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MedChemExpress levistolide a

The cytotoxicity of <t>Levistolide</t> <t>A</t> on Vero and LLC-PK1 cells. Cells were treated with LA (20, 40, 60, 80, or 100 µM); DMSO served as the treatment control. The cell viability value was measured by CCK8 assay 48 h later. The value of each group was normalized by the average value (set at 100%) of the mock-treated group. ( A ) The cell viability of the Vero cells exposed to LA at designed concentrations. ( B ) The cell viability of LLC-PK1 cells exposed to LA at designed concentrations. Data represent the mean ± standard deviation (SD) of three independent experiments, and error bars represent standard deviation.

Levistolide A, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hy-n1472/Levistolide+A/pmc08878026-64-0-7
Average 94 stars, based on 4 article reviews

levistolide a - by Bioz Stars, 2026-10

94/100 stars

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1) Product Images from "Levistolide A Inhibits PEDV Replication via Inducing ROS Generation"

Article Title: Levistolide A Inhibits PEDV Replication via Inducing ROS Generation

Journal: Viruses

doi: 10.3390/v14020258

The cytotoxicity of Levistolide A on Vero and LLC-PK1 cells. Cells were treated with LA (20, 40, 60, 80, or 100 µM); DMSO served as the treatment control. The cell viability value was measured by CCK8 assay 48 h later. The value of each group was normalized by the average value (set at 100%) of the mock-treated group. ( A ) The cell viability of the Vero cells exposed to LA at designed concentrations. ( B ) The cell viability of LLC-PK1 cells exposed to LA at designed concentrations. Data represent the mean ± standard deviation (SD) of three independent experiments, and error bars represent standard deviation.

Figure Legend Snippet: The cytotoxicity of Levistolide A on Vero and LLC-PK1 cells. Cells were treated with LA (20, 40, 60, 80, or 100 µM); DMSO served as the treatment control. The cell viability value was measured by CCK8 assay 48 h later. The value of each group was normalized by the average value (set at 100%) of the mock-treated group. ( A ) The cell viability of the Vero cells exposed to LA at designed concentrations. ( B ) The cell viability of LLC-PK1 cells exposed to LA at designed concentrations. Data represent the mean ± standard deviation (SD) of three independent experiments, and error bars represent standard deviation.

Techniques Used: Control, CCK-8 Assay, Standard Deviation

Levistolide A exhibits antiviral activity against PEDV strain DR13 and YN15 in Vero and LLC-PK1 cells. Vero cells were treated with LA at indicated concentrations and infected with PEDV at an MOI of 0.1 at the same time. DMSO served as the treatment control. Samples were collected at 36 hpi for IFA or TCID 50 assay analysis. ( A ) Immunofluorescence of PEDV-S protein (green) detected in PEDV strain DR13 infected Vero cells. ( B ) The viral titer determined in PEDV strain DR13 infected Vero cells. ( C ) Viral titer determined in PEDV strain YN15 infected Vero cells. ( D , E ) Vero cells were treated with LA (80 µM) or DMSO, and exposed to PEDV at 0.25 MOI or 0.5 MOI. Viral titers were assessed at 36 hpi. ( F , G ) Vero cells were treated with LA (80 µM) or DMSO, and exposed to PEDV (0.1 MOI). Viral titers were assessed at designed hpi. ( H ) Viral titer determined in the PEDV strain DR13 infected LLC-PK1 cells. ( I ) The viral titer determined in PEDV strain YN15 infected LLC-PK1 cells. Viral titer level was represented by the value of −lg TCID 50 /0.1 mL. Data represent the mean ± standard deviation (SD) of three independent experiments, and error bars represent standard deviation. Asterisks indicate significant differences between mock-treated group and LA or DMSO treated group: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p ≤ 0.0001; ns, not significant.

Figure Legend Snippet: Levistolide A exhibits antiviral activity against PEDV strain DR13 and YN15 in Vero and LLC-PK1 cells. Vero cells were treated with LA at indicated concentrations and infected with PEDV at an MOI of 0.1 at the same time. DMSO served as the treatment control. Samples were collected at 36 hpi for IFA or TCID 50 assay analysis. ( A ) Immunofluorescence of PEDV-S protein (green) detected in PEDV strain DR13 infected Vero cells. ( B ) The viral titer determined in PEDV strain DR13 infected Vero cells. ( C ) Viral titer determined in PEDV strain YN15 infected Vero cells. ( D , E ) Vero cells were treated with LA (80 µM) or DMSO, and exposed to PEDV at 0.25 MOI or 0.5 MOI. Viral titers were assessed at 36 hpi. ( F , G ) Vero cells were treated with LA (80 µM) or DMSO, and exposed to PEDV (0.1 MOI). Viral titers were assessed at designed hpi. ( H ) Viral titer determined in the PEDV strain DR13 infected LLC-PK1 cells. ( I ) The viral titer determined in PEDV strain YN15 infected LLC-PK1 cells. Viral titer level was represented by the value of −lg TCID 50 /0.1 mL. Data represent the mean ± standard deviation (SD) of three independent experiments, and error bars represent standard deviation. Asterisks indicate significant differences between mock-treated group and LA or DMSO treated group: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p ≤ 0.0001; ns, not significant.

