quercetagitrin (MedChemExpress)
93
Structured Review
MedChemExpress
quercetagitrin

Quercetagitrin, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hy-n4150/Quercetagitrin/pmc10180163-128-10-12
Average 93 stars, based on 2 article reviews

Quercetagitrin, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hy-n4150/Quercetagitrin/pmc10180163-128-10-12
Average 93 stars, based on 2 article reviews
quercetagitrin - by Bioz Stars,
2026-10
93/100 stars
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1) Product Images from "Quercetagitrin Inhibits Tau Accumulation and Reverses Neuroinflammation and Cognitive Deficits in P301S-Tau Transgenic Mice"
Article Title: Quercetagitrin Inhibits Tau Accumulation and Reverses Neuroinflammation and Cognitive Deficits in P301S-Tau Transgenic Mice
Journal: Molecules
doi: 10.3390/molecules28093964
Figure Legend Snippet: Quercetagitrin inhibits tau aggregation in vitro. ( A ) Structure of quercetagitrin. ( B ) Heparin-induced fibrillization of tau-K18 protein monitored by ThT fluorescence in the presence of different concentrations of quercetagitrin (0, 3.12, 6.25,12.5, 25.0, 50.0, and 100 μM). ( C ) Representative TEM images of tau-K18 aggregation products with different concentration of quercetagitrin or control vehicle (scale bar = 0.2 μm).
Techniques Used: In Vitro, Fluorescence, Concentration Assay, Control
Figure Legend Snippet: Quercetagitrin decreases tau phosphorylation in HEK293 cells and N2a cells. ( A , B ) The viability of HEK293 cells ( A ) and N2a cells ( B ) was determined by CCK-8 assay in the presence of gradient concentration of quercetagitrin. N = 4 for each group, unpaired Student’s t test. ( C , D ) HEK293 cells were transfected with human 1N4R P301S-tau plasmids for 24 h and then incubated with quercetagitrin for another 24 h. ( C ) Representative western blots of AT8 (Ser202/Thr205), AT100 (Thr212/Ser214), AT180 (Thr231), AT270 (Thr181), and total tau (tau13). ( D ) Quantification analysis of phosphorylated tau and total tau levels normalized to GAPDH. N = 3 for each group, one-way ANOVA, Dunnett’s post hoc analysis. ( E , F ) N2a cells were transfected with human 1N4R P301S-tau plasmids for 24 h and then incubated with quercetagitrin for another 24 h. ( E ) Representative western blots of AT8 (Ser202/Thr205), AT100 (Thr212/Ser214), AT180 (Thr231), AT270 (Thr181), and total tau (tau13) are shown. ( F ) Quantification analysis of phosphorylated tau and total tau levels normalized to β-actin. N = 3 for each group, one-way ANOVA, Dunnett’s post hoc analysis. Data were expressed as mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001.
Techniques Used: Phospho-proteomics, CCK-8 Assay, Concentration Assay, Transfection, Incubation, Western Blot
Figure Legend Snippet: Quercetagitrin reduces tau phosphorylation in P301S-tau transgenic mice. After feeding with quercetagitrin for three months, tau pathology in nine-month-old male P301S-tau transgenic mice was analyzed. ( A , B ) Representative western blots of AT8 (Ser202/Thr205), AT100 (Thr212/Ser214), AT180 (Thr231), AT270 (Thr181), ace-tau(K174), and total tau (tau13) in the hippocampus ( A ) and cortex ( B ). ( C , D ) Quantification analysis of phosphor-tau, ace-tau and total tau levels in hippocampus normalized to GAPDH ( C ) and cortex normalized to β-actin ( D ). N = 4 for each group, unpaired Student’s t test. ( E ) Representative immunohistochemical images of anti-AT8 antibody staining in the hippocampus (upper) and cortex (lower) of P301S-tau transgenic mice (scale bar: 100 μm). ( F ) Quantification analysis of normalized AT8 positive area in the hippocampus and cortex. N = 4 for each group, unpaired Student’s t test. ( G – L ) Western blot analysis of PP2A-C, p-GSK3β(Y216), total GSK3β, and β-actin in the hippocampus ( G ) and cortex ( H ) of P301S-tau transgenic mice treated with quercetagitrin or vehicle. ( I – L ) Quantification analysis of PP2A-C normalized to β-actin and p-GSK3β(Y216) normalized to total GSK3β. N = 4 for each group, one-way ANOVA, Dunnett’s post hoc analysis. Data were expressed as mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001.
