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<t>PAB</t> downregulates the expression of IAPs. ( A-B ) Western blotting analysis is performed on the lysates of A549 and H460 cells treated with PAB (0, 2.5, 5, 10 µM) for 24 h. ( C ) The changes in mitochondrial membrane potential (MMP) in A549 and H460 cells treated with PAB (0, 2.5, 5, 10 µM) for 24 h are detected. Red corresponds to the aggregate, and green corresponds to the monomerized. Scale bars, 50 μm. ( D ) The viability of A549 and H460 cells treated with PAB (10 µM) for 72 h in the presence or absence <t>of</t> <t>z-VAD-FMK</t> (10 µM) and CQ (20 µM) is determined by CCK8 assay. ( E ) Western blotting analysis is performed on the lysates of A549 and H460 cells treated with PAB (0, 2.5, 5, 10 µM) for 36 h. The results are presented as the means ± S .D., n ≥ 3, ** p < 0.01, *** p < 0.001 versus control group; ns, no significance.

Pab, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hy-n6939/Pseudolaric+Acid+B/pmc12966463-25-0-19
Average 94 stars, based on 3 article reviews

pab - by Bioz Stars, 2026-10

94/100 stars

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1) Product Images from "Pseudolaric acid B promotes lung cancer cells ferroptosis depending on JNK/ERK-mediated upregulation of survivin"

Article Title: Pseudolaric acid B promotes lung cancer cells ferroptosis depending on JNK/ERK-mediated upregulation of survivin

Journal: Scientific Reports

doi: 10.1038/s41598-026-36423-3

PAB downregulates the expression of IAPs. ( A-B ) Western blotting analysis is performed on the lysates of A549 and H460 cells treated with PAB (0, 2.5, 5, 10 µM) for 24 h. ( C ) The changes in mitochondrial membrane potential (MMP) in A549 and H460 cells treated with PAB (0, 2.5, 5, 10 µM) for 24 h are detected. Red corresponds to the aggregate, and green corresponds to the monomerized. Scale bars, 50 μm. ( D ) The viability of A549 and H460 cells treated with PAB (10 µM) for 72 h in the presence or absence of z-VAD-FMK (10 µM) and CQ (20 µM) is determined by CCK8 assay. ( E ) Western blotting analysis is performed on the lysates of A549 and H460 cells treated with PAB (0, 2.5, 5, 10 µM) for 36 h. The results are presented as the means ± S .D., n ≥ 3, ** p < 0.01, *** p < 0.001 versus control group; ns, no significance.

Figure Legend Snippet: PAB downregulates the expression of IAPs. ( A-B ) Western blotting analysis is performed on the lysates of A549 and H460 cells treated with PAB (0, 2.5, 5, 10 µM) for 24 h. ( C ) The changes in mitochondrial membrane potential (MMP) in A549 and H460 cells treated with PAB (0, 2.5, 5, 10 µM) for 24 h are detected. Red corresponds to the aggregate, and green corresponds to the monomerized. Scale bars, 50 μm. ( D ) The viability of A549 and H460 cells treated with PAB (10 µM) for 72 h in the presence or absence of z-VAD-FMK (10 µM) and CQ (20 µM) is determined by CCK8 assay. ( E ) Western blotting analysis is performed on the lysates of A549 and H460 cells treated with PAB (0, 2.5, 5, 10 µM) for 36 h. The results are presented as the means ± S .D., n ≥ 3, ** p < 0.01, *** p < 0.001 versus control group; ns, no significance.

