High Performance Liquid Chromatography:Article Title: Procyanidin B1 Promotes PSMC3-NRF2 Ubiquitination to Induce Ferroptosis in Glioblastoma.
Article Snippet: Antibodies used included anti- NRF2 (Abcam; cat, ab31163; lot, GR3248767), anti- NRF2 (Proteintech; cat, 16396- 1- AP; lot, 2354154), anti- KI- 67 (Abcam; cat, ab15580; lot, GR3362242), anti- SLC7A11 (MedChemExpress; cat, YA006; lot, 267113), anti- COX2 (Abcam; cat, ab179800; lot, GR222252), and antiTFR1 (Abcam; cat, ab214039; lot, GR3235217). .. Reagents used included erastin (MedChemExpress, HY- 15763), ferrostatin- 1 (MedChemExpress, HY- 100579), procyanidin B1 (Meilunbio, MB7155- 1, HPLC ≥95%), temozolomide (TMZ; MedChemExpress, HY- 17364), Cell counting kit- 8 (CCK- 8; MedChemExpress, HY- K0301), Lipofectamine 3000 transfection kit (Thermo, L3000001), calcein/ propidium iodide (PI) cell viability/cytotoxicity assay kit (Beyotime, C2015S), DMEM medium (Gibco, 11965092), FBS (Gibco, A5670701), penicillin and streptomycin (Solarbio, P1400), mRNA reverse transcription kit (Thermo Scientific, K16225), TRIzol reagent kit (Invitrogen, 15596018CN), Amplex Red kit (Beyotime, amplex red kit), Protein A/G Magnetic Beads (MedChemExpress, HYK0202), Opti- MEM medium (Gibco, 31985070), lipid peroxide sensor (LPS; MKBIO, 217075- 36- 0), NADPH assay kit with WST- 8 (Beyotime, S0179), GSH assay kit (Beyotime, S0053), bicinchoninic acid kit (Thermo Fisher Scientific, 23222), Iron ion detection kit (Leagene, TC1015), D- luciferin, sodium salt (Meilunbio, MB1837), radioimmunoprecipitation assay (RIPA) buffer (Solarbio, R0020), Proease inhibitor cocktail (Solarbio, A8260), and NRF2 protein (MedChemExpress, HY- P72308). .. An in vitro BBB model was constructed using six- well transwell plates (Corning, Inc. Corning, NY, USA) using the bEnd.3 cell line.
Article Title: Procyanidin B1 Promotes PSMC3‐NRF2 Ubiquitination to Induce Ferroptosis in Glioblastoma
Article Snippet: Antibodies used included anti‐NRF2 (Abcam; cat, ab31163; lot, GR3248767), anti‐NRF2 (Proteintech; cat, 16396‐1‐AP; lot, 2354154), anti‐KI‐67 (Abcam; cat, ab15580; lot, GR3362242), anti‐SLC7A11 (MedChemExpress; cat, YA006; lot, 267113), anti‐COX2 (Abcam; cat, ab179800; lot, GR222252), and anti‐TFR1 (Abcam; cat, ab214039; lot, GR3235217). .. Reagents used included erastin (MedChemExpress, HY‐15763), ferrostatin‐1 (MedChemExpress, HY‐100579), procyanidin B1 (Meilunbio, MB7155‐1, HPLC ≥95%), temozolomide (TMZ; MedChemExpress, HY‐17364), Cell counting kit‐8 (CCK‐8; MedChemExpress, HY‐K0301), Lipofectamine 3000 transfection kit (Thermo, L3000001), calcein/ propidium iodide (PI) cell viability/cytotoxicity assay kit (Beyotime, C2015S), DMEM medium (Gibco, 11965092), FBS (Gibco, A5670701), penicillin and streptomycin (Solarbio, P1400), mRNA reverse transcription kit (Thermo Scientific, K16225), TRIzol reagent kit (Invitrogen, 15596018CN), Amplex Red kit (Beyotime, amplex red kit), Protein A/G Magnetic Beads (MedChemExpress, HY‐K0202), Opti‐MEM medium (Gibco, 31985070), lipid peroxide sensor (LPS; MKBIO, 217075‐36‐0), NADPH assay kit with WST‐8 (Beyotime, S0179), GSH assay kit (Beyotime, S0053), bicinchoninic acid kit (Thermo Fisher Scientific, 23222), Iron ion detection kit (Leagene, TC1015), D‐luciferin, sodium salt (Meilunbio, MB1837), radioimmunoprecipitation assay (RIPA) buffer (Solarbio, R0020), Proease inhibitor cocktail (Solarbio, A8260), and NRF2 protein (MedChemExpress, HY‐P72308). .. An in vitro BBB model was constructed using six‐well transwell plates (Corning, Inc. Corning, NY, USA) using the bEnd.3 cell line.
