p36934 myristic acid medchemexpress  (MedChemExpress)

Journal: Molecular Biology Reports

Article Title: MARCKS N-terminal sequence-derived inhibitor peptides impair monocytic ROS production and migration via MARCKS-independent effects

doi: 10.1007/s11033-025-11027-8

Figure Lengend Snippet: MANS inhibits monocytic and neutrophilic ROS production. ( A ) Experimental design. Differentiated THP-1 (2 × 10 5 ) and PLB-985 cells (3 × 10 5 ) were preincubated with 100 µM MANS peptide or the scrambled control peptide RNS. ROS production was induced by 100 nM PMA, ops. E. coli , ops. S. aureus (at least 5 bacteria per cell), or 150 µg/ml ops. zymosan, and the induced ROS generation was measured for 3 h. Created with BioRender.com. ( B ) MANS inhibits ROS production in monocytic THP-1 cells. Kinetics of total ROS generation (representative experiments) of monocytic THP-1 cells in response to PMA, ops. E. coli and S. aureus bacteria, or ops. zymosan following pretreatment with MANS or RNS. ( C ) MANS inhibits ROS production in monocytic PLB-985 cells. Kinetics of total ROS generation (representative experiments) of MANS- or RNS-pretreated monocytic PLB-985 cells in response to PMA or ops. E. coli , S. aureus , and zymosan. ( D , E ) Cumulative total ROS production (mean ± SD) of the experiments performed as described in B ( n = 3–8) and C ( n = 3–4), respectively. ( F , G ) MANS inhibits ROS production in neutrophilic PLB-985 cells and primary human monocytes. Cumulative total ROS production of MANS- or RNS-pretreated neutrophilic (i.e., 3 days 1.25% DMSO-differentiated) PLB-985 cells ( F ) and primary human monocytes (2 × 10 5 , ROS measurement: 1 h; G ) in response to the indicated stimuli (mean ± SD, n = 3–5). Cumulative total ROS levels in the respective RNS-treated controls were set as 1 (dotted line). * p ≤ 0.05; ** p ≤ 0.01, *** p ≤ 0.005 in comparison to the RNS control; unpaired t test with Welch’s correction

Article Snippet: MANS (myristic acid–GAQFSKTAAKGEAAAERPGEAAVA), RNS (myristic acid–GTAPAAEGAGAEVKRASAEAKQAF) [ ], and BIO-11006 (acetyl-GAQFSKTAAK) [ ] were purchased from MedChemExpress (Sollentuna, Sweden).

Techniques: Control, Bacteria, Comparison

Journal: Molecular Biology Reports

Article Title: MARCKS N-terminal sequence-derived inhibitor peptides impair monocytic ROS production and migration via MARCKS-independent effects

doi: 10.1007/s11033-025-11027-8

Figure Lengend Snippet: MANS inhibits ROS production in both MARCKS WT and KO cells. ( A ) MANS inhibits ROS production in monocytic THP-1-derived WT and KO cells. Cumulative total ROS production in response to 100 nM PMA ( n = 7) and ops. E. coli (at least 5 bacteria per cell, n = 5) in 100 µM MANS-pretreated monocytic THP-1-derived MARCKS WT and KO clones (2 × 10 5 cells; mean ± SD). Kinetics of total ROS generation (representative experiments) and cumulative total ROS levels of ops. S. aureus - (at least 5 bacteria per cell, n = 6) and ops. zymosan-stimulated (150 µg/ml, n = 7) MARCKS WT and KO clones following MANS pretreatment (mean ± SD). ( B ) MANS inhibits monocytic ROS production of monocytic PLB-985-derived WT and KO cells. Cumulative total ROS production in response to PMA and ops. E. coli in MANS-pretreated monocytic PLB-985-derived MARCKS WT and KO clones (3 × 10 5 ; mean ± SD, n = 3, each data point represents the mean of WT1 and WT2 or KO1 and KO2, respectively). Kinetics of total ROS generation (representative experiments) and cumulative total ROS levels in MANS-pretreated and ops. S. aureus - and ops. zymosan-stimulated MARCKS WT and KO clones (mean ± SD, n = 3, each data point represents the mean of WT1 and WT2 or KO1 and KO2, respectively). Cumulative total ROS levels in the respective RNS-treated controls were set as 1 (dotted line). * p ≤ 0.05; ** p ≤ 0.01 in comparison to the RNS control; unpaired t test with Welch’s correction

Article Snippet: MANS (myristic acid–GAQFSKTAAKGEAAAERPGEAAVA), RNS (myristic acid–GTAPAAEGAGAEVKRASAEAKQAF) [ ], and BIO-11006 (acetyl-GAQFSKTAAK) [ ] were purchased from MedChemExpress (Sollentuna, Sweden).