Techniques Used: Expressing, Western Blot, Membrane, CCK-8 Assay, Control

PAB induces ferroptosis in lung cancer cells. ( A ) Volcano plots of differentially expressed genes. ( B ) The heat map mainly exhibits the expression levels of 10 up-regulated and 10 down-regulated differentially expressed genes (> 2.0-fold). ( C ) Representative transmission electron microscopy images show that the A549 cell is treated with PAB (10 µM) for 12 h. Scale bars, 2 μm. ( D ) Representative images of the impacts of PAB on mitochondrial morphology in A549 and H460 cells follow 12 h treated, with mitochondria labeled by Mito tracker. Scale bars, 5 μm. ( E ) The changes in mitochondrial reactive oxygen species in A549 and H460 cells treated with PAB (10 µM) for 0, 4, 8, 12 and 24 h are detected by mtSOX red probe. ( F , L ) The intracellular Fe 2+ in A549 and H460 cells are assessed after treatment with PAB (10 µM) either in the presence or absence of DFO (10 µM) for 24 h, followed by extraction for absorbance measurement at 550 nm. ( G , M ) Lipid peroxidation products are observed and statistically analyzed for 24 h after PAB (10 µM) stimulation of A549 and H460 cells in the presence or absence of DFO (10 µM). The red color indicated non-oxidized form while green color indicated oxidized form. Scale bar, 20 μm. ( H ) Western blotting analysis is performed on the lysates of A549 and H460 cells treated with PAB (0, 2.5, 5, 10 µM) for 36 h. ( I ) The viability of A549 and H460 cells treated with PAB (10 µM) for 72 h in the presence or absence of DFO (10 µM) is determined by CCK8 assay. ( J ) Cell viability of A549 and H460 cells following 72 h treatment with PAB (10 µM), either alone or in combination with DFO (10 µM), is assessed using crystal violet staining. ( K ) The quantitative analysis of cell apoptosis of A549 and H460 cells treated with PAB (10 µM) for 72 h in the presence or absence of DFO (10 µM) is determined. The results are presented as the means ± S .D., n ≥ 3, * p < 0.05, ** p < 0.01, *** p < 0.001 versus control group.

Figure Legend Snippet: PAB induces ferroptosis in lung cancer cells. ( A ) Volcano plots of differentially expressed genes. ( B ) The heat map mainly exhibits the expression levels of 10 up-regulated and 10 down-regulated differentially expressed genes (> 2.0-fold). ( C ) Representative transmission electron microscopy images show that the A549 cell is treated with PAB (10 µM) for 12 h. Scale bars, 2 μm. ( D ) Representative images of the impacts of PAB on mitochondrial morphology in A549 and H460 cells follow 12 h treated, with mitochondria labeled by Mito tracker. Scale bars, 5 μm. ( E ) The changes in mitochondrial reactive oxygen species in A549 and H460 cells treated with PAB (10 µM) for 0, 4, 8, 12 and 24 h are detected by mtSOX red probe. ( F , L ) The intracellular Fe 2+ in A549 and H460 cells are assessed after treatment with PAB (10 µM) either in the presence or absence of DFO (10 µM) for 24 h, followed by extraction for absorbance measurement at 550 nm. ( G , M ) Lipid peroxidation products are observed and statistically analyzed for 24 h after PAB (10 µM) stimulation of A549 and H460 cells in the presence or absence of DFO (10 µM). The red color indicated non-oxidized form while green color indicated oxidized form. Scale bar, 20 μm. ( H ) Western blotting analysis is performed on the lysates of A549 and H460 cells treated with PAB (0, 2.5, 5, 10 µM) for 36 h. ( I ) The viability of A549 and H460 cells treated with PAB (10 µM) for 72 h in the presence or absence of DFO (10 µM) is determined by CCK8 assay. ( J ) Cell viability of A549 and H460 cells following 72 h treatment with PAB (10 µM), either alone or in combination with DFO (10 µM), is assessed using crystal violet staining. ( K ) The quantitative analysis of cell apoptosis of A549 and H460 cells treated with PAB (10 µM) for 72 h in the presence or absence of DFO (10 µM) is determined. The results are presented as the means ± S .D., n ≥ 3, * p < 0.05, ** p < 0.01, *** p < 0.001 versus control group.