Cell Counting:Article Title: Procyanidin B1 Promotes PSMC3-NRF2 Ubiquitination to Induce Ferroptosis in Glioblastoma.
Article Snippet: Antibodies used included anti- NRF2 (Abcam; cat, ab31163; lot, GR3248767), anti- NRF2 (Proteintech; cat, 16396- 1- AP; lot, 2354154), anti- KI- 67 (Abcam; cat, ab15580; lot, GR3362242), anti- SLC7A11 (MedChemExpress; cat, YA006; lot, 267113), anti- COX2 (Abcam; cat, ab179800; lot, GR222252), and antiTFR1 (Abcam; cat, ab214039; lot, GR3235217). .. Reagents used included erastin (MedChemExpress, HY- 15763), ferrostatin- 1 (MedChemExpress, HY- 100579), procyanidin B1 (Meilunbio, MB7155- 1, HPLC ≥95%), temozolomide (TMZ; MedChemExpress, HY- 17364), Cell counting kit- 8 (CCK- 8; MedChemExpress, HY- K0301), Lipofectamine 3000 transfection kit (Thermo, L3000001), calcein/ propidium iodide (PI) cell viability/cytotoxicity assay kit (Beyotime, C2015S), DMEM medium (Gibco, 11965092), FBS (Gibco, A5670701), penicillin and streptomycin (Solarbio, P1400), mRNA reverse transcription kit (Thermo Scientific, K16225), TRIzol reagent kit (Invitrogen, 15596018CN), Amplex Red kit (Beyotime, amplex red kit), Protein A/G Magnetic Beads (MedChemExpress, HYK0202), Opti- MEM medium (Gibco, 31985070), lipid peroxide sensor (LPS; MKBIO, 217075- 36- 0), NADPH assay kit with WST- 8 (Beyotime, S0179), GSH assay kit (Beyotime, S0053), bicinchoninic acid kit (Thermo Fisher Scientific, 23222), Iron ion detection kit (Leagene, TC1015), D- luciferin, sodium salt (Meilunbio, MB1837), radioimmunoprecipitation assay (RIPA) buffer (Solarbio, R0020), Proease inhibitor cocktail (Solarbio, A8260), and NRF2 protein (MedChemExpress, HY- P72308). .. An in vitro BBB model was constructed using six- well transwell plates (Corning, Inc. Corning, NY, USA) using the bEnd.3 cell line.
CCK-8 Assay:Article Title: Procyanidin B1 Promotes PSMC3-NRF2 Ubiquitination to Induce Ferroptosis in Glioblastoma.
Article Snippet: Antibodies used included anti- NRF2 (Abcam; cat, ab31163; lot, GR3248767), anti- NRF2 (Proteintech; cat, 16396- 1- AP; lot, 2354154), anti- KI- 67 (Abcam; cat, ab15580; lot, GR3362242), anti- SLC7A11 (MedChemExpress; cat, YA006; lot, 267113), anti- COX2 (Abcam; cat, ab179800; lot, GR222252), and antiTFR1 (Abcam; cat, ab214039; lot, GR3235217). .. Reagents used included erastin (MedChemExpress, HY- 15763), ferrostatin- 1 (MedChemExpress, HY- 100579), procyanidin B1 (Meilunbio, MB7155- 1, HPLC ≥95%), temozolomide (TMZ; MedChemExpress, HY- 17364), Cell counting kit- 8 (CCK- 8; MedChemExpress, HY- K0301), Lipofectamine 3000 transfection kit (Thermo, L3000001), calcein/ propidium iodide (PI) cell viability/cytotoxicity assay kit (Beyotime, C2015S), DMEM medium (Gibco, 11965092), FBS (Gibco, A5670701), penicillin and streptomycin (Solarbio, P1400), mRNA reverse transcription kit (Thermo Scientific, K16225), TRIzol reagent kit (Invitrogen, 15596018CN), Amplex Red kit (Beyotime, amplex red kit), Protein A/G Magnetic Beads (MedChemExpress, HYK0202), Opti- MEM medium (Gibco, 31985070), lipid peroxide sensor (LPS; MKBIO, 217075- 36- 0), NADPH assay kit with WST- 8 (Beyotime, S0179), GSH assay kit (Beyotime, S0053), bicinchoninic acid kit (Thermo Fisher Scientific, 23222), Iron ion detection kit (Leagene, TC1015), D- luciferin, sodium salt (Meilunbio, MB1837), radioimmunoprecipitation assay (RIPA) buffer (Solarbio, R0020), Proease inhibitor cocktail (Solarbio, A8260), and NRF2 protein (MedChemExpress, HY- P72308). .. An in vitro BBB model was constructed using six- well transwell plates (Corning, Inc. Corning, NY, USA) using the bEnd.3 cell line.
