leonurine (MedChemExpress)
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94
Structured Review
MedChemExpress
leonurine
Leonurine, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hy-n0741/Leonurine/pmc12320140-0-0-4
Average 94 stars, based on 9 article reviews
Leonurine, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hy-n0741/Leonurine/pmc12320140-0-0-4
Average 94 stars, based on 9 article reviews
leonurine - by Bioz Stars,
2026-10
94/100 stars
Images
1) Product Images from "Leonurine Attenuates CCl 4 -Induced Hepatic Fibrosis in Mice via the Hippo-YAP Pathway"
Article Title: Leonurine Attenuates CCl 4 -Induced Hepatic Fibrosis in Mice via the Hippo-YAP Pathway
Journal: Drug Design, Development and Therapy
doi: 10.2147/DDDT.S518793
Figure Legend Snippet: Two-dimensional structure ( A ) and three-dimensional ( B ) structure of Leonurine.
Techniques Used:
Figure Legend Snippet: Leonurine effectively alleviated liver injury caused by CCl 4 . ( A ) C57BL/6 modeling diagram. ( B ) Body weight change in mice. ( C ) Final body weight (g). ( D ) Liver weight (g). ( E ) Liver index (%). ( F ) Macroscopic (scale bar, 1 cm) and Hematoxylin and Eosin staining images of mouse liver (Red arrows mark inflammatory cells, blue arrows mark vacuolation and necrosis) (scale bar, 100 μm) and quantitative analysis results. ( G-I ) Serum Alanine Aminotransferase ( G ), Aspartate Aminotransferase ( H ), and Liver Lactate Dehydrogenase ( I ) in mice. Data are expressed as mean ± standard deviation. **P<0.01, ***P<0.001, ****P<0.0001.
Techniques Used: Staining, Standard Deviation
Figure Legend Snippet: Leonurine inhibits the expression of inflammatory factors in the liver. ( A-C ) Serum inflammatory cytokines TNF-α ( A ), IL-6 ( B ) and IL-1β ( C ) were determined by ELISA. ( D-F ) The mRNA expression levels of TNF-α ( D ), IL-6 ( E ) and IL-1β ( F ) in mouse liver tissue by qRT-PCR. ( G ) Immunohistochemical images and quantitative analysis results of CD68 and F480 (scale bar, 100 μm). Data are expressed as mean ± standard deviation. *P<0.05, **P<0.01, ***P<0.001.
Techniques Used: Expressing, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Immunohistochemical staining, Standard Deviation
Figure Legend Snippet: Leonurine alleviated CCl 4 -induced liver fibrosis in mice. ( A-B ) Masson and Sirius red staining images ( A ) (scale bar, 100 μm) and quantitative analysis results ( B ). ( C ) Hydroxyproline in mouse liver tissue. Data are expressed as mean ± standard deviation. **P<0.01, ***P<0.001, ****P<0.0001.
Techniques Used: Staining, Standard Deviation
Figure Legend Snippet: Leonurine inhibited HSC activation in vivo. ( A ) The mRNA expression levels of COL1A1 and α-SMA in mouse liver tissue by qRT-PCR. ( B ) The protein expression of COL1A1 and α-SMA in mouse liver tissue by Western blotting and quantitative analysis results. ( C ) Immunohistochemical images and quantitative analysis results of α-SMA (scale bar, 100 μm). Data are expressed as mean ± standard deviation. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001.
Techniques Used: Activation Assay, In Vivo, Expressing, Quantitative RT-PCR, Western Blot, Immunohistochemical staining, Standard Deviation
Figure Legend Snippet: Leonurine inhibits HSC activation in vitro by delaying cell proliferation. ( A ) LX-2 cells were pretreated with Leo (1, 2, 5, 10, 20 or 40 μM), and cell viability was measured by CCK-8 assay. ( B ) LX-2 cells were pretreated with Leo (1, 2 or 5 μM) for 24, 48, or 72 h, and cell viability was measured by CCK-8 assay. ( C-D ) The protein expression of CDK2, CyclinE1, CDK4, CyclinD1 and PCNA ( C ) and COL1A1 and α-SMA ( D ) of LX-2 by Western blotting and quantitative analysis results. ( E ) The mRNA expression levels of COL1A1 and α-SMA of LX-2 by qRT-PCR. Data are expressed as mean ± standard deviation. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001.
