithms Graphpad Prism V10 Graphpad N/A Excel Microsoft N/A The R Project for Statistical Computing (v3.6.3 and v4.2.1) www.r-project.org N/A Bowtie2 https://bowtie-bio.sourceforge.net/ bowtie2/index.shtml 81,82 N/A BBDuk https://docs.seqera.io/multiqc/modules/ bbduk 83 N/A MetaPhlAn (v4.1.0) https://huttenhower.sph.harvard.edu/ metaphlan/ 84 N/A Vegan (v2.6.4) package https://cran.r-project.org/web/packages/ vegan/index.html N/A tidyverse (v2.0.0) https://tidyverse.org N/A ggplot2 (v3.5.0) https://ggplot2.tidyverse.org N/A cowplot (v1.1.3) https://cran.r-project.org/web/packages/ cowplot/vignettes/introduction.html N/A ggpubr (v0.6.0) https://rpkgs.datanovia.com/ggpubr/ N/A MaAsLin2 (v1.12.0) https://huttenhower.sph.harvard.edu/ maaslin/ 28 N/A Python (v3.6) Python N/A Custon Python TKinter for GC-MS analysis G. Calmettes: https://github.com/ gcalmettes/labUtils?tab=readme-ovfile#msanalyzer N/A Trim Galore https://github.com/FelixKrueger/ TrimGalore N/A STAR https://github.com/alexdobin/STAR 85 N/A Featurecounts Subread v2.0.6 https://subread.sourceforge.net 86 N/A DESeq2 (v1.48.1) https://bioconductor.org/packages/ release/bioc/html/DESeq2.html 36 N/A EnhancedVolcano (v1.16.0) https://bioconductor.org/packages/ release/bioc/html/EnhancedVolcano.html N/A RColorBrewer (v1.1.3) https://cran.r-project.org/web/packages/ RColorBrewer/index.html N/A (Continued on next page) Cell Metabolism 38, 1–18.e1–e10, January 6, 2026 e3
Sequencing:Article Title: Bile acids regulate lipid metabolism through selective actions on fatty acid absorption.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-CYP7A1 Novus Cat#NBP3-04836 lot 5500012145; RRID: AB_2255011 Rabbit polyclonal anti-CYP8B1 Abcam Cat#ab191910; RRID: AB_2828000 Rabbit polyclonal anti- rat CYP2C22 (mouse CYP2C70) Gift from Edward T. Morgan (Emory University) N/A Rabbit monoclonal anti-PDI Cell Signaling Technology Cat#3501S; RRID: AB_2156433 Rabbit monoclonal anti-ACC Cell Signaling Technology Cat#3676S; RRID: AV_2219397 Rabbit monoclonal anti-FASN Cell Signaling Technology Cat#3180S; RRID: AB_2100796 Rabbit monoclonal anti-SCD1 Cell Signaling Technology Cat#2794S; RRID: AB_2183099 Donkey polyclonal anti-Rabbit IgG Cytiva Cat# NA934-1ML; RRID: AB_772206 Mouse monoclonal anti-EpCAM (G8.8) DSHB Cat#G8.8; RRID: AB_2098655 Bacterial and virus strains pAAV-U6-SA-BbsI-MluI-gRNA-HLPSACas9-HA-OLLAS-spA This Paper vETV_0015 pAAV-U6-SA-mCyp7a1-gRNA6-HLPSACas9-HA-OLLAS-spA This Paper vETV_0028 pAAV-U6-SA-mCyp8b1-gRNA1-HLPSACas9-HA-OLLAS-spA This Paper vETV_0032 pAAV-U6-SA-mCyp2c70-gRNA4-HLPSACas9-HA-OLLAS-spA This Paper vETV_0033 pAAV-U6-SA-mCyp2a12-gRNA6-HLPSACas9-HA-OLLAS-spA This Paper vETV_0057 Biological samples Human Bile Human De-identified Chemicals, peptides, and recombinant proteins Sodium cholate Sigma-Aldrich Cat#C9282; Cas: 206986-87-0 