tbms1  (MedChemExpress)

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Tubeimoside I Ameliorates Doxorubicin-Induced Cardiotoxicity by Upregulating SIRT3

doi: 10.1155/2023/9966355

Figure Lengend Snippet: TBM alleviated DOX-induced cellular injury in H9c2 cells. (a, b) The chemical structures of DOX and TBM, respectively. (c, d) Viability of H9c2 cells treated with DOX and TBM for 24 h ( n = 6). (e) TBM improved the viability of DOX treated H9c2 cells ( n = 6), ns (not significant) versus the DOX group. (f–h) Levels of LDH, cTNT, and CK-MB in H9c2 cell media ( n = 4), ns (not significant) versus the CON group. Values indicate the mean ± standard deviation (SD). ∗ P < 0.05 versus the CON group and the TBM group; # P < 0.05 versus the DOX group.

Article Snippet: DOX (#HY15142), TBM (#HY-N0890), and 3-(1H-1,2,3-triazol-4-yl) pyridine (3-TYP, #HY108331) were purchased from MedChemExpress (New Jersey, USA); Dihydroethidium (DHE, #D1008) was obtained from Bioscience (Shanghai, China); the TdT-mediated dUTP nick end-labeling (TUNEL) assay kit (#C1088), Annexin V-FITC/PI (propidium iodide) apoptosis detection kit ( #C1062), Nuclear Protein Extraction Kit (#P0027), and mitochondrial membrane potential assay kit with JC-1 (#C2006) were purchased from Beyotime Biotechnology (Shanghai, China); assay kits for detecting lactate dehydrogenase (LDH, #A02022), creatine kinase isoenzymes (CK-MB, #E00611), cardiac isoform of Troponin T (cTnT, H1494), superoxide dismutase (SOD, #A0011) activity, glutathione peroxidase (GSH-PX, #A005), and malondialdehyde (MDA, #A0031) were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China); the cell counting kit-8 (CCK8) was obtained from TopScience (Shanghai, China).

Techniques: Standard Deviation

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Tubeimoside I Ameliorates Doxorubicin-Induced Cardiotoxicity by Upregulating SIRT3

doi: 10.1155/2023/9966355

Figure Lengend Snippet: TBM attenuated inflammation in response to DOX treatment in vitro. (a–c) The relative mRNA levels of Il-6, Il-1 β , and Tnf -α in DOX treated H9c2 cells ( n = 5). (d–h) Western blotting assays of IL-6, IL-1 β , and TNF- α in each group ( n = 3). (i–k) Levels of IL-6, IL-1 β , and TNF- α in cell supernatant determined by ELISA ( n = 4). Values indicate the mean ± SD. ∗ P < 0.05 versus the CON group and the TBM group; # P < 0.05 versus the DOX group; ns (not significant) versus the CON group.

Article Snippet: DOX (#HY15142), TBM (#HY-N0890), and 3-(1H-1,2,3-triazol-4-yl) pyridine (3-TYP, #HY108331) were purchased from MedChemExpress (New Jersey, USA); Dihydroethidium (DHE, #D1008) was obtained from Bioscience (Shanghai, China); the TdT-mediated dUTP nick end-labeling (TUNEL) assay kit (#C1088), Annexin V-FITC/PI (propidium iodide) apoptosis detection kit ( #C1062), Nuclear Protein Extraction Kit (#P0027), and mitochondrial membrane potential assay kit with JC-1 (#C2006) were purchased from Beyotime Biotechnology (Shanghai, China); assay kits for detecting lactate dehydrogenase (LDH, #A02022), creatine kinase isoenzymes (CK-MB, #E00611), cardiac isoform of Troponin T (cTnT, H1494), superoxide dismutase (SOD, #A0011) activity, glutathione peroxidase (GSH-PX, #A005), and malondialdehyde (MDA, #A0031) were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China); the cell counting kit-8 (CCK8) was obtained from TopScience (Shanghai, China).

