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isorhamnetin ih  (MedChemExpress)


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    Structured Review

    MedChemExpress isorhamnetin ih
    Isorhamnetin Ih, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hy-n0776/Isorhamnetin/pm37537191-70-0-5
    Average 94 stars, based on 13 article reviews
    isorhamnetin ih - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    CCK-8 Assay:

    Article Title: Isorhamnetin from Astragalus membranaceus exerts anti-glioma effects via PI3K/Akt signaling pathway
    Article Snippet: The Cell Counting Kit 8 (CCK8) assay was obtained from Beyotime (USA).The Cell Counting Kit 8 (CCK8) assay was obtained from Beyotime (USA).. Isorhamnetin was acquired from MedChemExpress (MCE, https://www.medchemexpress.com).. The Annexin V/PI apoptosis detection kit was also obtained from Beyotime (Shanghai, China).The Annexin V/PI apoptosis detection kit was also obtained from Beyotime (Shanghai, China).

    Article Title: Network Pharmacology and Experimental Validation Reveal Aidi Injection Suppresses Prostate Cancer via EGFR Inhibition and STAT3-Mediated Apoptosis.
    Article Snippet: he following compounds: Quercetin (Selleck, S2391), Kaempferol (MedChemExpress, HY-14590), Isorhamnetin (MedChemExpress, HY-N0776), and Cantharidin (MedChemExpress, HY-N0209), sourced from Selleck and MCE. Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20℃. The CCK-8 and DAPI kits were from Solarbio Life Sciences (Beijing, China), while the IP-10 (CXCL10) Mouse ELISA K

    Article Title: Network Pharmacology and Experimental Validation Reveal Aidi Injection Suppresses Prostate Cancer via EGFR Inhibition and STAT3-Mediated Apoptosis
    Article Snippet: RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO 2 .RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO 2 .. The treatments included the following compounds: Quercetin (Selleck, S2391), Kaempferol (MedChemExpress, HY-14590), Isorhamnetin (MedChemExpress, HY-N0776), and Cantharidin (MedChemExpress, HY-N0209), sourced from Selleck and MCE.. Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.

    Article Title: Network Pharmacology and Experimental Validation Reveal Aidi Injection Suppresses Prostate Cancer via EGFR Inhibition and STAT3-Mediated Apoptosis.
    Article Snippet: RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO2.RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO2.. The treatments included the following compounds: Quercetin (Selleck, S2391), Kaempferol (MedChemExpress, HY-14590), Isorhamnetin (MedChemExpress, HY-N0776), and Cantharidin (MedChemExpress, HY-N0209), sourced from Selleck and MCE.. Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.

    Article Title: Inhibitory Effect of Phenolic Compounds on Vascular Endothelial Growth Factor-Induced Retinal Endothelial Permeability and Angiogenesis
    Article Snippet: Syringic acid, (−)-epicatechin gallate, (−)-epigallocatechin, (−)-epicatechin, vanillic acid, catechin, protocatechuic acid, sinapinic acid, resveratrol, luteolin, gallic acid, naringenin, ferulic acid, apigenin, caffeic acid, formononetin, biochanin A, galangin, p-coumaric acid, isorhamnetin, kaempferol, myricetin, daidzein, glycitein, tangeretin, hesperetin, cyanidin chloride, genistein, naringin, fisetin, quercetin, narirutin, tannic acid, eriodictyol, sorafenib, and lutein were purchased from MedChemExpress. .. M

    Article Title: Targeting senescence induced by age or chemotherapy with a polyphenol-rich natural extract improves longevity and healthspan in mice.
    Article Snippet: Individual constituents used in Fig. 5b were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.Individual constituents used in Fig. 5b were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.. Tested compounds include 3,4-dicaffeoylquinic acid (MedChem Express, cat. no. HY-N0057), kaempferol (MedChem Express, cat. no. HY-14590), isorhamnetin (MedChem Express, cat. no. HY-N0776), isoquercetin (MedChem Express, cat. no. HY-N1445), luteolin (Tocris Bioscience, cat. no. 2874), luteolin-7-O-glucuronide (MedChem Express, cat. no. HY-N1463), nobiletin (MedChem Express, cat. no. HY-N015) and rosmarinic acid (MedChem Express, cat. no. HY-N0529).

    Article Title: Targeting senescence induced by age or chemotherapy with a polyphenol-rich natural extract improves longevity and healthspan in mice
    Article Snippet: Individual constituents used in Fig. were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.Individual constituents used in Fig. were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.. Tested compounds include 3,4-dicaffeoylquinic acid (MedChem Express, cat. no. HY-N0057), kaempferol (MedChem Express, cat. no. HY-14590), isorhamnetin (MedChem Express, cat. no. HY-N0776), isoquercetin (MedChem Express, cat. no. HY-N1445), luteolin (Tocris Bioscience, cat. no. 2874), luteolin-7-O-glucuronide (MedChem Express, cat. no. HY-N1463), nobiletin (MedChem Express, cat. no. HY-N015) and rosmarinic acid (MedChem Express, cat. no. HY-N0529).

    Microscopy:

    Article Title: Isorhamnetin from Astragalus membranaceus exerts anti-glioma effects via PI3K/Akt signaling pathway
    Article Snippet: The Cell Counting Kit 8 (CCK8) assay was obtained from Beyotime (USA).The Cell Counting Kit 8 (CCK8) assay was obtained from Beyotime (USA).. Isorhamnetin was acquired from MedChemExpress (MCE, https://www.medchemexpress.com).. The Annexin V/PI apoptosis detection kit was also obtained from Beyotime (Shanghai, China).The Annexin V/PI apoptosis detection kit was also obtained from Beyotime (Shanghai, China).

    Article Title: Network Pharmacology and Experimental Validation Reveal Aidi Injection Suppresses Prostate Cancer via EGFR Inhibition and STAT3-Mediated Apoptosis.
    Article Snippet: he following compounds: Quercetin (Selleck, S2391), Kaempferol (MedChemExpress, HY-14590), Isorhamnetin (MedChemExpress, HY-N0776), and Cantharidin (MedChemExpress, HY-N0209), sourced from Selleck and MCE. Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20℃. The CCK-8 and DAPI kits were from Solarbio Life Sciences (Beijing, China), while the IP-10 (CXCL10) Mouse ELISA K

    Article Title: Network Pharmacology and Experimental Validation Reveal Aidi Injection Suppresses Prostate Cancer via EGFR Inhibition and STAT3-Mediated Apoptosis
    Article Snippet: RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO 2 .RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO 2 .. The treatments included the following compounds: Quercetin (Selleck, S2391), Kaempferol (MedChemExpress, HY-14590), Isorhamnetin (MedChemExpress, HY-N0776), and Cantharidin (MedChemExpress, HY-N0209), sourced from Selleck and MCE.. Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.

    Article Title: Network Pharmacology and Experimental Validation Reveal Aidi Injection Suppresses Prostate Cancer via EGFR Inhibition and STAT3-Mediated Apoptosis.
    Article Snippet: RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO2.RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO2.. The treatments included the following compounds: Quercetin (Selleck, S2391), Kaempferol (MedChemExpress, HY-14590), Isorhamnetin (MedChemExpress, HY-N0776), and Cantharidin (MedChemExpress, HY-N0209), sourced from Selleck and MCE.. Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.

    Article Title: Inhibitory Effect of Phenolic Compounds on Vascular Endothelial Growth Factor-Induced Retinal Endothelial Permeability and Angiogenesis
    Article Snippet: Syringic acid, (−)-epicatechin gallate, (−)-epigallocatechin, (−)-epicatechin, vanillic acid, catechin, protocatechuic acid, sinapinic acid, resveratrol, luteolin, gallic acid, naringenin, ferulic acid, apigenin, caffeic acid, formononetin, biochanin A, galangin, p-coumaric acid, isorhamnetin, kaempferol, myricetin, daidzein, glycitein, tangeretin, hesperetin, cyanidin chloride, genistein, naringin, fisetin, quercetin, narirutin, tannic acid, eriodictyol, sorafenib, and lutein were purchased from MedChemExpress. .. M

    Article Title: Targeting senescence induced by age or chemotherapy with a polyphenol-rich natural extract improves longevity and healthspan in mice.
    Article Snippet: Individual constituents used in Fig. 5b were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.Individual constituents used in Fig. 5b were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.. Tested compounds include 3,4-dicaffeoylquinic acid (MedChem Express, cat. no. HY-N0057), kaempferol (MedChem Express, cat. no. HY-14590), isorhamnetin (MedChem Express, cat. no. HY-N0776), isoquercetin (MedChem Express, cat. no. HY-N1445), luteolin (Tocris Bioscience, cat. no. 2874), luteolin-7-O-glucuronide (MedChem Express, cat. no. HY-N1463), nobiletin (MedChem Express, cat. no. HY-N015) and rosmarinic acid (MedChem Express, cat. no. HY-N0529).

    Article Title: Targeting senescence induced by age or chemotherapy with a polyphenol-rich natural extract improves longevity and healthspan in mice
    Article Snippet: Individual constituents used in Fig. were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.Individual constituents used in Fig. were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.. Tested compounds include 3,4-dicaffeoylquinic acid (MedChem Express, cat. no. HY-N0057), kaempferol (MedChem Express, cat. no. HY-14590), isorhamnetin (MedChem Express, cat. no. HY-N0776), isoquercetin (MedChem Express, cat. no. HY-N1445), luteolin (Tocris Bioscience, cat. no. 2874), luteolin-7-O-glucuronide (MedChem Express, cat. no. HY-N1463), nobiletin (MedChem Express, cat. no. HY-N015) and rosmarinic acid (MedChem Express, cat. no. HY-N0529).

    Flow Cytometry:

    Article Title: Isorhamnetin from Astragalus membranaceus exerts anti-glioma effects via PI3K/Akt signaling pathway
    Article Snippet: The Cell Counting Kit 8 (CCK8) assay was obtained from Beyotime (USA).The Cell Counting Kit 8 (CCK8) assay was obtained from Beyotime (USA).. Isorhamnetin was acquired from MedChemExpress (MCE, https://www.medchemexpress.com).. The Annexin V/PI apoptosis detection kit was also obtained from Beyotime (Shanghai, China).The Annexin V/PI apoptosis detection kit was also obtained from Beyotime (Shanghai, China).

    Article Title: Network Pharmacology and Experimental Validation Reveal Aidi Injection Suppresses Prostate Cancer via EGFR Inhibition and STAT3-Mediated Apoptosis.
    Article Snippet: he following compounds: Quercetin (Selleck, S2391), Kaempferol (MedChemExpress, HY-14590), Isorhamnetin (MedChemExpress, HY-N0776), and Cantharidin (MedChemExpress, HY-N0209), sourced from Selleck and MCE. Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20℃. The CCK-8 and DAPI kits were from Solarbio Life Sciences (Beijing, China), while the IP-10 (CXCL10) Mouse ELISA K

    Article Title: Network Pharmacology and Experimental Validation Reveal Aidi Injection Suppresses Prostate Cancer via EGFR Inhibition and STAT3-Mediated Apoptosis
    Article Snippet: RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO 2 .RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO 2 .. The treatments included the following compounds: Quercetin (Selleck, S2391), Kaempferol (MedChemExpress, HY-14590), Isorhamnetin (MedChemExpress, HY-N0776), and Cantharidin (MedChemExpress, HY-N0209), sourced from Selleck and MCE.. Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.