Techniques Used: Expressing, Transmission Assay, Electron Microscopy, Labeling, Extraction, Western Blot, CCK-8 Assay, Staining, Control

PAB-induced ferroptosis depends on upregulation of Survivin. ( A ) Western blotting analysis is conducted on the lysates of A549 and H460 cells treated with PAB (10 µM) for 48 h. ( B ) Western blotting analysis is conducted BIRC5 -knockdown A549 and H460 cells. ( C ) Crystal violet staining determines the viability of A549 and H460 cells after 72 h of treatment with PAB (10 µM) in the presence or absence of YM155 (10 µM). ( D ) The viability of A549 and H460 cells treated with PAB (10 µM) for 72 h in the presence or absence of YM155 (10 µM) is determined by CCK8 assay. ( E ) Analysis of cell viability in control and BIRC5 -knockdown A549 and H460 cells following treatment with PAB (10 µM) for 72 h by CCK8 assay. ( F ) The quantitative analysis of cell apoptosis of A549 and H460 cells treated with PAB (10 µM) for 72 h in the presence or absence of YM155 (10 µM) is determined. ( G ) The quantitative analysis of cell apoptosis in control and BIRC5 -knockdown A549 and H460 cells following treatment with PAB (10 µM) for 72 h. ( H ) The intracellular Fe 2+ in A549 and H460 cells are assessed after treatment with PAB (10 µM) either in the presence or absence of YM155 (10 µM) for 24 h, followed by extraction for absorbance measurement at 550 nm. ( I ) Lipid peroxidation products are observed and statistically analyzed for 24 h after PAB stimulation of A549 and H460 cells in the presence or absence of YM155 (10 µM). The red color indicated non-oxidized form and green color indicated oxidized form. Scale bar, 20 μm. ( J ) Western blotting analysis is performed on A549 and H460 cell lysates treated with PAB (10 µM) for 48 h in the presence or absence of YM155 (10 µM). ( K ) Western blotting analysis is performed and BIRC5 -knockdown A549 and H460 cells following treatment with PAB (10 µM) for 48 h. The results are presented as the means ± S .D., n ≥ 3, * p < 0.05, ** p < 0.01, *** p < 0.001 versus control group.

Figure Legend Snippet: PAB-induced ferroptosis depends on upregulation of Survivin. ( A ) Western blotting analysis is conducted on the lysates of A549 and H460 cells treated with PAB (10 µM) for 48 h. ( B ) Western blotting analysis is conducted BIRC5 -knockdown A549 and H460 cells. ( C ) Crystal violet staining determines the viability of A549 and H460 cells after 72 h of treatment with PAB (10 µM) in the presence or absence of YM155 (10 µM). ( D ) The viability of A549 and H460 cells treated with PAB (10 µM) for 72 h in the presence or absence of YM155 (10 µM) is determined by CCK8 assay. ( E ) Analysis of cell viability in control and BIRC5 -knockdown A549 and H460 cells following treatment with PAB (10 µM) for 72 h by CCK8 assay. ( F ) The quantitative analysis of cell apoptosis of A549 and H460 cells treated with PAB (10 µM) for 72 h in the presence or absence of YM155 (10 µM) is determined. ( G ) The quantitative analysis of cell apoptosis in control and BIRC5 -knockdown A549 and H460 cells following treatment with PAB (10 µM) for 72 h. ( H ) The intracellular Fe 2+ in A549 and H460 cells are assessed after treatment with PAB (10 µM) either in the presence or absence of YM155 (10 µM) for 24 h, followed by extraction for absorbance measurement at 550 nm. ( I ) Lipid peroxidation products are observed and statistically analyzed for 24 h after PAB stimulation of A549 and H460 cells in the presence or absence of YM155 (10 µM). The red color indicated non-oxidized form and green color indicated oxidized form. Scale bar, 20 μm. ( J ) Western blotting analysis is performed on A549 and H460 cell lysates treated with PAB (10 µM) for 48 h in the presence or absence of YM155 (10 µM). ( K ) Western blotting analysis is performed and BIRC5 -knockdown A549 and H460 cells following treatment with PAB (10 µM) for 48 h. The results are presented as the means ± S .D., n ≥ 3, * p < 0.05, ** p < 0.01, *** p < 0.001 versus control group.

