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crocetin monomethyl ester  (MedChemExpress)


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    Structured Review

    MedChemExpress crocetin monomethyl ester
    Crocetin Monomethyl Ester, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hy-n6904/Crocetin+monomethyl+ester/pmc12749825-343-115-126
    Average 93 stars, based on 1 article reviews
    crocetin monomethyl ester - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Purification:

    Article Title: Dcaf15-mediated EphA2 degradation triggers disruption of the blood-brain barrier during Streptococcus suis meningitis.
    Article Snippet: .. 500 Antibodies and reagents 501 The following antibodies were used in this study: anti-EphA2 (6997), anti-LC3B 502 (3868T), anti-AKT (9272S), anti-Phospho-AKT (Ser473) (4060S), anti-Phospho-503 p44/42 MAPK (ERK1/2) (Thr202/Tyr204) (4370T), anti-p44/42MAPK (ERK1/2) 504 (4695T), anti-AMPKα (5831), Anti-Phospho-AMPKα (Thr172) (2535) and Phospho-505 LKB1 (Ser334) (3055) antibodies were from Cell Signaling Technology; anti-506 DYKDDDDK tag (20543-1-AP), anti-P62/SQSTM1 (18420-1-AP), anti-507 STK11/LKB1 (10746-1-AP), anti-ZO-1 (21773-1-AP), anti-SIRT1 (60303-1-Ig), anti-508 HA tag polyclonal antibody (51064-2-AP), anti-Myc (16286-1-AP), anti-509 AR TI CL E IN P RE SS DYKDDDDK (66008-4-Ig) and histone H3 (17168-1-AP) antibodies were from 510 protein tech; Anti-Phospho-(Ser/Thr) (ab117253) and anti-P300 (ab275378) antibodies 511 were from Abcam; anti-Ubiquitin ( sc-8017) antibody was from santa cruz; anti-Acetyl 512 lysine antibody (PTM-105RM) was from PTM BIO; DCAF15 antibody was from 513 Sigma; 3-methyladenine (3MA, HY-19312), Rapamycin (Rapa, HY-10219), MG-132 514 (HY-13259), Chloroquine (CQ, HY-17589A ), Pim1/AKK1-IN-1 (Pim1, HY-10371), 515 Crocetin monomethyl ester (HY-N6904), cycloheximide (HY-12320) and DMSO (HY-516 Y0320) were from MCE; Anti-Myc affinity gel (P2285), Protein A+G agarose (P2055), 517 GST-tag purification resin (P2251) and NP-40(P0013F) were from Beyotime; Anti-518 DYKDDDDK G1 affinity resin (L00432) was from Gen Script, Lipomaster 293 519 transfection reagent (JC3001) and ChamQ Blue Universal SYBR qPCR Master Mix 520 (Q312) were from Vazyme; LipofectamineTM 3000 (L3000008) was from Thermo 521 Scientific; SIRT1 Fusion Protein (Ag17677) and STK11 Fusion Protein (Ag1048) were 522 from protein tech. .. 523 Plasmid constructs and transfection 524 The plasmids including Flag-tagged DCAF15-K562R, DCAF15-K560R, 525 DCAF15-K581R, DCAF15-2KR, DCAF15-2KQ acquired from BGI (Beijing, China).