    Article Title: Network Pharmacology and Experimental Validation Reveal Aidi Injection Suppresses Prostate Cancer via EGFR Inhibition and STAT3-Mediated Apoptosis.
    Article Snippet: RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO2.RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO2.. The treatments included the following compounds: Quercetin (Selleck, S2391), Kaempferol (MedChemExpress, HY-14590), Isorhamnetin (MedChemExpress, HY-N0776), and Cantharidin (MedChemExpress, HY-N0209), sourced from Selleck and MCE.. Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.

    Article Title: Inhibitory Effect of Phenolic Compounds on Vascular Endothelial Growth Factor-Induced Retinal Endothelial Permeability and Angiogenesis
    Article Snippet: Syringic acid, (−)-epicatechin gallate, (−)-epigallocatechin, (−)-epicatechin, vanillic acid, catechin, protocatechuic acid, sinapinic acid, resveratrol, luteolin, gallic acid, naringenin, ferulic acid, apigenin, caffeic acid, formononetin, biochanin A, galangin, p-coumaric acid, isorhamnetin, kaempferol, myricetin, daidzein, glycitein, tangeretin, hesperetin, cyanidin chloride, genistein, naringin, fisetin, quercetin, narirutin, tannic acid, eriodictyol, sorafenib, and lutein were purchased from MedChemExpress. .. M

    Article Title: Targeting senescence induced by age or chemotherapy with a polyphenol-rich natural extract improves longevity and healthspan in mice.
    Article Snippet: Individual constituents used in Fig. 5b were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.Individual constituents used in Fig. 5b were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.. Tested compounds include 3,4-dicaffeoylquinic acid (MedChem Express, cat. no. HY-N0057), kaempferol (MedChem Express, cat. no. HY-14590), isorhamnetin (MedChem Express, cat. no. HY-N0776), isoquercetin (MedChem Express, cat. no. HY-N1445), luteolin (Tocris Bioscience, cat. no. 2874), luteolin-7-O-glucuronide (MedChem Express, cat. no. HY-N1463), nobiletin (MedChem Express, cat. no. HY-N015) and rosmarinic acid (MedChem Express, cat. no. HY-N0529).

    Article Title: Targeting senescence induced by age or chemotherapy with a polyphenol-rich natural extract improves longevity and healthspan in mice
    Article Snippet: Individual constituents used in Fig. were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.Individual constituents used in Fig. were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.. Tested compounds include 3,4-dicaffeoylquinic acid (MedChem Express, cat. no. HY-N0057), kaempferol (MedChem Express, cat. no. HY-14590), isorhamnetin (MedChem Express, cat. no. HY-N0776), isoquercetin (MedChem Express, cat. no. HY-N1445), luteolin (Tocris Bioscience, cat. no. 2874), luteolin-7-O-glucuronide (MedChem Express, cat. no. HY-N1463), nobiletin (MedChem Express, cat. no. HY-N015) and rosmarinic acid (MedChem Express, cat. no. HY-N0529).

    Migration:

    Article Title: Isorhamnetin from Astragalus membranaceus exerts anti-glioma effects via PI3K/Akt signaling pathway
    Article Snippet: The Cell Counting Kit 8 (CCK8) assay was obtained from Beyotime (USA).The Cell Counting Kit 8 (CCK8) assay was obtained from Beyotime (USA).. Isorhamnetin was acquired from MedChemExpress (MCE, https://www.medchemexpress.com).. The Annexin V/PI apoptosis detection kit was also obtained from Beyotime (Shanghai, China).The Annexin V/PI apoptosis detection kit was also obtained from Beyotime (Shanghai, China).

    Article Title: Network Pharmacology and Experimental Validation Reveal Aidi Injection Suppresses Prostate Cancer via EGFR Inhibition and STAT3-Mediated Apoptosis.
    Article Snippet: he following compounds: Quercetin (Selleck, S2391), Kaempferol (MedChemExpress, HY-14590), Isorhamnetin (MedChemExpress, HY-N0776), and Cantharidin (MedChemExpress, HY-N0209), sourced from Selleck and MCE. Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20℃. The CCK-8 and DAPI kits were from Solarbio Life Sciences (Beijing, China), while the IP-10 (CXCL10) Mouse ELISA K

    Article Title: Network Pharmacology and Experimental Validation Reveal Aidi Injection Suppresses Prostate Cancer via EGFR Inhibition and STAT3-Mediated Apoptosis
    Article Snippet: RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO 2 .RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO 2 .. The treatments included the following compounds: Quercetin (Selleck, S2391), Kaempferol (MedChemExpress, HY-14590), Isorhamnetin (MedChemExpress, HY-N0776), and Cantharidin (MedChemExpress, HY-N0209), sourced from Selleck and MCE.. Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.

    Article Title: Network Pharmacology and Experimental Validation Reveal Aidi Injection Suppresses Prostate Cancer via EGFR Inhibition and STAT3-Mediated Apoptosis.
    Article Snippet: RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO2.RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO2.. The treatments included the following compounds: Quercetin (Selleck, S2391), Kaempferol (MedChemExpress, HY-14590), Isorhamnetin (MedChemExpress, HY-N0776), and Cantharidin (MedChemExpress, HY-N0209), sourced from Selleck and MCE.. Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.

    Article Title: Inhibitory Effect of Phenolic Compounds on Vascular Endothelial Growth Factor-Induced Retinal Endothelial Permeability and Angiogenesis
    Article Snippet: Syringic acid, (−)-epicatechin gallate, (−)-epigallocatechin, (−)-epicatechin, vanillic acid, catechin, protocatechuic acid, sinapinic acid, resveratrol, luteolin, gallic acid, naringenin, ferulic acid, apigenin, caffeic acid, formononetin, biochanin A, galangin, p-coumaric acid, isorhamnetin, kaempferol, myricetin, daidzein, glycitein, tangeretin, hesperetin, cyanidin chloride, genistein, naringin, fisetin, quercetin, narirutin, tannic acid, eriodictyol, sorafenib, and lutein were purchased from MedChemExpress. .. M

    Article Title: Targeting senescence induced by age or chemotherapy with a polyphenol-rich natural extract improves longevity and healthspan in mice.
    Article Snippet: Individual constituents used in Fig. 5b were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.Individual constituents used in Fig. 5b were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.. Tested compounds include 3,4-dicaffeoylquinic acid (MedChem Express, cat. no. HY-N0057), kaempferol (MedChem Express, cat. no. HY-14590), isorhamnetin (MedChem Express, cat. no. HY-N0776), isoquercetin (MedChem Express, cat. no. HY-N1445), luteolin (Tocris Bioscience, cat. no. 2874), luteolin-7-O-glucuronide (MedChem Express, cat. no. HY-N1463), nobiletin (MedChem Express, cat. no. HY-N015) and rosmarinic acid (MedChem Express, cat. no. HY-N0529).

    Article Title: Targeting senescence induced by age or chemotherapy with a polyphenol-rich natural extract improves longevity and healthspan in mice
    Article Snippet: Individual constituents used in Fig. were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.Individual constituents used in Fig. were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.. Tested compounds include 3,4-dicaffeoylquinic acid (MedChem Express, cat. no. HY-N0057), kaempferol (MedChem Express, cat. no. HY-14590), isorhamnetin (MedChem Express, cat. no. HY-N0776), isoquercetin (MedChem Express, cat. no. HY-N1445), luteolin (Tocris Bioscience, cat. no. 2874), luteolin-7-O-glucuronide (MedChem Express, cat. no. HY-N1463), nobiletin (MedChem Express, cat. no. HY-N015) and rosmarinic acid (MedChem Express, cat. no. HY-N0529).

    Inhibition:

    Article Title: Isorhamnetin from Astragalus membranaceus exerts anti-glioma effects via PI3K/Akt signaling pathway
    Article Snippet: The Cell Counting Kit 8 (CCK8) assay was obtained from Beyotime (USA).The Cell Counting Kit 8 (CCK8) assay was obtained from Beyotime (USA).. Isorhamnetin was acquired from MedChemExpress (MCE, https://www.medchemexpress.com).. The Annexin V/PI apoptosis detection kit was also obtained from Beyotime (Shanghai, China).The Annexin V/PI apoptosis detection kit was also obtained from Beyotime (Shanghai, China).

    Article Title: Network Pharmacology and Experimental Validation Reveal Aidi Injection Suppresses Prostate Cancer via EGFR Inhibition and STAT3-Mediated Apoptosis.
    Article Snippet: he following compounds: Quercetin (Selleck, S2391), Kaempferol (MedChemExpress, HY-14590), Isorhamnetin (MedChemExpress, HY-N0776), and Cantharidin (MedChemExpress, HY-N0209), sourced from Selleck and MCE. Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20℃. The CCK-8 and DAPI kits were from Solarbio Life Sciences (Beijing, China), while the IP-10 (CXCL10) Mouse ELISA K

    Article Title: Network Pharmacology and Experimental Validation Reveal Aidi Injection Suppresses Prostate Cancer via EGFR Inhibition and STAT3-Mediated Apoptosis
    Article Snippet: RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO 2 .RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO 2 .. The treatments included the following compounds: Quercetin (Selleck, S2391), Kaempferol (MedChemExpress, HY-14590), Isorhamnetin (MedChemExpress, HY-N0776), and Cantharidin (MedChemExpress, HY-N0209), sourced from Selleck and MCE.. Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.

    Article Title: Network Pharmacology and Experimental Validation Reveal Aidi Injection Suppresses Prostate Cancer via EGFR Inhibition and STAT3-Mediated Apoptosis.
    Article Snippet: RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO2.RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO2.. The treatments included the following compounds: Quercetin (Selleck, S2391), Kaempferol (MedChemExpress, HY-14590), Isorhamnetin (MedChemExpress, HY-N0776), and Cantharidin (MedChemExpress, HY-N0209), sourced from Selleck and MCE.. Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.

