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hif 1α  (OriGene)


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    Structured Review

    OriGene hif 1α
    Hif 1α, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hif+1a/HIF-1+alpha+(HIF1A)+(NM_001530)+Human+Untagged+Clone/pmc04965575-657-29-32
    Average 92 stars, based on 12 article reviews
    hif 1α - by Bioz Stars, 2026-10
    92/100 stars

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    Related Articles

    Over Expression:

    Article Title: Hypoxia-inducible factor-1 promotes cancer progression through activating AKT/Cyclin D1 signaling pathway in osteosarcoma.
    Article Snippet: Objective: Osteosarcoma is an aggressive malignant neoplasm, which commonly afflicts patients of 20–30 years of age, and its morbidity has increased markedly in recent years.. Certain genes and signal pathways have been identified to exert key roles in osteosarcoma progression.. Here, we set out to characterize in more detail of the role of HIF-1/AKT/Cyclin D1 pathway in the progression of osteosarcoma.

    Control:

    Article Title: Hypoxia-inducible factor-1 promotes cancer progression through activating AKT/Cyclin D1 signaling pathway in osteosarcoma.
    Article Snippet: Objective: Osteosarcoma is an aggressive malignant neoplasm, which commonly afflicts patients of 20–30 years of age, and its morbidity has increased markedly in recent years.. Certain genes and signal pathways have been identified to exert key roles in osteosarcoma progression.. Here, we set out to characterize in more detail of the role of HIF-1/AKT/Cyclin D1 pathway in the progression of osteosarcoma.

    Article Title: Activation of Epidermal Growth Factor Receptor/p38/Hypoxia-inducible Factor-1α Is Pivotal for Angiogenesis and Tumorigenesis of Malignantly Transformed Cells Induced by Hexavalent Chromium
    Article Snippet: Moloney murine leukemia virus reverse transcriptase was from Promega (Madison, WI). .. Human angiogenesis array C1000 was from RayBiotech (Norcross, GA). qScript cDNA synthesis kit and Perfecta Sybr Green Fastmix were from Quanta Biosciences (Gaithersburg, MD). shRNAs of EGFR, MMP-1, and HIF-1α were from Origene (Rockville, MD). p3XFLAG-CMV/EGFR and its control plasmids were kindly provided by Dr. Qiou Wei (Department of Toxicology and Cancer Biology, University of Kentucky) ( 58 ). .. Bradford protein assay reagent was from Bio-Rad.

    Article Title: Estrogen-related receptor α is involved in angiogenesis and skeletal muscle revascularization in hindlimb ischemia
    Article Snippet: .. Cells were transfected with the Vegfa promoter-driven firefly luciferase (100 ng) along with a control plasmid (pCMX, 80 ng), ERRα plasmid (pCMX-ERRα, 80 ng), or HIF1α plasmid (# sc119189, Origene Rockville, MD, 80 ng) using lipofectamine 3000, according to the manufacturer’s instructions (Invitrogen). ..

    cDNA Synthesis:

    Article Title: Activation of Epidermal Growth Factor Receptor/p38/Hypoxia-inducible Factor-1α Is Pivotal for Angiogenesis and Tumorigenesis of Malignantly Transformed Cells Induced by Hexavalent Chromium
    Article Snippet: Moloney murine leukemia virus reverse transcriptase was from Promega (Madison, WI). .. Human angiogenesis array C1000 was from RayBiotech (Norcross, GA). qScript cDNA synthesis kit and Perfecta Sybr Green Fastmix were from Quanta Biosciences (Gaithersburg, MD). shRNAs of EGFR, MMP-1, and HIF-1α were from Origene (Rockville, MD). p3XFLAG-CMV/EGFR and its control plasmids were kindly provided by Dr. Qiou Wei (Department of Toxicology and Cancer Biology, University of Kentucky) ( 58 ). .. Bradford protein assay reagent was from Bio-Rad.

    SYBR Green Assay:

    Article Title: Activation of Epidermal Growth Factor Receptor/p38/Hypoxia-inducible Factor-1α Is Pivotal for Angiogenesis and Tumorigenesis of Malignantly Transformed Cells Induced by Hexavalent Chromium
    Article Snippet: Moloney murine leukemia virus reverse transcriptase was from Promega (Madison, WI). .. Human angiogenesis array C1000 was from RayBiotech (Norcross, GA). qScript cDNA synthesis kit and Perfecta Sybr Green Fastmix were from Quanta Biosciences (Gaithersburg, MD). shRNAs of EGFR, MMP-1, and HIF-1α were from Origene (Rockville, MD). p3XFLAG-CMV/EGFR and its control plasmids were kindly provided by Dr. Qiou Wei (Department of Toxicology and Cancer Biology, University of Kentucky) ( 58 ). .. Bradford protein assay reagent was from Bio-Rad.

    Plasmid Preparation:

    Article Title: Midkine Is a Potential Therapeutic Target of Tumorigenesis, Angiogenesis, and Metastasis in Non-Small Cell Lung Cancer
    Article Snippet: .. HIF-1α plasmid (SC119189) and MDK plasmid (SC319913) were purchased from OriGene Technologies, Inc. (Rockville, MD, USA). .. Small interfering (si)RNAS, siHIF-1α (sc-35561), siHIF-2α (sc-35316), and siMDK (sc-39711) were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA).

    Article Title: Estrogen-related receptor α is involved in angiogenesis and skeletal muscle revascularization in hindlimb ischemia
    Article Snippet: .. Cells were transfected with the Vegfa promoter-driven firefly luciferase (100 ng) along with a control plasmid (pCMX, 80 ng), ERRα plasmid (pCMX-ERRα, 80 ng), or HIF1α plasmid (# sc119189, Origene Rockville, MD, 80 ng) using lipofectamine 3000, according to the manufacturer’s instructions (Invitrogen). ..

    Transfection:

    Article Title: Estrogen-related receptor α is involved in angiogenesis and skeletal muscle revascularization in hindlimb ischemia
    Article Snippet: .. Cells were transfected with the Vegfa promoter-driven firefly luciferase (100 ng) along with a control plasmid (pCMX, 80 ng), ERRα plasmid (pCMX-ERRα, 80 ng), or HIF1α plasmid (# sc119189, Origene Rockville, MD, 80 ng) using lipofectamine 3000, according to the manufacturer’s instructions (Invitrogen). ..

    Luciferase:

    Article Title: Estrogen-related receptor α is involved in angiogenesis and skeletal muscle revascularization in hindlimb ischemia
    Article Snippet: .. Cells were transfected with the Vegfa promoter-driven firefly luciferase (100 ng) along with a control plasmid (pCMX, 80 ng), ERRα plasmid (pCMX-ERRα, 80 ng), or HIF1α plasmid (# sc119189, Origene Rockville, MD, 80 ng) using lipofectamine 3000, according to the manufacturer’s instructions (Invitrogen). ..



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