Sodium taurocholate Sigma-Aldrich Cat#T4009; Cas: 345909-26-4 Chenodeoxycholic acid Sigma-Aldrich Cat#C9377; Cas: 474-25-9 Taurochenodeoxycholic acid (sodium salt) Cayman Chemical Cat#20275; Cas: 6009-98-9 Tauro-β-muricholic acid (sodium salt) Cayman Chemical Cat#20289; Cas: 145022-92-0 Sodium deoxycholate Sigma-Aldrich Cat#D6750; Cas: 302-95-4 Taurodeoxycholic acid, sodium salt Millipore Cat#580221; Cas: 1180-95-6 Ursodeoxycholic acid Sigma-Aldrich Cat#U5127; Cas: 128-13-2 Tauroursodeoxycholic acid, sodium salt Millipore Cat#580549; Cas: 35807-85-3 Sodium taurolithocholate Sigma-Aldrich Cat#T7515; Cas: 6042-32-6 Sodium glycocholate Chem-Impex Cat#27530; Cas: 863-57-0 Sodium glycochenodeoxycholate Chem-Impex Cat#21506; Cas: 16564-43-5 Glycodeoxycholic acid, sodium salt Chem-Impex Cat#21779; Cas: 16409-34-0 Glyco-chenodeoxycholic acid Sigma-Aldrich Cat#G0759; Cas: 16564-43-5 Aprotinin Sigma-Aldrich Cat# A6279; Cas: 9087-70-1 Dipeptidyl peptidase 4 (DPP-4) Sigma-Aldrich Cat# D38225MG LipidTOX Red ThermoFisher Cat#H34476 5α cholestane Sigma Aldrich Cat# C8003; Cas: 481-21-0 trinonadecanoin (C17:1) Nu-chek Prep Cat# T-165; Cas: 26536-13-0 cholesterol Sigma Aldrich Cat# C3045; Cas: 57-88-5 (Continued on next page) Cell Metabolism 38, 1–18.e1–e10, January 6, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER GLC96 Lipid Standards (mixed lipid standards) Nu-chek Prep Cat# GLC96 GLC96C Lipid Standards (mixed lipid standards) Nu-chek Prep Cat# GLC96C Deuterium oxide Sigma Aldrich Cat# 151882; Cas: 7789-20-0 Sucrose Polybehenate CarboMer Cas: 56449-50-4 coumarin 6 Sigma Aldrich Cat#442631; Cas: 38215-36-0 1,2-dioleoyl-sn-glycero-3-phosphocholine Avanti Polar Lipids Cat#850375; Cas: 4235-95-4 C18:0-d2 Cayman Cat#28150; Cas: 19905-58-9 C18:1-d17 Cayman Cat#9000432; Cas: 223487-44-3 C18:2-d11 Cayman Cat#9002193 GlutaMAX ThermoFisher Scientific Cat#35050061 N2 supplement Fisher Scientific Cat#17502-048 B27 supplement Fisher Scientific Cat#17504404 EGF Peprotech Cat#315-09; Cas: 62253-63-8 A83-01 Med Chem Express Cat#HY-10432; Cas: 909910-43-6 SB202190 Med Chem Express Cat#HY10295; Cas: 152121-03-7 Y-27632 Med Chem Express Cat#HY-10071; Cas: 146986-50-7 CHIR99021 Stem Cell Technologies Cat#72054; Cat: 252917-06-9 IGF-1 Med Chem Express Cat#HY-P70788 FGF2 Med Chem Express Cat#HY-P7004 Gastrin Med Chem Express Cat#HY-P2671 BMP-2 Med Chem Express Cat#HY-P7006 BMP-4 Med Chem Express Cat#HY-P7007 Orlistat TCI America Cat# O0381; Cas: 96829-58-2 Protease inhibitor cocktail tablets Med Chem Express Cat# HY-K0011 Phenylmethanesulfonyl fluoride Sigma Aldrich Cat#93482-50mL-F; Cas: 329-98-6 Calpain Inhibitor Sigma Aldrich Cat# A6185-25 mg; Cas: 110044-82-1 Critical commercial assays U-PLEX Custom Metabolic Group 1 Meso Scale Discovery Cat#K152ACM-1 Teco Diagnostics ALT Liquid Reagent Kinetic Method Kit