Article Title: Procyanidin B1 Promotes PSMC3‐NRF2 Ubiquitination to Induce Ferroptosis in Glioblastoma
Article Snippet: Antibodies used included anti‐NRF2 (Abcam; cat, ab31163; lot, GR3248767), anti‐NRF2 (Proteintech; cat, 16396‐1‐AP; lot, 2354154), anti‐KI‐67 (Abcam; cat, ab15580; lot, GR3362242), anti‐SLC7A11 (MedChemExpress; cat, YA006; lot, 267113), anti‐COX2 (Abcam; cat, ab179800; lot, GR222252), and anti‐TFR1 (Abcam; cat, ab214039; lot, GR3235217). .. Reagents used included erastin (MedChemExpress, HY‐15763), ferrostatin‐1 (MedChemExpress, HY‐100579), procyanidin B1 (Meilunbio, MB7155‐1, HPLC ≥95%), temozolomide (TMZ; MedChemExpress, HY‐17364), Cell counting kit‐8 (CCK‐8; MedChemExpress, HY‐K0301), Lipofectamine 3000 transfection kit (Thermo, L3000001), calcein/ propidium iodide (PI) cell viability/cytotoxicity assay kit (Beyotime, C2015S), DMEM medium (Gibco, 11965092), FBS (Gibco, A5670701), penicillin and streptomycin (Solarbio, P1400), mRNA reverse transcription kit (Thermo Scientific, K16225), TRIzol reagent kit (Invitrogen, 15596018CN), Amplex Red kit (Beyotime, amplex red kit), Protein A/G Magnetic Beads (MedChemExpress, HY‐K0202), Opti‐MEM medium (Gibco, 31985070), lipid peroxide sensor (LPS; MKBIO, 217075‐36‐0), NADPH assay kit with WST‐8 (Beyotime, S0179), GSH assay kit (Beyotime, S0053), bicinchoninic acid kit (Thermo Fisher Scientific, 23222), Iron ion detection kit (Leagene, TC1015), D‐luciferin, sodium salt (Meilunbio, MB1837), radioimmunoprecipitation assay (RIPA) buffer (Solarbio, R0020), Proease inhibitor cocktail (Solarbio, A8260), and NRF2 protein (MedChemExpress, HY‐P72308). .. An in vitro BBB model was constructed using six‐well transwell plates (Corning, Inc. Corning, NY, USA) using the bEnd.3 cell line.
Transfection:Article Title: Procyanidin B1 Promotes PSMC3-NRF2 Ubiquitination to Induce Ferroptosis in Glioblastoma.
Article Snippet: Antibodies used included anti- NRF2 (Abcam; cat, ab31163; lot, GR3248767), anti- NRF2 (Proteintech; cat, 16396- 1- AP; lot, 2354154), anti- KI- 67 (Abcam; cat, ab15580; lot, GR3362242), anti- SLC7A11 (MedChemExpress; cat, YA006; lot, 267113), anti- COX2 (Abcam; cat, ab179800; lot, GR222252), and antiTFR1 (Abcam; cat, ab214039; lot, GR3235217). .. Reagents used included erastin (MedChemExpress, HY- 15763), ferrostatin- 1 (MedChemExpress, HY- 100579), procyanidin B1 (Meilunbio, MB7155- 1, HPLC ≥95%), temozolomide (TMZ; MedChemExpress, HY- 17364), Cell counting kit- 8 (CCK- 8; MedChemExpress, HY- K0301), Lipofectamine 3000 transfection kit (Thermo, L3000001), calcein/ propidium iodide (PI) cell viability/cytotoxicity assay kit (Beyotime, C2015S), DMEM medium (Gibco, 11965092), FBS (Gibco, A5670701), penicillin and streptomycin (Solarbio, P1400), mRNA reverse transcription kit (Thermo Scientific, K16225), TRIzol reagent kit (Invitrogen, 15596018CN), Amplex Red kit (Beyotime, amplex red kit), Protein A/G Magnetic Beads (MedChemExpress, HYK0202), Opti- MEM medium (Gibco, 31985070), lipid peroxide sensor (LPS; MKBIO, 217075- 36- 0), NADPH assay kit with WST- 8 (Beyotime, S0179), GSH assay kit (Beyotime, S0053), bicinchoninic acid kit (Thermo Fisher Scientific, 23222), Iron ion detection kit (Leagene, TC1015), D- luciferin, sodium salt (Meilunbio, MB1837), radioimmunoprecipitation assay (RIPA) buffer (Solarbio, R0020), Proease inhibitor cocktail (Solarbio, A8260), and NRF2 protein (MedChemExpress, HY- P72308). .. An in vitro BBB model was constructed using six- well transwell plates (Corning, Inc. Corning, NY, USA) using the bEnd.3 cell line.