    Article Title: Inhibitory Effect of Phenolic Compounds on Vascular Endothelial Growth Factor-Induced Retinal Endothelial Permeability and Angiogenesis
    Article Snippet: Syringic acid, (−)-epicatechin gallate, (−)-epigallocatechin, (−)-epicatechin, vanillic acid, catechin, protocatechuic acid, sinapinic acid, resveratrol, luteolin, gallic acid, naringenin, ferulic acid, apigenin, caffeic acid, formononetin, biochanin A, galangin, p-coumaric acid, isorhamnetin, kaempferol, myricetin, daidzein, glycitein, tangeretin, hesperetin, cyanidin chloride, genistein, naringin, fisetin, quercetin, narirutin, tannic acid, eriodictyol, sorafenib, and lutein were purchased from MedChemExpress. .. M

    Article Title: Targeting senescence induced by age or chemotherapy with a polyphenol-rich natural extract improves longevity and healthspan in mice.
    Article Snippet: Individual constituents used in Fig. 5b were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.Individual constituents used in Fig. 5b were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.. Tested compounds include 3,4-dicaffeoylquinic acid (MedChem Express, cat. no. HY-N0057), kaempferol (MedChem Express, cat. no. HY-14590), isorhamnetin (MedChem Express, cat. no. HY-N0776), isoquercetin (MedChem Express, cat. no. HY-N1445), luteolin (Tocris Bioscience, cat. no. 2874), luteolin-7-O-glucuronide (MedChem Express, cat. no. HY-N1463), nobiletin (MedChem Express, cat. no. HY-N015) and rosmarinic acid (MedChem Express, cat. no. HY-N0529).

    Article Title: Targeting senescence induced by age or chemotherapy with a polyphenol-rich natural extract improves longevity and healthspan in mice
    Article Snippet: Individual constituents used in Fig. were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.Individual constituents used in Fig. were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.. Tested compounds include 3,4-dicaffeoylquinic acid (MedChem Express, cat. no. HY-N0057), kaempferol (MedChem Express, cat. no. HY-14590), isorhamnetin (MedChem Express, cat. no. HY-N0776), isoquercetin (MedChem Express, cat. no. HY-N1445), luteolin (Tocris Bioscience, cat. no. 2874), luteolin-7-O-glucuronide (MedChem Express, cat. no. HY-N1463), nobiletin (MedChem Express, cat. no. HY-N015) and rosmarinic acid (MedChem Express, cat. no. HY-N0529).

    Expressing:

    Article Title: Isorhamnetin from Astragalus membranaceus exerts anti-glioma effects via PI3K/Akt signaling pathway
    Article Snippet: The Cell Counting Kit 8 (CCK8) assay was obtained from Beyotime (USA).The Cell Counting Kit 8 (CCK8) assay was obtained from Beyotime (USA).. Isorhamnetin was acquired from MedChemExpress (MCE, https://www.medchemexpress.com).. The Annexin V/PI apoptosis detection kit was also obtained from Beyotime (Shanghai, China).The Annexin V/PI apoptosis detection kit was also obtained from Beyotime (Shanghai, China).

    Article Title: Network Pharmacology and Experimental Validation Reveal Aidi Injection Suppresses Prostate Cancer via EGFR Inhibition and STAT3-Mediated Apoptosis.
    Article Snippet: he following compounds: Quercetin (Selleck, S2391), Kaempferol (MedChemExpress, HY-14590), Isorhamnetin (MedChemExpress, HY-N0776), and Cantharidin (MedChemExpress, HY-N0209), sourced from Selleck and MCE. Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20℃. The CCK-8 and DAPI kits were from Solarbio Life Sciences (Beijing, China), while the IP-10 (CXCL10) Mouse ELISA K

    Article Title: Network Pharmacology and Experimental Validation Reveal Aidi Injection Suppresses Prostate Cancer via EGFR Inhibition and STAT3-Mediated Apoptosis
    Article Snippet: RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO 2 .RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO 2 .. The treatments included the following compounds: Quercetin (Selleck, S2391), Kaempferol (MedChemExpress, HY-14590), Isorhamnetin (MedChemExpress, HY-N0776), and Cantharidin (MedChemExpress, HY-N0209), sourced from Selleck and MCE.. Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.

    Article Title: Network Pharmacology and Experimental Validation Reveal Aidi Injection Suppresses Prostate Cancer via EGFR Inhibition and STAT3-Mediated Apoptosis.
    Article Snippet: RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO2.RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO2.. The treatments included the following compounds: Quercetin (Selleck, S2391), Kaempferol (MedChemExpress, HY-14590), Isorhamnetin (MedChemExpress, HY-N0776), and Cantharidin (MedChemExpress, HY-N0209), sourced from Selleck and MCE.. Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.

    Article Title: Inhibitory Effect of Phenolic Compounds on Vascular Endothelial Growth Factor-Induced Retinal Endothelial Permeability and Angiogenesis
    Article Snippet: Syringic acid, (−)-epicatechin gallate, (−)-epigallocatechin, (−)-epicatechin, vanillic acid, catechin, protocatechuic acid, sinapinic acid, resveratrol, luteolin, gallic acid, naringenin, ferulic acid, apigenin, caffeic acid, formononetin, biochanin A, galangin, p-coumaric acid, isorhamnetin, kaempferol, myricetin, daidzein, glycitein, tangeretin, hesperetin, cyanidin chloride, genistein, naringin, fisetin, quercetin, narirutin, tannic acid, eriodictyol, sorafenib, and lutein were purchased from MedChemExpress. .. M

    Article Title: Targeting senescence induced by age or chemotherapy with a polyphenol-rich natural extract improves longevity and healthspan in mice.
    Article Snippet: Individual constituents used in Fig. 5b were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.Individual constituents used in Fig. 5b were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.. Tested compounds include 3,4-dicaffeoylquinic acid (MedChem Express, cat. no. HY-N0057), kaempferol (MedChem Express, cat. no. HY-14590), isorhamnetin (MedChem Express, cat. no. HY-N0776), isoquercetin (MedChem Express, cat. no. HY-N1445), luteolin (Tocris Bioscience, cat. no. 2874), luteolin-7-O-glucuronide (MedChem Express, cat. no. HY-N1463), nobiletin (MedChem Express, cat. no. HY-N015) and rosmarinic acid (MedChem Express, cat. no. HY-N0529).

    Article Title: Targeting senescence induced by age or chemotherapy with a polyphenol-rich natural extract improves longevity and healthspan in mice
    Article Snippet: Individual constituents used in Fig. were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.Individual constituents used in Fig. were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.. Tested compounds include 3,4-dicaffeoylquinic acid (MedChem Express, cat. no. HY-N0057), kaempferol (MedChem Express, cat. no. HY-14590), isorhamnetin (MedChem Express, cat. no. HY-N0776), isoquercetin (MedChem Express, cat. no. HY-N1445), luteolin (Tocris Bioscience, cat. no. 2874), luteolin-7-O-glucuronide (MedChem Express, cat. no. HY-N1463), nobiletin (MedChem Express, cat. no. HY-N015) and rosmarinic acid (MedChem Express, cat. no. HY-N0529).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Isorhamnetin from Astragalus membranaceus exerts anti-glioma effects via PI3K/Akt signaling pathway
    Article Snippet: The Cell Counting Kit 8 (CCK8) assay was obtained from Beyotime (USA).The Cell Counting Kit 8 (CCK8) assay was obtained from Beyotime (USA).. Isorhamnetin was acquired from MedChemExpress (MCE, https://www.medchemexpress.com).. The Annexin V/PI apoptosis detection kit was also obtained from Beyotime (Shanghai, China).The Annexin V/PI apoptosis detection kit was also obtained from Beyotime (Shanghai, China).

    Article Title: Network Pharmacology and Experimental Validation Reveal Aidi Injection Suppresses Prostate Cancer via EGFR Inhibition and STAT3-Mediated Apoptosis.
    Article Snippet: he following compounds: Quercetin (Selleck, S2391), Kaempferol (MedChemExpress, HY-14590), Isorhamnetin (MedChemExpress, HY-N0776), and Cantharidin (MedChemExpress, HY-N0209), sourced from Selleck and MCE. Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20℃. The CCK-8 and DAPI kits were from Solarbio Life Sciences (Beijing, China), while the IP-10 (CXCL10) Mouse ELISA K

    Article Title: Network Pharmacology and Experimental Validation Reveal Aidi Injection Suppresses Prostate Cancer via EGFR Inhibition and STAT3-Mediated Apoptosis
    Article Snippet: RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO 2 .RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO 2 .. The treatments included the following compounds: Quercetin (Selleck, S2391), Kaempferol (MedChemExpress, HY-14590), Isorhamnetin (MedChemExpress, HY-N0776), and Cantharidin (MedChemExpress, HY-N0209), sourced from Selleck and MCE.. Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.

    Article Title: Network Pharmacology and Experimental Validation Reveal Aidi Injection Suppresses Prostate Cancer via EGFR Inhibition and STAT3-Mediated Apoptosis.
    Article Snippet: RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO2.RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO2.. The treatments included the following compounds: Quercetin (Selleck, S2391), Kaempferol (MedChemExpress, HY-14590), Isorhamnetin (MedChemExpress, HY-N0776), and Cantharidin (MedChemExpress, HY-N0209), sourced from Selleck and MCE.. Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.

    Article Title: Inhibitory Effect of Phenolic Compounds on Vascular Endothelial Growth Factor-Induced Retinal Endothelial Permeability and Angiogenesis
    Article Snippet: Syringic acid, (−)-epicatechin gallate, (−)-epigallocatechin, (−)-epicatechin, vanillic acid, catechin, protocatechuic acid, sinapinic acid, resveratrol, luteolin, gallic acid, naringenin, ferulic acid, apigenin, caffeic acid, formononetin, biochanin A, galangin, p-coumaric acid, isorhamnetin, kaempferol, myricetin, daidzein, glycitein, tangeretin, hesperetin, cyanidin chloride, genistein, naringin, fisetin, quercetin, narirutin, tannic acid, eriodictyol, sorafenib, and lutein were purchased from MedChemExpress. .. M

    Article Title: Targeting senescence induced by age or chemotherapy with a polyphenol-rich natural extract improves longevity and healthspan in mice.
    Article Snippet: Individual constituents used in Fig. 5b were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.Individual constituents used in Fig. 5b were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.. Tested compounds include 3,4-dicaffeoylquinic acid (MedChem Express, cat. no. HY-N0057), kaempferol (MedChem Express, cat. no. HY-14590), isorhamnetin (MedChem Express, cat. no. HY-N0776), isoquercetin (MedChem Express, cat. no. HY-N1445), luteolin (Tocris Bioscience, cat. no. 2874), luteolin-7-O-glucuronide (MedChem Express, cat. no. HY-N1463), nobiletin (MedChem Express, cat. no. HY-N015) and rosmarinic acid (MedChem Express, cat. no. HY-N0529).

    Article Title: Targeting senescence induced by age or chemotherapy with a polyphenol-rich natural extract improves longevity and healthspan in mice
    Article Snippet: Individual constituents used in Fig. were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.Individual constituents used in Fig. were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.. Tested compounds include 3,4-dicaffeoylquinic acid (MedChem Express, cat. no. HY-N0057), kaempferol (MedChem Express, cat. no. HY-14590), isorhamnetin (MedChem Express, cat. no. HY-N0776), isoquercetin (MedChem Express, cat. no. HY-N1445), luteolin (Tocris Bioscience, cat. no. 2874), luteolin-7-O-glucuronide (MedChem Express, cat. no. HY-N1463), nobiletin (MedChem Express, cat. no. HY-N015) and rosmarinic acid (MedChem Express, cat. no. HY-N0529).