Teco Diagnostics Cat# A524-150 DNeasy PowerSoil Pro Kit Qiagen Cat#47126 Qubit 1X dsDNA HS Assay Kit Invitrogen Cat#Q33231 Illumina DNA Prep workflow Invitrogen Cat#20060059 KAPA Pure Beads Roche Cat#07983271001 RNeasy mini kit Qiagen Cat#74104 KAPA RNA Hyperprep Kit KAPA Biosystems Cat# KK8541 KAPA Unique Duel-Indexed Adaptor Kit KAPA Biosystems Cat# KK8727 Bicinchoninic acid protein assay Fisher Cat#PI23227 Deposited data Microbiome Sequencing Data NCBI BioProject PRJNA1302405 Bulk Liver RNA Sequencing Data NCBI GEO GSE304800 Source Data This Paper Data S1 Experimental models: Cell lines HEK293T ATCC Cat#CRL-3216 De-identified human jejunal enteroids Dr. Martin Martin, UCLA N/A Experimental models: Organisms/strains Mouse: C57BL/6J The Jackson Laboratory Jax: 000664 (Continued on next page) e2 Cell Metabolism 38, 1–18.e1–e10, January 6, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Mouse: Srebp1c − /− (B6;129S6Srebf1 tm1Mbr /J The Jackson Laboratory Jax: 004365 Mouse: Gpr120 -/- Dr. Daisy Sahoo via Dr. Vincent Poitout 79 N/A Oligonucleotides Primers: See Table S4 N/A N/A Recombinant DNA 1313_pAAV-U6-BbsI-MluI-gRNA-SA-HLPSACas9-HA-OLLAS-spA Li et al. 80 Addgene Plasmid #109314 pETV_0065-U6-SA-mCyp7a1-gRNA6HLP-SACas9-HA-OLLAS-spA This Paper N/A pETV_0067-U6-SA-mCyp8b1-gRNA1HLP-SACas9-HA-OLLAS-spA This Paper N/A pETV_0073-U6-SA-mCyp2c70-gRNA4HLP-SACas9-HA-OLLAS-spA This Paper N/A pETV_0149-U6-SA-mCyp2a12-gRNA6HLP-SACas9-HA-OLLAS-spA This Paper N/A pAdDeltaF6 Puresyn Inc. Cat#PL-F-PVADF6 p0047 pAAV2/8 Puresyn Inc. Cat#PL-T-PV0007 p2123 Software and algorithms Graphpad Prism V10 Graphpad N/A Excel Microsoft N/A The R Project for Statistical Computing (v3.6.3 and v4.2.1) www.r-project.org N/A Bowtie2 https://bowtie-bio.sourceforge.net/ bowtie2/index.shtml 81,82 N/A BBDuk https://docs.seqera.io/multiqc/modules/ bbduk 83 N/A MetaPhlAn (v4.1.0) https://huttenhower.sph.harvard.edu/ metaphlan/ 84 N/A Vegan (v2.6.4) package https://cran.r-project.org/web/packages/ vegan/index.html N/A tidyverse (v2.0.0) https://tidyverse.org N/A ggplot2 (v3.5.0) https://ggplot2.tidyverse.org N/A cowplot (v1.1.3) https://cran.r-project.org/web/packages/ cowplot/vignettes/introduction.html N/A ggpubr (v0.6.0) https://rpkgs.datanovia.com/ggpubr/ N/A MaAsLin2 (v1.12.0) https://huttenhower.sph.harvard.edu/ maaslin/ 28 N/A Python (v3.6) Python N/A Custon Python TKinter for GC-MS analysis G. Calmettes: https://github.com/ gcalmettes/labUtils?tab=readme-ovfile#msanalyzer N/A Trim Galore https://github.com/FelixKrueger/ TrimGalore N/A STAR https://github.com/alexdobin/STAR 85 N/A Featurecounts Subread v2.0.6 https://subread.sourceforge.net 86 N/A DESeq2 (v1.48.1) https://bioconductor.org/packages/ release/bioc/html/DESeq2.html 36 N/A EnhancedVolcano (v1.16.0) https://bioconductor.org/packages/ release/bioc/html/EnhancedVolcano.html N/A RColorBrewer (v1.1.3) https://cran.r-project.org/web/packages/ RColorBrewer/index.html N/A (Continued on next page) Cell Metabolism 38, 1–18.e1–e10, January 6, 2026 e3