Article Title: Procyanidin B1 Promotes PSMC3‐NRF2 Ubiquitination to Induce Ferroptosis in Glioblastoma
Article Snippet: Antibodies used included anti‐NRF2 (Abcam; cat, ab31163; lot, GR3248767), anti‐NRF2 (Proteintech; cat, 16396‐1‐AP; lot, 2354154), anti‐KI‐67 (Abcam; cat, ab15580; lot, GR3362242), anti‐SLC7A11 (MedChemExpress; cat, YA006; lot, 267113), anti‐COX2 (Abcam; cat, ab179800; lot, GR222252), and anti‐TFR1 (Abcam; cat, ab214039; lot, GR3235217). .. Reagents used included erastin (MedChemExpress, HY‐15763), ferrostatin‐1 (MedChemExpress, HY‐100579), procyanidin B1 (Meilunbio, MB7155‐1, HPLC ≥95%), temozolomide (TMZ; MedChemExpress, HY‐17364), Cell counting kit‐8 (CCK‐8; MedChemExpress, HY‐K0301), Lipofectamine 3000 transfection kit (Thermo, L3000001), calcein/ propidium iodide (PI) cell viability/cytotoxicity assay kit (Beyotime, C2015S), DMEM medium (Gibco, 11965092), FBS (Gibco, A5670701), penicillin and streptomycin (Solarbio, P1400), mRNA reverse transcription kit (Thermo Scientific, K16225), TRIzol reagent kit (Invitrogen, 15596018CN), Amplex Red kit (Beyotime, amplex red kit), Protein A/G Magnetic Beads (MedChemExpress, HY‐K0202), Opti‐MEM medium (Gibco, 31985070), lipid peroxide sensor (LPS; MKBIO, 217075‐36‐0), NADPH assay kit with WST‐8 (Beyotime, S0179), GSH assay kit (Beyotime, S0053), bicinchoninic acid kit (Thermo Fisher Scientific, 23222), Iron ion detection kit (Leagene, TC1015), D‐luciferin, sodium salt (Meilunbio, MB1837), radioimmunoprecipitation assay (RIPA) buffer (Solarbio, R0020), Proease inhibitor cocktail (Solarbio, A8260), and NRF2 protein (MedChemExpress, HY‐P72308). .. An in vitro BBB model was constructed using six‐well transwell plates (Corning, Inc. Corning, NY, USA) using the bEnd.3 cell line.
Reverse Transcription:Article Title: Procyanidin B1 Promotes PSMC3-NRF2 Ubiquitination to Induce Ferroptosis in Glioblastoma.
Article Snippet: Antibodies used included anti- NRF2 (Abcam; cat, ab31163; lot, GR3248767), anti- NRF2 (Proteintech; cat, 16396- 1- AP; lot, 2354154), anti- KI- 67 (Abcam; cat, ab15580; lot, GR3362242), anti- SLC7A11 (MedChemExpress; cat, YA006; lot, 267113), anti- COX2 (Abcam; cat, ab179800; lot, GR222252), and antiTFR1 (Abcam; cat, ab214039; lot, GR3235217). .. Reagents used included erastin (MedChemExpress, HY- 15763), ferrostatin- 1 (MedChemExpress, HY- 100579), procyanidin B1 (Meilunbio, MB7155- 1, HPLC ≥95%), temozolomide (TMZ; MedChemExpress, HY- 17364), Cell counting kit- 8 (CCK- 8; MedChemExpress, HY- K0301), Lipofectamine 3000 transfection kit (Thermo, L3000001), calcein/ propidium iodide (PI) cell viability/cytotoxicity assay kit (Beyotime, C2015S), DMEM medium (Gibco, 11965092), FBS (Gibco, A5670701), penicillin and streptomycin (Solarbio, P1400), mRNA reverse transcription kit (Thermo Scientific, K16225), TRIzol reagent kit (Invitrogen, 15596018CN), Amplex Red kit (Beyotime, amplex red kit), Protein A/G Magnetic Beads (MedChemExpress, HYK0202), Opti- MEM medium (Gibco, 31985070), lipid peroxide sensor (LPS; MKBIO, 217075- 36- 0), NADPH assay kit with WST- 8 (Beyotime, S0179), GSH assay kit (Beyotime, S0053), bicinchoninic acid kit (Thermo Fisher Scientific, 23222), Iron ion detection kit (Leagene, TC1015), D- luciferin, sodium salt (Meilunbio, MB1837), radioimmunoprecipitation assay (RIPA) buffer (Solarbio, R0020), Proease inhibitor cocktail (Solarbio, A8260), and NRF2 protein (MedChemExpress, HY- P72308). .. An in vitro BBB model was constructed using six- well transwell plates (Corning, Inc. Corning, NY, USA) using the bEnd.3 cell line.