    Injection:

    Article Title: Isorhamnetin from Astragalus membranaceus exerts anti-glioma effects via PI3K/Akt signaling pathway
    Article Snippet: The Cell Counting Kit 8 (CCK8) assay was obtained from Beyotime (USA).The Cell Counting Kit 8 (CCK8) assay was obtained from Beyotime (USA).. Isorhamnetin was acquired from MedChemExpress (MCE, https://www.medchemexpress.com).. The Annexin V/PI apoptosis detection kit was also obtained from Beyotime (Shanghai, China).The Annexin V/PI apoptosis detection kit was also obtained from Beyotime (Shanghai, China).

    Article Title: Network Pharmacology and Experimental Validation Reveal Aidi Injection Suppresses Prostate Cancer via EGFR Inhibition and STAT3-Mediated Apoptosis.
    Article Snippet: he following compounds: Quercetin (Selleck, S2391), Kaempferol (MedChemExpress, HY-14590), Isorhamnetin (MedChemExpress, HY-N0776), and Cantharidin (MedChemExpress, HY-N0209), sourced from Selleck and MCE. Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20℃. The CCK-8 and DAPI kits were from Solarbio Life Sciences (Beijing, China), while the IP-10 (CXCL10) Mouse ELISA K

    Article Title: Network Pharmacology and Experimental Validation Reveal Aidi Injection Suppresses Prostate Cancer via EGFR Inhibition and STAT3-Mediated Apoptosis
    Article Snippet: RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO 2 .RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO 2 .. The treatments included the following compounds: Quercetin (Selleck, S2391), Kaempferol (MedChemExpress, HY-14590), Isorhamnetin (MedChemExpress, HY-N0776), and Cantharidin (MedChemExpress, HY-N0209), sourced from Selleck and MCE.. Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.

    Article Title: Network Pharmacology and Experimental Validation Reveal Aidi Injection Suppresses Prostate Cancer via EGFR Inhibition and STAT3-Mediated Apoptosis.
    Article Snippet: RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO2.RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO2.. The treatments included the following compounds: Quercetin (Selleck, S2391), Kaempferol (MedChemExpress, HY-14590), Isorhamnetin (MedChemExpress, HY-N0776), and Cantharidin (MedChemExpress, HY-N0209), sourced from Selleck and MCE.. Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.

    Article Title: Inhibitory Effect of Phenolic Compounds on Vascular Endothelial Growth Factor-Induced Retinal Endothelial Permeability and Angiogenesis
    Article Snippet: Syringic acid, (−)-epicatechin gallate, (−)-epigallocatechin, (−)-epicatechin, vanillic acid, catechin, protocatechuic acid, sinapinic acid, resveratrol, luteolin, gallic acid, naringenin, ferulic acid, apigenin, caffeic acid, formononetin, biochanin A, galangin, p-coumaric acid, isorhamnetin, kaempferol, myricetin, daidzein, glycitein, tangeretin, hesperetin, cyanidin chloride, genistein, naringin, fisetin, quercetin, narirutin, tannic acid, eriodictyol, sorafenib, and lutein were purchased from MedChemExpress. .. M

    Article Title: Targeting senescence induced by age or chemotherapy with a polyphenol-rich natural extract improves longevity and healthspan in mice.
    Article Snippet: Individual constituents used in Fig. 5b were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.Individual constituents used in Fig. 5b were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.. Tested compounds include 3,4-dicaffeoylquinic acid (MedChem Express, cat. no. HY-N0057), kaempferol (MedChem Express, cat. no. HY-14590), isorhamnetin (MedChem Express, cat. no. HY-N0776), isoquercetin (MedChem Express, cat. no. HY-N1445), luteolin (Tocris Bioscience, cat. no. 2874), luteolin-7-O-glucuronide (MedChem Express, cat. no. HY-N1463), nobiletin (MedChem Express, cat. no. HY-N015) and rosmarinic acid (MedChem Express, cat. no. HY-N0529).

    Article Title: Targeting senescence induced by age or chemotherapy with a polyphenol-rich natural extract improves longevity and healthspan in mice
    Article Snippet: Individual constituents used in Fig. were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.Individual constituents used in Fig. were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.. Tested compounds include 3,4-dicaffeoylquinic acid (MedChem Express, cat. no. HY-N0057), kaempferol (MedChem Express, cat. no. HY-14590), isorhamnetin (MedChem Express, cat. no. HY-N0776), isoquercetin (MedChem Express, cat. no. HY-N1445), luteolin (Tocris Bioscience, cat. no. 2874), luteolin-7-O-glucuronide (MedChem Express, cat. no. HY-N1463), nobiletin (MedChem Express, cat. no. HY-N015) and rosmarinic acid (MedChem Express, cat. no. HY-N0529).

    Western Blot:

    Article Title: Isorhamnetin from Astragalus membranaceus exerts anti-glioma effects via PI3K/Akt signaling pathway
    Article Snippet: The Cell Counting Kit 8 (CCK8) assay was obtained from Beyotime (USA).The Cell Counting Kit 8 (CCK8) assay was obtained from Beyotime (USA).. Isorhamnetin was acquired from MedChemExpress (MCE, https://www.medchemexpress.com).. The Annexin V/PI apoptosis detection kit was also obtained from Beyotime (Shanghai, China).The Annexin V/PI apoptosis detection kit was also obtained from Beyotime (Shanghai, China).

    Article Title: Network Pharmacology and Experimental Validation Reveal Aidi Injection Suppresses Prostate Cancer via EGFR Inhibition and STAT3-Mediated Apoptosis.
    Article Snippet: he following compounds: Quercetin (Selleck, S2391), Kaempferol (MedChemExpress, HY-14590), Isorhamnetin (MedChemExpress, HY-N0776), and Cantharidin (MedChemExpress, HY-N0209), sourced from Selleck and MCE. Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20℃. The CCK-8 and DAPI kits were from Solarbio Life Sciences (Beijing, China), while the IP-10 (CXCL10) Mouse ELISA K

    Article Title: Network Pharmacology and Experimental Validation Reveal Aidi Injection Suppresses Prostate Cancer via EGFR Inhibition and STAT3-Mediated Apoptosis
    Article Snippet: RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO 2 .RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO 2 .. The treatments included the following compounds: Quercetin (Selleck, S2391), Kaempferol (MedChemExpress, HY-14590), Isorhamnetin (MedChemExpress, HY-N0776), and Cantharidin (MedChemExpress, HY-N0209), sourced from Selleck and MCE.. Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.

    Article Title: Network Pharmacology and Experimental Validation Reveal Aidi Injection Suppresses Prostate Cancer via EGFR Inhibition and STAT3-Mediated Apoptosis.
    Article Snippet: RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO2.RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO2.. The treatments included the following compounds: Quercetin (Selleck, S2391), Kaempferol (MedChemExpress, HY-14590), Isorhamnetin (MedChemExpress, HY-N0776), and Cantharidin (MedChemExpress, HY-N0209), sourced from Selleck and MCE.. Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.

    Article Title: Inhibitory Effect of Phenolic Compounds on Vascular Endothelial Growth Factor-Induced Retinal Endothelial Permeability and Angiogenesis
    Article Snippet: Syringic acid, (−)-epicatechin gallate, (−)-epigallocatechin, (−)-epicatechin, vanillic acid, catechin, protocatechuic acid, sinapinic acid, resveratrol, luteolin, gallic acid, naringenin, ferulic acid, apigenin, caffeic acid, formononetin, biochanin A, galangin, p-coumaric acid, isorhamnetin, kaempferol, myricetin, daidzein, glycitein, tangeretin, hesperetin, cyanidin chloride, genistein, naringin, fisetin, quercetin, narirutin, tannic acid, eriodictyol, sorafenib, and lutein were purchased from MedChemExpress. .. M

    Article Title: Targeting senescence induced by age or chemotherapy with a polyphenol-rich natural extract improves longevity and healthspan in mice.
    Article Snippet: Individual constituents used in Fig. 5b were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.Individual constituents used in Fig. 5b were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.. Tested compounds include 3,4-dicaffeoylquinic acid (MedChem Express, cat. no. HY-N0057), kaempferol (MedChem Express, cat. no. HY-14590), isorhamnetin (MedChem Express, cat. no. HY-N0776), isoquercetin (MedChem Express, cat. no. HY-N1445), luteolin (Tocris Bioscience, cat. no. 2874), luteolin-7-O-glucuronide (MedChem Express, cat. no. HY-N1463), nobiletin (MedChem Express, cat. no. HY-N015) and rosmarinic acid (MedChem Express, cat. no. HY-N0529).

    Article Title: Targeting senescence induced by age or chemotherapy with a polyphenol-rich natural extract improves longevity and healthspan in mice
    Article Snippet: Individual constituents used in Fig. were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.Individual constituents used in Fig. were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.. Tested compounds include 3,4-dicaffeoylquinic acid (MedChem Express, cat. no. HY-N0057), kaempferol (MedChem Express, cat. no. HY-14590), isorhamnetin (MedChem Express, cat. no. HY-N0776), isoquercetin (MedChem Express, cat. no. HY-N1445), luteolin (Tocris Bioscience, cat. no. 2874), luteolin-7-O-glucuronide (MedChem Express, cat. no. HY-N1463), nobiletin (MedChem Express, cat. no. HY-N015) and rosmarinic acid (MedChem Express, cat. no. HY-N0529).

    Activation Assay:

    Article Title: Isorhamnetin from Astragalus membranaceus exerts anti-glioma effects via PI3K/Akt signaling pathway
    Article Snippet: The Cell Counting Kit 8 (CCK8) assay was obtained from Beyotime (USA).The Cell Counting Kit 8 (CCK8) assay was obtained from Beyotime (USA).. Isorhamnetin was acquired from MedChemExpress (MCE, https://www.medchemexpress.com).. The Annexin V/PI apoptosis detection kit was also obtained from Beyotime (Shanghai, China).The Annexin V/PI apoptosis detection kit was also obtained from Beyotime (Shanghai, China).

