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carboxyatractyloside  (MedChemExpress)


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    Structured Review

    MedChemExpress carboxyatractyloside
    Mitochondrial DHODH is normal in both structure and function. A. Simplified schematic diagram of N-terminal sequences of DHODH proteins. The N-terminus of DHDOH ( H. sapiens ) contains a mitochondrial signal (MS) and a transmembrane segment (TM). B. N-terminal sequence analysis of DHODH Mito (left) and DHODH Cyto (right). C. The DHODH proteins were imported into yeast mitochondria, followed by subjecting the mitochondria to proteinase K digestion in either isotonic or hypotonic buffer. Western blot analysis of DHODH was performed. ATG32, CHCHD4, and MTCO2 served as the controls for the outer mitochondrial membrane (OMM), intermembrane space (IMS), and inner mitochondrial membrane (IMM), respectively. D, E. Yeast mitochondria were treated with (D) oligomycin (10 μM) and <t>carboxyatractyloside</t> (20 μM), or (E) FCCP (5 μM); then, the mitochondria were incubated with the indicated DHODH proteins. Effects of inhibitors on the import of DHODH into yeast mitochondria were detected. F. DHODH activity of cytosolic and mitochondrial DHODH. Data are shown as mean ± SD. The statistical analyses were performed using the ANOVA. ns = not significant. FCCP, ionophore uncoupler trifluorocarbonylcyanide phenylhydrazone; SD, standard deviation; ANOVA, analysis of variance.
    Carboxyatractyloside, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hy-n2522/Carboxyatractyloside/pmc11152977-39-97-98
    Average 94 stars, based on 4 article reviews
    carboxyatractyloside - by Bioz Stars, 2026-10
    94/100 stars

    Images

    1) Product Images from "Redistribution of defective mitochondria-mediated dihydroorotate dehydrogenase imparts 5-fluorouracil resistance in colorectal cancer"

    Article Title: Redistribution of defective mitochondria-mediated dihydroorotate dehydrogenase imparts 5-fluorouracil resistance in colorectal cancer

    Journal: Redox Biology

    doi: 10.1016/j.redox.2024.103207

    Mitochondrial DHODH is normal in both structure and function. A. Simplified schematic diagram of N-terminal sequences of DHODH proteins. The N-terminus of DHDOH ( H. sapiens ) contains a mitochondrial signal (MS) and a transmembrane segment (TM). B. N-terminal sequence analysis of DHODH Mito (left) and DHODH Cyto (right). C. The DHODH proteins were imported into yeast mitochondria, followed by subjecting the mitochondria to proteinase K digestion in either isotonic or hypotonic buffer. Western blot analysis of DHODH was performed. ATG32, CHCHD4, and MTCO2 served as the controls for the outer mitochondrial membrane (OMM), intermembrane space (IMS), and inner mitochondrial membrane (IMM), respectively. D, E. Yeast mitochondria were treated with (D) oligomycin (10 μM) and carboxyatractyloside (20 μM), or (E) FCCP (5 μM); then, the mitochondria were incubated with the indicated DHODH proteins. Effects of inhibitors on the import of DHODH into yeast mitochondria were detected. F. DHODH activity of cytosolic and mitochondrial DHODH. Data are shown as mean ± SD. The statistical analyses were performed using the ANOVA. ns = not significant. FCCP, ionophore uncoupler trifluorocarbonylcyanide phenylhydrazone; SD, standard deviation; ANOVA, analysis of variance.
    Figure Legend Snippet: Mitochondrial DHODH is normal in both structure and function. A. Simplified schematic diagram of N-terminal sequences of DHODH proteins. The N-terminus of DHDOH ( H. sapiens ) contains a mitochondrial signal (MS) and a transmembrane segment (TM). B. N-terminal sequence analysis of DHODH Mito (left) and DHODH Cyto (right). C. The DHODH proteins were imported into yeast mitochondria, followed by subjecting the mitochondria to proteinase K digestion in either isotonic or hypotonic buffer. Western blot analysis of DHODH was performed. ATG32, CHCHD4, and MTCO2 served as the controls for the outer mitochondrial membrane (OMM), intermembrane space (IMS), and inner mitochondrial membrane (IMM), respectively. D, E. Yeast mitochondria were treated with (D) oligomycin (10 μM) and carboxyatractyloside (20 μM), or (E) FCCP (5 μM); then, the mitochondria were incubated with the indicated DHODH proteins. Effects of inhibitors on the import of DHODH into yeast mitochondria were detected. F. DHODH activity of cytosolic and mitochondrial DHODH. Data are shown as mean ± SD. The statistical analyses were performed using the ANOVA. ns = not significant. FCCP, ionophore uncoupler trifluorocarbonylcyanide phenylhydrazone; SD, standard deviation; ANOVA, analysis of variance.