Article Title: Procyanidin B1 Promotes PSMC3‐NRF2 Ubiquitination to Induce Ferroptosis in Glioblastoma
Article Snippet: Antibodies used included anti‐NRF2 (Abcam; cat, ab31163; lot, GR3248767), anti‐NRF2 (Proteintech; cat, 16396‐1‐AP; lot, 2354154), anti‐KI‐67 (Abcam; cat, ab15580; lot, GR3362242), anti‐SLC7A11 (MedChemExpress; cat, YA006; lot, 267113), anti‐COX2 (Abcam; cat, ab179800; lot, GR222252), and anti‐TFR1 (Abcam; cat, ab214039; lot, GR3235217). .. Reagents used included erastin (MedChemExpress, HY‐15763), ferrostatin‐1 (MedChemExpress, HY‐100579), procyanidin B1 (Meilunbio, MB7155‐1, HPLC ≥95%), temozolomide (TMZ; MedChemExpress, HY‐17364), Cell counting kit‐8 (CCK‐8; MedChemExpress, HY‐K0301), Lipofectamine 3000 transfection kit (Thermo, L3000001), calcein/ propidium iodide (PI) cell viability/cytotoxicity assay kit (Beyotime, C2015S), DMEM medium (Gibco, 11965092), FBS (Gibco, A5670701), penicillin and streptomycin (Solarbio, P1400), mRNA reverse transcription kit (Thermo Scientific, K16225), TRIzol reagent kit (Invitrogen, 15596018CN), Amplex Red kit (Beyotime, amplex red kit), Protein A/G Magnetic Beads (MedChemExpress, HY‐K0202), Opti‐MEM medium (Gibco, 31985070), lipid peroxide sensor (LPS; MKBIO, 217075‐36‐0), NADPH assay kit with WST‐8 (Beyotime, S0179), GSH assay kit (Beyotime, S0053), bicinchoninic acid kit (Thermo Fisher Scientific, 23222), Iron ion detection kit (Leagene, TC1015), D‐luciferin, sodium salt (Meilunbio, MB1837), radioimmunoprecipitation assay (RIPA) buffer (Solarbio, R0020), Proease inhibitor cocktail (Solarbio, A8260), and NRF2 protein (MedChemExpress, HY‐P72308). .. An in vitro BBB model was constructed using six‐well transwell plates (Corning, Inc. Corning, NY, USA) using the bEnd.3 cell line.
Magnetic Beads:Article Title: Procyanidin B1 Promotes PSMC3-NRF2 Ubiquitination to Induce Ferroptosis in Glioblastoma.
Article Snippet: Antibodies used included anti- NRF2 (Abcam; cat, ab31163; lot, GR3248767), anti- NRF2 (Proteintech; cat, 16396- 1- AP; lot, 2354154), anti- KI- 67 (Abcam; cat, ab15580; lot, GR3362242), anti- SLC7A11 (MedChemExpress; cat, YA006; lot, 267113), anti- COX2 (Abcam; cat, ab179800; lot, GR222252), and antiTFR1 (Abcam; cat, ab214039; lot, GR3235217). .. Reagents used included erastin (MedChemExpress, HY- 15763), ferrostatin- 1 (MedChemExpress, HY- 100579), procyanidin B1 (Meilunbio, MB7155- 1, HPLC ≥95%), temozolomide (TMZ; MedChemExpress, HY- 17364), Cell counting kit- 8 (CCK- 8; MedChemExpress, HY- K0301), Lipofectamine 3000 transfection kit (Thermo, L3000001), calcein/ propidium iodide (PI) cell viability/cytotoxicity assay kit (Beyotime, C2015S), DMEM medium (Gibco, 11965092), FBS (Gibco, A5670701), penicillin and streptomycin (Solarbio, P1400), mRNA reverse transcription kit (Thermo Scientific, K16225), TRIzol reagent kit (Invitrogen, 15596018CN), Amplex Red kit (Beyotime, amplex red kit), Protein A/G Magnetic Beads (MedChemExpress, HYK0202), Opti- MEM medium (Gibco, 31985070), lipid peroxide sensor (LPS; MKBIO, 217075- 36- 0), NADPH assay kit with WST- 8 (Beyotime, S0179), GSH assay kit (Beyotime, S0053), bicinchoninic acid kit (Thermo Fisher Scientific, 23222), Iron ion detection kit (Leagene, TC1015), D- luciferin, sodium salt (Meilunbio, MB1837), radioimmunoprecipitation assay (RIPA) buffer (Solarbio, R0020), Proease inhibitor cocktail (Solarbio, A8260), and NRF2 protein (MedChemExpress, HY- P72308). .. An in vitro BBB model was constructed using six- well transwell plates (Corning, Inc. Corning, NY, USA) using the bEnd.3 cell line.