RNA Sequencing:Article Title: Bile acids regulate lipid metabolism through selective actions on fatty acid absorption.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-CYP7A1 Novus Cat#NBP3-04836 lot 5500012145; RRID: AB_2255011 Rabbit polyclonal anti-CYP8B1 Abcam Cat#ab191910; RRID: AB_2828000 Rabbit polyclonal anti- rat CYP2C22 (mouse CYP2C70) Gift from Edward T. Morgan (Emory University) N/A Rabbit monoclonal anti-PDI Cell Signaling Technology Cat#3501S; RRID: AB_2156433 Rabbit monoclonal anti-ACC Cell Signaling Technology Cat#3676S; RRID: AV_2219397 Rabbit monoclonal anti-FASN Cell Signaling Technology Cat#3180S; RRID: AB_2100796 Rabbit monoclonal anti-SCD1 Cell Signaling Technology Cat#2794S; RRID: AB_2183099 Donkey polyclonal anti-Rabbit IgG Cytiva Cat# NA934-1ML; RRID: AB_772206 Mouse monoclonal anti-EpCAM (G8.8) DSHB Cat#G8.8; RRID: AB_2098655 Bacterial and virus strains pAAV-U6-SA-BbsI-MluI-gRNA-HLPSACas9-HA-OLLAS-spA This Paper vETV_0015 pAAV-U6-SA-mCyp7a1-gRNA6-HLPSACas9-HA-OLLAS-spA This Paper vETV_0028 pAAV-U6-SA-mCyp8b1-gRNA1-HLPSACas9-HA-OLLAS-spA This Paper vETV_0032 pAAV-U6-SA-mCyp2c70-gRNA4-HLPSACas9-HA-OLLAS-spA This Paper vETV_0033 pAAV-U6-SA-mCyp2a12-gRNA6-HLPSACas9-HA-OLLAS-spA This Paper vETV_0057 Biological samples Human Bile Human De-identified Chemicals, peptides, and recombinant proteins Sodium cholate Sigma-Aldrich Cat#C9282; Cas: 206986-87-0 Sodium taurocholate Sigma-Aldrich Cat#T4009; Cas: 345909-26-4 Chenodeoxycholic acid Sigma-Aldrich Cat#C9377; Cas: 474-25-9 Taurochenodeoxycholic acid (sodium salt) Cayman Chemical Cat#20275; Cas: 6009-98-9 Tauro-β-muricholic acid (sodium salt) Cayman Chemical Cat#20289; Cas: 145022-92-0 Sodium deoxycholate Sigma-Aldrich Cat#D6750; Cas: 302-95-4 Taurodeoxycholic acid, sodium salt Millipore Cat#580221; Cas: 1180-95-6 Ursodeoxycholic acid Sigma-Aldrich Cat#U5127; Cas: 128-13-2 Tauroursodeoxycholic acid, sodium salt Millipore Cat#580549; Cas: 35807-85-3 Sodium taurolithocholate Sigma-Aldrich Cat#T7515; Cas: 6042-32-6 Sodium glycocholate Chem-Impex Cat#27530; Cas: 863-57-0 Sodium glycochenodeoxycholate Chem-Impex Cat#21506; Cas: 16564-43-5 Glycodeoxycholic acid, sodium salt Chem-Impex Cat#21779; Cas: 16409-34-0 Glyco-chenodeoxycholic acid Sigma-Aldrich Cat#G0759; Cas: 16564-43-5 Aprotinin Sigma-Aldrich Cat# A6279; Cas: 9087-70-1 Dipeptidyl peptidase 4 (DPP-4) Sigma-Aldrich Cat# D38225MG LipidTOX Red ThermoFisher Cat#H34476 5α cholestane Sigma Aldrich Cat# C8003; Cas: 481-21-0 trinonadecanoin (C17:1) Nu-chek Prep Cat# T-165; Cas: 26536-13-0 cholesterol Sigma Aldrich Cat# C3045; Cas: 57-88-5 (Continued on next page) Cell Metabolism 