Techniques: Derivative Assay, Bacteria, Clone Assay, Comparison, Control

Journal: Molecular Biology Reports

Article Title: MARCKS N-terminal sequence-derived inhibitor peptides impair monocytic ROS production and migration via MARCKS-independent effects

doi: 10.1007/s11033-025-11027-8

Figure Lengend Snippet: TNF preincubation enhances monocytic ROS production, but does not compensate for MANS treatment and/or MARCKS deficiency. ( A ) Experimental design. 2 × 10 5 monocytic THP-1 or THP-1-dervied MARCKS WT and KO cells were preincubated ± 80 ng/ml TNF for 48 h and 100 µM MANS or RNS for 30 min. ROS production was induced by ops. E. coli (at least 5 bacteria per cell) or 150 µg/ml ops. zymosan, and the induced ROS generation was measured. Created with BioRender.com. ( B ) TNF pretreatment enhances ROS production in monocytic THP-1 cells. Kinetics of total ROS production (representative experiments) of TNF-preincubated monocytic THP-1 cells in response to ops. E. coli and ops. zymosan. ( C ) Cumulative total ROS production of experiments performed as described in B (mean ± SD, n = 7). ( D ) TNF pretreatment enhances ROS production in monocytic THP-1-derived MARCKS WT and KO cells. Cumulative total ROS production in response to ops. E. coli and ops. zymosan in TNF-preincubated monocytic THP-1-derived MARCKS WT and KO clones (mean ± SD, n = 5). Cumulative total ROS levels in TNF-free THP-1 (C) or WT controls (D) were set as 1 (dotted line). ( E ) MANS inhibits monocytic ROS production in TNF-preincubated monocytic THP-1 cells. Cumulative total ROS production of TNF-preincubated and MANS-pretreated monocytic THP-1 cells in response to ops. E. coli and ops. zymosan (mean ± SD, n = 2–3). ( F ) MANS inhibits monocytic ROS production in TNF-preincubated monocytic THP-1-derived WT and KO cells. Cumulative total ROS production of TNF-preincubated and MANS-pretreated monocytic THP-1-derived WT and KO cells in response to ops. E. coli and ops. zymosan (mean ± SD, n = 3 each). Cumulative total ROS levels in RNS-treated cells were set as 1 (dotted line). * p ≤ 0.05; ** p ≤ 0.01, *** p ≤ 0.005; paired two-tailed ratio t test

Article Snippet: MANS (myristic acid–GAQFSKTAAKGEAAAERPGEAAVA), RNS (myristic acid–GTAPAAEGAGAEVKRASAEAKQAF) [ ], and BIO-11006 (acetyl-GAQFSKTAAK) [ ] were purchased from MedChemExpress (Sollentuna, Sweden).

Techniques: Bacteria, Derivative Assay, Clone Assay, Two Tailed Test

Journal: Molecular Biology Reports

Article Title: MARCKS N-terminal sequence-derived inhibitor peptides impair monocytic ROS production and migration via MARCKS-independent effects

doi: 10.1007/s11033-025-11027-8

Figure Lengend Snippet: MANS inhibits monocytic transmigration in MARCKS WT and KO cells. ( A ) Experimental design. Differentiated THP-1 (2 × 10 5 cells), PLB-985 (3 × 10 5 cells), and THP-1-/PLB-985-derived MARCKS WT and KO cells were preincubated with 100 µM MANS or RNS. Transmigration in response to 100 ng/ml LTB 4 , 50 ng/ml MCP-1, and/or 10% FCS was measured in transwell assays. Created with BioRender.com. ( B , C ) MANS and RNS inhibit chemoattractant-induced transmigration of monocytic THP-1 and PLB-985 cells. Relative number of transmigrated monocytic THP-1 ( B ) and PLB-985 ( C ) in response to the indicated chemoattractants following pretreatment with MANS or RNS (mean ± SD, n = 4). ( D , E ) MANS and RNS inhibit chemoattractant-induced transmigration of monocytic THP-1- and PLB-985-derived MARCKS WT and KO cells. Relative number of transmigrated monocytic THP-1- ( D ) and PLB-985-derived ( E ) WT and KO cells in response to the indicated chemoattractants following pretreatment with MANS or RNS (mean ± SD, n = 3; for PLB-985-derived WT and KO cells, each data point represents the mean of WT1 and WT2 or KO1 and KO2, respectively). Chemoattractant-induced transmigration in solvent-treated controls was set as 1 (dotted line). * p ≤ 0.05; ** p ≤ 0.01 in comparison to the solvent-treated control; unpaired t test with Welch’s correction

Article Snippet: MANS (myristic acid–GAQFSKTAAKGEAAAERPGEAAVA), RNS (myristic acid–GTAPAAEGAGAEVKRASAEAKQAF) [ ], and BIO-11006 (acetyl-GAQFSKTAAK) [ ] were purchased from MedChemExpress (Sollentuna, Sweden).

Techniques: Transmigration Assay, Derivative Assay, Solvent, Comparison, Control