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aphidicolin  (MedChemExpress)


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    Structured Review

    MedChemExpress aphidicolin
    Aphidicolin, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hy-n6733/Aphidicolin/pmc12894886-36-0-5
    Average 95 stars, based on 30 article reviews
    aphidicolin - by Bioz Stars, 2026-10
    95/100 stars

    Images

    Related Articles

    Transferring:

    Article Title: Targeting SH3GL1 for prognosis and immune response in breast cancer
    Article Snippet: Cells were seeded in a 6-well plate coated with extracellular matrix (ECM) substrate (50 μg/mL poly- l -lysine, C0313-5 mg, Beyotime, Shanghai, China) at a ratio of 1 × 10 6 cells per well. .. A straight line was scratched using a pipette tip, and the remaining cells were further cultured in fresh medium supplemented with aphidicolin C (inhibitor of DNA replication, 10 μg/mL, HY-13316, MedChemExpress, Princeton, NJ, USA) for 12 h. After incubation, the cells were fixed with 3.7% paraformaldehyde (PFA) for 15 min and images were taken at 0 and 12 h. The area devoid of cells in the images was manually tracked using ImageJ software, and the wound closure percentage was calculated by the following formula: Wound closure (%) = (At = 0 h − At = 12 h)/At = 0 h × 100. ..

    Cell Culture:

    Article Title: Targeting SH3GL1 for prognosis and immune response in breast cancer
    Article Snippet: Cells were seeded in a 6-well plate coated with extracellular matrix (ECM) substrate (50 μg/mL poly- l -lysine, C0313-5 mg, Beyotime, Shanghai, China) at a ratio of 1 × 10 6 cells per well. .. A straight line was scratched using a pipette tip, and the remaining cells were further cultured in fresh medium supplemented with aphidicolin C (inhibitor of DNA replication, 10 μg/mL, HY-13316, MedChemExpress, Princeton, NJ, USA) for 12 h. After incubation, the cells were fixed with 3.7% paraformaldehyde (PFA) for 15 min and images were taken at 0 and 12 h. The area devoid of cells in the images was manually tracked using ImageJ software, and the wound closure percentage was calculated by the following formula: Wound closure (%) = (At = 0 h − At = 12 h)/At = 0 h × 100. ..

    Incubation:

    Article Title: Targeting SH3GL1 for prognosis and immune response in breast cancer
    Article Snippet: Cells were seeded in a 6-well plate coated with extracellular matrix (ECM) substrate (50 μg/mL poly- l -lysine, C0313-5 mg, Beyotime, Shanghai, China) at a ratio of 1 × 10 6 cells per well. .. A straight line was scratched using a pipette tip, and the remaining cells were further cultured in fresh medium supplemented with aphidicolin C (inhibitor of DNA replication, 10 μg/mL, HY-13316, MedChemExpress, Princeton, NJ, USA) for 12 h. After incubation, the cells were fixed with 3.7% paraformaldehyde (PFA) for 15 min and images were taken at 0 and 12 h. The area devoid of cells in the images was manually tracked using ImageJ software, and the wound closure percentage was calculated by the following formula: Wound closure (%) = (At = 0 h − At = 12 h)/At = 0 h × 100. ..

    Article Title: RNA polymerase II degradation triggered by DNA repair occurs in trans and independently of how the lesion is recognized
    Article Snippet: .. When indicated, cells were incubated with the following drugs, which were kept in culture medium until harvesting: IlludinS (MedChemExpress #HY-125098); MG132 (Selleckchem #S2619); Spironolactone (Selleckchem #S4054); α-amanitin (Sigma #A2263); Aphidicolin (MedChemExpress #HY-N6733); ATR inhibitor VE822 (Selleckchem # S7102); ATM inhibitor KU60019 (Selleckchem #S1570); DNA-PK inhibitor NU7441 (Selleckchem #S2638); GSK3 inhibitor CHIR-99021 (Sigma); 5,6-dichlorobenzimidazole 1-β-D-ribofuranoside (DRB) (Sigma #D1916); CDK7 inhibitor THZ1 (Selleckchem #S7449); CDK9 inhibitor BAY1251152 (Selleckchem #S8730); and MLN4924 (Selleckchem #S7109). .. The oligonucleotide duplex (AGCTTGCCGCCACCATGACTAGTTATCCTTACGATGTGCCAGACTACGCTAGCG; GATCCGCTAGCGTAGTCTGGCACATCGTAAGGATAACTAGTCATGGTGGCGGCA), containing the Kozak sequence and the HA epitope, was inserted into HindIII/BamHI-treated pcDNA5/FRT/TO vector (Thermo Fisher Scientific).

    Knock-Out:

    Article Title: RNA m 1 A methyltransferase TRMT61A promotes colorectal tumorigenesis by enhancing ONECUT2 mRNA stability and is a potential therapeutic target
    Article Snippet: .. To induce Trmt61a knockout, 6‐8 weeks old Apc Min/+ Trmt61a cko mice or their littermate controls were intraperitoneally administered tamoxifen (#HY‐13757A, MedChemExpress) at a dosage of 100 mg/kg. ..

    other:

    Article Title: LAP2α drives breast tumorigenesis by mitigating replication stress.
    Article Snippet: Rucaparib (AZD2281) and cisplatin (S1166) were purchased from Selleck.



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