    Article Title: Dcaf15-mediated EphA2 degradation triggers disruption of the blood-brain barrier during Streptococcus suis meningitis
    Article Snippet: .. The following antibodies were used in this study: anti-EphA2 (6997), anti-LC3B (3868 T), anti-AKT (9272S), anti-Phospho-AKT (Ser473) (4060S), anti-Phospho-p44/42 MAPK (ERK1/2) (Thr202/Tyr204) (4370 T), anti-p44/42MAPK (ERK1/2) (4695 T), anti-AMPKα (5831), Anti-Phospho-AMPKα (Thr172) (2535) and Phospho-LKB1 (Ser334) (3055) antibodies were from Cell Signaling Technology; anti-DYKDDDDK tag (20543-1-AP), anti-P62/SQSTM1 (18420-1-AP), anti-STK11/LKB1 (10746-1-AP), anti-ZO-1 (21773-1-AP), anti-SIRT1 (60303-1-Ig), anti-HA tag polyclonal antibody (51064-2-AP), anti-Myc (16286-1-AP), anti-DYKDDDDK (66008-4-Ig) and histone H3 (17168-1-AP) antibodies were from protein tech; Anti-Phospho-(Ser/Thr) (ab117253) and anti-P300 (ab275378) antibodies were from Abcam; anti-Ubiquitin (sc-8017) antibody was from santa cruz; anti-Acetyl lysine antibody (PTM-105RM) was from PTM BIO; DCAF15 antibody was from Sigma; 3-methyladenine (3MA, HY-19312), Rapamycin (Rapa, HY-10219), MG-132 (HY-13259), Chloroquine (CQ, HY-17589A), Pim1/AKK1-IN-1 (Pim1, HY-10371), Crocetin monomethyl ester (HY-N6904), cycloheximide (HY-12320) and DMSO (HY-Y0320) were from MCE; Anti-Myc affinity gel (P2285), Protein A + G agarose (P2055), GST-tag purification resin (P2251) and NP-40(P0013F) were from Beyotime; Anti-DYKDDDDK G1 affinity resin (L00432) was from Gen Script, Lipomaster 293 transfection reagent (JC3001) and ChamQ Blue Universal SYBR qPCR Master Mix (Q312) were from Vazyme; LipofectamineTM 3000 (L3000008) was from Thermo Scientific; SIRT1 Fusion Protein (Ag17677) and STK11 Fusion Protein (Ag1048) were from protein tech. .. The plasmids, including Flag-tagged DCAF15-K562R, DCAF15-K560R, DCAF15-K581R, DCAF15-2KR, DCAF15-2KQ acquired from BGI (Beijing, China).

    Transfection:

    Article Title: Dcaf15-mediated EphA2 degradation triggers disruption of the blood-brain barrier during Streptococcus suis meningitis.
    Article Snippet: .. 500 Antibodies and reagents 501 The following antibodies were used in this study: anti-EphA2 (6997), anti-LC3B 502 (3868T), anti-AKT (9272S), anti-Phospho-AKT (Ser473) (4060S), anti-Phospho-503 p44/42 MAPK (ERK1/2) (Thr202/Tyr204) (4370T), anti-p44/42MAPK (ERK1/2) 504 (4695T), anti-AMPKα (5831), Anti-Phospho-AMPKα (Thr172) (2535) and Phospho-505 LKB1 (Ser334) (3055) antibodies were from Cell Signaling Technology; anti-506 DYKDDDDK tag (20543-1-AP), anti-P62/SQSTM1 (18420-1-AP), anti-507 STK11/LKB1 (10746-1-AP), anti-ZO-1 (21773-1-AP), anti-SIRT1 (60303-1-Ig), anti-508 HA tag polyclonal antibody (51064-2-AP), anti-Myc (16286-1-AP), anti-509 AR TI CL E IN P RE SS DYKDDDDK (66008-4-Ig) and histone H3 (17168-1-AP) antibodies were from 510 protein tech; Anti-Phospho-(Ser/Thr) (ab117253) and anti-P300 (ab275378) antibodies 511 were from Abcam; anti-Ubiquitin ( sc-8017) antibody was from santa cruz; anti-Acetyl 512 lysine antibody (PTM-105RM) was from PTM BIO; DCAF15 antibody was from 513 Sigma; 3-methyladenine (3MA, HY-19312), Rapamycin (Rapa, HY-10219), MG-132 514 (HY-13259), Chloroquine (CQ, HY-17589A ), Pim1/AKK1-IN-1 (Pim1, HY-10371), 515 Crocetin monomethyl ester (HY-N6904), cycloheximide (HY-12320) and DMSO (HY-516 Y0320) were from MCE; Anti-Myc affinity gel (P2285), Protein A+G agarose (P2055), 517 GST-tag purification resin (P2251) and NP-40(P0013F) were from Beyotime; Anti-518 DYKDDDDK G1 affinity resin (L00432) was from Gen Script, Lipomaster 293 519 transfection reagent (JC3001) and ChamQ Blue Universal SYBR qPCR Master Mix 520 (Q312) were from Vazyme; LipofectamineTM 3000 (L3000008) was from Thermo 521 Scientific; SIRT1 Fusion Protein (Ag17677) and STK11 Fusion Protein (Ag1048) were 522 from protein tech. .. 523 Plasmid constructs and transfection 524 The plasmids including Flag-tagged DCAF15-K562R, DCAF15-K560R, 525 DCAF15-K581R, DCAF15-2KR, DCAF15-2KQ acquired from BGI (Beijing, China).