38, 1–18.e1–e10, January 6, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER GLC96 Lipid Standards (mixed lipid standards) Nu-chek Prep Cat# GLC96 GLC96C Lipid Standards (mixed lipid standards) Nu-chek Prep Cat# GLC96C Deuterium oxide Sigma Aldrich Cat# 151882; Cas: 7789-20-0 Sucrose Polybehenate CarboMer Cas: 56449-50-4 coumarin 6 Sigma Aldrich Cat#442631; Cas: 38215-36-0 1,2-dioleoyl-sn-glycero-3-phosphocholine Avanti Polar Lipids Cat#850375; Cas: 4235-95-4 C18:0-d2 Cayman Cat#28150; Cas: 19905-58-9 C18:1-d17 Cayman Cat#9000432; Cas: 223487-44-3 C18:2-d11 Cayman Cat#9002193 GlutaMAX ThermoFisher Scientific Cat#35050061 N2 supplement Fisher Scientific Cat#17502-048 B27 supplement Fisher Scientific Cat#17504404 EGF Peprotech Cat#315-09; Cas: 62253-63-8 A83-01 Med Chem Express Cat#HY-10432; Cas: 909910-43-6 SB202190 Med Chem Express Cat#HY10295; Cas: 152121-03-7 Y-27632 Med Chem Express Cat#HY-10071; Cas: 146986-50-7 CHIR99021 Stem Cell Technologies Cat#72054; Cat: 252917-06-9 IGF-1 Med Chem Express Cat#HY-P70788 FGF2 Med Chem Express Cat#HY-P7004 Gastrin Med Chem Express Cat#HY-P2671 BMP-2 Med Chem Express Cat#HY-P7006 BMP-4 Med Chem Express Cat#HY-P7007 Orlistat TCI America Cat# O0381; Cas: 96829-58-2 Protease inhibitor cocktail tablets Med Chem Express Cat# HY-K0011 Phenylmethanesulfonyl fluoride Sigma Aldrich Cat#93482-50mL-F; Cas: 329-98-6 Calpain Inhibitor Sigma Aldrich Cat# A6185-25 mg; Cas: 110044-82-1 Critical commercial assays U-PLEX Custom Metabolic Group 1 Meso Scale Discovery Cat#K152ACM-1 Teco Diagnostics ALT Liquid Reagent Kinetic Method Kit Teco Diagnostics Cat# A524-150 DNeasy PowerSoil Pro Kit Qiagen Cat#47126 Qubit 1X dsDNA HS Assay Kit Invitrogen Cat#Q33231 Illumina DNA Prep workflow Invitrogen Cat#20060059 KAPA Pure Beads Roche Cat#07983271001 RNeasy mini kit Qiagen Cat#74104 KAPA RNA Hyperprep Kit KAPA Biosystems Cat# KK8541 KAPA Unique Duel-Indexed Adaptor Kit KAPA Biosystems Cat# KK8727 Bicinchoninic acid protein assay Fisher Cat#PI23227 Deposited data Microbiome Sequencing Data NCBI BioProject PRJNA1302405 Bulk Liver RNA Sequencing Data NCBI GEO GSE304800 Source Data This Paper Data S1 Experimental models: Cell lines HEK293T ATCC Cat#CRL-3216 De-identified human jejunal enteroids Dr. Martin Martin, UCLA N/A Experimental models: Organisms/strains Mouse: C57BL/6J The Jackson Laboratory Jax: 000664 (Continued on next page) e2 Cell Metabolism 38, 1–18.e1–e10, January 6, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Mouse: Srebp1c − /− (B6;129S6Srebf1 tm1Mbr /J The Jackson Laboratory Jax: 004365 Mouse: Gpr120 -/- Dr. Daisy Sahoo via Dr. Vincent Poitout 79 N/A Oligonucleotides Primers: See Table S4 N/A N/A Recombinant DNA 1313_pAAV-U6-BbsI-MluI-gRNA-SA-HLPSACas9-HA-OLLAS-spA Li et al. 80 Addgene Plasmid #109314 pETV_0065-U6-SA-mCyp7a1-gRNA6HLP-SACas9-HA-OLLAS-spA