Techniques Used: Phospho-proteomics, Transgenic Assay, Western Blot, Immunohistochemical staining, Staining
Figure Legend Snippet: Quercetagitrin diminishes tau oligomerization and tau fibrillization in P301S-tau transgenic mice. ( A ) The oligomeric tau (~130 KDa, 250~460 KDa) in the hippocampus and cortex of P301S-tau transgenic mice treated with quercetagitrin or vehicle was measured by means of western blotting using anti-T22 antibody (which specifically reacts with oligomeric tau). ( B , C ) Quantification analysis of oligomeric tau (1×, 2×, 4× and 6×) in the hippocampus ( B ) and cortex ( C ). N = 4 for each group, unpaired Student’s t test. ( D ) Representative Thio-S staining images in the brain of control and quercetagitrin-treated P301S-tau transgenic mice (microscope: ZEISS LMS 880, object lens: 40×, scale bar: 20 μm). ( E , F ) Thio-S intensity analysis in the hippocampus ( E ) and cortex ( F ). N = 4 for each group, unpaired Student’s t test. Data were expressed as mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001.
Techniques Used: Transgenic Assay, Western Blot, Staining, Control, Microscopy
Figure Legend Snippet: Quercetagitrin treatment reverses gliosis and neuronal loss in P301S-tau transgenic mice. ( A ) Representative immunofluorescence images of astrocyte marker (GFAP), microglia marker (Iba1), and neuron marker (NeuN) in the hippocampus of WT mice and control or quercetagitrin-treated P301S-tau transgenic mice (microscope: ZEISS LMS 880, object lens: 10×, scale bar: 200 μm for the whole hippocampal images and 20 μm for the enlarged images). ( B , C ) Quantification analysis of the GFAP fluorescence intensities ( B ) and Iba1 fluorescence intensities ( C ) in the hippocampus of P301S-tau transgenic mice. N = 4 for each group, one-way ANOVA, Dunnett’s post hoc analysis. ( D ) Quantification analysis of neuron numbers in the hippocampal CA1, CA3, and DG subset, respectively. N = 4 for each group, one-way ANOVA, Dunnett’s post hoc analysis. Data were expressed as mean ± SEM, ** p < 0.01, *** p < 0.001.
Techniques Used: Transgenic Assay, Immunofluorescence, Marker, Control, Microscopy, Fluorescence
Figure Legend Snippet: Quercetagitrin treatment attenuates synaptic impairments and cognitive deficits in P301S-tau transgenic mice. ( A ) Representative immunofluorescence images showing expression levels of synaptophysin and NeuN in the hippocampal CA3 subset of WT and control or quercetagitrin-treated P301S-tau transgenic mice (microscope: ZEISS LMS 880, object lens: 20×, scale bar: 50 μm). Quantification analysis shows the relative synaptophysin fluorescence intensity in hippocampal CA3 subset. N = 4 for each group, one-way ANOVA, Dunnett’s post hoc analysis. ( B ) Quercetagitrin treatment restores spatial working memory in the Y-maze test. Quantification of the alternation of WT and control or quercetagitrin-treated P301S-tau transgenic mice in the Y-maze test. N = 7–10 for each group, one-way ANOVA, Dunnett’s post hoc analysis. ( C – H ) Quercetagitrin treatment attenuates cognitive deficits measured by Morris water maze (MWM). ( C ) The learning latency in the training phase. ( D ) The representative move trajectories of the mice. The quantification of latencies to reach the target platform region ( E ), target platform crossings ( F ), and time spent in the target quadrant ( G ) measured at day 6 after removing the hidden platform. ( H ) The swimming speed of the mice in the three groups. N = 7–10 for each group, one-way ANOVA, Dunnett’s post hoc analysis. Data were expressed as mean ± SEM, * p < 0.05, ** p < 0.01, *** p < 0.001.
