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scc human skin tissues  (Novus Biologicals)


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    Novus Biologicals scc human skin tissues
    Scc Human Skin Tissues, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 85/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+tissue+microarray/Human+Skin+Tissue+MicroArray+(Cancer)/pm40441291-283-24-29
    Average 85 stars, based on 9 article reviews
    scc human skin tissues - by Bioz Stars, 2026-10
    85/100 stars

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    Related Articles

    Immunohistochemical staining:

    Article Title: Up-Regulation of the Lymphatic Marker Podoplanin, a Mucin-Type Transmembrane Glycoprotein, in Human Squamous Cell Carcinomas and Germ Cell Tumors
    Article Snippet: Immunofluorescence stainings were performed on 6-μm cryostat sections of neonatal human foreskin or on 6-μm paraffin sections of human malignant melanoma as described previously, 6,10 using the mouse monoclonal antibody D2-40 (Signet, Dedham, MA), rabbit polyclonal antibodies against the lymphatic markers LYVE-1 7 and Prox1 25 (kindly provided by Dr. K. Alitalo, University of Helsinki, Helsinki, Finland), CD34, CD31 (BD Pharmingen, San Diego, CA), and corresponding secondary antibodies labeled with Alexa Fluor 488 or Alexa Fluor 594 (Molecular Probes, Eugene, OR). .. Additional immunohistochemical stains were performed on tissue arrays of normal mouse (MaxArray mouse tissue microarray slides; Zymed, San Francisco, CA) and human tissues (MaxArray human normal tissue microarray slides, Zymed), human skin tumors (IMH-323; Imgenex, San Diego, CA) and ovary tumors (IMH-347, Imgenex) as described previously. .. 6 Briefly, the primary antibodies D2-40 or LYVE-1 were applied, followed by incubation with conjugated anti-mouse or anti-rabbit immunoglobulin using the 3-amino-9-ethylcabazole peroxidase kit (Vector Laboratories, Burlingame, CA).

    Microarray:

    Article Title: Up-Regulation of the Lymphatic Marker Podoplanin, a Mucin-Type Transmembrane Glycoprotein, in Human Squamous Cell Carcinomas and Germ Cell Tumors
    Article Snippet: Immunofluorescence stainings were performed on 6-μm cryostat sections of neonatal human foreskin or on 6-μm paraffin sections of human malignant melanoma as described previously, 6,10 using the mouse monoclonal antibody D2-40 (Signet, Dedham, MA), rabbit polyclonal antibodies against the lymphatic markers LYVE-1 7 and Prox1 25 (kindly provided by Dr. K. Alitalo, University of Helsinki, Helsinki, Finland), CD34, CD31 (BD Pharmingen, San Diego, CA), and corresponding secondary antibodies labeled with Alexa Fluor 488 or Alexa Fluor 594 (Molecular Probes, Eugene, OR). .. Additional immunohistochemical stains were performed on tissue arrays of normal mouse (MaxArray mouse tissue microarray slides; Zymed, San Francisco, CA) and human tissues (MaxArray human normal tissue microarray slides, Zymed), human skin tumors (IMH-323; Imgenex, San Diego, CA) and ovary tumors (IMH-347, Imgenex) as described previously. .. 6 Briefly, the primary antibodies D2-40 or LYVE-1 were applied, followed by incubation with conjugated anti-mouse or anti-rabbit immunoglobulin using the 3-amino-9-ethylcabazole peroxidase kit (Vector Laboratories, Burlingame, CA).

    Proximity Ligation Assay:

    Article Title: Mitochondrial Raf1 Regulates Glutamine Catabolism
    Article Snippet: .. 736 737 Proximity Ligation Assay (PLA) of tumor tissue microarrays 738 Deparaffinization 739 Proximity ligation of human cancer tissue microarrays (Skin cancer: NBP2-30229, Multiple 740 cancers: NBP2-30263; Novus Biologicals) was used to analyze Raf-GLS protein-protein 741 interaction in diverse cancer contexts. ..

    Ligation:

    Article Title: Mitochondrial Raf1 Regulates Glutamine Catabolism
    Article Snippet: .. 736 737 Proximity Ligation Assay (PLA) of tumor tissue microarrays 738 Deparaffinization 739 Proximity ligation of human cancer tissue microarrays (Skin cancer: NBP2-30229, Multiple 740 cancers: NBP2-30263; Novus Biologicals) was used to analyze Raf-GLS protein-protein 741 interaction in diverse cancer contexts. ..

    other:

    Article Title: Up-Regulation of the Lymphatic Marker Podoplanin, a Mucin-Type Transmembrane Glycoprotein, in Human Squamous Cell Carcinomas and Germ Cell Tumors
    Article Snippet: Nuclei were counterstained with 20 g/ml of Hoechst bisbenzimide (Molecular Probes).