Techniques Used: Western Blot, Knockdown, Staining, CCK-8 Assay, Control, Extraction

PAB-induced ferroptosis in lung cancer cells depends on JNK/ERK-mediated Survivin upregulation. ( A ) KEGG pathway analysis. ( B ) Western blotting analysis is conducted on the lysates of A549 and H460 cells treated with PAB (10 µM) for 0, 1, 2, 4, and 8 h. ( C ) The viability of A549 and H460 cells treated with PAB (10 µM) for 72 h in the presence or absence of SP600125 (10 µM) or U0126 (10 µM) is determined by CK8 assay. ( D ) Crystal violet staining is used to determine the viability of A549 and H460 cells after 72 h treatment with PAB (10 µM), either alone or in combination with SP600125 (10 µM) or U0126 (10 µM). ( E ) The quantitative analysis of cell apoptosis of A549 and H460 cells treated with PAB (10 µM) for 72 h in the presence or absence of SP600125 (10 µM) or U0126 (10 µM) is determined. ( F ) The intracellular Fe 2+ in A549 and H460 cells are assessed after treatment with PAB (10 µM) either in the presence or absence of SP600125 (10 µM) or U0126 (10 µM) for 24 h, followed by extraction for absorbance measurement at 550 nm. ( G ) Lipid peroxidation products are observed and statistically analyzed for 24 h after PAB (10 µM) stimulation of A549 and H460 cells in the presence or absence of SP600125 (10 µM) or U0126 (10 µM). The red color indicated non-oxidized form and green color indicated oxidized form. Scale bar, 20 μm. ( H-J ) Western blotting analyses are performed on A549 and H460 cell lysates treated with PAB (10 µM) for 48 h in the presence or absence of SP600125 (10 µM) or U0126 (10 µM). The results are presented as the means ± S .D., n ≥ 3, ** p ＜0.01, *** p < 0.001 versus control group.

Figure Legend Snippet: PAB-induced ferroptosis in lung cancer cells depends on JNK/ERK-mediated Survivin upregulation. ( A ) KEGG pathway analysis. ( B ) Western blotting analysis is conducted on the lysates of A549 and H460 cells treated with PAB (10 µM) for 0, 1, 2, 4, and 8 h. ( C ) The viability of A549 and H460 cells treated with PAB (10 µM) for 72 h in the presence or absence of SP600125 (10 µM) or U0126 (10 µM) is determined by CK8 assay. ( D ) Crystal violet staining is used to determine the viability of A549 and H460 cells after 72 h treatment with PAB (10 µM), either alone or in combination with SP600125 (10 µM) or U0126 (10 µM). ( E ) The quantitative analysis of cell apoptosis of A549 and H460 cells treated with PAB (10 µM) for 72 h in the presence or absence of SP600125 (10 µM) or U0126 (10 µM) is determined. ( F ) The intracellular Fe 2+ in A549 and H460 cells are assessed after treatment with PAB (10 µM) either in the presence or absence of SP600125 (10 µM) or U0126 (10 µM) for 24 h, followed by extraction for absorbance measurement at 550 nm. ( G ) Lipid peroxidation products are observed and statistically analyzed for 24 h after PAB (10 µM) stimulation of A549 and H460 cells in the presence or absence of SP600125 (10 µM) or U0126 (10 µM). The red color indicated non-oxidized form and green color indicated oxidized form. Scale bar, 20 μm. ( H-J ) Western blotting analyses are performed on A549 and H460 cell lysates treated with PAB (10 µM) for 48 h in the presence or absence of SP600125 (10 µM) or U0126 (10 µM). The results are presented as the means ± S .D., n ≥ 3, ** p ＜0.01, *** p < 0.001 versus control group.

Techniques Used: Western Blot, Staining, Extraction, Control

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Article Title: Pseudolaric Acid B Ameliorates Fungal Keratitis Progression by Suppressing Inflammation and Reducing Fungal Load.
Article Snippet: This study aimed to determine the mechanisms of antifungal and anti-inflammation effects of pseudolaric acid B (PAB) on Aspergillus fumigatus (A. fumigatus) keratitis.. In vitro MIC assay and crystal violet staining were conducted to evaluate the efficacy of PAB against A. fumigatus.. PAB inhibited A. fumigatus growth and inhibited the formation of fungal biofilms in a dosedependent manner.