Techniques: In Vitro, Western Blot, Enzyme-linked Immunosorbent Assay

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Tubeimoside I Ameliorates Doxorubicin-Induced Cardiotoxicity by Upregulating SIRT3

doi: 10.1155/2023/9966355

Figure Lengend Snippet: TBM remarkably ameliorated DOX-evoked oxidative stress in H9c2 cells. (a, b) Flow cytometry was used to estimate the ROS levels in H9c2 cells ( n = 4). (c–e) The level of MDA and the activity of SOD and GSH-PX in H9c2 cells were detected by commercial kits ( n = 4). (f–h) The mRNA levels of Nrf2, Ho-1, and Nqo1 in H9c2 cells determined by qPCR ( n = 5). (i–m) Representative western blotting results for Nrf2, HO-1, and NQO1 in vitro ( n = 3). Values indicate the mean ± SD. ∗ P < 0.05 versus the CON group and the TBM group; # P < 0.05 versus the DOX group; ns (not significant) versus the CON group.

Article Snippet: DOX (#HY15142), TBM (#HY-N0890), and 3-(1H-1,2,3-triazol-4-yl) pyridine (3-TYP, #HY108331) were purchased from MedChemExpress (New Jersey, USA); Dihydroethidium (DHE, #D1008) was obtained from Bioscience (Shanghai, China); the TdT-mediated dUTP nick end-labeling (TUNEL) assay kit (#C1088), Annexin V-FITC/PI (propidium iodide) apoptosis detection kit ( #C1062), Nuclear Protein Extraction Kit (#P0027), and mitochondrial membrane potential assay kit with JC-1 (#C2006) were purchased from Beyotime Biotechnology (Shanghai, China); assay kits for detecting lactate dehydrogenase (LDH, #A02022), creatine kinase isoenzymes (CK-MB, #E00611), cardiac isoform of Troponin T (cTnT, H1494), superoxide dismutase (SOD, #A0011) activity, glutathione peroxidase (GSH-PX, #A005), and malondialdehyde (MDA, #A0031) were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China); the cell counting kit-8 (CCK8) was obtained from TopScience (Shanghai, China).

Techniques: Flow Cytometry, Activity Assay, Western Blot, In Vitro

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Tubeimoside I Ameliorates Doxorubicin-Induced Cardiotoxicity by Upregulating SIRT3

doi: 10.1155/2023/9966355

Figure Lengend Snippet: TBM protected H9c2 cells from DOX-induced apoptosis. (a, b) TUNEL staining labeled DNA breaks in H9c2 cells and its statistical analysis ( n = 3). (c, d) Flow cytometry was also used to evaluate the apoptosis of H9c2 cells ( n = 6). (e, f) Mitochondrial membrane potential (MMP) was determined by JC-1staining ( n = 3). (g, h) Flow cytometry was used to estimate MMP in each group ( n = 3). (i, j) Bax and Bcl-2 mRNA levels tested by qPCR ( n = 5). (k–o) The expression of BAX, BCL-2, and C-caspase3 at protein level determined by western blotting in vitro ( n = 3). Values indicate the mean ± SD. ∗ P < 0.05 versus the CON group and the TBM group; # P < 0.05 versus the DOX group; ns (not significant) versus the CON group.

Article Snippet: DOX (#HY15142), TBM (#HY-N0890), and 3-(1H-1,2,3-triazol-4-yl) pyridine (3-TYP, #HY108331) were purchased from MedChemExpress (New Jersey, USA); Dihydroethidium (DHE, #D1008) was obtained from Bioscience (Shanghai, China); the TdT-mediated dUTP nick end-labeling (TUNEL) assay kit (#C1088), Annexin V-FITC/PI (propidium iodide) apoptosis detection kit ( #C1062), Nuclear Protein Extraction Kit (#P0027), and mitochondrial membrane potential assay kit with JC-1 (#C2006) were purchased from Beyotime Biotechnology (Shanghai, China); assay kits for detecting lactate dehydrogenase (LDH, #A02022), creatine kinase isoenzymes (CK-MB, #E00611), cardiac isoform of Troponin T (cTnT, H1494), superoxide dismutase (SOD, #A0011) activity, glutathione peroxidase (GSH-PX, #A005), and malondialdehyde (MDA, #A0031) were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China); the cell counting kit-8 (CCK8) was obtained from TopScience (Shanghai, China).

