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hif1α cdna  (OriGene)


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    Structured Review

    OriGene hif1α cdna
    Hif1α Cdna, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hif+1a/HIF-1+alpha+(HIF1A)+(NM_181054)+Human+Untagged+Clone/pm28196140-217-15-18
    Average 92 stars, based on 4 article reviews
    hif1α cdna - by Bioz Stars, 2026-10
    92/100 stars

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    Related Articles

    Sequencing:

    Article Title: Hypoxia induced upregulation and function of the thiamine transporter, SLC19A3 in a breast cancer cell line.
    Article Snippet: .. A scrambled sequence and four different 29mer HIF-1α shRNA constructs in pGFP-V-RS vector targeted against accession #NM_001530 and NM_181054 were obtained from OriGene (Rockville, MD). .. BT474 cells were seeded into a 6 well plate and were individually transfected with 2 μg of each construct using FuGENE HD (Roche Applied Science, Indianapolis, IN) according to the manufacturer’s Figure 6.

    shRNA:

    Article Title: Hypoxia induced upregulation and function of the thiamine transporter, SLC19A3 in a breast cancer cell line.
    Article Snippet: .. A scrambled sequence and four different 29mer HIF-1α shRNA constructs in pGFP-V-RS vector targeted against accession #NM_001530 and NM_181054 were obtained from OriGene (Rockville, MD). .. BT474 cells were seeded into a 6 well plate and were individually transfected with 2 μg of each construct using FuGENE HD (Roche Applied Science, Indianapolis, IN) according to the manufacturer’s Figure 6.

    Construct:

    Article Title: Hypoxia induced upregulation and function of the thiamine transporter, SLC19A3 in a breast cancer cell line.
    Article Snippet: .. A scrambled sequence and four different 29mer HIF-1α shRNA constructs in pGFP-V-RS vector targeted against accession #NM_001530 and NM_181054 were obtained from OriGene (Rockville, MD). .. BT474 cells were seeded into a 6 well plate and were individually transfected with 2 μg of each construct using FuGENE HD (Roche Applied Science, Indianapolis, IN) according to the manufacturer’s Figure 6.

    Polymerase Chain Reaction:

    Article Title: Hypoxia inducible factors regulate the transcription of the sprouty2 gene and expression of the sprouty2 protein.
    Article Snippet: Antibodies used for Western blotting and chromatin immunoprecipitation were from the following companies: Sprouty2 (against N-terminus, Sigma), HIF1α (BD Transduction Laboratories), HIF2α (R&D Systems), HIF1β (Santa Cruz Biotechnology), and DNMT1 (AbCam). .. Plasmids Human full-length HIF1α is PCR amplified with primers carrying HindIII and NotI sites from HIF1α cDNA clone (OriGene Technologies, Inc.) and inserted in pcDNA3 at HindIII and NotI sites. .. HIF2α is PCR amplified with primers harboring BamHI and NotI sites from pOTB7-HIF2α (Thermo Scientific.) and inserted in pcDNA3 at the corresponding sites.

    Amplification:

    Article Title: Hypoxia inducible factors regulate the transcription of the sprouty2 gene and expression of the sprouty2 protein.
    Article Snippet: Antibodies used for Western blotting and chromatin immunoprecipitation were from the following companies: Sprouty2 (against N-terminus, Sigma), HIF1α (BD Transduction Laboratories), HIF2α (R&D Systems), HIF1β (Santa Cruz Biotechnology), and DNMT1 (AbCam). .. Plasmids Human full-length HIF1α is PCR amplified with primers carrying HindIII and NotI sites from HIF1α cDNA clone (OriGene Technologies, Inc.) and inserted in pcDNA3 at HindIII and NotI sites. .. HIF2α is PCR amplified with primers harboring BamHI and NotI sites from pOTB7-HIF2α (Thermo Scientific.) and inserted in pcDNA3 at the corresponding sites.

    Over Expression:

    Article Title: Hypoxia represses FOXF1 in lung endothelial cells through HIF-1α
    Article Snippet: .. For overexpression of HIF-1α, cells were transfected with CMV-HIF-1α (Origene, Cat# MG210895). .. Cells were lysed for RNA and protein using lysis buffer (Qiagen, cat#74104) and RIPA buffer (Cell Signaling Technology, Cat#9806) respectively, as per the manufacturer’s protocol. qRT-PCR was performed as previously described ( ) using TaqMan probes for mouse Foxf1 (Mm00487497_m1), mouse Hif1α (Mm00468869_m1), mouse ActB (Mm00607939_s1), human FOXF1 (Hs00230962_m1), human HIF1α (Hs00153153_m1) and human ACTB (Hs99999903_m1).

    Transfection:

    Article Title: Hypoxia represses FOXF1 in lung endothelial cells through HIF-1α
    Article Snippet: .. For overexpression of HIF-1α, cells were transfected with CMV-HIF-1α (Origene, Cat# MG210895). .. Cells were lysed for RNA and protein using lysis buffer (Qiagen, cat#74104) and RIPA buffer (Cell Signaling Technology, Cat#9806) respectively, as per the manufacturer’s protocol. qRT-PCR was performed as previously described ( ) using TaqMan probes for mouse Foxf1 (Mm00487497_m1), mouse Hif1α (Mm00468869_m1), mouse ActB (Mm00607939_s1), human FOXF1 (Hs00230962_m1), human HIF1α (Hs00153153_m1) and human ACTB (Hs99999903_m1).

    Synthesized:

    Article Title: HIF Inactivation of p53 in Ovarian Cancer Can Be Reversed by Topotecan, Restoring Cisplatin and Paclitaxel Sensitivity
    Article Snippet: 28 Ovarian cancer (OC) growth under hypoxic conditions results in hypoxia inducible factor-1α 29 (HIF-1α) stabilization.. HIF-1α is an adverse prognostic factor that may contribute to worse 30 outcomes via its capacity to bind to p53, potentially blocking p53-mediated apoptosis.. We 31 determined whether HIF-1α-p53 binding occurred in hypoxic OC cell lines, and if this blocked 32 p53 transcriptional activity.



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