rosamultin  (MedChemExpress)

Journal: Frontiers in Nutrition

Article Title: Construction of a Microfluidic Platform With Core-Shell CdSSe@ZnS Quantum Dot-Encoded Superparamagnetic Iron Oxide Microspheres for Screening and Locating Matrix Metalloproteinase-2 Inhibitors From Fruits of Rosa roxburghii

doi: 10.3389/fnut.2022.869528

Figure Lengend Snippet: Chromatographic and mass characteristics of MMP-2 binding ligands.

Article Snippet: Recombinant human matrix metalloproteinase-2 (expressed in HEK 293 cells), 1-(3-dimethyl-aminopropyl)-3-ethyl-carbodiimide (EDC), N -hydroxysuccinimide (NHS), and PEI (molecular weight: 750,000 Da) were purchased from Sigma Co. 2-(N-morpholino) ethanesulfonic acid (MES) was obtained from Shanghai Yien Chemical Technology Co. Oil-soluble core-shell CdSSe/ZnS QDs (5 mg·ml −1 ) with an emission wavelength at 594 nm and carboxyl terminated MSs (diameter: 5.53 μm and volume: 10 ml) were obtained from Hebei Langfei Biotechnology Co. Standard substances, namely, ellagic acid, loganic acid, corilagin, oreganol A, kajiichigoside F 1 , zebirioside O, niga-ichigoside F 1 , and quadranoside VIII, were purchased from Weikeqi Bio-technology Co. Peduncloside and valerenic acid were obtained from Shanghai Yuanye Bio-Technology Co. Potentillanoside A was supplied by Shanghai Tauto Biotech Co. Medicagenic acid was obtained from Chengdu Biopurify Phytochemicals Co. Rosamultin and batimastat were purchased from MedChemExpress Co. Purities of all the reference compounds were >98%.

Techniques: Binding Assay

Journal: Frontiers in Nutrition

Article Title: Construction of a Microfluidic Platform With Core-Shell CdSSe@ZnS Quantum Dot-Encoded Superparamagnetic Iron Oxide Microspheres for Screening and Locating Matrix Metalloproteinase-2 Inhibitors From Fruits of Rosa roxburghii

doi: 10.3389/fnut.2022.869528

Figure Lengend Snippet: Inhibitory effects of MMP-2 binding ligands.

Article Snippet: Recombinant human matrix metalloproteinase-2 (expressed in HEK 293 cells), 1-(3-dimethyl-aminopropyl)-3-ethyl-carbodiimide (EDC), N -hydroxysuccinimide (NHS), and PEI (molecular weight: 750,000 Da) were purchased from Sigma Co. 2-(N-morpholino) ethanesulfonic acid (MES) was obtained from Shanghai Yien Chemical Technology Co. Oil-soluble core-shell CdSSe/ZnS QDs (5 mg·ml −1 ) with an emission wavelength at 594 nm and carboxyl terminated MSs (diameter: 5.53 μm and volume: 10 ml) were obtained from Hebei Langfei Biotechnology Co. Standard substances, namely, ellagic acid, loganic acid, corilagin, oreganol A, kajiichigoside F 1 , zebirioside O, niga-ichigoside F 1 , and quadranoside VIII, were purchased from Weikeqi Bio-technology Co. Peduncloside and valerenic acid were obtained from Shanghai Yuanye Bio-Technology Co. Potentillanoside A was supplied by Shanghai Tauto Biotech Co. Medicagenic acid was obtained from Chengdu Biopurify Phytochemicals Co. Rosamultin and batimastat were purchased from MedChemExpress Co. Purities of all the reference compounds were >98%.

Techniques: Binding Assay, Control