    RNAscope:

    Article Title: In Vivo CRISPR Interference Screen Reveals Long Noncoding RNA Portfolio Crucial for Cutaneous Squamous Cell Carcinoma Tumor Growth.
    Article Snippet: .. Cox proportional hazards regression model fitting cor- rected for sex bias was performed using the survival and adjust- edCurves R packages. smRNA-FISH Healthy and SCC human skin tissues (NBP2-30229, Novus Bi- ologicals) were processed for smRNA-FISH as previously described in Ducoli et al (2021), following the manufacturer’s instruction on the RNAScope Multiplex Fluorescent Reagent kit v2 (Advanced Cell Diagnostics). .. The following RNAscope probes were used: Hs- LINC00704-C1 (411081-C1, Advanced Cell Diagnostics) and Hs- Malat1-C2 (400811-C2, Advanced Cell Diagnostics; as positive control).

    Multiplex Assay:

    Article Title: In Vivo CRISPR Interference Screen Reveals Long Noncoding RNA Portfolio Crucial for Cutaneous Squamous Cell Carcinoma Tumor Growth.
    Article Snippet: .. Cox proportional hazards regression model fitting cor- rected for sex bias was performed using the survival and adjust- edCurves R packages. smRNA-FISH Healthy and SCC human skin tissues (NBP2-30229, Novus Bi- ologicals) were processed for smRNA-FISH as previously described in Ducoli et al (2021), following the manufacturer’s instruction on the RNAScope Multiplex Fluorescent Reagent kit v2 (Advanced Cell Diagnostics). .. The following RNAscope probes were used: Hs- LINC00704-C1 (411081-C1, Advanced Cell Diagnostics) and Hs- Malat1-C2 (400811-C2, Advanced Cell Diagnostics; as positive control).



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    Overexpression of DNPH1 in human breast tumors (A) DNPH1 mRNA expression in various breast cancer subtypes was analyzed in published microarray data. Mean with standard deviation and statistical significance compared to normal breast tissue are indicated; ∗∗∗∗, p < 0.0001 (one-way ANOVA, Tukey’s multiple comparisons test). (B) High (top quartile) DNPH1 mRNA levels predict reduced survival. Data derived from published microarray experiments ; p < 0.0001 (log rank test). (C) DNPH1 protein expression in human breast cell lines assayed by Western blotting. (D) Examples of immunohistochemical staining with DNPH1 antibodies on matching normal and cancerous breast tissue. Scale bars, 0.1 mm. (E) DNPH1 protein expression was measured by immunohistochemistry in 20 matching normal and cancerous human breast tissues; two-tailed, paired t test.

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    doi: 10.1016/j.isci.2026.115227

    Figure Lengend Snippet: Overexpression of DNPH1 in human breast tumors (A) DNPH1 mRNA expression in various breast cancer subtypes was analyzed in published microarray data. Mean with standard deviation and statistical significance compared to normal breast tissue are indicated; ∗∗∗∗, p < 0.0001 (one-way ANOVA, Tukey’s multiple comparisons test). (B) High (top quartile) DNPH1 mRNA levels predict reduced survival. Data derived from published microarray experiments ; p < 0.0001 (log rank test). (C) DNPH1 protein expression in human breast cell lines assayed by Western blotting. (D) Examples of immunohistochemical staining with DNPH1 antibodies on matching normal and cancerous breast tissue. Scale bars, 0.1 mm. (E) DNPH1 protein expression was measured by immunohistochemistry in 20 matching normal and cancerous human breast tissues; two-tailed, paired t test.

    Article Snippet: Human breast tissue microarray , ISU Abxis , A312.

    Techniques: Over Expression, Expressing, Microarray, Standard Deviation, Derivative Assay, Western Blot, Immunohistochemical staining, Staining, Immunohistochemistry, Two Tailed Test

    Myxoid liposarcoma tissues present low levels of CD99 expression. A Representative image of tissue microarray staining of CD99 in Ewing’s sarcoma, liposarcoma myxoid, osteosarcoma, and chondrosarcoma taken with Objective 10 ×. B Quantification of CD99 signal in cancer tissue microarray. Each dot represents one patient-derived sample, where the signal was normalized to Ewing’s sarcoma signal, and then regrouped by cancer type. N = 2 independent experiments were averaged. Ewing’s sarcoma is represented by a dotted line at 100% and serves as a positive control for CD99 abundance level as previously reported

    Journal: BMC Biology

    Article Title: Proteomics analysis of human mesenchymal stromal/stem cell sarcomagenesis model identifies ALDH1A3 and CD99 as potential targets in the transformation process

    doi: 10.1186/s12915-025-02498-z

    Figure Lengend Snippet: Myxoid liposarcoma tissues present low levels of CD99 expression. A Representative image of tissue microarray staining of CD99 in Ewing’s sarcoma, liposarcoma myxoid, osteosarcoma, and chondrosarcoma taken with Objective 10 ×. B Quantification of CD99 signal in cancer tissue microarray. Each dot represents one patient-derived sample, where the signal was normalized to Ewing’s sarcoma signal, and then regrouped by cancer type. N = 2 independent experiments were averaged. Ewing’s sarcoma is represented by a dotted line at 100% and serves as a positive control for CD99 abundance level as previously reported

    Article Snippet: The tissue microarray (#NBP2-30,330) was purchased from Novus Biologicals (Toronto, ON, Canada).

    Techniques: Expressing, Microarray, Staining, Derivative Assay, Positive Control