heparinase iii (New England Biolabs)

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New England Biolabs heparinase iii

Heparinase Iii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/heparinase/Bacteroides+Heparinase+III/pmc11031382-128-0-3
Average 94 stars, based on 39 article reviews

heparinase iii - by Bioz Stars, 2026-09

94/100 stars

Images

1) Product Images from "Receptor transfer between immune cells by autoantibody-enhanced, CD32-driven trogocytosis is hijacked by HIV-1 to infect resting CD4 T cells"

Article Title: Receptor transfer between immune cells by autoantibody-enhanced, CD32-driven trogocytosis is hijacked by HIV-1 to infect resting CD4 T cells

Journal: Cell Reports Medicine

doi: 10.1016/j.xcrm.2024.101483

Figure Legend Snippet:

Techniques Used: Purification, Virus, Recombinant, Staining, Western Blot, Cell Isolation, Isolation, Marker, BIA-KA, Software, Membrane, Cell Culture

other:

Article Title: Building a translational cancer dependency map for The Cancer Genome Atlas.
Article Snippet: For rescue experiments, HS was used at 10–50 μg ml−1 (Sigma, H7640).

Article Title: Receptor transfer between immune cells by autoantibody-enhanced, CD32-driven trogocytosis is hijacked by HIV-1 to infect resting CD4 T cells.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA pCMV6-XL4-CD32A Origene Cat#SC112914 pCMV6-XL5-CD32B Origene Cat#SC128159 pCMV6-XL5-CD32C Origene Cat#SC124933 pCMV-CD64A-GFP Origene Cat#RG207487 pCMV-CD16A-GFP Origene Cat#RG219204 pBK-CMV-FynN18-GFP O.T.

Incubation:

Article Title: Functional dissection of the spike glycoprotein S1 subunit and identification of cellular cofactors for regulation of swine acute diarrhea syndrome coronavirus entry.
Article Snippet: Swine acute diarrhea syndrome coronavirus (SADS-CoV) is a novel porcine enteric coronavirus, and the broad interspecies infection of SADS-CoV poses a potential threat to human health.. This study provides experimental evidence to dissect the roles of distinct domains within the SADS-CoV spike S1 subunit in cellular entry.. Specifically, we expressed the S1 and its subdomains, S1 and S1.

Virus:

Article Title: Receptor transfer between immune cells by autoantibody-enhanced, CD32-driven trogocytosis is hijacked by HIV-1 to infect resting CD4 T cells.
Article Snippet: For all HIV-1 binding assays the culture medium was CO2-independent medium (Thermo Fischer Scientific). .. The enzymatic digestion with 1 U/ml of Heparinase I/II/III (Heparinase I [Cat. No. P0735S]; Heparinase II [Cat. No. P0736S]; Heparinase III [Cat. No. P0737S]; New England Biolabs) and Chondroitinase (Chondroitinase ABC from Proteus vulgaris [Cat. No. C3667-5UN] Merck) was performed for 15 min at 37 C in PBS stopping the reaction by addition of medium supplemented with 10% FBS followed by a washing step with PBS, prior to virus inoculation. .. For all HIV-1 binding assays the culture medium was CO2-independent medium (Thermo Fischer Scientific).

Saline:

Article Title: Trauma promotes heparan sulfate modifications and cleavage that disrupt homeostatic gene expression in microvascular endothelial cells
Article Snippet: Primary human lung microvascular ECs (HLMVEC) (S540-05a, Cell Applications, San Diego, CA, United States) were conditioned under shear stress (15 dyn/cm 2 ) for 48 h as described previously ( ). .. After 48 h of flow conditioning, HLMVECs were exposed to vehicle (1× phosphate buffered saline) or heparinase III (500 mU/mL) (P0737L, New England BioLabs, Ipswich, MA, United States) for 6 h while 15 dyn/cm 2 shear stress was maintained. ..

Shear:

FACS:

Article Title: Building a translational cancer dependency map for The Cancer Genome Atlas
Article Snippet: Flow cytometry analysis of sulfonated HSPGs was performed with the 10E4 antibody conjugated to FITC and used at 1:200 dilution (US Biological Life Sciences, H1890-10) (Extended Data Fig. ). .. Bacteroides heparinase III was obtained from New England Biolabs (P0737L) and used as per manufacturer’s protocol by treating cells for 1 h in reaction buffer at 30 °C before FACS analysis. .. Spheroid cultures were performed on ultra-low attachment 96-well plates (Corning, 7007), growth was tracked on Incucyte S3 (Sartorius) and CellTiterGlo (CTG) readouts were performed for viability measurements (Promega, G9681).

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