    Techniques Used: Sequencing, Western Blot, Membrane, Incubation, Activity Assay, Standard Deviation

    Related Articles

    Purification:

    Article Title: Conformational change of adenine nucleotide translocase‐1 mediates cisplatin resistance induced by EBV‐LMP1
    Article Snippet: .. Reagents were purchased as follows: bongkrekic acid (19079, Cayman, USA), carboxyatractyloside (19079, MCE, China), cisplatin (HY‐17394, MCE, China), calcein‐AM (17783, Sigma‐Aldrich, USA), CoCl 2 (V900021, Sigma‐Aldrich, USA), calcimycin (A23187, HY‐N6687, MCE, China), Magnesium greenTM (M3733, Invitrogen, USA), STMRM, (T668, Invitrogen, USA), ATP Determination Kit (A22066, Invitrogen, USA), CCK‐8 (CK04, Dojindo, Japan), GST tag Purification Kit (P2262, Beyotime, China), MultiS Fast Mutagenesis Kit (C214, Vazym, China), 3,5‐difluoro‐L‐tyrosine (CAS 73246‐30‐7, Yiji Biotechnology, Shanghai, China). .. Antibodies used included ANT1 mAb (ab110322, Abcam, UK, 1:1,000), VDAC1 mAb (Ab34726, Abcam, UK, 1:1,000), HSP60 mAb (Sc‐1052, Santa Cruz Biotechnology, USA, 1:1,000), β‐actin mAb (A5441, Sigma, Germany, 1:10,000), cyto C mAb (556433, BD, USA, 1:1,000), TIM23 mAb (11123‐1‐AP, Proteintech, USA, 1:1,000), ANT2 mAb (14671S, CST, USA, 1:2,000), VDAC2 mAb (11663‐1‐AP, Proteintech, USA, 1:1,000), caspase‐9 mAb (9502S, CST, USA, 1:1,000), Flag mAb (F1804, Sigma‐Aldrich, USA, 1:1,000), and cleaved caspase‐3 mAb (9664S, CST, USA, 1:1,000).

    Mutagenesis:

    Article Title: Conformational change of adenine nucleotide translocase‐1 mediates cisplatin resistance induced by EBV‐LMP1
    Article Snippet: .. Reagents were purchased as follows: bongkrekic acid (19079, Cayman, USA), carboxyatractyloside (19079, MCE, China), cisplatin (HY‐17394, MCE, China), calcein‐AM (17783, Sigma‐Aldrich, USA), CoCl 2 (V900021, Sigma‐Aldrich, USA), calcimycin (A23187, HY‐N6687, MCE, China), Magnesium greenTM (M3733, Invitrogen, USA), STMRM, (T668, Invitrogen, USA), ATP Determination Kit (A22066, Invitrogen, USA), CCK‐8 (CK04, Dojindo, Japan), GST tag Purification Kit (P2262, Beyotime, China), MultiS Fast Mutagenesis Kit (C214, Vazym, China), 3,5‐difluoro‐L‐tyrosine (CAS 73246‐30‐7, Yiji Biotechnology, Shanghai, China). .. Antibodies used included ANT1 mAb (ab110322, Abcam, UK, 1:1,000), VDAC1 mAb (Ab34726, Abcam, UK, 1:1,000), HSP60 mAb (Sc‐1052, Santa Cruz Biotechnology, USA, 1:1,000), β‐actin mAb (A5441, Sigma, Germany, 1:10,000), cyto C mAb (556433, BD, USA, 1:1,000), TIM23 mAb (11123‐1‐AP, Proteintech, USA, 1:1,000), ANT2 mAb (14671S, CST, USA, 1:2,000), VDAC2 mAb (11663‐1‐AP, Proteintech, USA, 1:1,000), caspase‐9 mAb (9502S, CST, USA, 1:1,000), Flag mAb (F1804, Sigma‐Aldrich, USA, 1:1,000), and cleaved caspase‐3 mAb (9664S, CST, USA, 1:1,000).