Techniques Used: Transgenic Assay, Immunofluorescence, Expressing, Control, Microscopy, Fluorescence
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Article Title: Quercetagitrin Inhibits Tau Accumulation and Reverses Neuroinflammation and Cognitive Deficits in P301S-Tau Transgenic Mice. Article Snippet: this study, P301S-tau transgenic mice were randomly assigned to drug or vehicle groups. .. For the drug groups, the drinking water contained 10 µg/mL Article Title: Quercetagitrin Inhibits Tau Accumulation and Reverses Neuroinflammation and Cognitive Deficits in P301S-Tau Transgenic Mice. Article Snippet: aboratory animal center. In this study, P301S-tau transgenic mice were randomly assigned to drug or vehicle groups. For the drug groups, the drinking water contained 10 µg/mL quercetagitrin Article Title: Quercetagitrin Inhibits Tau Accumulation and Reverses Neuroinflammation and Cognitive Deficits in P301S-Tau Transgenic Mice Article Snippet: this study, P301S-tau transgenic mice were randomly assigned to drug or vehicle groups. .. For the drug groups, the Transfection:Article Title: Quercetagitrin Ameliorates Alzheimer-Like Pathologies Associated with TNFα/NFκB Pathway in APP/PS1 Mice Article Snippet: to food and water and were maintained under a 12 h light/dark cycle (lights on at 7:00 p.m., off at 7:00 a.m.) at a stable temperature (22 ± 2 °C). .. Article Title: Quercetagitrin Inhibits Tau Accumulation and Reverses Neuroinflammation and Cognitive Deficits in P301S-Tau Transgenic Mice. Article Snippet: this study, P301S-tau transgenic mice were randomly assigned to drug or vehicle groups. .. For the drug groups, the drinking water contained 10 µg/mL Article Title: Quercetagitrin Inhibits Tau Accumulation and Reverses Neuroinflammation and Cognitive Deficits in P301S-Tau Transgenic Mice. Article Snippet: aboratory animal center. In this study, P301S-tau transgenic mice were randomly assigned to drug or vehicle groups. 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For the drug groups, the drinking water contained 10 µg/mL Article Title: Quercetagitrin Inhibits Tau Accumulation and Reverses Neuroinflammation and Cognitive Deficits in P301S-Tau Transgenic Mice. Article Snippet: aboratory animal center. In this study, P301S-tau transgenic mice were randomly assigned to drug or vehicle groups. For the drug groups, the drinking water contained 10 µg/mL quercetagitrin Article Title: Quercetagitrin Inhibits Tau Accumulation and Reverses Neuroinflammation and Cognitive Deficits in P301S-Tau Transgenic Mice Article Snippet: this study, P301S-tau transgenic mice were randomly assigned to drug or vehicle groups. .. For the drug groups, the Marker:Article Title: Quercetagitrin Ameliorates Alzheimer-Like Pathologies Associated with TNFα/NFκB Pathway in APP/PS1 Mice Article Snippet: to food and water and were maintained under a 12 h light/dark cycle (lights on at 7:00 p.m., off at 7:00 a.m.) at a stable temperature (22 ± 2 °C). .. Article Title: Quercetagitrin Inhibits Tau Accumulation and Reverses Neuroinflammation and Cognitive Deficits in P301S-Tau Transgenic Mice. Article Snippet: this study, P301S-tau transgenic mice were randomly assigned to drug or vehicle groups. .. For the drug groups, the drinking water contained 10 µg/mL Article Title: Quercetagitrin Inhibits Tau Accumulation and Reverses Neuroinflammation and Cognitive Deficits in P301S-Tau Transgenic Mice. Article Snippet: aboratory animal center. In this study, P301S-tau transgenic mice were randomly assigned to drug or vehicle groups. For the drug groups, the drinking water contained 10 µg/mL quercetagitrin Article Title: Quercetagitrin Inhibits Tau Accumulation and Reverses Neuroinflammation and Cognitive Deficits in P301S-Tau Transgenic Mice Article Snippet: this study, P301S-tau transgenic mice were randomly assigned to drug or vehicle groups. .. For the drug groups, the Expressing:Article Title: Quercetagitrin Ameliorates Alzheimer-Like Pathologies Associated with TNFα/NFκB Pathway in APP/PS1 Mice Article Snippet: to food and water and were maintained under a 12 h light/dark cycle (lights on at 7:00 p.m., off at 7:00 a.m.) at a stable temperature (22 ± 2 °C). .. Article Title: Quercetagitrin Inhibits Tau Accumulation and Reverses Neuroinflammation and Cognitive Deficits in P301S-Tau Transgenic Mice. Article Snippet: this study, P301S-tau transgenic mice were randomly assigned to drug or vehicle groups. .. For the drug groups, the drinking water contained 10 µg/mL Article Title: Quercetagitrin Inhibits Tau Accumulation and Reverses Neuroinflammation and Cognitive Deficits in P301S-Tau Transgenic Mice. Article Snippet: aboratory animal center. In this study, P301S-tau transgenic mice were randomly assigned to drug or vehicle groups. For the drug groups, the drinking water contained 10 µg/mL quercetagitrin Article Title: Quercetagitrin Inhibits Tau Accumulation and Reverses Neuroinflammation and Cognitive Deficits in P301S-Tau Transgenic Mice Article Snippet: this study, P301S-tau transgenic mice were randomly assigned to drug or vehicle groups. .. For the drug groups, the |