dihydroxyflavone (MedChemExpress)
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MedChemExpress
dihydroxyflavone
Dihydroxyflavone, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hy-n1881/4'%2C5-Dihydroxyflavone/pmc13000954-44-0-6
Average 94 stars, based on 1 article reviews
Dihydroxyflavone, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hy-n1881/4'%2C5-Dihydroxyflavone/pmc13000954-44-0-6
Average 94 stars, based on 1 article reviews
dihydroxyflavone - by Bioz Stars,
2026-10
94/100 stars
Images
1) Product Images from "Scutellarein attenuates cancer cachexia-induced muscle atrophy via targeted inhibition of the JAK/STAT pathway"
Article Title: Scutellarein attenuates cancer cachexia-induced muscle atrophy via targeted inhibition of the JAK/STAT pathway
Journal: Journal of Advanced Research
doi: 10.1016/j.jare.2025.07.001
Figure Legend Snippet: Scutellarein protects against IFN-γ/TNF-α-induced myotube atrophy in C2C12 cells. Myotubes were treated with indicated concentrations of scutellarein and inflammatory cytokines, IFN-γ (20 ng/mL) and TNF-α (20 ng/mL). (A-C) Expression levels of phosphorylated and total JAK2 (A), STAT3, STAT5 (B), JNK, and p38 (C) were analyzed by immunoblotting. (D) Representative immunofluorescence images of MHC (green), Hoechst (blue), and merged images are shown, and (E) fusion index was quantified using ImageJ software. Scale bar: 200 µm. (F) Protein expression levels of MHC analyzed by immunoblotting. (G) mRNA expression of MyoD and myogenin was evaluated by qRT-PCR. (H) Protein synthesis following 24-hour treatment of scutellarein and IFN-γ/TNF-α was assessed using the SUnSET assay, with puromycin incorporation visualized by immunoblotting. GAPDH was used as a loading control. The data are presented as mean ± SD (n = 4–5). Statistical significance was determined by one-way ANOVA followed by Dunnett's multiple comparisons test. ## p < 0.01 vs. control group; * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. IFN-γ/TNF-α group. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Techniques Used: Expressing, Western Blot, Immunofluorescence, Software, Quantitative RT-PCR, Control
Figure Legend Snippet: Scutellarein attenuates CT26-CM-induced myotube atrophy in C2C12 cells. Myotubes were treated with indicated concentrations of scutellarein and CT26-CM. (A) Representative immunofluorescence images of MHC (green), DAPI (blue), and merged images are shown, and (B) fusion index was quantified using ImageJ software. Scale bar: 50 µm. Protein expression levels of (C) MHC, (D) MAFbx and MuRF1, (E) puromycin, and (F) phosphorylated and total JAK2 and STAT3 were analyzed by immunoblotting. GAPDH was used as a loading control. The data are presented as mean ± SD (n = 4–5). Statistical significance was determined by one-way ANOVA followed by Dunnett's multiple comparisons test. ### p < 0.001 vs. control group; ** p < 0.01, and *** p < 0.001 vs. CCM group. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Techniques Used: Immunofluorescence, Software, Expressing, Western Blot, Control
Figure Legend Snippet: Hydroxyl group positioning in flavones modulates JAK2 inhibition and STAT3 signaling. (A) Chemical structures of scutellarein and eight structurally related flavones. The structures of scutellarein and eight flavones, each containing two or three hydroxyl groups located at carbons 4′, 5, 6, and 7, are presented. (B) Inhibitory effects of scutellarein and eight flavones on JAK2 activity. The JAK2 kinase assay results for scutellarein and eight structurally related flavones are shown. Each flavone was tested at concentrations of 1 µM and 3.3 µM to evaluate their inhibitory effects on JAK2. (C-E) Myotubes were treated with flavones (10 µM) and inflammatory cytokines, IFN-γ (20 ng/mL) and TNF-α (20 ng/mL). The mRNA expression of (C) MyoD, myogenin, and (D) PGC-1α was evaluated by qRT-PCR. (E) Expression levels of phosphorylated and total STAT3 were analyzed by immunoblotting. GAPDH was used as a loading control. (F) Predicted binding pose of scutellarein with JAK2 (PDB ID: 4F09). The compound is shown in magenta, while the protein is in silver cartoon and key residues in green sticks. A water molecule taken from the crystal structure was shown as a small red sphere. Statistical significance was determined by one-way ANOVA followed by Dunnett's multiple comparisons test. ### p < 0.001 vs. control group; * p < 0.05 and *** p < 0.001 vs. IFN-γ/TNF-α group. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Techniques Used: Inhibition, Activity Assay, Kinase Assay, Expressing, Quantitative RT-PCR, Western Blot, Control, Binding Assay
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