Review



wildtype ha hif 1a  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    Addgene inc wildtype ha hif 1a
    Wildtype Ha Hif 1a, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 105 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hif+1a/HA-HIF1alpha-pcDNA3+(Plasmid+%2318949)/bio_rxiv__64898__2026__03__16__712182-243-11-14
    Average 94 stars, based on 105 article reviews
    wildtype ha hif 1a - by Bioz Stars, 2026-10
    94/100 stars

    Images

    Related Articles

    Transfection:

    Article Title: Nuclear Fetuin-A Drives Adipocyte Senescence via HIF-1α During Obesity
    Article Snippet: .. The transfection mixture was then added to the cells containing complete growth medium and incubated for 48 h. Cells were washed, fresh media was added and used for different treatments. pCAG-HIF1 alpha was a gift from Connie Cepko (Addgene plasmid # 21101 ; http://n2t.net/addgene:21101 ; RRID:Addgene_21101) , AHSG-bio-His was a gift from Gavin Wright (Addgene plasmid # 52175 ; http://n2t.net/addgene:52175 ; RRID:Addgene_52175) , (Addgene plasmid # 18949 ; http://n2t.net/addgene:18949 ; RRID:Addgene_18949) , PBS/pU6-HIF1 alpha RNAi plasmid 1 was a gift from Connie Cepko (Addgene plasmid # 21103 ; http://n2t.net/addgene:21103 ; RRID:Addgene_21103)( ). ..

    Incubation:

    Article Title: Nuclear Fetuin-A Drives Adipocyte Senescence via HIF-1α During Obesity
    Article Snippet: .. The transfection mixture was then added to the cells containing complete growth medium and incubated for 48 h. Cells were washed, fresh media was added and used for different treatments. pCAG-HIF1 alpha was a gift from Connie Cepko (Addgene plasmid # 21101 ; http://n2t.net/addgene:21101 ; RRID:Addgene_21101) , AHSG-bio-His was a gift from Gavin Wright (Addgene plasmid # 52175 ; http://n2t.net/addgene:52175 ; RRID:Addgene_52175) , (Addgene plasmid # 18949 ; http://n2t.net/addgene:18949 ; RRID:Addgene_18949) , PBS/pU6-HIF1 alpha RNAi plasmid 1 was a gift from Connie Cepko (Addgene plasmid # 21103 ; http://n2t.net/addgene:21103 ; RRID:Addgene_21103)( ). ..

    Plasmid Preparation:

    Article Title: Nuclear Fetuin-A Drives Adipocyte Senescence via HIF-1α During Obesity
    Article Snippet: .. The transfection mixture was then added to the cells containing complete growth medium and incubated for 48 h. Cells were washed, fresh media was added and used for different treatments. pCAG-HIF1 alpha was a gift from Connie Cepko (Addgene plasmid # 21101 ; http://n2t.net/addgene:21101 ; RRID:Addgene_21101) , AHSG-bio-His was a gift from Gavin Wright (Addgene plasmid # 52175 ; http://n2t.net/addgene:52175 ; RRID:Addgene_52175) , (Addgene plasmid # 18949 ; http://n2t.net/addgene:18949 ; RRID:Addgene_18949) , PBS/pU6-HIF1 alpha RNAi plasmid 1 was a gift from Connie Cepko (Addgene plasmid # 21103 ; http://n2t.net/addgene:21103 ; RRID:Addgene_21103)( ). ..

    Article Title: The pyruvate branch point controls lymphoid cancer cell dissemination
    Article Snippet: .. The plasmids for prolyl hydroxylation-defective HA-HIF-1a (P402A/P564A-pcDNA3, Addgene plasmid #18955) and wildtype HA-HIF-1a (pcDNA3, Addgene plasmid #18949) were generously provided by William Kaelin. .. Transfection of cells was carried out using the TurboFect Transfection Reagent (ThermoFisher Scientific, Waltham, MA) as per the manufacturer’s instructions.

    Article Title: Proteomic analysis reveals inhibition of mevalonate and glycolysis pathways in hepatocytes by 27-hydroxycholesterol
    Article Snippet: 27OHC was purchased from Tocris Bioscience (cat. no. 3907). .. HA-HIF1alpha-pcDNA3 [ ] was a gift from William Kaelin (Addgene plasmid # 18949) ..

    Article Title: Oncogenic role of C11orf86 in ovarian cancer involving hypoxia response and immune modulation
    Article Snippet: Cells were then transfected with 80 μM of siRNA using the X-tremeGENE siRNA Transfection Reagent (Roche; Indianapolis, IN, USA) according to the manufacturer’s instructions. .. For HIF-1α overexpression, the HA-HIF-1α-pcDNA3.0 plasmid (#18949) was a gift from William Kaelin [ ] (Addgene plasmid #18949; http://n2t.net/addgene:18949 ; RRID: Addgene_18949). ..

    Article Title: KDM2B controls HIF levels and activity through its JmjC and CxxC domains
    Article Snippet: .. HA-HIF1alpha-pcDNA3 was a gift from William Kaelin (Addgene plasmid # 18949). .. Site-directed mutagenesis was performed using a SureCylcer 8800 thermocycler (Agilent) with the KOD hot start DNA polymerase kit.