Techniques: TUNEL Assay, Staining, Labeling, Flow Cytometry, Membrane, Expressing, Western Blot, In Vitro

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Tubeimoside I Ameliorates Doxorubicin-Induced Cardiotoxicity by Upregulating SIRT3

doi: 10.1155/2023/9966355

Figure Lengend Snippet: TBM alleviated DOX-induced cardiotoxicity in mice. (a) Representative images of M-mode echocardiography in each group ( n = 6). (b, c) Statistical analysis of left ventricular ejection fraction (EF) and fractional shortening (FS) ( n = 6). (d) Heart rate of mice ( n = 6). (e) HE staining of mouse hearts ( n = 6). (f) The body weight changes in each group were recorded every other day ( n = 6). (g–i) The serum levels of LDH, cTNT, and CK-MB in mice ( n = 6). Values indicate the mean ± SD. ∗ P < 0.05 versus the CON group and the TBM group; # P < 0.05 versus the DOX group; ns (not significant) versus the CON group.

Article Snippet: DOX (#HY15142), TBM (#HY-N0890), and 3-(1H-1,2,3-triazol-4-yl) pyridine (3-TYP, #HY108331) were purchased from MedChemExpress (New Jersey, USA); Dihydroethidium (DHE, #D1008) was obtained from Bioscience (Shanghai, China); the TdT-mediated dUTP nick end-labeling (TUNEL) assay kit (#C1088), Annexin V-FITC/PI (propidium iodide) apoptosis detection kit ( #C1062), Nuclear Protein Extraction Kit (#P0027), and mitochondrial membrane potential assay kit with JC-1 (#C2006) were purchased from Beyotime Biotechnology (Shanghai, China); assay kits for detecting lactate dehydrogenase (LDH, #A02022), creatine kinase isoenzymes (CK-MB, #E00611), cardiac isoform of Troponin T (cTnT, H1494), superoxide dismutase (SOD, #A0011) activity, glutathione peroxidase (GSH-PX, #A005), and malondialdehyde (MDA, #A0031) were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China); the cell counting kit-8 (CCK8) was obtained from TopScience (Shanghai, China).

Techniques: Staining

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Tubeimoside I Ameliorates Doxorubicin-Induced Cardiotoxicity by Upregulating SIRT3

doi: 10.1155/2023/9966355

Figure Lengend Snippet: TBM inhibited DOX-induced inflammatory response in vivo. (a–c) The relative mRNA levels of Il-6, Il-1 β , and Tnf -α in mouse hearts (n = 6). (d–h) Western blotting for the detection of NF- κ B P65 and inflammatory factors (IL-6, IL-1 β , and TNF- α ) in mice ( n = 6). (i–k) The levels of IL-6, IL-1 β , and TNF- α in mouse hearts determined by ELISA ( n = 6). Values indicate the mean ± SD. ∗ P < 0.05 versus the CON group and the TBM group; # P < 0.05 versus the DOX group; ns (not significant) versus the CON group.

Article Snippet: DOX (#HY15142), TBM (#HY-N0890), and 3-(1H-1,2,3-triazol-4-yl) pyridine (3-TYP, #HY108331) were purchased from MedChemExpress (New Jersey, USA); Dihydroethidium (DHE, #D1008) was obtained from Bioscience (Shanghai, China); the TdT-mediated dUTP nick end-labeling (TUNEL) assay kit (#C1088), Annexin V-FITC/PI (propidium iodide) apoptosis detection kit ( #C1062), Nuclear Protein Extraction Kit (#P0027), and mitochondrial membrane potential assay kit with JC-1 (#C2006) were purchased from Beyotime Biotechnology (Shanghai, China); assay kits for detecting lactate dehydrogenase (LDH, #A02022), creatine kinase isoenzymes (CK-MB, #E00611), cardiac isoform of Troponin T (cTnT, H1494), superoxide dismutase (SOD, #A0011) activity, glutathione peroxidase (GSH-PX, #A005), and malondialdehyde (MDA, #A0031) were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China); the cell counting kit-8 (CCK8) was obtained from TopScience (Shanghai, China).

Techniques: In Vivo, Western Blot, Enzyme-linked Immunosorbent Assay

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Tubeimoside I Ameliorates Doxorubicin-Induced Cardiotoxicity by Upregulating SIRT3

doi: 10.1155/2023/9966355

Figure Lengend Snippet: TBM protected against DOX-induced oxidative stress in mice. (a, b) DHE staining indicated the generation of ROS in mice hearts ( n = 6). (c) The MDA levels in mouse hearts ( n = 6). (d, e) The activity of SOD and GSH-PX in mouse hearts ( n = 6). (f–h) The mRNA levels of Nrf2, Ho-1, and Nqo1 in mice determined by qPCR ( n = 6). (i–m) Western blotting analysis of Nrf2, HO-1, and NQO1 at the protein level ( n = 6). Values indicate the mean ± SD. ∗ P < 0.05 versus the CON group and the TBM group; # P < 0.05 versus the DOX group; ns (not significant) versus the CON group.