Article Title: Procyanidin B1 Promotes PSMC3‐NRF2 Ubiquitination to Induce Ferroptosis in Glioblastoma
Article Snippet: Antibodies used included anti‐NRF2 (Abcam; cat, ab31163; lot, GR3248767), anti‐NRF2 (Proteintech; cat, 16396‐1‐AP; lot, 2354154), anti‐KI‐67 (Abcam; cat, ab15580; lot, GR3362242), anti‐SLC7A11 (MedChemExpress; cat, YA006; lot, 267113), anti‐COX2 (Abcam; cat, ab179800; lot, GR222252), and anti‐TFR1 (Abcam; cat, ab214039; lot, GR3235217). .. Reagents used included erastin (MedChemExpress, HY‐15763), ferrostatin‐1 (MedChemExpress, HY‐100579), procyanidin B1 (Meilunbio, MB7155‐1, HPLC ≥95%), temozolomide (TMZ; MedChemExpress, HY‐17364), Cell counting kit‐8 (CCK‐8; MedChemExpress, HY‐K0301), Lipofectamine 3000 transfection kit (Thermo, L3000001), calcein/ propidium iodide (PI) cell viability/cytotoxicity assay kit (Beyotime, C2015S), DMEM medium (Gibco, 11965092), FBS (Gibco, A5670701), penicillin and streptomycin (Solarbio, P1400), mRNA reverse transcription kit (Thermo Scientific, K16225), TRIzol reagent kit (Invitrogen, 15596018CN), Amplex Red kit (Beyotime, amplex red kit), Protein A/G Magnetic Beads (MedChemExpress, HY‐K0202), Opti‐MEM medium (Gibco, 31985070), lipid peroxide sensor (LPS; MKBIO, 217075‐36‐0), NADPH assay kit with WST‐8 (Beyotime, S0179), GSH assay kit (Beyotime, S0053), bicinchoninic acid kit (Thermo Fisher Scientific, 23222), Iron ion detection kit (Leagene, TC1015), D‐luciferin, sodium salt (Meilunbio, MB1837), radioimmunoprecipitation assay (RIPA) buffer (Solarbio, R0020), Proease inhibitor cocktail (Solarbio, A8260), and NRF2 protein (MedChemExpress, HY‐P72308). .. An in vitro BBB model was constructed using six‐well transwell plates (Corning, Inc. Corning, NY, USA) using the bEnd.3 cell line.
GSH Assay:Article Title: Procyanidin B1 Promotes PSMC3-NRF2 Ubiquitination to Induce Ferroptosis in Glioblastoma.
Article Snippet: Antibodies used included anti- NRF2 (Abcam; cat, ab31163; lot, GR3248767), anti- NRF2 (Proteintech; cat, 16396- 1- AP; lot, 2354154), anti- KI- 67 (Abcam; cat, ab15580; lot, GR3362242), anti- SLC7A11 (MedChemExpress; cat, YA006; lot, 267113), anti- COX2 (Abcam; cat, ab179800; lot, GR222252), and antiTFR1 (Abcam; cat, ab214039; lot, GR3235217). .. Reagents used included erastin (MedChemExpress, HY- 15763), ferrostatin- 1 (MedChemExpress, HY- 100579), procyanidin B1 (Meilunbio, MB7155- 1, HPLC ≥95%), temozolomide (TMZ; MedChemExpress, HY- 17364), Cell counting kit- 8 (CCK- 8; MedChemExpress, HY- K0301), Lipofectamine 3000 transfection kit (Thermo, L3000001), calcein/ propidium iodide (PI) cell viability/cytotoxicity assay kit (Beyotime, C2015S), DMEM medium (Gibco, 11965092), FBS (Gibco, A5670701), penicillin and streptomycin (Solarbio, P1400), mRNA reverse transcription kit (Thermo Scientific, K16225), TRIzol reagent kit (Invitrogen, 15596018CN), Amplex Red kit (Beyotime, amplex red kit), Protein A/G Magnetic Beads (MedChemExpress, HYK0202), Opti- MEM medium (Gibco, 31985070), lipid peroxide sensor (LPS; MKBIO, 217075- 36- 0), NADPH assay kit with WST- 8 (Beyotime, S0179), GSH assay kit (Beyotime, S0053), bicinchoninic acid kit (Thermo Fisher Scientific, 23222), Iron ion detection kit (Leagene, TC1015), D- luciferin, sodium salt (Meilunbio, MB1837), radioimmunoprecipitation assay (RIPA) buffer (Solarbio, R0020), Proease inhibitor cocktail (Solarbio, A8260), and NRF2 protein (MedChemExpress, HY- P72308). .. An in vitro BBB model was constructed using six- well transwell plates (Corning, Inc. Corning, NY, USA) using the bEnd.3 cell line.
