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prolyl hydroxylation defective ha hif 1a (Addgene inc)

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Addgene inc prolyl hydroxylation defective ha hif 1a
Prolyl Hydroxylation Defective Ha Hif 1a, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hif+1a/HA-HIF1alpha+P402A%2FP564A-pcDNA3+(Plasmid+%2318955)/bio_rxiv__64898__2026__03__16__712182-243-3-7
Average 94 stars, based on 66 article reviews

prolyl hydroxylation defective ha hif 1a - by Bioz Stars, 2026-10

94/100 stars

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Plasmid Preparation:

Article Title: Pseudohypoxic stabilization of HIF1α via cyclophilin D suppression promotes melanoma metastasis.
Article Snippet: .. Plasmid, antibodies, and chemicals HA-HIF1αlpha P402A/P564A-HA-pcDNA3 was a gift from William Kaelin (Addgene plasmid #18955). .. Anti-HIF2α and anti-vimentin were purchased from Cell Signaling Technology; an anti-CypD antibody was purchased from Thermo Fisher Scientific; anti-Twist, anti-Snail antibodies were purchased from ABclonal Technology; anti-E-cadherin and anti-N-cadherin antibodies were purchased from BD Biosciences; an anti-HIF1α antibody was purchased from Abcam; and an anti-β-actin antibody was purchased from MP Biomedicals.

Article Title: The pyruvate branch point controls lymphoid cancer cell dissemination
Article Snippet: .. The plasmids for prolyl hydroxylation-defective HA-HIF-1a (P402A/P564A-pcDNA3, Addgene plasmid #18955) and wildtype HA-HIF-1a (pcDNA3, Addgene plasmid #18949) were generously provided by William Kaelin. .. Transfection of cells was carried out using the TurboFect Transfection Reagent (ThermoFisher Scientific, Waltham, MA) as per the manufacturer’s instructions.

Article Title: Pseudohypoxic stabilization of HIF1α via cyclophilin D suppression promotes melanoma metastasis
Article Snippet: .. HA-HIF1αlpha P402A/P564A-HA-pcDNA3 was a gift from William Kaelin (Addgene plasmid #18955). .. Anti-HIF2α and anti-vimentin were purchased from Cell Signaling Technology; an anti-CypD antibody was purchased from Thermo Fisher Scientific; anti-Twist, anti-Snail antibodies were purchased from ABclonal Technology; anti-E-cadherin and anti-N-cadherin antibodies were purchased from BD Biosciences; an anti-HIF1α antibody was purchased from Abcam; and an anti-β-actin antibody was purchased from MP Biomedicals.

Hemagglutination Assay:

Article Title: Cholesterol accumulation-induced impairment of AKT signaling in LPS-stimulated macrophages play a dispensable role in suppressing HIF-1α-dependent glycolysis
Article Snippet: .. RAW264.7 Mφs (2 x 10 6 , ATCC, Cat# ATCC TIB-71) were electroporated (Amaxa Cell Line Nucleofector Kit V, LONZA; CatVCA-1003) with 5 μg of HA-HIF1alpha (Addgene#18949) and HA-HIF1alpha P402A/P564A-pcDNA3 (Addgene#18955). .. Transfected cells were seeded in 6-well plates and cultured in recovery medium (DMEM, 20% FBS) for 3 h, then DMEM, 10% FBS for 48 h. G418 (400 μg/mL, replaced every 3 days, Gibco) was used for selection of stable-transfected cells After one week of selection, cells were used for experiments or cultured in DMEM,10% FBS, G418 (400 μg/mL).

other:

Article Title: Hypoxic stress incites HIF1α-driven ribosome biogenesis that can be exploited by targeting RNA Polymerase I.
Article Snippet: T47D cells (ATCC # HTB-133) were cultured in RPMI medium (Thermo Fisher, Cat # 22400105) supplementedwith 10% FBS and 10 μg/mL insulin.

Article Title: Hypoxic stress incites HIF1α-driven ribosome biogenesis that can be exploited by targeting RNA Polymerase I
Article Snippet: SkBr3 cells (ATCC # HTB-30) were cultured in McCoy’s 5 A medium (Thermo Fisher, Cat # 16600082) supplemented with 10% FBS.