    Article Title: Network Pharmacology and Experimental Validation Reveal Aidi Injection Suppresses Prostate Cancer via EGFR Inhibition and STAT3-Mediated Apoptosis.
    Article Snippet: he following compounds: Quercetin (Selleck, S2391), Kaempferol (MedChemExpress, HY-14590), Isorhamnetin (MedChemExpress, HY-N0776), and Cantharidin (MedChemExpress, HY-N0209), sourced from Selleck and MCE. Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20℃. The CCK-8 and DAPI kits were from Solarbio Life Sciences (Beijing, China), while the IP-10 (CXCL10) Mouse ELISA K

    Article Title: Network Pharmacology and Experimental Validation Reveal Aidi Injection Suppresses Prostate Cancer via EGFR Inhibition and STAT3-Mediated Apoptosis
    Article Snippet: RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO 2 .RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO 2 .. The treatments included the following compounds: Quercetin (Selleck, S2391), Kaempferol (MedChemExpress, HY-14590), Isorhamnetin (MedChemExpress, HY-N0776), and Cantharidin (MedChemExpress, HY-N0209), sourced from Selleck and MCE.. Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.

    Article Title: Network Pharmacology and Experimental Validation Reveal Aidi Injection Suppresses Prostate Cancer via EGFR Inhibition and STAT3-Mediated Apoptosis.
    Article Snippet: RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO2.RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO2.. The treatments included the following compounds: Quercetin (Selleck, S2391), Kaempferol (MedChemExpress, HY-14590), Isorhamnetin (MedChemExpress, HY-N0776), and Cantharidin (MedChemExpress, HY-N0209), sourced from Selleck and MCE.. Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.

    Article Title: Inhibitory Effect of Phenolic Compounds on Vascular Endothelial Growth Factor-Induced Retinal Endothelial Permeability and Angiogenesis
    Article Snippet: Syringic acid, (−)-epicatechin gallate, (−)-epigallocatechin, (−)-epicatechin, vanillic acid, catechin, protocatechuic acid, sinapinic acid, resveratrol, luteolin, gallic acid, naringenin, ferulic acid, apigenin, caffeic acid, formononetin, biochanin A, galangin, p-coumaric acid, isorhamnetin, kaempferol, myricetin, daidzein, glycitein, tangeretin, hesperetin, cyanidin chloride, genistein, naringin, fisetin, quercetin, narirutin, tannic acid, eriodictyol, sorafenib, and lutein were purchased from MedChemExpress. .. M

    Article Title: Targeting senescence induced by age or chemotherapy with a polyphenol-rich natural extract improves longevity and healthspan in mice.
    Article Snippet: Individual constituents used in Fig. 5b were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.Individual constituents used in Fig. 5b were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.. Tested compounds include 3,4-dicaffeoylquinic acid (MedChem Express, cat. no. HY-N0057), kaempferol (MedChem Express, cat. no. HY-14590), isorhamnetin (MedChem Express, cat. no. HY-N0776), isoquercetin (MedChem Express, cat. no. HY-N1445), luteolin (Tocris Bioscience, cat. no. 2874), luteolin-7-O-glucuronide (MedChem Express, cat. no. HY-N1463), nobiletin (MedChem Express, cat. no. HY-N015) and rosmarinic acid (MedChem Express, cat. no. HY-N0529).

    Article Title: Targeting senescence induced by age or chemotherapy with a polyphenol-rich natural extract improves longevity and healthspan in mice
    Article Snippet: Individual constituents used in Fig. were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.Individual constituents used in Fig. were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.. Tested compounds include 3,4-dicaffeoylquinic acid (MedChem Express, cat. no. HY-N0057), kaempferol (MedChem Express, cat. no. HY-14590), isorhamnetin (MedChem Express, cat. no. HY-N0776), isoquercetin (MedChem Express, cat. no. HY-N1445), luteolin (Tocris Bioscience, cat. no. 2874), luteolin-7-O-glucuronide (MedChem Express, cat. no. HY-N1463), nobiletin (MedChem Express, cat. no. HY-N015) and rosmarinic acid (MedChem Express, cat. no. HY-N0529).

    Binding Assay:

    Article Title: Isorhamnetin from Astragalus membranaceus exerts anti-glioma effects via PI3K/Akt signaling pathway
    Article Snippet: The Cell Counting Kit 8 (CCK8) assay was obtained from Beyotime (USA).The Cell Counting Kit 8 (CCK8) assay was obtained from Beyotime (USA).. Isorhamnetin was acquired from MedChemExpress (MCE, https://www.medchemexpress.com).. The Annexin V/PI apoptosis detection kit was also obtained from Beyotime (Shanghai, China).The Annexin V/PI apoptosis detection kit was also obtained from Beyotime (Shanghai, China).

    Article Title: Network Pharmacology and Experimental Validation Reveal Aidi Injection Suppresses Prostate Cancer via EGFR Inhibition and STAT3-Mediated Apoptosis.
    Article Snippet: he following compounds: Quercetin (Selleck, S2391), Kaempferol (MedChemExpress, HY-14590), Isorhamnetin (MedChemExpress, HY-N0776), and Cantharidin (MedChemExpress, HY-N0209), sourced from Selleck and MCE. Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20℃. The CCK-8 and DAPI kits were from Solarbio Life Sciences (Beijing, China), while the IP-10 (CXCL10) Mouse ELISA K

    Article Title: Network Pharmacology and Experimental Validation Reveal Aidi Injection Suppresses Prostate Cancer via EGFR Inhibition and STAT3-Mediated Apoptosis
    Article Snippet: RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO 2 .RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO 2 .. The treatments included the following compounds: Quercetin (Selleck, S2391), Kaempferol (MedChemExpress, HY-14590), Isorhamnetin (MedChemExpress, HY-N0776), and Cantharidin (MedChemExpress, HY-N0209), sourced from Selleck and MCE.. Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.

    Article Title: Network Pharmacology and Experimental Validation Reveal Aidi Injection Suppresses Prostate Cancer via EGFR Inhibition and STAT3-Mediated Apoptosis.
    Article Snippet: RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO2.RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO2.. The treatments included the following compounds: Quercetin (Selleck, S2391), Kaempferol (MedChemExpress, HY-14590), Isorhamnetin (MedChemExpress, HY-N0776), and Cantharidin (MedChemExpress, HY-N0209), sourced from Selleck and MCE.. Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.

    Article Title: Inhibitory Effect of Phenolic Compounds on Vascular Endothelial Growth Factor-Induced Retinal Endothelial Permeability and Angiogenesis
    Article Snippet: Syringic acid, (−)-epicatechin gallate, (−)-epigallocatechin, (−)-epicatechin, vanillic acid, catechin, protocatechuic acid, sinapinic acid, resveratrol, luteolin, gallic acid, naringenin, ferulic acid, apigenin, caffeic acid, formononetin, biochanin A, galangin, p-coumaric acid, isorhamnetin, kaempferol, myricetin, daidzein, glycitein, tangeretin, hesperetin, cyanidin chloride, genistein, naringin, fisetin, quercetin, narirutin, tannic acid, eriodictyol, sorafenib, and lutein were purchased from MedChemExpress. .. M

    Article Title: Targeting senescence induced by age or chemotherapy with a polyphenol-rich natural extract improves longevity and healthspan in mice.
    Article Snippet: Individual constituents used in Fig. 5b were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.Individual constituents used in Fig. 5b were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.. Tested compounds include 3,4-dicaffeoylquinic acid (MedChem Express, cat. no. HY-N0057), kaempferol (MedChem Express, cat. no. HY-14590), isorhamnetin (MedChem Express, cat. no. HY-N0776), isoquercetin (MedChem Express, cat. no. HY-N1445), luteolin (Tocris Bioscience, cat. no. 2874), luteolin-7-O-glucuronide (MedChem Express, cat. no. HY-N1463), nobiletin (MedChem Express, cat. no. HY-N015) and rosmarinic acid (MedChem Express, cat. no. HY-N0529).

    Article Title: Targeting senescence induced by age or chemotherapy with a polyphenol-rich natural extract improves longevity and healthspan in mice
    Article Snippet: Individual constituents used in Fig. were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.Individual constituents used in Fig. were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.. Tested compounds include 3,4-dicaffeoylquinic acid (MedChem Express, cat. no. HY-N0057), kaempferol (MedChem Express, cat. no. HY-14590), isorhamnetin (MedChem Express, cat. no. HY-N0776), isoquercetin (MedChem Express, cat. no. HY-N1445), luteolin (Tocris Bioscience, cat. no. 2874), luteolin-7-O-glucuronide (MedChem Express, cat. no. HY-N1463), nobiletin (MedChem Express, cat. no. HY-N015) and rosmarinic acid (MedChem Express, cat. no. HY-N0529).

    Control:

    Article Title: Isorhamnetin from Astragalus membranaceus exerts anti-glioma effects via PI3K/Akt signaling pathway
    Article Snippet: The Cell Counting Kit 8 (CCK8) assay was obtained from Beyotime (USA).The Cell Counting Kit 8 (CCK8) assay was obtained from Beyotime (USA).. Isorhamnetin was acquired from MedChemExpress (MCE, https://www.medchemexpress.com).. The Annexin V/PI apoptosis detection kit was also obtained from Beyotime (Shanghai, China).The Annexin V/PI apoptosis detection kit was also obtained from Beyotime (Shanghai, China).

    Article Title: Network Pharmacology and Experimental Validation Reveal Aidi Injection Suppresses Prostate Cancer via EGFR Inhibition and STAT3-Mediated Apoptosis.
    Article Snippet: he following compounds: Quercetin (Selleck, S2391), Kaempferol (MedChemExpress, HY-14590), Isorhamnetin (MedChemExpress, HY-N0776), and Cantharidin (MedChemExpress, HY-N0209), sourced from Selleck and MCE. Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20℃. The CCK-8 and DAPI kits were from Solarbio Life Sciences (Beijing, China), while the IP-10 (CXCL10) Mouse ELISA K

    Article Title: Network Pharmacology and Experimental Validation Reveal Aidi Injection Suppresses Prostate Cancer via EGFR Inhibition and STAT3-Mediated Apoptosis
    Article Snippet: RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO 2 .RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO 2 .. The treatments included the following compounds: Quercetin (Selleck, S2391), Kaempferol (MedChemExpress, HY-14590), Isorhamnetin (MedChemExpress, HY-N0776), and Cantharidin (MedChemExpress, HY-N0209), sourced from Selleck and MCE.. Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.

    Article Title: Network Pharmacology and Experimental Validation Reveal Aidi Injection Suppresses Prostate Cancer via EGFR Inhibition and STAT3-Mediated Apoptosis.
    Article Snippet: RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO2.RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO2.. The treatments included the following compounds: Quercetin (Selleck, S2391), Kaempferol (MedChemExpress, HY-14590), Isorhamnetin (MedChemExpress, HY-N0776), and Cantharidin (MedChemExpress, HY-N0209), sourced from Selleck and MCE.. Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.