Article Snippet: DOX (#HY15142), TBM (#HY-N0890), and 3-(1H-1,2,3-triazol-4-yl) pyridine (3-TYP, #HY108331) were purchased from MedChemExpress (New Jersey, USA); Dihydroethidium (DHE, #D1008) was obtained from Bioscience (Shanghai, China); the TdT-mediated dUTP nick end-labeling (TUNEL) assay kit (#C1088), Annexin V-FITC/PI (propidium iodide) apoptosis detection kit ( #C1062), Nuclear Protein Extraction Kit (#P0027), and mitochondrial membrane potential assay kit with JC-1 (#C2006) were purchased from Beyotime Biotechnology (Shanghai, China); assay kits for detecting lactate dehydrogenase (LDH, #A02022), creatine kinase isoenzymes (CK-MB, #E00611), cardiac isoform of Troponin T (cTnT, H1494), superoxide dismutase (SOD, #A0011) activity, glutathione peroxidase (GSH-PX, #A005), and malondialdehyde (MDA, #A0031) were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China); the cell counting kit-8 (CCK8) was obtained from TopScience (Shanghai, China).

Techniques: Staining, Activity Assay, Western Blot

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Tubeimoside I Ameliorates Doxorubicin-Induced Cardiotoxicity by Upregulating SIRT3

doi: 10.1155/2023/9966355

Figure Lengend Snippet: TBM mitigated DOX-provoked cardiomyocyte apoptosis in vivo. (a, b) TUNEL staining represents cardiomyocyte apoptosis in the mouse hearts and its statistical result ( n = 6). (c, d) The mRNA levels of Bax and Bcl-2 determined by qPCR analysis ( n = 6). (e–i) Western blotting assays for the detection of BAX, BCL-2, and C-Caspase3 expression ( n = 6). Values indicate the mean ± SD. ∗ P < 0.05 versus the CON group and the TBM group; # P < 0.05 versus the DOX group; ns (not significant) versus the CON group.

Article Snippet: DOX (#HY15142), TBM (#HY-N0890), and 3-(1H-1,2,3-triazol-4-yl) pyridine (3-TYP, #HY108331) were purchased from MedChemExpress (New Jersey, USA); Dihydroethidium (DHE, #D1008) was obtained from Bioscience (Shanghai, China); the TdT-mediated dUTP nick end-labeling (TUNEL) assay kit (#C1088), Annexin V-FITC/PI (propidium iodide) apoptosis detection kit ( #C1062), Nuclear Protein Extraction Kit (#P0027), and mitochondrial membrane potential assay kit with JC-1 (#C2006) were purchased from Beyotime Biotechnology (Shanghai, China); assay kits for detecting lactate dehydrogenase (LDH, #A02022), creatine kinase isoenzymes (CK-MB, #E00611), cardiac isoform of Troponin T (cTnT, H1494), superoxide dismutase (SOD, #A0011) activity, glutathione peroxidase (GSH-PX, #A005), and malondialdehyde (MDA, #A0031) were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China); the cell counting kit-8 (CCK8) was obtained from TopScience (Shanghai, China).

Techniques: In Vivo, TUNEL Assay, Staining, Western Blot, Expressing

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Tubeimoside I Ameliorates Doxorubicin-Induced Cardiotoxicity by Upregulating SIRT3