Article Title: Procyanidin B1 Promotes PSMC3‐NRF2 Ubiquitination to Induce Ferroptosis in Glioblastoma
Article Snippet: Antibodies used included anti‐NRF2 (Abcam; cat, ab31163; lot, GR3248767), anti‐NRF2 (Proteintech; cat, 16396‐1‐AP; lot, 2354154), anti‐KI‐67 (Abcam; cat, ab15580; lot, GR3362242), anti‐SLC7A11 (MedChemExpress; cat, YA006; lot, 267113), anti‐COX2 (Abcam; cat, ab179800; lot, GR222252), and anti‐TFR1 (Abcam; cat, ab214039; lot, GR3235217). .. Reagents used included erastin (MedChemExpress, HY‐15763), ferrostatin‐1 (MedChemExpress, HY‐100579), procyanidin B1 (Meilunbio, MB7155‐1, HPLC ≥95%), temozolomide (TMZ; MedChemExpress, HY‐17364), Cell counting kit‐8 (CCK‐8; MedChemExpress, HY‐K0301), Lipofectamine 3000 transfection kit (Thermo, L3000001), calcein/ propidium iodide (PI) cell viability/cytotoxicity assay kit (Beyotime, C2015S), DMEM medium (Gibco, 11965092), FBS (Gibco, A5670701), penicillin and streptomycin (Solarbio, P1400), mRNA reverse transcription kit (Thermo Scientific, K16225), TRIzol reagent kit (Invitrogen, 15596018CN), Amplex Red kit (Beyotime, amplex red kit), Protein A/G Magnetic Beads (MedChemExpress, HY‐K0202), Opti‐MEM medium (Gibco, 31985070), lipid peroxide sensor (LPS; MKBIO, 217075‐36‐0), NADPH assay kit with WST‐8 (Beyotime, S0179), GSH assay kit (Beyotime, S0053), bicinchoninic acid kit (Thermo Fisher Scientific, 23222), Iron ion detection kit (Leagene, TC1015), D‐luciferin, sodium salt (Meilunbio, MB1837), radioimmunoprecipitation assay (RIPA) buffer (Solarbio, R0020), Proease inhibitor cocktail (Solarbio, A8260), and NRF2 protein (MedChemExpress, HY‐P72308). .. An in vitro BBB model was constructed using six‐well transwell plates (Corning, Inc. Corning, NY, USA) using the bEnd.3 cell line.
Radio Immunoprecipitation:Article Title: Procyanidin B1 Promotes PSMC3-NRF2 Ubiquitination to Induce Ferroptosis in Glioblastoma.
Article Snippet: Antibodies used included anti- NRF2 (Abcam; cat, ab31163; lot, GR3248767), anti- NRF2 (Proteintech; cat, 16396- 1- AP; lot, 2354154), anti- KI- 67 (Abcam; cat, ab15580; lot, GR3362242), anti- SLC7A11 (MedChemExpress; cat, YA006; lot, 267113), anti- COX2 (Abcam; cat, ab179800; lot, GR222252), and antiTFR1 (Abcam; cat, ab214039; lot, GR3235217). .. Reagents used included erastin (MedChemExpress, HY- 15763), ferrostatin- 1 (MedChemExpress, HY- 100579), procyanidin B1 (Meilunbio, MB7155- 1, HPLC ≥95%), temozolomide (TMZ; MedChemExpress, HY- 17364), Cell counting kit- 8 (CCK- 8; MedChemExpress, HY- K0301), Lipofectamine 3000 transfection kit (Thermo, L3000001), calcein/ propidium iodide (PI) cell viability/cytotoxicity assay kit (Beyotime, C2015S), DMEM medium (Gibco, 11965092), FBS (Gibco, A5670701), penicillin and streptomycin (Solarbio, P1400), mRNA reverse transcription kit (Thermo Scientific, K16225), TRIzol reagent kit (Invitrogen, 15596018CN), Amplex Red kit (Beyotime, amplex red kit), Protein A/G Magnetic Beads (MedChemExpress, HYK0202), Opti- MEM medium (Gibco, 31985070), lipid peroxide sensor (LPS; MKBIO, 217075- 36- 0), NADPH assay kit with WST- 8 (Beyotime, S0179), GSH assay kit (Beyotime, S0053), bicinchoninic acid kit (Thermo Fisher Scientific, 23222), Iron ion detection kit (Leagene, TC1015), D- luciferin, sodium salt (Meilunbio, MB1837), radioimmunoprecipitation assay (RIPA) buffer (Solarbio, R0020), Proease inhibitor cocktail (Solarbio, A8260), and NRF2 protein (MedChemExpress, HY- P72308). .. An in vitro BBB model was constructed using six- well transwell plates (Corning, Inc. Corning, NY, USA) using the bEnd.3 cell line.