    Article Title: Inhibitory Effect of Phenolic Compounds on Vascular Endothelial Growth Factor-Induced Retinal Endothelial Permeability and Angiogenesis
    Article Snippet: Syringic acid, (−)-epicatechin gallate, (−)-epigallocatechin, (−)-epicatechin, vanillic acid, catechin, protocatechuic acid, sinapinic acid, resveratrol, luteolin, gallic acid, naringenin, ferulic acid, apigenin, caffeic acid, formononetin, biochanin A, galangin, p-coumaric acid, isorhamnetin, kaempferol, myricetin, daidzein, glycitein, tangeretin, hesperetin, cyanidin chloride, genistein, naringin, fisetin, quercetin, narirutin, tannic acid, eriodictyol, sorafenib, and lutein were purchased from MedChemExpress. .. M

    Article Title: Targeting senescence induced by age or chemotherapy with a polyphenol-rich natural extract improves longevity and healthspan in mice.
    Article Snippet: Individual constituents used in Fig. 5b were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.Individual constituents used in Fig. 5b were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.. Tested compounds include 3,4-dicaffeoylquinic acid (MedChem Express, cat. no. HY-N0057), kaempferol (MedChem Express, cat. no. HY-14590), isorhamnetin (MedChem Express, cat. no. HY-N0776), isoquercetin (MedChem Express, cat. no. HY-N1445), luteolin (Tocris Bioscience, cat. no. 2874), luteolin-7-O-glucuronide (MedChem Express, cat. no. HY-N1463), nobiletin (MedChem Express, cat. no. HY-N015) and rosmarinic acid (MedChem Express, cat. no. HY-N0529).

    Article Title: Targeting senescence induced by age or chemotherapy with a polyphenol-rich natural extract improves longevity and healthspan in mice
    Article Snippet: Individual constituents used in Fig. were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.Individual constituents used in Fig. were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.. Tested compounds include 3,4-dicaffeoylquinic acid (MedChem Express, cat. no. HY-N0057), kaempferol (MedChem Express, cat. no. HY-14590), isorhamnetin (MedChem Express, cat. no. HY-N0776), isoquercetin (MedChem Express, cat. no. HY-N1445), luteolin (Tocris Bioscience, cat. no. 2874), luteolin-7-O-glucuronide (MedChem Express, cat. no. HY-N1463), nobiletin (MedChem Express, cat. no. HY-N015) and rosmarinic acid (MedChem Express, cat. no. HY-N0529).

    Cell Culture:

    Article Title: Isorhamnetin from Astragalus membranaceus exerts anti-glioma effects via PI3K/Akt signaling pathway
    Article Snippet: The Cell Counting Kit 8 (CCK8) assay was obtained from Beyotime (USA).The Cell Counting Kit 8 (CCK8) assay was obtained from Beyotime (USA).. Isorhamnetin was acquired from MedChemExpress (MCE, https://www.medchemexpress.com).. The Annexin V/PI apoptosis detection kit was also obtained from Beyotime (Shanghai, China).The Annexin V/PI apoptosis detection kit was also obtained from Beyotime (Shanghai, China).

    Article Title: Network Pharmacology and Experimental Validation Reveal Aidi Injection Suppresses Prostate Cancer via EGFR Inhibition and STAT3-Mediated Apoptosis.
    Article Snippet: he following compounds: Quercetin (Selleck, S2391), Kaempferol (MedChemExpress, HY-14590), Isorhamnetin (MedChemExpress, HY-N0776), and Cantharidin (MedChemExpress, HY-N0209), sourced from Selleck and MCE. Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20℃. The CCK-8 and DAPI kits were from Solarbio Life Sciences (Beijing, China), while the IP-10 (CXCL10) Mouse ELISA K

    Article Title: Network Pharmacology and Experimental Validation Reveal Aidi Injection Suppresses Prostate Cancer via EGFR Inhibition and STAT3-Mediated Apoptosis
    Article Snippet: RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO 2 .RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO 2 .. The treatments included the following compounds: Quercetin (Selleck, S2391), Kaempferol (MedChemExpress, HY-14590), Isorhamnetin (MedChemExpress, HY-N0776), and Cantharidin (MedChemExpress, HY-N0209), sourced from Selleck and MCE.. Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.

    Article Title: Network Pharmacology and Experimental Validation Reveal Aidi Injection Suppresses Prostate Cancer via EGFR Inhibition and STAT3-Mediated Apoptosis.
    Article Snippet: RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO2.RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO2.. The treatments included the following compounds: Quercetin (Selleck, S2391), Kaempferol (MedChemExpress, HY-14590), Isorhamnetin (MedChemExpress, HY-N0776), and Cantharidin (MedChemExpress, HY-N0209), sourced from Selleck and MCE.. Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.

    Article Title: Inhibitory Effect of Phenolic Compounds on Vascular Endothelial Growth Factor-Induced Retinal Endothelial Permeability and Angiogenesis
    Article Snippet: Syringic acid, (−)-epicatechin gallate, (−)-epigallocatechin, (−)-epicatechin, vanillic acid, catechin, protocatechuic acid, sinapinic acid, resveratrol, luteolin, gallic acid, naringenin, ferulic acid, apigenin, caffeic acid, formononetin, biochanin A, galangin, p-coumaric acid, isorhamnetin, kaempferol, myricetin, daidzein, glycitein, tangeretin, hesperetin, cyanidin chloride, genistein, naringin, fisetin, quercetin, narirutin, tannic acid, eriodictyol, sorafenib, and lutein were purchased from MedChemExpress. .. M

    Article Title: Targeting senescence induced by age or chemotherapy with a polyphenol-rich natural extract improves longevity and healthspan in mice.
    Article Snippet: Individual constituents used in Fig. 5b were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.Individual constituents used in Fig. 5b were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.. Tested compounds include 3,4-dicaffeoylquinic acid (MedChem Express, cat. no. HY-N0057), kaempferol (MedChem Express, cat. no. HY-14590), isorhamnetin (MedChem Express, cat. no. HY-N0776), isoquercetin (MedChem Express, cat. no. HY-N1445), luteolin (Tocris Bioscience, cat. no. 2874), luteolin-7-O-glucuronide (MedChem Express, cat. no. HY-N1463), nobiletin (MedChem Express, cat. no. HY-N015) and rosmarinic acid (MedChem Express, cat. no. HY-N0529).

    Article Title: Targeting senescence induced by age or chemotherapy with a polyphenol-rich natural extract improves longevity and healthspan in mice
    Article Snippet: Individual constituents used in Fig. were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.Individual constituents used in Fig. were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.. Tested compounds include 3,4-dicaffeoylquinic acid (MedChem Express, cat. no. HY-N0057), kaempferol (MedChem Express, cat. no. HY-14590), isorhamnetin (MedChem Express, cat. no. HY-N0776), isoquercetin (MedChem Express, cat. no. HY-N1445), luteolin (Tocris Bioscience, cat. no. 2874), luteolin-7-O-glucuronide (MedChem Express, cat. no. HY-N1463), nobiletin (MedChem Express, cat. no. HY-N015) and rosmarinic acid (MedChem Express, cat. no. HY-N0529).

    Concentration Assay:

    Article Title: Isorhamnetin from Astragalus membranaceus exerts anti-glioma effects via PI3K/Akt signaling pathway
    Article Snippet: The Cell Counting Kit 8 (CCK8) assay was obtained from Beyotime (USA).The Cell Counting Kit 8 (CCK8) assay was obtained from Beyotime (USA).. Isorhamnetin was acquired from MedChemExpress (MCE, https://www.medchemexpress.com).. The Annexin V/PI apoptosis detection kit was also obtained from Beyotime (Shanghai, China).The Annexin V/PI apoptosis detection kit was also obtained from Beyotime (Shanghai, China).

    Article Title: Network Pharmacology and Experimental Validation Reveal Aidi Injection Suppresses Prostate Cancer via EGFR Inhibition and STAT3-Mediated Apoptosis.
    Article Snippet: he following compounds: Quercetin (Selleck, S2391), Kaempferol (MedChemExpress, HY-14590), Isorhamnetin (MedChemExpress, HY-N0776), and Cantharidin (MedChemExpress, HY-N0209), sourced from Selleck and MCE. Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20℃. The CCK-8 and DAPI kits were from Solarbio Life Sciences (Beijing, China), while the IP-10 (CXCL10) Mouse ELISA K

    Article Title: Network Pharmacology and Experimental Validation Reveal Aidi Injection Suppresses Prostate Cancer via EGFR Inhibition and STAT3-Mediated Apoptosis
    Article Snippet: RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO 2 .RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO 2 .. The treatments included the following compounds: Quercetin (Selleck, S2391), Kaempferol (MedChemExpress, HY-14590), Isorhamnetin (MedChemExpress, HY-N0776), and Cantharidin (MedChemExpress, HY-N0209), sourced from Selleck and MCE.. Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.

    Article Title: Network Pharmacology and Experimental Validation Reveal Aidi Injection Suppresses Prostate Cancer via EGFR Inhibition and STAT3-Mediated Apoptosis.
    Article Snippet: RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO2.RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO2.. The treatments included the following compounds: Quercetin (Selleck, S2391), Kaempferol (MedChemExpress, HY-14590), Isorhamnetin (MedChemExpress, HY-N0776), and Cantharidin (MedChemExpress, HY-N0209), sourced from Selleck and MCE.. Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.

    Article Title: Inhibitory Effect of Phenolic Compounds on Vascular Endothelial Growth Factor-Induced Retinal Endothelial Permeability and Angiogenesis
    Article Snippet: Syringic acid, (−)-epicatechin gallate, (−)-epigallocatechin, (−)-epicatechin, vanillic acid, catechin, protocatechuic acid, sinapinic acid, resveratrol, luteolin, gallic acid, naringenin, ferulic acid, apigenin, caffeic acid, formononetin, biochanin A, galangin, p-coumaric acid, isorhamnetin, kaempferol, myricetin, daidzein, glycitein, tangeretin, hesperetin, cyanidin chloride, genistein, naringin, fisetin, quercetin, narirutin, tannic acid, eriodictyol, sorafenib, and lutein were purchased from MedChemExpress. .. M

    Article Title: Targeting senescence induced by age or chemotherapy with a polyphenol-rich natural extract improves longevity and healthspan in mice.
    Article Snippet: Individual constituents used in Fig. 5b were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.Individual constituents used in Fig. 5b were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.. Tested compounds include 3,4-dicaffeoylquinic acid (MedChem Express, cat. no. HY-N0057), kaempferol (MedChem Express, cat. no. HY-14590), isorhamnetin (MedChem Express, cat. no. HY-N0776), isoquercetin (MedChem Express, cat. no. HY-N1445), luteolin (Tocris Bioscience, cat. no. 2874), luteolin-7-O-glucuronide (MedChem Express, cat. no. HY-N1463), nobiletin (MedChem Express, cat. no. HY-N015) and rosmarinic acid (MedChem Express, cat. no. HY-N0529).

