iv anti hif 1a 1 100 cell signaling technology 48085 (Cell Signaling Technology Inc)

Cell Signaling Technology Inc is a verified supplier

Cell Signaling Technology Inc manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Cell Signaling Technology Inc iv anti hif 1a 1 100 cell signaling technology 48085
Iv Anti Hif 1a 1 100 Cell Signaling Technology 48085, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hif+1a/Prostate+Specific+Membrane+Antigen+XP+Rabbit+mAb/pmc13003023-105-33-38
Average 93 stars, based on 30 article reviews

iv anti hif 1a 1 100 cell signaling technology 48085 - by Bioz Stars, 2026-10

93/100 stars

Images

Virus:

Article Title: BCL2 drives castration resistance in castration-sensitive prostate cancer by orchestrating reciprocal crosstalk between oncogenic pathways.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies BCL2 (124); Mouse Monoclonal Cell Signaling Cat#15071; RRID:AB_2744528 BCl2 (SP66); Rabbit Monoclonal Ventana/Roche Cat#790–4604 PSA/KLK3 (D6B1), Rabbit Monoclonal Cell Signaling Cat5365; RRID:AB_2797609 PSA (Clone ER-PR8) Monoclonal Mouse Anti-Human Agilent/Dako Cat#M075029-2 BCL-W (31H4); Rabbit Monoclonal Cell Signaling Cat#2724; RRID:AB_10691557 BCL-XL(54H6); Rabbit Monoclonal Cell Signaling Cat#2764; RRID:AB_2228008 RHOGDI; Rabbit Polyclonal Santacruz Cat#SC360; RRID:AB_2227516 RHOGDI; Rabbit Polyclonal Cell Signaling Cat#2564; RRID:AB_2274313 androgen receptor; Rabbit Polyclonal Millipore/Sigma Cat#06–680;RRID:AB_310214 Androgen Receptor (D6F11): Rabbit Monoclonal Cell Signaling Cat#5153; RRID:AB_10691711 PSMA (D7I8E); Rabbit Monoclonal; Cell Signaling Cat#12815; RRID:AB_2798032 FKBP5; Rabbit Polyclonal Cell Signaling Cat#8245; RRID:AB_10831198 PLZF (D8G3G); Rabbit Monoclonal Cell Signaling Cat#39784; RRID:AB_2799161 Phospho-AKT (Ser473) (D9E); Rabbit Monoclonal Cell Signaling Cat#4060; RRID:AB_2315049 AKT (pan) (11E7); Rabbit Monoclonal Cell Signaling Cat#4685; RRID:AB_2225340 Phospho-Src Family (Tyr416) (D49G4); Rabbit Monoclonal Cell Signaling Cat# 6943; RRID:AB_10013641 Src (L4A1); Mouse Monoclonal Cell Signaling Cat#2110; RRID:AB_10691385 PTEN; Rabbit Polyclonal Cell Signaling Cat#9552; RRID:AB_10694066 KI-67 (30-9); Rabbit Monoclonal Ventana/Roche Cat#790–4286 Vimentin; Rabbit Monoclonal Cell Signaling Cat#5741; RRID:AB_10695459 E-cadherin; Mouse Monoclonal BD Transduction Laboratories Cat#610181; RRID:AB_397580 b-Catenin; Rabbit Polyclonal Zymed Cat# 71–2700 GAPDH; Mouse Monoclonal Santacruz Cat#sc-365062; RRID:AB_10847862 CD4 (D7D2Z); Rabbit Monoclonal Cell Signaling Cat#25229; RRID:AB_2798898 CD8α (D4W2Z); Rabbit Monoclonal Cell Signaling Cat#98941; RRID:AB_2756376 Chromogranin A (CgA); Rabbit Monoclonal Abcam Cat# ab15160; RRID:AB_301704 Bacterial and virus strains One ShotTM TOP10 Chemically Competent E. coli Thermofisher Scientific Cat#C404003 Chemicals, peptides, and recombinant proteins R1881 Millipore Sigma Cat#R0908 Enzalutamide (MDV3100) Selleckchem Cat#S2840 Apalutamide (ARN-509) Selleckchem Cat#S1060 Darolutamide (ODM-201) Selleckchem Cat#S7559 Zelavespib (PU-H71) Selleckchem Cat#S8039 MK-2206 Selleckchem Cat#S1078 Insulin, human recombinant Thermofisher Scientific Cat#12585014 Venetoclax (ABT-199) Selleckchem Cat#S8048 Navitoclax (ABT-263) Selleckchem Cat#S1001 Vismodegib (GDC-0449) Selleckchem Cat#S1082 (Continued on next page) Cell Reports 44, 115779, June 24, 2025 21 .. REAGENT or RESOURCE SOURCE IDENTIFIER Talazoparib (BMN 673) Selleckchem Cat#S7048 Critical commercial assays Direct-zol RNA Kit Zymo Research Cat#R2071 qScript cDNA SuperMix Quantabio Cat#95048–100 MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) assay Thermofisher Scientific Cat#M6494 Muse® Cell Cycle kit Cytek Cat#MCH100106 Muse® Caspase-3/7 kit Cytek Cat#MCH100108 Deposited data RNA Seq_1 BCL2 Overexpression This Paper GSE273736 RNA Seq_2 Androgen Stimulation This Paper GSE273737 RNA Seq_3 Venetoclax Treatment This Paper GSE273738 Experimental models: Cell lines LNCaP ATCC Cat#CRL-1740 LNCaP C-42 ATCC Cat#CRL-3314 22RV1 ATCC Cat#CRL-2505 DU145 ATCC Cat#HTB-81 PC3 ATCC Cat#CRL-1435 VCaP ATCC Cat#CRL-2876 RWPE1 ATCC Cat#CRL-3607 MDA-MB-231 ATCC Cat#CRM-HTB-26 MYCCaP ATCC Cat#CRL-3255 293FT Thermofisher Scientific Cat#R70007 LNCaP-abl Zoran Culig NA LAPC4 Charles Sawyers NA PC3M Raymond C. Bergan NA Experimental models: Organisms/strains FVB/NJ Jackson Laboratory Cat#IMSR_JAX:001800 C57BL/6J Jackson Laboratory Cat#IMSR_JAX:000664 Oligonucleotides BCL2; Human Thermofisher Scientific Cat#Hs04986394_s1 BCLXL; Human Thermofisher Scientific Cat#Hs00236329_m1 KLK3/PSA; Human Thermofisher Scientific Cat#Hs02576345_m1 BCL2; Mouse Thermofisher Scientific Cat#Mm00477631_m1 BCLXL; Mouse Thermofisher Scientific Cat#Mm00437783_m1 PTEN; Mouse Thermofisher Scientific Cat#Mm00477208_m1 PTEN; Human Thermofisher Scientific Cat#Hs02621230_s1 AR; Human Thermofisher Scientific Cat#Hs00171172_m1 FOLH1/PSMA; Human Thermofisher Scientific Cat#Hs00379515_m1 PLZF; Human Thermofisher Scientific Cat#Hs00232313_m1 Vimentin; Human Thermofisher Scientific Cat#Hs00958111_m1 GAPDH; Human Thermofisher Scientific Cat#Hs02786624_g1 E cadherin; Human Thermofisher Scientific Cat#Hs01023895_m1 GAPDH; Mouse Thermofisher Scientific Cat#Mm05724508_g1 BCL2 siRNA Human Horizon/Dharmacon Cat#L-003307 AR siRNA Human Horizon/Dharmacon Cat#L-003400 SNAIL siRNA Human Horizon/Dharmacon Cat#L-010847 FOXA1 siRNA Human Horizon/Dharmacon Cat#L-010319 (Continued on next page) 22 Cell Reports 44, 115779, June 24, 2025