doi: 10.1155/2023/9966355

Figure Lengend Snippet: TBM prevented DOX-evoked cellular damage by upregulating SIRT3 in H9c2 cells. (a, b) si-SIRT3 inhibited the expression of SIRT3 as determined by western blotting (n = 4). (c–h) The expression of SIRT3, P-P65, T-P65, IL-6, IL-1 β , and TNF- α was estimated by western blotting in H9c2 cells ( n = 3). (i–m) Western blotting was used to detect the expression of Nrf2, HO-1, and NQO1 in vitro ( n = 3). (n, p) si-Sirt3 partially reversed the inhibitory roles of TBM in DOX-induced ROS production ( n = 4). (o, q) Flow cytometry analysis demonstrated that SIRT3 inhibition blunted the beneficial effects of TBM on DOX-evoked apoptosis ( n = 6). (r–v) Western blotting was used to test the expression of apoptosis related proteins (BAX, BCL-2, and C-Caspase3) in H9c2 cells after si-Sirt3 treatment ( n = 3). Values indicate the mean ± SD. ∗ P < 0.05 versus the CON group and the TBM group; # P < 0.05 versus the DOX group; ns (not significant) versus the CON group; & P < 0.05 versus the DOX + TBM + siSIRT3 group.

Article Snippet: DOX (#HY15142), TBM (#HY-N0890), and 3-(1H-1,2,3-triazol-4-yl) pyridine (3-TYP, #HY108331) were purchased from MedChemExpress (New Jersey, USA); Dihydroethidium (DHE, #D1008) was obtained from Bioscience (Shanghai, China); the TdT-mediated dUTP nick end-labeling (TUNEL) assay kit (#C1088), Annexin V-FITC/PI (propidium iodide) apoptosis detection kit ( #C1062), Nuclear Protein Extraction Kit (#P0027), and mitochondrial membrane potential assay kit with JC-1 (#C2006) were purchased from Beyotime Biotechnology (Shanghai, China); assay kits for detecting lactate dehydrogenase (LDH, #A02022), creatine kinase isoenzymes (CK-MB, #E00611), cardiac isoform of Troponin T (cTnT, H1494), superoxide dismutase (SOD, #A0011) activity, glutathione peroxidase (GSH-PX, #A005), and malondialdehyde (MDA, #A0031) were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China); the cell counting kit-8 (CCK8) was obtained from TopScience (Shanghai, China).

Techniques: Expressing, Western Blot, In Vitro, Flow Cytometry, Inhibition

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Tubeimoside I Ameliorates Doxorubicin-Induced Cardiotoxicity by Upregulating SIRT3

doi: 10.1155/2023/9966355

Figure Lengend Snippet: SIRT3 inhibition alleviated the beneficial effects of TBM on DOX-induced cardiac injury in mice. (a, b) 3-TYP inhibited the expression of SIRT3 in mouse hearts ( n = 6). (c–h) The expression of SIRT3, P-P65, T-P65, IL-6, IL-1 β , and TNF- α evaluated by western blotting in mice ( n = 6). (i–m) Western blotting was used to examine the expression of Nrf2, HO-1, and NQO1 in mouse hearts ( n = 6). (n, o) DHE staining illustrated the generation of ROS after SIRT3 inhibition in mouse hearts ( n = 6). (p–s) Western blotting was used to detect the expression of the antiapoptotic protein BCL-2 and proapoptotic proteins (BAX and C-caspase3) in vivo ( n = 6). Values indicate the mean ± SD. ∗ P < 0.05 versus the CON group and the TBM group; # P < 0.05 versus the DOX group; ns (not significant) versus the CON group; & P < 0.05 versus the DOX + TBM+3-TYP group.

Article Snippet: DOX (#HY15142), TBM (#HY-N0890), and 3-(1H-1,2,3-triazol-4-yl) pyridine (3-TYP, #HY108331) were purchased from MedChemExpress (New Jersey, USA); Dihydroethidium (DHE, #D1008) was obtained from Bioscience (Shanghai, China); the TdT-mediated dUTP nick end-labeling (TUNEL) assay kit (#C1088), Annexin V-FITC/PI (propidium iodide) apoptosis detection kit ( #C1062), Nuclear Protein Extraction Kit (#P0027), and mitochondrial membrane potential assay kit with JC-1 (#C2006) were purchased from Beyotime Biotechnology (Shanghai, China); assay kits for detecting lactate dehydrogenase (LDH, #A02022), creatine kinase isoenzymes (CK-MB, #E00611), cardiac isoform of Troponin T (cTnT, H1494), superoxide dismutase (SOD, #A0011) activity, glutathione peroxidase (GSH-PX, #A005), and malondialdehyde (MDA, #A0031) were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China); the cell counting kit-8 (CCK8) was obtained from TopScience (Shanghai, China).

Techniques: Inhibition, Expressing, Western Blot, Staining, In Vivo