    Article Title: Targeting senescence induced by age or chemotherapy with a polyphenol-rich natural extract improves longevity and healthspan in mice
    Article Snippet: Individual constituents used in Fig. were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.Individual constituents used in Fig. were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.. Tested compounds include 3,4-dicaffeoylquinic acid (MedChem Express, cat. no. HY-N0057), kaempferol (MedChem Express, cat. no. HY-14590), isorhamnetin (MedChem Express, cat. no. HY-N0776), isoquercetin (MedChem Express, cat. no. HY-N1445), luteolin (Tocris Bioscience, cat. no. 2874), luteolin-7-O-glucuronide (MedChem Express, cat. no. HY-N1463), nobiletin (MedChem Express, cat. no. HY-N015) and rosmarinic acid (MedChem Express, cat. no. HY-N0529).

    Irradiation:

    Article Title: Isorhamnetin from Astragalus membranaceus exerts anti-glioma effects via PI3K/Akt signaling pathway
    Article Snippet: The Cell Counting Kit 8 (CCK8) assay was obtained from Beyotime (USA).The Cell Counting Kit 8 (CCK8) assay was obtained from Beyotime (USA).. Isorhamnetin was acquired from MedChemExpress (MCE, https://www.medchemexpress.com).. The Annexin V/PI apoptosis detection kit was also obtained from Beyotime (Shanghai, China).The Annexin V/PI apoptosis detection kit was also obtained from Beyotime (Shanghai, China).

    Article Title: Network Pharmacology and Experimental Validation Reveal Aidi Injection Suppresses Prostate Cancer via EGFR Inhibition and STAT3-Mediated Apoptosis.
    Article Snippet: he following compounds: Quercetin (Selleck, S2391), Kaempferol (MedChemExpress, HY-14590), Isorhamnetin (MedChemExpress, HY-N0776), and Cantharidin (MedChemExpress, HY-N0209), sourced from Selleck and MCE. Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20℃. The CCK-8 and DAPI kits were from Solarbio Life Sciences (Beijing, China), while the IP-10 (CXCL10) Mouse ELISA K

    Article Title: Network Pharmacology and Experimental Validation Reveal Aidi Injection Suppresses Prostate Cancer via EGFR Inhibition and STAT3-Mediated Apoptosis
    Article Snippet: RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO 2 .RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO 2 .. The treatments included the following compounds: Quercetin (Selleck, S2391), Kaempferol (MedChemExpress, HY-14590), Isorhamnetin (MedChemExpress, HY-N0776), and Cantharidin (MedChemExpress, HY-N0209), sourced from Selleck and MCE.. Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.

    Article Title: Network Pharmacology and Experimental Validation Reveal Aidi Injection Suppresses Prostate Cancer via EGFR Inhibition and STAT3-Mediated Apoptosis.
    Article Snippet: RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO2.RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO2.. The treatments included the following compounds: Quercetin (Selleck, S2391), Kaempferol (MedChemExpress, HY-14590), Isorhamnetin (MedChemExpress, HY-N0776), and Cantharidin (MedChemExpress, HY-N0209), sourced from Selleck and MCE.. Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.

    Article Title: Inhibitory Effect of Phenolic Compounds on Vascular Endothelial Growth Factor-Induced Retinal Endothelial Permeability and Angiogenesis
    Article Snippet: Syringic acid, (−)-epicatechin gallate, (−)-epigallocatechin, (−)-epicatechin, vanillic acid, catechin, protocatechuic acid, sinapinic acid, resveratrol, luteolin, gallic acid, naringenin, ferulic acid, apigenin, caffeic acid, formononetin, biochanin A, galangin, p-coumaric acid, isorhamnetin, kaempferol, myricetin, daidzein, glycitein, tangeretin, hesperetin, cyanidin chloride, genistein, naringin, fisetin, quercetin, narirutin, tannic acid, eriodictyol, sorafenib, and lutein were purchased from MedChemExpress. .. M

    Article Title: Targeting senescence induced by age or chemotherapy with a polyphenol-rich natural extract improves longevity and healthspan in mice.
    Article Snippet: Individual constituents used in Fig. 5b were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.Individual constituents used in Fig. 5b were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.. Tested compounds include 3,4-dicaffeoylquinic acid (MedChem Express, cat. no. HY-N0057), kaempferol (MedChem Express, cat. no. HY-14590), isorhamnetin (MedChem Express, cat. no. HY-N0776), isoquercetin (MedChem Express, cat. no. HY-N1445), luteolin (Tocris Bioscience, cat. no. 2874), luteolin-7-O-glucuronide (MedChem Express, cat. no. HY-N1463), nobiletin (MedChem Express, cat. no. HY-N015) and rosmarinic acid (MedChem Express, cat. no. HY-N0529).

    Article Title: Targeting senescence induced by age or chemotherapy with a polyphenol-rich natural extract improves longevity and healthspan in mice
    Article Snippet: Individual constituents used in Fig. were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.Individual constituents used in Fig. were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.. Tested compounds include 3,4-dicaffeoylquinic acid (MedChem Express, cat. no. HY-N0057), kaempferol (MedChem Express, cat. no. HY-14590), isorhamnetin (MedChem Express, cat. no. HY-N0776), isoquercetin (MedChem Express, cat. no. HY-N1445), luteolin (Tocris Bioscience, cat. no. 2874), luteolin-7-O-glucuronide (MedChem Express, cat. no. HY-N1463), nobiletin (MedChem Express, cat. no. HY-N015) and rosmarinic acid (MedChem Express, cat. no. HY-N0529).

    Clinical Proteomics:

    Article Title: Isorhamnetin from Astragalus membranaceus exerts anti-glioma effects via PI3K/Akt signaling pathway
    Article Snippet: The Cell Counting Kit 8 (CCK8) assay was obtained from Beyotime (USA).The Cell Counting Kit 8 (CCK8) assay was obtained from Beyotime (USA).. Isorhamnetin was acquired from MedChemExpress (MCE, https://www.medchemexpress.com).. The Annexin V/PI apoptosis detection kit was also obtained from Beyotime (Shanghai, China).The Annexin V/PI apoptosis detection kit was also obtained from Beyotime (Shanghai, China).

    Article Title: Network Pharmacology and Experimental Validation Reveal Aidi Injection Suppresses Prostate Cancer via EGFR Inhibition and STAT3-Mediated Apoptosis.
    Article Snippet: he following compounds: Quercetin (Selleck, S2391), Kaempferol (MedChemExpress, HY-14590), Isorhamnetin (MedChemExpress, HY-N0776), and Cantharidin (MedChemExpress, HY-N0209), sourced from Selleck and MCE. Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20℃. The CCK-8 and DAPI kits were from Solarbio Life Sciences (Beijing, China), while the IP-10 (CXCL10) Mouse ELISA K

    Article Title: Network Pharmacology and Experimental Validation Reveal Aidi Injection Suppresses Prostate Cancer via EGFR Inhibition and STAT3-Mediated Apoptosis
    Article Snippet: RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO 2 .RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO 2 .. The treatments included the following compounds: Quercetin (Selleck, S2391), Kaempferol (MedChemExpress, HY-14590), Isorhamnetin (MedChemExpress, HY-N0776), and Cantharidin (MedChemExpress, HY-N0209), sourced from Selleck and MCE.. Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.

    Article Title: Network Pharmacology and Experimental Validation Reveal Aidi Injection Suppresses Prostate Cancer via EGFR Inhibition and STAT3-Mediated Apoptosis.
    Article Snippet: RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO2.RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO2.. The treatments included the following compounds: Quercetin (Selleck, S2391), Kaempferol (MedChemExpress, HY-14590), Isorhamnetin (MedChemExpress, HY-N0776), and Cantharidin (MedChemExpress, HY-N0209), sourced from Selleck and MCE.. Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.

    Article Title: Inhibitory Effect of Phenolic Compounds on Vascular Endothelial Growth Factor-Induced Retinal Endothelial Permeability and Angiogenesis
    Article Snippet: Syringic acid, (−)-epicatechin gallate, (−)-epigallocatechin, (−)-epicatechin, vanillic acid, catechin, protocatechuic acid, sinapinic acid, resveratrol, luteolin, gallic acid, naringenin, ferulic acid, apigenin, caffeic acid, formononetin, biochanin A, galangin, p-coumaric acid, isorhamnetin, kaempferol, myricetin, daidzein, glycitein, tangeretin, hesperetin, cyanidin chloride, genistein, naringin, fisetin, quercetin, narirutin, tannic acid, eriodictyol, sorafenib, and lutein were purchased from MedChemExpress. .. M

    Article Title: Targeting senescence induced by age or chemotherapy with a polyphenol-rich natural extract improves longevity and healthspan in mice.
    Article Snippet: Individual constituents used in Fig. 5b were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.Individual constituents used in Fig. 5b were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.. Tested compounds include 3,4-dicaffeoylquinic acid (MedChem Express, cat. no. HY-N0057), kaempferol (MedChem Express, cat. no. HY-14590), isorhamnetin (MedChem Express, cat. no. HY-N0776), isoquercetin (MedChem Express, cat. no. HY-N1445), luteolin (Tocris Bioscience, cat. no. 2874), luteolin-7-O-glucuronide (MedChem Express, cat. no. HY-N1463), nobiletin (MedChem Express, cat. no. HY-N015) and rosmarinic acid (MedChem Express, cat. no. HY-N0529).

    Article Title: Targeting senescence induced by age or chemotherapy with a polyphenol-rich natural extract improves longevity and healthspan in mice
    Article Snippet: Individual constituents used in Fig. were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.Individual constituents used in Fig. were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.. Tested compounds include 3,4-dicaffeoylquinic acid (MedChem Express, cat. no. HY-N0057), kaempferol (MedChem Express, cat. no. HY-14590), isorhamnetin (MedChem Express, cat. no. HY-N0776), isoquercetin (MedChem Express, cat. no. HY-N1445), luteolin (Tocris Bioscience, cat. no. 2874), luteolin-7-O-glucuronide (MedChem Express, cat. no. HY-N1463), nobiletin (MedChem Express, cat. no. HY-N015) and rosmarinic acid (MedChem Express, cat. no. HY-N0529).

    Staining:

    Article Title: Isorhamnetin from Astragalus membranaceus exerts anti-glioma effects via PI3K/Akt signaling pathway
    Article Snippet: The Cell Counting Kit 8 (CCK8) assay was obtained from Beyotime (USA).The Cell Counting Kit 8 (CCK8) assay was obtained from Beyotime (USA).. Isorhamnetin was acquired from MedChemExpress (MCE, https://www.medchemexpress.com).. The Annexin V/PI apoptosis detection kit was also obtained from Beyotime (Shanghai, China).The Annexin V/PI apoptosis detection kit was also obtained from Beyotime (Shanghai, China).