Recombinant:

Cell-Signaling:

Article Title: Development and validation of a PSMA-positive triple-negative breast cancer mouse model for preclinical targeted radionuclide therapies
Article Snippet: Tumor sections were then incubated overnight at 4 °C with the primary antibody in the staining solution (PBS 1X-BSA (1%)-Biotin (Vector laboratories, SP-2001)). .. We used the following antibodies (i) anti-CD31 antibody ( 1/2000 , Abcam # ab182981) (ii) anti-Ki-67 antibody ( 1/100 , Abcam # ab15580) (iii) anti-PSMA antibody ( 1/100 , Cell Signaling Technology #12815) (iv) anti-HIF-1a ( 1/100 , Cell Signaling Technology #48085). .. After rinsing, the tissue sections were stained with a biotinylated goat anti-rabbit IgG antibody ( 1/500 , Invitrogen # 656140) for one hour at RT.

Membrane:

Article Title: Proteomic Profiling of Extracellular Vesicles Distinguishes Prostate Cancer Molecular Subtypes.
Article Snippet: Lysates were then quantified using BCA assay (ThermoFisher) according to manufacturer recommendations. .. Samples were run and transferred onto PVDF 0.45 μm membrane (Millipore) and blocked with 5% BSA Fraction V. Primary antibodies included CD9 (Cell Signalling, 13174), ALIX (Cell Signalling, 2171), Calnexin (Cell Signalling, 2679), PSMA (Cell Signalling, 12815), TROP2 (Abcam SP295). .. Secondary antibodies were Licor IRconjugated, and membranes were imaged using the BioRad Chemi-Doc Imager.

Article Title: Proteomic profiling of extracellular vesicles distinguishes prostate cancer molecular subtypes
Article Snippet: Lysates were then quantified using BCA assay (ThermoFisher) according to manufacturer recommendations. .. Samples were run and transferred onto PVDF 0.45um membrane (Millipore) and blocked with 5% BSA Fraction V. Primary antibodies included CD9 (Cell Signaling, 13174), ALIX (Cell Signaling, 2171), Calnexin (Cell Signaling, 2679), PSMA (Cell Signaling, 12815), TROP2 (abcam SP295). .. Secondary antibodies were Licor IR-conjugated, and membranes were imaged using the BioRad Chemi-Doc Imager.

Article Title: Proteomic Profiling of Extracellular Vesicles Distinguishes Prostate Cancer Molecular Subtypes
Article Snippet: Lysates were then quantified using BCA assay (ThermoFisher) according to manufacturer recommendations. .. Samples were run and transferred onto PVDF 0.45 μm membrane (Millipore) and blocked with 5% BSA Fraction V. Primary antibodies included CD9 (Cell Signalling, 13174), ALIX (Cell Signalling, 2171), Calnexin (Cell Signalling, 2679), PSMA (Cell Signalling, 12815), TROP2 (Abcam SP295). .. Secondary antibodies were Licor IR‐conjugated, and membranes were imaged using the BioRad Chemi‐Doc Imager.

Review