Techniques Used: Activity Assay, Infection, Control, Immunofluorescence, Standard Deviation

Levistolide A disturbed viral RNA and protein production in PEDV-infected cells. Vero cells were treated with LA at indicated concentrations and infected with PEDV at an MOI of 0.1 at the same time. DMSO served as the treatment control. The samples were collected at 36 hpi for TaqMan-based real-time RT-PCR or Western blot analysis. ( A ) Viral RNA copies determined in the PEDV strain DR13 infected cells; ( B ) viral RNA copies determined in PEDV strain CV777 infected cells; ( C ) viral RNA copies determined in PEDV strain YN15 infected cells. ( D ) The amount of PEDV N protein in samples was detected through Western blot; β-actin was set as the loading control. Data represent the mean ± standard deviation (SD) of three independent experiments, and error bars represent standard deviation. Asterisks indicate significant differences between the mock-treated group and LA or DMSO treated group: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p ≤ 0.0001; ns, not significant.

Figure Legend Snippet: Levistolide A disturbed viral RNA and protein production in PEDV-infected cells. Vero cells were treated with LA at indicated concentrations and infected with PEDV at an MOI of 0.1 at the same time. DMSO served as the treatment control. The samples were collected at 36 hpi for TaqMan-based real-time RT-PCR or Western blot analysis. ( A ) Viral RNA copies determined in the PEDV strain DR13 infected cells; ( B ) viral RNA copies determined in PEDV strain CV777 infected cells; ( C ) viral RNA copies determined in PEDV strain YN15 infected cells. ( D ) The amount of PEDV N protein in samples was detected through Western blot; β-actin was set as the loading control. Data represent the mean ± standard deviation (SD) of three independent experiments, and error bars represent standard deviation. Asterisks indicate significant differences between the mock-treated group and LA or DMSO treated group: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p ≤ 0.0001; ns, not significant.

Techniques Used: Infection, Control, Quantitative RT-PCR, Western Blot, Standard Deviation

Levistolide A exhibits an inhibitory effect on the PEDV-invading Vero cells. ( A ) Vero cells were exposed to PEDV (0.1 MOI) and treated with 80 µM LA or DMSO during 0–2 hpi, then the cultured supernatant was replaced by PEDV culture medium containing LA or DMSO at 2, 4, 6, 8 hpi. The cultured supernatant in all the groups was replaced by PEDV culture medium after 10 hpi and samples were collected at 36 hpi. ( B ) The viral titer determined in the PEDV strain DR13 infected Vero cells. ( C ) The viral titer determined in the PEDV strain YN15 infected Vero cells. Data represent the mean ± standard deviation (SD) of three independent experiments, and error bars represent standard deviation. Asterisks indicate significant differences between virus control group and each LA treated group: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p ≤ 0.0001; ns, not significant.

Figure Legend Snippet: Levistolide A exhibits an inhibitory effect on the PEDV-invading Vero cells. ( A ) Vero cells were exposed to PEDV (0.1 MOI) and treated with 80 µM LA or DMSO during 0–2 hpi, then the cultured supernatant was replaced by PEDV culture medium containing LA or DMSO at 2, 4, 6, 8 hpi. The cultured supernatant in all the groups was replaced by PEDV culture medium after 10 hpi and samples were collected at 36 hpi. ( B ) The viral titer determined in the PEDV strain DR13 infected Vero cells. ( C ) The viral titer determined in the PEDV strain YN15 infected Vero cells. Data represent the mean ± standard deviation (SD) of three independent experiments, and error bars represent standard deviation. Asterisks indicate significant differences between virus control group and each LA treated group: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p ≤ 0.0001; ns, not significant.