    Article Title: MicroRNA-210 Suppresses NF-κB Signaling in Lipopolysaccharide-Stimulated Dental Pulp Cells Under Hypoxic Conditions
    Article Snippet: Densitometric analysis was performed using ImageJ software (version 2; National Institutes of Health, Bethesda, MD, USA) to quantify band intensity and determine the relative ratios. .. To induce transient expression of HIF1α, a mammalian expression vector containing the cytomegalovirus promoter and encoding an oxygen-insensitive mutant form of HIF-1α (HA-HIF1α-P402A/P564A mutant-pcDNA3, a gift from William Kaelin, Addgene plasmid #18955; https://www.addgene.org/18955/ ; accessed on 18 June 2018; RRID: Addgene_18955, Watertown, MA, USA [ ]) was used. ..

    Over Expression:

    Article Title: Oncogenic role of C11orf86 in ovarian cancer involving hypoxia response and immune modulation
    Article Snippet: Cells were then transfected with 80 μM of siRNA using the X-tremeGENE siRNA Transfection Reagent (Roche; Indianapolis, IN, USA) according to the manufacturer’s instructions. .. For HIF-1α overexpression, the HA-HIF-1α-pcDNA3.0 plasmid (#18949) was a gift from William Kaelin [ ] (Addgene plasmid #18949; http://n2t.net/addgene:18949 ; RRID: Addgene_18949). ..

    Expressing:

    Article Title: MicroRNA-210 Suppresses NF-κB Signaling in Lipopolysaccharide-Stimulated Dental Pulp Cells Under Hypoxic Conditions
    Article Snippet: Densitometric analysis was performed using ImageJ software (version 2; National Institutes of Health, Bethesda, MD, USA) to quantify band intensity and determine the relative ratios. .. To induce transient expression of HIF1α, a mammalian expression vector containing the cytomegalovirus promoter and encoding an oxygen-insensitive mutant form of HIF-1α (HA-HIF1α-P402A/P564A mutant-pcDNA3, a gift from William Kaelin, Addgene plasmid #18955; https://www.addgene.org/18955/ ; accessed on 18 June 2018; RRID: Addgene_18955, Watertown, MA, USA [ ]) was used. ..

    Mutagenesis:

    Article Title: MicroRNA-210 Suppresses NF-κB Signaling in Lipopolysaccharide-Stimulated Dental Pulp Cells Under Hypoxic Conditions
    Article Snippet: Densitometric analysis was performed using ImageJ software (version 2; National Institutes of Health, Bethesda, MD, USA) to quantify band intensity and determine the relative ratios. .. To induce transient expression of HIF1α, a mammalian expression vector containing the cytomegalovirus promoter and encoding an oxygen-insensitive mutant form of HIF-1α (HA-HIF1α-P402A/P564A mutant-pcDNA3, a gift from William Kaelin, Addgene plasmid #18955; https://www.addgene.org/18955/ ; accessed on 18 June 2018; RRID: Addgene_18955, Watertown, MA, USA [ ]) was used. ..



    Similar Products

    86
    Cell Signaling Technology Inc hif 1a
    Hif 1a, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hif+1a/pm41888366-104-4-7
    Average 86 stars, based on 1 article reviews
    hif 1a - by Bioz Stars, 2026-10
    86/100 stars
      Buy from Supplier

    93
    Cusabio hif 1a hif 1a concentrations
    Hif 1a Hif 1a Concentrations, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hif+1a/HIF1A/pm41878451-64-26-42
    Average 93 stars, based on 1 article reviews
    hif 1a hif 1a concentrations - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    93
    Cusabio hif 1a detection
    Hif 1a Detection, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hif+1a/HIF1A/pm41878451-64-1-42
    Average 93 stars, based on 1 article reviews
    hif 1a detection - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    93
    Cusabio human hif 1a elisa kit
    Human Hif 1a Elisa Kit, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hif+1a/Human+hypoxia-inducible+factor+1%CE%B1(HIF-1%CE%B1)+ELISA+kit/pm41878451-64-37-42
    Average 93 stars, based on 1 article reviews
    human hif 1a elisa kit - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    94
    Addgene inc wildtype ha hif 1a
    Wildtype Ha Hif 1a, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hif+1a/HA-HIF1alpha-pcDNA3+(Plasmid+%2318949)/bio_rxiv__64898__2026__03__16__712182-243-11-14
    Average 94 stars, based on 1 article reviews
    wildtype ha hif 1a - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    94
    Addgene inc prolyl hydroxylation defective ha hif 1a
    Prolyl Hydroxylation Defective Ha Hif 1a, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hif+1a/HA-HIF1alpha+P402A%2FP564A-pcDNA3+(Plasmid+%2318955)/bio_rxiv__64898__2026__03__16__712182-243-3-7
    Average 94 stars, based on 1 article reviews
    prolyl hydroxylation defective ha hif 1a - by Bioz Stars, 2026-10
    94/100 stars
      Buy from Supplier

    96
    Cell Signaling Technology Inc anti hif 1a
    Anti Hif 1a, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hif+1a/PKM2+Antibody/pm41828578-216-27-29
    Average 96 stars, based on 1 article reviews
    anti hif 1a - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    93
    Cell Signaling Technology Inc iv anti hif 1a 1 100 cell signaling technology 48085
    Iv Anti Hif 1a 1 100 Cell Signaling Technology 48085, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hif+1a/Prostate+Specific+Membrane+Antigen+XP+Rabbit+mAb/pmc13003023-105-33-38
    Average 93 stars, based on 1 article reviews
    iv anti hif 1a 1 100 cell signaling technology 48085 - by Bioz Stars, 2026-10
    93/100 stars
      Buy from Supplier

    Image Search Results