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MedChemExpress component
Component, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hy-n0716b/Berberine+sulfate/pm38640713-82-10-7
Average 94 stars, based on 9 article reviews

component - by Bioz Stars, 2026-10

94/100 stars

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Cell Culture:

Article Title: Obesity and cervical intraepithelial neoplasia: regulation of mitochondrial energy metabolism via the Kisspeptin/GPR54 signaling pathway.
Article Snippet: .. Cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher, catalog number 11965092) supplemented with 10% fetal bovine serum (FBS, Thermo Fisher, catalog number 16140089) and 100 μg/ml penicillin-streptomycin (Thermo Fisher, catalog number 15070063) under conditions of 37 °C and 5% CO2. (1) The cell lines HCvEpC (EZT-HCEP-1, biosystems), SiHa (HTB-35, ATCC), Caski (CRM-CRL-1550, ATCC), HeLa (CRM-CCL-2, ATCC), and C33A (HTB-31, ATCC) were cultured and assessed for the expression of KISS1R. (2) The treatments for the HeLa and SiHa cell lines included the following: Part 1: (1) sh-NC group: transfection with sh-NC lentivirus for 48 h; (2) sh-KISS1 group: transfection with sh-KISS1 lentivirus for 48 h; (3) oe-NC (overexpression-NC) group: transfection with oe-NC lentivirus for 48 h; (4) oe-KISS1 group: transfection with oe-KISS1 lentivirus for 48 h. Part 2: (1) oe-NC group: transfection with oe-NC lentivirus for 48 h; (2) oe-KISS1 group: transfection with oe-KISS1 lentivirus for 48 h; (3) oe-KISS1 + Ber group: transfection with oe-KISS1 lentivirus for 48 h and treated with 40 μM of Berberine (HY-N0716, MCE) for 48 h [26]. .. Part 3: (1) ctrl group: control group cultured in medium containing 1% bovine serum albumin (BSA); (2) EA group: high-fat simulation group cultured in medium containing 1% EA-bound BSA; (3) EA + sh-NC: cultured in EA-bound BSA medium and transfection with sh-NC lentivirus for 48 h; (4) EA + sh-KISS1: cultured in EA-bound BSA medium and transfection with sh-KISS1 lentivirus for 48 h; (5) EA + sh-KISS1 + PQQ: cultured in EA-bound BSA medium, transfection with sh-KISS1 lentivirus for 48 h, and treated with 10 μM Pyrroloquinoline quinone (PQQ, HY-100196, MCE) for 48 h [27].

Modification:

Expressing:

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Incubation:

Article Title: Mechanistic Language Modeling and Oxygenated 3D Screening Reveal Berberine and Enzalutamide Synergy in Resistant Prostate Cancer
Article Snippet: .. Cells were treated with vehicle, 20 μ M enzalutamide (Enza), 5 μ M berberine (BBR; MedChemExpress, Cat# HY-N0716B), or the combination (Combo) for 8 h. Following treatment, cells were fixed in 4% paraformaldehyde containing phosphatase inhibitor cocktail (Cell Signaling Technology, Cat# 5870S) for 30 min on ice, then permeabilized and blocked in PBS containing 2% donkey serum and 0.25% Triton X-100 at room temperature for 30 min. After PBS washes, primary antibodies against phospho-S6 (p-S6; Cell Signaling Technology, RRID: AB_10694233), total S6 (Cell Signaling Technology, RRID :AB_331355), phospho-AMPK α (p-AMPK α ; Cell Signaling Technology, RRID :AB_2799368), and total AMPK α (Cell Signaling Technology, RRID :AB_10622186) were applied in PBS with 0.125% Triton X-100 and 0.5% BSA and incubated at 4 ◦ C overnight. ..

Drug discovery:

Article Title: Berberine is a Novel Mitochondrial Calcium Uniporter Inhibitor that Disrupts MCU‐EMRE Assembly
Article Snippet: Ru360 (#557440‐1SET, purity: ≥95%) was obtained from Merck (Sigma‐Aldrich, St. Louis, MO, USA). .. Berberine chloride (#HY‐18258, purity: ≥98%), Berberine sulfate (#HY‐N0716B, purity: ≥98%), and the FDA‐approved drug library (HY‐L022) were sourced from MedChemExpress (Monmouth Junction, NJ, USA). ..

Saline:

Article Title: Berberine alleviates neuroinflammation by downregulating NFκB/LCN2 pathway in sepsis-associated encephalopathy: network pharmacology, bioinformatics, and experimental validation.
Article Snippet: Background: Sepsis refers to a systemic inflammatory response caused by infection, involving multiple organs.. Sepsis-associated encephalopathy (SAE), as one of the most common complications in patients with severe sepsis, refers to the diffuse brain dysfunction caused by sepsis without central nervous system infection.. However, there is no clear diagnostic criteria and lack of specific diagnostic markers.

Review