p bursaria cells (MedChemExpress)
Structured Review

P Bursaria Cells, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hy-n1446s2/Oleic+acid-13C18/bio_rxiv__2025__11__27__690892-222-0-12
Average 93 stars, based on 2 article reviews
Images
1) Product Images from "Host-derived lipid transfer and metabolic reprogramming in a ciliate–algal symbiosis"
Article Title: Host-derived lipid transfer and metabolic reprogramming in a ciliate–algal symbiosis
Journal: bioRxiv
doi: 10.1101/2025.11.27.690892
Figure Legend Snippet: (A) Pie and bar charts showing the proportion and number of each lipid class identified in isolated lipid droplets. (B) Heatmap of Pearson correlation coefficient matrix of lipidomic profiles showing distinct clustering of four biological replicates from symbiotic and aposymbiotic P. bursaria lipid droplets, G: symbiotic, W: aposymbiotic. (C) Fold-change comparison of total lipid abundance between symbiotic and aposymbiotic P. bursaria lipid droplets. (D) Distribution of lipid class abundance in symbiotic and aposymbiotic P. bursaria lipid droplets, DK2g: symbiotic, DK2w: aposymbiotic. (E) Differential abundance of individual lipid species between symbiotic and aposymbiotic P. bursaria lipid droplets.
Techniques Used: Isolation, Comparison
Figure Legend Snippet: Fold-change comparison of the abundance of each lipid class between symbiotic and aposymbiotic P. bursaria lipid droplets.
Techniques Used: Comparison
Figure Legend Snippet: (A) Transmission electron microscopy (TEM) images and quantitative analysis showing an increase in lipid droplet size in symbiotic Chlorella cells compared to free-living Chlorella cells. FL: free-living, SM: symbiotic, *** p-value < 0.001, Mann-Whitney U test, n=30 cells (B) Nanoscale secondary ion mass spectrometry (NanoSIMS) images showing incorporation of 13C isotope signals into symbiotic Chlorella cells after P. bursaria was fed with 13C-labeled oleic acid and quantitative analysis showing an increase in 13C/12C ratio in symbiotic Chlorella cells compared to free-living Chlorella cells. *** p-value < 0.001, Mann-Whitney U test, n=30 cells. (C) Elemental analysis–isotope ratio mass spectrometry (EA–IRMS) results showing a significantly higher 13C atom percentage in symbiotic Chlorella cells after P. bursaria was fed with 13C-labeled oleic acid. *** p-value < 0.001, Student’s t-tests, 4 biological replicates.
Techniques Used: Transmission Assay, Electron Microscopy, MANN-WHITNEY, Mass Spectrometry, Labeling
Figure Legend Snippet: Lipid droplet enlargement observed in Paramecium bursaria after feeding with oleic acid, demonstrating incorporation of exogenous fatty acid into host lipid droplets.
Techniques Used:
Related Articles
Isolation:Article Title: Host-derived lipid transfer and metabolic reprogramming in a ciliate–algal symbiosis Article Snippet: P. bursaria cells were fed with 0.1mM 13C-labeled oleic acid (13C18-oleic acid, Article Title: Host-derived lipid transfer and metabolic reprogramming in a ciliate–algal symbiosis Article Snippet: PrePrint: for MS1 and 0.05 Da for MS/MS, with a retention-time tolerance of 0.5 min for lipids. A score cutoff of 75 was applied. P. bursaria cells were fed with 0.1mM 13C-labeled oleic acid (13C18-oleic acid, Comparison:Article Title: Host-derived lipid transfer and metabolic reprogramming in a ciliate–algal symbiosis Article Snippet: P. bursaria cells were fed with 0.1mM 13C-labeled oleic acid (13C18-oleic acid, Article Title: Host-derived lipid transfer and metabolic reprogramming in a ciliate–algal symbiosis Article Snippet: PrePrint: for MS1 and 0.05 Da for MS/MS, with a retention-time tolerance of 0.5 min for lipids. A score cutoff of 75 was applied. P. bursaria cells were fed with 0.1mM 13C-labeled oleic acid (13C18-oleic acid, Transmission Assay:Article Title: Host-derived lipid transfer and metabolic reprogramming in a ciliate–algal symbiosis Article Snippet: P. bursaria cells were fed with 0.1mM 13C-labeled oleic acid (13C18-oleic acid, Article Title: Host-derived lipid transfer