Article Title: Procyanidin B1 Promotes PSMC3‐NRF2 Ubiquitination to Induce Ferroptosis in Glioblastoma
Article Snippet: Antibodies used included anti‐NRF2 (Abcam; cat, ab31163; lot, GR3248767), anti‐NRF2 (Proteintech; cat, 16396‐1‐AP; lot, 2354154), anti‐KI‐67 (Abcam; cat, ab15580; lot, GR3362242), anti‐SLC7A11 (MedChemExpress; cat, YA006; lot, 267113), anti‐COX2 (Abcam; cat, ab179800; lot, GR222252), and anti‐TFR1 (Abcam; cat, ab214039; lot, GR3235217). .. Reagents used included erastin (MedChemExpress, HY‐15763), ferrostatin‐1 (MedChemExpress, HY‐100579), procyanidin B1 (Meilunbio, MB7155‐1, HPLC ≥95%), temozolomide (TMZ; MedChemExpress, HY‐17364), Cell counting kit‐8 (CCK‐8; MedChemExpress, HY‐K0301), Lipofectamine 3000 transfection kit (Thermo, L3000001), calcein/ propidium iodide (PI) cell viability/cytotoxicity assay kit (Beyotime, C2015S), DMEM medium (Gibco, 11965092), FBS (Gibco, A5670701), penicillin and streptomycin (Solarbio, P1400), mRNA reverse transcription kit (Thermo Scientific, K16225), TRIzol reagent kit (Invitrogen, 15596018CN), Amplex Red kit (Beyotime, amplex red kit), Protein A/G Magnetic Beads (MedChemExpress, HY‐K0202), Opti‐MEM medium (Gibco, 31985070), lipid peroxide sensor (LPS; MKBIO, 217075‐36‐0), NADPH assay kit with WST‐8 (Beyotime, S0179), GSH assay kit (Beyotime, S0053), bicinchoninic acid kit (Thermo Fisher Scientific, 23222), Iron ion detection kit (Leagene, TC1015), D‐luciferin, sodium salt (Meilunbio, MB1837), radioimmunoprecipitation assay (RIPA) buffer (Solarbio, R0020), Proease inhibitor cocktail (Solarbio, A8260), and NRF2 protein (MedChemExpress, HY‐P72308). .. An in vitro BBB model was constructed using six‐well transwell plates (Corning, Inc. Corning, NY, USA) using the bEnd.3 cell line.
Concentration Assay:Article Title: VDLIN: A Deep Learning‐Based Platform for Methylcobalamin‐Inspired Immunomodulatory Compound Screening
Article Snippet: .. Each inhibitor was dissolved in anhydrous DMSO and diluted to its respective working concentration: C29 (10 μM, S6597, Selleck) served as a TLR2 inhibitor; Procyanidin B1 (30 μM, HY‐N0795, MedChemExpress) acted as a TLR4 inhibitor; MyD88‐IN‐1 (30 μM, HY‐149992, MedChemExpress) was used to inhibit MyD88; Pepinh‐TRIF TFA (30 μM, HY‐P2565, MedChemExpress) functioned as a TRIF inhibitor; and GSK8612 (5 μM, T5540, TargetMol) inhibited TBK1/IKKε. ..
Article Title: VDLIN: A Deep Learning-Based Platform for Methylcobalamin-Inspired Immunomodulatory Compound Screening.
Article Snippet: .. Each inhibitor was dissolved in anhydrous DMSO and diluted to its respective working concentration: C29 (10 μM, S6597, Selleck) served as a TLR2 inhibitor; Procyanidin B1 (30 μM, HY-N0795, MedChemExpress) acted as a TLR4 inhibitor; MyD88-IN-1 (30 μM, HY149992, MedChemExpress) was used to inhibit MyD88; Pepinh-TRIF TFA (30 μM,HY-P2565, MedChemExpress) functioned as a TRIF inhibitor; and GSK8612 (5 μM, T5540, TargetMol) inhibited TBK1/IKKε. ..
Inhibition:Article Title: Phosphines-Nitrogen-Phosphines Chelated CoCl2 Exhibits Potent Antitumor Activity in Pancreatic Cancer
Article Snippet: .. The following inhibitors were used: C29 (10 μM, S6597, Selleck) for TLR2 inhibition, Procyanidin B1 (30 μM, HY-N0795, MedChemExpress) for TLR4 inhibition, MyD88-IN-1 (30 μM, HY-149992, MedChemExpress) for MyD88 inhibition, Pepinh-TRIF TFA (30 μM, HYP2565, MedChemExpress) for TRIF inhibition, GSK8612 (5 μM, T5540, TargetMol) for TBK1/IKKε inhibition, MNS (10 μM, HY-78263, MedChemExpress) for SYK inhibition, and Fludarabine (10 μM, HYB0069, MedChemExpress) for STAT1 inhibition. ..
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