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Journal: Scientific Reports

Article Title: Pseudolaric acid B promotes lung cancer cells ferroptosis depending on JNK/ERK-mediated upregulation of survivin

doi: 10.1038/s41598-026-36423-3

Figure Lengend Snippet: PAB downregulates the expression of IAPs. ( A-B ) Western blotting analysis is performed on the lysates of A549 and H460 cells treated with PAB (0, 2.5, 5, 10 µM) for 24 h. ( C ) The changes in mitochondrial membrane potential (MMP) in A549 and H460 cells treated with PAB (0, 2.5, 5, 10 µM) for 24 h are detected. Red corresponds to the aggregate, and green corresponds to the monomerized. Scale bars, 50 μm. ( D ) The viability of A549 and H460 cells treated with PAB (10 µM) for 72 h in the presence or absence of z-VAD-FMK (10 µM) and CQ (20 µM) is determined by CCK8 assay. ( E ) Western blotting analysis is performed on the lysates of A549 and H460 cells treated with PAB (0, 2.5, 5, 10 µM) for 36 h. The results are presented as the means ± S .D., n ≥ 3, ** p < 0.01, *** p < 0.001 versus control group; ns, no significance.

Article Snippet: PAB (HY-N6939), DFO (HY-B0568), SP600125 (HY-12041), U0126 (HY-12031 A), YM155 (HY-10194), z-VAD-FMK (HY-16658B), CQ (HY-17589 A) were purchased from MedChem Express (Shanghai, China).

Techniques: Expressing, Western Blot, Membrane, CCK-8 Assay, Control

Journal: Scientific Reports

Article Title: Pseudolaric acid B promotes lung cancer cells ferroptosis depending on JNK/ERK-mediated upregulation of survivin

doi: 10.1038/s41598-026-36423-3

Figure Lengend Snippet: PAB induces ferroptosis in lung cancer cells. ( A ) Volcano plots of differentially expressed genes. ( B ) The heat map mainly exhibits the expression levels of 10 up-regulated and 10 down-regulated differentially expressed genes (> 2.0-fold). ( C ) Representative transmission electron microscopy images show that the A549 cell is treated with PAB (10 µM) for 12 h. Scale bars, 2 μm. ( D ) Representative images of the impacts of PAB on mitochondrial morphology in A549 and H460 cells follow 12 h treated, with mitochondria labeled by Mito tracker. Scale bars, 5 μm. ( E ) The changes in mitochondrial reactive oxygen species in A549 and H460 cells treated with PAB (10 µM) for 0, 4, 8, 12 and 24 h are detected by mtSOX red probe. ( F , L ) The intracellular Fe 2+ in A549 and H460 cells are assessed after treatment with PAB (10 µM) either in the presence or absence of DFO (10 µM) for 24 h, followed by extraction for absorbance measurement at 550 nm. ( G , M ) Lipid peroxidation products are observed and statistically analyzed for 24 h after PAB (10 µM) stimulation of A549 and H460 cells in the presence or absence of DFO (10 µM). The red color indicated non-oxidized form while green color indicated oxidized form. Scale bar, 20 μm. ( H ) Western blotting analysis is performed on the lysates of A549 and H460 cells treated with PAB (0, 2.5, 5, 10 µM) for 36 h. ( I ) The viability of A549 and H460 cells treated with PAB (10 µM) for 72 h in the presence or absence of DFO (10 µM) is determined by CCK8 assay. ( J ) Cell viability of A549 and H460 cells following 72 h treatment with PAB (10 µM), either alone or in combination with DFO (10 µM), is assessed using crystal violet staining. ( K ) The quantitative analysis of cell apoptosis of A549 and H460 cells treated with PAB (10 µM) for 72 h in the presence or absence of DFO (10 µM) is determined. The results are presented as the means ± S .D., n ≥ 3, * p < 0.05, ** p < 0.01, *** p < 0.001 versus control group.

Techniques: Expressing, Transmission Assay, Electron Microscopy, Labeling, Extraction, Western Blot, CCK-8 Assay, Staining, Control