    Acetylene Reduction Assay:

    Article Title: Redistribution of defective mitochondria-mediated dihydroorotate dehydrogenase imparts 5-fluorouracil resistance in colorectal cancer
    Article Snippet: .. In this study, cells and mice were treated with additional supplemental fatty acids (all from Sigma-Aldrich) including as palmitic acid (PA), oleic acid (OA), linoleic acid (LA), arachidonic acid (ARA), docosapentaenoic acid (DPA), alpha-linolenic acid (ALA), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA); fatty acid oxidation (FAO) inhibitor etomoxir; trifluorocarbonylcyanide phenylhydrazone (FCCP); chemotherapeutic agents including 5-FU (Abcam), irinotecan (MedChemExpress), and oxaliplatin (MedChemExpress); apoptosis inducer etoposide (Selleck); necroptosis inhibitor necrostatin-1 (MedChemExpress); ferroptosis inhibitors including ferrostatin-1 (MedChemExpress) and liproxstatin-1 (MedChemExpress); ferroptosis inducers including RSL3 (Selleck) and erastin (MedChemExpress); myristoylation inhibitor IMP-1088 (MedChemExpress); ATP production inhibitors including oligomycin (MedChemExpress) and carboxyatractyloside (MedChemExpress); and DHODH inhibitors including brequinar (Selleck) and teriflunomide (Selleck). .. HCT8, HCT15, HCT116, LoVo, SW480, HT-29, Caco-2, and T84 CRC cell lines were obtained from KeyGEN Biotech and Cyagen Biosciences.



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    carboxyatractyloside  (MedChemExpress)
    94
    MedChemExpress carboxyatractyloside
    Mitochondrial DHODH is normal in both structure and function. A. Simplified schematic diagram of N-terminal sequences of DHODH proteins. The N-terminus of DHDOH ( H. sapiens ) contains a mitochondrial signal (MS) and a transmembrane segment (TM). B. N-terminal sequence analysis of DHODH Mito (left) and DHODH Cyto (right). C. The DHODH proteins were imported into yeast mitochondria, followed by subjecting the mitochondria to proteinase K digestion in either isotonic or hypotonic buffer. Western blot analysis of DHODH was performed. ATG32, CHCHD4, and MTCO2 served as the controls for the outer mitochondrial membrane (OMM), intermembrane space (IMS), and inner mitochondrial membrane (IMM), respectively. D, E. Yeast mitochondria were treated with (D) oligomycin (10 μM) and <t>carboxyatractyloside</t> (20 μM), or (E) FCCP (5 μM); then, the mitochondria were incubated with the indicated DHODH proteins. Effects of inhibitors on the import of DHODH into yeast mitochondria were detected. F. DHODH activity of cytosolic and mitochondrial DHODH. Data are shown as mean ± SD. The statistical analyses were performed using the ANOVA. ns = not significant. FCCP, ionophore uncoupler trifluorocarbonylcyanide phenylhydrazone; SD, standard deviation; ANOVA, analysis of variance.
    Carboxyatractyloside, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hy-n2522/Carboxyatractyloside/pmc11152977-39-97-98
    Average 94 stars, based on 1 article reviews
    carboxyatractyloside - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    carboxyatractyloside cat  (MedChemExpress)
    94
    MedChemExpress carboxyatractyloside cat
    Mitochondrial DHODH is normal in both structure and function. A. Simplified schematic diagram of N-terminal sequences of DHODH proteins. The N-terminus of DHDOH ( H. sapiens ) contains a mitochondrial signal (MS) and a transmembrane segment (TM). B. N-terminal sequence analysis of DHODH Mito (left) and DHODH Cyto (right). C. The DHODH proteins were imported into yeast mitochondria, followed by subjecting the mitochondria to proteinase K digestion in either isotonic or hypotonic buffer. Western blot analysis of DHODH was performed. ATG32, CHCHD4, and MTCO2 served as the controls for the outer mitochondrial membrane (OMM), intermembrane space (IMS), and inner mitochondrial membrane (IMM), respectively. D, E. Yeast mitochondria were treated with (D) oligomycin (10 μM) and <t>carboxyatractyloside</t> (20 μM), or (E) FCCP (5 μM); then, the mitochondria were incubated with the indicated DHODH proteins. Effects of inhibitors on the import of DHODH into yeast mitochondria were detected. F. DHODH activity of cytosolic and mitochondrial DHODH. Data are shown as mean ± SD. The statistical analyses were performed using the ANOVA. ns = not significant. FCCP, ionophore uncoupler trifluorocarbonylcyanide phenylhydrazone; SD, standard deviation; ANOVA, analysis of variance.
    Carboxyatractyloside Cat, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hy-n2522/Carboxyatractyloside/bio_rxiv__2023__08__31__555665-109-18-23
    Average 94 stars, based on 1 article reviews
    carboxyatractyloside cat - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    Mitochondrial DHODH is normal in both structure and function. A. Simplified schematic diagram of N-terminal sequences of DHODH proteins. The N-terminus of DHDOH ( H. sapiens ) contains a mitochondrial signal (MS) and a transmembrane segment (TM). B. N-terminal sequence analysis of DHODH Mito (left) and DHODH Cyto (right). C. The DHODH proteins were imported into yeast mitochondria, followed by subjecting the mitochondria to proteinase K digestion in either isotonic or hypotonic buffer. Western blot analysis of DHODH was performed. ATG32, CHCHD4, and MTCO2 served as the controls for the outer mitochondrial membrane (OMM), intermembrane space (IMS), and inner mitochondrial membrane (IMM), respectively. D, E. Yeast mitochondria were treated with (D) oligomycin (10 μM) and carboxyatractyloside (20 μM), or (E) FCCP (5 μM); then, the mitochondria were incubated with the indicated DHODH proteins. Effects of inhibitors on the import of DHODH into yeast mitochondria were detected. F. DHODH activity of cytosolic and mitochondrial DHODH. Data are shown as mean ± SD. The statistical analyses were performed using the ANOVA. ns = not significant. FCCP, ionophore uncoupler trifluorocarbonylcyanide phenylhydrazone; SD, standard deviation; ANOVA, analysis of variance.