This Paper N/A pETV_0067-U6-SA-mCyp8b1-gRNA1HLP-SACas9-HA-OLLAS-spA This Paper N/A pETV_0073-U6-SA-mCyp2c70-gRNA4HLP-SACas9-HA-OLLAS-spA This Paper N/A pETV_0149-U6-SA-mCyp2a12-gRNA6HLP-SACas9-HA-OLLAS-spA This Paper N/A pAdDeltaF6 Puresyn Inc. Cat#PL-F-PVADF6 p0047 pAAV2/8 Puresyn Inc. Cat#PL-T-PV0007 p2123 Software and algorithms Graphpad Prism V10 Graphpad N/A Excel Microsoft N/A The R Project for Statistical Computing (v3.6.3 and v4.2.1) www.r-project.org N/A Bowtie2 https://bowtie-bio.sourceforge.net/ bowtie2/index.shtml 81,82 N/A BBDuk https://docs.seqera.io/multiqc/modules/ bbduk 83 N/A MetaPhlAn (v4.1.0) https://huttenhower.sph.harvard.edu/ metaphlan/ 84 N/A Vegan (v2.6.4) package https://cran.r-project.org/web/packages/ vegan/index.html N/A tidyverse (v2.0.0) https://tidyverse.org N/A ggplot2 (v3.5.0) https://ggplot2.tidyverse.org N/A cowplot (v1.1.3) https://cran.r-project.org/web/packages/ cowplot/vignettes/introduction.html N/A ggpubr (v0.6.0) https://rpkgs.datanovia.com/ggpubr/ N/A MaAsLin2 (v1.12.0) https://huttenhower.sph.harvard.edu/ maaslin/ 28 N/A Python (v3.6) Python N/A Custon Python TKinter for GC-MS analysis G. Calmettes: https://github.com/ gcalmettes/labUtils?tab=readme-ovfile#msanalyzer N/A Trim Galore https://github.com/FelixKrueger/ TrimGalore N/A STAR https://github.com/alexdobin/STAR 85 N/A Featurecounts Subread v2.0.6 https://subread.sourceforge.net 86 N/A DESeq2 (v1.48.1) https://bioconductor.org/packages/ release/bioc/html/DESeq2.html 36 N/A EnhancedVolcano (v1.16.0) https://bioconductor.org/packages/ release/bioc/html/EnhancedVolcano.html N/A RColorBrewer (v1.1.3) https://cran.r-project.org/web/packages/ RColorBrewer/index.html N/A (Continued on next page) Cell Metabolism 38, 1–18.e1–e10, January 6, 2026 e3
Labeling:Article Title: Targeted Immune Modulation for Primary Biliary Cholangitis using Glycyrrhetinic Acid Liposomal Nanoparticles Loaded with Itaconic Acid
Article Snippet: .. The nanoparticles were labeled with Coumarin 6 (C6, HY-N7131, MedChemExpress, USA) and added to the cells for 3 and 6 hours of incubation. .. After incubation, cells were washed three times with cold PBS (pH 7.4) and fixed with 4% paraformaldehyde (PFA, P0099, Beyotime, Shanghai, China) for 15-20 minutes.
Incubation:Article Title: Targeted Immune Modulation for Primary Biliary Cholangitis using Glycyrrhetinic Acid Liposomal Nanoparticles Loaded with Itaconic Acid
Article Snippet: .. The nanoparticles were labeled with Coumarin 6 (C6, HY-N7131, MedChemExpress, USA) and added to the cells for 3 and 6 hours of incubation. .. After incubation, cells were washed three times with cold PBS (pH 7.4) and fixed with 4% paraformaldehyde (PFA, P0099, Beyotime, Shanghai, China) for 15-20 minutes.
|
MedChemExpress is a verified supplier 
MedChemExpress manufactures this product 