Journal: Scientific Reports

Article Title: Pseudolaric acid B promotes lung cancer cells ferroptosis depending on JNK/ERK-mediated upregulation of survivin

doi: 10.1038/s41598-026-36423-3

Figure Lengend Snippet: PAB-induced ferroptosis depends on upregulation of Survivin. ( A ) Western blotting analysis is conducted on the lysates of A549 and H460 cells treated with PAB (10 µM) for 48 h. ( B ) Western blotting analysis is conducted BIRC5 -knockdown A549 and H460 cells. ( C ) Crystal violet staining determines the viability of A549 and H460 cells after 72 h of treatment with PAB (10 µM) in the presence or absence of YM155 (10 µM). ( D ) The viability of A549 and H460 cells treated with PAB (10 µM) for 72 h in the presence or absence of YM155 (10 µM) is determined by CCK8 assay. ( E ) Analysis of cell viability in control and BIRC5 -knockdown A549 and H460 cells following treatment with PAB (10 µM) for 72 h by CCK8 assay. ( F ) The quantitative analysis of cell apoptosis of A549 and H460 cells treated with PAB (10 µM) for 72 h in the presence or absence of YM155 (10 µM) is determined. ( G ) The quantitative analysis of cell apoptosis in control and BIRC5 -knockdown A549 and H460 cells following treatment with PAB (10 µM) for 72 h. ( H ) The intracellular Fe 2+ in A549 and H460 cells are assessed after treatment with PAB (10 µM) either in the presence or absence of YM155 (10 µM) for 24 h, followed by extraction for absorbance measurement at 550 nm. ( I ) Lipid peroxidation products are observed and statistically analyzed for 24 h after PAB stimulation of A549 and H460 cells in the presence or absence of YM155 (10 µM). The red color indicated non-oxidized form and green color indicated oxidized form. Scale bar, 20 μm. ( J ) Western blotting analysis is performed on A549 and H460 cell lysates treated with PAB (10 µM) for 48 h in the presence or absence of YM155 (10 µM). ( K ) Western blotting analysis is performed and BIRC5 -knockdown A549 and H460 cells following treatment with PAB (10 µM) for 48 h. The results are presented as the means ± S .D., n ≥ 3, * p < 0.05, ** p < 0.01, *** p < 0.001 versus control group.

Techniques: Western Blot, Knockdown, Staining, CCK-8 Assay, Control, Extraction

Journal: Scientific Reports

Article Title: Pseudolaric acid B promotes lung cancer cells ferroptosis depending on JNK/ERK-mediated upregulation of survivin

doi: 10.1038/s41598-026-36423-3

Figure Lengend Snippet: PAB-induced ferroptosis in lung cancer cells depends on JNK/ERK-mediated Survivin upregulation. ( A ) KEGG pathway analysis. ( B ) Western blotting analysis is conducted on the lysates of A549 and H460 cells treated with PAB (10 µM) for 0, 1, 2, 4, and 8 h. ( C ) The viability of A549 and H460 cells treated with PAB (10 µM) for 72 h in the presence or absence of SP600125 (10 µM) or U0126 (10 µM) is determined by CK8 assay. ( D ) Crystal violet staining is used to determine the viability of A549 and H460 cells after 72 h treatment with PAB (10 µM), either alone or in combination with SP600125 (10 µM) or U0126 (10 µM). ( E ) The quantitative analysis of cell apoptosis of A549 and H460 cells treated with PAB (10 µM) for 72 h in the presence or absence of SP600125 (10 µM) or U0126 (10 µM) is determined. ( F ) The intracellular Fe 2+ in A549 and H460 cells are assessed after treatment with PAB (10 µM) either in the presence or absence of SP600125 (10 µM) or U0126 (10 µM) for 24 h, followed by extraction for absorbance measurement at 550 nm. ( G ) Lipid peroxidation products are observed and statistically analyzed for 24 h after PAB (10 µM) stimulation of A549 and H460 cells in the presence or absence of SP600125 (10 µM) or U0126 (10 µM). The red color indicated non-oxidized form and green color indicated oxidized form. Scale bar, 20 μm. ( H-J ) Western blotting analyses are performed on A549 and H460 cell lysates treated with PAB (10 µM) for 48 h in the presence or absence of SP600125 (10 µM) or U0126 (10 µM). The results are presented as the means ± S .D., n ≥ 3, ** p ＜0.01, *** p < 0.001 versus control group.

Techniques: Western Blot, Staining, Extraction, Control

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pab (MedChemExpress)

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1) Product Images from "Pseudolaric acid B promotes lung cancer cells ferroptosis depending on JNK/ERK-mediated upregulation of survivin"

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