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hif 1α expression plasmid  (OriGene)


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    OriGene hif 1α expression plasmid
    Hif 1α Expression Plasmid, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hif+1a/HIF-1+alpha+(HIF1A)+(NM_001530)+Human+Tagged+ORF+Clone/pmc05371873-83-3-9
    Average 90 stars, based on 1 article reviews
    hif 1α expression plasmid - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Cloning:

    Article Title: KDM2 Family Members are Regulated by HIF-1 in Hypoxia
    Article Snippet: Images were deconvolved and analysed using OMERO client software (5.2.7, Open Microscopy Environment, Dundee, UK) [ ]. .. Plasmids and cloning: HIF-1α expression plasmid was obtained from Origene. .. KDM2A HRE sites were cloned using Kpn I and Mlu I restriction enzymes in the pGL3 promoter luciferase construct (Promega) using the following oligonucleotides WT KDM2A (−186 GCGTG, −143 GCGTG) Forward: 5’-CGCATCCGCGCGTGCGCGCGCGGAGTGACGCGTGCGCGTTCA-3’ Reverse: 5’-CGCGTGAACGCGCACGCGTCACTCCGCGCGCGCACGCGCGGATGCGGTAC-3’ KDM2A −186 mut (−186 GATAA, −143 GCGTG) Forward: 5’-CGCATCCGCGATAACGCGCGCGGAGTGACGCGTGCGCGTTCA-3’ Reverse: 5’-CGCGTGAACGCGCACGCGTCACTCCGCGCGCGTTATCGCGGATGCGGTAC-3’ KDM2A double mut (−186 GATAA, −143 GATAA) Forward: 5’-CGCATCCGCGATAACGCGCGCGGAGTGACGATAACGCGTTCA-3’ Reverse: 5’-CGCGTGAACGCGTTATCGTCACTCCGCGCGCGTTATCGCGGATGCGGTAC-3’ Luciferase reporter assay: Cells (2 × 10 5 ) were seeded in six-well plates and transfected with 1 μg DNA.

    Expressing:

    Article Title: KDM2 Family Members are Regulated by HIF-1 in Hypoxia
    Article Snippet: Images were deconvolved and analysed using OMERO client software (5.2.7, Open Microscopy Environment, Dundee, UK) [ ]. .. Plasmids and cloning: HIF-1α expression plasmid was obtained from Origene. .. KDM2A HRE sites were cloned using Kpn I and Mlu I restriction enzymes in the pGL3 promoter luciferase construct (Promega) using the following oligonucleotides WT KDM2A (−186 GCGTG, −143 GCGTG) Forward: 5’-CGCATCCGCGCGTGCGCGCGCGGAGTGACGCGTGCGCGTTCA-3’ Reverse: 5’-CGCGTGAACGCGCACGCGTCACTCCGCGCGCGCACGCGCGGATGCGGTAC-3’ KDM2A −186 mut (−186 GATAA, −143 GCGTG) Forward: 5’-CGCATCCGCGATAACGCGCGCGGAGTGACGCGTGCGCGTTCA-3’ Reverse: 5’-CGCGTGAACGCGCACGCGTCACTCCGCGCGCGTTATCGCGGATGCGGTAC-3’ KDM2A double mut (−186 GATAA, −143 GATAA) Forward: 5’-CGCATCCGCGATAACGCGCGCGGAGTGACGATAACGCGTTCA-3’ Reverse: 5’-CGCGTGAACGCGTTATCGTCACTCCGCGCGCGTTATCGCGGATGCGGTAC-3’ Luciferase reporter assay: Cells (2 × 10 5 ) were seeded in six-well plates and transfected with 1 μg DNA.

    Plasmid Preparation:

    Article Title: KDM2 Family Members are Regulated by HIF-1 in Hypoxia
    Article Snippet: Images were deconvolved and analysed using OMERO client software (5.2.7, Open Microscopy Environment, Dundee, UK) [ ]. .. Plasmids and cloning: HIF-1α expression plasmid was obtained from Origene. .. KDM2A HRE sites were cloned using Kpn I and Mlu I restriction enzymes in the pGL3 promoter luciferase construct (Promega) using the following oligonucleotides WT KDM2A (−186 GCGTG, −143 GCGTG) Forward: 5’-CGCATCCGCGCGTGCGCGCGCGGAGTGACGCGTGCGCGTTCA-3’ Reverse: 5’-CGCGTGAACGCGCACGCGTCACTCCGCGCGCGCACGCGCGGATGCGGTAC-3’ KDM2A −186 mut (−186 GATAA, −143 GCGTG) Forward: 5’-CGCATCCGCGATAACGCGCGCGGAGTGACGCGTGCGCGTTCA-3’ Reverse: 5’-CGCGTGAACGCGCACGCGTCACTCCGCGCGCGTTATCGCGGATGCGGTAC-3’ KDM2A double mut (−186 GATAA, −143 GATAA) Forward: 5’-CGCATCCGCGATAACGCGCGCGGAGTGACGATAACGCGTTCA-3’ Reverse: 5’-CGCGTGAACGCGTTATCGTCACTCCGCGCGCGTTATCGCGGATGCGGTAC-3’ Luciferase reporter assay: Cells (2 × 10 5 ) were seeded in six-well plates and transfected with 1 μg DNA.



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