    Article Title: Dcaf15-mediated EphA2 degradation triggers disruption of the blood-brain barrier during Streptococcus suis meningitis
    Article Snippet: .. The following antibodies were used in this study: anti-EphA2 (6997), anti-LC3B (3868 T), anti-AKT (9272S), anti-Phospho-AKT (Ser473) (4060S), anti-Phospho-p44/42 MAPK (ERK1/2) (Thr202/Tyr204) (4370 T), anti-p44/42MAPK (ERK1/2) (4695 T), anti-AMPKα (5831), Anti-Phospho-AMPKα (Thr172) (2535) and Phospho-LKB1 (Ser334) (3055) antibodies were from Cell Signaling Technology; anti-DYKDDDDK tag (20543-1-AP), anti-P62/SQSTM1 (18420-1-AP), anti-STK11/LKB1 (10746-1-AP), anti-ZO-1 (21773-1-AP), anti-SIRT1 (60303-1-Ig), anti-HA tag polyclonal antibody (51064-2-AP), anti-Myc (16286-1-AP), anti-DYKDDDDK (66008-4-Ig) and histone H3 (17168-1-AP) antibodies were from protein tech; Anti-Phospho-(Ser/Thr) (ab117253) and anti-P300 (ab275378) antibodies were from Abcam; anti-Ubiquitin (sc-8017) antibody was from santa cruz; anti-Acetyl lysine antibody (PTM-105RM) was from PTM BIO; DCAF15 antibody was from Sigma; 3-methyladenine (3MA, HY-19312), Rapamycin (Rapa, HY-10219), MG-132 (HY-13259), Chloroquine (CQ, HY-17589A), Pim1/AKK1-IN-1 (Pim1, HY-10371), Crocetin monomethyl ester (HY-N6904), cycloheximide (HY-12320) and DMSO (HY-Y0320) were from MCE; Anti-Myc affinity gel (P2285), Protein A + G agarose (P2055), GST-tag purification resin (P2251) and NP-40(P0013F) were from Beyotime; Anti-DYKDDDDK G1 affinity resin (L00432) was from Gen Script, Lipomaster 293 transfection reagent (JC3001) and ChamQ Blue Universal SYBR qPCR Master Mix (Q312) were from Vazyme; LipofectamineTM 3000 (L3000008) was from Thermo Scientific; SIRT1 Fusion Protein (Ag17677) and STK11 Fusion Protein (Ag1048) were from protein tech. .. The plasmids, including Flag-tagged DCAF15-K562R, DCAF15-K560R, DCAF15-K581R, DCAF15-2KR, DCAF15-2KQ acquired from BGI (Beijing, China).

    Real-time Polymerase Chain Reaction:

    Article Title: Dcaf15-mediated EphA2 degradation triggers disruption of the blood-brain barrier during Streptococcus suis meningitis.
    Article Snippet: .. 500 Antibodies and reagents 501 The following antibodies were used in this study: anti-EphA2 (6997), anti-LC3B 502 (3868T), anti-AKT (9272S), anti-Phospho-AKT (Ser473) (4060S), anti-Phospho-503 p44/42 MAPK (ERK1/2) (Thr202/Tyr204) (4370T), anti-p44/42MAPK (ERK1/2) 504 (4695T), anti-AMPKα (5831), Anti-Phospho-AMPKα (Thr172) (2535) and Phospho-505 LKB1 (Ser334) (3055) antibodies were from Cell Signaling Technology; anti-506 DYKDDDDK tag (20543-1-AP), anti-P62/SQSTM1 (18420-1-AP), anti-507 STK11/LKB1 (10746-1-AP), anti-ZO-1 (21773-1-AP), anti-SIRT1 (60303-1-Ig), anti-508 HA tag polyclonal antibody (51064-2-AP), anti-Myc (16286-1-AP), anti-509 AR TI CL E IN P RE SS DYKDDDDK (66008-4-Ig) and histone H3 (17168-1-AP) antibodies were from 510 protein tech; Anti-Phospho-(Ser/Thr) (ab117253) and anti-P300 (ab275378) antibodies 511 were from Abcam; anti-Ubiquitin ( sc-8017) antibody was from santa cruz; anti-Acetyl 512 lysine antibody (PTM-105RM) was from PTM BIO; DCAF15 antibody was from 513 Sigma; 3-methyladenine (3MA, HY-19312), Rapamycin (Rapa, HY-10219), MG-132 514 (HY-13259), Chloroquine (CQ, HY-17589A ), Pim1/AKK1-IN-1 (Pim1, HY-10371), 515 Crocetin monomethyl ester (HY-N6904), cycloheximide (HY-12320) and DMSO (HY-516 Y0320) were from MCE; Anti-Myc affinity gel (P2285), Protein A+G agarose (P2055), 517 GST-tag purification resin (P2251) and NP-40(P0013F) were from Beyotime; Anti-518 DYKDDDDK G1 affinity resin (L00432) was from Gen Script, Lipomaster 293 519 transfection reagent (JC3001) and ChamQ Blue Universal SYBR qPCR Master Mix 520 (Q312) were from Vazyme; LipofectamineTM 3000 (L3000008) was from Thermo 521 Scientific; SIRT1 Fusion Protein (Ag17677) and STK11 Fusion Protein (Ag1048) were 522 from protein tech. .. 523 Plasmid constructs and transfection 524 The plasmids including Flag-tagged DCAF15-K562R, DCAF15-K560R, 525 DCAF15-K581R, DCAF15-2KR, DCAF15-2KQ acquired from BGI (Beijing, China).

    Article Title: Dcaf15-mediated EphA2 degradation triggers disruption of the blood-brain barrier during Streptococcus suis meningitis
    Article Snippet: .. The following antibodies were used in this study: anti-EphA2 (6997), anti-LC3B (3868 T), anti-AKT (9272S), anti-Phospho-AKT (Ser473) (4060S), anti-Phospho-p44/42 MAPK (ERK1/2) (Thr202/Tyr204) (4370 T), anti-p44/42MAPK (ERK1/2) (4695 T), anti-AMPKα (5831), Anti-Phospho-AMPKα (Thr172) (2535) and Phospho-LKB1 (Ser334) (3055) antibodies were from Cell Signaling Technology; anti-DYKDDDDK tag (20543-1-AP), anti-P62/SQSTM1 (18420-1-AP), anti-STK11/LKB1 (10746-1-AP), anti-ZO-1 (21773-1-AP), anti-SIRT1 (60303-1-Ig), anti-HA tag polyclonal antibody (51064-2-AP), anti-Myc (16286-1-AP), anti-DYKDDDDK (66008-4-Ig) and histone H3 (17168-1-AP) antibodies were from protein tech; Anti-Phospho-(Ser/Thr) (ab117253) and anti-P300 (ab275378) antibodies were from Abcam; anti-Ubiquitin (sc-8017) antibody was from santa cruz; anti-Acetyl lysine antibody (PTM-105RM) was from PTM BIO; DCAF15 antibody was from Sigma; 3-methyladenine (3MA, HY-19312), Rapamycin (Rapa, HY-10219), MG-132 (HY-13259), Chloroquine (CQ, HY-17589A), Pim1/AKK1-IN-1 (Pim1, HY-10371), Crocetin monomethyl ester (HY-N6904), cycloheximide (HY-12320) and DMSO (HY-Y0320) were from MCE; Anti-Myc affinity gel (P2285), Protein A + G agarose (P2055), GST-tag purification resin (P2251) and NP-40(P0013F) were from Beyotime; Anti-DYKDDDDK G1 affinity resin (L00432) was from Gen Script, Lipomaster 293 transfection reagent (JC3001) and ChamQ Blue Universal SYBR qPCR Master Mix (Q312) were from Vazyme; LipofectamineTM 3000 (L3000008) was from Thermo Scientific; SIRT1 Fusion Protein (Ag17677) and STK11 Fusion Protein (Ag1048) were from protein tech. .. The plasmids, including Flag-tagged DCAF15-K562R, DCAF15-K560R, DCAF15-K581R, DCAF15-2KR, DCAF15-2KQ acquired from BGI (Beijing, China).



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