and metabolic reprogramming in a ciliate–algal symbiosis Article Snippet: PrePrint: for MS1 and 0.05 Da for MS/MS, with a retention-time tolerance of 0.5 min for lipids. A score cutoff of 75 was applied. P. bursaria cells were fed with 0.1mM 13C-labeled oleic acid (13C18-oleic acid, Electron Microscopy:Article Title: Host-derived lipid transfer and metabolic reprogramming in a ciliate–algal symbiosis Article Snippet: P. bursaria cells were fed with 0.1mM 13C-labeled oleic acid (13C18-oleic acid, Article Title: Host-derived lipid transfer and metabolic reprogramming in a ciliate–algal symbiosis Article Snippet: PrePrint: for MS1 and 0.05 Da for MS/MS, with a retention-time tolerance of 0.5 min for lipids. A score cutoff of 75 was applied. P. bursaria cells were fed with 0.1mM 13C-labeled oleic acid (13C18-oleic acid, MANN-WHITNEY:Article Title: Host-derived lipid transfer and metabolic reprogramming in a ciliate–algal symbiosis Article Snippet: P. bursaria cells were fed with 0.1mM 13C-labeled oleic acid (13C18-oleic acid, Article Title: Host-derived lipid transfer and metabolic reprogramming in a ciliate–algal symbiosis Article Snippet: PrePrint: for MS1 and 0.05 Da for MS/MS, with a retention-time tolerance of 0.5 min for lipids. A score cutoff of 75 was applied. P. bursaria cells were fed with 0.1mM 13C-labeled oleic acid (13C18-oleic acid, Mass Spectrometry:Article Title: Host-derived lipid transfer and metabolic reprogramming in a ciliate–algal symbiosis Article Snippet: P. bursaria cells were fed with 0.1mM 13C-labeled oleic acid (13C18-oleic acid, Article Title: Host-derived lipid transfer and metabolic reprogramming in a ciliate–algal symbiosis Article Snippet: PrePrint: for MS1 and 0.05 Da for MS/MS, with a retention-time tolerance of 0.5 min for lipids. A score cutoff of 75 was applied. P. bursaria cells were fed with 0.1mM 13C-labeled oleic acid (13C18-oleic acid, Labeling:Article Title: Host-derived lipid transfer and metabolic reprogramming in a ciliate–algal symbiosis Article Snippet: P. bursaria cells were fed with 0.1mM 13C-labeled oleic acid (13C18-oleic acid, Article Title: Host-derived lipid transfer and metabolic reprogramming in a ciliate–algal symbiosis Article Snippet: PrePrint: for MS1 and 0.05 Da for MS/MS, with a retention-time tolerance of 0.5 min for lipids. A score cutoff of 75 was applied. P. bursaria cells were fed with 0.1mM 13C-labeled oleic acid (13C18-oleic acid, Tandem Mass Spectroscopy:Article Title: Host-derived lipid transfer and metabolic reprogramming in a ciliate–algal symbiosis Article Snippet: P. bursaria cells were fed with 0.1mM 13C-labeled oleic acid (13C18-oleic acid, Article Title: Host-derived lipid transfer and metabolic reprogramming in a ciliate–algal symbiosis Article Snippet: PrePrint: for MS1 and 0.05 Da for MS/MS, with a retention-time tolerance of 0.5 min for lipids. A score cutoff of 75 was applied. P. bursaria cells were fed with 0.1mM 13C-labeled oleic acid (13C18-oleic acid, Capsules:Article Title: Host-derived lipid transfer and metabolic reprogramming in a ciliate–algal symbiosis Article Snippet: P. bursaria cells were fed with 0.1mM 13C-labeled oleic acid (13C18-oleic acid, Article Title: Host-derived lipid transfer and metabolic reprogramming in a ciliate–algal symbiosis Article Snippet: PrePrint: for MS1 and 0.05 Da for MS/MS, with a retention-time tolerance of 0.5 min for lipids. A score cutoff of 75 was applied. P. bursaria cells were fed with 0.1mM 13C-labeled oleic acid (13C18-oleic acid, Algae:Article Title: Host-derived lipid transfer and metabolic reprogramming in a ciliate–algal symbiosis Article Snippet: P. bursaria cells were fed with 0.1mM 13C-labeled oleic acid (13C18-oleic acid, Article Title: Host-derived lipid transfer and metabolic reprogramming in a ciliate–algal symbiosis Article Snippet: PrePrint: for MS1 and 0.05 Da for MS/MS, with a retention-time tolerance of 0.5 min for lipids. A score cutoff of 75 was applied. P. bursaria cells were fed with 0.1mM 13C-labeled oleic acid (13C18-oleic acid, |