Techniques Used: Activation Assay, In Vitro, CCK-8 Assay, Expressing, Western Blot, Quantitative RT-PCR, Standard Deviation
Figure Legend Snippet: Leonurine protects the liver by regulating apoptosis. ( A ) The protein expression of Bax, Bcl-2, Caspase-3 of LX-2 by Western blotting and quantitative analysis results. ( B ) The protein expression of Bax, Bcl-2, CHOP and Caspase-3 in mouse liver tissue by Western blotting and quantitative analysis results. ( C ) TUNEL staining and quantification analysis (scale bar, 100 μm). Data are expressed as mean ± standard deviation. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001.
Techniques Used: Expressing, Western Blot, TUNEL Assay, Staining, Standard Deviation
Figure Legend Snippet: Leonurine inhibiting HSC activation by blocking Hippo-YAP signaling pathway. ( A ) The protein expression of YAP1 in mouse liver tissue by Western blotting and quantitative analysis results. ( B ) Immunohistochemical images and quantitative analysis results of YAP1 (scale bar, 100 μm). ( C ) The mRNA expression levels of YAP1, ANKRD1 and CTGF of LX-2 by qRT-PCR. ( D ) The protein expression of Hippo-YAP signaling pathway proteins of LX-2 by Western blotting and quantitative analysis results. ( E ) The protein expression of COL1A1 and α-SMA of LX-2 treated with Leo (5μM) and YAP SiRNA by Western blotting and quantitative analysis results. ( F ) Immunofluorescence staining for YAP1 (green) of LX-2 treated with Leo (5μM) and YAP SiRNA (scale bar, 100 μm). Data are expressed as mean ± standard deviation. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001.
Techniques Used: Activation Assay, Blocking Assay, Expressing, Western Blot, Immunohistochemical staining, Quantitative RT-PCR, Immunofluorescence, Staining, Standard Deviation
Figure Legend Snippet: Schematic representation of Leonurine delaying the progression of liver fibrosis by blocking the Hippo-YAP pathway.
Techniques Used: Blocking Assay
Related Articles
Staining:Article Title: Leonurine Attenuates CCl 4 -Induced Hepatic Fibrosis in Mice via the Hippo-YAP Pathway Article Snippet: Standard Deviation:Article Title: Leonurine Attenuates CCl 4 -Induced Hepatic Fibrosis in Mice via the Hippo-YAP Pathway Article Snippet: Expressing:Article Title: Leonurine Attenuates CCl 4 -Induced Hepatic Fibrosis in Mice via the Hippo-YAP Pathway Article Snippet: Enzyme-linked Immunosorbent Assay:Article Title: Leonurine Attenuates CCl 4 -Induced Hepatic Fibrosis in Mice via the Hippo-YAP Pathway Article Snippet: Quantitative RT-PCR:Article Title: Leonurine Attenuates CCl 4 -Induced Hepatic Fibrosis in Mice via the Hippo-YAP Pathway Article Snippet: Immunohistochemical staining:Article Title: Leonurine Attenuates CCl 4 -Induced Hepatic Fibrosis in Mice via the Hippo-YAP Pathway Article Snippet: Activation Assay:Article Title: Leonurine Attenuates CCl 4 -Induced Hepatic Fibrosis in Mice via the Hippo-YAP Pathway Article Snippet: In Vivo:Article Title: Leonurine Attenuates CCl 4 -Induced Hepatic Fibrosis in Mice via the Hippo-YAP Pathway Article Snippet: Western Blot:Article Title: Leonurine Attenuates CCl 4 -Induced Hepatic Fibrosis in Mice via the Hippo-YAP Pathway Article Snippet: In Vitro:Article Title: Leonurine Attenuates CCl 4 -Induced Hepatic Fibrosis in Mice via the Hippo-YAP Pathway Article Snippet: CCK-8 Assay:Article Title: Leonurine Attenuates CCl 4 -Induced Hepatic Fibrosis in Mice via the Hippo-YAP Pathway Article Snippet: TUNEL Assay:Article Title: Leonurine Attenuates CCl 4 -Induced Hepatic Fibrosis in Mice via the Hippo-YAP Pathway Article Snippet: Blocking Assay:Article Title: Leonurine Attenuates CCl 4 -Induced Hepatic Fibrosis in Mice via the Hippo-YAP Pathway Article Snippet: Immunofluorescence:Article Title: Leonurine Attenuates CCl 4 -Induced Hepatic Fibrosis in Mice via the Hippo-YAP Pathway Article Snippet: Lysis:Article Title: Leonurine Attenuates CCl 4 -Induced Hepatic Fibrosis in Mice via the Hippo-YAP Pathway Article Snippet: |