Techniques Used: Cell Culture, Infection, Standard Deviation, Virus, Control

Antiviral activity of Levistolide A against PEDV is associated with inducing ROS production. ( A ) The level of ROS in Vero cells or LLC-PK1 cells after incubation with LA (80 µM) for 24 h. The value of each group was normalized by the average value (set at 1) of the corresponding DMSO treated group. ( B ) Viral titer level in LA/NAC, LA, NAC, or DMSO treated group after exposing to PEDV for 36 h. Viral titer level was represented by value of − lg TCID50/0.1 mL. ( C ) Immunofluorescence of PEDV-S protein (green) detected in LA/NAC, LA, NAC, or DMSO treated group after exposing to PEDV for 36 h. Data represent the mean ± standard deviation (SD) of three independent experiments, and error bars represent standard deviation. Asterisks indicate significant differences between virus control group and each LA treated group: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p ≤ 0.0001; ns, not significant.

Figure Legend Snippet: Antiviral activity of Levistolide A against PEDV is associated with inducing ROS production. ( A ) The level of ROS in Vero cells or LLC-PK1 cells after incubation with LA (80 µM) for 24 h. The value of each group was normalized by the average value (set at 1) of the corresponding DMSO treated group. ( B ) Viral titer level in LA/NAC, LA, NAC, or DMSO treated group after exposing to PEDV for 36 h. Viral titer level was represented by value of − lg TCID50/0.1 mL. ( C ) Immunofluorescence of PEDV-S protein (green) detected in LA/NAC, LA, NAC, or DMSO treated group after exposing to PEDV for 36 h. Data represent the mean ± standard deviation (SD) of three independent experiments, and error bars represent standard deviation. Asterisks indicate significant differences between virus control group and each LA treated group: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p ≤ 0.0001; ns, not significant.

Techniques Used: Activity Assay, Incubation, Immunofluorescence, Standard Deviation, Virus, Control

Levistolide A stimulated ER stress in Vero cells. ( A ) Tunicamycin (TM) potently inhibited PEDV replication. Vero cells exposed to PEDV strain YN15 (0.1 MOI) were incubated with 500 nM TM, samples were collected at 12, 24, and 36 hpi for TaqMan based real-time PCR; ( B ) mRNA level of ER stress-related genes increased in LA Vero cells. ( C ) The amount of GRP78 increased by treating with LA in either PEDV or mock infected Vero cells. PEDV strain YN15 (0.1 MOI) or mock infected Vero cells were incubated with LA or DMSO for 36 hpi. Western blot was performed to assess protein expression level and β-actin was set as loading control. Bands for GRP78 were marked by a black arrow. Data represent the mean ± standard deviation (SD) of three independent experiments, and error bars represent standard deviation. Asterisks indicate significant differences between virus control group and each LA treated group: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p ≤ 0.0001; ns, not significant.

Figure Legend Snippet: Levistolide A stimulated ER stress in Vero cells. ( A ) Tunicamycin (TM) potently inhibited PEDV replication. Vero cells exposed to PEDV strain YN15 (0.1 MOI) were incubated with 500 nM TM, samples were collected at 12, 24, and 36 hpi for TaqMan based real-time PCR; ( B ) mRNA level of ER stress-related genes increased in LA Vero cells. ( C ) The amount of GRP78 increased by treating with LA in either PEDV or mock infected Vero cells. PEDV strain YN15 (0.1 MOI) or mock infected Vero cells were incubated with LA or DMSO for 36 hpi. Western blot was performed to assess protein expression level and β-actin was set as loading control. Bands for GRP78 were marked by a black arrow. Data represent the mean ± standard deviation (SD) of three independent experiments, and error bars represent standard deviation. Asterisks indicate significant differences between virus control group and each LA treated group: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p ≤ 0.0001; ns, not significant.

Techniques Used: Incubation, Real-time Polymerase Chain Reaction, Infection, Western Blot, Expressing, Control, Standard Deviation, Virus

Enzyme-linked Immunosorbent Assay:

Article Title: Levistolide A Alleviates Myocardial Ischemia-Reperfusion Injury Partly by Improving Calcium Homeostasis via the ADORA2B/cAMP/PKA/PLB/SERCA2α Signaling Axis.
Article Snippet: .. The main reagents used in the experiment are as follows: Levistolide A (HY-N1472), ADORA2B receptor antagonist MRS 1754 (HY-14121), and thapsigargin (HY-13433) were purchased from MedChemExpress (Monmouth Junction, NJ, USA); Rat creatine kinase isoenzyme MB (CK-MB) ELISA kit (CB10461) and rat cardiac troponin I (cTnI) ELISA kit (CB11507) were provided by Shanghai Keaibo Biotechnology Co., Ltd. (Shanghai, China); Lactate dehydrogenase (LDH) activity assay kit (A020-2-1) and ultra-micro Ca2+-ATPase activity assay kit (A070-4-2) were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China); cAMP ELISA research kit (MM-926395O1) was purchased from Jiangsu Enzyme Immunoengineering Co., Ltd. (Yancheng, China); PKA activity assay kit (EIAPKA) was purchased from Thermo Fisher Scientific Inc. (Waltham, MA, USA); CCK8 cell proliferation and cytotoxicity assay kit (HYCCK8-500T) was provided by Wuhan Huiyucheng Biotechnology Co., Ltd. (Wuhan, China); Fluo-3 AM cell-permeable calcium fluorescent probe (40703ES50) was purchased from Yeasen Biotechnology (Shanghai) Co., Ltd. (Shanghai, China); DAPI nuclear staining reagent (C1002) was purchased from Beyotime Biotechnology (Shanghai) Co., Ltd. (Shanghai, China). .. The antibodies used in this study are as follows: adenosine A2B receptor (ADORA2B) (37KDa) antibody (bs-5900R) was purchased from Bioss Biotechnology Co., Ltd. (Beijing, China); phospholamban (PLB) (36KDa) antibody (A01395-1) was purchased from Boster Biological Engineering Co., Ltd. (Wuhan, China); phosphorylated phospholamban-Ser16 (p-PLB-Ser16) (36KDa) antibody (AP0907) was purchased from ABclonal Biotechnology Co., Ltd. (Wuhan, China); SERCA2α (140KDa) antibody (9580T) was purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA); and α-Tubulin (55 KDa) antibody (66031-1-Ig) was purchased from Proteintech Group, Inc. (Wuhan, China).

Article Title: Levistolide A Alleviates Myocardial Ischemia–Reperfusion Injury Partly by Improving Calcium Homeostasis via the ADORA2B/cAMP/PKA/PLB/SERCA2α Signaling Axis
Article Snippet: .. The main reagents used in the experiment are as follows: Levistolide A (HY-N1472), ADORA2B receptor antagonist MRS 1754 (HY-14121), and thapsigargin (HY-13433) were purchased from MedChemExpress (Monmouth Junction, NJ, USA); Rat creatine kinase isoenzyme MB (CK-MB) ELISA kit (CB10461) and rat cardiac troponin I (cTnI) ELISA kit (CB11507) were provided by Shanghai Keaibo Biotechnology Co., Ltd. (Shanghai, China); Lactate dehydrogenase (LDH) activity assay kit (A020-2-1) and ultra-micro Ca 2+ -ATPase activity assay kit (A070-4-2) were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China); cAMP ELISA research kit (MM-926395O1) was purchased from Jiangsu Enzyme Immunoengineering Co., Ltd. (Yancheng, China); PKA activity assay kit (EIAPKA) was purchased from Thermo Fisher Scientific Inc. (Waltham, MA, USA); CCK8 cell proliferation and cytotoxicity assay kit (HYCCK8-500T) was provided by Wuhan Huiyucheng Biotechnology Co., Ltd. (Wuhan, China); Fluo-3 AM cell-permeable calcium fluorescent probe (40703ES50) was purchased from Yeasen Biotechnology (Shanghai) Co., Ltd. (Shanghai, China); DAPI nuclear staining reagent (C1002) was purchased from Beyotime Biotechnology (Shanghai) Co., Ltd. (Shanghai, China). .. The antibodies used in this study are as follows: adenosine A2B receptor (ADORA2B) (37KDa) antibody (bs-5900R) was purchased from Bioss Biotechnology Co., Ltd. (Beijing, China); phospholamban (PLB) (36KDa) antibody (A01395-1) was purchased from Boster Biological Engineering Co., Ltd. (Wuhan, China); phosphorylated phospholamban-Ser16 (p-PLB-Ser16) (36KDa) antibody (AP0907) was purchased from ABclonal Biotechnology Co., Ltd. (Wuhan, China); SERCA2α (140KDa) antibody (9580T) was purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA); and α-Tubulin (55 KDa) antibody (66031-1-Ig) was purchased from Proteintech Group, Inc. (Wuhan, China).

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Article Title: Exploring the mechanism of Levistolide A in the treatment of lung cancer cells based on network analysis, molecular docking and experimental validation.
Article Snippet: Levistolide A (LA) is the main active ingredient in the Angelica sinensis (Oliv.). Diels, which has been proved to have therapeutic effects on a variety of tumors, but its role in treating lung cancer remains unreported.. In this study, we found that LA can inhibit lung cancer cell lines (A549 and H1299) proliferation and induced apoptosis via CCK-8, plate cloning, EdU labeling, flow cytometry and western blotting assay.

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