    Article Title: Network Pharmacology and Experimental Validation Reveal Aidi Injection Suppresses Prostate Cancer via EGFR Inhibition and STAT3-Mediated Apoptosis.
    Article Snippet: he following compounds: Quercetin (Selleck, S2391), Kaempferol (MedChemExpress, HY-14590), Isorhamnetin (MedChemExpress, HY-N0776), and Cantharidin (MedChemExpress, HY-N0209), sourced from Selleck and MCE. Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20℃. The CCK-8 and DAPI kits were from Solarbio Life Sciences (Beijing, China), while the IP-10 (CXCL10) Mouse ELISA K

    Article Title: Network Pharmacology and Experimental Validation Reveal Aidi Injection Suppresses Prostate Cancer via EGFR Inhibition and STAT3-Mediated Apoptosis
    Article Snippet: RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO 2 .RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO 2 .. The treatments included the following compounds: Quercetin (Selleck, S2391), Kaempferol (MedChemExpress, HY-14590), Isorhamnetin (MedChemExpress, HY-N0776), and Cantharidin (MedChemExpress, HY-N0209), sourced from Selleck and MCE.. Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.

    Article Title: Network Pharmacology and Experimental Validation Reveal Aidi Injection Suppresses Prostate Cancer via EGFR Inhibition and STAT3-Mediated Apoptosis.
    Article Snippet: RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO2.RM-1, DU145, and BPH-1 cell lines were obtained from the Chinese Academy of Sciences (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM, HyClone) with 10% fetal bovine serum (FBS, HyClone) at 37°C in a humidified incubator with 95% air and 5% CO2.. The treatments included the following compounds: Quercetin (Selleck, S2391), Kaempferol (MedChemExpress, HY-14590), Isorhamnetin (MedChemExpress, HY-N0776), and Cantharidin (MedChemExpress, HY-N0209), sourced from Selleck and MCE.. Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.Each was dissolved in dimethyl sulfoxide (DMSO) at 100 mmol/L and stored at −20°C.

    Article Title: Inhibitory Effect of Phenolic Compounds on Vascular Endothelial Growth Factor-Induced Retinal Endothelial Permeability and Angiogenesis
    Article Snippet: Syringic acid, (−)-epicatechin gallate, (−)-epigallocatechin, (−)-epicatechin, vanillic acid, catechin, protocatechuic acid, sinapinic acid, resveratrol, luteolin, gallic acid, naringenin, ferulic acid, apigenin, caffeic acid, formononetin, biochanin A, galangin, p-coumaric acid, isorhamnetin, kaempferol, myricetin, daidzein, glycitein, tangeretin, hesperetin, cyanidin chloride, genistein, naringin, fisetin, quercetin, narirutin, tannic acid, eriodictyol, sorafenib, and lutein were purchased from MedChemExpress. .. M

    Article Title: Targeting senescence induced by age or chemotherapy with a polyphenol-rich natural extract improves longevity and healthspan in mice.
    Article Snippet: Individual constituents used in Fig. 5b were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.Individual constituents used in Fig. 5b were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.. Tested compounds include 3,4-dicaffeoylquinic acid (MedChem Express, cat. no. HY-N0057), kaempferol (MedChem Express, cat. no. HY-14590), isorhamnetin (MedChem Express, cat. no. HY-N0776), isoquercetin (MedChem Express, cat. no. HY-N1445), luteolin (Tocris Bioscience, cat. no. 2874), luteolin-7-O-glucuronide (MedChem Express, cat. no. HY-N1463), nobiletin (MedChem Express, cat. no. HY-N015) and rosmarinic acid (MedChem Express, cat. no. HY-N0529).

    Article Title: Targeting senescence induced by age or chemotherapy with a polyphenol-rich natural extract improves longevity and healthspan in mice
    Article Snippet: Individual constituents used in Fig. were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.Individual constituents used in Fig. were dissolved in DMSO and adjusted to a final concentration of 1 μM in MEMα for treatment.. Tested compounds include 3,4-dicaffeoylquinic acid (MedChem Express, cat. no. HY-N0057), kaempferol (MedChem Express, cat. no. HY-14590), isorhamnetin (MedChem Express, cat. no. HY-N0776), isoquercetin (MedChem Express, cat. no. HY-N1445), luteolin (Tocris Bioscience, cat. no. 2874), luteolin-7-O-glucuronide (MedChem Express, cat. no. HY-N1463), nobiletin (MedChem Express, cat. no. HY-N015) and rosmarinic acid (MedChem Express, cat. no. HY-N0529).



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    Results of Bibliometric Analysis on Isorhamnetin in Tumor Research. A. Publication trends of isorhamnetin-related studies over the past decade. B. Co-occurrence network of research themes in isorhamnetin studies. C. Annual publication count of isorhamnetin-related cancer research (2014-2024). D. Top 10 keywords with strongest citation bursts: blue bars indicate keyword appearance periods, red bars denote burst periods. E. Keyword co-occurrence analysis.

    Journal: American Journal of Cancer Research

    Article Title: Isorhamnetin from Astragalus membranaceus exerts anti-glioma effects via PI3K/Akt signaling pathway

    doi: 10.62347/XWBV2926

    Figure Lengend Snippet: Results of Bibliometric Analysis on Isorhamnetin in Tumor Research. A. Publication trends of isorhamnetin-related studies over the past decade. B. Co-occurrence network of research themes in isorhamnetin studies. C. Annual publication count of isorhamnetin-related cancer research (2014-2024). D. Top 10 keywords with strongest citation bursts: blue bars indicate keyword appearance periods, red bars denote burst periods. E. Keyword co-occurrence analysis.

    Article Snippet: Isorhamnetin was acquired from MedChemExpress (MCE, https://www.medchemexpress.com).

    Techniques:

    Molecular docking diagram of isorhamnetin and target molecules in the PI3K-Akt pathway.

    Journal: American Journal of Cancer Research

    Article Title: Isorhamnetin from Astragalus membranaceus exerts anti-glioma effects via PI3K/Akt signaling pathway

    doi: 10.62347/XWBV2926

    Figure Lengend Snippet: Molecular docking diagram of isorhamnetin and target molecules in the PI3K-Akt pathway.

    Article Snippet: Isorhamnetin was acquired from MedChemExpress (MCE, https://www.medchemexpress.com).

    Techniques:

    Impact of isorhamnetin on glioma cell viability and proliferation. (A, B) CCK8 assay results for U87MG (A) and U251 (B) cells exposed to varying isorhamnetin concentrations (0, 25, 50, 100 μM) over 48, 72, and 96 hours. (C, D) U87 and U251 cells underwent clonogenic assays under a 4× magnification microscope (C) and quantitative analysis (n = 3) (D) after treatment with varying concentrations of isorhamnetin (0, 25, 50, 100 μM). (E, F) Flow cytometry was used to assess apoptosis in U87 and U251 cells exposed to varying isorhamnetin concentrations (0, 25, 50, 100 μM) for 48 hours (E), followed by quantitative analysis (n = 3) (F).

    Journal: American Journal of Cancer Research

    Article Title: Isorhamnetin from Astragalus membranaceus exerts anti-glioma effects via PI3K/Akt signaling pathway

    doi: 10.62347/XWBV2926

    Figure Lengend Snippet: Impact of isorhamnetin on glioma cell viability and proliferation. (A, B) CCK8 assay results for U87MG (A) and U251 (B) cells exposed to varying isorhamnetin concentrations (0, 25, 50, 100 μM) over 48, 72, and 96 hours. (C, D) U87 and U251 cells underwent clonogenic assays under a 4× magnification microscope (C) and quantitative analysis (n = 3) (D) after treatment with varying concentrations of isorhamnetin (0, 25, 50, 100 μM). (E, F) Flow cytometry was used to assess apoptosis in U87 and U251 cells exposed to varying isorhamnetin concentrations (0, 25, 50, 100 μM) for 48 hours (E), followed by quantitative analysis (n = 3) (F).

    Article Snippet: Isorhamnetin was acquired from MedChemExpress (MCE, https://www.medchemexpress.com).

    Techniques: CCK-8 Assay, Microscopy, Flow Cytometry

    Effects of isorhamnetin on glioblastoma cell invasion and migration. (A-D) Images from wound healing assays after 48-hour treatment with varying isorhamnetin concentrations (0, 25, 50, 100 μM) isorhamnetin of U87 (A) and U251 (B) cells under a 4× magnification microscope, along with quantitative analysis of wound healing areas for U87 (C) and U251 (D) cells. (E-H) Images from Transwell assays showing U87 (E) and U251 (F) cells treated with 50 μM isorhamnetin for 48 h under a 10× magnification microscope, and quantitative analysis of migrating U87 (G) cells and U251 (H) cells ( n = 3/group, t -test).

    Journal: American Journal of Cancer Research

    Article Title: Isorhamnetin from Astragalus membranaceus exerts anti-glioma effects via PI3K/Akt signaling pathway

    doi: 10.62347/XWBV2926

    Figure Lengend Snippet: Effects of isorhamnetin on glioblastoma cell invasion and migration. (A-D) Images from wound healing assays after 48-hour treatment with varying isorhamnetin concentrations (0, 25, 50, 100 μM) isorhamnetin of U87 (A) and U251 (B) cells under a 4× magnification microscope, along with quantitative analysis of wound healing areas for U87 (C) and U251 (D) cells. (E-H) Images from Transwell assays showing U87 (E) and U251 (F) cells treated with 50 μM isorhamnetin for 48 h under a 10× magnification microscope, and quantitative analysis of migrating U87 (G) cells and U251 (H) cells ( n = 3/group, t -test).

    Article Snippet: Isorhamnetin was acquired from MedChemExpress (MCE, https://www.medchemexpress.com).

    Techniques: Migration, Microscopy

    WB analysis of isorhamnetin’s inhibition of the PI3K/Akt signaling pathway in glioma cells. (A-D) WB analysis was conducted to assess the expression levels of PI3K, p-PI3K, Akt, and p-Akt in U87 and U251 cells following 48-hour treatment with isorhamnetin at concentrations of 0, 25, 50, and 100 μM (A, C), along with corresponding quantitative evaluations (B, D). (E, F) The protein levels of Bcl-2 and Bax were examined in the same cell lines and WB conditions (E, G), accompanied by quantitative analysis (F, H).

    Journal: American Journal of Cancer Research

    Article Title: Isorhamnetin from Astragalus membranaceus exerts anti-glioma effects via PI3K/Akt signaling pathway

    doi: 10.62347/XWBV2926

    Figure Lengend Snippet: WB analysis of isorhamnetin’s inhibition of the PI3K/Akt signaling pathway in glioma cells. (A-D) WB analysis was conducted to assess the expression levels of PI3K, p-PI3K, Akt, and p-Akt in U87 and U251 cells following 48-hour treatment with isorhamnetin at concentrations of 0, 25, 50, and 100 μM (A, C), along with corresponding quantitative evaluations (B, D). (E, F) The protein levels of Bcl-2 and Bax were examined in the same cell lines and WB conditions (E, G), accompanied by quantitative analysis (F, H).

    Article Snippet: Isorhamnetin was acquired from MedChemExpress (MCE, https://www.medchemexpress.com).

    Techniques: Inhibition, Expressing