    Journal: Redox Biology

    Article Title: Redistribution of defective mitochondria-mediated dihydroorotate dehydrogenase imparts 5-fluorouracil resistance in colorectal cancer

    doi: 10.1016/j.redox.2024.103207

    Figure Lengend Snippet: Mitochondrial DHODH is normal in both structure and function. A. Simplified schematic diagram of N-terminal sequences of DHODH proteins. The N-terminus of DHDOH ( H. sapiens ) contains a mitochondrial signal (MS) and a transmembrane segment (TM). B. N-terminal sequence analysis of DHODH Mito (left) and DHODH Cyto (right). C. The DHODH proteins were imported into yeast mitochondria, followed by subjecting the mitochondria to proteinase K digestion in either isotonic or hypotonic buffer. Western blot analysis of DHODH was performed. ATG32, CHCHD4, and MTCO2 served as the controls for the outer mitochondrial membrane (OMM), intermembrane space (IMS), and inner mitochondrial membrane (IMM), respectively. D, E. Yeast mitochondria were treated with (D) oligomycin (10 μM) and carboxyatractyloside (20 μM), or (E) FCCP (5 μM); then, the mitochondria were incubated with the indicated DHODH proteins. Effects of inhibitors on the import of DHODH into yeast mitochondria were detected. F. DHODH activity of cytosolic and mitochondrial DHODH. Data are shown as mean ± SD. The statistical analyses were performed using the ANOVA. ns = not significant. FCCP, ionophore uncoupler trifluorocarbonylcyanide phenylhydrazone; SD, standard deviation; ANOVA, analysis of variance.

    Article Snippet: In this study, cells and mice were treated with additional supplemental fatty acids (all from Sigma-Aldrich) including as palmitic acid (PA), oleic acid (OA), linoleic acid (LA), arachidonic acid (ARA), docosapentaenoic acid (DPA), alpha-linolenic acid (ALA), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA); fatty acid oxidation (FAO) inhibitor etomoxir; trifluorocarbonylcyanide phenylhydrazone (FCCP); chemotherapeutic agents including 5-FU (Abcam), irinotecan (MedChemExpress), and oxaliplatin (MedChemExpress); apoptosis inducer etoposide (Selleck); necroptosis inhibitor necrostatin-1 (MedChemExpress); ferroptosis inhibitors including ferrostatin-1 (MedChemExpress) and liproxstatin-1 (MedChemExpress); ferroptosis inducers including RSL3 (Selleck) and erastin (MedChemExpress); myristoylation inhibitor IMP-1088 (MedChemExpress); ATP production inhibitors including oligomycin (MedChemExpress) and carboxyatractyloside (MedChemExpress); and DHODH inhibitors including brequinar (Selleck) and teriflunomide (Selleck).

    Techniques: Sequencing, Western Blot, Membrane, Incubation, Activity Assay, Standard Deviation